AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062638


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062638.t1 MTR_6g066190 85.973 221 24 3 1 218 1 217 7.99e-132 370
MS.gene062638.t1 MTR_6g009650 74.091 220 45 2 1 218 1 210 3.26e-114 325
MS.gene062638.t1 MTR_6g009563 71.233 219 50 3 1 216 1 209 1.94e-107 308
MS.gene062638.t1 MTR_6g066210 66.667 222 64 2 1 218 1 216 7.97e-103 296
MS.gene062638.t1 MTR_6g059730 55.288 208 85 4 1 202 1 206 1.75e-62 194
MS.gene062638.t1 MTR_6g017225 49.362 235 101 5 1 218 1 234 2.55e-62 199
MS.gene062638.t1 MTR_6g059680 53.810 210 89 4 1 202 1 210 8.59e-52 167
MS.gene062638.t1 MTR_1g035770 42.523 214 109 6 1 202 1 212 8.14e-48 157
MS.gene062638.t1 MTR_6g059810 45.833 216 100 8 1 202 1 213 1.35e-43 145
MS.gene062638.t1 MTR_6g065460 45.192 208 102 6 1 200 1 204 3.32e-39 134
MS.gene062638.t1 MTR_6g059650 40.642 187 100 5 22 201 21 203 2.87e-35 124
MS.gene062638.t1 MTR_6g059760 41.232 211 104 11 1 200 1 202 1.62e-29 109
MS.gene062638.t1 MTR_6g059530 40.000 210 105 9 1 202 1 197 5.39e-28 105
MS.gene062638.t1 MTR_6g065460 40.000 205 93 6 1 200 1 180 5.02e-27 102
MS.gene062638.t1 MTR_6g059410 38.164 207 108 8 5 202 1 196 1.07e-25 99.4
MS.gene062638.t1 MTR_6g045403 37.850 214 115 9 1 203 1 207 4.58e-24 95.9
MS.gene062638.t1 MTR_3g463590 37.500 184 98 7 27 200 27 203 6.21e-23 92.4
MS.gene062638.t1 MTR_6g044780 38.053 226 111 13 1 207 1 216 1.45e-22 91.7
MS.gene062638.t1 MTR_6g045433 36.957 230 113 11 1 207 1 221 1.58e-22 91.7
MS.gene062638.t1 MTR_6g044810 37.445 227 111 13 1 207 1 216 1.96e-22 91.3
MS.gene062638.t1 MTR_6g045097 37.885 227 110 14 1 207 1 216 1.34e-21 89.4
MS.gene062638.t1 MTR_1g057110 31.731 208 116 10 1 202 1 188 9.09e-18 78.2
MS.gene062638.t1 MTR_2g073010 31.731 208 114 11 4 200 8 198 2.53e-11 60.8
MS.gene062638.t1 MTR_6g065570 43.662 71 33 1 4 74 9 72 3.92e-11 58.2
MS.gene062638.t1 MTR_6g078070 30.198 202 129 8 7 200 6 203 9.12e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062638.t1 AT1G73325 29.545 220 125 11 7 208 4 211 3.94e-11 60.8
MS.gene062638.t1 AT1G17860 30.814 172 105 7 6 171 3 166 4.70e-11 60.1

