AlfalfaGEDB | MS.gene063752

M. sativa cultivar XinJiangDaYe / MS.gene063752

KEGG: K18674
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063752.t1	XP_003609805.1	98.2	113	2	0	1	113	275	387	2.30E-50	208

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063752.t1	Q9SEE5	70.8	113	33	0	1	113	274	386	5.6e-37	154.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063752.t1	G7JNZ5	98.2	113	2	0	1	113	275	387	1.7e-50	208.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene063752	0.800253	1.57E-48	-1.69E-46
MS.gene049254	MS.gene063752	0.802332	5.90E-49	-1.69E-46
MS.gene049341	MS.gene063752	0.806488	8.03E-50	-1.69E-46
MS.gene049404	MS.gene063752	0.830636	2.63E-55	-1.69E-46
MS.gene049711	MS.gene063752	0.805977	1.03E-49	-1.69E-46
MS.gene049824	MS.gene063752	0.801112	1.05E-48	-1.69E-46
MS.gene049850	MS.gene063752	0.827081	1.90E-54	-1.69E-46
MS.gene05016	MS.gene063752	0.802176	6.35E-49	-1.69E-46
MS.gene050559	MS.gene063752	0.80721	5.65E-50	-1.69E-46
MS.gene050689	MS.gene063752	0.832429	9.50E-56	-1.69E-46
MS.gene050807	MS.gene063752	0.814958	1.18E-51	-1.69E-46
MS.gene051279	MS.gene063752	0.800268	1.56E-48	-1.69E-46
MS.gene051719	MS.gene063752	0.829887	4.00E-55	-1.69E-46
MS.gene051720	MS.gene063752	0.823254	1.53E-53	-1.69E-46
MS.gene052133	MS.gene063752	0.805017	1.64E-49	-1.69E-46
MS.gene052463	MS.gene063752	0.805564	1.26E-49	-1.69E-46
MS.gene052516	MS.gene063752	0.85776	1.31E-62	-1.69E-46
MS.gene052519	MS.gene063752	0.811333	7.38E-51	-1.69E-46
MS.gene053079	MS.gene063752	0.852794	3.65E-61	-1.69E-46
MS.gene053226	MS.gene063752	0.812377	4.37E-51	-1.69E-46
MS.gene053575	MS.gene063752	0.813578	2.38E-51	-1.69E-46
MS.gene053599	MS.gene063752	0.809538	1.80E-50	-1.69E-46
MS.gene05364	MS.gene063752	0.85879	6.47E-63	-1.69E-46
MS.gene053678	MS.gene063752	0.822844	1.91E-53	-1.69E-46
MS.gene054250	MS.gene063752	0.809048	2.29E-50	-1.69E-46
MS.gene054651	MS.gene063752	0.83303	6.74E-56	-1.69E-46
MS.gene055465	MS.gene063752	0.800113	1.68E-48	-1.69E-46
MS.gene055466	MS.gene063752	0.809148	2.18E-50	-1.69E-46
MS.gene055512	MS.gene063752	0.825301	5.05E-54	-1.69E-46
MS.gene055890	MS.gene063752	0.803921	2.77E-49	-1.69E-46
MS.gene055891	MS.gene063752	0.821367	4.19E-53	-1.69E-46
MS.gene05686	MS.gene063752	0.806507	7.96E-50	-1.69E-46
MS.gene057060	MS.gene063752	0.800119	1.67E-48	-1.69E-46
MS.gene057693	MS.gene063752	0.800816	1.21E-48	-1.69E-46
MS.gene057705	MS.gene063752	0.800074	1.71E-48	-1.69E-46
MS.gene058008	MS.gene063752	0.810715	1.00E-50	-1.69E-46
MS.gene058009	MS.gene063752	0.824237	9.00E-54	-1.69E-46
MS.gene058625	MS.gene063752	0.815229	1.03E-51	-1.69E-46
MS.gene05978	MS.gene063752	0.807066	6.06E-50	-1.69E-46
MS.gene060587	MS.gene063752	0.834056	3.74E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063752.t1	MTR_4g122670	98.230	113	2	0	1	113	275	387	3.29e-72	221
MS.gene063752.t1	MTR_4g122670	98.230	113	2	0	1	113	275	387	2.41e-71	221

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063752.t1	AT3G06580	70.796	113	33	0	1	113	274	386	1.46e-44	150

