Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62677.t1 | AET05445.2 | 100 | 167 | 0 | 0 | 1 | 167 | 1 | 167 | 9.80E-90 | 339.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62677.t1 | P30708 | 74.7 | 166 | 40 | 1 | 1 | 164 | 1 | 166 | 2.4e-68 | 259.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62677.t1 | G7LBF8 | 100.0 | 167 | 0 | 0 | 1 | 167 | 1 | 167 | 7.1e-90 | 339.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene62677 | 0.885323 | 8.98E-72 | -1.69E-46 |
| MS.gene049197 | MS.gene62677 | 0.815807 | 7.65E-52 | -1.69E-46 |
| MS.gene04919 | MS.gene62677 | 0.869322 | 3.38E-66 | -1.69E-46 |
| MS.gene04920 | MS.gene62677 | 0.887933 | 9.23E-73 | -1.69E-46 |
| MS.gene049254 | MS.gene62677 | 0.831559 | 1.56E-55 | -1.69E-46 |
| MS.gene049283 | MS.gene62677 | 0.878476 | 2.72E-69 | -1.69E-46 |
| MS.gene049419 | MS.gene62677 | -0.825752 | 3.95E-54 | -1.69E-46 |
| MS.gene049499 | MS.gene62677 | 0.876229 | 1.65E-68 | -1.69E-46 |
| MS.gene049652 | MS.gene62677 | 0.84749 | 1.12E-59 | -1.69E-46 |
| MS.gene049680 | MS.gene62677 | 0.87649 | 1.34E-68 | -1.69E-46 |
| MS.gene049983 | MS.gene62677 | 0.841797 | 3.82E-58 | -1.69E-46 |
| MS.gene04998 | MS.gene62677 | 0.803508 | 3.37E-49 | -1.69E-46 |
| MS.gene05029 | MS.gene62677 | 0.821483 | 3.94E-53 | -1.69E-46 |
| MS.gene050559 | MS.gene62677 | 0.817517 | 3.16E-52 | -1.69E-46 |
| MS.gene050561 | MS.gene62677 | 0.804861 | 1.76E-49 | -1.69E-46 |
| MS.gene050562 | MS.gene62677 | 0.807609 | 4.65E-50 | -1.69E-46 |
| MS.gene050646 | MS.gene62677 | 0.818473 | 1.92E-52 | -1.69E-46 |
| MS.gene050752 | MS.gene62677 | 0.806095 | 9.71E-50 | -1.69E-46 |
| MS.gene050945 | MS.gene62677 | 0.849662 | 2.80E-60 | -1.69E-46 |
| MS.gene050955 | MS.gene62677 | 0.81339 | 2.62E-51 | -1.69E-46 |
| MS.gene051003 | MS.gene62677 | 0.835356 | 1.76E-56 | -1.69E-46 |
| MS.gene051019 | MS.gene62677 | 0.83059 | 2.70E-55 | -1.69E-46 |
| MS.gene051043 | MS.gene62677 | 0.808943 | 2.42E-50 | -1.69E-46 |
| MS.gene051154 | MS.gene62677 | 0.835637 | 1.49E-56 | -1.69E-46 |
| MS.gene051234 | MS.gene62677 | 0.817216 | 3.70E-52 | -1.69E-46 |
| MS.gene051332 | MS.gene62677 | 0.804521 | 2.08E-49 | -1.69E-46 |
| MS.gene051343 | MS.gene62677 | 0.825155 | 5.47E-54 | -1.69E-46 |
| MS.gene051597 | MS.gene62677 | 0.805896 | 1.07E-49 | -1.69E-46 |
| MS.gene051637 | MS.gene62677 | 0.842842 | 2.02E-58 | -1.69E-46 |
| MS.gene051676 | MS.gene62677 | 0.813609 | 2.35E-51 | -1.69E-46 |
| MS.gene051699 | MS.gene62677 | 0.890344 | 1.07E-73 | -1.69E-46 |
| MS.gene051744 | MS.gene62677 | 0.81832 | 2.09E-52 | -1.69E-46 |
| MS.gene051832 | MS.gene62677 | 0.861233 | 1.18E-63 | -1.69E-46 |
| MS.gene051848 | MS.gene62677 | -0.814996 | 1.16E-51 | -1.69E-46 |
| MS.gene051959 | MS.gene62677 | 0.828924 | 6.86E-55 | -1.69E-46 |
| MS.gene051960 | MS.gene62677 | 0.825335 | 4.96E-54 | -1.69E-46 |
| MS.gene051975 | MS.gene62677 | 0.859116 | 5.17E-63 | -1.69E-46 |
| MS.gene052072 | MS.gene62677 | 0.826327 | 2.88E-54 | -1.69E-46 |
| MS.gene052118 | MS.gene62677 | 0.817544 | 3.12E-52 | -1.69E-46 |
| MS.gene052296 | MS.gene62677 | 0.807269 | 5.49E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene62677 | 0.801822 | 7.51E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene62677 | 0.808609 | 2.85E-50 | -1.69E-46 |
| MS.gene052518 | MS.gene62677 | 0.817393 | 3.38E-52 | -1.69E-46 |
| MS.gene052533 | MS.gene62677 | 0.858975 | 5.69E-63 | -1.69E-46 |
| MS.gene052559 | MS.gene62677 | 0.820155 | 7.96E-53 | -1.69E-46 |
| MS.gene052685 | MS.gene62677 | 0.858176 | 9.86E-63 | -1.69E-46 |
| MS.gene052717 | MS.gene62677 | 0.800433 | 1.44E-48 | -1.69E-46 |
