AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061564


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061564.t1 MTR_3g064750 92.000 200 16 0 1 200 75 274 4.18e-137 384
MS.gene061564.t1 MTR_3g064750 82.927 123 18 1 1 123 75 194 1.62e-68 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061564.t1 AT2G01990 37.745 204 96 6 21 200 17 213 7.66e-33 117
MS.gene061564.t1 AT2G01990 37.745 204 96 6 21 200 48 244 2.29e-32 117
MS.gene061564.t1 AT1G14630 37.968 187 101 5 21 200 48 226 8.16e-29 107
MS.gene061564.t1 AT5G48720 41.860 86 35 3 119 200 226 300 1.04e-11 62.8
MS.gene061564.t1 AT5G48720 41.860 86 35 3 119 200 226 300 1.04e-11 62.8
MS.gene061564.t1 AT5G48720 41.860 86 35 3 119 200 192 266 1.23e-11 62.4
MS.gene061564.t1 AT5G48720 41.860 86 35 3 119 200 192 266 1.23e-11 62.4

Find 48 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCGTGGTTATGGTATTTC+AGG 0.166700 3.2:+60262293 MS.gene061564:CDS
TATACATACCTTGAAGTAAT+TGG 0.169435 3.2:-60261565 None:intergenic
CAATCACTGGCTGGGATATT+TGG 0.233908 3.2:+60261333 MS.gene061564:CDS
TTTCCTTTCCTCCATGGAAT+TGG 0.298126 3.2:+60260734 None:intergenic
GAGGCTCCATTACCATTATT+AGG 0.327547 3.2:+60262357 MS.gene061564:CDS
TCAACCTTTAGTCCTAATAA+TGG 0.328259 3.2:-60262369 None:intergenic
TTGAAGTAATTGGGCTGCTT+TGG 0.351121 3.2:-60261555 None:intergenic
GGATGTGGAATTGGCTTTGA+TGG 0.358593 3.2:-60262230 None:intergenic
TGTATTTCAAACCGTGGTTA+TGG 0.360210 3.2:+60262284 MS.gene061564:CDS
ACAGCCACAGGATGTGGAAT+TGG 0.391650 3.2:-60262239 None:intergenic
ACTCATTCAGATTTAACTCT+TGG 0.392699 3.2:+60260768 MS.gene061564:CDS
GAAGAAAATGTTGGAAATAG+AGG 0.396185 3.2:-60261533 None:intergenic
CCAGGTTTCACCAGCTCAAA+TGG 0.402015 3.2:-60262152 None:intergenic
ATGGACTCATCATCATTTGA+TGG 0.411072 3.2:-60262211 None:intergenic
CTAGATGCACAACTTCTTTG+AGG 0.413057 3.2:-60261943 None:intergenic
ATTACCATTATTAGGACTAA+AGG 0.415904 3.2:+60262365 MS.gene061564:CDS
GTTTCCCCTTCAACTCCTCC+AGG 0.422860 3.2:-60262170 None:intergenic
CCATTTGAGCTGGTGAAACC+TGG 0.428678 3.2:+60262152 MS.gene061564:CDS
ATACATACCTTGAAGTAATT+GGG 0.433333 3.2:-60261564 None:intergenic
AAGAAGTGGCAGCACTTACT+AGG 0.434363 3.2:+60262318 MS.gene061564:CDS
TTGAAGATGCAATCACTGGC+TGG 0.441092 3.2:+60261324 MS.gene061564:CDS
TTTAGTCCTAATAATGGTAA+TGG 0.443297 3.2:-60262363 None:intergenic
GTGAAACCTGGAGGAGTTGA+AGG 0.448286 3.2:+60262164 MS.gene061564:CDS