Find 20 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGATTTCTTCAGCTGTATA+TGG	0.299037	8.1:+8486794	None:intergenic
GAATATTAATGCATCTTACT+TGG	0.408856	8.1:-8486738	MS.gene063752:CDS
ACGTTGTATCATCAAATCTA+AGG	0.409282	8.1:-8486458	None:intergenic
GAAGAAATCGAAGAAGTTAC+CGG	0.424087	8.1:-8486782	MS.gene063752:CDS
GGGTTATGTGTATCATTTGC+TGG	0.433557	8.1:-8487047	MS.gene063752:CDS
CAAGAGGGTATATGCTTTCA+AGG	0.443777	8.1:-8486481	MS.gene063752:CDS
CTTGAAAGCATATACCCTCT+TGG	0.502513	8.1:+8486482	None:intergenic
AAAGCAATACAAGTTGCATC+AGG	0.513624	8.1:-8486699	MS.gene063752:intron
ATTACCTTCACAGCGAGTAC+AGG	0.528647	8.1:+8487005	None:intergenic
TGATCCTGTACTCGCTGTGA+AGG	0.552414	8.1:-8487009	MS.gene063752:intron
TACACATAACCCTTCAACAT+CGG	0.571247	8.1:+8487058	None:intergenic
ATTGTGCTCGCTATAAAGCT+AGG	0.575941	8.1:-8487122	MS.gene063752:CDS
CAACACTATCCGATGTTGAA+GGG	0.588728	8.1:-8487067	MS.gene063752:CDS
CGTGTATTCAGAAGCCAAGA+GGG	0.590972	8.1:-8486496	MS.gene063752:CDS
ACAACACTATCCGATGTTGA+AGG	0.595651	8.1:-8487068	MS.gene063752:CDS
AAGAAATCGAAGAAGTTACC+GGG	0.596482	8.1:-8486781	MS.gene063752:CDS
GCTAGGAATGGCACCAACTG+AGG	0.596765	8.1:-8487105	MS.gene063752:CDS
GCTCGCTATAAAGCTAGGAA+TGG	0.642563	8.1:-8487117	MS.gene063752:CDS
ACGTGTATTCAGAAGCCAAG+AGG	0.678724	8.1:-8486497	MS.gene063752:CDS
AGAAATCGAAGAAGTTACCG+GGG	0.742768	8.1:-8486780	MS.gene063752:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATTTAAATAGTAAAAGA+AGG	+	chr8.1:8486647-8486666	None:intergenic	10.0%
!!!	AAGATTTGAAATTTTTATGA+AGG	+	chr8.1:8486717-8486736	None:intergenic	15.0%
!!	AAATGGCTAAAAATTTATTG+TGG	-	chr8.1:8486961-8486980	MS.gene063752:intron	20.0%
!	ATACATGAAATCTTCACTAA+TGG	+	chr8.1:8486924-8486943	None:intergenic	25.0%
!	ATTAGTGAAGATTTCATGTA+TGG	-	chr8.1:8486923-8486942	MS.gene063752:intron	25.0%
!	GAATATTAATGCATCTTACT+TGG	-	chr8.1:8486843-8486862	MS.gene063752:intron	25.0%
!	TACATGAAATCTTCACTAAT+GGG	+	chr8.1:8486923-8486942	None:intergenic	25.0%
	TTCAAATATTCCTATAGCGA+AGG	+	chr8.1:8486760-8486779	None:intergenic	30.0%
!	TATTTCTGTTACAACTGATG+TGG	-	chr8.1:8486677-8486696	MS.gene063752:intron	30.0%
!!	CACAGAGTTAATTTTAAGAG+TGG	+	chr8.1:8486618-8486637	None:intergenic	30.0%
	AAAGCAATACAAGTTGCATC+AGG	-	chr8.1:8486882-8486901	MS.gene063752:intron	35.0%
	AAGAAATCGAAGAAGTTACC+GGG	-	chr8.1:8486800-8486819	MS.gene063752:CDS	35.0%
	ACATTAGCTGCTAAAACCAA+TGG	+	chr8.1:8487020-8487039	None:intergenic	35.0%
	GAAGAAATCGAAGAAGTTAC+CGG	-	chr8.1:8486799-8486818	MS.gene063752:CDS	35.0%
	TAAAAACGTTCCTTCGCTAT+AGG	-	chr8.1:8486747-8486766	MS.gene063752:CDS	35.0%
	TACACATAACCCTTCAACAT+CGG	+	chr8.1:8486526-8486545	None:intergenic	35.0%
	TCGATTTCTTCAGCTGTATA+TGG	+	chr8.1:8486790-8486809	None:intergenic	35.0%
!	TTTTAGCCATTTCCTAACTG+TGG	+	chr8.1:8486953-8486972	None:intergenic	35.0%
!!	AACCAATGGTAATGGTATGT+TGG	+	chr8.1:8487006-8487025	None:intergenic	35.0%
!!!	ACTTTTGAAATTGCCTCAGT+TGG	+	chr8.1:8486492-8486511	None:intergenic	35.0%
	ACAACACTATCCGATGTTGA+AGG	-	chr8.1:8486513-8486532	MS.gene063752:intron	40.0%
	AGAAATCGAAGAAGTTACCG+GGG	-	chr8.1:8486801-8486820	MS.gene063752:CDS	40.0%
	ATTGTGCTCGCTATAAAGCT+AGG	-	chr8.1:8486459-8486478	MS.gene063752:CDS	40.0%
	CAACACTATCCGATGTTGAA+GGG	-	chr8.1:8486514-8486533	MS.gene063752:intron	40.0%
	GCTGCTAAAACCAATGGTAA+TGG	+	chr8.1:8487014-8487033	None:intergenic	40.0%
	GCTGTGAAGGTAATTTAACC+TGG	-	chr8.1:8486585-8486604	MS.gene063752:intron	40.0%
	GGGTTATGTGTATCATTTGC+TGG	-	chr8.1:8486534-8486553	MS.gene063752:intron	40.0%
	TGCCAACATACCATTACCAT+TGG	-	chr8.1:8487001-8487020	MS.gene063752:intron	40.0%
!	AAATGTAGTCAGCTTTTCCC+CGG	+	chr8.1:8486821-8486840	None:intergenic	40.0%
!	CAAGAGGGTATATGCTTTCA+AGG	-	chr8.1:8487100-8487119	MS.gene063752:CDS	40.0%
!	CTTGAAAGCATATACCCTCT+TGG	+	chr8.1:8487102-8487121	None:intergenic	40.0%
!!	TGGTAATGGTATGTTGGCAA+TGG	+	chr8.1:8487000-8487019	None:intergenic	40.0%
	ACGTGTATTCAGAAGCCAAG+AGG	-	chr8.1:8487084-8487103	MS.gene063752:CDS	45.0%
	ATGTATGGCGTACCACAGTT+AGG	-	chr8.1:8486938-8486957	MS.gene063752:intron	45.0%
	ATTACCTTCACAGCGAGTAC+AGG	+	chr8.1:8486579-8486598	None:intergenic	45.0%
	CGTGTATTCAGAAGCCAAGA+GGG	-	chr8.1:8487085-8487104	MS.gene063752:CDS	45.0%
	GCTCGCTATAAAGCTAGGAA+TGG	-	chr8.1:8486464-8486483	MS.gene063752:CDS	45.0%
	TTTAAGAGTGGCATCACACC+AGG	+	chr8.1:8486606-8486625	None:intergenic	45.0%
!!!	TTTACTATTTAAATTTTATA+TGG	-	chr8.1:8486650-8486669	MS.gene063752:intron	5.0%
	GGCGTACCACAGTTAGGAAA+TGG	-	chr8.1:8486944-8486963	MS.gene063752:intron	50.0%
	TGATCCTGTACTCGCTGTGA+AGG	-	chr8.1:8486572-8486591	MS.gene063752:intron	50.0%
	GCTAGGAATGGCACCAACTG+AGG	-	chr8.1:8486476-8486495	MS.gene063752:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	8486459	8487144	8486459	ID=MS.gene063752
chr8.1	mRNA	8486459	8487144	8486459	ID=MS.gene063752.t1;Parent=MS.gene063752
chr8.1	exon	8487010	8487144	8487010	ID=MS.gene063752.t1.exon1;Parent=MS.gene063752.t1
chr8.1	CDS	8487010	8487144	8487010	ID=cds.MS.gene063752.t1;Parent=MS.gene063752.t1
chr8.1	exon	8486700	8486834	8486700	ID=MS.gene063752.t1.exon2;Parent=MS.gene063752.t1
chr8.1	CDS	8486700	8486834	8486700	ID=cds.MS.gene063752.t1;Parent=MS.gene063752.t1
chr8.1	exon	8486459	8486529	8486459	ID=MS.gene063752.t1.exon3;Parent=MS.gene063752.t1
chr8.1	CDS	8486459	8486529	8486459	ID=cds.MS.gene063752.t1;Parent=MS.gene063752.t1