| MS.gene052793 | MS.gene62677 | 0.85274 | 3.78E-61 | -1.69E-46 |
| MS.gene052855 | MS.gene62677 | 0.804196 | 2.43E-49 | -1.69E-46 |
| MS.gene052888 | MS.gene62677 | 0.812265 | 4.63E-51 | -1.69E-46 |
| MS.gene052889 | MS.gene62677 | 0.831298 | 1.81E-55 | -1.69E-46 |
| MS.gene052890 | MS.gene62677 | 0.803022 | 4.25E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene62677 | 0.810201 | 1.30E-50 | -1.69E-46 |
| MS.gene053045 | MS.gene62677 | 0.810619 | 1.05E-50 | -1.69E-46 |
| MS.gene053094 | MS.gene62677 | 0.84508 | 5.08E-59 | -1.69E-46 |
| MS.gene053228 | MS.gene62677 | 0.847776 | 9.34E-60 | -1.69E-46 |
| MS.gene053272 | MS.gene62677 | -0.830263 | 3.24E-55 | -1.69E-46 |
| MS.gene053295 | MS.gene62677 | 0.824458 | 7.98E-54 | -1.69E-46 |
| MS.gene053298 | MS.gene62677 | 0.841288 | 5.20E-58 | -1.69E-46 |
| MS.gene053340 | MS.gene62677 | 0.811277 | 7.59E-51 | -1.69E-46 |
| MS.gene053401 | MS.gene62677 | 0.807275 | 5.47E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene62677 | 0.806297 | 8.81E-50 | -1.69E-46 |
| MS.gene053575 | MS.gene62677 | 0.810103 | 1.36E-50 | -1.69E-46 |
| MS.gene053879 | MS.gene62677 | 0.846564 | 2.01E-59 | -1.69E-46 |
| MS.gene053931 | MS.gene62677 | 0.817285 | 3.57E-52 | -1.69E-46 |
| MS.gene053963 | MS.gene62677 | 0.892357 | 1.71E-74 | -1.69E-46 |
| MS.gene054004 | MS.gene62677 | 0.803823 | 2.90E-49 | -1.69E-46 |
| MS.gene05410 | MS.gene62677 | 0.800324 | 1.52E-48 | -1.69E-46 |
| MS.gene054222 | MS.gene62677 | 0.879533 | 1.15E-69 | -1.69E-46 |
| MS.gene054403 | MS.gene62677 | 0.813336 | 2.70E-51 | -1.69E-46 |
| MS.gene054413 | MS.gene62677 | 0.813449 | 2.55E-51 | -1.69E-46 |
| MS.gene054576 | MS.gene62677 | 0.813498 | 2.48E-51 | -1.69E-46 |
| MS.gene05458 | MS.gene62677 | 0.802216 | 6.23E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene62677 | 0.803215 | 3.88E-49 | -1.69E-46 |
| MS.gene054684 | MS.gene62677 | 0.811145 | 8.11E-51 | -1.69E-46 |
| MS.gene05469 | MS.gene62677 | 0.835741 | 1.41E-56 | -1.69E-46 |
| MS.gene054892 | MS.gene62677 | 0.88253 | 9.64E-71 | -1.69E-46 |
| MS.gene054893 | MS.gene62677 | 0.888678 | 4.77E-73 | -1.69E-46 |
| MS.gene054957 | MS.gene62677 | 0.864237 | 1.41E-64 | -1.69E-46 |
| MS.gene054960 | MS.gene62677 | 0.806548 | 7.80E-50 | -1.69E-46 |
| MS.gene055141 | MS.gene62677 | 0.800687 | 1.28E-48 | -1.69E-46 |
| MS.gene055147 | MS.gene62677 | 0.849588 | 2.94E-60 | -1.69E-46 |
| MS.gene055148 | MS.gene62677 | 0.842947 | 1.89E-58 | -1.69E-46 |
| MS.gene055149 | MS.gene62677 | 0.82423 | 9.03E-54 | -1.69E-46 |
| MS.gene055150 | MS.gene62677 | 0.89876 | 3.87E-77 | -1.69E-46 |
| MS.gene055226 | MS.gene62677 | 0.80251 | 5.42E-49 | -1.69E-46 |
| MS.gene055229 | MS.gene62677 | 0.817447 | 3.28E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene62677 | 0.801025 | 1.09E-48 | -1.69E-46 |
| MS.gene055555 | MS.gene62677 | 0.805182 | 1.51E-49 | -1.69E-46 |
| MS.gene055586 | MS.gene62677 | 0.800763 | 1.24E-48 | -1.69E-46 |
| MS.gene055801 | MS.gene62677 | 0.845651 | 3.56E-59 | -1.69E-46 |
| MS.gene055841 | MS.gene62677 | 0.816093 | 6.60E-52 | -1.69E-46 |
| MS.gene056071 | MS.gene62677 | 0.811518 | 6.73E-51 | -1.69E-46 |
| MS.gene056082 | MS.gene62677 | 0.812256 | 4.65E-51 | -1.69E-46 |
| MS.gene056100 | MS.gene62677 | 0.804527 | 2.07E-49 | -1.69E-46 |
| MS.gene05624 | MS.gene62677 | 0.867249 | 1.57E-65 | -1.69E-46 |
| MS.gene05636 | MS.gene62677 | 0.801514 | 8.69E-49 | -1.69E-46 |
| MS.gene056677 | MS.gene62677 | 0.854574 | 1.12E-61 | -1.69E-46 |
| MS.gene056699 | MS.gene62677 | 0.868102 | 8.38E-66 | -1.69E-46 |
