Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038651.t1 | XP_003591980.1 | 92.4 | 132 | 10 | 0 | 1 | 132 | 55 | 186 | 2.70E-66 | 261.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038651.t1 | G7I9N4 | 92.4 | 132 | 10 | 0 | 1 | 132 | 55 | 186 | 1.9e-66 | 261.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene038651 | MS.gene33994 | PPI |
| MS.gene038651 | MS.gene043625 | PPI |
| MS.gene041741 | MS.gene038651 | PPI |
| MS.gene038651 | MS.gene006013 | PPI |
| MS.gene33997 | MS.gene038651 | PPI |
| MS.gene006014 | MS.gene038651 | PPI |
| MS.gene33993 | MS.gene038651 | PPI |
| MS.gene54750 | MS.gene038651 | PPI |
| MS.gene041740 | MS.gene038651 | PPI |
| MS.gene038651 | MS.gene041739 | PPI |
| MS.gene059328 | MS.gene038651 | PPI |
| MS.gene059329 | MS.gene038651 | PPI |
| MS.gene33992 | MS.gene038651 | PPI |
| MS.gene038651 | MS.gene006015 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038651.t1 | MTR_1g095890 | 92.424 | 132 | 10 | 0 | 1 | 132 | 55 | 186 | 4.46e-89 | 256 |
| MS.gene038651.t1 | MTR_1g095900 | 78.788 | 132 | 28 | 0 | 1 | 132 | 54 | 185 | 1.63e-73 | 217 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038651.t1 | AT2G35810 | 62.595 | 131 | 42 | 3 | 1 | 128 | 69 | 195 | 8.02e-56 | 173 |
| MS.gene038651.t1 | AT2G35810 | 62.595 | 131 | 42 | 3 | 1 | 128 | 69 | 195 | 8.02e-56 | 173 |
| MS.gene038651.t1 | AT2G35820 | 59.848 | 132 | 47 | 2 | 1 | 130 | 55 | 182 | 3.44e-55 | 171 |
| MS.gene038651.t1 | AT2G35830 | 63.566 | 129 | 45 | 2 | 1 | 128 | 55 | 182 | 1.51e-54 | 169 |
| MS.gene038651.t1 | AT2G35830 | 65.385 | 130 | 39 | 3 | 2 | 128 | 51 | 177 | 3.46e-54 | 168 |
| MS.gene038651.t1 | AT2G35820 | 59.848 | 132 | 47 | 2 | 1 | 130 | 145 | 272 | 1.19e-53 | 170 |
Find 30 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGGTTAAGCTTGAGAAATT+TGG | 0.260726 | 8.3:+39162473 | None:intergenic |
| GGTGGTCATGTTTGGTATCT+TGG | 0.270418 | 8.3:-39162630 | MS.gene038651:CDS |
| AATGAAATCAAAGATGATTC+AGG | 0.369755 | 8.3:-39162579 | MS.gene038651:CDS |
| GGTCCATTGGTGGTCATGTT+TGG | 0.391122 | 8.3:-39162638 | MS.gene038651:CDS |
| AACCTGAAACCTTGAAAACT+TGG | 0.407022 | 8.3:+39162496 | None:intergenic |
| ACTCAGTGCCTTGGGTCCAT+TGG | 0.468229 | 8.3:-39162651 | MS.gene038651:CDS |
| CAGTGCCTTGGGTCCATTGG+TGG | 0.479028 | 8.3:-39162648 | MS.gene038651:CDS |
| CAAGTGTGACTCAGTGCCTT+GGG | 0.479051 | 8.3:-39162659 | MS.gene038651:CDS |
| GCAAGTGTGACTCAGTGCCT+TGG | 0.493326 | 8.3:-39162660 | MS.gene038651:CDS |
| TCTATTTAAGTCTGATACAA+TGG | 0.496626 | 8.3:-39162430 | MS.gene038651:CDS |
| TCATTTGAATCAACAACTGA+TGG | 0.498895 | 8.3:+39162597 | None:intergenic |
| TTATCTACCTCCTACCATTG+AGG | 0.529148 | 8.3:-39162523 | MS.gene038651:CDS |
| AACCGTGGGACATGGCATGC+TGG | 0.544561 | 8.3:-39162456 | MS.gene038651:CDS |
| GAAAACTTGGACATCCTCAA+TGG | 0.552522 | 8.3:+39162509 | None:intergenic |
| TCAAGCTTAACCGTGGGACA+TGG | 0.558912 | 8.3:-39162464 | MS.gene038651:CDS |
| ATACCAAACATGACCACCAA+TGG | 0.562955 | 8.3:+39162635 | None:intergenic |
| TATCACACACAACTTTAAGA+AGG | 0.565816 | 8.3:-39162198 | MS.gene038651:CDS |
