Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33993.t1 | XP_003591980.1 | 89.2 | 186 | 19 | 1 | 1 | 185 | 1 | 186 | 5.40E-89 | 337 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33993.t1 | G7I9N4 | 89.2 | 186 | 19 | 1 | 1 | 185 | 1 | 186 | 3.9e-89 | 337.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene041740 | MS.gene33993 | PPI |
| MS.gene33993 | MS.gene006013 | PPI |
| MS.gene059329 | MS.gene33993 | PPI |
| MS.gene33992 | MS.gene33993 | PPI |
| MS.gene059328 | MS.gene33993 | PPI |
| MS.gene33993 | MS.gene038651 | PPI |
| MS.gene33997 | MS.gene33993 | PPI |
| MS.gene33993 | MS.gene041739 | PPI |
| MS.gene33993 | MS.gene54750 | PPI |
| MS.gene006014 | MS.gene33993 | PPI |
| MS.gene33993 | MS.gene041741 | PPI |
| MS.gene33993 | MS.gene006015 | PPI |
| MS.gene33993 | MS.gene33992 | PPI |
| MS.gene54750 | MS.gene33993 | PPI |
| MS.gene33993 | MS.gene33994 | PPI |
| MS.gene33993 | MS.gene043625 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33993.t1 | MTR_1g095890 | 89.247 | 186 | 19 | 1 | 1 | 185 | 1 | 186 | 2.74e-121 | 340 |
| MS.gene33993.t1 | MTR_1g095900 | 78.919 | 185 | 39 | 0 | 1 | 185 | 1 | 185 | 9.82e-108 | 306 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33993.t1 | AT2G35810 | 64.088 | 181 | 58 | 3 | 7 | 184 | 22 | 198 | 5.93e-84 | 246 |
| MS.gene33993.t1 | AT2G35810 | 64.088 | 181 | 58 | 3 | 7 | 184 | 22 | 198 | 5.93e-84 | 246 |
| MS.gene33993.t1 | AT2G35820 | 63.333 | 180 | 60 | 2 | 7 | 184 | 8 | 183 | 2.34e-82 | 242 |
| MS.gene33993.t1 | AT2G35830 | 64.641 | 181 | 58 | 3 | 7 | 184 | 8 | 185 | 2.77e-81 | 239 |
| MS.gene33993.t1 | AT2G35820 | 63.333 | 180 | 60 | 2 | 7 | 184 | 98 | 273 | 1.34e-80 | 241 |
| MS.gene33993.t1 | AT2G35830 | 63.536 | 181 | 55 | 4 | 7 | 184 | 8 | 180 | 2.69e-77 | 229 |
Find 51 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCCAGGTCTTCAAGGTTTC+AGG | 0.178682 | 1.2:-72582629 | MS.gene33993:CDS |
| GGTGGTAATGTTTGGTATCT+TGG | 0.305200 | 1.2:-72582761 | MS.gene33993:CDS |
| TCACCGGACGGCGAGGAGTT+TGG | 0.361360 | 1.2:-72589216 | MS.gene33993:CDS |
| GATCCATTGGTGGTAATGTT+TGG | 0.407930 | 1.2:-72582769 | MS.gene33993:CDS |
| TCCTGCCATTGAGGATGTCC+AGG | 0.411710 | 1.2:-72582645 | MS.gene33993:CDS |
| TGTTGATTCAAATGAAATCA+AGG | 0.432271 | 1.2:-72582720 | MS.gene33993:CDS |
| TGAGGATGTCCAGGTCTTCA+AGG | 0.437020 | 1.2:-72582636 | MS.gene33993:CDS |
| TAGAAAACCTGGGAATTCCT+TGG | 0.438964 | 1.2:+72589166 | None:intergenic |
| GACCTGACCGTACTCCTTGA+AGG | 0.446533 | 1.2:+72589248 | None:intergenic |
| AGCAACTCCGTCAACCTTCA+AGG | 0.460651 | 1.2:-72589262 | MS.gene33993:CDS |
| AGAAAACCTGGGAATTCCTT+GGG | 0.474456 | 1.2:+72589167 | None:intergenic |
| AGGTCAAGTTGAGCATCGTT+TGG | 0.477044 | 1.2:+72589192 | None:intergenic |
| GGACCAAACTCCTCGCCGTC+CGG | 0.492800 | 1.2:+72589213 | None:intergenic |
| TCATTTGAATCAACAATGGA+TGG | 0.494781 | 1.2:+72582728 | None:intergenic |
| TCTATTTAAGTCTGATACAA+TGG | 0.496626 | 1.2:-72582561 | MS.gene33993:CDS |
| ACCTCACCCAAGGAATTCCC+AGG | 0.499024 | 1.2:-72589173 | MS.gene33993:intron |
| CCGTACTCCTTGAAGGTTGA+CGG | 0.504142 | 1.2:+72589255 | None:intergenic |
| GTGATATTAGAAAACTTAAG+TGG | 0.517430 | 1.2:+72582821 | None:intergenic |
| ACCTGGACATCCTCAATGGC+AGG | 0.518519 | 1.2:+72582644 | None:intergenic |