| MS.gene056776 | MS.gene62677 | 0.80381 | 2.92E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene62677 | 0.850511 | 1.62E-60 | -1.69E-46 |
| MS.gene05689 | MS.gene62677 | 0.820099 | 8.20E-53 | -1.69E-46 |
| MS.gene05690 | MS.gene62677 | 0.813556 | 2.41E-51 | -1.69E-46 |
| MS.gene056986 | MS.gene62677 | 0.872986 | 2.09E-67 | -1.69E-46 |
| MS.gene057102 | MS.gene62677 | 0.904897 | 7.59E-80 | -1.69E-46 |
| MS.gene057155 | MS.gene62677 | 0.815254 | 1.02E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene62677 | 0.848198 | 7.14E-60 | -1.69E-46 |
| MS.gene057622 | MS.gene62677 | 0.8487 | 5.19E-60 | -1.69E-46 |
| MS.gene057890 | MS.gene62677 | -0.82965 | 4.57E-55 | -1.69E-46 |
| MS.gene057893 | MS.gene62677 | -0.831522 | 1.59E-55 | -1.69E-46 |
| MS.gene057999 | MS.gene62677 | 0.835375 | 1.74E-56 | -1.69E-46 |
| MS.gene058008 | MS.gene62677 | 0.840631 | 7.74E-58 | -1.69E-46 |
| MS.gene058009 | MS.gene62677 | 0.802855 | 4.60E-49 | -1.69E-46 |
| MS.gene058095 | MS.gene62677 | 0.849837 | 2.50E-60 | -1.69E-46 |
| MS.gene058159 | MS.gene62677 | 0.889378 | 2.56E-73 | -1.69E-46 |
| MS.gene058279 | MS.gene62677 | 0.844893 | 5.70E-59 | -1.69E-46 |
| MS.gene058592 | MS.gene62677 | 0.908736 | 1.23E-81 | -1.69E-46 |
| MS.gene058635 | MS.gene62677 | 0.813702 | 2.24E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene62677 | 0.852637 | 4.05E-61 | -1.69E-46 |
| MS.gene058783 | MS.gene62677 | 0.839024 | 2.03E-57 | -1.69E-46 |
| MS.gene05878 | MS.gene62677 | 0.878689 | 2.29E-69 | -1.69E-46 |
| MS.gene059051 | MS.gene62677 | 0.836913 | 7.08E-57 | -1.69E-46 |
| MS.gene059146 | MS.gene62677 | 0.851229 | 1.02E-60 | -1.69E-46 |
| MS.gene059170 | MS.gene62677 | 0.839854 | 1.24E-57 | -1.69E-46 |
| MS.gene059227 | MS.gene62677 | 0.80974 | 1.63E-50 | -1.69E-46 |
| MS.gene059436 | MS.gene62677 | 0.82006 | 8.37E-53 | -1.69E-46 |
| MS.gene05952 | MS.gene62677 | 0.917938 | 2.88E-86 | -1.69E-46 |
| MS.gene05956 | MS.gene62677 | 0.85552 | 5.97E-62 | -1.69E-46 |
| MS.gene059774 | MS.gene62677 | 0.820791 | 5.69E-53 | -1.69E-46 |
| MS.gene05985 | MS.gene62677 | -0.918048 | 2.52E-86 | -1.69E-46 |
| MS.gene059901 | MS.gene62677 | -0.880757 | 4.22E-70 | -1.69E-46 |
| MS.gene059935 | MS.gene62677 | 0.818479 | 1.92E-52 | -1.69E-46 |
| MS.gene060012 | MS.gene62677 | 0.832404 | 9.64E-56 | -1.69E-46 |
| MS.gene06011 | MS.gene62677 | 0.823793 | 1.14E-53 | -1.69E-46 |
| MS.gene06018 | MS.gene62677 | 0.802324 | 5.92E-49 | -1.69E-46 |
| MS.gene06023 | MS.gene62677 | 0.846361 | 2.28E-59 | -1.69E-46 |
| MS.gene060244 | MS.gene62677 | 0.833849 | 4.21E-56 | -1.69E-46 |
| MS.gene060259 | MS.gene62677 | 0.82741 | 1.59E-54 | -1.69E-46 |
| MS.gene060338 | MS.gene62677 | 0.848511 | 5.85E-60 | -1.69E-46 |
| MS.gene060365 | MS.gene62677 | 0.838582 | 2.64E-57 | -1.69E-46 |
| MS.gene060412 | MS.gene62677 | 0.844716 | 6.36E-59 | -1.69E-46 |
| MS.gene060413 | MS.gene62677 | 0.802225 | 6.20E-49 | -1.69E-46 |
| MS.gene060512 | MS.gene62677 | 0.835752 | 1.40E-56 | -1.69E-46 |
| MS.gene060522 | MS.gene62677 | 0.817963 | 2.51E-52 | -1.69E-46 |
| MS.gene060527 | MS.gene62677 | 0.823529 | 1.32E-53 | -1.69E-46 |
| MS.gene060697 | MS.gene62677 | 0.8144 | 1.57E-51 | -1.69E-46 |
| MS.gene060716 | MS.gene62677 | 0.809139 | 2.19E-50 | -1.69E-46 |
| MS.gene060763 | MS.gene62677 | 0.82136 | 4.21E-53 | -1.69E-46 |
| MS.gene060777 | MS.gene62677 | 0.816817 | 4.55E-52 | -1.69E-46 |
| MS.gene060840 | MS.gene62677 | 0.835772 | 1.38E-56 | -1.69E-46 |
| MS.gene060932 | MS.gene62677 | 0.809259 | 2.07E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene62677 | 0.844892 | 5.71E-59 | -1.69E-46 |
| MS.gene060935 | MS.gene62677 | 0.870721 | 1.18E-66 | -1.69E-46 |
