Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35546.t1 | XP_003595727.2 | 97.8 | 138 | 2 | 1 | 1 | 138 | 1 | 137 | 2.20E-71 | 278.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35546.t1 | Q8RUD6 | 65.7 | 134 | 43 | 3 | 5 | 138 | 36 | 166 | 8.9e-45 | 181.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35546.t1 | G7IGQ0 | 97.8 | 138 | 2 | 1 | 1 | 138 | 1 | 137 | 1.6e-71 | 278.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049147 | MS.gene35546 | 0.81547 | 9.09E-52 | -1.69E-46 |
| MS.gene049171 | MS.gene35546 | -0.806906 | 6.55E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene35546 | -0.836872 | 7.26E-57 | -1.69E-46 |
| MS.gene049222 | MS.gene35546 | -0.814953 | 1.18E-51 | -1.69E-46 |
| MS.gene049259 | MS.gene35546 | 0.806116 | 9.62E-50 | -1.69E-46 |
| MS.gene049265 | MS.gene35546 | 0.816547 | 5.23E-52 | -1.69E-46 |
| MS.gene049267 | MS.gene35546 | 0.811524 | 6.71E-51 | -1.69E-46 |
| MS.gene049285 | MS.gene35546 | -0.810956 | 8.91E-51 | -1.69E-46 |
| MS.gene049320 | MS.gene35546 | -0.828816 | 7.29E-55 | -1.69E-46 |
| MS.gene049402 | MS.gene35546 | 0.821982 | 3.02E-53 | -1.69E-46 |
| MS.gene049549 | MS.gene35546 | 0.802143 | 6.45E-49 | -1.69E-46 |
| MS.gene049611 | MS.gene35546 | 0.857975 | 1.13E-62 | -1.69E-46 |
| MS.gene049633 | MS.gene35546 | -0.805344 | 1.40E-49 | -1.69E-46 |
| MS.gene049634 | MS.gene35546 | 0.865186 | 7.09E-65 | -1.69E-46 |
| MS.gene050082 | MS.gene35546 | 0.802083 | 6.64E-49 | -1.69E-46 |
| MS.gene050096 | MS.gene35546 | 0.809289 | 2.04E-50 | -1.69E-46 |
| MS.gene050163 | MS.gene35546 | -0.826741 | 2.30E-54 | -1.69E-46 |
| MS.gene050165 | MS.gene35546 | -0.838131 | 3.45E-57 | -1.69E-46 |
| MS.gene050173 | MS.gene35546 | 0.822506 | 2.28E-53 | -1.69E-46 |
| MS.gene050216 | MS.gene35546 | -0.801334 | 9.46E-49 | -1.69E-46 |
| MS.gene050323 | MS.gene35546 | -0.809687 | 1.67E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene35546 | -0.831478 | 1.63E-55 | -1.69E-46 |
| MS.gene050381 | MS.gene35546 | 0.822161 | 2.75E-53 | -1.69E-46 |
| MS.gene050444 | MS.gene35546 | -0.868705 | 5.35E-66 | -1.69E-46 |
| MS.gene050504 | MS.gene35546 | -0.830874 | 2.30E-55 | -1.69E-46 |
| MS.gene050612 | MS.gene35546 | -0.848307 | 6.66E-60 | -1.69E-46 |
| MS.gene050675 | MS.gene35546 | -0.823105 | 1.66E-53 | -1.69E-46 |
| MS.gene050772 | MS.gene35546 | -0.80159 | 8.38E-49 | -1.69E-46 |
| MS.gene05079 | MS.gene35546 | 0.888705 | 4.66E-73 | -1.69E-46 |
| MS.gene050809 | MS.gene35546 | 0.859019 | 5.52E-63 | -1.69E-46 |
| MS.gene05080 | MS.gene35546 | -0.807297 | 5.41E-50 | -1.69E-46 |
| MS.gene050832 | MS.gene35546 | 0.801326 | 9.49E-49 | -1.69E-46 |
| MS.gene050833 | MS.gene35546 | 0.820206 | 7.75E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene35546 | -0.826759 | 2.27E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene35546 | -0.82778 | 1.29E-54 | -1.69E-46 |
| MS.gene050986 | MS.gene35546 | -0.801759 | 7.74E-49 | -1.69E-46 |
| MS.gene051044 | MS.gene35546 | 0.801216 | 1.00E-48 | -1.69E-46 |
| MS.gene05104 | MS.gene35546 | 0.857775 | 1.30E-62 | -1.69E-46 |
| MS.gene051127 | MS.gene35546 | 0.822531 | 2.25E-53 | -1.69E-46 |
| MS.gene051138 | MS.gene35546 | 0.880098 | 7.26E-70 | -1.69E-46 |
| MS.gene05114 | MS.gene35546 | 0.821287 | 4.37E-53 | -1.69E-46 |
| MS.gene051228 | MS.gene35546 | -0.844098 | 9.33E-59 | -1.69E-46 |
| MS.gene051268 | MS.gene35546 | -0.815406 | 9.40E-52 | -1.69E-46 |
| MS.gene051422 | MS.gene35546 | 0.804589 | 2.01E-49 | -1.69E-46 |