Gene Sequence

>MS.gene063752

ATTGTGCTCGCTATAAAGCTAGGAATGGCACCAACTGAGGCAATTTCAAAAGTGACAACACTATCCGATGTTGAAGGGTTATGTGTATCATTTGCTGGTACTCGAAACTCATCTGATCCTGTACTCGCTGTGAAGGTAATTTAACCTGGTGTGATGCCACTCTTAAAATTAACTCTGTGAAACTTCCTTCTTTTACTATTTAAATTTTATATGGAGAATATTTCTGTTACAACTGATGTGGACATATGTTTTTTACCTTCATAAAAATTTCAAATCTTTTTCTGTTTATAAAAACGTTCCTTCGCTATAGGAATATTTGAAAGAAGAACCATATACAGCTGAAGAAATCGAAGAAGTTACCGGGGAAAAGCTGACTACATTTTTGAATATTAATGCATCTTACTTGGAAGTCATAAAAGCTGCAAAGCAATACAAGTTGCATCAGGTTTGTTTTTTTTAATCCCATTAGTGAAGATTTCATGTATGGCGTACCACAGTTAGGAAATGGCTAAAAATTTATTGTGGTTTTTGAGTTTTACCATTGCCAACATACCATTACCATTGGTTTTAGCAGCTAATGTATTGTTTCACTTTATGCTTTATTCGTGTTTGTAGAGAGCTGCTCACGTGTATTCAGAAGCCAAGAGGGTATATGCTTTCAAGGACGTTGTATCATCAAATCTAAG

Protein sequence

>MS.gene063752.t1

IVLAIKLGMAPTEAISKVTTLSDVEGLCVSFAGTRNSSDPVLAVKEYLKEEPYTAEEIEEVTGEKLTTFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNL