AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20457

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20457.t1 MTR_6g011640 92.519 401 27 1 27 427 1 398 0.0 759
MS.gene20457.t1 MTR_7g010090 51.368 475 141 15 1 427 1 433 7.29e-128 376
MS.gene20457.t1 MTR_3g030520 85.625 160 23 0 268 427 141 300 2.05e-85 263
MS.gene20457.t1 MTR_5g048870 81.529 157 27 2 196 350 1 157 7.86e-69 215
MS.gene20457.t1 MTR_7g079015 87.255 102 13 0 302 403 1 102 1.84e-55 179
MS.gene20457.t1 MTR_1g095960 84.848 99 15 0 306 404 32 130 1.95e-51 169
MS.gene20457.t1 MTR_1g053325 81.553 103 19 0 302 404 1 103 1.17e-50 166
MS.gene20457.t1 MTR_2g026830 80.583 103 16 1 302 404 1 99 1.59e-49 163
MS.gene20457.t1 MTR_1g098230 85.263 95 14 0 310 404 9 103 6.18e-49 162
MS.gene20457.t1 MTR_2g028040 81.818 110 17 2 302 410 1 108 9.04e-49 170
MS.gene20457.t1 MTR_2g028040 83.582 67 11 0 337 403 290 356 4.83e-27 111
MS.gene20457.t1 MTR_2g028040 80.303 66 13 0 338 403 225 290 3.39e-24 103
MS.gene20457.t1 MTR_2g028040 88.710 62 7 0 341 402 167 228 1.11e-17 84.3
MS.gene20457.t1 MTR_2g028040 83.871 62 10 0 334 395 103 164 4.06e-16 79.7
MS.gene20457.t1 MTR_5g088530 71.795 117 25 2 288 404 1 109 1.53e-46 156
MS.gene20457.t1 MTR_1g051180 31.278 454 194 15 1 426 1 364 4.64e-46 163
MS.gene20457.t1 MTR_5g065050 84.466 103 16 0 302 404 1 103 2.05e-45 153
MS.gene20457.t1 MTR_7g078370 67.857 112 33 1 290 398 34 145 4.25e-42 146
MS.gene20457.t1 MTR_5g031560 62.698 126 13 1 302 427 1 92 4.30e-42 144
MS.gene20457.t1 MTR_5g064740 74.757 103 14 1 302 404 1 91 1.13e-41 142
MS.gene20457.t1 MTR_7g090230 29.322 457 201 14 15 426 46 425 7.21e-41 150
MS.gene20457.t1 MTR_6g042350 70.588 102 15 1 302 403 1 87 9.50e-39 135
MS.gene20457.t1 MTR_2g436640 86.301 73 10 0 332 404 60 132 3.15e-37 134
MS.gene20457.t1 MTR_5g083110 43.275 171 77 4 257 427 90 240 2.87e-36 134
MS.gene20457.t1 MTR_4g109990 58.065 124 8 1 286 409 1 80 1.78e-35 126
MS.gene20457.t1 MTR_3g017490 42.105 171 81 4 255 425 108 260 3.64e-35 132
MS.gene20457.t1 MTR_3g465550 44.720 161 70 3 261 421 87 228 1.23e-34 129
MS.gene20457.t1 MTR_8g038440 42.857 168 77 4 262 427 106 256 3.05e-34 128
MS.gene20457.t1 MTR_7g104720 43.558 163 72 3 264 426 263 405 1.34e-33 130
MS.gene20457.t1 MTR_3g071510 42.073 164 75 4 264 427 93 236 3.96e-33 125
MS.gene20457.t1 MTR_5g072400 40.120 167 76 4 261 425 109 253 1.24e-31 121