GTGCTTCTACAGCTGAGTCT+TGG 0.458655 3.2:+60261273 MS.gene061564:intron
AACCTACTCTCAAGATCAAA+AGG 0.460972 3.2:+60261376 MS.gene061564:CDS
AAAGCAGCCCAATTACTTCA+AGG 0.465374 3.2:+60261557 MS.gene061564:CDS
TCTTCTACAGCCACAGGATG+TGG 0.472074 3.2:-60262245 None:intergenic
CACTTACTAGGATTCAAACT+AGG 0.484716 3.2:+60262330 MS.gene061564:CDS
GATTCCAATGTCCAATTCCA+TGG 0.514674 3.2:-60260745 None:intergenic
ACTTACTAGGATTCAAACTA+GGG 0.519902 3.2:+60262331 MS.gene061564:CDS
TCCTCCATGGAATTGGACAT+TGG 0.529804 3.2:+60260741 None:intergenic
AGGATTCAAACTAGGGGTAG+AGG 0.539013 3.2:+60262338 MS.gene061564:CDS
TAACCACGGTTTGAAATACA+TGG 0.544046 3.2:-60262281 None:intergenic
GCTGCTTTGGAAGAAAATGT+TGG 0.550147 3.2:-60261542 None:intergenic
TGAAGATGCAATCACTGGCT+GGG 0.560320 3.2:+60261325 MS.gene061564:CDS
AATAGCTTATCCATTTGAGC+TGG 0.561873 3.2:+60262142 MS.gene061564:CDS
ATAGAATCTTCTACAGCCAC+AGG 0.564847 3.2:-60262251 None:intergenic
TGAAACCTGGAGGAGTTGAA+GGG 0.578494 3.2:+60262165 MS.gene061564:CDS
TATCTTGAAGATGCAATCAC+TGG 0.588676 3.2:+60261320 MS.gene061564:CDS
TGGTATTTCAGGAAAAGAAG+TGG 0.597344 3.2:+60262304 MS.gene061564:CDS
CAAAGAAGTTGTGCATCTAG+AGG 0.598019 3.2:+60261946 MS.gene061564:CDS
TTTGCAGCTGATACATCAAG+TGG 0.606270 3.2:+60261296 MS.gene061564:CDS
TTCCTGAAATACCATAACCA+CGG 0.632367 3.2:-60262295 None:intergenic
AAAGCCAATTCCACATCCTG+TGG 0.636787 3.2:+60262235 MS.gene061564:CDS
CATCCATGTATTTCAAACCG+TGG 0.645090 3.2:+60262278 MS.gene061564:CDS
GAAACCTGGAGGAGTTGAAG+GGG 0.660694 3.2:+60262166 MS.gene061564:CDS
TTTGAGCTGGTGAAACCTGG+AGG 0.684329 3.2:+60262155 MS.gene061564:CDS
CTTACTAGGATTCAAACTAG+GGG 0.738551 3.2:+60262332 MS.gene061564:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAAAAAACAAAATGTT+TGG + chr3.2:60261499-60261518 MS.gene061564:intron 10.0%
!!! CAATCATTTGAAAATAATTT+TGG + chr3.2:60261652-60261671 MS.gene061564:intron 15.0%
!!! TAGGTTATTATTTCTAATTT+TGG + chr3.2:60261784-60261803 MS.gene061564:intron 15.0%
!! TAACCTAAAGTTAAAATGTT+AGG - chr3.2:60261771-60261790 None:intergenic 20.0%
!!! ATTATGTTTTGATGTAACTA+GGG + chr3.2:60262105-60262124 MS.gene061564:intron 20.0%
! ACCATAAAAAATTCAACACT+AGG + chr3.2:60261866-60261885 MS.gene061564:CDS 25.0%
! AGAAAAGAGAAATGAAATGA+TGG - chr3.2:60262046-60262065 None:intergenic 25.0%
! ATACATACCTTGAAGTAATT+GGG - chr3.2:60261567-60261586 None:intergenic 25.0%
