Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062852.t1 | XP_013464343.1 | 95.4 | 219 | 10 | 0 | 1 | 219 | 1 | 219 | 9.10E-120 | 439.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062852.t1 | P46417 | 80.6 | 216 | 42 | 0 | 1 | 216 | 1 | 216 | 6.7e-103 | 374.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062852.t1 | B7FGX5 | 95.4 | 219 | 10 | 0 | 1 | 219 | 1 | 219 | 6.6e-120 | 439.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene062852 | 0.831493 | 1.62E-55 | -1.69E-46 |
| MS.gene051525 | MS.gene062852 | 0.801506 | 8.72E-49 | -1.69E-46 |
| MS.gene051568 | MS.gene062852 | 0.807553 | 4.78E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene062852 | 0.801942 | 7.10E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene062852 | -0.825401 | 4.78E-54 | -1.69E-46 |
| MS.gene052333 | MS.gene062852 | 0.809727 | 1.64E-50 | -1.69E-46 |
| MS.gene052389 | MS.gene062852 | 0.819379 | 1.20E-52 | -1.69E-46 |
| MS.gene053176 | MS.gene062852 | 0.820573 | 6.39E-53 | -1.69E-46 |
| MS.gene053494 | MS.gene062852 | 0.803865 | 2.84E-49 | -1.69E-46 |
| MS.gene053555 | MS.gene062852 | 0.809754 | 1.62E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene062852 | 0.829115 | 6.17E-55 | -1.69E-46 |
| MS.gene054468 | MS.gene062852 | 0.807819 | 4.20E-50 | -1.69E-46 |
| MS.gene054548 | MS.gene062852 | 0.809975 | 1.45E-50 | -1.69E-46 |
| MS.gene054556 | MS.gene062852 | 0.820017 | 8.57E-53 | -1.69E-46 |
| MS.gene054559 | MS.gene062852 | 0.806721 | 7.17E-50 | -1.69E-46 |
| MS.gene055356 | MS.gene062852 | 0.813837 | 2.09E-51 | -1.69E-46 |
| MS.gene055367 | MS.gene062852 | 0.826272 | 2.97E-54 | -1.69E-46 |
| MS.gene055369 | MS.gene062852 | 0.809458 | 1.87E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene062852 | 0.809863 | 1.53E-50 | -1.69E-46 |
| MS.gene05595 | MS.gene062852 | 0.814907 | 1.21E-51 | -1.69E-46 |
| MS.gene056297 | MS.gene062852 | 0.822674 | 2.09E-53 | -1.69E-46 |
| MS.gene056723 | MS.gene062852 | 0.847121 | 1.41E-59 | -1.69E-46 |
| MS.gene057469 | MS.gene062852 | 0.830278 | 3.21E-55 | -1.69E-46 |
| MS.gene05872 | MS.gene062852 | 0.818693 | 1.72E-52 | -1.69E-46 |
| MS.gene059128 | MS.gene062852 | 0.803703 | 3.07E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene062852 | 0.812096 | 5.03E-51 | -1.69E-46 |
| MS.gene059609 | MS.gene062852 | 0.805314 | 1.42E-49 | -1.69E-46 |
| MS.gene059958 | MS.gene062852 | 0.805604 | 1.23E-49 | -1.69E-46 |
| MS.gene060942 | MS.gene062852 | 0.813828 | 2.10E-51 | -1.69E-46 |
| MS.gene061002 | MS.gene062852 | 0.815718 | 8.01E-52 | -1.69E-46 |
| MS.gene061114 | MS.gene062852 | -0.818497 | 1.90E-52 | -1.69E-46 |
| MS.gene061233 | MS.gene062852 | 0.811745 | 6.00E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062852.t1 | MTR_2g070070 | 95.434 | 219 | 10 | 0 | 1 | 219 | 1 | 219 | 7.19e-148 | 410 |
| MS.gene062852.t1 | MTR_2g070060 | 89.041 | 219 | 24 | 0 | 1 | 219 | 1 | 219 | 5.66e-147 | 408 |
| MS.gene062852.t1 | MTR_2g070110 | 88.584 | 219 | 24 | 1 | 1 | 219 | 1 | 218 | 1.70e-135 | 379 |
| MS.gene062852.t1 | MTR_2g070180 | 65.888 | 214 | 73 | 0 | 1 | 214 | 1 | 214 | 3.73e-110 | 315 |
| MS.gene062852.t1 | MTR_2g070130 | 62.557 | 219 | 82 | 0 | 1 | 219 | 1 | 219 | 2.70e-107 | 308 |