MS.gene20457.t1 MTR_7g086680 77.215 79 15 1 302 380 1 76 6.61e-31 114
MS.gene20457.t1 MTR_7g106500 38.824 170 93 4 257 426 121 279 4.53e-30 117
MS.gene20457.t1 MTR_1g073880 61.165 103 12 1 302 404 1 75 3.19e-29 109
MS.gene20457.t1 MTR_1g090400 35.321 218 113 6 216 425 171 368 6.20e-28 114
MS.gene20457.t1 MTR_8g099665 35.922 206 116 6 223 425 175 367 3.50e-27 112
MS.gene20457.t1 MTR_4g059510 64.286 98 35 0 307 404 39 136 1.77e-25 101
MS.gene20457.t1 MTR_6g086570 88.235 68 8 0 337 404 19 86 2.53e-25 99.4
MS.gene20457.t1 MTR_8g106900 39.535 172 91 3 254 425 115 273 9.52e-25 103
MS.gene20457.t1 MTR_0140s0020 84.746 59 9 0 302 360 1 59 5.23e-21 86.3
MS.gene20457.t1 MTR_5g061260 77.027 74 15 1 339 412 2 73 3.67e-20 84.7
MS.gene20457.t1 MTR_4g088575 74.545 55 13 1 313 366 15 69 6.95e-19 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20457.t1 AT5G39865 46.925 439 170 14 1 426 1 389 4.66e-113 337
MS.gene20457.t1 AT3G28850 46.405 459 181 16 1 426 1 427 5.42e-95 292
MS.gene20457.t1 AT2G41330 31.739 460 180 16 15 427 30 402 4.52e-51 177
MS.gene20457.t1 AT3G57070 35.000 360 156 15 117 427 87 417 4.74e-47 167
MS.gene20457.t1 AT5G58530 41.176 170 77 3 264 427 115 267 2.40e-36 134
MS.gene20457.t1 AT5G13810 43.114 167 73 5 261 425 126 272 1.19e-35 132
MS.gene20457.t1 AT1G64500 39.759 166 86 5 262 425 212 365 1.12e-31 124
MS.gene20457.t1 AT5G06470 39.053 169 89 3 257 425 84 238 2.01e-29 115
MS.gene20457.t1 AT4G10630 32.372 312 160 11 122 425 65 333 2.73e-29 117
MS.gene20457.t1 AT5G03870 27.972 429 205 14 35 425 21 383 8.89e-27 111
MS.gene20457.t1 AT1G32760 40.491 163 86 4 263 425 162 313 5.43e-25 104
MS.gene20457.t1 AT3G11773 36.875 160 80 3 266 425 11 149 2.38e-24 98.6
MS.gene20457.t1 AT5G01420 33.728 169 93 4 257 425 251 400 3.21e-18 86.7
MS.gene20457.t1 AT3G11773 34.884 129 63 3 297 425 92 199 4.05e-16 77.0

Find 80 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACAACATTAGGTTCTT+TGG 0.176849 6.3:+6314200 MS.gene20457:CDS
GGTTTCTTGGCAATAGATTT+TGG 0.183871 6.3:-6314423 None:intergenic
GAGAAGCTTCTTAATTGATT+TGG 0.246490 6.3:-6314528 None:intergenic
GGATTACCAAAGGTCTTTAT+TGG 0.262282 6.3:+6315029 MS.gene20457:CDS
TCCTTCAAAGCCTAGTTTCA+TGG 0.285192 6.3:+6314581 MS.gene20457:CDS
TTACTTAGTGAAGGGAATTA+TGG 0.296817 6.3:+6314999 MS.gene20457:CDS
ATGAGGATTGTTGCAAAGTT+AGG 0.312147 6.3:+6314895 MS.gene20457:CDS