| MS.gene060937 | MS.gene62677 | 0.883722 | 3.53E-71 | -1.69E-46 |
| MS.gene061047 | MS.gene62677 | 0.81927 | 1.27E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene62677 | 0.8623 | 5.59E-64 | -1.69E-46 |
| MS.gene061160 | MS.gene62677 | 0.840141 | 1.04E-57 | -1.69E-46 |
| MS.gene061188 | MS.gene62677 | 0.871987 | 4.49E-67 | -1.69E-46 |
| MS.gene061243 | MS.gene62677 | 0.833512 | 5.11E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62677.t1 | MTR_8g105630 | 100.000 | 167 | 0 | 0 | 1 | 167 | 1 | 167 | 2.27e-122 | 342 |
| MS.gene62677.t1 | MTR_8g098410 | 72.727 | 165 | 44 | 1 | 1 | 164 | 67 | 231 | 1.77e-87 | 256 |
| MS.gene62677.t1 | MTR_8g098410 | 71.856 | 167 | 46 | 1 | 1 | 166 | 1 | 167 | 2.86e-87 | 253 |
| MS.gene62677.t1 | MTR_1g014210 | 66.049 | 162 | 55 | 0 | 5 | 166 | 75 | 236 | 8.81e-79 | 234 |
| MS.gene62677.t1 | MTR_8g098400 | 65.823 | 158 | 54 | 0 | 7 | 164 | 12 | 169 | 2.23e-75 | 223 |
| MS.gene62677.t1 | MTR_7g094600 | 61.250 | 160 | 62 | 0 | 5 | 164 | 8 | 167 | 8.41e-73 | 216 |
| MS.gene62677.t1 | MTR_8g098400 | 65.563 | 151 | 52 | 0 | 7 | 157 | 12 | 162 | 5.29e-72 | 214 |
| MS.gene62677.t1 | MTR_5g010340 | 63.816 | 152 | 54 | 1 | 7 | 157 | 10 | 161 | 1.20e-68 | 207 |
| MS.gene62677.t1 | MTR_1g072570 | 57.233 | 159 | 67 | 1 | 8 | 165 | 11 | 169 | 2.22e-64 | 195 |
| MS.gene62677.t1 | MTR_8g098400 | 63.934 | 122 | 44 | 0 | 43 | 164 | 1 | 122 | 3.89e-54 | 167 |
| MS.gene62677.t1 | MTR_7g094600 | 60.163 | 123 | 49 | 0 | 42 | 164 | 7 | 129 | 1.86e-53 | 166 |
| MS.gene62677.t1 | MTR_8g098410 | 72.816 | 103 | 27 | 1 | 1 | 102 | 67 | 169 | 4.38e-49 | 157 |
| MS.gene62677.t1 | MTR_8g098400 | 63.542 | 96 | 35 | 0 | 7 | 102 | 12 | 107 | 3.29e-40 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62677.t1 | AT2G31570 | 73.780 | 164 | 43 | 0 | 1 | 164 | 1 | 164 | 1.86e-89 | 258 |
| MS.gene62677.t1 | AT2G43350 | 71.687 | 166 | 47 | 0 | 2 | 167 | 41 | 206 | 3.06e-87 | 254 |
| MS.gene62677.t1 | AT2G43350 | 71.687 | 166 | 47 | 0 | 2 | 167 | 41 | 206 | 3.06e-87 | 254 |
| MS.gene62677.t1 | AT4G11600 | 71.166 | 163 | 47 | 0 | 2 | 164 | 66 | 228 | 1.01e-85 | 251 |
| MS.gene62677.t1 | AT4G31870 | 69.375 | 160 | 49 | 0 | 7 | 166 | 74 | 233 | 9.79e-83 | 244 |
| MS.gene62677.t1 | AT2G25080 | 64.198 | 162 | 58 | 0 | 5 | 166 | 75 | 236 | 3.28e-78 | 233 |
| MS.gene62677.t1 | AT1G63460 | 60.479 | 167 | 66 | 0 | 1 | 167 | 1 | 167 | 1.70e-76 | 226 |
| MS.gene62677.t1 | AT3G63080 | 66.026 | 156 | 53 | 0 | 9 | 164 | 14 | 169 | 2.11e-74 | 221 |
| MS.gene62677.t1 | AT2G48150 | 63.354 | 161 | 59 | 0 | 7 | 167 | 10 | 170 | 5.64e-74 | 219 |
| MS.gene62677.t1 | AT2G48150 | 64.583 | 144 | 51 | 0 | 24 | 167 | 1 | 144 | 3.33e-66 | 199 |
| MS.gene62677.t1 | AT4G31870 | 63.333 | 60 | 22 | 0 | 7 | 66 | 74 | 133 | 1.36e-21 | 86.3 |
Find 33 sgRNAs with CRISPR-Local
Find 125 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATCAGAAAGGAGGAATATT+TGG | 0.230959 | 4.2:+1388849 | MS.gene62677:CDS |
| GTCTCTAGACTTTGAAATCT+TGG | 0.241499 | 4.2:+1388438 | MS.gene62677:intron |
| GACCTTGATCCTTATACTTC+TGG | 0.294629 | 4.2:-1388320 | None:intergenic |
| CTGGTTCCTGTCCACGAAAC+TGG | 0.374855 | 4.2:-1388474 | None:intergenic |
| AGTGAAATCGTAGATGGATT+TGG | 0.407313 | 4.2:-1386877 | None:intergenic |
| TGTTCTGTACCAGAAGTATA+AGG | 0.413755 | 4.2:+1388311 | MS.gene62677:CDS |
| TATTATAGGTTGAAGTCAAT+GGG | 0.436019 | 4.2:+1388793 | MS.gene62677:intron |
| TTATTATAGGTTGAAGTCAA+TGG | 0.438351 | 4.2:+1388792 | MS.gene62677:intron |
| CTTGACAGTGAAATCGTAGA+TGG | 0.450016 | 4.2:-1386883 | None:intergenic |
