AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene044004

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044004.t1 MTR_1g020080 99.291 282 2 0 1 282 123 404 0.0 582
MS.gene044004.t1 MTR_1g020080 27.136 199 115 6 64 246 93 277 3.40e-13 69.7
MS.gene044004.t1 MTR_3g101370 79.787 282 54 3 1 279 119 400 2.02e-161 455
MS.gene044004.t1 MTR_3g101370 27.513 189 115 6 61 240 86 261 2.11e-13 70.1
MS.gene044004.t1 MTR_4g086600 66.058 274 73 3 1 254 142 415 5.31e-126 366
MS.gene044004.t1 MTR_1g020080 96.111 180 7 0 1 180 123 302 6.54e-123 353
MS.gene044004.t1 MTR_1g020080 27.136 199 115 6 64 246 93 277 4.08e-13 68.6
MS.gene044004.t1 MTR_4g131970 58.824 289 101 4 1 277 100 382 3.70e-109 322
MS.gene044004.t1 MTR_2g022950 57.388 291 115 3 1 282 86 376 4.79e-107 316
MS.gene044004.t1 MTR_2g097960 50.993 302 119 3 1 273 41 342 7.00e-103 306
MS.gene044004.t1 MTR_3g101370 79.545 176 35 1 1 175 119 294 4.03e-96 285
MS.gene044004.t1 MTR_3g101370 28.125 192 113 7 58 240 86 261 1.25e-13 70.1
MS.gene044004.t1 MTR_2g022950 63.057 157 56 1 1 155 86 242 3.20e-63 200
MS.gene044004.t1 MTR_2g022950 26.316 190 117 6 69 247 61 238 6.08e-13 67.8
MS.gene044004.t1 MTR_2g022950 63.057 157 56 1 1 155 86 242 8.16e-63 199
MS.gene044004.t1 MTR_2g022950 26.316 190 117 6 69 247 61 238 8.51e-13 67.4
MS.gene044004.t1 MTR_3g109090 31.579 266 153 6 1 246 70 326 5.49e-34 128
MS.gene044004.t1 MTR_3g109090 25.532 188 114 4 61 242 37 204 2.21e-12 67.0
MS.gene044004.t1 MTR_3g109090 31.579 266 153 6 1 246 86 342 6.20e-34 128
MS.gene044004.t1 MTR_3g109090 25.532 188 114 4 61 242 53 220 2.01e-12 67.4
MS.gene044004.t1 MTR_4g124940 31.698 265 153 9 1 246 72 327 1.33e-30 118
MS.gene044004.t1 MTR_4g124940 27.513 189 111 4 61 243 39 207 2.15e-14 73.2
MS.gene044004.t1 MTR_8g022050 30.597 268 153 10 1 246 88 344 4.08e-27 109
MS.gene044004.t1 MTR_8g022050 27.128 188 111 4 61 242 55 222 8.67e-15 74.3
MS.gene044004.t1 MTR_4g085540 29.389 262 168 7 1 253 148 401 2.07e-26 108
MS.gene044004.t1 MTR_4g085540 32.222 180 100 4 64 238 30 192 4.56e-20 90.1
MS.gene044004.t1 MTR_1g019150 28.958 259 174 6 1 253 146 400 3.37e-24 102
MS.gene044004.t1 MTR_1g019150 27.311 238 149 7 1 233 58 276 1.37e-17 82.8
MS.gene044004.t1 MTR_3g102040 26.374 273 191 6 1 267 138 406 6.94e-22 95.5
MS.gene044004.t1 MTR_3g102040 31.492 181 102 4 63 238 19 182 1.60e-19 88.6
MS.gene044004.t1 MTR_3g102040 31.064 235 138 8 1 230 50 265 1.14e-17 83.2
MS.gene044004.t1 MTR_1g097440 31.873 251 142 11 1 244 56 284 9.59e-22 95.1
MS.gene044004.t1 MTR_1g097440 22.581 248 183 5 12 253 154 398 3.64e-16 78.6
MS.gene044004.t1 MTR_1g097440 26.257 179 110 4 69 242 31 192 9.48e-13 68.6
MS.gene044004.t1 MTR_7g102730 26.538 260 157 8 1 250 36 271 1.81e-21 94.4
MS.gene044004.t1 MTR_7g102730 27.711 249 171 5 1 244 124 368 1.04e-20 92.0
MS.gene044004.t1 MTR_7g102730 26.519 181 105 4 66 238 8 168 6.52e-15 75.1
MS.gene044004.t1 MTR_1g061870 24.903 257 165 8 1 250 41 276 5.24e-18 84.0
MS.gene044004.t1 MTR_1g061870 27.429 175 105 4 69 238 16 173 6.00e-14 72.0
MS.gene044004.t1 MTR_1g061870 27.049 244 151 8 1 232 129 357 1.09e-12 68.2
MS.gene044004.t1 MTR_6g040190 29.050 179 105 4 69 242 26 187 1.38e-17 82.8
MS.gene044004.t1 MTR_6g040190 23.552 259 184 7 1 253 139 389 2.45e-14 73.2
MS.gene044004.t1 MTR_6g040070 25.097 259 183 6 1 253 41 294 1.83e-17 82.8
MS.gene044004.t1 MTR_6g040070 32.738 168 93 5 69 231 16 168 1.47e-15 77.0
MS.gene044004.t1 MTR_4g104470 27.225 191 122 3 67 251 121 300 1.71e-16 78.2
MS.gene044004.t1 MTR_4g104470 30.818 159 86 5 5 150 152 299 8.58e-14 70.5
MS.gene044004.t1 MTR_1g064230 26.761 213 139 4 54 251 70 280 9.57e-15 73.2
MS.gene044004.t1 MTR_1g080150 36.782 87 54 1 67 153 131 216 1.27e-14 72.0