TAGGCTTTGAAGGATTAAAA+TGG 0.314617 6.3:-6314572 None:intergenic
TGGTGTTGTGATTGGTTTCT+TGG 0.318370 6.3:-6314436 None:intergenic
CCCTCTTGCTTCTCTTGCAA+TGG 0.331746 6.3:-6314738 None:intergenic
CATCAACACATGGGAACTAA+TGG 0.354432 6.3:+6314473 MS.gene20457:CDS
CATTGCAATGAGGACACCTT+TGG 0.363064 6.3:-6315286 None:intergenic
CTTCTAGAGACAGAACAATA+AGG 0.364778 6.3:-6314111 None:intergenic
GCATTGACATCAACAACATT+AGG 0.382700 6.3:+6314192 MS.gene20457:CDS
AAGAGATGTGTCAATGCATT+TGG 0.388000 6.3:+6314953 MS.gene20457:CDS
TTCCTTCCAATAAAGACCTT+TGG 0.389330 6.3:-6315035 None:intergenic
TGTCCTCATTGCAATGAAAA+TGG 0.389430 6.3:+6315293 MS.gene20457:CDS
TTCTAGAGACAGAACAATAA+GGG 0.389787 6.3:-6314110 None:intergenic
TCACAAGGTGGTGTTGTGAT+TGG 0.390904 6.3:-6314444 None:intergenic
TCCATGAAACTAGGCTTTGA+AGG 0.397792 6.3:-6314582 None:intergenic
GATGACATTGAAGGAGTTGA+TGG 0.405538 6.3:+6315140 MS.gene20457:CDS
AATTGATTTGGTGATTGAAA+AGG 0.420860 6.3:-6314516 None:intergenic
GAAGATGATGATTGTGAGCT+TGG 0.425710 6.3:+6315251 MS.gene20457:CDS
GCTTTGAGCAGAGTTGAATA+TGG 0.429778 6.3:-6314258 None:intergenic
TTGTGTGAGTTCCAAGCAAA+AGG 0.433319 6.3:+6314068 MS.gene20457:CDS
TGATTTACACAAACCAAAGT+TGG 0.433774 6.3:+6314620 MS.gene20457:CDS
GTTCCTTGTGAAACATGTTA+TGG 0.450625 6.3:+6315194 MS.gene20457:CDS
AACGGATTTGAGGTCGGAAA+AGG 0.456537 6.3:+6314306 MS.gene20457:CDS
ATAATCATATGATCCAACTT+TGG 0.462563 6.3:-6314633 None:intergenic
TTTGAAAGGATTAGGCATAA+GGG 0.466484 6.3:+6314923 MS.gene20457:CDS
AGAGATGTGTCAATGCATTT+GGG 0.468333 6.3:+6314954 MS.gene20457:CDS
GTTTGCCCCTTATGAGAAGA+AGG 0.471321 6.3:+6314824 MS.gene20457:CDS
TAGTGAGAATTTCAAAGAAG+AGG 0.488318 6.3:+6314353 MS.gene20457:CDS
AAAGCAATCCAACGGATTTG+AGG 0.493472 6.3:+6314296 MS.gene20457:CDS
TACCAAAGGTCTTTATTGGA+AGG 0.497940 6.3:+6315033 MS.gene20457:CDS
CTTAAAGAGTTACTTAGTGA+AGG 0.500503 6.3:+6314990 MS.gene20457:CDS
GTTGGATCATATGATTATTG+AGG 0.501173 6.3:+6314638 MS.gene20457:CDS
ATTCTCACTAACCTTACCAT+TGG 0.518981 6.3:-6314340 None:intergenic
ATGAAAATGAGGCGAAGACA+TGG 0.519341 6.3:+6314376 MS.gene20457:CDS
GATTGTGAGCTTGGAGAGTG+TGG 0.523118 6.3:+6315260 MS.gene20457:CDS
TCATCATTCTCCATGAAACT+AGG 0.532643 6.3:-6314591 None:intergenic
CAAAGAAGAGGATGAAAATG+AGG 0.536996 6.3:+6314365 MS.gene20457:CDS
TTAAAGAGTTACTTAGTGAA+GGG 0.537651 6.3:+6314991 MS.gene20457:CDS
AGTTGTAAAATCTACTATGA+AGG 0.542921 6.3:+6315218 MS.gene20457:CDS
