Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82356.t1 | KEH30814.1 | 77.3 | 75 | 6 | 1 | 11 | 74 | 26 | 100 | 4.90E-09 | 70.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82356.t1 | A0A072UM07 | 77.3 | 75 | 6 | 1 | 11 | 74 | 26 | 100 | 3.5e-09 | 70.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene82356 | 0.826525 | 2.59E-54 | -1.69E-46 |
| MS.gene049633 | MS.gene82356 | 0.819216 | 1.30E-52 | -1.69E-46 |
| MS.gene050323 | MS.gene82356 | 0.812816 | 3.50E-51 | -1.69E-46 |
| MS.gene050338 | MS.gene82356 | -0.840786 | 7.05E-58 | -1.69E-46 |
| MS.gene050444 | MS.gene82356 | 0.832176 | 1.10E-55 | -1.69E-46 |
| MS.gene050612 | MS.gene82356 | 0.80953 | 1.81E-50 | -1.69E-46 |
| MS.gene050710 | MS.gene82356 | -0.817945 | 2.53E-52 | -1.69E-46 |
| MS.gene050809 | MS.gene82356 | -0.824128 | 9.55E-54 | -1.69E-46 |
| MS.gene05080 | MS.gene82356 | 0.817932 | 2.55E-52 | -1.69E-46 |
| MS.gene05081 | MS.gene82356 | 0.829515 | 4.93E-55 | -1.69E-46 |
| MS.gene051138 | MS.gene82356 | -0.825643 | 4.19E-54 | -1.69E-46 |
| MS.gene051317 | MS.gene82356 | 0.820745 | 5.83E-53 | -1.69E-46 |
| MS.gene051439 | MS.gene82356 | -0.814243 | 1.70E-51 | -1.69E-46 |
| MS.gene051517 | MS.gene82356 | -0.801266 | 9.76E-49 | -1.69E-46 |
| MS.gene051617 | MS.gene82356 | 0.826524 | 2.59E-54 | -1.69E-46 |
| MS.gene051651 | MS.gene82356 | -0.829873 | 4.03E-55 | -1.69E-46 |
| MS.gene051749 | MS.gene82356 | -0.858985 | 5.66E-63 | -1.69E-46 |
| MS.gene051838 | MS.gene82356 | 0.852371 | 4.82E-61 | -1.69E-46 |
| MS.gene051864 | MS.gene82356 | 0.826779 | 2.25E-54 | -1.69E-46 |
| MS.gene052129 | MS.gene82356 | -0.858294 | 9.09E-63 | -1.69E-46 |
| MS.gene052870 | MS.gene82356 | 0.833888 | 4.12E-56 | -1.69E-46 |
| MS.gene052872 | MS.gene82356 | 0.845298 | 4.43E-59 | -1.69E-46 |
| MS.gene053776 | MS.gene82356 | -0.816558 | 5.20E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene82356 | 0.800959 | 1.13E-48 | -1.69E-46 |
| MS.gene054344 | MS.gene82356 | 0.804138 | 2.49E-49 | -1.69E-46 |
| MS.gene054593 | MS.gene82356 | -0.808839 | 2.54E-50 | -1.69E-46 |
| MS.gene054706 | MS.gene82356 | -0.821925 | 3.12E-53 | -1.69E-46 |
| MS.gene05470 | MS.gene82356 | 0.824851 | 6.45E-54 | -1.69E-46 |
| MS.gene054850 | MS.gene82356 | -0.835835 | 1.33E-56 | -1.69E-46 |
| MS.gene054884 | MS.gene82356 | -0.821713 | 3.49E-53 | -1.69E-46 |
| MS.gene055748 | MS.gene82356 | 0.841897 | 3.60E-58 | -1.69E-46 |
| MS.gene055751 | MS.gene82356 | 0.832959 | 7.02E-56 | -1.69E-46 |
| MS.gene05589 | MS.gene82356 | 0.848963 | 4.38E-60 | -1.69E-46 |
| MS.gene056387 | MS.gene82356 | 0.840441 | 8.68E-58 | -1.69E-46 |
| MS.gene056504 | MS.gene82356 | 0.807194 | 5.70E-50 | -1.69E-46 |
| MS.gene05652 | MS.gene82356 | -0.806086 | 9.76E-50 | -1.69E-46 |
