AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061002


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061002.t1 MTR_4g121940 96.296 216 8 0 1 216 16 231 1.42e-158 437
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061002.t1 AT3G16990 61.395 215 83 0 1 215 6 220 1.17e-99 288

Find 0 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTATATTAATAGAGATG+TGG + 32439:11266-11285 MS.gene061002:intron 20.0%
!! TCAAATAAGTCAATTTAAAC+AGG - 32439:11138-11157 None:intergenic 20.0%
!!! AGTTGTTTTTCCATATAAAA+GGG + 32439:11203-11222 MS.gene061002:intron 20.0%
!!! TCAATTTATTTCAATTTTGC+AGG + 32439:10776-10795 MS.gene061002:intron 20.0%
!!! TTCATAATGAGTTCTAATAA+CGG - 32439:11056-11075 None:intergenic 20.0%
! ACTCATTATGAAGGTTTATT+TGG + 32439:11064-11083 MS.gene061002:intron 25.0%
! ATACATGCTCATAATTATCT+AGG - 32439:11632-11651 None:intergenic 25.0%
! CTCATTATGAAGGTTTATTT+GGG + 32439:11065-11084 MS.gene061002:intron 25.0%
! GTTATTAGAACTCATTATGA+AGG + 32439:11055-11074 MS.gene061002:intron 25.0%
! TACATGCTCATAATTATCTA+GGG - 32439:11631-11650 None:intergenic 25.0%
! TCATTATGAAGGTTTATTTG+GGG + 32439:11066-11085 MS.gene061002:intron 25.0%
!! TAGTTTTTGTTAGGTTTTTG+AGG - 32439:10990-11009 None:intergenic 25.0%
!!! CAGTTGTTTTTCCATATAAA+AGG + 32439:11202-11221 MS.gene061002:intron 25.0%
AACCTAACAAAAACTATTGC+AGG + 32439:10994-11013 MS.gene061002:CDS 30.0%
AATCTCATTCCTTCAAAACA+TGG + 32439:10698-10717 MS.gene061002:CDS 30.0%
ACATGCTCATAATTATCTAG+GGG - 32439:11630-11649 None:intergenic 30.0%
ACTAACAAAGTCTACATTCA+GGG + 32439:11528-11547 MS.gene061002:intron 30.0%
CTCTCAAGGATGAAATATTA+TGG + 32439:10916-10935 MS.gene061002:CDS 30.0%
GATGATGTGGAAGTTATATT+GGG + 32439:10882-10901 MS.gene061002:CDS 30.0%
TTGAACATGAAGTTCATTTC+TGG + 32439:11932-11951 MS.gene061002:CDS 30.0%
TTGCAATTTATCTGCATTCA+GGG + 32439:11557-11576 MS.gene061002:intron 30.0%
TTGCAATTTCTCTTCATTCA+GGG - 32439:11407-11426 None:intergenic 30.0%
TTTGAAGGAATGAGATTGAA+CGG - 32439:10695-10714 None:intergenic 30.0%
TTTGACCTTATCTACTGTAT+AGG + 32439:11153-11172 MS.gene061002:intron 30.0%
TTTGCAATTTATCTGCATTC+AGG + 32439:11556-11575 MS.gene061002:intron 30.0%
TTTGCAATTTCTCTTCATTC+AGG - 32439:11408-11427 None:intergenic 30.0%
! TACCTGCAATAGTTTTTGTT+AGG - 32439:10999-11018 None:intergenic 30.0%
! TGATGATGTGGAAGTTATAT+TGG + 32439:10881-10900 MS.gene061002:CDS 30.0%
!!! TCTAGTTTTTGCTAGTATAC+CGG + 32439:11108-11127 MS.gene061002:intron 30.0%
AAAAACACCACCAGAATTGA+AGG + 32439:11777-11796 MS.gene061002:CDS 35.0%