Find 50 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAAAACCCATACTAAAT+AGG 0.276635 6.4:-46813264 None:intergenic
CCAAGTATGGTGAATTTAAC+TGG 0.279016 6.4:-46812856 None:intergenic
TATCCTGGTGTAGAATTAAT+TGG 0.295026 6.4:+46813024 MS.gene062638:CDS
CCAGTTAAATTCACCATACT+TGG 0.298543 6.4:+46812856 MS.gene062638:CDS
ACTAGTGATGTTTCCAAGTA+TGG 0.319310 6.4:-46812869 None:intergenic
TGAAACATGGATCTGGATAT+AGG 0.333570 6.4:+46813064 MS.gene062638:CDS
GGACTACAAAAGTTCAATTC+TGG 0.335250 6.4:+46813129 MS.gene062638:CDS
TTACCATGTTTGTCAAATAC+TGG 0.337755 6.4:-46812697 None:intergenic
CTACAAAACAATGCTATAAC+TGG 0.349490 6.4:+46812829 MS.gene062638:CDS
GGAAAGGGTGAGGGATAAAA+CGG 0.381013 6.4:-46812623 None:intergenic
GAAACATGGATCTGGATATA+GGG 0.381905 6.4:+46813065 MS.gene062638:CDS
CTGCTAGGGTTATCACTTGC+TGG 0.383052 6.4:-46812754 None:intergenic
GATTGGTGATGAAAGCAAAA+AGG 0.386990 6.4:-46812651 None:intergenic
GAGGGATAAAACGGGTTTCA+TGG 0.388199 6.4:-46812614 None:intergenic
AAAATGTAGTATTGGCTACT+AGG 0.393835 6.4:-46812730 None:intergenic
TTTGAAGATTCAGCACAATC+TGG 0.404925 6.4:-46812937 None:intergenic
TATTGGCTACTAGGGTTAAG+AGG 0.408482 6.4:-46812721 None:intergenic
TCTACACCAGGATAGTTTCC+AGG 0.412867 6.4:-46813015 None:intergenic
TTAATCTTGAAACATGGATC+TGG 0.434400 6.4:+46813057 MS.gene062638:CDS
AGGGTTAGTCCTCCACTGCT+AGG 0.442500 6.4:-46812769 None:intergenic
ATTGTGCTGAATCTTCAAAA+TGG 0.450225 6.4:+46812941 MS.gene062638:CDS
CAACTAAGTTGTGTTGGTAT+TGG 0.455890 6.4:+46812988 MS.gene062638:CDS
CTAAGTTGTGTTGGTATTGG+TGG 0.456720 6.4:+46812991 MS.gene062638:CDS
CTGAATCACCGACTTTGTCT+AGG 0.458530 6.4:-46812789 None:intergenic
AGCGTTGCTTGGTGAGAGAT+TGG 0.473756 6.4:-46812668 None:intergenic
TGTGTTGGTATTGGTGGTCC+TGG 0.481842 6.4:+46812997 MS.gene062638:CDS
GGTGGTCCTGGAAACTATCC+TGG 0.488659 6.4:+46813009 MS.gene062638:CDS
TTGCTGGTAAAATGTAGTAT+TGG 0.501308 6.4:-46812738 None:intergenic
CCACCAATTAATTCTACACC+AGG 0.511123 6.4:-46813027 None:intergenic
AAAGGAAGAAGGAAAGGGTG+AGG 0.524873 6.4:-46812633 None:intergenic
CATGGATCTGGATATAGGGT+TGG 0.534082 6.4:+46813069 MS.gene062638:CDS
GGGTTAGTCCTCCACTGCTA+GGG 0.534912 6.4:-46812768 None:intergenic
ACTGGAGATTGTGCTTATGT+TGG 0.540839 6.4:+46813108 MS.gene062638:CDS
GAAAGGGTGAGGGATAAAAC+GGG 0.542290 6.4:-46812622 None:intergenic
ATCACTAGTAACATCGTGAC+AGG 0.548264 6.4:+46812883 MS.gene062638:CDS
ATTAATACTATGTCTAATGA+AGG 0.548963 6.4:+46813222 MS.gene062638:CDS
ATTGGCTACTAGGGTTAAGA+GGG 0.549466 6.4:-46812720 None:intergenic
TGAATCACCGACTTTGTCTA+GGG 0.549511 6.4:-46812788 None:intergenic
AAATGTAGTATTGGCTACTA+GGG 0.551896 6.4:-46812729 None:intergenic
AAGGAAGAAGGAAAGGGTGA+GGG 0.562566 6.4:-46812632 None:intergenic
CAAAAGTTCAATTCTGGTGA+AGG 0.564140 6.4:+46813135 MS.gene062638:CDS
CAACCAGTATTTGACAAACA+TGG 0.565333 6.4:+46812694 MS.gene062638:CDS
AATACTCAACTAAGTTGTGT+TGG 0.566729 6.4:+46812982 MS.gene062638:CDS
AAGTTCAATTCTGGTGAAGG+TGG 0.567292 6.4:+46813138 MS.gene062638:CDS
ACTGGTTGAACAGCGTTGCT+TGG 0.579741 6.4:-46812679 None:intergenic
CCTGGTGTAGAATTAATTGG+TGG 0.590080 6.4:+46813027 MS.gene062638:CDS
AGTGAACTCGATCTCAAGAT+CGG 0.599342 6.4:-46812908 None:intergenic
AGTGATAACCCTAGCAGTGG+AGG 0.604496 6.4:+46812760 MS.gene062638:CDS
GCAAGTGATAACCCTAGCAG+TGG 0.634880 6.4:+46812757 MS.gene062638:CDS
GGACTAACCCTAGACAAAGT+CGG 0.647043 6.4:+46812781 MS.gene062638:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTAATCTTGAAACA+TGG + chr6.4:46813051-46813070 MS.gene062638:CDS 15.0%
!! ATTAATACTATGTCTAATGA+AGG + chr6.4:46813222-46813241 MS.gene062638:CDS 20.0%
AATACTCAACTAAGTTGTGT+TGG + chr6.4:46812982-46813001 MS.gene062638:CDS 30.0%
ATTGTGCTGAATCTTCAAAA+TGG + chr6.4:46812941-46812960 MS.gene062638:CDS 30.0%
CTACAAAACAATGCTATAAC+TGG + chr6.4:46812829-46812848 MS.gene062638:CDS 30.0%
TATCCTGGTGTAGAATTAAT+TGG + chr6.4:46813024-46813043 MS.gene062638:CDS 30.0%
TTAATCTTGAAACATGGATC+TGG + chr6.4:46813057-46813076 MS.gene062638:CDS 30.0%
TTACCATGTTTGTCAAATAC+TGG - chr6.4:46812700-46812719 None:intergenic 30.0%