| MS.gene056618 | MS.gene82356 | -0.800916 | 1.15E-48 | -1.69E-46 |
| MS.gene056678 | MS.gene82356 | -0.863453 | 2.46E-64 | -1.69E-46 |
| MS.gene056694 | MS.gene82356 | -0.806418 | 8.31E-50 | -1.69E-46 |
| MS.gene056697 | MS.gene82356 | -0.822267 | 2.60E-53 | -1.69E-46 |
| MS.gene056856 | MS.gene82356 | -0.829607 | 4.68E-55 | -1.69E-46 |
| MS.gene056857 | MS.gene82356 | -0.817903 | 2.59E-52 | -1.69E-46 |
| MS.gene057332 | MS.gene82356 | 0.801133 | 1.04E-48 | -1.69E-46 |
| MS.gene057658 | MS.gene82356 | 0.822963 | 1.79E-53 | -1.69E-46 |
| MS.gene057717 | MS.gene82356 | -0.834839 | 2.38E-56 | -1.69E-46 |
| MS.gene058158 | MS.gene82356 | -0.829762 | 4.29E-55 | -1.69E-46 |
| MS.gene058334 | MS.gene82356 | -0.805755 | 1.15E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene82356 | 0.801424 | 9.06E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene82356 | 0.834247 | 3.35E-56 | -1.69E-46 |
| MS.gene058853 | MS.gene82356 | -0.802065 | 6.70E-49 | -1.69E-46 |
| MS.gene059023 | MS.gene82356 | 0.827453 | 1.55E-54 | -1.69E-46 |
| MS.gene05914 | MS.gene82356 | 0.852719 | 3.84E-61 | -1.69E-46 |
| MS.gene05926 | MS.gene82356 | -0.856707 | 2.68E-62 | -1.69E-46 |
| MS.gene059304 | MS.gene82356 | -0.813291 | 2.76E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene82356 | 0.802623 | 5.14E-49 | -1.69E-46 |
| MS.gene060405 | MS.gene82356 | -0.8333 | 5.77E-56 | -1.69E-46 |
| MS.gene060762 | MS.gene82356 | 0.814898 | 1.22E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene82356 | 0.813478 | 2.51E-51 | -1.69E-46 |
| MS.gene061044 | MS.gene82356 | -0.832415 | 9.58E-56 | -1.69E-46 |
| MS.gene061231 | MS.gene82356 | 0.80538 | 1.37E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82356.t1 | MTR_4g082883 | 77.333 | 75 | 6 | 1 | 11 | 74 | 26 | 100 | 2.38e-32 | 107 |
| MS.gene82356.t1 | MTR_4g082900 | 55.172 | 58 | 26 | 0 | 16 | 73 | 24 | 81 | 8.77e-15 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGGTTTGGCTGGCTCTTC+TGG | 0.238096 | 4.2:+26780912 | None:intergenic |
| TTTGGCTGGCTCTTCTGGTT+TGG | 0.325140 | 4.2:+26780917 | None:intergenic |
| CTGGCTCTTCTGGTTTGGAC+TGG | 0.372877 | 4.2:+26780922 | None:intergenic |
| ACCAATCACACCTTCGGGTT+TGG | 0.373855 | 4.2:+26780899 | None:intergenic |
| AGCACCACCAATCACACCTT+CGG | 0.387425 | 4.2:+26780893 | None:intergenic |
| GCTTCAGCACAAACAACTAT+AGG | 0.435825 | 4.2:-26781008 | MS.gene82356:CDS |
| GCACCACCAATCACACCTTC+GGG | 0.442267 | 4.2:+26780894 | None:intergenic |
| AAAGAAACAATGGCATCAAC+TGG | 0.442857 | 4.2:-26781071 | None:intergenic |
| ATCACACCTTCGGGTTTGGC+TGG | 0.446616 | 4.2:+26780903 | None:intergenic |
| GCCAAACCCGAAGGTGTGAT+TGG | 0.484923 | 4.2:-26780900 | MS.gene82356:CDS |
| CTGGACAAAATCAGCAGCCT+TGG | 0.492880 | 4.2:+26780941 | None:intergenic |
| CTTGGTAGCATACTCTCCTA+CGG | 0.606883 | 4.2:+26780959 | None:intergenic |