GCAAGAGAAGCAAGAGGGGA+TGG 0.552418 6.3:+6314743 MS.gene20457:CDS
AGTGAAGGGAATTATGGTAA+AGG 0.553388 6.3:+6315005 MS.gene20457:CDS
GACATGGTCTAATATGATTG+AGG 0.555260 6.3:+6314392 MS.gene20457:CDS
GTATTTCACAAGCCTACGTA+TGG 0.555287 6.3:+6314860 MS.gene20457:CDS
TTTATTGGAAGGAAATACAT+TGG 0.560125 6.3:+6315044 MS.gene20457:CDS
ATCAAATGACTTAAATCTCA+AGG 0.561803 6.3:+6314665 MS.gene20457:CDS
GAACTTGTATCTCAAAGTCA+AGG 0.563359 6.3:+6314222 MS.gene20457:CDS
GAAGGGAATTATGGTAAAGG+AGG 0.573000 6.3:+6315008 MS.gene20457:CDS
GTATCTCAAAGTCAAGGCAA+TGG 0.579991 6.3:+6314228 MS.gene20457:CDS
AGTTTCATGGAGAATGATGA+TGG 0.580432 6.3:+6314594 MS.gene20457:CDS
CATTGCAAGAGAAGCAAGAG+GGG 0.583235 6.3:+6314739 MS.gene20457:CDS
GAGAGGATTGATGACATTGA+AGG 0.583825 6.3:+6315131 MS.gene20457:CDS
AATCACAACACCACCTTGTG+AGG 0.588710 6.3:+6314446 MS.gene20457:CDS
TGTGTGAGTTCCAAGCAAAA+GGG 0.588882 6.3:+6314069 MS.gene20457:CDS
ATTGGAAGGAAATACATTGG+TGG 0.594677 6.3:+6315047 MS.gene20457:CDS
ACCTCAGAAAAGCAATCCAA+CGG 0.599597 6.3:+6314288 MS.gene20457:CDS
CTTCCATAACATGTTTCACA+AGG 0.605452 6.3:-6315197 None:intergenic
GATGGTGGATGTGAGGCTTG+TGG 0.611737 6.3:+6315158 MS.gene20457:CDS
AGGAGTTGATGGTGGATGTG+AGG 0.615164 6.3:+6315151 MS.gene20457:CDS
GACATTGAAGGAGTTGATGG+TGG 0.618216 6.3:+6315143 MS.gene20457:CDS
AATTGTTTGATTGTTGTGAG+AGG 0.619018 6.3:+6315114 MS.gene20457:CDS
CCATTGCAAGAGAAGCAAGA+GGG 0.622568 6.3:+6314738 MS.gene20457:CDS
GTGAGGACATCATCAACACA+TGG 0.623721 6.3:+6314463 MS.gene20457:CDS
GATCATATGATTATTGAGGA+CGG 0.631472 6.3:+6314642 MS.gene20457:CDS
CAATCCAACGGATTTGAGGT+CGG 0.636399 6.3:+6314300 MS.gene20457:CDS
TTGGAGAGTGTGGATTCCAA+AGG 0.640413 6.3:+6315270 MS.gene20457:CDS
CATCATCCACCTCAAAACAA+AGG 0.648374 6.3:+6314150 MS.gene20457:CDS
AACACATGGGAACTAATGGA+AGG 0.650905 6.3:+6314477 MS.gene20457:CDS
GCCATTGCAAGAGAAGCAAG+AGG 0.651463 6.3:+6314737 MS.gene20457:CDS
TCTAGAGACAGAACAATAAG+GGG 0.660252 6.3:-6314109 None:intergenic
TGGTAAAGGAGGATTACCAA+AGG 0.683497 6.3:+6315019 MS.gene20457:CDS
ATATTCAACTCTGCTCAAAG+CGG 0.696374 6.3:+6314260 MS.gene20457:CDS
GTGTTGATGATGTCCTCACA+AGG 0.697785 6.3:-6314459 None:intergenic
TGAGGACATCATCAACACAT+GGG 0.714205 6.3:+6314464 MS.gene20457:CDS
TTGATGATGTCCTCACAAGG+TGG 0.717699 6.3:-6314456 None:intergenic