| MS.gene051617 | MS.gene35546 | -0.804347 | 2.26E-49 | -1.69E-46 |
| MS.gene051632 | MS.gene35546 | 0.815046 | 1.13E-51 | -1.69E-46 |
| MS.gene051651 | MS.gene35546 | 0.827402 | 1.60E-54 | -1.69E-46 |
| MS.gene051810 | MS.gene35546 | -0.838244 | 3.23E-57 | -1.69E-46 |
| MS.gene051858 | MS.gene35546 | -0.815925 | 7.20E-52 | -1.69E-46 |
| MS.gene051863 | MS.gene35546 | 0.860841 | 1.56E-63 | -1.69E-46 |
| MS.gene051864 | MS.gene35546 | -0.80024 | 1.58E-48 | -1.69E-46 |
| MS.gene051874 | MS.gene35546 | -0.803638 | 3.17E-49 | -1.69E-46 |
| MS.gene051949 | MS.gene35546 | -0.818224 | 2.19E-52 | -1.69E-46 |
| MS.gene052129 | MS.gene35546 | 0.803068 | 4.16E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene35546 | -0.801344 | 9.41E-49 | -1.69E-46 |
| MS.gene052379 | MS.gene35546 | -0.829016 | 6.52E-55 | -1.69E-46 |
| MS.gene05240 | MS.gene35546 | 0.800512 | 1.39E-48 | -1.69E-46 |
| MS.gene05244 | MS.gene35546 | 0.8125 | 4.11E-51 | -1.69E-46 |
| MS.gene052805 | MS.gene35546 | 0.833918 | 4.05E-56 | -1.69E-46 |
| MS.gene052870 | MS.gene35546 | -0.826209 | 3.08E-54 | -1.69E-46 |
| MS.gene052872 | MS.gene35546 | -0.839533 | 1.50E-57 | -1.69E-46 |
| MS.gene053420 | MS.gene35546 | 0.853764 | 1.93E-61 | -1.69E-46 |
| MS.gene053488 | MS.gene35546 | 0.839797 | 1.28E-57 | -1.69E-46 |
| MS.gene053561 | MS.gene35546 | 0.850812 | 1.33E-60 | -1.69E-46 |
| MS.gene053632 | MS.gene35546 | -0.819421 | 1.17E-52 | -1.69E-46 |
| MS.gene053776 | MS.gene35546 | 0.839644 | 1.40E-57 | -1.69E-46 |
| MS.gene053824 | MS.gene35546 | -0.80084 | 1.19E-48 | -1.69E-46 |
| MS.gene053916 | MS.gene35546 | -0.827883 | 1.22E-54 | -1.69E-46 |
| MS.gene054290 | MS.gene35546 | 0.819871 | 9.25E-53 | -1.69E-46 |
| MS.gene054616 | MS.gene35546 | 0.812214 | 4.74E-51 | -1.69E-46 |
| MS.gene054642 | MS.gene35546 | -0.817474 | 3.24E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene35546 | 0.833276 | 5.85E-56 | -1.69E-46 |
| MS.gene054707 | MS.gene35546 | 0.833059 | 6.63E-56 | -1.69E-46 |
| MS.gene054708 | MS.gene35546 | 0.818333 | 2.07E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene35546 | -0.815736 | 7.93E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene35546 | -0.801566 | 8.48E-49 | -1.69E-46 |
| MS.gene055111 | MS.gene35546 | 0.843985 | 1.00E-58 | -1.69E-46 |
| MS.gene055124 | MS.gene35546 | 0.806783 | 6.96E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene35546 | 0.836697 | 8.04E-57 | -1.69E-46 |
| MS.gene055202 | MS.gene35546 | 0.820337 | 7.23E-53 | -1.69E-46 |
| MS.gene055206 | MS.gene35546 | -0.825612 | 4.26E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene35546 | -0.81486 | 1.24E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene35546 | -0.823656 | 1.23E-53 | -1.69E-46 |
| MS.gene055577 | MS.gene35546 | -0.824469 | 7.94E-54 | -1.69E-46 |
| MS.gene055748 | MS.gene35546 | -0.81513 | 1.08E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene35546 | -0.820683 | 6.03E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene35546 | -0.812771 | 3.59E-51 | -1.69E-46 |
| MS.gene055956 | MS.gene35546 | 0.808222 | 3.44E-50 | -1.69E-46 |
| MS.gene055959 | MS.gene35546 | 0.806151 | 9.46E-50 | -1.69E-46 |
| MS.gene056224 | MS.gene35546 | 0.842372 | 2.69E-58 | -1.69E-46 |
| MS.gene056387 | MS.gene35546 | -0.821389 | 4.14E-53 | -1.69E-46 |
| MS.gene056487 | MS.gene35546 | -0.81599 | 6.96E-52 | -1.69E-46 |
| MS.gene056504 | MS.gene35546 | -0.816263 | 6.05E-52 | -1.69E-46 |
| MS.gene056590 | MS.gene35546 | 0.808963 | 2.39E-50 | -1.69E-46 |