| GAAGAGCCAGCCAAACCCGA+AGG | 0.622241 | 4.2:-26780909 | MS.gene82356:CDS |
| CGTAGGAGAGTATGCTACCA+AGG | 0.632082 | 4.2:-26780958 | MS.gene82356:CDS |
| GCTAATGTTCAGAAGACCGT+AGG | 0.649202 | 4.2:-26780975 | MS.gene82356:CDS |
| AAACCCGAAGGTGTGATTGG+TGG | 0.660272 | 4.2:-26780897 | MS.gene82356:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCTGCCAAAAAATCGTTAT+TGG | + | chr4.2:26780879-26780898 | None:intergenic | 35.0% | |
| CCTGCCAAAAAATCGTTATT+GGG | + | chr4.2:26780878-26780897 | None:intergenic | 35.0% | |
| !!! | TGGACCCAATAACGATTTTT+TGG | - | chr4.2:26780871-26780890 | MS.gene82356:CDS | 35.0% |
| GCTTCAGCACAAACAACTAT+AGG | - | chr4.2:26780914-26780933 | MS.gene82356:CDS | 40.0% | |
| !!! | CCCAATAACGATTTTTTGGC+AGG | - | chr4.2:26780875-26780894 | MS.gene82356:CDS | 40.0% |
| GCTAATGTTCAGAAGACCGT+AGG | - | chr4.2:26780947-26780966 | MS.gene82356:CDS | 45.0% | |
| GGTGCTAAAAATGCTGTTGG+AGG | - | chr4.2:26781046-26781065 | MS.gene82356:CDS | 45.0% | |
| ! | CTTGGTAGCATACTCTCCTA+CGG | + | chr4.2:26780966-26780985 | None:intergenic | 45.0% |
| ! | GGTGGTGCTAAAAATGCTGT+TGG | - | chr4.2:26781043-26781062 | MS.gene82356:CDS | 45.0% |
| !!! | CGATTTTTTGGCAGGTGCAA+AGG | - | chr4.2:26780883-26780902 | MS.gene82356:CDS | 45.0% |
| AGCACCACCAATCACACCTT+CGG | + | chr4.2:26781032-26781051 | None:intergenic | 50.0% | |
| CTGGACAAAATCAGCAGCCT+TGG | + | chr4.2:26780984-26781003 | None:intergenic | 50.0% | |
| ! | AAACCCGAAGGTGTGATTGG+TGG | - | chr4.2:26781025-26781044 | MS.gene82356:CDS | 50.0% |
| ! | ACCAATCACACCTTCGGGTT+TGG | + | chr4.2:26781026-26781045 | None:intergenic | 50.0% |
| ! | CGTAGGAGAGTATGCTACCA+AGG | - | chr4.2:26780964-26780983 | MS.gene82356:CDS | 50.0% |
| ! | TTTGGCTGGCTCTTCTGGTT+TGG | + | chr4.2:26781008-26781027 | None:intergenic | 50.0% |
| GCACCACCAATCACACCTTC+GGG | + | chr4.2:26781031-26781050 | None:intergenic | 55.0% | |
| ! | ATCACACCTTCGGGTTTGGC+TGG | + | chr4.2:26781022-26781041 | None:intergenic | 55.0% |
| ! | GCCAAACCCGAAGGTGTGAT+TGG | - | chr4.2:26781022-26781041 | MS.gene82356:CDS | 55.0% |
| !! | CTGGCTCTTCTGGTTTGGAC+TGG | + | chr4.2:26781003-26781022 | None:intergenic | 55.0% |
| GAAGAGCCAGCCAAACCCGA+AGG | - | chr4.2:26781013-26781032 | MS.gene82356:CDS | 60.0% | |
| ! | TCGGGTTTGGCTGGCTCTTC+TGG | + | chr4.2:26781013-26781032 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26780860 | 26781084 | 26780860 | ID=MS.gene82356 |
| chr4.2 | mRNA | 26780860 | 26781084 | 26780860 | ID=MS.gene82356.t1;Parent=MS.gene82356 |
| chr4.2 | exon | 26780860 | 26781084 | 26780860 | ID=MS.gene82356.t1.exon1;Parent=MS.gene82356.t1 |
| chr4.2 | CDS | 26780860 | 26781084 | 26780860 | ID=cds.MS.gene82356.t1;Parent=MS.gene82356.t1 |
| Gene Sequence |
| Protein sequence |