ATAGAACTAGAAACAACACA+TGG 0.736067 6.3:-6315330 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACAAAAAGATTCTTTTTTG+AGG + chr6.3:6314767-6314786 MS.gene20457:CDS 20.0%
! AGTTGTAAAATCTACTATGA+AGG + chr6.3:6315218-6315237 MS.gene20457:CDS 25.0%
! ATAATCATATGATCCAACTT+TGG - chr6.3:6314636-6314655 None:intergenic 25.0%
! ATCAAATGACTTAAATCTCA+AGG + chr6.3:6314665-6314684 MS.gene20457:CDS 25.0%
! TTAAAGAGTTACTTAGTGAA+GGG + chr6.3:6314991-6315010 MS.gene20457:CDS 25.0%
! TTTATTGGAAGGAAATACAT+TGG + chr6.3:6315044-6315063 MS.gene20457:CDS 25.0%
!! AAAGTTAGGATGATTTTGAA+AGG + chr6.3:6314909-6314928 MS.gene20457:CDS 25.0%
!! TCTTTTGATGTTAATGTCAA+TGG + chr6.3:6314549-6314568 MS.gene20457:CDS 25.0%
!!! AATTGATTTGGTGATTGAAA+AGG - chr6.3:6314519-6314538 None:intergenic 25.0%
!!! AGATTTTGATGATTGTAAAG+TGG + chr6.3:6314698-6314717 MS.gene20457:CDS 25.0%
AATTGTTTGATTGTTGTGAG+AGG + chr6.3:6315114-6315133 MS.gene20457:CDS 30.0%
ATCAACAACATTAGGTTCTT+TGG + chr6.3:6314200-6314219 MS.gene20457:CDS 30.0%
CTTAAAGAGTTACTTAGTGA+AGG + chr6.3:6314990-6315009 MS.gene20457:CDS 30.0%
GAGAAGCTTCTTAATTGATT+TGG - chr6.3:6314531-6314550 None:intergenic 30.0%
GATCATATGATTATTGAGGA+CGG + chr6.3:6314642-6314661 MS.gene20457:CDS 30.0%
GTTGGATCATATGATTATTG+AGG + chr6.3:6314638-6314657 MS.gene20457:CDS 30.0%
TAGTGAGAATTTCAAAGAAG+AGG + chr6.3:6314353-6314372 MS.gene20457:CDS 30.0%
TATGGTGAGAAAAACTTATG+AGG + chr6.3:6314878-6314897 MS.gene20457:CDS 30.0%
TGATTTACACAAACCAAAGT+TGG + chr6.3:6314620-6314639 MS.gene20457:CDS 30.0%
TTACTTAGTGAAGGGAATTA+TGG + chr6.3:6314999-6315018 MS.gene20457:CDS 30.0%
TTTGAAAGGATTAGGCATAA+GGG + chr6.3:6314923-6314942 MS.gene20457:CDS 30.0%
! CTTTTTCCTTCTTCTCATAA+GGG - chr6.3:6314833-6314852 None:intergenic 30.0%
! TTCTAGAGACAGAACAATAA+GGG - chr6.3:6314113-6314132 None:intergenic 30.0%
! TTTTTCCTTCTTCTCATAAG+GGG - chr6.3:6314832-6314851 None:intergenic 30.0%
!! ACTTTTTCCTTCTTCTCATA+AGG - chr6.3:6314834-6314853 None:intergenic 30.0%
!! AGGATGATTTTGAAAGGATT+AGG + chr6.3:6314915-6314934 MS.gene20457:CDS 30.0%
!! TAGGCTTTGAAGGATTAAAA+TGG - chr6.3:6314575-6314594 None:intergenic 30.0%
!! TTTTGAAAGGATTAGGCATA+AGG + chr6.3:6314922-6314941 MS.gene20457:CDS 30.0%
!!! TAACTATCTCCTTTGTTTTG+AGG - chr6.3:6314162-6314181 None:intergenic 30.0%
AAGAGATGTGTCAATGCATT+TGG + chr6.3:6314953-6314972 MS.gene20457:CDS 35.0%
AGAGATGTGTCAATGCATTT+GGG + chr6.3:6314954-6314973 MS.gene20457:CDS 35.0%