TTGCTGGTAAAATGTAGTAT+TGG - chr6.4:46812741-46812760 None:intergenic 30.0%
! ATATAGGGTTGGATTTTGTT+TGG + chr6.4:46813080-46813099 MS.gene062638:CDS 30.0%
!! AAAATGTAGTATTGGCTACT+AGG - chr6.4:46812733-46812752 None:intergenic 30.0%
!! AAATGTAGTATTGGCTACTA+GGG - chr6.4:46812732-46812751 None:intergenic 30.0%
!! CAGTTATAGCATTGTTTTGT+AGG - chr6.4:46812831-46812850 None:intergenic 30.0%
AAGCAAAAAGGAAGAAGGAA+AGG - chr6.4:46812642-46812661 None:intergenic 35.0%
ACTAGTGATGTTTCCAAGTA+TGG - chr6.4:46812872-46812891 None:intergenic 35.0%
AGCAAAAAGGAAGAAGGAAA+GGG - chr6.4:46812641-46812660 None:intergenic 35.0%
CAAAAGTTCAATTCTGGTGA+AGG + chr6.4:46813135-46813154 MS.gene062638:CDS 35.0%
CAACCAGTATTTGACAAACA+TGG + chr6.4:46812694-46812713 MS.gene062638:CDS 35.0%
CCAAGTATGGTGAATTTAAC+TGG - chr6.4:46812859-46812878 None:intergenic 35.0%
CCAGTTAAATTCACCATACT+TGG + chr6.4:46812856-46812875 MS.gene062638:CDS 35.0%
GAAACATGGATCTGGATATA+GGG + chr6.4:46813065-46813084 MS.gene062638:CDS 35.0%
GATGAAAGCAAAAAGGAAGA+AGG - chr6.4:46812647-46812666 None:intergenic 35.0%
GGACTACAAAAGTTCAATTC+TGG + chr6.4:46813129-46813148 MS.gene062638:CDS 35.0%
TGAAACATGGATCTGGATAT+AGG + chr6.4:46813064-46813083 MS.gene062638:CDS 35.0%
TTTGAAGATTCAGCACAATC+TGG - chr6.4:46812940-46812959 None:intergenic 35.0%
! CAACTAAGTTGTGTTGGTAT+TGG + chr6.4:46812988-46813007 MS.gene062638:CDS 35.0%
!! GATTGGTGATGAAAGCAAAA+AGG - chr6.4:46812654-46812673 None:intergenic 35.0%
!! GGATTTTGTTTGGATACAAC+TGG + chr6.4:46813090-46813109 MS.gene062638:CDS 35.0%
!! TTGTTTTGTAGGACAGTTAG+TGG - chr6.4:46812820-46812839 None:intergenic 35.0%
AAGTTCAATTCTGGTGAAGG+TGG + chr6.4:46813138-46813157 MS.gene062638:CDS 40.0%
ACTGGAGATTGTGCTTATGT+TGG + chr6.4:46813108-46813127 MS.gene062638:CDS 40.0%
AGTGAACTCGATCTCAAGAT+CGG - chr6.4:46812911-46812930 None:intergenic 40.0%
ATCACTAGTAACATCGTGAC+AGG + chr6.4:46812883-46812902 MS.gene062638:CDS 40.0%
ATTGGCTACTAGGGTTAAGA+GGG - chr6.4:46812723-46812742 None:intergenic 40.0%
CCACCAATTAATTCTACACC+AGG - chr6.4:46813030-46813049 None:intergenic 40.0%
CCTGGTGTAGAATTAATTGG+TGG + chr6.4:46813027-46813046 MS.gene062638:CDS 40.0%
! CTAAGTTGTGTTGGTATTGG+TGG + chr6.4:46812991-46813010 MS.gene062638:CDS 40.0%
! TATTGGCTACTAGGGTTAAG+AGG - chr6.4:46812724-46812743 None:intergenic 40.0%
!! TGAATCACCGACTTTGTCTA+GGG - chr6.4:46812791-46812810 None:intergenic 40.0%
AAAGGAAGAAGGAAAGGGTG+AGG - chr6.4:46812636-46812655 None:intergenic 45.0%
AAGGAAGAAGGAAAGGGTGA+GGG - chr6.4:46812635-46812654 None:intergenic 45.0%
CATGGATCTGGATATAGGGT+TGG + chr6.4:46813069-46813088 MS.gene062638:CDS 45.0%
GAAAGGGTGAGGGATAAAAC+GGG - chr6.4:46812625-46812644 None:intergenic 45.0%
GGAAAGGGTGAGGGATAAAA+CGG - chr6.4:46812626-46812645 None:intergenic 45.0%
GGACTAACCCTAGACAAAGT+CGG + chr6.4:46812781-46812800 MS.gene062638:CDS 45.0%
TCTACACCAGGATAGTTTCC+AGG - chr6.4:46813018-46813037 None:intergenic 45.0%
!! CTGAATCACCGACTTTGTCT+AGG - chr6.4:46812792-46812811 None:intergenic 45.0%
ACTGGTTGAACAGCGTTGCT+TGG - chr6.4:46812682-46812701 None:intergenic 50.0%
AGTGATAACCCTAGCAGTGG+AGG + chr6.4:46812760-46812779 MS.gene062638:CDS 50.0%
CTGCTAGGGTTATCACTTGC+TGG - chr6.4:46812757-46812776 None:intergenic 50.0%
GCAAGTGATAACCCTAGCAG+TGG + chr6.4:46812757-46812776 MS.gene062638:CDS 50.0%
! AGCGTTGCTTGGTGAGAGAT+TGG - chr6.4:46812671-46812690 None:intergenic 50.0%
!! TGTGTTGGTATTGGTGGTCC+TGG + chr6.4:46812997-46813016 MS.gene062638:CDS 50.0%
GGTGGTCCTGGAAACTATCC+TGG + chr6.4:46813009-46813028 MS.gene062638:CDS 55.0%
!! AGGGTTAGTCCTCCACTGCT+AGG - chr6.4:46812772-46812791 None:intergenic 55.0%
!! GGGTTAGTCCTCCACTGCTA+GGG - chr6.4:46812771-46812790 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 46812616 46813272 46812616 ID=MS.gene062638
chr6.4 mRNA 46812616 46813272 46812616 ID=MS.gene062638.t1;Parent=MS.gene062638
chr6.4 exon 46812616 46813272 46812616 ID=MS.gene062638.t1.exon1;Parent=MS.gene062638.t1
chr6.4 CDS 46812616 46813272 46812616 ID=cds.MS.gene062638.t1;Parent=MS.gene062638.t1
Gene Sequence