| GTAATCACCTTATCAAAGAC+TGG | 0.452445 | 4.2:-1388550 | None:intergenic |
| TGAATTTCTTCACTAGATCC+TGG | 0.461518 | 4.2:-1388493 | None:intergenic |
| TGAATTCCCAGTCTTTGATA+AGG | 0.466937 | 4.2:+1388543 | MS.gene62677:CDS |
| ATGCAACCAGTTTCGTGGAC+AGG | 0.495715 | 4.2:+1388468 | MS.gene62677:CDS |
| TTTCCATGCAACCAGTTTCG+TGG | 0.506265 | 4.2:+1388463 | MS.gene62677:CDS |
| AAGTTCTTAGTAAACAAAGA+AGG | 0.522522 | 4.2:+1388897 | MS.gene62677:CDS |
| TACCAGAAGTATAAGGATCA+AGG | 0.527051 | 4.2:+1388318 | MS.gene62677:CDS |
| CAGTTTCGTGGACAGGAACC+AGG | 0.539854 | 4.2:+1388475 | MS.gene62677:CDS |
| TTCAGAATGTTGTTTGCACA+AGG | 0.545993 | 4.2:+1388512 | MS.gene62677:CDS |
| CTTAGTAAACAAAGAAGGGA+AGG | 0.552208 | 4.2:+1388902 | MS.gene62677:CDS |
| ATACAGAGGAAAGGTTCTGC+TGG | 0.554671 | 4.2:+1387076 | MS.gene62677:CDS |
| CATCTACGATTTCACTGTCA+AGG | 0.560391 | 4.2:+1386884 | MS.gene62677:CDS |
| TGTCCACGAAACTGGTTGCA+TGG | 0.566923 | 4.2:-1388466 | None:intergenic |
| GAAATTAAGTTACCATTGTG+AGG | 0.576973 | 4.2:-1387111 | None:intergenic |
| AGTTCTTAGTAAACAAAGAA+GGG | 0.577714 | 4.2:+1388898 | MS.gene62677:CDS |
| AAACAAAGAAGGGAAGGTTG+TGG | 0.578941 | 4.2:+1388908 | MS.gene62677:CDS |
| AAAGGAGGAATATTTGGAGA+TGG | 0.590133 | 4.2:+1388855 | MS.gene62677:CDS |
| TAATCACCTTATCAAAGACT+GGG | 0.624432 | 4.2:-1388549 | None:intergenic |
| TCTGAGTCAATACAGAGGAA+AGG | 0.631545 | 4.2:+1387067 | MS.gene62677:CDS |
| CTATAATTGCAGGATATCAG+TGG | 0.642189 | 4.2:+1387032 | MS.gene62677:intron |
| TATTTGGAGATGGTATCAAG+TGG | 0.648183 | 4.2:+1388865 | MS.gene62677:CDS |
| CACCGCTCCTCTGAAAATCG+AGG | 0.662646 | 4.2:+1388947 | MS.gene62677:CDS |
| TTGTCAATGTTGCCTCACAA+TGG | 0.714648 | 4.2:+1387099 | MS.gene62677:CDS |
| GTGAGTCTGAGTCAATACAG+AGG | 0.737687 | 4.2:+1387062 | MS.gene62677:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATATATATGAACAAA+CGG | - | chr4.2:1387297-1387316 | None:intergenic | 10.0% |
| !!! | ATGATTGATTATAATTTTTT+TGG | + | chr4.2:1387396-1387415 | MS.gene62677:intron | 10.0% |
| !!! | TTAAAAAATTGTTTTTTGAT+GGG | + | chr4.2:1386944-1386963 | MS.gene62677:intron | 10.0% |
| !!! | TTTAAAAAATTGTTTTTTGA+TGG | + | chr4.2:1386943-1386962 | MS.gene62677:intron | 10.0% |
| !! | AAAGAATTTACAATAAATCA+TGG | - | chr4.2:1388575-1388594 | None:intergenic | 15.0% |
| !! | ACAATTATAGTTATAGTTAT+AGG | - | chr4.2:1388235-1388254 | None:intergenic | 15.0% |
| !! | ATTGAAAAAACATCAAAAAA+TGG | - | chr4.2:1386912-1386931 | None:intergenic | 15.0% |
| !! | GTATATGAATTAAATCATAA+AGG | - | chr4.2:1387273-1387292 | None:intergenic | 15.0% |
| !! | TAAATTAAAATATGTAGATC+AGG | - | chr4.2:1387181-1387200 | None:intergenic | 15.0% |
| !!! | AAAAAAAAGCATATACAATT+TGG | - | chr4.2:1388677-1388696 | None:intergenic | 15.0% |
| !!! | ACTTAATTTCATTCATTTTT+TGG | + | chr4.2:1387124-1387143 | MS.gene62677:intron | 15.0% |
| !!! | TTGTATTTTTGTTTTGTTTT+TGG | + | chr4.2:1387978-1387997 | MS.gene62677:intron | 15.0% |
| !! | AAAATGATTTCCATATAAAC+TGG | - | chr4.2:1388761-1388780 | None:intergenic | 20.0% |
| !! | AATATATATGCCAGTTTATA+TGG | + | chr4.2:1388748-1388767 | MS.gene62677:intron | 20.0% |
| !! | TTTGTAACTTATTTGACATT+TGG | + | chr4.2:1388592-1388611 | MS.gene62677:intron | 20.0% |
| !!! | AAGTTATTAAGGAAATTTTG+CGG | + | chr4.2:1388020-1388039 | MS.gene62677:intron | 20.0% |
| !!! | ATTTTTTTTGTTTTTGTCCT+TGG | - | chr4.2:1388357-1388376 | None:intergenic | 20.0% |
| !!! | CATTTTTGTGCTTATATTTT+AGG | + | chr4.2:1388977-1388996 | MS.gene62677:intron | 20.0% |
| !!! | GATTTTTATTCAGTAGTTAA+TGG | + | chr4.2:1387754-1387773 | MS.gene62677:intron | 20.0% |
| !!! | TATTTTATGCTGTTTTGTTT+TGG | + | chr4.2:1387902-1387921 | MS.gene62677:intron | 20.0% |