AGTGAAGGGAATTATGGTAA+AGG + chr6.3:6315005-6315024 MS.gene20457:CDS 35.0%
AGTTTCATGGAGAATGATGA+TGG + chr6.3:6314594-6314613 MS.gene20457:CDS 35.0%
ATATTCAACTCTGCTCAAAG+CGG + chr6.3:6314260-6314279 MS.gene20457:CDS 35.0%
ATGAGGATTGTTGCAAAGTT+AGG + chr6.3:6314895-6314914 MS.gene20457:CDS 35.0%
ATTCTCACTAACCTTACCAT+TGG - chr6.3:6314343-6314362 None:intergenic 35.0%
ATTGGAAGGAAATACATTGG+TGG + chr6.3:6315047-6315066 MS.gene20457:CDS 35.0%
CAAAGAAGAGGATGAAAATG+AGG + chr6.3:6314365-6314384 MS.gene20457:CDS 35.0%
CTTCCATAACATGTTTCACA+AGG - chr6.3:6315200-6315219 None:intergenic 35.0%
GAACTTGTATCTCAAAGTCA+AGG + chr6.3:6314222-6314241 MS.gene20457:CDS 35.0%
GACATGGTCTAATATGATTG+AGG + chr6.3:6314392-6314411 MS.gene20457:CDS 35.0%
GGATTACCAAAGGTCTTTAT+TGG + chr6.3:6315029-6315048 MS.gene20457:CDS 35.0%
GTTCCTTGTGAAACATGTTA+TGG + chr6.3:6315194-6315213 MS.gene20457:CDS 35.0%
TACCAAAGGTCTTTATTGGA+AGG + chr6.3:6315033-6315052 MS.gene20457:CDS 35.0%
TCATCATTCTCCATGAAACT+AGG - chr6.3:6314594-6314613 None:intergenic 35.0%
TCTAGAGACAGAACAATAAG+GGG - chr6.3:6314112-6314131 None:intergenic 35.0%
TGTCCTCATTGCAATGAAAA+TGG + chr6.3:6315293-6315312 MS.gene20457:CDS 35.0%
TTCCTTCCAATAAAGACCTT+TGG - chr6.3:6315038-6315057 None:intergenic 35.0%
! AAAGGTTTCGTGTTTTCCAA+TGG + chr6.3:6314324-6314343 MS.gene20457:CDS 35.0%
! AAGTTTTTCTCACCATACGT+AGG - chr6.3:6314875-6314894 None:intergenic 35.0%
! AGACCATTTTCATTGCAATG+AGG - chr6.3:6315299-6315318 None:intergenic 35.0%
! ATGACAATATCCCTTTTGCT+TGG - chr6.3:6314082-6314101 None:intergenic 35.0%
! CTTCTAGAGACAGAACAATA+AGG - chr6.3:6314114-6314133 None:intergenic 35.0%
! GCATTGACATCAACAACATT+AGG + chr6.3:6314192-6314211 MS.gene20457:CDS 35.0%
! GGTTTCTTGGCAATAGATTT+TGG - chr6.3:6314426-6314445 None:intergenic 35.0%
! TTTCGTGTTTTCCAATGGTA+AGG + chr6.3:6314329-6314348 MS.gene20457:CDS 35.0%
AACACATGGGAACTAATGGA+AGG + chr6.3:6314477-6314496 MS.gene20457:CDS 40.0%
ACCTCAGAAAAGCAATCCAA+CGG + chr6.3:6314288-6314307 MS.gene20457:CDS 40.0%
ATGAAAATGAGGCGAAGACA+TGG + chr6.3:6314376-6314395 MS.gene20457:CDS 40.0%
CATCAACACATGGGAACTAA+TGG + chr6.3:6314473-6314492 MS.gene20457:CDS 40.0%
CATCATCCACCTCAAAACAA+AGG + chr6.3:6314150-6314169 MS.gene20457:CDS 40.0%
GAAGATGATGATTGTGAGCT+TGG + chr6.3:6315251-6315270 MS.gene20457:CDS 40.0%
GAAGGGAATTATGGTAAAGG+AGG + chr6.3:6315008-6315027 MS.gene20457:CDS 40.0%
