AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48483

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48483.t1 MTR_3g101370 93.810 420 4 1 1 398 1 420 0.0 810
MS.gene48483.t1 MTR_3g101370 90.816 294 5 1 1 272 1 294 0.0 547
MS.gene48483.t1 MTR_1g020080 66.590 434 100 9 1 398 1 425 0.0 530
MS.gene48483.t1 MTR_4g086600 59.736 303 92 6 68 351 124 415 1.42e-120 357
MS.gene48483.t1 MTR_1g020080 61.489 309 80 5 1 277 1 302 1.20e-117 345
MS.gene48483.t1 MTR_4g131970 51.977 354 152 9 41 388 52 393 2.66e-108 325
MS.gene48483.t1 MTR_2g022950 55.921 304 128 3 97 394 86 389 2.06e-104 314
MS.gene48483.t1 MTR_2g097960 52.475 303 116 5 97 372 41 342 2.36e-99 302
MS.gene48483.t1 MTR_2g022950 62.420 157 58 1 97 252 86 242 1.27e-62 202
MS.gene48483.t1 MTR_2g022950 62.420 157 58 1 97 252 86 242 7.20e-62 201
MS.gene48483.t1 MTR_3g109090 31.461 267 153 6 97 343 70 326 6.17e-32 125
MS.gene48483.t1 MTR_3g109090 31.461 267 153 6 97 343 86 342 6.61e-32 125
MS.gene48483.t1 MTR_4g124940 31.086 267 147 8 100 343 75 327 5.45e-29 117
MS.gene48483.t1 MTR_4g124940 25.269 186 113 4 166 345 47 212 4.55e-11 64.3
MS.gene48483.t1 MTR_8g022050 30.943 265 151 10 100 343 91 344 2.89e-27 112
MS.gene48483.t1 MTR_4g085540 27.969 261 172 6 97 349 148 400 3.04e-25 108
MS.gene48483.t1 MTR_4g085540 26.639 244 154 9 92 330 55 278 1.24e-17 85.1
MS.gene48483.t1 MTR_4g085540 31.429 175 98 4 166 335 35 192 3.84e-17 84.0
MS.gene48483.t1 MTR_1g019150 27.413 259 177 7 97 349 146 399 2.54e-21 96.7
MS.gene48483.t1 MTR_1g019150 25.410 244 157 7 92 330 53 276 2.88e-16 81.3
MS.gene48483.t1 MTR_1g097440 27.547 265 166 10 97 356 56 299 1.33e-19 91.3
MS.gene48483.t1 MTR_1g097440 26.000 250 160 9 97 333 144 381 2.19e-15 78.6
MS.gene48483.t1 MTR_6g040190 29.008 262 154 10 97 352 51 286 2.15e-19 90.5
MS.gene48483.t1 MTR_6g040190 21.786 280 194 8 83 347 117 386 3.12e-13 71.6
MS.gene48483.t1 MTR_6g040190 27.933 179 107 4 166 339 26 187 3.38e-13 71.6
MS.gene48483.t1 MTR_7g102730 24.715 263 171 8 90 347 31 271 3.18e-19 90.1
MS.gene48483.t1 MTR_7g102730 27.197 239 158 6 97 327 124 354 2.46e-17 84.3
MS.gene48483.t1 MTR_7g102730 28.090 178 100 4 166 335 11 168 4.42e-14 74.3
MS.gene48483.t1 MTR_3g102040 24.555 281 192 7 83 349 116 390 1.04e-17 85.5
MS.gene48483.t1 MTR_3g102040 31.429 175 98 4 166 335 25 182 5.77e-16 80.1
MS.gene48483.t1 MTR_3g102040 24.746 295 191 9 65 352 16 286 7.77e-16 79.7
MS.gene48483.t1 MTR_1g061870 24.219 256 169 7 97 347 41 276 1.25e-17 85.1
MS.gene48483.t1 MTR_1g061870 29.213 178 104 4 163 335 13 173 1.91e-14 75.1
MS.gene48483.t1 MTR_1g061870 25.207 242 157 8 97 328 129 356 4.70e-11 64.7
MS.gene48483.t1 MTR_6g040070 23.675 283 192 9 83 349 19 293 5.12e-16 80.5
MS.gene48483.t1 MTR_6g040070 29.592 196 117 6 166 356 16 195 2.16e-14 75.5
MS.gene48483.t1 MTR_3g065190 28.000 175 114 4 97 271 51 213 2.05e-15 77.4
MS.gene48483.t1 MTR_4g104470 26.943 193 120 4 164 348 121 300 3.15e-15 76.3
MS.gene48483.t1 MTR_4g104470 31.447 159 86 6 101 247 152 299 8.62e-14 72.0
MS.gene48483.t1 MTR_7g107580 24.615 260 156 8 153 389 4 246 5.39e-14 73.2
MS.gene48483.t1 MTR_1g080150 36.782 87 54 1 164 250 131 216 8.64e-14 70.9
MS.gene48483.t1 MTR_3g075500 29.870 154 100 3 101 249 139 289 1.02e-13 71.6
MS.gene48483.t1 MTR_7g107580 24.615 260 156 8 153 389 4 246 1.11e-13 72.8
MS.gene48483.t1 MTR_4g068120 23.810 189 116 3 153 341 15 175 1.95e-13 72.8
MS.gene48483.t1 MTR_1g064230 27.103 214 138 6 150 348 70 280 3.30e-13 69.7
MS.gene48483.t1 MTR_7g118120 21.790 257 171 6 97 347 130 362 3.37e-13 71.2
MS.gene48483.t1 MTR_7g107580 24.138 261 157 8 153 389 4 247 6.67e-13 70.1
MS.gene48483.t1 MTR_3g079200 41.558 77 44 1 266 341 224 300 4.86e-12 67.8
MS.gene48483.t1 MTR_7g094660 25.121 207 138 5 160 357 636 834 1.33e-11 67.0
MS.gene48483.t1 MTR_3g075500 30.597 134 85 3 101 229 139 269 2.02e-11 64.3
MS.gene48483.t1 MTR_3g103420 27.374 179 112 5 167 330 12 187 3.71e-11 64.7
MS.gene48483.t1 MTR_1g045510 31.126 151 94 5 100 247 142 285 4.70e-11 63.5
MS.gene48483.t1 MTR_1g056520 27.811 169 111 4 97 257 134 299 8.79e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48483.t1 AT1G47490 61.347 401 114 11 30 398 41 432 5.45e-163 464
MS.gene48483.t1 AT1G47500 61.719 384 116 6 44 398 53 434 1.88e-155 446
MS.gene48483.t1 AT3G19130 52.078 409 144 6 1 358 1 408 1.21e-131 385
MS.gene48483.t1 AT1G49600 59.878 329 95 7 86 383 112 434 6.82e-131 383
MS.gene48483.t1 AT1G49600 61.409 298 83 4 86 352 112 408 5.04e-125 369
MS.gene48483.t1 AT1G49600 61.409 298 83 4 86 352 112 408 5.04e-125 369
MS.gene48483.t1 AT1G11650 48.663 374 139 9 44 376 2 363 1.61e-103 312
MS.gene48483.t1 AT5G54900 51.724 319 117 6 88 377 55 365 4.04e-97 295
MS.gene48483.t1 AT4G27000 57.028 249 106 1 99 347 108 355 4.47e-94 288
MS.gene48483.t1 AT1G47490 54.909 275 87 8 30 274 41 308 4.09e-92 280
MS.gene48483.t1 AT1G47490 28.125 192 116 7 156 337 92 271 4.73e-13 69.7
MS.gene48483.t1 AT5G19350 46.667 345 139 8 84 393 15 349 2.61e-90 279
MS.gene48483.t1 AT5G19350 45.845 349 140 8 84 393 15 353 1.98e-89 276
MS.gene48483.t1 AT1G11650 48.232 311 116 6 44 315 2 306 4.18e-86 264