! ATGAAACAGAGAAGATAAAT+TGG - chr3.2:60261691-60261710 None:intergenic 25.0%
! ATTACCATTATTAGGACTAA+AGG + chr3.2:60262365-60262384 MS.gene061564:CDS 25.0%
! CAATCATGTATTACTAGATT+TGG + chr3.2:60261624-60261643 MS.gene061564:intron 25.0%
! CTAAAGTTAAAATGTTAGGT+TGG - chr3.2:60261767-60261786 None:intergenic 25.0%
! CTAGTTACATCAAAACATAA+TGG - chr3.2:60262106-60262125 None:intergenic 25.0%
! TAATAAATCAGCAAGTGAAA+AGG - chr3.2:60260806-60260825 None:intergenic 25.0%
! TATACATACCTTGAAGTAAT+TGG - chr3.2:60261568-60261587 None:intergenic 25.0%
! TCAAAACATAATGGTACAAA+AGG - chr3.2:60262097-60262116 None:intergenic 25.0%
! TTCAATGTAATCACAAATGT+CGG - chr3.2:60261133-60261152 None:intergenic 25.0%
! TTCTGATTGAAGATATGTTT+CGG + chr3.2:60261071-60261090 MS.gene061564:intron 25.0%
! TTTAGTCCTAATAATGGTAA+TGG - chr3.2:60262366-60262385 None:intergenic 25.0%
!!! CAACCTAACATTTTAACTTT+AGG + chr3.2:60261765-60261784 MS.gene061564:intron 25.0%
!!! CATTATGTTTTGATGTAACT+AGG + chr3.2:60262104-60262123 MS.gene061564:intron 25.0%
!!! TCCTAGTGTTGAATTTTTTA+TGG - chr3.2:60261870-60261889 None:intergenic 25.0%
!!! TTATGTTTTGATGTAACTAG+GGG + chr3.2:60262106-60262125 MS.gene061564:intron 25.0%
ACTCATTCAGATTTAACTCT+TGG + chr3.2:60260768-60260787 MS.gene061564:CDS 30.0%
ACTTACTAGGATTCAAACTA+GGG + chr3.2:60262331-60262350 MS.gene061564:CDS 30.0%
ATATACTACTAGATGACACT+TGG + chr3.2:60260884-60260903 MS.gene061564:intron 30.0%
ATTCTACTTGTTTGTGATCT+TGG + chr3.2:60260922-60260941 MS.gene061564:intron 30.0%
CAAGTAGAATAAGATCATAG+TGG - chr3.2:60260912-60260931 None:intergenic 30.0%
GAAGAAAATGTTGGAAATAG+AGG - chr3.2:60261536-60261555 None:intergenic 30.0%
GATTCAGCAGCTATATAATA+AGG + chr3.2:60261718-60261737 MS.gene061564:intron 30.0%
GTCATCTAGTAGTATATTCT+TGG - chr3.2:60260881-60260900 None:intergenic 30.0%
TCAACCTTTAGTCCTAATAA+TGG - chr3.2:60262372-60262391 None:intergenic 30.0%
TGTATATATATACACCCTTG+TGG + chr3.2:60261582-60261601 MS.gene061564:intron 30.0%
!!! TATGTTTTGATGTAACTAGG+GGG + chr3.2:60262107-60262126 MS.gene061564:intron 30.0%
AACAAAATGTTTGGTTGAGC+AGG + chr3.2:60261508-60261527 MS.gene061564:intron 35.0%
AACCTACTCTCAAGATCAAA+AGG + chr3.2:60261376-60261395 MS.gene061564:CDS 35.0%
AATAGCTTATCCATTTGAGC+TGG + chr3.2:60262142-60262161 MS.gene061564:CDS 35.0%