| MS.gene062852.t1 | MTR_2g070150 | 67.647 | 204 | 66 | 0 | 1 | 204 | 1 | 204 | 4.31e-107 | 306 |
| MS.gene062852.t1 | MTR_2g070140 | 65.517 | 203 | 70 | 0 | 17 | 219 | 1 | 203 | 8.63e-105 | 301 |
| MS.gene062852.t1 | MTR_8g056940 | 65.297 | 219 | 76 | 0 | 1 | 219 | 1 | 219 | 1.03e-104 | 301 |
| MS.gene062852.t1 | MTR_2g070210 | 61.538 | 221 | 83 | 1 | 1 | 219 | 1 | 221 | 7.74e-103 | 296 |
| MS.gene062852.t1 | MTR_3g467420 | 60.829 | 217 | 85 | 0 | 3 | 219 | 8 | 224 | 4.00e-101 | 292 |
| MS.gene062852.t1 | MTR_2g070200 | 60.909 | 220 | 84 | 2 | 1 | 218 | 1 | 220 | 1.00e-98 | 286 |
| MS.gene062852.t1 | MTR_3g467430 | 60.369 | 217 | 86 | 0 | 3 | 219 | 8 | 224 | 7.78e-98 | 284 |
| MS.gene062852.t1 | MTR_4g124130 | 61.364 | 220 | 84 | 1 | 1 | 219 | 1 | 220 | 1.68e-97 | 283 |
| MS.gene062852.t1 | MTR_2g070120 | 57.416 | 209 | 89 | 0 | 1 | 209 | 1 | 209 | 5.96e-88 | 259 |
| MS.gene062852.t1 | MTR_5g076900 | 52.968 | 219 | 101 | 2 | 2 | 219 | 4 | 221 | 5.57e-78 | 233 |
| MS.gene062852.t1 | MTR_0186s0030 | 66.667 | 141 | 47 | 0 | 1 | 141 | 1 | 141 | 3.03e-64 | 196 |
| MS.gene062852.t1 | MTR_5g037380 | 40.807 | 223 | 123 | 3 | 3 | 216 | 4 | 226 | 3.17e-50 | 163 |
| MS.gene062852.t1 | MTR_5g040430 | 36.323 | 223 | 132 | 3 | 2 | 217 | 5 | 224 | 1.04e-47 | 156 |
| MS.gene062852.t1 | MTR_8g087425 | 41.429 | 210 | 116 | 5 | 2 | 206 | 3 | 210 | 9.84e-46 | 151 |
| MS.gene062852.t1 | MTR_1g090060 | 41.509 | 212 | 115 | 4 | 4 | 209 | 3 | 211 | 1.98e-45 | 150 |
| MS.gene062852.t1 | MTR_1g090070 | 41.038 | 212 | 117 | 5 | 4 | 209 | 9 | 218 | 6.85e-43 | 144 |
| MS.gene062852.t1 | MTR_4g019780 | 37.500 | 208 | 125 | 4 | 4 | 208 | 8 | 213 | 1.01e-42 | 144 |
| MS.gene062852.t1 | MTR_4g019790 | 41.232 | 211 | 119 | 4 | 4 | 211 | 7 | 215 | 1.06e-42 | 144 |
| MS.gene062852.t1 | MTR_2g072120 | 88.732 | 71 | 8 | 0 | 47 | 117 | 1 | 71 | 2.06e-42 | 138 |
| MS.gene062852.t1 | MTR_8g087410 | 40.094 | 212 | 119 | 6 | 1 | 206 | 2 | 211 | 1.30e-41 | 141 |
| MS.gene062852.t1 | MTR_7g065630 | 36.866 | 217 | 128 | 5 | 4 | 217 | 25 | 235 | 5.64e-41 | 140 |
| MS.gene062852.t1 | MTR_8g061950 | 37.778 | 225 | 127 | 5 | 3 | 218 | 4 | 224 | 8.96e-41 | 139 |
| MS.gene062852.t1 | MTR_7g065660 | 39.614 | 207 | 120 | 4 | 5 | 208 | 8 | 212 | 1.07e-40 | 139 |
| MS.gene062852.t1 | MTR_7g065750 | 40.796 | 201 | 113 | 4 | 4 | 200 | 7 | 205 | 1.12e-40 | 139 |
| MS.gene062852.t1 | MTR_4g059730 | 39.269 | 219 | 122 | 6 | 1 | 212 | 1 | 215 | 2.78e-40 | 137 |
| MS.gene062852.t1 | MTR_7g065720 | 37.799 | 209 | 124 | 4 | 4 | 208 | 7 | 213 | 5.18e-40 | 137 |
| MS.gene062852.t1 | MTR_6g080440 | 37.321 | 209 | 126 | 4 | 3 | 208 | 2 | 208 | 8.50e-40 | 136 |
| MS.gene062852.t1 | MTR_1g090100 | 41.584 | 202 | 113 | 3 | 11 | 208 | 9 | 209 | 9.67e-40 | 136 |
| MS.gene062852.t1 | MTR_1g090090 | 40.670 | 209 | 119 | 4 | 4 | 208 | 3 | 210 | 2.84e-39 | 135 |
| MS.gene062852.t1 | MTR_7g065740 | 37.799 | 209 | 124 | 4 | 4 | 208 | 7 | 213 | 6.20e-39 | 134 |
| MS.gene062852.t1 | MTR_7g065710 | 36.493 | 211 | 125 | 5 | 1 | 208 | 1 | 205 | 7.40e-38 | 131 |
| MS.gene062852.t1 | MTR_3g099757 | 37.500 | 216 | 132 | 3 | 3 | 216 | 4 | 218 | 9.38e-38 | 131 |
| MS.gene062852.t1 | MTR_7g065590 | 36.574 | 216 | 132 | 4 | 4 | 216 | 7 | 220 | 1.82e-37 | 130 |