MS.gene044004.t1 MTR_3g065190 28.108 185 124 4 1 182 51 229 2.41e-14 72.8
MS.gene044004.t1 MTR_3g065190 24.000 200 123 5 58 250 15 192 1.59e-12 67.4
MS.gene044004.t1 MTR_5g097580 29.885 174 107 5 70 233 8 176 7.95e-14 71.6
MS.gene044004.t1 MTR_5g097580 29.885 174 107 5 70 233 8 176 8.71e-14 71.2
MS.gene044004.t1 MTR_3g075500 30.435 161 89 4 5 152 139 289 9.24e-14 70.5
MS.gene044004.t1 MTR_3g075500 26.519 181 119 3 67 241 108 280 6.46e-13 67.8
MS.gene044004.t1 MTR_3g079200 38.095 84 51 1 162 244 217 300 1.33e-13 70.9
MS.gene044004.t1 MTR_8g092550 27.778 180 118 6 69 241 203 377 1.91e-13 70.5
MS.gene044004.t1 MTR_1g045510 30.667 150 95 4 4 150 142 285 2.08e-13 69.3
MS.gene044004.t1 MTR_7g118120 22.656 256 169 6 1 250 130 362 2.58e-13 70.1
MS.gene044004.t1 MTR_2g026180 30.682 176 105 6 70 233 6 176 2.61e-13 70.1
MS.gene044004.t1 MTR_3g055990 28.736 174 109 5 70 233 8 176 3.75e-13 69.7
MS.gene044004.t1 MTR_3g055990 28.736 174 109 5 70 233 8 176 4.71e-13 69.3
MS.gene044004.t1 MTR_1g056520 29.586 169 107 5 1 160 134 299 1.60e-12 67.0
MS.gene044004.t1 MTR_3g095230 26.630 184 116 7 69 241 216 391 3.88e-12 66.6
MS.gene044004.t1 MTR_3g095230 26.630 184 116 7 69 241 216 391 4.71e-12 66.2
MS.gene044004.t1 MTR_7g094660 25.134 187 124 4 55 233 628 806 7.92e-12 65.9
MS.gene044004.t1 MTR_3g027140 26.257 179 116 5 70 240 62 232 1.14e-11 63.5
MS.gene044004.t1 MTR_4g068120 27.273 209 114 5 69 271 28 204 1.51e-11 65.1
MS.gene044004.t1 MTR_3g103420 26.257 179 118 4 70 237 12 187 1.88e-11 64.3
MS.gene044004.t1 MTR_3g075500 26.471 170 111 3 67 230 108 269 4.07e-11 62.4
MS.gene044004.t1 MTR_4g122650 25.446 224 138 7 70 282 90 295 7.23e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene044004.t1 AT1G47500 71.329 286 76 4 1 282 131 414 4.16e-142 407
MS.gene044004.t1 AT1G47500 27.273 187 114 6 64 240 99 273 1.15e-13 70.9
MS.gene044004.t1 AT1G47490 70.922 282 78 3 1 278 129 410 1.92e-141 405
MS.gene044004.t1 AT3G19130 64.388 278 82 5 1 262 134 410 7.37e-122 355
MS.gene044004.t1 AT1G49600 64.835 273 84 5 1 262 145 416 3.89e-120 351
MS.gene044004.t1 AT1G49600 30.208 192 110 7 58 240 111 287 1.37e-14 73.9
MS.gene044004.t1 AT1G49600 66.016 256 77 3 1 246 145 400 3.56e-117 344
MS.gene044004.t1 AT1G49600 30.208 192 110 7 58 240 111 287 3.40e-15 75.5
MS.gene044004.t1 AT1G49600 66.016 256 77 3 1 246 145 400 3.56e-117 344
MS.gene044004.t1 AT1G49600 30.208 192 110 7 58 240 111 287 3.40e-15 75.5
MS.gene044004.t1 AT1G11650 61.111 252 95 3 1 250 88 338 5.96e-103 306
MS.gene044004.t1 AT4G27000 58.498 253 105 0 2 254 107 359 1.85e-102 305
MS.gene044004.t1 AT5G54900 55.439 285 121 5 2 282 87 369 9.75e-100 297
MS.gene044004.t1 AT5G19350 56.202 258 100 2 1 246 50 306 3.30e-95 287
MS.gene044004.t1 AT5G19350 54.962 262 101 2 1 246 50 310 1.41e-94 285
MS.gene044004.t1 AT1G11650 60.909 220 83 3 1 218 88 306 8.57e-89 266
MS.gene044004.t1 AT1G11650 27.473 182 107 6 69 240 63 229 4.27e-12 65.9
MS.gene044004.t1 AT1G47490 68.889 180 53 2 1 177 129 308 5.57e-83 252
MS.gene044004.t1 AT1G47490 28.022 182 109 6 69 240 102 271 7.73e-15 73.6
MS.gene044004.t1 AT1G47500 69.274 179 52 2 1 176 131 309 9.93e-82 249
MS.gene044004.t1 AT1G47500 27.473 182 110 6 69 240 104 273 1.11e-13 70.5
MS.gene044004.t1 AT1G54080 34.586 266 145 11 1 246 89 345 1.40e-33 127
MS.gene044004.t1 AT1G54080 26.984 189 112 4 61 243 56 224 6.69e-17 80.5
MS.gene044004.t1 AT1G54080 34.686 271 142 12 1 246 89 349 1.13e-32 124
MS.gene044004.t1 AT1G54080 27.461 193 110 4 61 243 56 228 1.74e-16 79.3
MS.gene044004.t1 AT3G14100 32.331 266 151 9 1 246 85 341 1.31e-31 121
MS.gene044004.t1 AT3G14100 27.513 189 111 4 61 243 52 220 1.54e-17 82.4
MS.gene044004.t1 AT1G17370 32.075 265 150 10 1 246 80 333 2.06e-30 118