GATGACATTGAAGGAGTTGA+TGG + chr6.3:6315140-6315159 MS.gene20457:CDS 40.0%
GTATCTCAAAGTCAAGGCAA+TGG + chr6.3:6314228-6314247 MS.gene20457:CDS 40.0%
GTATTTCACAAGCCTACGTA+TGG + chr6.3:6314860-6314879 MS.gene20457:CDS 40.0%
TCCTTCAAAGCCTAGTTTCA+TGG + chr6.3:6314581-6314600 MS.gene20457:CDS 40.0%
TGAGGACATCATCAACACAT+GGG + chr6.3:6314464-6314483 MS.gene20457:CDS 40.0%
TGGTAAAGGAGGATTACCAA+AGG + chr6.3:6315019-6315038 MS.gene20457:CDS 40.0%
TGTGTGAGTTCCAAGCAAAA+GGG + chr6.3:6314069-6314088 MS.gene20457:CDS 40.0%
TTGTGTGAGTTCCAAGCAAA+AGG + chr6.3:6314068-6314087 MS.gene20457:CDS 40.0%
! AAAGCAATCCAACGGATTTG+AGG + chr6.3:6314296-6314315 MS.gene20457:CDS 40.0%
! GAGAGGATTGATGACATTGA+AGG + chr6.3:6315131-6315150 MS.gene20457:CDS 40.0%
! GCTTTGAGCAGAGTTGAATA+TGG - chr6.3:6314261-6314280 None:intergenic 40.0%
! TCCATGAAACTAGGCTTTGA+AGG - chr6.3:6314585-6314604 None:intergenic 40.0%
!! TGGTGTTGTGATTGGTTTCT+TGG - chr6.3:6314439-6314458 None:intergenic 40.0%
!!! CTATCTCCTTTGTTTTGAGG+TGG - chr6.3:6314159-6314178 None:intergenic 40.0%
AATCACAACACCACCTTGTG+AGG + chr6.3:6314446-6314465 MS.gene20457:CDS 45.0%
CATTGCAAGAGAAGCAAGAG+GGG + chr6.3:6314739-6314758 MS.gene20457:CDS 45.0%
CATTGCAATGAGGACACCTT+TGG - chr6.3:6315289-6315308 None:intergenic 45.0%
CCATTGCAAGAGAAGCAAGA+GGG + chr6.3:6314738-6314757 MS.gene20457:CDS 45.0%
GTGAGGACATCATCAACACA+TGG + chr6.3:6314463-6314482 MS.gene20457:CDS 45.0%
GTTTGCCCCTTATGAGAAGA+AGG + chr6.3:6314824-6314843 MS.gene20457:CDS 45.0%
! AACGGATTTGAGGTCGGAAA+AGG + chr6.3:6314306-6314325 MS.gene20457:CDS 45.0%
! CAATCCAACGGATTTGAGGT+CGG + chr6.3:6314300-6314319 MS.gene20457:CDS 45.0%
! TTGGAGAGTGTGGATTCCAA+AGG + chr6.3:6315270-6315289 MS.gene20457:CDS 45.0%
! TTTTCCGACCTCAAATCCGT+TGG - chr6.3:6314307-6314326 None:intergenic 45.0%
!! GACATTGAAGGAGTTGATGG+TGG + chr6.3:6315143-6315162 MS.gene20457:CDS 45.0%
!! GTGTTGATGATGTCCTCACA+AGG - chr6.3:6314462-6314481 None:intergenic 45.0%
!! TCACAAGGTGGTGTTGTGAT+TGG - chr6.3:6314447-6314466 None:intergenic 45.0%
!! TCCGTTGGATTGCTTTTCTG+AGG - chr6.3:6314292-6314311 None:intergenic 45.0%
!! TTGATGATGTCCTCACAAGG+TGG - chr6.3:6314459-6314478 None:intergenic 45.0%
CCCTCTTGCTTCTCTTGCAA+TGG - chr6.3:6314741-6314760 None:intergenic 50.0%
GATTGTGAGCTTGGAGAGTG+TGG + chr6.3:6315260-6315279 MS.gene20457:CDS 50.0%
GCCATTGCAAGAGAAGCAAG+AGG + chr6.3:6314737-6314756 MS.gene20457:CDS 50.0%