| MS.gene062852.t1 | MTR_1g090150 | 36.486 | 222 | 125 | 6 | 4 | 216 | 3 | 217 | 1.62e-36 | 128 |
| MS.gene062852.t1 | MTR_7g065230 | 40.000 | 200 | 110 | 5 | 4 | 200 | 7 | 199 | 1.21e-35 | 125 |
| MS.gene062852.t1 | MTR_7g065265 | 37.981 | 208 | 124 | 4 | 4 | 208 | 7 | 212 | 1.22e-35 | 125 |
| MS.gene062852.t1 | MTR_7g065270 | 38.500 | 200 | 118 | 4 | 4 | 200 | 7 | 204 | 1.53e-35 | 125 |
| MS.gene062852.t1 | MTR_7g065680 | 35.484 | 217 | 131 | 5 | 4 | 217 | 7 | 217 | 3.42e-35 | 124 |
| MS.gene062852.t1 | MTR_7g065600 | 38.191 | 199 | 118 | 4 | 5 | 200 | 8 | 204 | 1.99e-34 | 122 |
| MS.gene062852.t1 | MTR_7g065290 | 37.500 | 208 | 125 | 4 | 4 | 208 | 7 | 212 | 5.74e-34 | 121 |
| MS.gene062852.t1 | MTR_7g065700 | 36.842 | 209 | 127 | 5 | 1 | 208 | 4 | 208 | 2.82e-33 | 119 |
| MS.gene062852.t1 | MTR_7g065640 | 36.275 | 204 | 113 | 4 | 5 | 208 | 8 | 194 | 5.28e-33 | 118 |
| MS.gene062852.t1 | MTR_1g115195 | 35.885 | 209 | 124 | 5 | 4 | 205 | 3 | 208 | 6.11e-33 | 118 |
| MS.gene062852.t1 | MTR_7g065260 | 36.585 | 205 | 125 | 4 | 4 | 205 | 14 | 216 | 1.41e-29 | 110 |
| MS.gene062852.t1 | MTR_5g037390 | 42.045 | 88 | 49 | 1 | 28 | 113 | 11 | 98 | 4.22e-14 | 66.2 |
| MS.gene062852.t1 | MTR_4g043640 | 65.000 | 40 | 14 | 0 | 150 | 189 | 46 | 85 | 9.56e-14 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062852.t1 | AT1G17180 | 64.840 | 219 | 77 | 0 | 1 | 219 | 1 | 219 | 8.46e-110 | 314 |
| MS.gene062852.t1 | AT1G78380 | 67.757 | 214 | 69 | 0 | 1 | 214 | 1 | 214 | 3.07e-109 | 313 |
| MS.gene062852.t1 | AT1G78370 | 63.256 | 215 | 79 | 0 | 1 | 215 | 1 | 215 | 1.25e-102 | 296 |
| MS.gene062852.t1 | AT1G78360 | 63.636 | 220 | 79 | 1 | 1 | 219 | 1 | 220 | 1.12e-101 | 293 |
| MS.gene062852.t1 | AT1G17170 | 63.014 | 219 | 80 | 1 | 1 | 219 | 1 | 218 | 2.04e-101 | 293 |
| MS.gene062852.t1 | AT1G78340 | 61.682 | 214 | 82 | 0 | 1 | 214 | 1 | 214 | 1.44e-99 | 288 |
| MS.gene062852.t1 | AT1G78320 | 61.364 | 220 | 84 | 1 | 1 | 219 | 1 | 220 | 3.42e-96 | 280 |
| MS.gene062852.t1 | AT1G17190 | 60.092 | 218 | 86 | 1 | 1 | 217 | 1 | 218 | 6.79e-95 | 276 |
| MS.gene062852.t1 | AT1G53680 | 56.164 | 219 | 94 | 2 | 3 | 219 | 6 | 224 | 3.05e-90 | 265 |
| MS.gene062852.t1 | AT3G43800 | 53.704 | 216 | 96 | 3 | 4 | 215 | 5 | 220 | 5.61e-82 | 244 |
| MS.gene062852.t1 | AT1G78320 | 53.182 | 220 | 75 | 2 | 1 | 219 | 1 | 193 | 3.24e-76 | 228 |
| MS.gene062852.t1 | AT1G10360 | 42.661 | 218 | 116 | 5 | 1 | 209 | 1 | 218 | 6.40e-53 | 170 |
| MS.gene062852.t1 | AT1G59700 | 44.495 | 218 | 106 | 5 | 4 | 209 | 6 | 220 | 1.01e-50 | 164 |
| MS.gene062852.t1 | AT1G59670 | 45.872 | 218 | 103 | 5 | 4 | 209 | 6 | 220 | 4.95e-49 | 160 |
| MS.gene062852.t1 | AT2G29420 | 41.379 | 203 | 116 | 3 | 2 | 202 | 7 | 208 | 1.60e-47 | 156 |
| MS.gene062852.t1 | AT3G09270 | 40.758 | 211 | 122 | 2 | 5 | 212 | 7 | 217 | 1.60e-47 | 156 |
| MS.gene062852.t1 | AT1G10370 | 41.096 | 219 | 115 | 5 | 2 | 209 | 3 | 218 | 3.34e-47 | 155 |
| MS.gene062852.t1 | AT1G74590 | 41.969 | 193 | 106 | 3 | 3 | 189 | 6 | 198 | 9.65e-46 | 152 |
| MS.gene062852.t1 | AT1G69930 | 39.151 | 212 | 122 | 3 | 5 | 209 | 13 | 224 | 3.17e-42 | 143 |
| MS.gene062852.t1 | AT2G29490 | 37.674 | 215 | 130 | 2 | 5 | 216 | 8 | 221 | 6.78e-42 | 142 |