MS.gene044004.t1 AT1G17370 27.660 188 110 4 61 242 47 214 4.86e-16 77.8
MS.gene044004.t1 AT1G17370 31.716 268 150 10 1 246 80 336 5.57e-30 117
MS.gene044004.t1 AT1G17370 27.660 188 110 4 61 242 47 214 5.30e-16 77.8
MS.gene044004.t1 AT2G23350 28.788 264 167 9 1 253 160 413 4.32e-24 102
MS.gene044004.t1 AT2G23350 31.092 238 134 9 1 230 72 287 4.72e-19 87.4
MS.gene044004.t1 AT2G23350 30.286 175 100 3 69 238 47 204 1.68e-15 76.6
MS.gene044004.t1 AT4G34110 28.295 258 176 6 1 253 150 403 4.82e-24 102
MS.gene044004.t1 AT4G34110 30.000 240 134 9 1 230 62 277 1.44e-21 94.7
MS.gene044004.t1 AT4G34110 32.000 175 97 4 69 238 37 194 1.54e-19 88.6
MS.gene044004.t1 AT1G34140 25.347 288 188 8 1 282 57 323 6.17e-23 97.8
MS.gene044004.t1 AT1G71770 27.961 304 184 12 1 280 172 464 7.39e-23 98.6
MS.gene044004.t1 AT1G71770 30.043 233 133 8 15 238 98 309 1.09e-21 95.1
MS.gene044004.t1 AT1G71770 31.429 175 97 5 69 238 60 216 2.55e-17 82.4
MS.gene044004.t1 AT1G71770 27.961 304 184 12 1 280 172 464 7.39e-23 98.6
MS.gene044004.t1 AT1G71770 30.043 233 133 8 15 238 98 309 1.09e-21 95.1
MS.gene044004.t1 AT1G71770 31.429 175 97 5 69 238 60 216 2.55e-17 82.4
MS.gene044004.t1 AT1G49760 27.799 259 176 6 1 253 159 412 3.83e-22 96.3
MS.gene044004.t1 AT1G49760 32.000 175 97 4 69 238 46 203 3.47e-18 84.7
MS.gene044004.t1 AT1G49760 27.799 259 176 6 1 253 159 412 3.83e-22 96.3
MS.gene044004.t1 AT1G49760 32.000 175 97 4 69 238 46 203 3.47e-18 84.7
MS.gene044004.t1 AT1G22760 27.490 251 171 6 1 244 162 408 1.52e-21 94.7
MS.gene044004.t1 AT1G22760 27.755 245 150 10 1 238 75 299 7.79e-18 83.6
MS.gene044004.t1 AT2G37220 29.703 202 125 5 66 251 89 289 1.63e-18 83.6
MS.gene044004.t1 AT3G16380 27.642 246 150 8 1 238 47 272 1.96e-18 85.5
MS.gene044004.t1 AT3G16380 28.340 247 159 8 1 238 138 375 2.07e-18 85.1
MS.gene044004.t1 AT3G16380 28.994 169 102 3 69 233 22 176 5.54e-13 69.3
MS.gene044004.t1 AT2G36660 23.529 255 169 7 1 250 50 283 6.71e-18 84.0
MS.gene044004.t1 AT2G36660 24.723 271 171 7 1 255 138 391 1.30e-15 77.0
MS.gene044004.t1 AT2G36660 26.286 175 107 4 69 238 25 182 2.32e-14 73.6
MS.gene044004.t1 AT3G04500 33.945 109 66 2 67 175 136 238 1.66e-15 74.7
MS.gene044004.t1 AT4G14300 32.022 178 106 6 70 237 8 180 1.58e-14 73.6
MS.gene044004.t1 AT4G14300 32.022 178 106 6 70 237 8 180 1.58e-14 73.6
MS.gene044004.t1 AT5G47620 28.718 195 117 7 70 252 8 192 3.99e-14 72.4
MS.gene044004.t1 AT5G47620 28.718 195 117 7 70 252 8 192 3.99e-14 72.4
MS.gene044004.t1 AT5G47620 28.718 195 117 7 70 252 8 192 3.99e-14 72.4
MS.gene044004.t1 AT1G07360 39.506 81 48 1 165 244 220 300 4.35e-14 72.4
MS.gene044004.t1 AT3G13224 27.041 196 117 6 55 243 10 186 6.35e-14 70.1
MS.gene044004.t1 AT3G07810 31.250 176 104 6 70 233 8 178 6.92e-14 71.6
MS.gene044004.t1 AT3G07810 31.250 176 104 6 70 233 8 178 7.46e-14 71.6
MS.gene044004.t1 AT2G29580 38.272 81 49 1 165 244 220 300 1.62e-13 70.9
MS.gene044004.t1 AT3G13224 27.119 177 106 5 63 233 14 173 7.12e-13 68.6
MS.gene044004.t1 AT3G13224 28.667 150 103 2 1 150 45 190 2.26e-12 67.0
MS.gene044004.t1 AT3G13224 27.647 170 100 5 70 233 12 164 7.94e-13 68.2
MS.gene044004.t1 AT3G13224 28.667 150 103 2 1 150 36 181 1.83e-12 67.0
MS.gene044004.t1 AT4G00830 22.182 275 180 7 1 267 142 390 1.28e-12 68.2
MS.gene044004.t1 AT4G00830 22.182 275 180 7 1 267 142 390 1.28e-12 68.2
MS.gene044004.t1 AT4G00830 22.182 275 180 7 1 267 142 390 1.28e-12 68.2
MS.gene044004.t1 AT2G18510 26.437 174 117 4 1 174 51 213 1.29e-12 67.8
MS.gene044004.t1 AT5G50250 25.424 177 115 3 70 240 115 280 1.31e-12 67.0
MS.gene044004.t1 AT5G47620 28.016 257 133 13 70 282 8 256 2.21e-12 67.4
MS.gene044004.t1 AT1G58470 28.977 176 102 6 70 226 8 179 2.80e-12 66.6