MS.gene48483.t1 AT1G47500 54.475 257 90 3 44 273 53 309 1.44e-83 258
MS.gene48483.t1 AT1G47500 26.702 191 120 5 156 337 94 273 2.59e-11 64.7
MS.gene48483.t1 AT1G54080 35.206 267 143 11 97 343 89 345 5.72e-33 128
MS.gene48483.t1 AT1G54080 25.946 185 111 5 166 344 64 228 1.67e-11 65.9
MS.gene48483.t1 AT1G54080 34.686 271 143 12 97 343 89 349 2.10e-31 124
MS.gene48483.t1 AT1G54080 26.455 189 109 5 166 344 64 232 3.72e-11 64.7
MS.gene48483.t1 AT1G17370 32.331 266 149 10 97 343 80 333 2.86e-30 120
MS.gene48483.t1 AT1G17370 32.210 267 151 10 97 343 80 336 3.81e-30 120
MS.gene48483.t1 AT3G14100 32.584 267 150 9 97 343 85 341 7.38e-30 120
MS.gene48483.t1 AT3G14100 25.946 185 111 5 166 344 60 224 1.07e-11 66.6
MS.gene48483.t1 AT1G71770 25.448 279 190 7 83 347 150 424 2.00e-23 102
MS.gene48483.t1 AT1G71770 24.074 270 175 9 92 352 80 328 7.59e-19 89.0
MS.gene48483.t1 AT1G71770 25.448 279 190 7 83 347 150 424 2.00e-23 102
MS.gene48483.t1 AT1G71770 24.074 270 175 9 92 352 80 328 7.59e-19 89.0
MS.gene48483.t1 AT2G23350 27.632 304 184 11 83 364 138 427 6.84e-22 98.2
MS.gene48483.t1 AT2G23350 29.714 175 101 3 166 335 47 204 6.90e-15 77.0
MS.gene48483.t1 AT2G23350 24.653 288 178 10 93 369 68 327 8.04e-15 76.6
MS.gene48483.t1 AT4G34110 27.586 261 173 7 97 349 150 402 7.79e-22 98.2
MS.gene48483.t1 AT4G34110 27.426 237 145 7 97 327 62 277 5.99e-20 92.4
MS.gene48483.t1 AT4G34110 33.143 175 95 4 166 335 37 194 6.87e-18 86.3
MS.gene48483.t1 AT1G22760 24.911 281 189 8 83 347 140 414 1.01e-21 97.8
MS.gene48483.t1 AT1G22760 24.715 263 172 10 97 352 75 318 1.17e-17 85.5
MS.gene48483.t1 AT1G22760 30.337 178 101 5 163 335 47 206 4.75e-13 71.2
MS.gene48483.t1 AT1G49760 24.138 261 182 5 97 349 159 411 3.46e-19 90.1
MS.gene48483.t1 AT1G49760 33.714 175 94 4 166 335 46 203 7.44e-19 89.0
MS.gene48483.t1 AT1G49760 28.033 239 145 8 97 329 71 288 1.50e-15 79.0
MS.gene48483.t1 AT1G49760 24.138 261 182 5 97 349 159 411 3.46e-19 90.1
MS.gene48483.t1 AT1G49760 33.714 175 94 4 166 335 46 203 7.44e-19 89.0
MS.gene48483.t1 AT1G49760 28.033 239 145 8 97 329 71 288 1.50e-15 79.0
MS.gene48483.t1 AT1G34140 25.850 294 188 10 83 359 35 315 1.06e-18 87.8
MS.gene48483.t1 AT3G16380 27.547 265 174 8 97 352 138 393 3.46e-17 84.0
MS.gene48483.t1 AT3G16380 28.796 191 104 5 150 330 8 176 2.63e-11 65.5
MS.gene48483.t1 AT2G36660 21.673 263 177 8 97 352 50 290 8.39e-15 76.6
MS.gene48483.t1 AT2G36660 24.609 256 172 8 110 354 150 395 1.54e-14 75.9
MS.gene48483.t1 AT2G36660 27.429 175 105 4 166 335 25 182 3.07e-13 71.6
MS.gene48483.t1 AT4G14300 29.885 174 115 2 167 334 8 180 1.34e-13 72.4
MS.gene48483.t1 AT4G14300 29.885 174 115 2 167 334 8 180 1.34e-13 72.4
MS.gene48483.t1 AT4G24770 31.973 147 95 3 101 245 180 323 1.56e-13 71.6
MS.gene48483.t1 AT4G24770 31.973 147 95 3 101 245 180 323 1.56e-13 71.6
MS.gene48483.t1 AT3G04500 33.028 109 67 2 164 272 136 238 1.74e-13 70.1
MS.gene48483.t1 AT3G52380 27.950 161 102 3 97 246 142 299 5.26e-13 70.1
MS.gene48483.t1 AT2G37220 25.789 190 125 4 164 338 90 278 6.98e-13 68.9
MS.gene48483.t1 AT2G18510 25.714 175 118 4 97 271 51 213 1.04e-12 69.3
MS.gene48483.t1 AT1G58470 27.273 253 146 9 167 388 8 253 1.53e-12 68.9
MS.gene48483.t1 AT1G58470 27.273 253 146 9 167 388 8 253 1.53e-12 68.9
MS.gene48483.t1 AT1G07360 38.272 81 49 1 262 341 220 300 3.54e-12 68.2
MS.gene48483.t1 AT3G13224 26.519 181 111 5 167 340 21 186 4.31e-12 65.9
MS.gene48483.t1 AT5G50250 27.119 177 112 4 167 337 115 280 4.39e-12 66.6
MS.gene48483.t1 AT5G50250 31.293 147 96 3 101 245 143 286 1.28e-11 65.5
MS.gene48483.t1 AT2G29580 38.272 81 49 1 262 341 220 300 7.34e-12 67.4
MS.gene48483.t1 AT3G13224 25.424 177 111 4 167 337 12 173 7.62e-12 66.6
MS.gene48483.t1 AT3G13224 25.424 177 111 4 167 337 21 182 8.22e-12 66.6
MS.gene48483.t1 AT4G00830 21.154 260 168 7 97 347 142 373 1.18e-11 66.6
MS.gene48483.t1 AT4G00830 21.154 260 168 7 97 347 142 373 1.18e-11 66.6
MS.gene48483.t1 AT4G00830 21.154 260 168 7 97 347 142 373 1.18e-11 66.6
MS.gene48483.t1 AT1G60000 29.677 155 90 5 100 246 114 257 1.20e-11 65.1
MS.gene48483.t1 AT4G13850 38.272 81 49 1 167 247 37 116 2.36e-11 61.2
MS.gene48483.t1 AT2G33410 27.011 174 116 3 167 330 8 180 6.95e-11 63.9

Find 113 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTATTGCGAATAACCTTAA+TGG 0.180225 3.1:+80493048 None:intergenic
TGGAACGGGCCAGCATATTA+TGG 0.217568 3.1:-80491222 MS.gene48483:CDS
AGCAAACAGTTCGTCTTTCT+TGG 0.248297 3.1:-80491706 MS.gene48483:CDS
GCAAACAGTTCGTCTTTCTT+GGG 0.324805 3.1:-80491705 MS.gene48483:CDS
ATAATATGCTGGCCCGTTCC+AGG 0.343614 3.1:+80491224 None:intergenic
GAAGCATTGCAGAAGCTAAA+TGG 0.357684 3.1:-80491743 MS.gene48483:CDS
ACAATTACCTGCTTATTTGC+CGG 0.361642 3.1:+80491674 None:intergenic
CAGAGACTATCTCACCATAC+TGG 0.369249 3.1:+80491936 None:intergenic
CTGTTGGTGTCCTCCATACA+TGG 0.369434 3.1:+80491125 None:intergenic
ACCGGACGTTCAAAGGGTTA+TGG 0.372671 3.1:-80492737 MS.gene48483:CDS
GGTTATGGTTTCGTCAGGTT+TGG 0.373844 3.1:-80492722 MS.gene48483:CDS
ACCCTTTGAACGTCCGGTAT+TGG 0.373855 3.1:+80492742 None:intergenic