| MS.gene056591 | MS.gene35546 | 0.808883 | 2.49E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene35546 | -0.82243 | 2.38E-53 | -1.69E-46 |
| MS.gene056856 | MS.gene35546 | 0.869613 | 2.72E-66 | -1.69E-46 |
| MS.gene056857 | MS.gene35546 | 0.843017 | 1.81E-58 | -1.69E-46 |
| MS.gene056903 | MS.gene35546 | -0.812072 | 5.10E-51 | -1.69E-46 |
| MS.gene057246 | MS.gene35546 | -0.82564 | 4.20E-54 | -1.69E-46 |
| MS.gene057717 | MS.gene35546 | 0.821281 | 4.39E-53 | -1.69E-46 |
| MS.gene057857 | MS.gene35546 | -0.806789 | 6.94E-50 | -1.69E-46 |
| MS.gene057926 | MS.gene35546 | -0.821434 | 4.05E-53 | -1.69E-46 |
| MS.gene058067 | MS.gene35546 | 0.811857 | 5.67E-51 | -1.69E-46 |
| MS.gene058068 | MS.gene35546 | 0.823486 | 1.35E-53 | -1.69E-46 |
| MS.gene058093 | MS.gene35546 | 0.838045 | 3.63E-57 | -1.69E-46 |
| MS.gene058099 | MS.gene35546 | 0.860857 | 1.54E-63 | -1.69E-46 |
| MS.gene058117 | MS.gene35546 | 0.811407 | 7.11E-51 | -1.69E-46 |
| MS.gene058157 | MS.gene35546 | -0.801851 | 7.41E-49 | -1.69E-46 |
| MS.gene058158 | MS.gene35546 | 0.802743 | 4.85E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene35546 | 0.815131 | 1.08E-51 | -1.69E-46 |
| MS.gene058173 | MS.gene35546 | 0.800666 | 1.30E-48 | -1.69E-46 |
| MS.gene058175 | MS.gene35546 | 0.822143 | 2.77E-53 | -1.69E-46 |
| MS.gene058268 | MS.gene35546 | 0.805504 | 1.29E-49 | -1.69E-46 |
| MS.gene058269 | MS.gene35546 | 0.809359 | 1.97E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene35546 | -0.801797 | 7.60E-49 | -1.69E-46 |
| MS.gene058416 | MS.gene35546 | 0.815481 | 9.04E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene35546 | -0.810117 | 1.35E-50 | -1.69E-46 |
| MS.gene058452 | MS.gene35546 | 0.806481 | 8.06E-50 | -1.69E-46 |
| MS.gene058588 | MS.gene35546 | -0.809292 | 2.03E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene35546 | 0.835495 | 1.62E-56 | -1.69E-46 |
| MS.gene058723 | MS.gene35546 | -0.834822 | 2.40E-56 | -1.69E-46 |
| MS.gene058773 | MS.gene35546 | -0.835819 | 1.34E-56 | -1.69E-46 |
| MS.gene058838 | MS.gene35546 | -0.802901 | 4.50E-49 | -1.69E-46 |
| MS.gene058840 | MS.gene35546 | 0.860921 | 1.47E-63 | -1.69E-46 |
| MS.gene059022 | MS.gene35546 | -0.835035 | 2.12E-56 | -1.69E-46 |
| MS.gene059102 | MS.gene35546 | 0.811987 | 5.32E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene35546 | -0.833229 | 6.01E-56 | -1.69E-46 |
| MS.gene059677 | MS.gene35546 | -0.836407 | 9.53E-57 | -1.69E-46 |
| MS.gene059682 | MS.gene35546 | 0.811146 | 8.10E-51 | -1.69E-46 |
| MS.gene059699 | MS.gene35546 | 0.807611 | 4.64E-50 | -1.69E-46 |
| MS.gene059703 | MS.gene35546 | 0.804712 | 1.89E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene35546 | -0.812904 | 3.35E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene35546 | -0.826349 | 2.85E-54 | -1.69E-46 |
| MS.gene059902 | MS.gene35546 | -0.808312 | 3.29E-50 | -1.69E-46 |
| MS.gene059943 | MS.gene35546 | 0.800619 | 1.32E-48 | -1.69E-46 |
| MS.gene060341 | MS.gene35546 | -0.801849 | 7.42E-49 | -1.69E-46 |
| MS.gene060405 | MS.gene35546 | 0.840995 | 6.21E-58 | -1.69E-46 |
| MS.gene06074 | MS.gene35546 | 0.845463 | 4.00E-59 | -1.69E-46 |
| MS.gene060762 | MS.gene35546 | -0.845194 | 4.73E-59 | -1.69E-46 |
| MS.gene060818 | MS.gene35546 | -0.816124 | 6.50E-52 | -1.69E-46 |
| MS.gene060823 | MS.gene35546 | 0.805338 | 1.40E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene35546 | -0.804285 | 2.32E-49 | -1.69E-46 |