MS.gene044004.t1 AT1G58470 28.977 176 102 6 70 226 8 179 2.80e-12 66.6
MS.gene044004.t1 AT5G55550 27.841 176 112 5 70 233 8 180 3.15e-12 66.6
MS.gene044004.t1 AT5G55550 27.841 176 112 5 70 233 8 180 3.15e-12 66.6
MS.gene044004.t1 AT5G55550 27.841 176 112 5 70 233 8 180 3.15e-12 66.6
MS.gene044004.t1 AT5G55550 27.841 176 112 5 70 233 8 180 3.15e-12 66.6
MS.gene044004.t1 AT5G55550 27.841 176 112 5 70 233 8 180 3.20e-12 66.6
MS.gene044004.t1 AT5G55550 27.841 176 112 5 70 233 8 180 3.20e-12 66.6
MS.gene044004.t1 AT5G55550 27.841 176 112 5 70 233 8 180 3.20e-12 66.6
MS.gene044004.t1 AT5G55550 27.841 176 112 5 70 233 8 180 3.20e-12 66.6
MS.gene044004.t1 AT5G55550 27.841 176 112 5 70 233 8 180 3.20e-12 66.6
MS.gene044004.t1 AT1G60000 30.263 152 92 4 4 149 114 257 6.42e-12 64.7
MS.gene044004.t1 AT3G52380 27.273 165 97 4 1 149 142 299 7.28e-12 65.1
MS.gene044004.t1 AT3G52380 25.556 180 126 3 70 243 118 295 3.94e-11 63.2
MS.gene044004.t1 AT4G24770 25.424 177 115 3 70 240 152 317 2.10e-11 63.9
MS.gene044004.t1 AT4G24770 25.424 177 115 3 70 240 152 317 2.10e-11 63.9
MS.gene044004.t1 AT3G52150 25.843 178 120 5 70 240 78 250 2.76e-11 62.8
MS.gene044004.t1 AT3G52150 25.843 178 120 5 70 240 78 250 2.76e-11 62.8
MS.gene044004.t1 AT4G13850 35.556 90 57 1 61 150 28 116 3.98e-11 60.1
MS.gene044004.t1 AT4G13850 35.165 91 58 1 61 151 28 117 4.76e-11 60.1
MS.gene044004.t1 AT4G13850 35.165 91 58 1 61 151 28 117 5.70e-11 60.1
MS.gene044004.t1 AT4G13850 35.165 91 58 1 61 151 28 117 6.27e-11 60.1
MS.gene044004.t1 AT3G23830 39.024 82 49 1 70 151 37 117 8.33e-11 59.3
MS.gene044004.t1 AT3G23830 39.024 82 49 1 70 151 37 117 8.33e-11 59.3
MS.gene044004.t1 AT5G09880 25.556 180 119 6 69 241 169 340 9.13e-11 62.4

Find 73 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCCATATCCATCATAAAT+AGG 0.205271 1.3:+3671500 None:intergenic
ACTTGTACCTGTTTGCAAAT+TGG 0.251629 1.3:+3671891 None:intergenic
TAAATAGGGGATGGAAAATA+TGG 0.277141 1.3:+3671515 None:intergenic
ATCCATATCCATCATAAATA+GGG 0.292778 1.3:+3671501 None:intergenic
TCCCCTATTTATGATGGATA+TGG 0.317090 1.3:-3671503 MS.gene044004:CDS
ATCAAGCTTTCCGTTTGAAC+TGG 0.327481 1.3:-3673281 MS.gene044004:CDS
AGCAAACTGTTCGTCTCTCT+TGG 0.360690 1.3:-3671727 MS.gene044004:CDS
AGCGGTATTTCAGAGGGTTA+TGG 0.369552 1.3:-3673381 MS.gene044004:CDS
GCAAACTGTTCGTCTCTCTT+GGG 0.373848 1.3:-3671726 MS.gene044004:CDS
GTTGTTTATGATGCCAATAC+TGG 0.378361 1.3:-3673117 MS.gene044004:CDS
CGGAAAGCTTGATCAGCATT+TGG 0.391386 1.3:+3673291 None:intergenic
GATTCATTATAGATATTTGT+TGG 0.410964 1.3:-3672019 MS.gene044004:intron
TTCAGAGGGTTATGGATTTG+TGG 0.411843 1.3:-3673373 MS.gene044004:CDS
TTTCCATCCCCTATTTATGA+TGG 0.416044 1.3:-3671509 MS.gene044004:CDS
AAGCAGACATTCTCTCAGTA+TGG 0.431532 1.3:-3671962 MS.gene044004:CDS
CCGCAATCCAGCATATAAAC+AGG 0.437492 1.3:-3671702 MS.gene044004:intron
GGCTATGGATTCGTCAGGTT+CGG 0.440915 1.3:-3673084 MS.gene044004:CDS
GCCCTTTGAACGGCCAGTAT+TGG 0.441892 1.3:+3673104 None:intergenic
GAGATTGCCTGTTTATATGC+TGG 0.442204 1.3:+3671695 None:intergenic
GCCAATACTGGCCGTTCAAA+GGG 0.443933 1.3:-3673105 MS.gene044004:CDS
AAGCTAAACGGGACAATGAT+TGG 0.446192 1.3:-3671752 MS.gene044004:CDS
CTCGATGTTGGAAGCTGGGC+CGG 0.446754 1.3:-3671539 MS.gene044004:CDS
AGGCTTTGCAGAAGCTAAAC+GGG 0.447114 1.3:-3671763 MS.gene044004:CDS
TTAGGCTCGATGTTGGAAGC+TGG 0.453457 1.3:-3671544 MS.gene044004:CDS
GAGGCTTTGCAGAAGCTAAA+CGG 0.454111 1.3:-3671764 MS.gene044004:CDS
GATTTGGCATACCTTGTTGA+TGG 0.463014 1.3:+3672959 None:intergenic
AGAATCTGCTTCAGATTGGC+TGG 0.467448 1.3:+3672136 None:intergenic
TCTAGCAGGCCCATGCGTAT+TGG 0.469784 1.3:-3673009 MS.gene044004:CDS
AAACCACAGCCCTTACCAAC+AGG 0.471104 1.3:+3671917 None:intergenic
TATTTAGGAGGTCAGTCAAA+TGG 0.473194 1.3:-3672164 MS.gene044004:intron