GGCTGGGTGTGCCGTTTGAT+TGG 0.374274 3.1:+80492123 None:intergenic
CTGCTGATAATAAAACTCTC+TGG 0.376726 3.1:-80494182 MS.gene48483:CDS
GATGCATTATAGATATTTGT+TGG 0.384978 3.1:-80492007 MS.gene48483:intron
TCTTTCTTGGGGCCGCAATC+CGG 0.397217 3.1:-80491693 MS.gene48483:CDS
GGACCTGCTTATGATGGTTA+TGG 0.398241 3.1:-80491198 MS.gene48483:CDS
TGCTAAATGTCGCCCAGTTA+AGG 0.409625 3.1:+80492909 None:intergenic
CGAAAAGGTTGCTCTGTGTT+AGG 0.415515 3.1:+80492932 None:intergenic
GGAACGGGCCAGCATATTAT+GGG 0.418242 3.1:-80491221 MS.gene48483:CDS
CAAAGGGTTATGGTTTCGTC+AGG 0.421280 3.1:-80492727 MS.gene48483:CDS
AATTCGGCAGTCCCTGGAAC+GGG 0.437944 3.1:-80491236 MS.gene48483:CDS
TCATTTAGCTTACTTGCTGT+TGG 0.448711 3.1:+80491109 None:intergenic
AGACAACCATTCTCCCAGTA+TGG 0.449229 3.1:-80491950 MS.gene48483:CDS
GGAACCCACTGGTGCGGTGG+TGG 0.454001 3.1:+80494342 None:intergenic
TCTAGCAGGGCAATGCGAAT+TGG 0.457296 3.1:-80492647 MS.gene48483:CDS
GCATACATGCTCTGGTCATA+AGG 0.457460 3.1:+80491159 None:intergenic
ACCATAACCCTTTGAACGTC+CGG 0.459242 3.1:+80492736 None:intergenic
AGAATCAGCATCAGATTGGC+TGG 0.462991 3.1:+80492106 None:intergenic
AACAGAGGGGTATGGATTTG+TGG 0.467857 3.1:-80493014 MS.gene48483:CDS
TGCCAATACCGGACGTTCAA+AGG 0.470787 3.1:-80492744 MS.gene48483:CDS
GCTGCTGCTTACCCCATGTA+TGG 0.473026 3.1:-80491138 MS.gene48483:CDS
TAGCAGTTTAGATCTGAATT+CGG 0.474047 3.1:-80491252 MS.gene48483:intron
GTTAAGATACCTGTTGGAAA+AGG 0.477975 3.1:-80491914 MS.gene48483:CDS
GATCTGAATTCGGCAGTCCC+TGG 0.480859 3.1:-80491242 MS.gene48483:CDS
GCCAATACCGGACGTTCAAA+GGG 0.484393 3.1:-80492743 MS.gene48483:CDS
TTGTTATTACTCTGCTCCGT+TGG 0.489383 3.1:+80494417 None:intergenic
TTCGTCAGGTTTGGAGATGA+TGG 0.490103 3.1:-80492713 MS.gene48483:CDS
AGATCTTCAGCGGTAGCACT+AGG 0.494702 3.1:+80491974 None:intergenic
GTCTCTGTTAAGATACCTGT+TGG 0.495294 3.1:-80491920 MS.gene48483:CDS
CGGAGCAGAGTAATAACAAC+AGG 0.497406 3.1:-80494413 MS.gene48483:CDS
CACGTGCCTCACGTGCATCA+TGG 0.499582 3.1:-80494213 MS.gene48483:CDS
GCAATATCCTCCGGCGGCAA+TGG 0.502503 3.1:-80494316 MS.gene48483:CDS
ATCTCACCATACTGGGAGAA+TGG 0.509590 3.1:+80491944 None:intergenic
AAGCAGCAGCATACATGCTC+TGG 0.510563 3.1:+80491151 None:intergenic
GCCGCCACCACACCATTACA+TGG 0.516888 3.1:-80494256 MS.gene48483:CDS
TGATAGCCAGAGGATTTCCT+AGG 0.520577 3.1:+80492614 None:intergenic
AAGCTAAATGGTACAACAGT+TGG 0.520807 3.1:-80491731 MS.gene48483:CDS
CCTGCTTATTTGCCGGATTG+CGG 0.521827 3.1:+80491681 None:intergenic
CCGCAATCCGGCAAATAAGC+AGG 0.521834 3.1:-80491681 MS.gene48483:intron
TTGTAGAATCAGCATCAGAT+TGG 0.522042 3.1:+80492102 None:intergenic
GTCGCCCAGTTAAGGCGAAA+AGG 0.523550 3.1:+80492917 None:intergenic
ATACCTGTTGGAAAAGGATG+TGG 0.524194 3.1:-80491908 MS.gene48483:CDS
CAAACAGTTCGTCTTTCTTG+GGG 0.528594 3.1:-80491704 MS.gene48483:CDS
GAATTGGTGCTGCAACTCCT+AGG 0.534133 3.1:-80492631 MS.gene48483:CDS
ACTTGTCAAACAGAGGGGTA+TGG 0.546421 3.1:-80493022 MS.gene48483:CDS
CTTTAGCAGCTTTAACTGAA+GGG 0.546481 3.1:+80492777 None:intergenic
TGCTGATAATAAAACTCTCT+GGG 0.549803 3.1:-80494181 MS.gene48483:intron
TAATATGCTGGCCCGTTCCA+GGG 0.552273 3.1:+80491225 None:intergenic
CAGCGGTAGCACTAGGGTCA+AGG 0.553945 3.1:+80491981 None:intergenic
ACTTTAGCAGCTTTAACTGA+AGG 0.557323 3.1:+80492776 None:intergenic
TATACAGGAGGCCAATCAAA+CGG 0.557602 3.1:-80492134 MS.gene48483:intron
TATCCATAACCATCATAAGC+AGG 0.565694 3.1:+80491195 None:intergenic
GCCATGTAATGGTGTGGTGG+CGG 0.567433 3.1:+80494255 None:intergenic
GGCATTACCATTGCCGCCGG+AGG 0.570236 3.1:+80494309 None:intergenic
GCAACTCCTAGGAAATCCTC+TGG 0.574768 3.1:-80492620 MS.gene48483:CDS
TTTGTGGAATTCACTTCACA+TGG 0.575027 3.1:-80492998 MS.gene48483:CDS
AGAGACTATCTCACCATACT+GGG 0.577197 3.1:+80491937 None:intergenic
GTGAAGATGCAATCAACAAA+CGG 0.577977 3.1:-80494453 None:intergenic
GCATTATAGATATTTGTTGG+AGG 0.579075 3.1:-80492004 MS.gene48483:intron
GATATTGCATCGGAACCCAC+TGG 0.585730 3.1:+80494331 None:intergenic
ATGTAATGGTGTGGTGGCGG+CGG 0.586592 3.1:+80494258 None:intergenic
TGCGGCATTACCATTGCCGC+CGG 0.588023 3.1:+80494306 None:intergenic
CAGTTCCACCACCGCACCAG+TGG 0.589533 3.1:-80494347 MS.gene48483:CDS
GAATTCGGCAGTCCCTGGAA+CGG 0.589778 3.1:-80491237 MS.gene48483:CDS
AAGCAGACTTGTCAAACAGA+GGG 0.590205 3.1:-80493028 MS.gene48483:CDS
CATACCTTGTTGATAGCCAG+AGG 0.594509 3.1:+80492604 None:intergenic
AATGATGATAAGCCATGTAA+TGG 0.595373 3.1:+80494244 None:intergenic
GGTTCCGATGCAATATCCTC+CGG 0.597378 3.1:-80494325 MS.gene48483:CDS
AGTGAATGCGGTGAAGGTGG+TGG 0.598487 3.1:+80494372 None:intergenic
AACTGGGCGACATTTAGCAC+AGG 0.599909 3.1:-80492905 MS.gene48483:CDS
TGTTGGTGTCCTCCATACAT+GGG 0.602431 3.1:+80491126 None:intergenic
ATGCTCTGGTCATAAGGATG+CGG 0.606205 3.1:+80491165 None:intergenic
AAAGCTTTGAATGAAATGAA+TGG 0.607445 3.1:-80492680 MS.gene48483:CDS
GAATGCGGTGAAGGTGGTGG+CGG 0.607461 3.1:+80494375 None:intergenic
ATCGGAACCCACTGGTGCGG+TGG 0.611576 3.1:+80494339 None:intergenic
GAATCAGCATCAGATTGGCT+GGG 0.616222 3.1:+80492107 None:intergenic
GATCTTCAGCGGTAGCACTA+GGG 0.619503 3.1:+80491975 None:intergenic
ACTGTAAGTGAATGCGGTGA+AGG 0.629575 3.1:+80494366 None:intergenic