!! AGGAGTTGATGGTGGATGTG+AGG + chr6.3:6315151-6315170 MS.gene20457:CDS 50.0%
GCAAGAGAAGCAAGAGGGGA+TGG + chr6.3:6314743-6314762 MS.gene20457:CDS 55.0%
!! GATGGTGGATGTGAGGCTTG+TGG + chr6.3:6315158-6315177 MS.gene20457:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 6314063 6315346 6314063 ID=MS.gene20457
chr6.3 mRNA 6314063 6315346 6314063 ID=MS.gene20457.t1;Parent=MS.gene20457
chr6.3 exon 6314063 6315346 6314063 ID=MS.gene20457.t1.exon1;Parent=MS.gene20457.t1
chr6.3 CDS 6314063 6315346 6314063 ID=cds.MS.gene20457.t1;Parent=MS.gene20457.t1
Gene Sequence

>MS.gene20457

ATGGGTTGTGTGAGTTCCAAGCAAAAGGGATATTGTCATTGCAACACCCCTTATTGTTCTGTCTCTAGAAGCTACACAATGCATGTACATCATCCACCTCAAAACAAAGGAGATAGTTATCATGTTGTAGCATTGACATCAACAACATTAGGTTCTTTGGAACTTGTATCTCAAAGTCAAGGCAATGGTTTTGCTCCATATTCAACTCTGCTCAAAGCGGACAATACCTCAGAAAAGCAATCCAACGGATTTGAGGTCGGAAAAGGTTTCGTGTTTTCCAATGGTAAGGTTAGTGAGAATTTCAAAGAAGAGGATGAAAATGAGGCGAAGACATGGTCTAATATGATTGAGGAAAAGTTACCAAAATCTATTGCCAAGAAACCAATCACAACACCACCTTGTGAGGACATCATCAACACATGGGAACTAATGGAAGGACTTGAAGATACAAGTCCTTTTCAATCACCAAATCAATTAAGAAGCTTCTCTTTTGATGTTAATGTCAATGGCCATTTTAATCCTTCAAAGCCTAGTTTCATGGAGAATGATGATGGCATTGATTTACACAAACCAAAGTTGGATCATATGATTATTGAGGACGGATCAAATGACTTAAATCTCAAGGTTGAAGTTTTAGATTTTGATGATTGTAAAGTGGTGTCTTCATTCAAGAAGCCATTGCAAGAGAAGCAAGAGGGGATGGATACAAAAAGATTCTTTTTTGAGGAAAAGAATATTAGTGATAATGTTGTGAATTTCAAGTTTGCCCCTTATGAGAAGAAGGAAAAAGTAGTGTTGTATTTCACAAGCCTACGTATGGTGAGAAAAACTTATGAGGATTGTTGCAAAGTTAGGATGATTTTGAAAGGATTAGGCATAAGGGTTGATGAAAGAGATGTGTCAATGCATTTGGGATTCAAAGAAGAGCTTAAAGAGTTACTTAGTGAAGGGAATTATGGTAAAGGAGGATTACCAAAGGTCTTTATTGGAAGGAAATACATTGGTGGAGTTGATGAAATTCAGAAATTACATGATGATAAGAAACTTGAGAAATTGTTTGATTGTTGTGAGAGGATTGATGACATTGAAGGAGTTGATGGTGGATGTGAGGCTTGTGGTGATATAAAGTTTGTTCCTTGTGAAACATGTTATGGAAGTTGTAAAATCTACTATGAAGGTGATTATGAAGAAGATGATGATTGTGAGCTTGGAGAGTGTGGATTCCAAAGGTGTCCTCATTGCAATGAAAATGGTCTAATAAGATGTTCCATGTGTTGTTTCTAG
Protein sequence

>MS.gene20457.t1

MGCVSSKQKGYCHCNTPYCSVSRSYTMHVHHPPQNKGDSYHVVALTSTTLGSLELVSQSQGNGFAPYSTLLKADNTSEKQSNGFEVGKGFVFSNGKVSENFKEEDENEAKTWSNMIEEKLPKSIAKKPITTPPCEDIINTWELMEGLEDTSPFQSPNQLRSFSFDVNVNGHFNPSKPSFMENDDGIDLHKPKLDHMIIEDGSNDLNLKVEVLDFDDCKVVSSFKKPLQEKQEGMDTKRFFFEEKNISDNVVNFKFAPYEKKEKVVLYFTSLRMVRKTYEDCCKVRMILKGLGIRVDERDVSMHLGFKEELKELLSEGNYGKGGLPKVFIGRKYIGGVDEIQKLHDDKKLEKLFDCCERIDDIEGVDGGCEACGDIKFVPCETCYGSCKIYYEGDYEEDDDCELGECGFQRCPHCNENGLIRCSMCCF