| ! | AAGTTCTTAGTAAACAAAGA+AGG | + | chr4.2:1388897-1388916 | MS.gene62677:CDS | 25.0% |
| ! | AGTTATAGGACAGAATAAAA+TGG | - | chr4.2:1388221-1388240 | None:intergenic | 25.0% |
| ! | AGTTCTTAGTAAACAAAGAA+GGG | + | chr4.2:1388898-1388917 | MS.gene62677:CDS | 25.0% |
| ! | ATTAATCTTTCTCCATTAGT+AGG | + | chr4.2:1387692-1387711 | MS.gene62677:intron | 25.0% |
| ! | TGAATTAAATCATAAAGGAC+TGG | - | chr4.2:1387268-1387287 | None:intergenic | 25.0% |
| ! | TGACATGAGATTTAAATCAA+TGG | + | chr4.2:1387796-1387815 | MS.gene62677:intron | 25.0% |
| ! | TTAGAATCTGAACAAATAAC+AGG | - | chr4.2:1387721-1387740 | None:intergenic | 25.0% |
| !! | AGTCAAACATTTTTAACGAT+AGG | - | chr4.2:1387345-1387364 | None:intergenic | 25.0% |
| !! | TATATGTTTCTTTTTGCAGA+AGG | + | chr4.2:1389044-1389063 | MS.gene62677:intron | 25.0% |
| !! | TATTATAGGTTGAAGTCAAT+GGG | + | chr4.2:1388793-1388812 | MS.gene62677:intron | 25.0% |
| !! | TTATTATAGGTTGAAGTCAA+TGG | + | chr4.2:1388792-1388811 | MS.gene62677:intron | 25.0% |
| AAAGCATATACAATTTGGCT+AGG | - | chr4.2:1388672-1388691 | None:intergenic | 30.0% | |
| AAGCATATACAATTTGGCTA+GGG | - | chr4.2:1388671-1388690 | None:intergenic | 30.0% | |
| AATCACACAACATAATCCAT+AGG | - | chr4.2:1387529-1387548 | None:intergenic | 30.0% | |
| ATTCTAATTCATTGTTGCAG+CGG | + | chr4.2:1388258-1388277 | MS.gene62677:intron | 30.0% | |
| CATGAGATTTAAATCAATGG+TGG | + | chr4.2:1387799-1387818 | MS.gene62677:intron | 30.0% | |
| CGGCTTAGAAAAAGTTATTA+AGG | + | chr4.2:1388009-1388028 | MS.gene62677:intron | 30.0% | |
| GAAATTAAGTTACCATTGTG+AGG | - | chr4.2:1387114-1387133 | None:intergenic | 30.0% | |
| GACAGATCATAATTGATTGA+TGG | - | chr4.2:1387323-1387342 | None:intergenic | 30.0% | |
| GTGATATGTACTATAATTGC+AGG | + | chr4.2:1387022-1387041 | MS.gene62677:intron | 30.0% | |
| TAATCACCTTATCAAAGACT+GGG | - | chr4.2:1388552-1388571 | None:intergenic | 30.0% | |
| TCCTTCAAAAACTTGTAAAG+TGG | - | chr4.2:1388831-1388850 | None:intergenic | 30.0% | |
| TTGATGTTTGAGAAATGTCA+TGG | + | chr4.2:1387580-1387599 | MS.gene62677:intron | 30.0% | |
| ! | AAGAATACTTGCACAGTTTT+TGG | + | chr4.2:1387649-1387668 | MS.gene62677:intron | 30.0% |
| ! | ACCACTTTACAAGTTTTTGA+AGG | + | chr4.2:1388827-1388846 | MS.gene62677:CDS | 30.0% |
| ! | TTTTTGCAATGAATTCTGCA+AGG | + | chr4.2:1388710-1388729 | MS.gene62677:intron | 30.0% |
| !! | AAGTTTTTGAAGGATCAGAA+AGG | + | chr4.2:1388837-1388856 | MS.gene62677:CDS | 30.0% |
| !! | AATAACTTTTTCTAAGCCGA+AGG | - | chr4.2:1388008-1388027 | None:intergenic | 30.0% |
| !! | GATGGATTTGGATGAATTTT+CGG | - | chr4.2:1386868-1386887 | None:intergenic | 30.0% |
| !! | TTTTTGCGTTGTTTTGTTCT+TGG | + | chr4.2:1387935-1387954 | MS.gene62677:intron | 30.0% |
| !!! | ATTGTTTTTTGATGGGTCAA+TGG | + | chr4.2:1386951-1386970 | MS.gene62677:intron | 30.0% |
| !!! | TTTATGCTGTTTTGTTTTGG+TGG | + | chr4.2:1387905-1387924 | MS.gene62677:intron | 30.0% |
| !!! | TTTGATCTGTTTTTGTTGCT+TGG | + | chr4.2:1387865-1387884 | MS.gene62677:intron | 30.0% |
| AAAGGAGGAATATTTGGAGA+TGG | + | chr4.2:1388855-1388874 | MS.gene62677:CDS | 35.0% | |
| AATAACAGGTCACCTACTAA+TGG | - | chr4.2:1387707-1387726 | None:intergenic | 35.0% | |
| AATCATATGTGCGACTTTAG+AGG | - | chr4.2:1387382-1387401 | None:intergenic | 35.0% | |
| ACAAAACAATTACCCACGAA+GGG | - | chr4.2:1387962-1387981 | None:intergenic | 35.0% | |
| ATAGAATTGCGAAGTATAGC+TGG | + | chr4.2:1387501-1387520 | MS.gene62677:intron | 35.0% | |
| CTATAATTGCAGGATATCAG+TGG | + | chr4.2:1387032-1387051 | MS.gene62677:intron | 35.0% | |
| CTTAGTAAACAAAGAAGGGA+AGG | + | chr4.2:1388902-1388921 | MS.gene62677:CDS | 35.0% | |