| CACAACTTTAAGAAGGACAA+TGG | 0.594981 | 8.3:-39162191 | MS.gene038651:CDS |
| CAAAGATGATTCAGGCAAGA+CGG | 0.600075 | 8.3:-39162571 | MS.gene038651:CDS |
| GACCAGCATGCCATGTCCCA+CGG | 0.605299 | 8.3:+39162454 | None:intergenic |
| GTGATATTAGAAAACTCAAG+TGG | 0.611037 | 8.3:+39162690 | None:intergenic |
| TCATGTTTGGTATCTTGGAG+TGG | 0.611311 | 8.3:-39162625 | MS.gene038651:CDS |
| CTTTAAGAAGGACAATGGAG+TGG | 0.614786 | 8.3:-39162186 | MS.gene038651:CDS |
| CATGACCACCAATGGACCCA+AGG | 0.617176 | 8.3:+39162643 | None:intergenic |
| AAATTTCTCAAGCTTAACCG+TGG | 0.618191 | 8.3:-39162471 | MS.gene038651:CDS |
| ACTTGGACATCCTCAATGGT+AGG | 0.620998 | 8.3:+39162513 | None:intergenic |
| ATTGTATCAGACTTAAATAG+AGG | 0.622516 | 8.3:+39162432 | None:intergenic |
| AATTTCTCAAGCTTAACCGT+GGG | 0.640376 | 8.3:-39162470 | MS.gene038651:CDS |
| TGGACATCCTCAATGGTAGG+AGG | 0.650915 | 8.3:+39162516 | None:intergenic |
| ACGGTCGTGAAGTCGCACAG+TGG | 0.761158 | 8.3:-39162552 | MS.gene038651:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTTTGGTCTTTTTTA+GGG | - | chr8.3:39162594-39162613 | MS.gene038651:CDS | 15.0% |
| !!! | TTATTTTTTTGGTCTTTTTT+AGG | - | chr8.3:39162593-39162612 | MS.gene038651:CDS | 15.0% |
| !! | ATGTTCTTTACAATAATACA+TGG | - | chr8.3:39162556-39162575 | MS.gene038651:CDS | 20.0% |
| !! | TGTTCTTTACAATAATACAT+GGG | - | chr8.3:39162557-39162576 | MS.gene038651:CDS | 20.0% |
| !!! | TTGTGCTTATCTTATTTTTT+TGG | - | chr8.3:39162582-39162601 | MS.gene038651:CDS | 20.0% |
| ! | AATGAAATCAAAGATGATTC+AGG | - | chr8.3:39162295-39162314 | MS.gene038651:intron | 25.0% |
| ! | ATTGTATCAGACTTAAATAG+AGG | + | chr8.3:39162445-39162464 | None:intergenic | 25.0% |
| ! | TCTATTTAAGTCTGATACAA+TGG | - | chr8.3:39162444-39162463 | MS.gene038651:CDS | 25.0% |
| !!! | TATGGTTTTTTATTGTGTTC+AGG | - | chr8.3:39162643-39162662 | MS.gene038651:CDS | 25.0% |
| AGAACATTGCAAATGAATTC+AGG | + | chr8.3:39162543-39162562 | None:intergenic | 30.0% | |
| CATGTAACACTTTAGTGAAA+GGG | + | chr8.3:39162518-39162537 | None:intergenic | 30.0% | |
| GTGATATTAGAAAACTCAAG+TGG | + | chr8.3:39162187-39162206 | None:intergenic | 30.0% | |
| TCATTTGAATCAACAACTGA+TGG | + | chr8.3:39162280-39162299 | None:intergenic | 30.0% | |
| ! | TATCACACACAACTTTAAGA+AGG | - | chr8.3:39162676-39162695 | MS.gene038651:CDS | 30.0% |
| !! | ATGGTGTTGTGATTTATGTA+TGG | - | chr8.3:39162625-39162644 | MS.gene038651:CDS | 30.0% |
| AAATTTCTCAAGCTTAACCG+TGG | - | chr8.3:39162403-39162422 | MS.gene038651:CDS | 35.0% | |
| AACCTGAAACCTTGAAAACT+TGG | + | chr8.3:39162381-39162400 | None:intergenic | 35.0% | |
| AATTTCTCAAGCTTAACCGT+GGG | - | chr8.3:39162404-39162423 | MS.gene038651:CDS | 35.0% | |
| ACGGTTAAGCTTGAGAAATT+TGG | + | chr8.3:39162404-39162423 | None:intergenic | 35.0% | |
| GCATGTAACACTTTAGTGAA+AGG | + | chr8.3:39162519-39162538 | None:intergenic | 35.0% | |
| TAACACTTTAGTGAAAGGGA+GGG | + | chr8.3:39162514-39162533 | None:intergenic | 35.0% | |
| ! | CACAACTTTAAGAAGGACAA+TGG | - | chr8.3:39162683-39162702 | MS.gene038651:CDS | 35.0% |