GGTTTGGAGATGATGGTGAA+AGG 0.630977 3.1:-80492706 MS.gene48483:CDS
TGCATCGGAACCCACTGGTG+CGG 0.636482 3.1:+80494336 None:intergenic
AAATCCTCTGGCTATCAACA+AGG 0.639178 3.1:-80492608 MS.gene48483:intron
GCCGCCGGAGGATATTGCAT+CGG 0.640739 3.1:+80494321 None:intergenic
CGGTTCTGATTCGAAACCAA+CGG 0.648196 3.1:-80494433 MS.gene48483:CDS
GCTGCTTACCCCATGTATGG+AGG 0.652288 3.1:-80491135 MS.gene48483:CDS
CTAGATCCATGATGCACGTG+AGG 0.657062 3.1:+80494207 None:intergenic
GGTGGCGGCGGATAATGTTG+AGG 0.657751 3.1:+80494270 None:intergenic
GTAAGTGAATGCGGTGAAGG+TGG 0.663508 3.1:+80494369 None:intergenic
TTGTGGAATTCACTTCACAT+GGG 0.663803 3.1:-80492997 MS.gene48483:CDS
TCCGATGCAATATCCTCCGG+CGG 0.665723 3.1:-80494322 MS.gene48483:CDS
AGTTCCACCACCGCACCAGT+GGG 0.669547 3.1:-80494346 MS.gene48483:CDS
GAACGGGCCAGCATATTATG+GGG 0.671150 3.1:-80491220 MS.gene48483:CDS
TAAGCAGACTTGTCAAACAG+AGG 0.678097 3.1:-80493029 MS.gene48483:CDS
TGGTTGTCTAAGATCTTCAG+CGG 0.682409 3.1:+80491964 None:intergenic
GGCGGCGGATAATGTTGAGG+CGG 0.682977 3.1:+80494273 None:intergenic
GTTGGTGTCCTCCATACATG+GGG 0.686565 3.1:+80491127 None:intergenic
TGAGGCGGTAACATATGATG+CGG 0.688591 3.1:+80494288 None:intergenic
AACGGGCCAGCATATTATGG+GGG 0.691722 3.1:-80491219 MS.gene48483:CDS
CATGATGCACGTGAGGCACG+TGG 0.702148 3.1:+80494214 None:intergenic
AGCAGACTTGTCAAACAGAG+GGG 0.704079 3.1:-80493027 MS.gene48483:CDS
GGTGGAACTGTAAGTGAATG+CGG 0.709974 3.1:+80494360 None:intergenic
TAAGCCATGTAATGGTGTGG+TGG 0.713782 3.1:+80494252 None:intergenic
TGATAAGCCATGTAATGGTG+TGG 0.730712 3.1:+80494249 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTATTAAGTAAATTTTTT+GGG - chr3.1:80491682-80491701 MS.gene48483:CDS 10.0%
!!! TTGTTATTAAGTAAATTTTT+TGG - chr3.1:80491681-80491700 MS.gene48483:intron 10.0%
!! AAATGTTGTGGTATTTATTT+AGG - chr3.1:80492380-80492399 MS.gene48483:intron 20.0%
!! AATAAATGCAGAGTAAAAAT+TGG + chr3.1:80493712-80493731 None:intergenic 20.0%
!! ACAAAAAGAAAGAAAGAAAA+AGG + chr3.1:80491483-80491502 None:intergenic 20.0%
!! ATAATCTCGATTGAATATTA+CGG + chr3.1:80492346-80492365 None:intergenic 20.0%
!! CAAAAAGAAAGAAAGAAAAA+GGG + chr3.1:80491482-80491501 None:intergenic 20.0%
!! TAAATACCACAACATTTATT+TGG + chr3.1:80492377-80492396 None:intergenic 20.0%
!! TTTAATCCAAATAAATGTTG+TGG - chr3.1:80492368-80492387 MS.gene48483:intron 20.0%
!!! TAAATGTATGCTTTGTTTTA+TGG - chr3.1:80493156-80493175 MS.gene48483:intron 20.0%
!!! TTTAGTTTGTTTCTTTATAC+AGG - chr3.1:80493411-80493430 MS.gene48483:intron 20.0%
! AAAGAAAGAAAAAGGGAAAA+GGG + chr3.1:80491475-80491494 None:intergenic 25.0%
! CAAAAAACTATTAATGCACA+TGG + chr3.1:80491975-80491994 None:intergenic 25.0%
! CATTCATTAAATGATGTATG+TGG - chr3.1:80491806-80491825 MS.gene48483:intron 25.0%
! CTACAAAAATAGAAAGATCA+GGG + chr3.1:80492705-80492724 None:intergenic 25.0%
! GATGCATTATAGATATTTGT+TGG - chr3.1:80493553-80493572 MS.gene48483:intron 25.0%
! GATTGTAAGTTTATGGAAAA+TGG - chr3.1:80491588-80491607 MS.gene48483:intron 25.0%
! GCATTTATTATGAGATAACA+AGG - chr3.1:80493723-80493742 MS.gene48483:intron 25.0%
! GTTCTTTGATTGTAAGTTTA+TGG - chr3.1:80491581-80491600 MS.gene48483:intron 25.0%
! TAAGCAAAAATCAACATTCT+AGG - chr3.1:80491781-80491800 MS.gene48483:intron 25.0%
!! AATCTGTCACTAATTTTGTA+AGG - chr3.1:80493531-80493550 MS.gene48483:intron 25.0%
!! AGAAAAAGTTTCAAGTAACA+TGG + chr3.1:80492755-80492774 None:intergenic 25.0%
!! AGAATCAAGTTTTCTCATTT+TGG - chr3.1:80492403-80492422 MS.gene48483:intron 25.0%
!! TTAAGCATGTATTTGAAACT+GGG - chr3.1:80492197-80492216 MS.gene48483:intron 25.0%
!! TTTAAGCATGTATTTGAAAC+TGG - chr3.1:80492196-80492215 MS.gene48483:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - chr3.1:80491880-80491899 MS.gene48483:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + chr3.1:80491899-80491880 None:intergenic 25.0%
!!! AAAGCTTTGAATGAAATGAA+TGG - chr3.1:80492880-80492899 MS.gene48483:CDS 25.0%
!!! ATGAATTTTGAAAGCTTAGT+GGG - chr3.1:80492133-80492152 MS.gene48483:intron 25.0%
!!! ATTGCAATAATGTCACTTTT+TGG + chr3.1:80492075-80492094 None:intergenic 25.0%
!!! ATTTTTTCACCAAACAAATG+TGG + chr3.1:80492107-80492126 None:intergenic 25.0%
!!! CATGTGCATTAATAGTTTTT+TGG - chr3.1:80491973-80491992 MS.gene48483:CDS 25.0%
!!! GCTTTGTTTTATGGTATAAT+TGG - chr3.1:80493165-80493184 MS.gene48483:intron 25.0%
!!! TATGAATTTTGAAAGCTTAG+TGG - chr3.1:80492132-80492151 MS.gene48483:intron 25.0%
!!! TATTTTTGTCGAGAAACTTA+TGG - chr3.1:80493111-80493130 MS.gene48483:intron 25.0%
!!! TCTCATTTTGGTAGTAAAAA+TGG - chr3.1:80492415-80492434 MS.gene48483:intron 25.0%
!!! TTGCAATAATGTCACTTTTT+GGG + chr3.1:80492074-80492093 None:intergenic 25.0%
AAGGTCTCTATTGATCTATA+GGG - chr3.1:80493954-80493973 MS.gene48483:intron 30.0%
AATGATGATAAGCCATGTAA+TGG + chr3.1:80491319-80491338 None:intergenic 30.0%
AATGTGGTCTACATTCAATT+CGG - chr3.1:80493034-80493053 MS.gene48483:CDS 30.0%
AGAGTGAAATTTCAGATATG+AGG + chr3.1:80492997-80493016 None:intergenic 30.0%
AGCATATGCAAATGTTGAAA+TGG + chr3.1:80493336-80493355 None:intergenic 30.0%
AGGAGAAATAAACAAGTGAA+TGG + chr3.1:80493268-80493287 None:intergenic 30.0%