| CTTCTTCAAAAAACAGTTGG+GGG | + | chr4.2:1388043-1388062 | MS.gene62677:intron | 35.0% | |
| GAGATTGGACTATCATATGT+GGG | + | chr4.2:1388067-1388086 | MS.gene62677:intron | 35.0% | |
| GATCAGAAAGGAGGAATATT+TGG | + | chr4.2:1388849-1388868 | MS.gene62677:CDS | 35.0% | |
| GCTTCTTCAAAAAACAGTTG+GGG | + | chr4.2:1388042-1388061 | MS.gene62677:intron | 35.0% | |
| GGCTTCTTCAAAAAACAGTT+GGG | + | chr4.2:1388041-1388060 | MS.gene62677:intron | 35.0% | |
| GTAATCACCTTATCAAAGAC+TGG | - | chr4.2:1388553-1388572 | None:intergenic | 35.0% | |
| TACAAAACAATTACCCACGA+AGG | - | chr4.2:1387963-1387982 | None:intergenic | 35.0% | |
| TATTTGGAGATGGTATCAAG+TGG | + | chr4.2:1388865-1388884 | MS.gene62677:CDS | 35.0% | |
| TGAATTCCCAGTCTTTGATA+AGG | + | chr4.2:1388543-1388562 | MS.gene62677:CDS | 35.0% | |
| TGAATTTCTTCACTAGATCC+TGG | - | chr4.2:1388496-1388515 | None:intergenic | 35.0% | |
| TGAGATTGGACTATCATATG+TGG | + | chr4.2:1388066-1388085 | MS.gene62677:intron | 35.0% | |
| TTACATCCTCTTGACTGTTT+GGG | + | chr4.2:1387822-1387841 | MS.gene62677:intron | 35.0% | |
| TTTGTCCTTGGTTAAAGTCA+CGG | - | chr4.2:1388345-1388364 | None:intergenic | 35.0% | |
| ! | AGGACATTGAGAAGCTTTTA+CGG | + | chr4.2:1389064-1389083 | MS.gene62677:CDS | 35.0% |
| ! | AGTGAAATCGTAGATGGATT+TGG | - | chr4.2:1386880-1386899 | None:intergenic | 35.0% |
| ! | TACCAGAAGTATAAGGATCA+AGG | + | chr4.2:1388318-1388337 | MS.gene62677:CDS | 35.0% |
| ! | TATCTAACCTCGATTTTCAG+AGG | - | chr4.2:1388957-1388976 | None:intergenic | 35.0% |
| ! | TGTTCTGTACCAGAAGTATA+AGG | + | chr4.2:1388311-1388330 | MS.gene62677:CDS | 35.0% |
| ! | TTCAGAATGTTGTTTGCACA+AGG | + | chr4.2:1388512-1388531 | MS.gene62677:CDS | 35.0% |
| !! | GCACAGTTTTTGGATTTATG+AGG | + | chr4.2:1387659-1387678 | MS.gene62677:intron | 35.0% |
| !! | GTCTCTAGACTTTGAAATCT+TGG | + | chr4.2:1388438-1388457 | MS.gene62677:intron | 35.0% |
| !! | TTGTGCTTATATTTTAGGCC+AGG | + | chr4.2:1388982-1389001 | MS.gene62677:intron | 35.0% |
| !! | TTTTTGAAGGATCAGAAAGG+AGG | + | chr4.2:1388840-1388859 | MS.gene62677:CDS | 35.0% |
| !!! | TCTGTTTTTGTTGCTTGGTT+TGG | + | chr4.2:1387870-1387889 | MS.gene62677:intron | 35.0% |
| !!! | TTTTGTTTTTGGCACACCTT+CGG | + | chr4.2:1387989-1388008 | MS.gene62677:intron | 35.0% |
| AAACAAAGAAGGGAAGGTTG+TGG | + | chr4.2:1388908-1388927 | MS.gene62677:CDS | 40.0% | |
| ATCTGACACTGTAAAGCCTT+AGG | + | chr4.2:1388104-1388123 | MS.gene62677:intron | 40.0% | |
| CAAAACAATTACCCACGAAG+GGG | - | chr4.2:1387961-1387980 | None:intergenic | 40.0% | |
| CAAAGCAACGACTTCTTTGT+TGG | + | chr4.2:1388135-1388154 | MS.gene62677:intron | 40.0% | |
| CAACAAAGAAGTCGTTGCTT+TGG | - | chr4.2:1388137-1388156 | None:intergenic | 40.0% | |
| CATCTACGATTTCACTGTCA+AGG | + | chr4.2:1386884-1386903 | MS.gene62677:CDS | 40.0% | |
| CGGCTTCTTCAAAAAACAGT+TGG | + | chr4.2:1388040-1388059 | MS.gene62677:intron | 40.0% | |
| CTTGACAGTGAAATCGTAGA+TGG | - | chr4.2:1386886-1386905 | None:intergenic | 40.0% | |
| GACCTTGATCCTTATACTTC+TGG | - | chr4.2:1388323-1388342 | None:intergenic | 40.0% | |
| GTTACATCCTCTTGACTGTT+TGG | + | chr4.2:1387821-1387840 | MS.gene62677:intron | 40.0% | |
| GTTTGGACTCTCGATATACT+TGG | + | chr4.2:1388639-1388658 | MS.gene62677:intron | 40.0% | |
| TCTGAGTCAATACAGAGGAA+AGG | + | chr4.2:1387067-1387086 | MS.gene62677:CDS | 40.0% | |
| TTGTCAATGTTGCCTCACAA+TGG | + | chr4.2:1387099-1387118 | MS.gene62677:CDS | 40.0% | |
| ! | TTGCTTTGGCTTAGATCCTA+AGG | - | chr4.2:1388123-1388142 | None:intergenic | 40.0% |
| !!! | GTTTTTGTTGCTTGGTTTGG+AGG | + | chr4.2:1387873-1387892 | MS.gene62677:intron | 40.0% |