| MS.gene062852.t1 | AT1G27140 | 39.631 | 217 | 121 | 4 | 3 | 209 | 5 | 221 | 3.37e-41 | 140 |
| MS.gene062852.t1 | AT1G27130 | 38.356 | 219 | 120 | 5 | 3 | 209 | 5 | 220 | 7.16e-41 | 139 |
| MS.gene062852.t1 | AT2G29450 | 40.097 | 207 | 111 | 6 | 4 | 202 | 6 | 207 | 1.32e-39 | 135 |
| MS.gene062852.t1 | AT2G29480 | 40.976 | 205 | 113 | 6 | 5 | 204 | 8 | 209 | 1.74e-39 | 135 |
| MS.gene062852.t1 | AT2G29470 | 38.537 | 205 | 119 | 5 | 5 | 204 | 8 | 210 | 2.13e-39 | 135 |
| MS.gene062852.t1 | AT5G62480 | 34.222 | 225 | 136 | 5 | 1 | 214 | 5 | 228 | 2.43e-39 | 135 |
| MS.gene062852.t1 | AT2G29440 | 38.462 | 221 | 122 | 6 | 4 | 216 | 6 | 220 | 2.56e-39 | 135 |
| MS.gene062852.t1 | AT2G29460 | 40.594 | 202 | 112 | 6 | 4 | 200 | 7 | 205 | 4.85e-39 | 134 |
| MS.gene062852.t1 | AT1G69920 | 36.574 | 216 | 124 | 5 | 5 | 209 | 35 | 248 | 8.78e-38 | 132 |
| MS.gene062852.t1 | AT5G62480 | 31.694 | 183 | 90 | 4 | 1 | 175 | 5 | 160 | 1.28e-22 | 91.7 |
| MS.gene062852.t1 | AT5G62480 | 31.694 | 183 | 91 | 4 | 1 | 175 | 5 | 161 | 1.60e-22 | 91.3 |
Find 44 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAGCTCAAGCTAGATTT+TGG | 0.158333 | 2.4:-25199588 | MS.gene062852:CDS |
| GGTTGATTATATTGATAAAA+AGG | 0.180064 | 2.4:-25199566 | MS.gene062852:intron |
| AGCATAAAACCAGCTGTAAA+AGG | 0.226180 | 2.4:+25198356 | None:intergenic |
| ATTCCAGTTCTTATTCATAA+TGG | 0.270694 | 2.4:-25199700 | MS.gene062852:CDS |
| CATGAAGAAAACACTTGGTT+TGG | 0.291507 | 2.4:-25198199 | MS.gene062852:CDS |
| ATAATTGCAGATTCACAAAT+AGG | 0.302069 | 2.4:+25199673 | None:intergenic |
| TGCAGTATATAGATGAAGTT+TGG | 0.305831 | 2.4:-25199648 | MS.gene062852:CDS |
| GACATATGGTAACTTTAAAA+TGG | 0.308392 | 2.4:-25198328 | MS.gene062852:CDS |
| AAGACACTAGAGGAAACTTT+AGG | 0.341946 | 2.4:-25198435 | MS.gene062852:CDS |
| GCATGTTTGGTATGAGGGTT+AGG | 0.343737 | 2.4:-25199822 | MS.gene062852:CDS |
| AAAGTGGCACCACCGTAAAA+AGG | 0.374250 | 2.4:+25198405 | None:intergenic |
| GATACATGGCCTAGCATGTT+TGG | 0.374420 | 2.4:-25199835 | MS.gene062852:CDS |
| GGCCTAGCATGTTTGGTATG+AGG | 0.389766 | 2.4:-25199828 | MS.gene062852:CDS |
| CTAGCTTGAGCTCTCTCATA+AGG | 0.392919 | 2.4:+25199595 | None:intergenic |
| TTTCCATTATGAATAAGAAC+TGG | 0.412827 | 2.4:+25199697 | None:intergenic |
| TCATACACCTTCATTTGATC+AGG | 0.418180 | 2.4:+25198234 | None:intergenic |
| AGGAAAATGTGGCTTTCAAA+AGG | 0.428209 | 2.4:-25198501 | MS.gene062852:CDS |
| GAATCCAGGTGTATGATACT+TGG | 0.433783 | 2.4:-25198524 | MS.gene062852:intron |
| GCATAAAACCAGCTGTAAAA+GGG | 0.445528 | 2.4:+25198357 | None:intergenic |
| GCCTAGCATGTTTGGTATGA+GGG | 0.468751 | 2.4:-25199827 | MS.gene062852:CDS |
| TTAGCCCATGCCATCAGCTT+AGG | 0.469086 | 2.4:+25198294 | None:intergenic |
| GGCATGGGCTAAGAGATGTT+TGG | 0.494167 | 2.4:-25198283 | MS.gene062852:CDS |
| AACTCTTCCTGATCAAATGA+AGG | 0.495473 | 2.4:-25198241 | MS.gene062852:CDS |
| GGAAAATGTGGCTTTCAAAA+GGG | 0.511061 | 2.4:-25198500 | MS.gene062852:CDS |
| TGGTAACTTTAAAATGGAAG+TGG | 0.524339 | 2.4:-25198322 | MS.gene062852:CDS |
| TAGCTTGAGCTCTCTCATAA+GGG | 0.529940 | 2.4:+25199596 | None:intergenic |
| GGATTCATCTGAAGAAGTAA+AGG | 0.531810 | 2.4:+25199736 | None:intergenic |
| GTGTCCTAAGCTGATGGCAT+GGG | 0.546020 | 2.4:-25198298 | MS.gene062852:CDS |