GCAACTCCAAGGAAATCATC+TGG 0.473764 1.3:-3672982 MS.gene044004:CDS
CAAAGGGCTATGGATTCGTC+AGG 0.476987 1.3:-3673089 MS.gene044004:CDS
TTTCAGAAATAATGCTGAAG+AGG 0.476993 1.3:-3671783 MS.gene044004:intron
TGATGGCCAGATGATTTCCT+TGG 0.482556 1.3:+3672976 None:intergenic
TGTTAAGATACCTGTTGGTA+AGG 0.488145 1.3:-3671927 MS.gene044004:CDS
TGCCAATACTGGCCGTTCAA+AGG 0.499946 1.3:-3673106 MS.gene044004:CDS
ATACCTGTTGGTAAGGGCTG+TGG 0.509634 1.3:-3671920 MS.gene044004:CDS
TAGGCTCGATGTTGGAAGCT+GGG 0.509979 1.3:-3671543 MS.gene044004:CDS
AGATGAAAGCGAGAGATCTC+AGG 0.512411 1.3:-3673061 MS.gene044004:CDS
TTGTAGAATCTGCTTCAGAT+TGG 0.518743 1.3:+3672132 None:intergenic
AACATTTAGCACAGGAGACA+AGG 0.521571 1.3:-3673256 MS.gene044004:CDS
TCAAGCTTTCCGTTTGAACT+GGG 0.522222 1.3:-3673280 MS.gene044004:CDS
TAAATGTTGCCCAGTTCAAA+CGG 0.531857 1.3:+3673271 None:intergenic
CTGTAAAACTTTCTCAGCTG+TGG 0.534907 1.3:+3673335 None:intergenic
ACTGGCCGTTCAAAGGGCTA+TGG 0.536195 1.3:-3673099 MS.gene044004:CDS
AACTGGGCAACATTTAGCAC+AGG 0.537123 1.3:-3673264 MS.gene044004:CDS
GCCTCTGTTAAGATACCTGT+TGG 0.543628 1.3:-3671932 MS.gene044004:CDS
CTTATTGCGAATAACCTTGA+CGG 0.546836 1.3:+3673407 None:intergenic
CTTGGGTCATGATGAGGAGA+AGG 0.551922 1.3:+3671470 None:intergenic
TAACAGTTTAGGCTCGATGT+TGG 0.554172 1.3:-3671551 MS.gene044004:intron
ACGGTGTTTACTGTTCTAGC+AGG 0.560152 1.3:-3673023 MS.gene044004:CDS
TATCCATCATAAATAGGGGA+TGG 0.565686 1.3:+3671506 None:intergenic
GTTAAGATACCTGTTGGTAA+GGG 0.572861 1.3:-3671926 MS.gene044004:CDS
TCATTATAGATATTTGTTGG+AGG 0.579075 1.3:-3672016 MS.gene044004:intron
CAAACTGTTCGTCTCTCTTG+GGG 0.582336 1.3:-3671725 MS.gene044004:CDS
AGTTGCAGCACCAATACGCA+TGG 0.588428 1.3:+3672999 None:intergenic
TAAGCACAGCGGTATTTCAG+AGG 0.593724 1.3:-3673388 MS.gene044004:CDS
TACATACTTGGGTCATGATG+AGG 0.594093 1.3:+3671464 None:intergenic
AGGGGATGGAAAATATGGAC+CGG 0.598421 1.3:+3671520 None:intergenic
TTTGCAGATTACCTCCGTCA+AGG 0.598780 1.3:-3673421 None:intergenic
TCCATATCCATCATAAATAG+GGG 0.599986 1.3:+3671502 None:intergenic
AAGCACAGCGGTATTTCAGA+GGG 0.617802 1.3:-3673387 MS.gene044004:CDS
GTATTGGTGCTGCAACTCCA+AGG 0.620390 1.3:-3672993 MS.gene044004:CDS
GTTGCAGCACCAATACGCAT+GGG 0.620904 1.3:+3673000 None:intergenic
AAATCATCTGGCCATCAACA+AGG 0.629348 1.3:-3672970 MS.gene044004:intron
CGAATCCATAGCCCTTTGAA+CGG 0.643491 1.3:+3673094 None:intergenic
CCTGTTTATATGCTGGATTG+CGG 0.648162 1.3:+3671702 None:intergenic
TAGCACAGGAGACAAGGGAT+CGG 0.659903 1.3:-3673250 MS.gene044004:CDS
ACATTTAGCACAGGAGACAA+GGG 0.668527 1.3:-3673255 MS.gene044004:CDS
CAGGCTATGACTCAAATGAA+CGG 0.669010 1.3:-3673042 MS.gene044004:CDS
ACCAACAGGTATCTTAACAG+AGG 0.696199 1.3:+3671931 None:intergenic
ATTGCGAATAACCTTGACGG+AGG 0.706195 1.3:+3673410 None:intergenic
GTTATTCGCAATAAGCACAG+CGG 0.787456 1.3:-3673399 MS.gene044004:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGATTAGCTATTTAAAAT+TGG + chr1.3:3672846-3672865 None:intergenic 15.0%
!!! GAAATTTTATTCTTTCATTT+GGG - chr1.3:3671971-3671990 MS.gene044004:CDS 15.0%
!!! TGAAATTTTATTCTTTCATT+TGG - chr1.3:3671970-3671989 MS.gene044004:CDS 15.0%
!! CTTAATAATGATAACAGTTT+AGG - chr1.3:3673323-3673342 MS.gene044004:CDS 20.0%
!! GATTCATTATAGATATTTGT+TGG - chr1.3:3672866-3672885 MS.gene044004:intron 20.0%
! AAGACATAAGAATATCATTC+AGG + chr1.3:3672170-3672189 None:intergenic 25.0%
! ATCCATATCCATCATAAATA+GGG + chr1.3:3673387-3673406 None:intergenic 25.0%
! CAAAAGCAAATAATGTGATT+TGG + chr1.3:3671945-3671964 None:intergenic 25.0%
! GAATCTGAATTCTATAACTT+TGG + chr1.3:3672605-3672624 None:intergenic 25.0%
! GGACTTCAAAAAAAATTATG+AGG + chr1.3:3673243-3673262 None:intergenic 25.0%
! TATAGTCTTCTGTTTAGATT+TGG - chr1.3:3672136-3672155 MS.gene044004:CDS 25.0%