AATCAGAAATGTCTGCAACA+AGG - chr3.2:60261059-60261078 None:intergenic 35.0%
ATGGACTCATCATCATTTGA+TGG - chr3.2:60262214-60262233 None:intergenic 35.0%
CACTTACTAGGATTCAAACT+AGG + chr3.2:60262330-60262349 MS.gene061564:CDS 35.0%
CTTACTAGGATTCAAACTAG+GGG + chr3.2:60262332-60262351 MS.gene061564:CDS 35.0%
GGTTAACTAACAACTATGCA+TGG + chr3.2:60261397-60261416 MS.gene061564:intron 35.0%
TATCTTGAAGATGCAATCAC+TGG + chr3.2:60261320-60261339 MS.gene061564:CDS 35.0%
TGGTATTTCAGGAAAAGAAG+TGG + chr3.2:60262304-60262323 MS.gene061564:CDS 35.0%
TGTATTTCAAACCGTGGTTA+TGG + chr3.2:60262284-60262303 MS.gene061564:CDS 35.0%
TTCCTGAAATACCATAACCA+CGG - chr3.2:60262298-60262317 None:intergenic 35.0%
! AAAGTAATTTGCAGTGTCAG+TGG - chr3.2:60261839-60261858 None:intergenic 35.0%
! AACCTTTTGATCTTGAGAGT+AGG - chr3.2:60261381-60261400 None:intergenic 35.0%
! GTGACTTTTCTCAATCATGA+TGG - chr3.2:60260990-60261009 None:intergenic 35.0%
! TAACCACGGTTTGAAATACA+TGG - chr3.2:60262284-60262303 None:intergenic 35.0%
! TTTTGATCTTGAGAGTAGGT+TGG - chr3.2:60261377-60261396 None:intergenic 35.0%
!!! GTCTGCTTATTTTGTCTCTA+TGG + chr3.2:60261251-60261270 MS.gene061564:intron 35.0%
AAAGCAGCCCAATTACTTCA+AGG + chr3.2:60261557-60261576 MS.gene061564:CDS 40.0%
AACCGTGGTTATGGTATTTC+AGG + chr3.2:60262293-60262312 MS.gene061564:CDS 40.0%
ATAGAATCTTCTACAGCCAC+AGG - chr3.2:60262254-60262273 None:intergenic 40.0%
ATATACACCCTTGTGGAATG+TGG + chr3.2:60261589-60261608 MS.gene061564:intron 40.0%
CAAAGAAGTTGTGCATCTAG+AGG + chr3.2:60261946-60261965 MS.gene061564:CDS 40.0%
CATCCATGTATTTCAAACCG+TGG + chr3.2:60262278-60262297 MS.gene061564:CDS 40.0%
CTAGATGCACAACTTCTTTG+AGG - chr3.2:60261946-60261965 None:intergenic 40.0%
GAGGCTCCATTACCATTATT+AGG + chr3.2:60262357-60262376 MS.gene061564:CDS 40.0%
TCACACTTTGAACCTTCTGA+CGG + chr3.2:60261006-60261025 MS.gene061564:intron 40.0%
TTGAAGTAATTGGGCTGCTT+TGG - chr3.2:60261558-60261577 None:intergenic 40.0%
TTTGCAGCTGATACATCAAG+TGG + chr3.2:60261296-60261315 MS.gene061564:CDS 40.0%
!! GCTGCTTTGGAAGAAAATGT+TGG - chr3.2:60261545-60261564 None:intergenic 40.0%
AAAGCCAATTCCACATCCTG+TGG + chr3.2:60262235-60262254 MS.gene061564:CDS 45.0%
AAGAAGTGGCAGCACTTACT+AGG + chr3.2:60262318-60262337 MS.gene061564:CDS 45.0%
AGGATTCAAACTAGGGGTAG+AGG + chr3.2:60262338-60262357 MS.gene061564:CDS 45.0%
CAGCAAACACAACACTGACA+CGG - chr3.2:60261198-60261217 None:intergenic 45.0%
