Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069130.t1 | XP_003613023.2 | 92.8 | 414 | 30 | 0 | 1 | 414 | 1 | 414 | 8.60E-204 | 719.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069130.t1 | Q54EY2 | 47.2 | 396 | 182 | 8 | 11 | 405 | 16 | 385 | 1.0e-64 | 248.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069130.t1 | G7JWC0 | 92.8 | 414 | 30 | 0 | 1 | 414 | 1 | 414 | 6.2e-204 | 719.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene069130 | MS.gene22877 | PPI |
| MS.gene069130 | MS.gene031932 | PPI |
| MS.gene069130 | MS.gene015586 | PPI |
| MS.gene069130 | MS.gene040015 | PPI |
| MS.gene069130 | MS.gene019309 | PPI |
| MS.gene069130 | MS.gene040016 | PPI |
| MS.gene043881 | MS.gene069130 | PPI |
| MS.gene069130 | MS.gene024538 | PPI |
| MS.gene069130 | MS.gene050052 | PPI |
| MS.gene069130 | MS.gene008016 | PPI |
| MS.gene069130 | MS.gene50744 | PPI |
| MS.gene069130 | MS.gene072782 | PPI |
| MS.gene069130 | MS.gene031933 | PPI |
| MS.gene025997 | MS.gene069130 | PPI |
| MS.gene64127 | MS.gene069130 | PPI |
| MS.gene67432 | MS.gene069130 | PPI |
| MS.gene003693 | MS.gene069130 | PPI |
| MS.gene069130 | MS.gene050556 | PPI |
| MS.gene069130 | MS.gene010144 | PPI |
| MS.gene069130 | MS.gene67431 | PPI |
| MS.gene069130 | MS.gene008015 | PPI |
| MS.gene069130 | MS.gene23314 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069130.t1 | MTR_5g031790 | 99.034 | 414 | 4 | 0 | 1 | 414 | 1 | 414 | 0.0 | 835 |
| MS.gene069130.t1 | MTR_5g031790 | 98.500 | 400 | 6 | 0 | 15 | 414 | 12 | 411 | 0.0 | 799 |
| MS.gene069130.t1 | MTR_8g023040 | 29.130 | 230 | 138 | 3 | 160 | 389 | 144 | 348 | 1.06e-19 | 90.1 |
| MS.gene069130.t1 | MTR_6g017100 | 28.095 | 210 | 135 | 4 | 198 | 398 | 262 | 464 | 1.02e-16 | 82.4 |
| MS.gene069130.t1 | MTR_6g017100 | 28.095 | 210 | 135 | 4 | 198 | 398 | 442 | 644 | 3.34e-16 | 81.3 |
| MS.gene069130.t1 | MTR_2g055750 | 26.446 | 242 | 150 | 5 | 176 | 398 | 218 | 450 | 9.12e-16 | 79.3 |
| MS.gene069130.t1 | MTR_2g055750 | 26.446 | 242 | 150 | 5 | 176 | 398 | 141 | 373 | 1.20e-15 | 78.6 |
| MS.gene069130.t1 | MTR_2g055750 | 26.446 | 242 | 150 | 5 | 176 | 398 | 397 | 629 | 1.31e-15 | 79.3 |
| MS.gene069130.t1 | MTR_2g055750 | 27.193 | 228 | 138 | 5 | 176 | 384 | 141 | 359 | 2.68e-15 | 77.0 |
| MS.gene069130.t1 | MTR_2g055750 | 27.193 | 228 | 138 | 5 | 176 | 384 | 218 | 436 | 2.75e-15 | 77.8 |
| MS.gene069130.t1 | MTR_2g055750 | 27.193 | 228 | 138 | 5 | 176 | 384 | 397 | 615 | 6.25e-15 | 77.0 |
| MS.gene069130.t1 | MTR_7g018590 | 31.100 | 209 | 130 | 3 | 198 | 398 | 409 | 611 | 3.74e-14 | 74.7 |
| MS.gene069130.t1 | MTR_2g042450 | 36.601 | 153 | 89 | 6 | 236 | 384 | 203 | 351 | 1.15e-13 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069130.t1 | AT3G07300 | 81.418 | 409 | 71 | 3 | 1 | 406 | 1 | 407 | 0.0 | 649 |
| MS.gene069130.t1 | AT3G07300 | 81.418 | 409 | 71 | 3 | 1 | 406 | 1 | 407 | 0.0 | 649 |
| MS.gene069130.t1 | AT3G07300 | 81.907 | 409 | 71 | 2 | 1 | 406 | 1 | 409 | 0.0 | 635 |
| MS.gene069130.t1 | AT1G53880 | 34.375 | 192 | 115 | 2 | 198 | 389 | 194 | 374 | 2.38e-21 | 95.5 |
| MS.gene069130.t1 | AT2G44070 | 29.694 | 229 | 149 | 3 | 176 | 398 | 98 | 320 | 5.52e-21 | 93.6 |