AAGTTTGATGAGTCAAGATG+TGG + 32439:11672-11691 MS.gene061002:CDS 35.0%
ACTAACGAAGTTTGCATTCA+GGG + 32439:11464-11483 MS.gene061002:intron 35.0%
GACTAACAAAGTCTACATTC+AGG + 32439:11527-11546 MS.gene061002:intron 35.0%
GTTCTCCTATACAGTAGATA+AGG - 32439:11161-11180 None:intergenic 35.0%
TAAGAGAGAAGCCAACAAAT+GGG + 32439:10941-10960 MS.gene061002:CDS 35.0%
TTAAGAGAGAAGCCAACAAA+TGG + 32439:10940-10959 MS.gene061002:CDS 35.0%
TTTGCACACTGTATTGAAGA+AGG + 32439:11751-11770 MS.gene061002:CDS 35.0%
! AATGAGTTCTAATAACGGCA+TGG - 32439:11051-11070 None:intergenic 35.0%
! GTTACACACACCCTTTTATA+TGG - 32439:11216-11235 None:intergenic 35.0%
! TATTTCAATTTTGCAGGCTC+AGG + 32439:10782-10801 MS.gene061002:intron 35.0%
! TGACTATCTTTTGCAGAGAT+TGG - 32439:11846-11865 None:intergenic 35.0%
! TTCTGATGATGAGCTAAAGA+AGG + 32439:11885-11904 MS.gene061002:CDS 35.0%
!! AGTCATTTTAGTCCCTGAAT+GGG + 32439:11361-11380 MS.gene061002:intron 35.0%
!! TAGTCATTTTAGTCCCTGAA+TGG + 32439:11360-11379 MS.gene061002:intron 35.0%
AAGAGAGAAGCCAACAAATG+GGG + 32439:10942-10961 MS.gene061002:CDS 40.0%
AAGGAGACTTGTGAAAGATG+GGG + 32439:11796-11815 MS.gene061002:CDS 40.0%
ATCAGCACACTAGCAACAAA+TGG - 32439:10834-10853 None:intergenic 40.0%
CACGGATGGAGAGAATAAAA+GGG - 32439:10666-10685 None:intergenic 40.0%
GAAGGAGACTTGTGAAAGAT+GGG + 32439:11795-11814 MS.gene061002:CDS 40.0%
GACTAACGAAGTTTGCATTC+AGG + 32439:11463-11482 MS.gene061002:intron 40.0%
GAGTGAAATACCCCATTTGT+TGG - 32439:10955-10974 None:intergenic 40.0%
GATGTGGAAGTTATATTGGG+AGG + 32439:10885-10904 MS.gene061002:CDS 40.0%
GGAAGTTATATTGGGAGGTA+TGG + 32439:10890-10909 MS.gene061002:CDS 40.0%
GTCAATTTAAACAGGCCTAC+CGG - 32439:11130-11149 None:intergenic 40.0%
TCACAAGTCTCCTTCAATTC+TGG - 32439:11790-11809 None:intergenic 40.0%
TCACGGATGGAGAGAATAAA+AGG - 32439:10667-10686 None:intergenic 40.0%
TGAAGGAGACTTGTGAAAGA+TGG + 32439:11794-11813 MS.gene061002:CDS 40.0%
TGAGCATGTATTGTTGTGAC+AGG + 32439:11641-11660 MS.gene061002:intron 40.0%
TTTATTCTCTCCATCCGTGA+TGG + 32439:10667-10686 MS.gene061002:CDS 40.0%
! AGTCACTTTAGTCCCTGAAT+CGG - 32439:11603-11622 None:intergenic 40.0%
! ATCAGATGACAGTGATGATG+TGG + 32439:10869-10888 MS.gene061002:CDS 40.0%
! GTTTTTGCTAGTATACCGGT+AGG + 32439:11112-11131 MS.gene061002:intron 40.0%
! TCAAGATGTGGATTATACCG+TGG + 32439:11684-11703 MS.gene061002:CDS 40.0%
! TGCATTTTGGGCAATTGAAG+TGG + 32439:11714-11733 MS.gene061002:CDS 40.0%
!! ACTTACGAGCCATGTTTTGA+AGG - 32439:10710-10729 None:intergenic 40.0%
!! TGTGCTGATTAAAGCTTGCA+AGG + 32439:10845-10864 MS.gene061002:CDS 40.0%