ATGAAGTTCATGGTATTACT+AGG + chr3.1:80494009-80494028 None:intergenic 30.0%
CATGTAGTAGAAAAGAAATG+TGG - chr3.1:80493018-80493037 MS.gene48483:CDS 30.0%
CCTACAAAAATAGAAAGATC+AGG + chr3.1:80492706-80492725 None:intergenic 30.0%
CTTATTGCGAATAACCTTAA+TGG + chr3.1:80492515-80492534 None:intergenic 30.0%
GAAAGAAAGAAAAAGGGAAA+AGG + chr3.1:80491476-80491495 None:intergenic 30.0%
GAGAAATCACCATAAGAAAT+CGG + chr3.1:80492279-80492298 None:intergenic 30.0%
TAAAACCTACATGAAGTTCA+TGG + chr3.1:80494019-80494038 None:intergenic 30.0%
TAATACCATGAACTTCATGT+AGG - chr3.1:80494011-80494030 MS.gene48483:intron 30.0%
TAGCAGTTTAGATCTGAATT+CGG - chr3.1:80494308-80494327 MS.gene48483:CDS 30.0%
TGCTGATAATAAAACTCTCT+GGG - chr3.1:80491379-80491398 MS.gene48483:intron 30.0%
TTCTATCATAAGCATGTGTT+TGG + chr3.1:80492318-80492337 None:intergenic 30.0%
! ACGTATTAACCGATTTCTTA+TGG - chr3.1:80492267-80492286 MS.gene48483:intron 30.0%
! AGTTTGTTTCTTTATACAGG+AGG - chr3.1:80493414-80493433 MS.gene48483:intron 30.0%
! ATAAACAAGTGAATGGATTC+AGG + chr3.1:80493261-80493280 None:intergenic 30.0%
! ATGAACTTCATGTAGGTTTT+AGG - chr3.1:80494018-80494037 MS.gene48483:intron 30.0%
! CTTTTTATGCCACATTTGTT+TGG - chr3.1:80492095-80492114 MS.gene48483:CDS 30.0%
! GATAATTTTCATCCATCCAA+TGG + chr3.1:80491418-80491437 None:intergenic 30.0%
! GCATTATAGATATTTGTTGG+AGG - chr3.1:80493556-80493575 MS.gene48483:intron 30.0%
! GTTTTGTACAATTTGCAAAC+AGG - chr3.1:80493674-80493693 MS.gene48483:intron 30.0%
! TGTCGTTTCTACTTAAGATT+CGG - chr3.1:80491622-80491641 MS.gene48483:intron 30.0%
! TTTGCAGATTTTCTCCATTA+AGG - chr3.1:80492498-80492517 MS.gene48483:intron 30.0%
!! TGAATTTTGAAAGCTTAGTG+GGG - chr3.1:80492134-80492153 MS.gene48483:intron 30.0%
!!! AAAAAGTTCCCTTTTTTCTG+AGG + chr3.1:80491720-80491739 None:intergenic 30.0%
!!! AAAAGTTCCCTTTTTTCTGA+GGG + chr3.1:80491719-80491738 None:intergenic 30.0%
!!! CCTGATCTTTCTATTTTTGT+AGG - chr3.1:80492703-80492722 MS.gene48483:CDS 30.0%
!!! TAGGTTTTAGGAGAAACAAA+AGG - chr3.1:80494030-80494049 MS.gene48483:intron 30.0%
AAATGTTTGCACTTCTGACA+TGG - chr3.1:80492012-80492031 MS.gene48483:intron 35.0%
AAGCTAAATGGTACAACAGT+TGG - chr3.1:80493829-80493848 MS.gene48483:intron 35.0%
AATGTTTGCACTTCTGACAT+GGG - chr3.1:80492013-80492032 MS.gene48483:intron 35.0%
ACAATTACCTGCTTATTTGC+CGG + chr3.1:80493889-80493908 None:intergenic 35.0%
AGGTCTCTATTGATCTATAG+GGG - chr3.1:80493955-80493974 MS.gene48483:intron 35.0%
ATAGACAGAATGAGACAATC+TGG + chr3.1:80494237-80494256 None:intergenic 35.0%
CAAGGTCTCTATTGATCTAT+AGG - chr3.1:80493953-80493972 MS.gene48483:intron 35.0%
CTGCTGATAATAAAACTCTC+TGG - chr3.1:80491378-80491397 MS.gene48483:intron 35.0%
GAATTCAGATCTAAACTGCT+AGG + chr3.1:80494309-80494328 None:intergenic 35.0%
GATAATAAAACTCTCTGGGT+CGG - chr3.1:80491383-80491402 MS.gene48483:intron 35.0%
GGTATTACTAGGTGAGTTAT+TGG + chr3.1:80493998-80494017 None:intergenic 35.0%
GTGTTAACCCTCAGAAAAAA+GGG - chr3.1:80491709-80491728 MS.gene48483:CDS 35.0%
GTTAAGATACCTGTTGGAAA+AGG - chr3.1:80493646-80493665 MS.gene48483:intron 35.0%
TACTAATTGTGTACATCCTC+CGG - chr3.1:80491749-80491768 MS.gene48483:CDS 35.0%
TAGATCAATAGAGACCTTGT+AGG + chr3.1:80493952-80493971 None:intergenic 35.0%
TATCCATAACCATCATAAGC+AGG + chr3.1:80494368-80494387 None:intergenic 35.0%
TGGTTGTTGAGATTCTCATT+TGG - chr3.1:80492435-80492454 MS.gene48483:intron 35.0%
TGTGTTAACCCTCAGAAAAA+AGG - chr3.1:80491708-80491727 MS.gene48483:CDS 35.0%
TTGTAGAATCAGCATCAGAT+TGG + chr3.1:80493461-80493480 None:intergenic 35.0%
TTGTGGAATTCACTTCACAT+GGG - chr3.1:80492563-80492582 MS.gene48483:intron 35.0%
TTGTTCTCTACCTCATACTT+AGG - chr3.1:80493905-80493924 MS.gene48483:intron 35.0%
TTTGTGGAATTCACTTCACA+TGG - chr3.1:80492562-80492581 MS.gene48483:intron 35.0%
! AAAGTTTTGCAGACTTATGC+TGG - chr3.1:80492595-80492614 MS.gene48483:intron 35.0%
! AAGTTTTGCAGACTTATGCT+GGG - chr3.1:80492596-80492615 MS.gene48483:intron 35.0%
! ACTTTAGCAGCTTTAACTGA+AGG + chr3.1:80492787-80492806 None:intergenic 35.0%
! AGATTCGGTTATGCTTCATT+TGG - chr3.1:80491637-80491656 MS.gene48483:intron 35.0%
! CTTTAGCAGCTTTAACTGAA+GGG + chr3.1:80492786-80492805 None:intergenic 35.0%
! GATTCGGTTATGCTTCATTT+GGG - chr3.1:80491638-80491657 MS.gene48483:intron 35.0%
!! CATTTGGGTTTCGTGTTTTT+GGG - chr3.1:80491653-80491672 MS.gene48483:intron 35.0%
!! TCAGCATTATTTCTGCAGAT+AGG + chr3.1:80493796-80493815 None:intergenic 35.0%
!! TCATTTGGGTTTCGTGTTTT+TGG - chr3.1:80491652-80491671 MS.gene48483:intron 35.0%
!!! GTTGTTTTTGATGCCAATAC+CGG - chr3.1:80492805-80492824 MS.gene48483:CDS 35.0%
AAATCCTCTGGCTATCAACA+AGG - chr3.1:80492952-80492971 MS.gene48483:CDS 40.0%
AACGCAGAAGCTTAATGTCT+CGG + chr3.1:80492246-80492265 None:intergenic 40.0%
AAGCAGACTTGTCAAACAGA+GGG - chr3.1:80492532-80492551 MS.gene48483:intron 40.0%
AATGTATGGGCCTAAGTATG+AGG + chr3.1:80493918-80493937 None:intergenic 40.0%
AGAGACTATCTCACCATACT+GGG + chr3.1:80493626-80493645 None:intergenic 40.0%
AGCAAACAGTTCGTCTTTCT+TGG - chr3.1:80493854-80493873 MS.gene48483:intron 40.0%
ATACCTGTTGGAAAAGGATG+TGG - chr3.1:80493652-80493671 MS.gene48483:intron 40.0%