! TATCCATATCCATCATAAAT+AGG + chr1.3:3673388-3673407 None:intergenic 25.0%
! TCAATATGAGGTAATATAGA+TGG + chr1.3:3672045-3672064 None:intergenic 25.0%
! TTTATTCTTTCATTTGGGAA+AGG - chr1.3:3671976-3671995 MS.gene044004:CDS 25.0%
! TTTGTCAAAGACAAATTGAA+GGG + chr1.3:3672500-3672519 None:intergenic 25.0%
!! AAAGTTTTACAGAATTATGC+TGG - chr1.3:3671561-3671580 MS.gene044004:intron 25.0%
!! TCATTATAGATATTTGTTGG+AGG - chr1.3:3672869-3672888 MS.gene044004:intron 25.0%
!! TTTTGTCAAAGACAAATTGA+AGG + chr1.3:3672501-3672520 None:intergenic 25.0%
!!! TTTTGTCTGTTTCCTTATTT+AGG - chr1.3:3672706-3672725 MS.gene044004:intron 25.0%
AGTTACCTCATGAAAATTCT+TGG - chr1.3:3672431-3672450 MS.gene044004:intron 30.0%
CACAAATTCAATTTCATGAC+AGG + chr1.3:3673293-3673312 None:intergenic 30.0%
GGACAAAATCAAAATACAGA+TGG + chr1.3:3672584-3672603 None:intergenic 30.0%
TAAATAGGGGATGGAAAATA+TGG + chr1.3:3673373-3673392 None:intergenic 30.0%
TCCATATCCATCATAAATAG+GGG + chr1.3:3673386-3673405 None:intergenic 30.0%
TTCAGATTCAAATTGCGAAT+CGG - chr1.3:3672616-3672635 MS.gene044004:intron 30.0%
TTTCAGAAATAATGCTGAAG+AGG - chr1.3:3673102-3673121 MS.gene044004:CDS 30.0%
! TGAATCCAAGAATTTTCATG+AGG + chr1.3:3672439-3672458 None:intergenic 30.0%
!! CTTCAGCATTATTTCTGAAA+AGG + chr1.3:3673102-3673121 None:intergenic 30.0%
!!! ACTGATCTGTCTATTTTTGT+AGG - chr1.3:3671666-3671685 MS.gene044004:intron 30.0%
ACTTGTACCTGTTTGCAAAT+TGG + chr1.3:3672997-3673016 None:intergenic 35.0%
AGGACAAAGTGCAAACAATT+TGG + chr1.3:3673273-3673292 None:intergenic 35.0%
AGGTAACTATTTGCCTAATC+TGG + chr1.3:3672419-3672438 None:intergenic 35.0%
ATTGAAGGGATCTAAATGCA+AGG + chr1.3:3672486-3672505 None:intergenic 35.0%
CAGCAGAACAAATCAATATG+AGG + chr1.3:3672057-3672076 None:intergenic 35.0%
CTTATTGCGAATAACCTTGA+CGG + chr1.3:3671481-3671500 None:intergenic 35.0%
GTTGTTTATGATGCCAATAC+TGG - chr1.3:3671768-3671787 MS.gene044004:CDS 35.0%
TAAATGTTGCCCAGTTCAAA+CGG + chr1.3:3671617-3671636 None:intergenic 35.0%
TATCCATCATAAATAGGGGA+TGG + chr1.3:3673382-3673401 None:intergenic 35.0%
TATTTAGGAGGTCAGTCAAA+TGG - chr1.3:3672721-3672740 MS.gene044004:intron 35.0%
TGCAAACAATTTGGTACTCT+AGG + chr1.3:3673264-3673283 None:intergenic 35.0%
TGTTAAGATACCTGTTGGTA+AGG - chr1.3:3672958-3672977 MS.gene044004:intron 35.0%
TTGTAGAATCTGCTTCAGAT+TGG + chr1.3:3672756-3672775 None:intergenic 35.0%
TTTCCATCCCCTATTTATGA+TGG - chr1.3:3673376-3673395 MS.gene044004:CDS 35.0%
TTTGACTGACCTCCTAAATA+AGG + chr1.3:3672721-3672740 None:intergenic 35.0%
! GTCTGCTAGTTTCACTAAAT+AGG - chr1.3:3672287-3672306 MS.gene044004:intron 35.0%
! GTTAAGATACCTGTTGGTAA+GGG - chr1.3:3672959-3672978 MS.gene044004:intron 35.0%
! GTTTTGTCCAATTTGCAAAC+AGG - chr1.3:3672987-3673006 MS.gene044004:CDS 35.0%
! TGTCTGTTTCCTTATTTAGG+AGG - chr1.3:3672709-3672728 MS.gene044004:intron 35.0%
!! ACTTTTGCAGCTTTAACAGA+AGG + chr1.3:3671750-3671769 None:intergenic 35.0%
!! ATATTGATTTGTTCTGCTGC+AGG - chr1.3:3672058-3672077 MS.gene044004:intron 35.0%
!! TCCCCTATTTATGATGGATA+TGG - chr1.3:3673382-3673401 MS.gene044004:CDS 35.0%
AAATCATCTGGCCATCAACA+AGG - chr1.3:3671915-3671934 MS.gene044004:CDS 40.0%
AACATTTAGCACAGGAGACA+AGG - chr1.3:3671629-3671648 MS.gene044004:intron 40.0%
AAGCAGACATTCTCTCAGTA+TGG - chr1.3:3672923-3672942 MS.gene044004:intron 40.0%
AAGCTAAACGGGACAATGAT+TGG - chr1.3:3673133-3673152 MS.gene044004:CDS 40.0%
ACATTTAGCACAGGAGACAA+GGG - chr1.3:3671630-3671649 MS.gene044004:intron 40.0%
ACCAACAGGTATCTTAACAG+AGG + chr1.3:3672957-3672976 None:intergenic 40.0%
ATCAAGCTTTCCGTTTGAAC+TGG - chr1.3:3671604-3671623 MS.gene044004:intron 40.0%
ATCTAAATGCAAGGCACGTT+GGG + chr1.3:3672477-3672496 None:intergenic 40.0%
ATCTTTGGACGGAATGTTCA+AGG + chr1.3:3672014-3672033 None:intergenic 40.0%