| AATATCAAGAAAGCCAAAAG+TGG | 0.548628 | 2.4:+25198389 | None:intergenic |
| AGTGTCCTAAGCTGATGGCA+TGG | 0.556692 | 2.4:-25198299 | MS.gene062852:CDS |
| TCCAGGTGTATGATACTTGG+AGG | 0.572955 | 2.4:-25198521 | MS.gene062852:intron |
| AAAGGGGAAGAGCATGAGGA+AGG | 0.578159 | 2.4:-25198483 | MS.gene062852:CDS |
| AAGGGGAAGAGCATGAGGAA+GGG | 0.597445 | 2.4:-25198482 | MS.gene062852:CDS |
| CATAAAACCAGCTGTAAAAG+GGG | 0.600460 | 2.4:+25198358 | None:intergenic |
| GAAAATGTGGCTTTCAAAAG+GGG | 0.601421 | 2.4:-25198499 | MS.gene062852:CDS |
| GCTCTCTCATAAGGGTGAGA+AGG | 0.602075 | 2.4:+25199604 | None:intergenic |
| CTCAATCTTTAAGACACTAG+AGG | 0.604860 | 2.4:-25198445 | MS.gene062852:CDS |
| TACAATCATGGCAAACGAGG+TGG | 0.606147 | 2.4:-25199866 | None:intergenic |
| TTCAAAAGGGGAAGAGCATG+AGG | 0.609682 | 2.4:-25198487 | MS.gene062852:CDS |
| GTGAGCATGAAGAAAACACT+TGG | 0.623270 | 2.4:-25198204 | MS.gene062852:CDS |
| ATGATACTTGGAGGAAAATG+TGG | 0.639144 | 2.4:-25198512 | MS.gene062852:CDS |
| ACCCTCATACCAAACATGCT+AGG | 0.644205 | 2.4:+25199826 | None:intergenic |
| TTCTACAATCATGGCAAACG+AGG | 0.652977 | 2.4:-25199869 | None:intergenic |
| AGTGGAGTGTCCTAAGCTGA+TGG | 0.664603 | 2.4:-25198304 | MS.gene062852:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATGATATAAATATAGT+AGG | - | chr2.4:25199147-25199166 | MS.gene062852:intron | 10.0% |
| !!! | CATATTTAAGTATTTTTTAT+TGG | - | chr2.4:25199171-25199190 | MS.gene062852:intron | 10.0% |
| !! | CAACTTAATGAAATAATTTA+TGG | - | chr2.4:25198760-25198779 | MS.gene062852:intron | 15.0% |
| !! | TTTAATAAGATTCAAGTAAT+AGG | + | chr2.4:25198683-25198702 | None:intergenic | 15.0% |
| !!! | ATTTTTATTCTTAATTTTGG+TGG | - | chr2.4:25198536-25198555 | MS.gene062852:intron | 15.0% |
| !!! | TTTATTACTTTTTTAAACGT+GGG | + | chr2.4:25199032-25199051 | None:intergenic | 15.0% |
| !!! | TTTTATTACTTTTTTAAACG+TGG | + | chr2.4:25199033-25199052 | None:intergenic | 15.0% |
| !!! | TTTTTATTCTTAATTTTGGT+GGG | - | chr2.4:25198537-25198556 | MS.gene062852:intron | 15.0% |
| !! | AATAAAAGGAAAACAAATCA+AGG | + | chr2.4:25198519-25198538 | None:intergenic | 20.0% |
| !! | GGTTGATTATATTGATAAAA+AGG | - | chr2.4:25198487-25198506 | MS.gene062852:CDS | 20.0% |
| !! | TATGATCAAGAATGATTATT+TGG | + | chr2.4:25199474-25199493 | None:intergenic | 20.0% |
| !! | TGGATAAACAATTTACTTAA+AGG | + | chr2.4:25199454-25199473 | None:intergenic | 20.0% |
| ! | AATTGTAAATCCTCTTATGA+CGG | - | chr2.4:25198825-25198844 | MS.gene062852:intron | 25.0% |
| ! | ATAATTGCAGATTCACAAAT+AGG | + | chr2.4:25198383-25198402 | None:intergenic | 25.0% |
| ! | ATTCCAGTTCTTATTCATAA+TGG | - | chr2.4:25198353-25198372 | MS.gene062852:CDS | 25.0% |
| ! | CAAATGGTAATACACTAATA+AGG | + | chr2.4:25199200-25199219 | None:intergenic | 25.0% |
| ! | GTAATAAAATGTGTACTACA+TGG | - | chr2.4:25199085-25199104 | MS.gene062852:intron | 25.0% |
| ! | GTAATAAAATGTGTACTACT+TGG | - | chr2.4:25199044-25199063 | MS.gene062852:intron | 25.0% |
| ! | TAATTGTTCATGATTAACAC+TGG | - | chr2.4:25198607-25198626 | MS.gene062852:intron | 25.0% |
| ! | TTACGTCTTATAAAACAAGA+CGG | - | chr2.4:25198799-25198818 | MS.gene062852:intron | 25.0% |
| ! | TTTCCATTATGAATAAGAAC+TGG | + | chr2.4:25198359-25198378 | None:intergenic | 25.0% |