CAAACAGTTCGTCTTTCTTG+GGG - chr3.1:80493856-80493875 MS.gene48483:intron 40.0%
CGAGATTGAAAACTGAAACG+AGG + chr3.1:80493288-80493307 None:intergenic 40.0%
CTATAACTACATACACCCTC+CGG + chr3.1:80491902-80491921 None:intergenic 40.0%
CTGGAACATCTCTAGTAGTT+GGG - chr3.1:80493382-80493401 MS.gene48483:intron 40.0%
GCAAACAGTTCGTCTTTCTT+GGG - chr3.1:80493855-80493874 MS.gene48483:intron 40.0%
GTCTCTGTTAAGATACCTGT+TGG - chr3.1:80493640-80493659 MS.gene48483:intron 40.0%
TAAGCAGACTTGTCAAACAG+AGG - chr3.1:80492531-80492550 MS.gene48483:intron 40.0%
TATACAGGAGGCCAATCAAA+CGG - chr3.1:80493426-80493445 MS.gene48483:intron 40.0%
TCAATAGAGACCTTGTAGGA+TGG + chr3.1:80493948-80493967 None:intergenic 40.0%
TGAAAACTGCATGGAAAGTC+TGG - chr3.1:80493363-80493382 MS.gene48483:intron 40.0%
TGATAAGCCATGTAATGGTG+TGG + chr3.1:80491314-80491333 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+GGG - chr3.1:80491884-80491903 MS.gene48483:intron 40.0%
TTAGTGGGGTGTATTGGAAT+GGG - chr3.1:80492148-80492167 MS.gene48483:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr3.1:80491883-80491902 MS.gene48483:intron 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr3.1:80491902-80491883 None:intergenic 40.0%
! AAACCACATCCTTTTCCAAC+AGG + chr3.1:80493658-80493677 None:intergenic 40.0%
! AGACTTTCCATGCAGTTTTC+AGG + chr3.1:80493364-80493383 None:intergenic 40.0%
! ATGGTGTTTTCTGTTCTAGC+AGG - chr3.1:80492899-80492918 MS.gene48483:CDS 40.0%
! CTTTTCACCTGAAAACTGCA+TGG - chr3.1:80493354-80493373 MS.gene48483:intron 40.0%
! GATTCTCATTTGGTTCGAGA+TGG - chr3.1:80492445-80492464 MS.gene48483:intron 40.0%
! GTTTAAGGTTCAGTTGTTGC+AGG - chr3.1:80492035-80492054 MS.gene48483:intron 40.0%
! TAAGCATGTGTTTGGAACAG+CGG + chr3.1:80492310-80492329 None:intergenic 40.0%
! TCTGGAACATCTCTAGTAGT+TGG - chr3.1:80493381-80493400 MS.gene48483:intron 40.0%
! TGGTGTTTTCTGTTCTAGCA+GGG - chr3.1:80492900-80492919 MS.gene48483:CDS 40.0%
! TGGTTGTCTAAGATCTTCAG+CGG + chr3.1:80493599-80493618 None:intergenic 40.0%
! TTGTTATTACTCTGCTCCGT+TGG + chr3.1:80491146-80491165 None:intergenic 40.0%
!! GAAGCATTGCAGAAGCTAAA+TGG - chr3.1:80493817-80493836 MS.gene48483:intron 40.0%
AAGCTTAATGTCTCGGGAAG+CGG + chr3.1:80492239-80492258 None:intergenic 45.0%
ACCATAACCCTTTGAACGTC+CGG + chr3.1:80492827-80492846 None:intergenic 45.0%
ACGCAGAAGCTTAATGTCTC+GGG + chr3.1:80492245-80492264 None:intergenic 45.0%
ACTGTAAGTGAATGCGGTGA+AGG + chr3.1:80491197-80491216 None:intergenic 45.0%
ACTTGTCAAACAGAGGGGTA+TGG - chr3.1:80492538-80492557 MS.gene48483:intron 45.0%
AGAATCAGCATCAGATTGGC+TGG + chr3.1:80493457-80493476 None:intergenic 45.0%
AGACAACCATTCTCCCAGTA+TGG - chr3.1:80493610-80493629 MS.gene48483:intron 45.0%
AGCAGACTTGTCAAACAGAG+GGG - chr3.1:80492533-80492552 MS.gene48483:intron 45.0%
AGGATGGCCACACAAATGTA+TGG + chr3.1:80493932-80493951 None:intergenic 45.0%
ATCTCACCATACTGGGAGAA+TGG + chr3.1:80493619-80493638 None:intergenic 45.0%
ATGCTCTGGTCATAAGGATG+CGG + chr3.1:80494398-80494417 None:intergenic 45.0%
ATTTGTGTGGCCATCCTACA+AGG - chr3.1:80493935-80493954 MS.gene48483:intron 45.0%
CAAAGGGTTATGGTTTCGTC+AGG - chr3.1:80492833-80492852 MS.gene48483:CDS 45.0%
CAGAGACTATCTCACCATAC+TGG + chr3.1:80493627-80493646 None:intergenic 45.0%
CGAAAAGGTTGCTCTGTGTT+AGG + chr3.1:80492631-80492650 None:intergenic 45.0%
CGGAGCAGAGTAATAACAAC+AGG - chr3.1:80491147-80491166 MS.gene48483:CDS 45.0%
CTCTGGCTATCAACAAGGTA+TGG - chr3.1:80492957-80492976 MS.gene48483:CDS 45.0%
CTTAGGCCCATACATTTGTG+TGG - chr3.1:80493922-80493941 MS.gene48483:intron 45.0%
CTTAGTGGGGTGTATTGGAA+TGG - chr3.1:80492147-80492166 MS.gene48483:intron 45.0%
GAAAGCTTAGTGGGGTGTAT+TGG - chr3.1:80492142-80492161 MS.gene48483:intron 45.0%
GCACTTCTGACATGGGTTTA+AGG - chr3.1:80492020-80492039 MS.gene48483:intron 45.0%
GCATACATGCTCTGGTCATA+AGG + chr3.1:80494404-80494423 None:intergenic 45.0%
GGACCTGCTTATGATGGTTA+TGG - chr3.1:80494362-80494381 MS.gene48483:CDS 45.0%
GGATGGCCACACAAATGTAT+GGG + chr3.1:80493931-80493950 None:intergenic 45.0%
GGTGGAACTGTAAGTGAATG+CGG + chr3.1:80491203-80491222 None:intergenic 45.0%
GGTTATGGTTTCGTCAGGTT+TGG - chr3.1:80492838-80492857 MS.gene48483:CDS 45.0%
TAAGCCATGTAATGGTGTGG+TGG + chr3.1:80491311-80491330 None:intergenic 45.0%
TAAGGTTCAGTTGTTGCAGG+TGG - chr3.1:80492038-80492057 MS.gene48483:intron 45.0%
TGAGGCGGTAACATATGATG+CGG + chr3.1:80491275-80491294 None:intergenic 45.0%
TGATAGCCAGAGGATTTCCT+AGG + chr3.1:80492949-80492968 None:intergenic 45.0%
TGCTAAATGTCGCCCAGTTA+AGG + chr3.1:80492654-80492673 None:intergenic 45.0%
TTCGTCAGGTTTGGAGATGA+TGG - chr3.1:80492847-80492866 MS.gene48483:CDS 45.0%
! AACAGAGGGGTATGGATTTG+TGG - chr3.1:80492546-80492565 MS.gene48483:intron 45.0%
! AGCAACCTTTTCGCCTTAAC+TGG - chr3.1:80492638-80492657 MS.gene48483:CDS 45.0%
! CGGTTCTGATTCGAAACCAA+CGG - chr3.1:80491127-80491146 MS.gene48483:CDS 45.0%
! GAATCAGCATCAGATTGGCT+GGG + chr3.1:80493456-80493475 None:intergenic 45.0%
! GCAACCTTTTCGCCTTAACT+GGG - chr3.1:80492639-80492658 MS.gene48483:CDS 45.0%
! GGTTTGGAGATGATGGTGAA+AGG - chr3.1:80492854-80492873 MS.gene48483:CDS 45.0%