CAGGCTATGACTCAAATGAA+CGG - chr1.3:3671843-3671862 MS.gene044004:intron 40.0%
CCTGTTTATATGCTGGATTG+CGG + chr1.3:3673186-3673205 None:intergenic 40.0%
CGAATCGGAAAGTCTATTTC+TGG - chr1.3:3672631-3672650 MS.gene044004:intron 40.0%
CTGTAAAACTTTCTCAGCTG+TGG + chr1.3:3671553-3671572 None:intergenic 40.0%
GAGATTGCCTGTTTATATGC+TGG + chr1.3:3673193-3673212 None:intergenic 40.0%
GATTTGGCATACCTTGTTGA+TGG + chr1.3:3671929-3671948 None:intergenic 40.0%
GTTATTCGCAATAAGCACAG+CGG - chr1.3:3671486-3671505 MS.gene044004:CDS 40.0%
TAACAGTTTAGGCTCGATGT+TGG - chr1.3:3673334-3673353 MS.gene044004:CDS 40.0%
TCAAGCTTTCCGTTTGAACT+GGG - chr1.3:3671605-3671624 MS.gene044004:intron 40.0%
TTCAGAGGGTTATGGATTTG+TGG - chr1.3:3671512-3671531 MS.gene044004:CDS 40.0%
! CAATTTGTTCCATTAGCGTC+TGG - chr1.3:3672377-3672396 MS.gene044004:intron 40.0%
! GATGCTTTTCGATCCAGATT+AGG - chr1.3:3672403-3672422 MS.gene044004:intron 40.0%
!! TAGATGGCTAGAAGCATCTT+TGG + chr1.3:3672029-3672048 None:intergenic 40.0%
AACTGGGCAACATTTAGCAC+AGG - chr1.3:3671621-3671640 MS.gene044004:intron 45.0%
AAGCACAGCGGTATTTCAGA+GGG - chr1.3:3671498-3671517 MS.gene044004:CDS 45.0%
ACGGTGTTTACTGTTCTAGC+AGG - chr1.3:3671862-3671881 MS.gene044004:intron 45.0%
AGAATCTGCTTCAGATTGGC+TGG + chr1.3:3672752-3672771 None:intergenic 45.0%
AGATGAAAGCGAGAGATCTC+AGG - chr1.3:3671824-3671843 MS.gene044004:intron 45.0%
AGCAAACTGTTCGTCTCTCT+TGG - chr1.3:3673158-3673177 MS.gene044004:CDS 45.0%
AGCGGTATTTCAGAGGGTTA+TGG - chr1.3:3671504-3671523 MS.gene044004:CDS 45.0%
AGGCTTTGCAGAAGCTAAAC+GGG - chr1.3:3673122-3673141 MS.gene044004:CDS 45.0%
AGGGGATGGAAAATATGGAC+CGG + chr1.3:3673368-3673387 None:intergenic 45.0%
AGGTTGCTCAAATGTCATCG+TGG + chr1.3:3673082-3673101 None:intergenic 45.0%
ATTGCGAATAACCTTGACGG+AGG + chr1.3:3671478-3671497 None:intergenic 45.0%
CAAACTGTTCGTCTCTCTTG+GGG - chr1.3:3673160-3673179 MS.gene044004:CDS 45.0%
CCGCAATCCAGCATATAAAC+AGG - chr1.3:3673183-3673202 MS.gene044004:CDS 45.0%
CGAATCCATAGCCCTTTGAA+CGG + chr1.3:3671794-3671813 None:intergenic 45.0%
CGGAAAGCTTGATCAGCATT+TGG + chr1.3:3671597-3671616 None:intergenic 45.0%
GAGGCTTTGCAGAAGCTAAA+CGG - chr1.3:3673121-3673140 MS.gene044004:CDS 45.0%
GATCTAAATGCAAGGCACGT+TGG + chr1.3:3672478-3672497 None:intergenic 45.0%
GCAAACTGTTCGTCTCTCTT+GGG - chr1.3:3673159-3673178 MS.gene044004:CDS 45.0%
GCAACTCCAAGGAAATCATC+TGG - chr1.3:3671903-3671922 MS.gene044004:CDS 45.0%
GCCTCTGTTAAGATACCTGT+TGG - chr1.3:3672953-3672972 MS.gene044004:intron 45.0%
TAAGCACAGCGGTATTTCAG+AGG - chr1.3:3671497-3671516 MS.gene044004:CDS 45.0%
TGATGGCCAGATGATTTCCT+TGG + chr1.3:3671912-3671931 None:intergenic 45.0%
TTGCTCAAATGTCATCGTGG+TGG + chr1.3:3673079-3673098 None:intergenic 45.0%
!! TGGCTAGAAGCATCTTTGGA+CGG + chr1.3:3672025-3672044 None:intergenic 45.0%
AAACCACAGCCCTTACCAAC+AGG + chr1.3:3672971-3672990 None:intergenic 50.0%
AGCATCATGCCAGACGCTAA+TGG + chr1.3:3672389-3672408 None:intergenic 50.0%
AGTTGCAGCACCAATACGCA+TGG + chr1.3:3671889-3671908 None:intergenic 50.0%
CAAAGGGCTATGGATTCGTC+AGG - chr1.3:3671796-3671815 MS.gene044004:intron 50.0%
GCCAATACTGGCCGTTCAAA+GGG - chr1.3:3671780-3671799 MS.gene044004:CDS 50.0%
GGCTATGGATTCGTCAGGTT+CGG - chr1.3:3671801-3671820 MS.gene044004:intron 50.0%
GTTGCAGCACCAATACGCAT+GGG + chr1.3:3671888-3671907 None:intergenic 50.0%
TAGCACAGGAGACAAGGGAT+CGG - chr1.3:3671635-3671654 MS.gene044004:intron 50.0%
TAGGCTCGATGTTGGAAGCT+GGG - chr1.3:3673342-3673361 MS.gene044004:CDS 50.0%
TGCCAATACTGGCCGTTCAA+AGG - chr1.3:3671779-3671798 MS.gene044004:CDS 50.0%
TTAGGCTCGATGTTGGAAGC+TGG - chr1.3:3673341-3673360 MS.gene044004:CDS 50.0%
! ATACCTGTTGGTAAGGGCTG+TGG - chr1.3:3672965-3672984 MS.gene044004:intron 50.0%