>MS.gene062638

ATGAAACCCGTTTTATCCCTCACCCTTTCCTTCTTCCTTTTTGCTTTCATCACCAATCTCTCACCAAGCAACGCTGTTCAACCAGTATTTGACAAACATGGTAACCCTCTTAACCCTAGTAGCCAATACTACATTTTACCAGCAAGTGATAACCCTAGCAGTGGAGGACTAACCCTAGACAAAGTCGGTGATTCAGAGTGTCCACTAACTGTCCTACAAAACAATGCTATAACTGGTTTACCAGTTAAATTCACCATACTTGGAAACATCACTAGTAACATCGTGACAGGTACCGATCTTGAGATCGAGTTCACTAAGAAGCCAGATTGTGCTGAATCTTCAAAATGGTTGATGTTTGTTGATCATAATACTCAACTAAGTTGTGTTGGTATTGGTGGTCCTGGAAACTATCCTGGTGTAGAATTAATTGGTGGAAAATTTTTAATCTTGAAACATGGATCTGGATATAGGGTTGGATTTTGTTTGGATACAACTGGAGATTGTGCTTATGTTGGACTACAAAAGTTCAATTCTGGTGAAGGTGGATCACGTTTGATTTTGACTGTAACTGATGCTTATTCTGTTGTATTTGTTGATGCTGCTTCTATTAATACTATGTCTAATGAAGGTAATGCAAATAGTGCATTGCCTATTTAG

Protein sequence

>MS.gene062638.t1

MKPVLSLTLSFFLFAFITNLSPSNAVQPVFDKHGNPLNPSSQYYILPASDNPSSGGLTLDKVGDSECPLTVLQNNAITGLPVKFTILGNITSNIVTGTDLEIEFTKKPDCAESSKWLMFVDHNTQLSCVGIGGPGNYPGVELIGGKFLILKHGSGYRVGFCLDTTGDCAYVGLQKFNSGEGGSRLILTVTDAYSVVFVDAASINTMSNEGNANSALPI