| MS.gene061134 | MS.gene35546 | -0.800768 | 1.23E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35546.t1 | MTR_2g059890 | 97.826 | 138 | 2 | 1 | 1 | 138 | 1 | 137 | 2.20e-98 | 278 |
| MS.gene35546.t1 | MTR_2g017630 | 89.130 | 138 | 14 | 1 | 1 | 138 | 1 | 137 | 2.76e-89 | 255 |
| MS.gene35546.t1 | MTR_2g059890 | 97.980 | 99 | 2 | 0 | 1 | 99 | 1 | 99 | 1.54e-68 | 202 |
| MS.gene35546.t1 | MTR_2g059890 | 97.000 | 100 | 3 | 0 | 1 | 100 | 1 | 100 | 2.62e-68 | 202 |
| MS.gene35546.t1 | MTR_2g059890 | 97.000 | 100 | 3 | 0 | 1 | 100 | 1 | 100 | 4.01e-68 | 203 |
| MS.gene35546.t1 | MTR_8g075420 | 54.386 | 114 | 48 | 3 | 9 | 120 | 12 | 123 | 8.70e-36 | 120 |
| MS.gene35546.t1 | MTR_5g022390 | 54.128 | 109 | 46 | 3 | 10 | 116 | 30 | 136 | 1.14e-31 | 110 |
| MS.gene35546.t1 | MTR_8g075420 | 55.556 | 90 | 37 | 2 | 9 | 96 | 12 | 100 | 9.86e-28 | 99.0 |
| MS.gene35546.t1 | MTR_5g023170 | 41.284 | 109 | 62 | 2 | 12 | 120 | 75 | 181 | 9.15e-25 | 94.0 |
| MS.gene35546.t1 | MTR_5g022390 | 56.000 | 75 | 30 | 2 | 10 | 82 | 30 | 103 | 1.76e-19 | 78.6 |
| MS.gene35546.t1 | MTR_5g023170 | 43.529 | 85 | 47 | 1 | 12 | 96 | 75 | 158 | 7.55e-19 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35546.t1 | AT2G21045 | 64.286 | 140 | 45 | 4 | 1 | 138 | 30 | 166 | 1.81e-59 | 181 |
| MS.gene35546.t1 | AT5G66170 | 42.623 | 122 | 66 | 3 | 1 | 120 | 5 | 124 | 6.48e-31 | 108 |
| MS.gene35546.t1 | AT5G66170 | 42.276 | 123 | 67 | 3 | 1 | 120 | 5 | 126 | 9.40e-31 | 107 |
| MS.gene35546.t1 | AT2G17850 | 47.500 | 120 | 59 | 3 | 3 | 120 | 25 | 142 | 5.06e-30 | 106 |
| MS.gene35546.t1 | AT5G66040 | 44.954 | 109 | 58 | 2 | 12 | 120 | 10 | 116 | 4.64e-26 | 95.1 |
| MS.gene35546.t1 | AT4G35770 | 41.667 | 108 | 57 | 2 | 22 | 129 | 81 | 182 | 5.32e-24 | 91.7 |
| MS.gene35546.t1 | AT2G17850 | 37.500 | 152 | 59 | 4 | 3 | 120 | 25 | 174 | 3.07e-23 | 90.1 |
| MS.gene35546.t1 | AT5G66170 | 40.816 | 98 | 55 | 2 | 1 | 96 | 5 | 101 | 1.35e-22 | 87.0 |
| MS.gene35546.t1 | AT2G17850 | 47.826 | 92 | 45 | 2 | 3 | 92 | 25 | 115 | 2.41e-22 | 86.3 |
| MS.gene35546.t1 | AT4G35770 | 45.333 | 75 | 41 | 0 | 22 | 96 | 81 | 155 | 5.81e-18 | 75.5 |
| MS.gene35546.t1 | AT4G35770 | 45.333 | 75 | 41 | 0 | 22 | 96 | 81 | 155 | 1.10e-17 | 75.5 |
| MS.gene35546.t1 | AT4G35770 | 46.667 | 60 | 32 | 0 | 22 | 81 | 81 | 140 | 2.40e-14 | 66.2 |
| MS.gene35546.t1 | AT5G66040 | 45.000 | 60 | 32 | 1 | 61 | 120 | 3 | 61 | 9.24e-12 | 57.0 |
Find 32 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTACTTGGTTAACAAAATC+TGG | 0.229853 | 4.2:-63035150 | None:intergenic |
| GTCATAGGCTTACTGAATTA+TGG | 0.231271 | 4.2:-63035365 | None:intergenic |
| TTCTTCTGTGCTGAACAAAT+AGG | 0.299247 | 4.2:-63034853 | None:intergenic |
| TCTTCTGTGCTGAACAAATA+GGG | 0.319017 | 4.2:-63034852 | None:intergenic |
| TTGAAACTCTTACTCCTTTG+TGG | 0.372195 | 4.2:-63034518 | None:intergenic |
| CAAATTCAGGCTTGTAATGC+TGG | 0.378391 | 4.2:+63035312 | MS.gene35546:intron |
| TGGGTGGATGCTGGATTTGC+AGG | 0.391447 | 4.2:+63036750 | MS.gene35546:CDS |
| AGATGAATCTAGCAGATCTT+TGG | 0.407928 | 4.2:-63034657 | None:intergenic |
| GGGAGGCTACTCTGCGTGGG+TGG | 0.422325 | 4.2:+63036734 | MS.gene35546:CDS |
| TTTGGTAGCATGGACATCTA+AGG | 0.431769 | 4.2:-63034639 | None:intergenic |
| TCTTATATATTGCTTCTACT+TGG | 0.436133 | 4.2:-63035164 | None:intergenic |
| GAGGAAGATCAAGTCGAGCC+TGG | 0.438094 | 4.2:+63035334 | MS.gene35546:CDS |
| TACTCTGCGTGGGTGGATGC+TGG | 0.443842 | 4.2:+63036741 | MS.gene35546:CDS |
| AAAGATCTGCTAGATTCATC+TGG | 0.488656 | 4.2:+63034659 | MS.gene35546:CDS |
| TATTTGTTCAGCACAGAAGA+AGG | 0.505003 | 4.2:+63034855 | MS.gene35546:CDS |