ACTTTAGTCCCTGAATCGGA+AGG - 32439:11599-11618 None:intergenic 45.0%
AGTCAATCAACGCTTGCAGA+AGG + 32439:11861-11880 MS.gene061002:CDS 45.0%
ATTGCAAAACAGTCCCTGCA+TGG + 32439:11420-11439 MS.gene061002:intron 45.0%
CAAGTCTCCTTCAATTCTGG+TGG - 32439:11787-11806 None:intergenic 45.0%
CCAAAATGCAGTAAGAGCCA+CGG - 32439:11704-11723 None:intergenic 45.0%
CTTTAGTCCCTGAATCGGAA+GGG - 32439:11598-11617 None:intergenic 45.0%
GACGAATCATCTCCCATTCA+GGG - 32439:11376-11395 None:intergenic 45.0%
GTATTGTTGTGACAGGTTGC+TGG + 32439:11648-11667 MS.gene061002:intron 45.0%
GTGAAAGATGGGGCAATGAA+GGG + 32439:11806-11825 MS.gene061002:CDS 45.0%
TCCCTGAAATTGACCATGCA+GGG - 32439:11436-11455 None:intergenic 45.0%
TCCCTGCATGGTCAATTTCA+GGG + 32439:11432-11451 MS.gene061002:intron 45.0%
TGACGAATCATCTCCCATTC+AGG - 32439:11377-11396 None:intergenic 45.0%
TGTGAAAGATGGGGCAATGA+AGG + 32439:11805-11824 MS.gene061002:CDS 45.0%
! CGTGGCTCTTACTGCATTTT+GGG + 32439:11702-11721 MS.gene061002:CDS 45.0%
!! CATTTCTGGAATATGAGCCG+TGG + 32439:11946-11965 MS.gene061002:CDS 45.0%
!! GGAGAGAATAAAAGGGTGTC+TGG - 32439:10659-10678 None:intergenic 45.0%
AGATGGGGCAATGAAGGGTT+TGG + 32439:11811-11830 MS.gene061002:CDS 50.0%
GACGAATCCCTTCCGATTCA+GGG + 32439:11588-11607 MS.gene061002:intron 50.0%
GTCCCTGAAATTGACCATGC+AGG - 32439:11437-11456 None:intergenic 50.0%
GTCCCTGCATGGTCAATTTC+AGG + 32439:11431-11450 MS.gene061002:intron 50.0%
TGACGAATCCCTTCCGATTC+AGG + 32439:11587-11606 MS.gene061002:intron 50.0%
TTGCTGCGTCGTAGATAGGA+CGG - 32439:10636-10655 None:intergenic 50.0%
! CCGTGGCTCTTACTGCATTT+TGG + 32439:11701-11720 MS.gene061002:CDS 50.0%
!! GAGATTGAACGGTGCCATCA+CGG - 32439:10684-10703 None:intergenic 50.0%
CTGGTTGCTGCGTCGTAGAT+AGG - 32439:10640-10659 None:intergenic 55.0%
! GGGAGGTATGGCTTCTCTCA+AGG + 32439:10902-10921 MS.gene061002:CDS 55.0%
!! TTGAACGGTGCCATCACGGA+TGG - 32439:10680-10699 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
32439 gene 10607 11978 10607 ID=MS.gene061002
32439 mRNA 10607 11978 10607 ID=MS.gene061002.t1;Parent=MS.gene061002
32439 exon 10607 10723 10607 ID=MS.gene061002.t1.exon1;Parent=MS.gene061002.t1
32439 CDS 10607 10723 10607 ID=cds.MS.gene061002.t1;Parent=MS.gene061002.t1
32439 exon 10798 11015 10798 ID=MS.gene061002.t1.exon2;Parent=MS.gene061002.t1
32439 CDS 10798 11015 10798 ID=cds.MS.gene061002.t1;Parent=MS.gene061002.t1
32439 exon 11663 11978 11663 ID=MS.gene061002.t1.exon3;Parent=MS.gene061002.t1
32439 CDS 11663 11978 11663 ID=cds.MS.gene061002.t1;Parent=MS.gene061002.t1
Gene Sequence