!! GTATTGGTGCTGCAACTCCA+AGG - chr1.3:3671892-3671911 MS.gene044004:intron 50.0%
ACTGGCCGTTCAAAGGGCTA+TGG - chr1.3:3671786-3671805 MS.gene044004:intron 55.0%
GCCCTTTGAACGGCCAGTAT+TGG + chr1.3:3671784-3671803 None:intergenic 55.0%
TCTAGCAGGCCCATGCGTAT+TGG - chr1.3:3671876-3671895 MS.gene044004:intron 55.0%
CTCGATGTTGGAAGCTGGGC+CGG - chr1.3:3673346-3673365 MS.gene044004:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 3671471 3673436 3671471 ID=MS.gene044004
chr1.3 mRNA 3671471 3673436 3671471 ID=MS.gene044004.t1;Parent=MS.gene044004
chr1.3 exon 3672971 3673436 3672971 ID=MS.gene044004.t1.exon1;Parent=MS.gene044004.t1
chr1.3 CDS 3672971 3673436 3672971 ID=cds.MS.gene044004.t1;Parent=MS.gene044004.t1
chr1.3 exon 3672124 3672179 3672124 ID=MS.gene044004.t1.exon2;Parent=MS.gene044004.t1
chr1.3 CDS 3672124 3672179 3672124 ID=cds.MS.gene044004.t1;Parent=MS.gene044004.t1
chr1.3 exon 3671899 3672029 3671899 ID=MS.gene044004.t1.exon3;Parent=MS.gene044004.t1
chr1.3 CDS 3671899 3672029 3671899 ID=cds.MS.gene044004.t1;Parent=MS.gene044004.t1
chr1.3 exon 3671703 3671799 3671703 ID=MS.gene044004.t1.exon4;Parent=MS.gene044004.t1
chr1.3 CDS 3671703 3671799 3671703 ID=cds.MS.gene044004.t1;Parent=MS.gene044004.t1
chr1.3 exon 3671471 3671567 3671471 ID=MS.gene044004.t1.exon5;Parent=MS.gene044004.t1
chr1.3 CDS 3671471 3671567 3671471 ID=cds.MS.gene044004.t1;Parent=MS.gene044004.t1
Gene Sequence

>MS.gene044004

ATTACCTCCGTCAAGGTTATTCGCAATAAGCACAGCGGTATTTCAGAGGGTTATGGATTTGTGGAGTTCTTTTCACATGCCACAGCTGAGAAAGTTTTACAGAATTATGCTGGAATTTTGATGCCAAATGCTGATCAAGCTTTCCGTTTGAACTGGGCAACATTTAGCACAGGAGACAAGGGATCGGATAATGTCACTGATCTGTCTATTTTTGTAGGAGATTTAGCTGCTGATGTTACAGATAGTGTGTTGCATGAAACTTTTTCTAGTTCATATCCTTCTGTTAAAGCTGCAAAAGTTGTTTATGATGCCAATACTGGCCGTTCAAAGGGCTATGGATTCGTCAGGTTCGGAGATGAAAGCGAGAGATCTCAGGCTATGACTCAAATGAACGGTGTTTACTGTTCTAGCAGGCCCATGCGTATTGGTGCTGCAACTCCAAGGAAATCATCTGGCCATCAACAAGGTATGCCAAATCACATTATTTGCTTTTGTTAAATGAAATTTTATTCTTTCATTTGGGAAAGGAAAAAAATAGTGCCTTGAACATTCCGTCCAAAGATGCTTCTAGCCATCTATATTACCTCATATTGATTTGTTCTGCTGCAGGTTGTTACAGAAGAACTATGATCTGAACTACATTACTTATTTGTATAAGCAGTAACTATAGTCTTCTGTTTAGATTTGGATTTCATTCCTGAATGATATTCTTATGTCTTTCTAAATGAATAGATTTTTTAAAATGACAATTATCACGTCTTTTTCATAGAAAAAAAATTGATATATCATTTACTGAATGCAAATGAAATTGAATCTGTCTGCTAGTTTCACTAAATAGGAGTGAAGATAGTTGAAAAACAATCTAGTTTATTATTTCTTCTGATTATGTAATGAATGCTCAAATGTCAATTTGTTCCATTAGCGTCTGGCATGATGCTTTTCGATCCAGATTAGGCAAATAGTTACCTCATGAAAATTCTTGGATTCATTTTATTGTTTATTTCCCAACGTGCCTTGCATTTAGATCCCTTCAATTTGTCTTTGACAAAACATGTCTCAGTTTTTTTTTTTTTTTATTGCATGCTACTAAAAGATTTTGAAGCATTTGTTCCATCTGTATTTTGATTTTGTCCAAAGTTATAGAATTCAGATTCAAATTGCGAATCGGAAAGTCTATTTCTGGAATTGTGAATTGAATCTGATTATGAATCATATAATATACATGAATATATTTTTTTTGTCTGTTTCCTTATTTAGGAGGTCAGTCAAATGGCACATCCAGCCAATCTGAAGCAGATTCTACAAACACAACAGTGAGCTTTTTCGTTCTTATATTATTAAGTTTTGCATTTTAGAAACTTATCATTTGTTACCAATTTTAAATAGCTAATCAATGATTCATTATAGATATTTGTTGGAGGACTCGACTCTAATGTTACAGATGAAGATCTTAAGCAGACATTCTCTCAGTATGGTGAGATAGCCTCTGTTAAGATACCTGTTGGTAAGGGCTGTGGTTTTGTCCAATTTGCAAACAGGTACAAGTTCTCGACTGTCTCAAATCTTTCATTGATATGCTTTTTAATCTCTTCTTTAATGCAATCACCACCACGATGACATTTGAGCAACCTTTTCAGAAATAATGCTGAAGAGGCTTTGCAGAAGCTAAACGGGACAATGATTGGTAAGCAAACTGTTCGTCTCTCTTGGGGCCGCAATCCAGCATATAAACAGGCAATCTCTCTTTCTCTCTATTTTTCATATCATCTCCTCATAATTTTTTTTGAAGTCCTAGAGTACCAAATTGTTTGCACTTTGTCCTGTCATGAAATTGAATTTGTGCTGATTTTATCTTAATAATGATAACAGTTTAGGCTCGATGTTGGAAGCTGGGCCGGTCCATATTTTCCATCCCCTATTTATGATGGATATGGATATGCAATGCCTTCTCCTCATCATGACCCAA
Protein sequence

>MS.gene044004.t1

ITSVKVIRNKHSGISEGYGFVEFFSHATAEKVLQNYAGILMPNADQAFRLNWATFSTGDKGSDNVTDLSIFVGDLAADVTDSVLHETFSSSYPSVKAAKVVYDANTGRSKGYGFVRFGDESERSQAMTQMNGVYCSSRPMRIGAATPRKSSGHQQGGQSNGTSSQSEADSTNTTIFVGGLDSNVTDEDLKQTFSQYGEIASVKIPVGKGCGFVQFANRNNAEEALQKLNGTMIGKQTVRLSWGRNPAYKQFRLDVGSWAGPYFPSPIYDGYGYAMPSPHHDP