| CGTTAATAGCATTCTCAACA+TGG | 0.507094 | 4.2:-63034827 | None:intergenic |
| ATTTAAACATATTGTTAACA+TGG | 0.515720 | 4.2:+63036710 | MS.gene35546:CDS |
| CTAGCAGATCTTTGGTAGCA+TGG | 0.528750 | 4.2:-63034649 | None:intergenic |
| TTTAAACATATTGTTAACAT+GGG | 0.557076 | 4.2:+63036711 | MS.gene35546:CDS |
| AGGAAGATCAAGTCGAGCCT+GGG | 0.580327 | 4.2:+63035335 | MS.gene35546:CDS |
| TGAATTATGGAGATCAACCC+AGG | 0.586135 | 4.2:-63035352 | None:intergenic |
| AAACATATTGTTAACATGGG+GGG | 0.595696 | 4.2:+63036714 | MS.gene35546:CDS |
| AGAAGCAATATATAAGAGTG+AGG | 0.598950 | 4.2:+63035170 | MS.gene35546:CDS |
| CTTCTGTGCTGAACAAATAG+GGG | 0.608024 | 4.2:-63034851 | None:intergenic |
| GAGTGAGGATCACTTGATTG+TGG | 0.613966 | 4.2:+63035185 | MS.gene35546:CDS |
| GTTACAATTATCTTGATGTG+AGG | 0.616591 | 4.2:+63034681 | MS.gene35546:CDS |
| TTAAACATATTGTTAACATG+GGG | 0.636631 | 4.2:+63036712 | MS.gene35546:CDS |
| TAAACATATTGTTAACATGG+GGG | 0.658767 | 4.2:+63036713 | MS.gene35546:CDS |
| AAATATAATGGACACCACAA+AGG | 0.664806 | 4.2:+63034504 | None:intergenic |
| ATTCAGGCTTGTAATGCTGG+AGG | 0.667532 | 4.2:+63035315 | MS.gene35546:intron |
| CCACTGAAATAGGACTGTGG+AGG | 0.675906 | 4.2:+63034791 | MS.gene35546:intron |
| TTTCCACTGAAATAGGACTG+TGG | 0.676448 | 4.2:+63034788 | MS.gene35546:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAAAAAAATGAATCTAA+TGG | - | chr4.2:63035484-63035503 | None:intergenic | 10.0% |
| !! | CAAAAATAAATAAAAATAGT+AGG | + | chr4.2:63036158-63036177 | MS.gene35546:intron | 10.0% |
| !! | CTAAAATAAAAAAAACTATT+AGG | + | chr4.2:63035611-63035630 | MS.gene35546:intron | 10.0% |
| !! | TATTAATTTACCAAAAAATT+AGG | + | chr4.2:63035428-63035447 | MS.gene35546:intron | 10.0% |
| !! | TTAAAAAAAATGAATCTAAT+GGG | - | chr4.2:63035483-63035502 | None:intergenic | 10.0% |
| !!! | TTGAAAAAAAAATAGATTTT+GGG | - | chr4.2:63035643-63035662 | None:intergenic | 10.0% |
| !!! | TTTGAAAAAAAAATAGATTT+TGG | - | chr4.2:63035644-63035663 | None:intergenic | 10.0% |
| !!! | TTTTTTTTTCAAATGATTAA+GGG | - | chr4.2:63036042-63036061 | None:intergenic | 10.0% |
| !!! | TTTTTTTTTTCAAATGATTA+AGG | - | chr4.2:63036043-63036062 | None:intergenic | 10.0% |
| !! | AAAATTAAAAATGAAGAAAC+TGG | - | chr4.2:63034722-63034741 | None:intergenic | 15.0% |
| !! | AAATTAAAAATGAAGAAACT+GGG | - | chr4.2:63034721-63034740 | None:intergenic | 15.0% |
| !! | AAGTAATTAATTATTACTGA+TGG | - | chr4.2:63036203-63036222 | None:intergenic | 15.0% |
| !! | AGTAATTAATTATTACTGAT+GGG | - | chr4.2:63036202-63036221 | None:intergenic | 15.0% |
| !! | ATTTAAACATATTGTTAACA+TGG | + | chr4.2:63036710-63036729 | MS.gene35546:CDS | 15.0% |
| !! | TAAAAAAAATGAATCTAATG+GGG | - | chr4.2:63035482-63035501 | None:intergenic | 15.0% |
| !! | TTTAAACATATTGTTAACAT+GGG | + | chr4.2:63036711-63036730 | MS.gene35546:CDS | 15.0% |
| !!! | AAATAATTTTGAATGCTTAA+TGG | - | chr4.2:63036649-63036668 | None:intergenic | 15.0% |
| !!! | TAATTGAAATCCTAATTTTT+TGG | - | chr4.2:63035441-63035460 | None:intergenic | 15.0% |
| !! | AAAAAGAAAGATAAAGACTT+TGG | - | chr4.2:63034770-63034789 | None:intergenic | 20.0% |
| !! | AACAAAACAAAAAAACAAAC+AGG | + | chr4.2:63036016-63036035 | MS.gene35546:intron | 20.0% |
| !! | ATCCATAACATAATATAGAA+TGG | - | chr4.2:63034556-63034575 | None:intergenic | 20.0% |
| !! | ATGAATAATTCTATTTAGTG+AGG | + | chr4.2:63036127-63036146 | MS.gene35546:intron | 20.0% |
| !! | TAACATAATATAGAATGGAA+AGG | - | chr4.2:63034551-63034570 | None:intergenic | 20.0% |