! TGTTGGTGTCCTCCATACAT+GGG + chr3.1:80494437-80494456 None:intergenic 45.0%
!! CATACCTTGTTGATAGCCAG+AGG + chr3.1:80492959-80492978 None:intergenic 45.0%
AACGGGCCAGCATATTATGG+GGG - chr3.1:80494341-80494360 MS.gene48483:CDS 50.0%
AACTGGGCGACATTTAGCAC+AGG - chr3.1:80492655-80492674 MS.gene48483:CDS 50.0%
AAGCAGCAGCATACATGCTC+TGG + chr3.1:80494412-80494431 None:intergenic 50.0%
ACCCTTTGAACGTCCGGTAT+TGG + chr3.1:80492821-80492840 None:intergenic 50.0%
ACCGGACGTTCAAAGGGTTA+TGG - chr3.1:80492823-80492842 MS.gene48483:CDS 50.0%
AGATCTTCAGCGGTAGCACT+AGG + chr3.1:80493589-80493608 None:intergenic 50.0%
ATAATATGCTGGCCCGTTCC+AGG + chr3.1:80494339-80494358 None:intergenic 50.0%
CCTGCTTATTTGCCGGATTG+CGG + chr3.1:80493882-80493901 None:intergenic 50.0%
CTAGATCCATGATGCACGTG+AGG + chr3.1:80491356-80491375 None:intergenic 50.0%
GAACGGGCCAGCATATTATG+GGG - chr3.1:80494340-80494359 MS.gene48483:CDS 50.0%
GATATTGCATCGGAACCCAC+TGG + chr3.1:80491232-80491251 None:intergenic 50.0%
GATCTTCAGCGGTAGCACTA+GGG + chr3.1:80493588-80493607 None:intergenic 50.0%
GCAACTCCTAGGAAATCCTC+TGG - chr3.1:80492940-80492959 MS.gene48483:CDS 50.0%
GCCAATACCGGACGTTCAAA+GGG - chr3.1:80492817-80492836 MS.gene48483:CDS 50.0%
GGAACGGGCCAGCATATTAT+GGG - chr3.1:80494339-80494358 MS.gene48483:CDS 50.0%
GGTTCCGATGCAATATCCTC+CGG - chr3.1:80491235-80491254 MS.gene48483:CDS 50.0%
GTAAGTGAATGCGGTGAAGG+TGG + chr3.1:80491194-80491213 None:intergenic 50.0%
TAATATGCTGGCCCGTTCCA+GGG + chr3.1:80494338-80494357 None:intergenic 50.0%
TATGGGGGACCTGCTTATGA+TGG - chr3.1:80494356-80494375 MS.gene48483:CDS 50.0%
TCTAGCAGGGCAATGCGAAT+TGG - chr3.1:80492913-80492932 MS.gene48483:CDS 50.0%
TGCCAATACCGGACGTTCAA+AGG - chr3.1:80492816-80492835 MS.gene48483:CDS 50.0%
TGGAACGGGCCAGCATATTA+TGG - chr3.1:80494338-80494357 MS.gene48483:CDS 50.0%
TGTCTCGGGAAGCGGAAAAA+TGG + chr3.1:80492231-80492250 None:intergenic 50.0%
! CGGTGATTTGCACCATTGGA+TGG - chr3.1:80491403-80491422 MS.gene48483:intron 50.0%
! CTGTTGGTGTCCTCCATACA+TGG + chr3.1:80494438-80494457 None:intergenic 50.0%
! GTTGGTGTCCTCCATACATG+GGG + chr3.1:80494436-80494455 None:intergenic 50.0%
!! GAATTGGTGCTGCAACTCCT+AGG - chr3.1:80492929-80492948 MS.gene48483:CDS 50.0%
AAAAGGGAAAACCTCGCCGG+TGG + chr3.1:80491459-80491478 None:intergenic 55.0%
AATTCGGCAGTCCCTGGAAC+GGG - chr3.1:80494324-80494343 MS.gene48483:CDS 55.0%
AGTGAATGCGGTGAAGGTGG+TGG + chr3.1:80491191-80491210 None:intergenic 55.0%
CCGCAATCCGGCAAATAAGC+AGG - chr3.1:80493879-80493898 MS.gene48483:intron 55.0%
GAATTCGGCAGTCCCTGGAA+CGG - chr3.1:80494323-80494342 MS.gene48483:CDS 55.0%
GATCTGAATTCGGCAGTCCC+TGG - chr3.1:80494318-80494337 MS.gene48483:CDS 55.0%
GCAGGTCCCCCATAATATGC+TGG + chr3.1:80494350-80494369 None:intergenic 55.0%
GCCATGTAATGGTGTGGTGG+CGG + chr3.1:80491308-80491327 None:intergenic 55.0%
GCTGCTTACCCCATGTATGG+AGG - chr3.1:80494425-80494444 MS.gene48483:CDS 55.0%
GGGAAAAGGGAAAACCTCGC+CGG + chr3.1:80491462-80491481 None:intergenic 55.0%
GTCGCCCAGTTAAGGCGAAA+AGG + chr3.1:80492646-80492665 None:intergenic 55.0%
TCCGATGCAATATCCTCCGG+CGG - chr3.1:80491238-80491257 MS.gene48483:CDS 55.0%
TCTTTCTTGGGGCCGCAATC+CGG - chr3.1:80493867-80493886 MS.gene48483:intron 55.0%
! CACCGTTGTTTCGCTTCCAC+CGG - chr3.1:80491440-80491459 MS.gene48483:intron 55.0%
! GCTGCTGCTTACCCCATGTA+TGG - chr3.1:80494422-80494441 MS.gene48483:CDS 55.0%
! GGGTCGGTGATTTGCACCAT+TGG - chr3.1:80491399-80491418 MS.gene48483:intron 55.0%
!! ATGTAATGGTGTGGTGGCGG+CGG + chr3.1:80491305-80491324 None:intergenic 55.0%
!!! GGTGGCGGAGTTTTCTGTTG+CGG + chr3.1:80491173-80491192 None:intergenic 55.0%
AGTTCCACCACCGCACCAGT+GGG - chr3.1:80491214-80491233 MS.gene48483:CDS 60.0%
CACGTGCCTCACGTGCATCA+TGG - chr3.1:80491347-80491366 MS.gene48483:intron 60.0%
CAGCGGTAGCACTAGGGTCA+AGG + chr3.1:80493582-80493601 None:intergenic 60.0%
CATGATGCACGTGAGGCACG+TGG + chr3.1:80491349-80491368 None:intergenic 60.0%
CGCCGGTGGAAGCGAAACAA+CGG + chr3.1:80491445-80491464 None:intergenic 60.0%
CGGTGGAAGCGAAACAACGG+TGG + chr3.1:80491442-80491461 None:intergenic 60.0%
GCAATATCCTCCGGCGGCAA+TGG - chr3.1:80491244-80491263 MS.gene48483:CDS 60.0%
GCCGCCACCACACCATTACA+TGG - chr3.1:80491304-80491323 MS.gene48483:intron 60.0%
GCCGCCGGAGGATATTGCAT+CGG + chr3.1:80491242-80491261 None:intergenic 60.0%
GGCGGCGGATAATGTTGAGG+CGG + chr3.1:80491290-80491309 None:intergenic 60.0%
TGCATCGGAACCCACTGGTG+CGG + chr3.1:80491227-80491246 None:intergenic 60.0%
TGCGGCATTACCATTGCCGC+CGG + chr3.1:80491257-80491276 None:intergenic 60.0%
TTGTTTCGCTTCCACCGGCG+AGG - chr3.1:80491445-80491464 MS.gene48483:intron 60.0%
! GAATGCGGTGAAGGTGGTGG+CGG + chr3.1:80491188-80491207 None:intergenic 60.0%
! GGTGGCGGCGGATAATGTTG+AGG + chr3.1:80491293-80491312 None:intergenic 60.0%
!! GGCTGGGTGTGCCGTTTGAT+TGG + chr3.1:80493440-80493459 None:intergenic 60.0%
ATCGGAACCCACTGGTGCGG+TGG + chr3.1:80491224-80491243 None:intergenic 65.0%
CAGTTCCACCACCGCACCAG+TGG - chr3.1:80491213-80491232 MS.gene48483:CDS 65.0%
GGCATTACCATTGCCGCCGG+AGG + chr3.1:80491254-80491273 None:intergenic 65.0%