| !! | ACTGGAATTTTCTTATGAAT+TGG | + | chr2.4:25198341-25198360 | None:intergenic | 25.0% |
| !! | GACATATGGTAACTTTAAAA+TGG | - | chr2.4:25199725-25199744 | MS.gene062852:CDS | 25.0% |
| !! | TATTACTCCTTTTTACAAGT+GGG | + | chr2.4:25199112-25199131 | None:intergenic | 25.0% |
| !! | TTTTATGCTTATGAGACATA+TGG | - | chr2.4:25199711-25199730 | MS.gene062852:CDS | 25.0% |
| !!! | ATTTTTTCTTCCTTTAATGG+AGG | + | chr2.4:25198946-25198965 | None:intergenic | 25.0% |
| !!! | CTCATTTTTTCTTCCTTTAA+TGG | + | chr2.4:25198949-25198968 | None:intergenic | 25.0% |
| !!! | TTTTTTCTTCCTTTAATGGA+GGG | + | chr2.4:25198945-25198964 | None:intergenic | 25.0% |
| AAAGCACAAAAGTTACCAAA+AGG | + | chr2.4:25198653-25198672 | None:intergenic | 30.0% | |
| AATATCAAGAAAGCCAAAAG+TGG | + | chr2.4:25199667-25199686 | None:intergenic | 30.0% | |
| CATTTAAAGAGTCCTTGTTA+GGG | - | chr2.4:25199293-25199312 | MS.gene062852:intron | 30.0% | |
| TAAGTACTCAATGACTCAAA+TGG | + | chr2.4:25199216-25199235 | None:intergenic | 30.0% | |
| TATTACTCCTTTATGCAAGT+GGG | + | chr2.4:25199071-25199090 | None:intergenic | 30.0% | |
| TGCAGTATATAGATGAAGTT+TGG | - | chr2.4:25198405-25198424 | MS.gene062852:CDS | 30.0% | |
| TTATTACTCCTTTATGCAAG+TGG | + | chr2.4:25199072-25199091 | None:intergenic | 30.0% | |
| ! | CTATTACTCCTTTTTACAAG+TGG | + | chr2.4:25199113-25199132 | None:intergenic | 30.0% |
| ! | TTTGTGCTAGATTTACCTTT+TGG | - | chr2.4:25198635-25198654 | MS.gene062852:intron | 30.0% |
| !! | TGGTAACTTTAAAATGGAAG+TGG | - | chr2.4:25199731-25199750 | MS.gene062852:CDS | 30.0% |
| !!! | ACTTTTGGCTTTCTTGATAT+TGG | - | chr2.4:25199666-25199685 | MS.gene062852:CDS | 30.0% |
| !!! | TCATTGTTGATTTTGAATCC+AGG | - | chr2.4:25199515-25199534 | MS.gene062852:intron | 30.0% |
| AAATAAGTGATGTGTCACGT+GGG | - | chr2.4:25199267-25199286 | MS.gene062852:intron | 35.0% | |
| AACTCTTCCTGATCAAATGA+AGG | - | chr2.4:25199812-25199831 | MS.gene062852:CDS | 35.0% | |
| AAGACACTAGAGGAAACTTT+AGG | - | chr2.4:25199618-25199637 | MS.gene062852:CDS | 35.0% | |
| AAGGAACGGACAACTATATA+AGG | - | chr2.4:25198977-25198996 | MS.gene062852:intron | 35.0% | |
| AGCATAAAACCAGCTGTAAA+AGG | + | chr2.4:25199700-25199719 | None:intergenic | 35.0% | |
| AGGAAAATGTGGCTTTCAAA+AGG | - | chr2.4:25199552-25199571 | MS.gene062852:intron | 35.0% | |
| ATGATACTTGGAGGAAAATG+TGG | - | chr2.4:25199541-25199560 | MS.gene062852:intron | 35.0% | |
| CATAAAACCAGCTGTAAAAG+GGG | + | chr2.4:25199698-25199717 | None:intergenic | 35.0% | |
| CATGAAGAAAACACTTGGTT+TGG | - | chr2.4:25199854-25199873 | MS.gene062852:CDS | 35.0% | |
| CCATTTAAAGAGTCCTTGTT+AGG | - | chr2.4:25199292-25199311 | MS.gene062852:intron | 35.0% | |
| CCTAACAAGGACTCTTTAAA+TGG | + | chr2.4:25199295-25199314 | None:intergenic | 35.0% | |
| GAAAAAATGAGTCTCCGTTA+AGG | - | chr2.4:25198958-25198977 | MS.gene062852:intron | 35.0% | |
| GAAAATGTGGCTTTCAAAAG+GGG | - | chr2.4:25199554-25199573 | MS.gene062852:intron | 35.0% | |
| GCATAAAACCAGCTGTAAAA+GGG | + | chr2.4:25199699-25199718 | None:intergenic | 35.0% | |
| GGAAAATGTGGCTTTCAAAA+GGG | - | chr2.4:25199553-25199572 | MS.gene062852:intron | 35.0% | |
| GGATTCATCTGAAGAAGTAA+AGG | + | chr2.4:25198320-25198339 | None:intergenic | 35.0% | |