| !! | TAATCAAAATTGAGAATTTG+AGG | - | chr4.2:63035394-63035413 | None:intergenic | 20.0% |
| !! | TATTGTTATGTTTATTGCTA+AGG | + | chr4.2:63036076-63036095 | MS.gene35546:intron | 20.0% |
| !! | TGAATAATTCTATTTAGTGA+GGG | + | chr4.2:63036128-63036147 | MS.gene35546:intron | 20.0% |
| !! | TGTATACATAAATATCTAAC+TGG | + | chr4.2:63035052-63035071 | MS.gene35546:intron | 20.0% |
| !! | TTAAACATATTGTTAACATG+GGG | + | chr4.2:63036712-63036731 | MS.gene35546:CDS | 20.0% |
| !! | TTCCATTCTATATTATGTTA+TGG | + | chr4.2:63034551-63034570 | MS.gene35546:intron | 20.0% |
| !!! | ATTCATTTATTTTGAGCAAA+TGG | - | chr4.2:63036113-63036132 | None:intergenic | 20.0% |
| !!! | TTATAATGGATTACAAATTC+AGG | + | chr4.2:63035299-63035318 | MS.gene35546:intron | 20.0% |
| !!! | TTTTTTTTTCAAATACATGC+AGG | + | chr4.2:63035651-63035670 | MS.gene35546:intron | 20.0% |
| ! | ACACTGATGTATGATTATAA+TGG | + | chr4.2:63035285-63035304 | MS.gene35546:intron | 25.0% |
| ! | GAATTGGAGATGATAATAAA+TGG | + | chr4.2:63036266-63036285 | MS.gene35546:intron | 25.0% |
| ! | GTTATTGACAAATGATTAGT+CGG | - | chr4.2:63036537-63036556 | None:intergenic | 25.0% |
| ! | TAAAACAATATGCACAAATC+AGG | + | chr4.2:63035244-63035263 | MS.gene35546:intron | 25.0% |
| ! | TAAACATATTGTTAACATGG+GGG | + | chr4.2:63036713-63036732 | MS.gene35546:CDS | 25.0% |
| ! | TCTTATATATTGCTTCTACT+TGG | - | chr4.2:63035167-63035186 | None:intergenic | 25.0% |
| ! | TTACAAAGCTAAATCAAAAC+AGG | - | chr4.2:63035992-63036011 | None:intergenic | 25.0% |
| !! | GATTTTGTGTTAGAAAAATC+TGG | - | chr4.2:63035773-63035792 | None:intergenic | 25.0% |
| !!! | TTTCTTTTTTCCACTGAAAT+AGG | + | chr4.2:63034781-63034800 | MS.gene35546:intron | 25.0% |
| AAACATATTGTTAACATGGG+GGG | + | chr4.2:63036714-63036733 | MS.gene35546:CDS | 30.0% | |
| AAGAAATTTGTGCATGCTAA+AGG | - | chr4.2:63036506-63036525 | None:intergenic | 30.0% | |
| AGAAATTTGTGCATGCTAAA+GGG | - | chr4.2:63036505-63036524 | None:intergenic | 30.0% | |
| AGAAGCAATATATAAGAGTG+AGG | + | chr4.2:63035170-63035189 | MS.gene35546:CDS | 30.0% | |
| ATCTCTCCAAATGAAAGAAT+TGG | + | chr4.2:63036250-63036269 | MS.gene35546:intron | 30.0% | |
| GTTACAATTATCTTGATGTG+AGG | + | chr4.2:63034681-63034700 | MS.gene35546:CDS | 30.0% | |
| TCATCTCCAATTCTTTCATT+TGG | - | chr4.2:63036259-63036278 | None:intergenic | 30.0% | |
| TCTACTTGGTTAACAAAATC+TGG | - | chr4.2:63035153-63035172 | None:intergenic | 30.0% | |
| TGATGTGAGGTAATTAACAA+AGG | + | chr4.2:63034694-63034713 | MS.gene35546:intron | 30.0% | |
| TTGTGTATGTTGAAGTTACA+TGG | - | chr4.2:63036329-63036348 | None:intergenic | 30.0% | |
| ! | AATCCATTTTGTGTACGTAA+TGG | + | chr4.2:63036406-63036425 | MS.gene35546:intron | 30.0% |
| ! | ATCCATTTTGTGTACGTAAT+GGG | + | chr4.2:63036407-63036426 | MS.gene35546:intron | 30.0% |
| ! | ATTCAATTTTCTGCTCCTTA+TGG | + | chr4.2:63034910-63034929 | MS.gene35546:intron | 30.0% |
| ! | CAAAATTGAGAATTTGAGGT+TGG | - | chr4.2:63035390-63035409 | None:intergenic | 30.0% |
| ! | TTCAATTTTCTGCTCCTTAT+GGG | + | chr4.2:63034911-63034930 | MS.gene35546:intron | 30.0% |
| ! | TTTGAATGCTTAATGGATCA+TGG | - | chr4.2:63036642-63036661 | None:intergenic | 30.0% |
| AAAGATCTGCTAGATTCATC+TGG | + | chr4.2:63034659-63034678 | MS.gene35546:CDS | 35.0% | |
| AGATGAATCTAGCAGATCTT+TGG | - | chr4.2:63034660-63034679 | None:intergenic | 35.0% | |
| ATAGAAAAGCTCAAGCTCTT+AGG | - | chr4.2:63035944-63035963 | None:intergenic | 35.0% | |
| ATGATCTCATAGACATGCTT+AGG | + | chr4.2:63035895-63035914 | MS.gene35546:intron | 35.0% | |