GGAACCCACTGGTGCGGTGG+TGG + chr3.1:80491221-80491240 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 80491113 80494469 80491113 ID=MS.gene48483
chr3.1 mRNA 80491113 80494469 80491113 ID=MS.gene48483.t1;Parent=MS.gene48483
chr3.1 exon 80494182 80494469 80494182 ID=MS.gene48483.t1.exon1;Parent=MS.gene48483.t1
chr3.1 CDS 80494182 80494469 80494182 ID=cds.MS.gene48483.t1;Parent=MS.gene48483.t1
chr3.1 exon 80492609 80493077 80492609 ID=MS.gene48483.t1.exon2;Parent=MS.gene48483.t1
chr3.1 CDS 80492609 80493077 80492609 ID=cds.MS.gene48483.t1;Parent=MS.gene48483.t1
chr3.1 exon 80492094 80492149 80492094 ID=MS.gene48483.t1.exon3;Parent=MS.gene48483.t1
chr3.1 CDS 80492094 80492149 80492094 ID=cds.MS.gene48483.t1;Parent=MS.gene48483.t1
chr3.1 exon 80491887 80492017 80491887 ID=MS.gene48483.t1.exon4;Parent=MS.gene48483.t1
chr3.1 CDS 80491887 80492017 80491887 ID=cds.MS.gene48483.t1;Parent=MS.gene48483.t1
chr3.1 exon 80491682 80491778 80491682 ID=MS.gene48483.t1.exon5;Parent=MS.gene48483.t1
chr3.1 CDS 80491682 80491778 80491682 ID=cds.MS.gene48483.t1;Parent=MS.gene48483.t1
chr3.1 exon 80491113 80491268 80491113 ID=MS.gene48483.t1.exon6;Parent=MS.gene48483.t1
chr3.1 CDS 80491113 80491268 80491113 ID=cds.MS.gene48483.t1;Parent=MS.gene48483.t1
Gene Sequence

>MS.gene48483

ATGCAATCAACAAACGGTTCTGATTCGAAACCAACGGAGCAGAGTAATAACAACAGGCCGCAACAGAAAACTCCGCCACCACCTTCACCGCATTCACTTACAGTTCCACCACCGCACCAGTGGGTTCCGATGCAATATCCTCCGGCGGCAATGGTAATGCCGCATCATATGTTACCGCCTCAACATTATCCGCCGCCACCACACCATTACATGGCTTATCATCATTATCTTCACCACGTGCCTCACGTGCATCATGGATCTAGTGCTGCTGATAATAAAACTCTCTGGGTCGGTGATTTGCACCATTGGATGGATGAAAATTATCTCCACCGTTGTTTCGCTTCCACCGGCGAGGTTTTCCCTTTTCCCTTTTTCTTTCTTTCTTTTTGTTTTTTCGTTACTGATTTTATTTATGAACATTAAATCGTATTTTTATGTTTTGTTACTTTTATGTTTTCTGATATCAATGTTCTTTGATTGTAAGTTTATGGAAAATGGATATTACAGTTTGTCGTTTCTACTTAAGATTCGGTTATGCTTCATTTGGGTTTCGTGTTTTTGGGTTTTTTTGTTATTAAGTAAATTTTTTGGGTTTTGTGTTAACCCTCAGAAAAAAGGGAACTTTTTTTTTTTTTTTACTAATTGTGTACATCCTCCGGTCACTATTATAAGCAAAAATCAACATTCTAGGTTCATTCATTAAATGATGTATGTGGTCTATAATAAAGACTATATACATCATTAATTGAATGAATCTCAAATGTGAATTTTTGCTTATAATAGTGACCGGAGGGTGTATGTAGTTATAGTACAATTGTTAAATCAAGTTTAAAGATTAGTAATTGAAAAGATACTAAAGCCATGTGCATTAATAGTTTTTTGGATTAAAGAGAAGATCGAAATGTTTGCACTTCTGACATGGGTTTAAGGTTCAGTTGTTGCAGGTGGCTAGATTTAACCCAAAAAGTGACATTATTGCAATCTTTTTATGCCACATTTGTTTGGTGAAAAAATAGTACTATGAATTTTGAAAGCTTAGTGGGGTGTATTGGAATGGGATTTTAAAGCATTTTAAAAGAGTTTTTTAAGCATGTATTTGAAACTGGGTTTGCAAACCATTTTTCCGCTTCCCGAGACATTAAGCTTCTGCGTTGACGTATTAACCGATTTCTTATGGTGATTTCTCAAAATATGCCGCTGTTCCAAACACATGCTTATGATAGAAAATTACCGTAATATTCAATCGAGATTATTATTTAATCCAAATAAATGTTGTGGTATTTATTTAGGAGAATCAAGTTTTCTCATTTTGGTAGTAAAAATGGTTGTTGAGATTCTCATTTGGTTCGAGATGGATAAACTTTTGCATCTAATTGACATGCATTTTTGCAGATTTTCTCCATTAAGGTTATTCGCAATAAGCAGACTTGTCAAACAGAGGGGTATGGATTTGTGGAATTCACTTCACATGGGACAGCTGAGAAAGTTTTGCAGACTTATGCTGGGATGTTGATGCCTAACACAGAGCAACCTTTTCGCCTTAACTGGGCGACATTTAGCACAGGAGATCACAAACGCTCAGATAATGTCCCTGATCTTTCTATTTTTGTAGGAGATTTAGCTGCAGATGTTACAGATACCATGTTACTTGAAACTTTTTCTGATAAATACCCTTCAGTTAAAGCTGCTAAAGTTGTTTTTGATGCCAATACCGGACGTTCAAAGGGTTATGGTTTCGTCAGGTTTGGAGATGATGGTGAAAGGTCTAAAGCTTTGAATGAAATGAATGGTGTTTTCTGTTCTAGCAGGGCAATGCGAATTGGTGCTGCAACTCCTAGGAAATCCTCTGGCTATCAACAAGGTATGGCAACTTATATAGCGCCTCATATCTGAAATTTCACTCTTCATGTAGTAGAAAAGAAATGTGGTCTACATTCAATTCGGAGACAAACAATTTTAGATATTTTTTTATGCTCAAATTGTATTTGTTCTGCTTTGTATTTTTGTCGAGAAACTTATGGAATTGCATTTTTATTGCTCATATAAATGTATGCTTTGTTTTATGGTATAATTGGCGATATCAATTTTGTTAATAAAATGTTATTTGTTTCTAATTAGCAGACAAGTAGTAAATTCATAGAATTTCCTGAATCCATTCACTTGTTTATTTCTCCTCGTTTCAGTTTTCAATCTCGTGTAGTTATTTTTTTTAATGTCTCTCCATTTCAACATTTGCATATGCTTTTCACCTGAAAACTGCATGGAAAGTCTGGAACATCTCTAGTAGTTGGGTATTGATTTAGTTTGTTTCTTTATACAGGAGGCCAATCAAACGGCACACCCAGCCAATCTGATGCTGATTCTACAAATACAACTGTTAGTTTTTCAGCTTTTTATTAATTTTTTTTTAGAGAAGTTAATCTGTCACTAATTTTGTAAGGATGCATTATAGATATTTGTTGGAGGCCTTGACCCTAGTGCTACCGCTGAAGATCTTAGACAACCATTCTCCCAGTATGGTGAGATAGTCTCTGTTAAGATACCTGTTGGAAAAGGATGTGGTTTTGTACAATTTGCAAACAGGTAAAATTTATATCCAATTTTTACTCTGCATTTATTATGAGATAACAAGGTTTTTACTACACTCGCAATGTGTTCACTAATTAATCATTCTAAACAACCTATCTGCAGAAATAATGCTGAAGAAGCATTGCAGAAGCTAAATGGTACAACAGTTGGCAAGCAAACAGTTCGTCTTTCTTGGGGCCGCAATCCGGCAAATAAGCAGGTAATTGTTCTCTACCTCATACTTAGGCCCATACATTTGTGTGGCCATCCTACAAGGTCTCTATTGATCTATAGGGGCAAGTGTAATTTAATGACCAATAACTCACCTAGTAATACCATGAACTTCATGTAGGTTTTAGGAGAAACAAAAGGTCTATTTGTTCTCAGCTGCTAAATTTAATGAAGTGTAGTTTAATGCAGTCATTATATGATTTTTTTTTTGCTTAATTCGAATCAATCAAATTTTGTTCATGATTATTTTGTTCATTAATGAATTTTCACATTCAAATGAAGACTCTAAGAATAACTATTGATGAGTATAGCTCATTGAATACCAGATTGTCTCATTCTGTCTATCATTGATTGCATTTTGCAATTGTTCTATGTCAATTGACACATCTCTTTGCCTAGCAGTTTAGATCTGAATTCGGCAGTCCCTGGAACGGGCCAGCATATTATGGGGGACCTGCTTATGATGGTTATGGATATGCTATGCCGCATCCTTATGACCAGAGCATGTATGCTGCTGCTTACCCCATGTATGGAGGACACCAACAGCAAGTAAGCTAA
Protein sequence

>MS.gene48483.t1

MQSTNGSDSKPTEQSNNNRPQQKTPPPPSPHSLTVPPPHQWVPMQYPPAAMVMPHHMLPPQHYPPPPHHYMAYHHYLHHVPHVHHGSSAADNKTLWIFSIKVIRNKQTCQTEGYGFVEFTSHGTAEKVLQTYAGMLMPNTEQPFRLNWATFSTGDHKRSDNVPDLSIFVGDLAADVTDTMLLETFSDKYPSVKAAKVVFDANTGRSKGYGFVRFGDDGERSKALNEMNGVFCSSRAMRIGAATPRKSSGYQQGGQSNGTPSQSDADSTNTTIFVGGLDPSATAEDLRQPFSQYGEIVSVKIPVGKGCGFVQFANRNNAEEALQKLNGTTVGKQTVRLSWGRNPANKQFRSEFGSPWNGPAYYGGPAYDGYGYAMPHPYDQSMYAAAYPMYGGHQQQVS