| TAAATAAGTGATGTGTCACG+TGG | - | chr2.4:25199266-25199285 | MS.gene062852:intron | 35.0% | |
| TATAGTTGTCCGTTCCTTAA+CGG | + | chr2.4:25198975-25198994 | None:intergenic | 35.0% | |
| TCATACACCTTCATTTGATC+AGG | + | chr2.4:25199822-25199841 | None:intergenic | 35.0% | |
| ! | TTAGGTGACAAGCCTTTTTA+CGG | - | chr2.4:25199636-25199655 | MS.gene062852:CDS | 35.0% |
| !! | CTCAATCTTTAAGACACTAG+AGG | - | chr2.4:25199608-25199627 | MS.gene062852:CDS | 35.0% |
| !!! | ATTTTCAAGAGCATCCACAA+TGG | + | chr2.4:25199324-25199343 | None:intergenic | 35.0% |
| AATAAGTGATGTGTCACGTG+GGG | - | chr2.4:25199268-25199287 | MS.gene062852:intron | 40.0% | |
| AATGAGTCTCCGTTAAGGAA+CGG | - | chr2.4:25198963-25198982 | MS.gene062852:intron | 40.0% | |
| ACAATGGTAGTTCCCTAACA+AGG | + | chr2.4:25199308-25199327 | None:intergenic | 40.0% | |
| ACATGGTCCCACTTGTAAAA+AGG | - | chr2.4:25199102-25199121 | MS.gene062852:intron | 40.0% | |
| CTTTAATGGAGGGTCCTTAA+CGG | + | chr2.4:25198935-25198954 | None:intergenic | 40.0% | |
| GAATCCAGGTGTATGATACT+TGG | - | chr2.4:25199529-25199548 | MS.gene062852:intron | 40.0% | |
| GTGAGCATGAAGAAAACACT+TGG | - | chr2.4:25199849-25199868 | MS.gene062852:CDS | 40.0% | |
| TAGAGTTCAGCATCTCCAAT+GGG | - | chr2.4:25198882-25198901 | MS.gene062852:intron | 40.0% | |
| TAGCTTGAGCTCTCTCATAA+GGG | + | chr2.4:25198460-25198479 | None:intergenic | 40.0% | |
| TTAGAGTTCAGCATCTCCAA+TGG | - | chr2.4:25198881-25198900 | MS.gene062852:intron | 40.0% | |
| TTGTTAGGGAACTACCATTG+TGG | - | chr2.4:25199307-25199326 | MS.gene062852:intron | 40.0% | |
| TTTAATGGAGGGTCCTTAAC+GGG | + | chr2.4:25198934-25198953 | None:intergenic | 40.0% | |
| ! | AGAGAGCTCAAGCTAGATTT+TGG | - | chr2.4:25198465-25198484 | MS.gene062852:CDS | 40.0% |
| ! | GAGAGCTCAAGCTAGATTTT+GGG | - | chr2.4:25198466-25198485 | MS.gene062852:CDS | 40.0% |
| !!! | AGGTGGTTTTGTTGGATACA+TGG | - | chr2.4:25198204-25198223 | MS.gene062852:CDS | 40.0% |
| AAAGTGGCACCACCGTAAAA+AGG | + | chr2.4:25199651-25199670 | None:intergenic | 45.0% | |
| ACCCTCATACCAAACATGCT+AGG | + | chr2.4:25198230-25198249 | None:intergenic | 45.0% | |
| ACTTGGTCCCACTTGCATAA+AGG | - | chr2.4:25199061-25199080 | MS.gene062852:intron | 45.0% | |
| AGAGTTCAGCATCTCCAATG+GGG | - | chr2.4:25198883-25198902 | MS.gene062852:intron | 45.0% | |
| CGTTAAGGACCCTCCATTAA+AGG | - | chr2.4:25198933-25198952 | MS.gene062852:intron | 45.0% | |
| CTAGCTTGAGCTCTCTCATA+AGG | + | chr2.4:25198461-25198480 | None:intergenic | 45.0% | |
| GATACATGGCCTAGCATGTT+TGG | - | chr2.4:25198218-25198237 | MS.gene062852:CDS | 45.0% | |
| GCATGTTTGGTATGAGGGTT+AGG | - | chr2.4:25198231-25198250 | MS.gene062852:CDS | 45.0% | |
| GCCTAGCATGTTTGGTATGA+GGG | - | chr2.4:25198226-25198245 | MS.gene062852:CDS | 45.0% | |
| GGAGTGGTCCTTGTTAAGTA+AGG | + | chr2.4:25198912-25198931 | None:intergenic | 45.0% | |
| TCCAGGTGTATGATACTTGG+AGG | - | chr2.4:25199532-25199551 | MS.gene062852:intron | 45.0% | |
| TCCTCCAAGTATCATACACC+TGG | + | chr2.4:25199536-25199555 | None:intergenic | 45.0% | |
| TCTCTCTCCTGGGTAACTAA+CGG | - | chr2.4:25199004-25199023 | MS.gene062852:intron | 45.0% | |
| TTAATGGAGGGTCCTTAACG+GGG | + | chr2.4:25198933-25198952 | None:intergenic | 45.0% | |
| TTAGTTACCCAGGAGAGAGA+GGG | + | chr2.4:25199004-25199023 | None:intergenic | 45.0% | |