GATGCAACCACATTCCACAA+GGG - chr3.2:60261599-60261618 None:intergenic 45.0%
GTCTCTATGAAGCACAGACA+CGG - chr3.2:60261222-60261241 None:intergenic 45.0%
TCTGCAACAAGGACTGAGTT+TGG - chr3.2:60261048-60261067 None:intergenic 45.0%
TGAAACCTGGAGGAGTTGAA+GGG + chr3.2:60262165-60262184 MS.gene061564:CDS 45.0%
TGATGCAACCACATTCCACA+AGG - chr3.2:60261600-60261619 None:intergenic 45.0%
TTGAAGATGCAATCACTGGC+TGG + chr3.2:60261324-60261343 MS.gene061564:CDS 45.0%
! CAATCACTGGCTGGGATATT+TGG + chr3.2:60261333-60261352 MS.gene061564:CDS 45.0%
! GTAATTTGCAGTGTCAGTGG+TGG - chr3.2:60261836-60261855 None:intergenic 45.0%
! TGAAGATGCAATCACTGGCT+GGG + chr3.2:60261325-60261344 MS.gene061564:CDS 45.0%
!! GGATGTGGAATTGGCTTTGA+TGG - chr3.2:60262233-60262252 None:intergenic 45.0%
ACAGCCACAGGATGTGGAAT+TGG - chr3.2:60262242-60262261 None:intergenic 50.0%
ATCACGACAACACCGTCAGA+AGG - chr3.2:60261021-60261040 None:intergenic 50.0%
CCAGGTTTCACCAGCTCAAA+TGG - chr3.2:60262155-60262174 None:intergenic 50.0%
CCATTTGAGCTGGTGAAACC+TGG + chr3.2:60262152-60262171 MS.gene061564:CDS 50.0%
GAAACCTGGAGGAGTTGAAG+GGG + chr3.2:60262166-60262185 MS.gene061564:CDS 50.0%
GTGAAACCTGGAGGAGTTGA+AGG + chr3.2:60262164-60262183 MS.gene061564:CDS 50.0%
GTGCTTCTACAGCTGAGTCT+TGG + chr3.2:60261273-60261292 MS.gene061564:intron 50.0%
TCTTCTACAGCCACAGGATG+TGG - chr3.2:60262248-60262267 None:intergenic 50.0%
TGATGTAACTAGGGGGACAG+TGG + chr3.2:60262114-60262133 MS.gene061564:intron 50.0%
TTTGAGCTGGTGAAACCTGG+AGG + chr3.2:60262155-60262174 MS.gene061564:CDS 50.0%
CTGACACGGACACGTTGACA+CGG - chr3.2:60261184-60261203 None:intergenic 55.0%
GTTTCCCCTTCAACTCCTCC+AGG - chr3.2:60262173-60262192 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 60260747 60262391 60260747 ID=MS.gene061564
chr3.2 mRNA 60260747 60262391 60260747 ID=MS.gene061564.t1;Parent=MS.gene061564
chr3.2 exon 60260747 60260789 60260747 ID=MS.gene061564.t1.exon1;Parent=MS.gene061564.t1
chr3.2 CDS 60260747 60260789 60260747 ID=cds.MS.gene061564.t1;Parent=MS.gene061564.t1
chr3.2 exon 60261285 60261397 60261285 ID=MS.gene061564.t1.exon2;Parent=MS.gene061564.t1
chr3.2 CDS 60261285 60261397 60261285 ID=cds.MS.gene061564.t1;Parent=MS.gene061564.t1
chr3.2 exon 60261530 60261578 60261530 ID=MS.gene061564.t1.exon3;Parent=MS.gene061564.t1