| ! | TTTTTATTGTGTTCAGGTGG+TGG | - | chr8.3:39162649-39162668 | MS.gene038651:CDS | 35.0% |
| !!! | CTTTTTTAGGGTTTAGTGGA+TGG | - | chr8.3:39162606-39162625 | MS.gene038651:CDS | 35.0% |
| !!! | GGTTTTTTATTGTGTTCAGG+TGG | - | chr8.3:39162646-39162665 | MS.gene038651:CDS | 35.0% |
| !!! | TGGTCTTTTTTAGGGTTTAG+TGG | - | chr8.3:39162602-39162621 | MS.gene038651:CDS | 35.0% |
| ATACCAAACATGACCACCAA+TGG | + | chr8.3:39162242-39162261 | None:intergenic | 40.0% | |
| CAAAGATGATTCAGGCAAGA+CGG | - | chr8.3:39162303-39162322 | MS.gene038651:intron | 40.0% | |
| GAAAACTTGGACATCCTCAA+TGG | + | chr8.3:39162368-39162387 | None:intergenic | 40.0% | |
| GTAACACTTTAGTGAAAGGG+AGG | + | chr8.3:39162515-39162534 | None:intergenic | 40.0% | |
| TCATGTTTGGTATCTTGGAG+TGG | - | chr8.3:39162249-39162268 | MS.gene038651:intron | 40.0% | |
| TTATCTACCTCCTACCATTG+AGG | - | chr8.3:39162351-39162370 | MS.gene038651:intron | 40.0% | |
| ! | CTTTAAGAAGGACAATGGAG+TGG | - | chr8.3:39162688-39162707 | MS.gene038651:CDS | 40.0% |
| ! | TGAGGATGTCCAAGTTTTCA+AGG | - | chr8.3:39162369-39162388 | MS.gene038651:intron | 40.0% |
| !! | GTCCAAGTTTTCAAGGTTTC+AGG | - | chr8.3:39162376-39162395 | MS.gene038651:intron | 40.0% |
| ACTTGGACATCCTCAATGGT+AGG | + | chr8.3:39162364-39162383 | None:intergenic | 45.0% | |
| GGTGGTCATGTTTGGTATCT+TGG | - | chr8.3:39162244-39162263 | MS.gene038651:intron | 45.0% | |
| CAAGTGTGACTCAGTGCCTT+GGG | - | chr8.3:39162215-39162234 | MS.gene038651:intron | 50.0% | |
| TCAAGCTTAACCGTGGGACA+TGG | - | chr8.3:39162410-39162429 | MS.gene038651:CDS | 50.0% | |
| TGGACATCCTCAATGGTAGG+AGG | + | chr8.3:39162361-39162380 | None:intergenic | 50.0% | |
| ! | GGTCCATTGGTGGTCATGTT+TGG | - | chr8.3:39162236-39162255 | MS.gene038651:intron | 50.0% |
| ACTCAGTGCCTTGGGTCCAT+TGG | - | chr8.3:39162223-39162242 | MS.gene038651:intron | 55.0% | |
| CATGACCACCAATGGACCCA+AGG | + | chr8.3:39162234-39162253 | None:intergenic | 55.0% | |
| GCAAGTGTGACTCAGTGCCT+TGG | - | chr8.3:39162214-39162233 | MS.gene038651:intron | 55.0% | |
| AACCGTGGGACATGGCATGC+TGG | - | chr8.3:39162418-39162437 | MS.gene038651:CDS | 60.0% | |
| ACGGTCGTGAAGTCGCACAG+TGG | - | chr8.3:39162322-39162341 | MS.gene038651:intron | 60.0% | |
| CAGTGCCTTGGGTCCATTGG+TGG | - | chr8.3:39162226-39162245 | MS.gene038651:intron | 60.0% | |
| GACCAGCATGCCATGTCCCA+CGG | + | chr8.3:39162423-39162442 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 39162166 | 39162730 | 39162166 | ID=MS.gene038651 |
| chr8.3 | mRNA | 39162166 | 39162730 | 39162166 | ID=MS.gene038651.t1;Parent=MS.gene038651 |
| chr8.3 | exon | 39162398 | 39162730 | 39162398 | ID=MS.gene038651.t1.exon1;Parent=MS.gene038651.t1 |
| chr8.3 | CDS | 39162398 | 39162730 | 39162398 | ID=cds.MS.gene038651.t1;Parent=MS.gene038651.t1 |
| chr8.3 | exon | 39162166 | 39162231 | 39162166 | ID=MS.gene038651.t1.exon2;Parent=MS.gene038651.t1 |
| chr8.3 | CDS | 39162166 | 39162231 | 39162166 | ID=cds.MS.gene038651.t1;Parent=MS.gene038651.t1 |
| Gene Sequence |
| Protein sequence |