| ! | TTCAAAAGGGGAAGAGCATG+AGG | - | chr2.4:25199566-25199585 | MS.gene062852:intron | 45.0% |
| !!! | TTTTACGGTGGTGCCACTTT+TGG | - | chr2.4:25199651-25199670 | MS.gene062852:CDS | 45.0% |
| !! | AAATTAAGAATAAAAATAAA+AGG | + | chr2.4:25198533-25198552 | None:intergenic | 5.0% |
| !!! | TTTATTTTTATTCTTAATTT+TGG | - | chr2.4:25198533-25198552 | MS.gene062852:intron | 5.0% |
| AGTGTCCTAAGCTGATGGCA+TGG | - | chr2.4:25199754-25199773 | MS.gene062852:CDS | 50.0% | |
| AGTTCAGCATCTCCAATGGG+GGG | - | chr2.4:25198885-25198904 | MS.gene062852:intron | 50.0% | |
| ATAAGGACCCTCTCTCTCCT+GGG | - | chr2.4:25198994-25199013 | MS.gene062852:intron | 50.0% | |
| CGATAGTCGTCCGTCATAAG+AGG | + | chr2.4:25198838-25198857 | None:intergenic | 50.0% | |
| CTCTCTCCTGGGTAACTAAC+GGG | - | chr2.4:25199005-25199024 | MS.gene062852:intron | 50.0% | |
| GAGTTCAGCATCTCCAATGG+GGG | - | chr2.4:25198884-25198903 | MS.gene062852:intron | 50.0% | |
| GCTCTCTCATAAGGGTGAGA+AGG | + | chr2.4:25198452-25198471 | None:intergenic | 50.0% | |
| GGCCTAGCATGTTTGGTATG+AGG | - | chr2.4:25198225-25198244 | MS.gene062852:CDS | 50.0% | |
| GTGTCCTAAGCTGATGGCAT+GGG | - | chr2.4:25199755-25199774 | MS.gene062852:CDS | 50.0% | |
| GTTAAGTAAGGACCCCCCAT+TGG | + | chr2.4:25198900-25198919 | None:intergenic | 50.0% | |
| GTTAGTTACCCAGGAGAGAG+AGG | + | chr2.4:25199005-25199024 | None:intergenic | 50.0% | |
| TATAAGGACCCTCTCTCTCC+TGG | - | chr2.4:25198993-25199012 | MS.gene062852:intron | 50.0% | |
| TGGGGGGTCCTTACTTAACA+AGG | - | chr2.4:25198901-25198920 | MS.gene062852:intron | 50.0% | |
| TTAGCCCATGCCATCAGCTT+AGG | + | chr2.4:25199762-25199781 | None:intergenic | 50.0% | |
| ! | AAAGGGGAAGAGCATGAGGA+AGG | - | chr2.4:25199570-25199589 | MS.gene062852:CDS | 50.0% |
| ! | AAGGGGAAGAGCATGAGGAA+GGG | - | chr2.4:25199571-25199590 | MS.gene062852:CDS | 50.0% |
| ! | AGTGGAGTGTCCTAAGCTGA+TGG | - | chr2.4:25199749-25199768 | MS.gene062852:CDS | 50.0% |
| ! | GACTGATCCCCTTTTACAGC+TGG | - | chr2.4:25199688-25199707 | MS.gene062852:CDS | 50.0% |
| ! | GGCATGGGCTAAGAGATGTT+TGG | - | chr2.4:25199770-25199789 | MS.gene062852:CDS | 50.0% |
| ! | GGTGACAAGCCTTTTTACGG+TGG | - | chr2.4:25199639-25199658 | MS.gene062852:CDS | 50.0% |
| !!! | GGCAAACGAGGTGGTTTTGT+TGG | - | chr2.4:25198196-25198215 | MS.gene062852:CDS | 50.0% |
| ACAAGGACCACTCCCCGTTA+AGG | - | chr2.4:25198918-25198937 | MS.gene062852:intron | 55.0% | |
| GTGGGACCCGTTAGTTACCC+AGG | + | chr2.4:25199014-25199033 | None:intergenic | 60.0% | |
| GGAGGGTCCTTAACGGGGAG+TGG | + | chr2.4:25198928-25198947 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 25198194 | 25199881 | 25198194 | ID=MS.gene062852 |
| chr2.4 | mRNA | 25198194 | 25199881 | 25198194 | ID=MS.gene062852.t1;Parent=MS.gene062852 |
| chr2.4 | exon | 25199567 | 25199881 | 25199567 | ID=MS.gene062852.t1.exon1;Parent=MS.gene062852.t1 |
| chr2.4 | CDS | 25199567 | 25199881 | 25199567 | ID=cds.MS.gene062852.t1;Parent=MS.gene062852.t1 |
| chr2.4 | exon | 25198194 | 25198538 | 25198194 | ID=MS.gene062852.t1.exon2;Parent=MS.gene062852.t1 |
| chr2.4 | CDS | 25198194 | 25198538 | 25198194 | ID=cds.MS.gene062852.t1;Parent=MS.gene062852.t1 |
| Gene Sequence |
| Protein sequence |