chr3.2 CDS 60261530 60261578 60261530 ID=cds.MS.gene061564.t1;Parent=MS.gene061564.t1
chr3.2 exon 60261866 60261997 60261866 ID=MS.gene061564.t1.exon4;Parent=MS.gene061564.t1
chr3.2 CDS 60261866 60261997 60261866 ID=cds.MS.gene061564.t1;Parent=MS.gene061564.t1
chr3.2 exon 60262126 60262391 60262126 ID=MS.gene061564.t1.exon5;Parent=MS.gene061564.t1
chr3.2 CDS 60262126 60262391 60262126 ID=cds.MS.gene061564.t1;Parent=MS.gene061564.t1
Gene Sequence

>MS.gene061564

ATGGAATTGGACATTGGAATCACTCATTCAGATTTAACTCTTGGTATTTTTGCTATCCTTTTCACTTGCTGATTTATTATTTATCTTTATTTTTTTCATTTTCATTTTCATTTTCTTTGATTCACTTGTTACCAAGAATATACTACTAGATGACACTTGGCACCACTATGATCTTATTCTACTTGTTTGTGATCTTGGTCTATATTTAGATTGACAGTGAATCTAGTAGAATCACATTTGCCATCATGATTGAGAAAAGTCACACTTTGAACCTTCTGACGGTGTTGTCGTGATTTTGCCAAACTCAGTCCTTGTTGCAGACATTTCTGATTGAAGATATGTTTCGGTATCAGACACTTATCGTGTTTGATATCGACACTACACCGACATTTGTGATTACATTGAATTATGTTATTTAAAATATAAAAAAAAAACCGTGTCAACGTGTCCGTGTCAGTGTTGTGTTTGCTGTCCGTGTCTGTGCTTCATAGAGACTCACTGTATGTCTGCTTATTTTGTCTCTATGGTGCTTCTACAGCTGAGTCTTGGTTTGCAGCTGATACATCAAGTGGATATCTTGAAGATGCAATCACTGGCTGGGATATTTGGTGCAAGCAAAATAACTTACCAACCTACTCTCAAGATCAAAAGGTTAACTAACAACTATGCATGGTTTCATGTTCTTTACATTTGCTTAATTGTTATTTCATAGTTTTTATTTGTCAAATATGTTGAAACTTTATAAGATATATTAATAAAAAAACAAAATGTTTGGTTGAGCAGGAACCTCTATTTCCAACATTTTCTTCCAAAGCAGCCCAATTACTTCAAGGTATGTATATATATACACCCTTGTGGAATGTGGTTGCATCACTTTCAATCATGTATTACTAGATTTGGAAAATCAATCATTTGAAAATAATTTTGGTTATTTATCTAGTCCAATTTATCTTCTCTGTTTCATGTTTACTGATTCAGCAGCTATATAATAAGGAGTTAAGAATTTGCAATAAATGACCAACCTAACATTTTAACTTTAGGTTATTATTTCTAATTTTGGTAGTATTGAAAGTAATTGAACTATATCCACCACTGACACTGCAAATTACTTTTTTACAGACCATAAAAAATTCAACACTAGGAATCTATCATCATCACAAGATCATACACATTCAGCTGCAGTAAATCATGATTCACCTCAAAGAAGTTGTGCATCTAGAGGTTCAAATGAAAATGTTGCTTCTGTTTCAAGTACAAAATTCTTTTACTGTTTTAACACAAGAACATTTTCTTGAACCATCATTTCATTTCTCTTTTCTAAAGTAATTTTTTATGACTAAAATGTGTCCTTTTGTACCATTATGTTTTGATGTAACTAGGGGGACAGTGGAAGAAAATAGCTTATCCATTTGAGCTGGTGAAACCTGGAGGAGTTGAAGGGGAAACAACAATTAAAGATATAAACCATCAAATGATGATGAGTCCATCAAAGCCAATTCCACATCCTGTGGCTGTAGAAGATTCTATGACTCATCCATGTATTTCAAACCGTGGTTATGGTATTTCAGGAAAAGAAGTGGCAGCACTTACTAGGATTCAAACTAGGGGTAGAGGCTCCATTACCATTATTAGGACTAAAGGTTGA

Protein sequence

>MS.gene061564.t1

MELDIGITHSDLTLAESWFAADTSSGYLEDAITGWDIWCKQNNLPTYSQDQKEPLFPTFSSKAAQLLQDHKKFNTRNLSSSQDHTHSAAVNHDSPQRSCASRGSNENVASVSRGQWKKIAYPFELVKPGGVEGETTIKDINHQMMMSPSKPIPHPVAVEDSMTHPCISNRGYGISGKEVAALTRIQTRGRGSITIIRTKG