| ATGCTTAGGAATGATGCTAT+GGG | + | chr4.2:63035909-63035928 | MS.gene35546:intron | 35.0% | |
| CAATATGTTTAAATCCCTGC+TGG | - | chr4.2:63036704-63036723 | None:intergenic | 35.0% | |
| CGTTAATAGCATTCTCAACA+TGG | - | chr4.2:63034830-63034849 | None:intergenic | 35.0% | |
| CTTACAAATGATCAACAGGA+CGG | + | chr4.2:63035118-63035137 | MS.gene35546:intron | 35.0% | |
| GATGTTGTAATTAACCAGCA+GGG | + | chr4.2:63036687-63036706 | MS.gene35546:intron | 35.0% | |
| GTCATAGGCTTACTGAATTA+TGG | - | chr4.2:63035368-63035387 | None:intergenic | 35.0% | |
| TAGCTAGCGATAAATGAGTT+AGG | - | chr4.2:63036294-63036313 | None:intergenic | 35.0% | |
| TATTTGTTCAGCACAGAAGA+AGG | + | chr4.2:63034855-63034874 | MS.gene35546:CDS | 35.0% | |
| TCTTCTGTGCTGAACAAATA+GGG | - | chr4.2:63034855-63034874 | None:intergenic | 35.0% | |
| TGATGTTGTAATTAACCAGC+AGG | + | chr4.2:63036686-63036705 | MS.gene35546:intron | 35.0% | |
| TGTGCTTACAAATGATCAAC+AGG | + | chr4.2:63035114-63035133 | MS.gene35546:intron | 35.0% | |
| TTACAAATGATCAACAGGAC+GGG | + | chr4.2:63035119-63035138 | MS.gene35546:intron | 35.0% | |
| TTCTTCTGTGCTGAACAAAT+AGG | - | chr4.2:63034856-63034875 | None:intergenic | 35.0% | |
| TTGAAACTCTTACTCCTTTG+TGG | - | chr4.2:63034521-63034540 | None:intergenic | 35.0% | |
| ! | AATGCTTAATGGATCATGGA+GGG | - | chr4.2:63036638-63036657 | None:intergenic | 35.0% |
| ! | CAAAATGGATTTCTCTATCG+TGG | - | chr4.2:63036397-63036416 | None:intergenic | 35.0% |
| ! | GACAAATGATTAGTCGGTTT+CGG | - | chr4.2:63036531-63036550 | None:intergenic | 35.0% |
| ! | TCTATCGTGGATGCTTTTAA+TGG | - | chr4.2:63036384-63036403 | None:intergenic | 35.0% |
| !! | TTCGTCTAATGTTGGTTTCT+TGG | + | chr4.2:63035854-63035873 | MS.gene35546:intron | 35.0% |
| AGCATGTCTATGAGATCATG+AGG | - | chr4.2:63035894-63035913 | None:intergenic | 40.0% | |
| ATGTCTATGAGATCATGAGG+AGG | - | chr4.2:63035891-63035910 | None:intergenic | 40.0% | |
| CAAATTCAGGCTTGTAATGC+TGG | + | chr4.2:63035312-63035331 | MS.gene35546:intron | 40.0% | |
| CAGCACAGAAGAAGGTAATA+TGG | + | chr4.2:63034863-63034882 | MS.gene35546:intron | 40.0% | |
| CATGCTTAGGAATGATGCTA+TGG | + | chr4.2:63035908-63035927 | MS.gene35546:intron | 40.0% | |
| CTCATTTATCGCTAGCTAAG+TGG | + | chr4.2:63036296-63036315 | MS.gene35546:intron | 40.0% | |
| CTTCTGTGCTGAACAAATAG+GGG | - | chr4.2:63034854-63034873 | None:intergenic | 40.0% | |
| GACCCATTACGTACACAAAA+TGG | - | chr4.2:63036412-63036431 | None:intergenic | 40.0% | |
| TGAATTATGGAGATCAACCC+AGG | - | chr4.2:63035355-63035374 | None:intergenic | 40.0% | |
| TGTCTATGAGATCATGAGGA+GGG | - | chr4.2:63035890-63035909 | None:intergenic | 40.0% | |
| TTTCCACTGAAATAGGACTG+TGG | + | chr4.2:63034788-63034807 | MS.gene35546:intron | 40.0% | |
| ! | GAATGCTTAATGGATCATGG+AGG | - | chr4.2:63036639-63036658 | None:intergenic | 40.0% |
| ! | GAGAATTTGAGGTTGGTCAT+AGG | - | chr4.2:63035383-63035402 | None:intergenic | 40.0% |
| ! | TTAAAGAGGTGTAAGACTCG+CGG | + | chr4.2:63036589-63036608 | MS.gene35546:intron | 40.0% |
| ! | TTTGGTAGCATGGACATCTA+AGG | - | chr4.2:63034642-63034661 | None:intergenic | 40.0% |
| !!! | CTTGTTTTAAGCTCTTCTGC+AGG | - | chr4.2:63036786-63036805 | None:intergenic | 40.0% |
| AGCTTGGCCAAGAAATCATG+AGG | - | chr4.2:63035828-63035847 | None:intergenic | 45.0% | |
| ATTAGACGAAGCTCCTAGCT+TGG | - | chr4.2:63035844-63035863 | None:intergenic | 45.0% | |
| ATTCAGGCTTGTAATGCTGG+AGG | + | chr4.2:63035315-63035334 | MS.gene35546:intron | 45.0% | |
| CATATTGTTAACATGGGGGG+AGG | + | chr4.2:63036717-63036736 | MS.gene35546:CDS | 45.0% | |