| AAAACAGTTGGGGGTGAGAT+TGG | + | chr4.2:1388052-1388071 | MS.gene62677:intron | 45.0% | |
| AAGGTCCGTGACTTTAACCA+AGG | + | chr4.2:1388337-1388356 | MS.gene62677:intron | 45.0% | |
| ATACAGAGGAAAGGTTCTGC+TGG | + | chr4.2:1387076-1387095 | MS.gene62677:CDS | 45.0% | |
| GCACAACGTACATAGAAACC+TGG | - | chr4.2:1389003-1389022 | None:intergenic | 45.0% | |
| GTGAGTCTGAGTCAATACAG+AGG | + | chr4.2:1387062-1387081 | MS.gene62677:CDS | 45.0% | |
| TTTCCATGCAACCAGTTTCG+TGG | + | chr4.2:1388463-1388482 | MS.gene62677:CDS | 45.0% | |
| ! | AACCTCGATTTTCAGAGGAG+CGG | - | chr4.2:1388952-1388971 | None:intergenic | 45.0% |
| ! | CATATGTGCGACTTTAGAGG+TGG | - | chr4.2:1387379-1387398 | None:intergenic | 45.0% |
| !! | TTAATATATCAAATTATTAT+AGG | + | chr4.2:1388779-1388798 | MS.gene62677:intron | 5.0% |
| ATGCAACCAGTTTCGTGGAC+AGG | + | chr4.2:1388468-1388487 | MS.gene62677:CDS | 50.0% | |
| CGAAGTATAGCTGGCACCTA+TGG | + | chr4.2:1387510-1387529 | MS.gene62677:intron | 50.0% | |
| GACATTTGGCACACTATGCG+TGG | + | chr4.2:1388606-1388625 | MS.gene62677:intron | 50.0% | |
| TACGCACCCAAACAGTCAAG+AGG | - | chr4.2:1387831-1387850 | None:intergenic | 50.0% | |
| TGTCCACGAAACTGGTTGCA+TGG | - | chr4.2:1388469-1388488 | None:intergenic | 50.0% | |
| TTGACTGTTTGGGTGCGTAG+AGG | + | chr4.2:1387832-1387851 | MS.gene62677:intron | 50.0% | |
| TTTGGGTGCGTAGAGGTTTG+TGG | + | chr4.2:1387839-1387858 | MS.gene62677:intron | 50.0% | |
| TTTGTTCTTGGCCCCTTCGT+GGG | + | chr4.2:1387947-1387966 | MS.gene62677:intron | 50.0% | |
| ! | ATTTTCAGAGGAGCGGTGGT+AGG | - | chr4.2:1388945-1388964 | None:intergenic | 50.0% |
| ! | TTTTGTTCTTGGCCCCTTCG+TGG | + | chr4.2:1387946-1387965 | MS.gene62677:intron | 50.0% |
| !! | TGCGTGGAGATGGTCTTGTT+TGG | + | chr4.2:1388622-1388641 | MS.gene62677:intron | 50.0% |
| CAGTTTCGTGGACAGGAACC+AGG | + | chr4.2:1388475-1388494 | MS.gene62677:CDS | 55.0% | |
| CTGGTTCCTGTCCACGAAAC+TGG | - | chr4.2:1388477-1388496 | None:intergenic | 55.0% | |
| TGGCACACTATGCGTGGAGA+TGG | + | chr4.2:1388612-1388631 | MS.gene62677:intron | 55.0% | |
| ! | CACCGCTCCTCTGAAAATCG+AGG | + | chr4.2:1388947-1388966 | MS.gene62677:CDS | 55.0% |
| ! | CTCGATTTTCAGAGGAGCGG+TGG | - | chr4.2:1388949-1388968 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 1386861 | 1389095 | 1386861 | ID=MS.gene62677 |
| chr4.2 | mRNA | 1386861 | 1389095 | 1386861 | ID=MS.gene62677.t1;Parent=MS.gene62677 |
| chr4.2 | exon | 1386861 | 1386905 | 1386861 | ID=MS.gene62677.t1.exon1;Parent=MS.gene62677.t1 |
| chr4.2 | CDS | 1386861 | 1386905 | 1386861 | ID=cds.MS.gene62677.t1;Parent=MS.gene62677.t1 |
| chr4.2 | exon | 1387044 | 1387120 | 1387044 | ID=MS.gene62677.t1.exon2;Parent=MS.gene62677.t1 |
| chr4.2 | CDS | 1387044 | 1387120 | 1387044 | ID=cds.MS.gene62677.t1;Parent=MS.gene62677.t1 |
| chr4.2 | exon | 1388278 | 1388339 | 1388278 | ID=MS.gene62677.t1.exon3;Parent=MS.gene62677.t1 |
| chr4.2 | CDS | 1388278 | 1388339 | 1388278 | ID=cds.MS.gene62677.t1;Parent=MS.gene62677.t1 |
| chr4.2 | exon | 1388446 | 1388564 | 1388446 | ID=MS.gene62677.t1.exon4;Parent=MS.gene62677.t1 |
| chr4.2 | CDS | 1388446 | 1388564 | 1388446 | ID=cds.MS.gene62677.t1;Parent=MS.gene62677.t1 |
| chr4.2 | exon | 1388801 | 1388968 | 1388801 | ID=MS.gene62677.t1.exon5;Parent=MS.gene62677.t1 |
| chr4.2 | CDS | 1388801 | 1388968 | 1388801 | ID=cds.MS.gene62677.t1;Parent=MS.gene62677.t1 |
| chr4.2 | exon | 1389063 | 1389095 | 1389063 | ID=MS.gene62677.t1.exon6;Parent=MS.gene62677.t1 |
| chr4.2 | CDS | 1389063 | 1389095 | 1389063 | ID=cds.MS.gene62677.t1;Parent=MS.gene62677.t1 |
| Gene Sequence |
| Protein sequence |