| ACTCAATGCCTTGGATCCAT+TGG | 0.518618 | 1.2:-72582782 | MS.gene33993:CDS |
| AACCTTCAAGGAGTACGGTC+AGG | 0.537192 | 1.2:-72589250 | MS.gene33993:CDS |
| ACCTGGGAATTCCTTGGGTG+AGG | 0.537872 | 1.2:+72589172 | None:intergenic |
| CAATGCCTTGGATCCATTGG+TGG | 0.542939 | 1.2:-72582779 | MS.gene33993:CDS |
| ATACCAAACATTACCACCAA+TGG | 0.547916 | 1.2:+72582766 | None:intergenic |
| GAAGACCTGGACATCCTCAA+TGG | 0.556353 | 1.2:+72582640 | None:intergenic |
| TGAAGCTTAATCGTGGGACA+TGG | 0.559854 | 1.2:-72582595 | MS.gene33993:CDS |
| GCAAGTGTGACTCAATGCCT+TGG | 0.560708 | 1.2:-72582791 | MS.gene33993:CDS |
| TCAGGTCATTGAGGCTTCAC+CGG | 0.564970 | 1.2:-72589232 | MS.gene33993:CDS |
| GATTTCATTTGAATCAACAA+TGG | 0.567401 | 1.2:+72582724 | None:intergenic |
| AGCCTGAAACCTTGAAGACC+TGG | 0.571564 | 1.2:+72582627 | None:intergenic |
| CCGTCAACCTTCAAGGAGTA+CGG | 0.572133 | 1.2:-72589255 | MS.gene33993:CDS |
| TTATGTGCCTCCTGCCATTG+AGG | 0.572418 | 1.2:-72582654 | MS.gene33993:CDS |
| GTCATTGAGGCTTCACCGGA+CGG | 0.573850 | 1.2:-72589228 | MS.gene33993:CDS |
| AATCGTGGGACATGGCATGC+GGG | 0.581050 | 1.2:-72582587 | MS.gene33993:CDS |
| AATTTCTGAAGCTTAATCGT+GGG | 0.591063 | 1.2:-72582601 | MS.gene33993:CDS |
| CATTACCACCAATGGATCCA+AGG | 0.593498 | 1.2:+72582774 | None:intergenic |
| TGATATTAGAAAACTTAAGT+GGG | 0.595478 | 1.2:+72582822 | None:intergenic |
| ATCATAAATGGAGAGCGTAA+AGG | 0.598064 | 1.2:-72589307 | None:intergenic |
| TAATGTTTGGTATCTTGGAG+TGG | 0.602976 | 1.2:-72582756 | MS.gene33993:CDS |
| AAATTTCTGAAGCTTAATCG+TGG | 0.608012 | 1.2:-72582602 | MS.gene33993:CDS |
| TAATCGTGGGACATGGCATG+CGG | 0.608942 | 1.2:-72582588 | MS.gene33993:CDS |
| ATTGTATCAGACTTAAATAG+AGG | 0.622516 | 1.2:+72582563 | None:intergenic |
| GCTCAACTTGACCTCACCCA+AGG | 0.622952 | 1.2:-72589183 | MS.gene33993:CDS |
| AAATGAAATCAAGGATGACA+CGG | 0.629245 | 1.2:-72582711 | MS.gene33993:CDS |
| TGAGGCTTCACCGGACGGCG+AGG | 0.633096 | 1.2:-72589223 | MS.gene33993:CDS |
| GTGCTTCTTAAAGTTGTGTG+TGG | 0.641404 | 1.2:+72582341 | None:intergenic |
| CACAACTTTAAGAAGCACAA+TGG | 0.641416 | 1.2:-72582336 | MS.gene33993:CDS |
| AATGAAATCAAGGATGACAC+GGG | 0.656842 | 1.2:-72582710 | MS.gene33993:CDS |
| GGAGTACGGTCAGGTCATTG+AGG | 0.671075 | 1.2:-72589241 | MS.gene33993:CDS |
| TGGACATCCTCAATGGCAGG+AGG | 0.702078 | 1.2:+72582647 | None:intergenic |
| ACAGTCGTGAAATCGCGCAG+CGG | 0.734475 | 1.2:-72582683 | MS.gene33993:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 72582311 | 72589322 | 72582311 | ID=MS.gene33993 |
| chr1.2 | mRNA | 72582311 | 72589322 | 72582311 | ID=MS.gene33993.t1;Parent=MS.gene33993 |
| chr1.2 | exon | 72589174 | 72589322 | 72589174 | ID=MS.gene33993.t1.exon1;Parent=MS.gene33993.t1 |
| chr1.2 | CDS | 72589174 | 72589322 | 72589174 | ID=cds.MS.gene33993.t1;Parent=MS.gene33993.t1 |
| chr1.2 | exon | 72582529 | 72582871 | 72582529 | ID=MS.gene33993.t1.exon2;Parent=MS.gene33993.t1 |
| chr1.2 | CDS | 72582529 | 72582871 | 72582529 | ID=cds.MS.gene33993.t1;Parent=MS.gene33993.t1 |
| chr1.2 | exon | 72582311 | 72582376 | 72582311 | ID=MS.gene33993.t1.exon3;Parent=MS.gene33993.t1 |
| chr1.2 | CDS | 72582311 | 72582376 | 72582311 | ID=cds.MS.gene33993.t1;Parent=MS.gene33993.t1 |
| Gene Sequence |
| Protein sequence |