>MS.gene061002

ATGACAGAAACATGGTTGAGGAAACACCGTCCTATCTACGACGCAGCAACCAGACACCCTTTTATTCTCTCCATCCGTGATGGCACCGTTCAATCTCATTCCTTCAAAACATGGCTCGTAAGTAATTTTCAATACATCTCTCTCTTCTCTTTTTATTAGTATCATTTTTTCAATTTATTTCAATTTTGCAGGCTCAGGATTACTTGTTTGTTAGAGAGTTTGTTCCATTTGTTGCTAGTGTGCTGATTAAAGCTTGCAAGGAATCAGATGACAGTGATGATGTGGAAGTTATATTGGGAGGTATGGCTTCTCTCAAGGATGAAATATTATGGTTTAAGAGAGAAGCCAACAAATGGGGTATTTCACTCTCAGACGTTGTTCCTCAAAAACCTAACAAAAACTATTGCAGGTATGTTTATTAGTCATCGATTTTTGTTCTAACCATGCCGTTATTAGAACTCATTATGAAGGTTTATTTGGGGTTTTAAATCATCTGTTGTTTCTAGTTTTTGCTAGTATACCGGTAGGCCTGTTTAAATTGACTTATTTGACCTTATCTACTGTATAGGAGAACTTATGAAGACATGTTCAATTTCAGTTGTTTTTCCATATAAAAGGGTGTGTGTAACATTTCTTATAACTATAATATAATTTCGATACAATTATATTAATAGAGATGTGGTAGTAATTTTTTTACATTGACTGCAAACAAGAAGTCTTAATTTTATTTTAAGTGTTTAAAGTAGATAGAATTAGTCATTTTAGTCCCTGAATGGGAGATGATTCGTCACTTTAGTCCCTGAATGAAGAGAAATTGCAAAACAGTCCCTGCATGGTCAATTTCAGGGACTAAATTGACTAACGAAGTTTGCATTCAGGGACTAAATTGACTAACGAAGTTTGCATTCAGGGACTAAATTGACTAACAAAGTCTACATTCAGGGACTATTTTGCAATTTATCTGCATTCAGGGACTAAAGTGACGAATCCCTTCCGATTCAGGGACTAAAGTGACTAATTCCCCTAGATAATTATGAGCATGTATTGTTGTGACAGGTTGCTGGAAAGTTTGATGAGTCAAGATGTGGATTATACCGTGGCTCTTACTGCATTTTGGGCAATTGAAGTGGTGTATCAAGAGAGTTTTGCACACTGTATTGAAGAAGGCTCAAAAACACCACCAGAATTGAAGGAGACTTGTGAAAGATGGGGCAATGAAGGGTTTGGTCAGTACTGCCAATCTCTGCAAAAGATAGTCAATCAACGCTTGCAGAAGGCTTCTGATGATGAGCTAAAGAAGGCTGAAGTCATGTTGCTGAGTATTATTGAACATGAAGTTCATTTCTGGAATATGAGCCGTGGAAATGTTTGA

Protein sequence

>MS.gene061002.t1

MTETWLRKHRPIYDAATRHPFILSIRDGTVQSHSFKTWLAQDYLFVREFVPFVASVLIKACKESDDSDDVEVILGGMASLKDEILWFKREANKWGISLSDVVPQKPNKNYCRLLESLMSQDVDYTVALTAFWAIEVVYQESFAHCIEEGSKTPPELKETCERWGNEGFGQYCQSLQKIVNQRLQKASDDELKKAEVMLLSIIEHEVHFWNMSRGNV