| CATGATTTCTTGGCCAAGCT+AGG | + | chr4.2:63035828-63035847 | MS.gene35546:intron | 45.0% | |
| CTAGGAGCTTCGTCTAATGT+TGG | + | chr4.2:63035846-63035865 | MS.gene35546:intron | 45.0% | |
| GACCCTTCCTCATGATTTCT+TGG | + | chr4.2:63035818-63035837 | MS.gene35546:intron | 45.0% | |
| GAGTGAGGATCACTTGATTG+TGG | + | chr4.2:63035185-63035204 | MS.gene35546:CDS | 45.0% | |
| GGCCAAGAAATCATGAGGAA+GGG | - | chr4.2:63035823-63035842 | None:intergenic | 45.0% | |
| GTCTATGAGATCATGAGGAG+GGG | - | chr4.2:63035889-63035908 | None:intergenic | 45.0% | |
| TGGCCAAGAAATCATGAGGA+AGG | - | chr4.2:63035824-63035843 | None:intergenic | 45.0% | |
| ! | CTAGCAGATCTTTGGTAGCA+TGG | - | chr4.2:63034652-63034671 | None:intergenic | 45.0% |
| ! | TAAAGAGGTGTAAGACTCGC+GGG | + | chr4.2:63036590-63036609 | MS.gene35546:intron | 45.0% |
| !! | GTGTACGTAATGGGTCTCAT+TGG | + | chr4.2:63036416-63036435 | MS.gene35546:intron | 45.0% |
| !! | AATTATCTTAATATAAATTT+AGG | + | chr4.2:63035456-63035475 | MS.gene35546:intron | 5.0% |
| !!! | TTATTTTTTTTATATTAAAG+AGG | + | chr4.2:63036575-63036594 | MS.gene35546:intron | 5.0% |
| AGGAAGATCAAGTCGAGCCT+GGG | + | chr4.2:63035335-63035354 | MS.gene35546:CDS | 50.0% | |
| CCACTGAAATAGGACTGTGG+AGG | + | chr4.2:63034791-63034810 | MS.gene35546:intron | 50.0% | |
| CCTCCACAGTCCTATTTCAG+TGG | - | chr4.2:63034794-63034813 | None:intergenic | 50.0% | |
| TGTAGCTACCTCTGCCCATA+AGG | - | chr4.2:63034928-63034947 | None:intergenic | 50.0% | |
| TTTCTGCTCCTTATGGGCAG+AGG | + | chr4.2:63034917-63034936 | MS.gene35546:intron | 50.0% | |
| GAGGAAGATCAAGTCGAGCC+TGG | + | chr4.2:63035334-63035353 | MS.gene35546:CDS | 55.0% | |
| TGGGTGGATGCTGGATTTGC+AGG | + | chr4.2:63036750-63036769 | MS.gene35546:CDS | 55.0% | |
| TACTCTGCGTGGGTGGATGC+TGG | + | chr4.2:63036741-63036760 | MS.gene35546:CDS | 60.0% | |
| GGGAGGCTACTCTGCGTGGG+TGG | + | chr4.2:63036734-63036753 | MS.gene35546:CDS | 70.0% | |
| GGGGGGAGGCTACTCTGCGT+GGG | + | chr4.2:63036731-63036750 | MS.gene35546:CDS | 70.0% | |
| TGGGGGGAGGCTACTCTGCG+TGG | + | chr4.2:63036730-63036749 | MS.gene35546:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 63034511 | 63036824 | 63034511 | ID=MS.gene35546 |
| chr4.2 | mRNA | 63034511 | 63036824 | 63034511 | ID=MS.gene35546.t1;Parent=MS.gene35546 |
| chr4.2 | exon | 63034511 | 63034527 | 63034511 | ID=MS.gene35546.t1.exon1;Parent=MS.gene35546.t1 |
| chr4.2 | CDS | 63034511 | 63034527 | 63034511 | ID=cds.MS.gene35546.t1;Parent=MS.gene35546.t1 |
| chr4.2 | exon | 63034622 | 63034702 | 63034622 | ID=MS.gene35546.t1.exon2;Parent=MS.gene35546.t1 |
| chr4.2 | CDS | 63034622 | 63034702 | 63034622 | ID=cds.MS.gene35546.t1;Parent=MS.gene35546.t1 |
| chr4.2 | exon | 63034803 | 63034876 | 63034803 | ID=MS.gene35546.t1.exon3;Parent=MS.gene35546.t1 |
| chr4.2 | CDS | 63034803 | 63034876 | 63034803 | ID=cds.MS.gene35546.t1;Parent=MS.gene35546.t1 |
| chr4.2 | exon | 63035136 | 63035206 | 63035136 | ID=MS.gene35546.t1.exon4;Parent=MS.gene35546.t1 |
| chr4.2 | CDS | 63035136 | 63035206 | 63035136 | ID=cds.MS.gene35546.t1;Parent=MS.gene35546.t1 |
| chr4.2 | exon | 63035321 | 63035374 | 63035321 | ID=MS.gene35546.t1.exon5;Parent=MS.gene35546.t1 |
| chr4.2 | CDS | 63035321 | 63035374 | 63035321 | ID=cds.MS.gene35546.t1;Parent=MS.gene35546.t1 |
| chr4.2 | exon | 63036705 | 63036824 | 63036705 | ID=MS.gene35546.t1.exon6;Parent=MS.gene35546.t1 |
| chr4.2 | CDS | 63036705 | 63036824 | 63036705 | ID=cds.MS.gene35546.t1;Parent=MS.gene35546.t1 |
| Gene Sequence |
| Protein sequence |