| MS.gene069130.t1 | AT1G53900 | 34.375 | 192 | 115 | 2 | 198 | 389 | 389 | 569 | 1.53e-20 | 94.4 |
| MS.gene069130.t1 | AT1G53900 | 34.375 | 192 | 115 | 2 | 198 | 389 | 413 | 593 | 1.65e-20 | 94.4 |
| MS.gene069130.t1 | AT1G53900 | 34.375 | 192 | 115 | 2 | 198 | 389 | 404 | 584 | 1.71e-20 | 94.4 |
| MS.gene069130.t1 | AT1G72340 | 32.812 | 192 | 118 | 2 | 198 | 389 | 191 | 371 | 1.06e-19 | 90.5 |
| MS.gene069130.t1 | AT1G72340 | 32.812 | 192 | 118 | 2 | 198 | 389 | 218 | 398 | 2.06e-19 | 90.1 |
| MS.gene069130.t1 | AT5G38640 | 29.004 | 231 | 147 | 5 | 176 | 398 | 414 | 635 | 1.48e-18 | 88.6 |
| MS.gene069130.t1 | AT1G48970 | 27.542 | 236 | 144 | 5 | 182 | 398 | 432 | 659 | 2.62e-18 | 87.8 |
| MS.gene069130.t1 | AT1G48970 | 28.061 | 196 | 119 | 4 | 182 | 368 | 432 | 614 | 4.40e-14 | 74.7 |
| MS.gene069130.t1 | AT1G72340 | 36.000 | 125 | 80 | 0 | 198 | 322 | 191 | 315 | 1.23e-13 | 72.0 |
Find 95 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCCCAGTTGGATTAAATA+TGG | 0.261046 | 5.3:-20519377 | MS.gene069130:CDS |
| CATCTGAGCTACTTGACTTT+GGG | 0.277211 | 5.3:-20516978 | MS.gene069130:CDS |
| CATTCCACACACATCTTCTT+CGG | 0.279214 | 5.3:-20522422 | MS.gene069130:CDS |
| TCAGGATTGTGTGGATATAA+AGG | 0.314969 | 5.3:+20517027 | None:intergenic |
| ATAAAGAGACTAACAAGTTT+TGG | 0.329435 | 5.3:+20516880 | None:intergenic |
| CTACTACTCAGCTGATGATT+TGG | 0.361094 | 5.3:-20516691 | MS.gene069130:CDS |
| CTTACAAACCACAGTGATTA+CGG | 0.362996 | 5.3:-20519618 | MS.gene069130:CDS |
| CCATCTGAGCTACTTGACTT+TGG | 0.380771 | 5.3:-20516979 | MS.gene069130:CDS |
| TTCTCAGGCCACAGCAAAAC+AGG | 0.391585 | 5.3:-20524488 | MS.gene069130:CDS |
| CTCGCCACAGCTGCTATAGA+TGG | 0.395990 | 5.3:-20522598 | MS.gene069130:CDS |
| AGCTCATTCAATAGGACTTC+AGG | 0.399080 | 5.3:+20517009 | None:intergenic |
| ACATCTCCTTGCAAAGGAAT+TGG | 0.400608 | 5.3:-20519651 | MS.gene069130:CDS |
| GCAACCATATTTAATCCAAC+TGG | 0.404453 | 5.3:+20519373 | None:intergenic |
| GCCATTATATAGGTTATAGT+TGG | 0.411016 | 5.3:-20519433 | MS.gene069130:intron |
| GCTCATGCTGTCATGGCTAA+TGG | 0.417180 | 5.3:-20519409 | MS.gene069130:CDS |
| TGTGGAATGGCAGCTGAAAT+AGG | 0.420775 | 5.3:+20522436 | None:intergenic |
| ATTCCACACACATCTTCTTC+GGG | 0.421889 | 5.3:-20522421 | MS.gene069130:CDS |
| ACTTGACTTTGGGGAGTTCT+CGG | 0.423510 | 5.3:-20516968 | MS.gene069130:CDS |
| CACAGCTGCTATAGATGGTT+TGG | 0.431387 | 5.3:-20522593 | MS.gene069130:CDS |
| GTGGCACATAATCGAATGTT+GGG | 0.433718 | 5.3:+20516902 | None:intergenic |
| AACAGGCAGCAGAGTTGCTT+CGG | 0.442571 | 5.3:-20524471 | MS.gene069130:CDS |
| TCTAGAAGAGTTTGCAATGA+TGG | 0.447595 | 5.3:+20522466 | None:intergenic |
| GGGATCTCAGCTCATTCAAT+AGG | 0.448684 | 5.3:+20517001 | None:intergenic |
| CAGCTCTGATTAATGCAGTA+AGG | 0.457689 | 5.3:-20524405 | MS.gene069130:CDS |
| CATGTTCACTCGGGAAATCA+TGG | 0.464512 | 5.3:+20519580 | None:intergenic |
| CAGGATTGTGTGGATATAAA+GGG | 0.475593 | 5.3:+20517028 | None:intergenic |
| TAGTAAAATTGAAGGTTCTC+AGG | 0.480222 | 5.3:-20524503 | MS.gene069130:CDS |
| TTAACAAGGGCATGAACATC+CGG | 0.483965 | 5.3:+20524723 | None:intergenic |
| ATGAGCCGGTACATGTATGA+TGG | 0.490186 | 5.3:+20516720 | None:intergenic |
| GGTGGCACATAATCGAATGT+TGG | 0.494049 | 5.3:+20516901 | None:intergenic |
| TTTAAGGTCTTTGTTGCTGA+AGG | 0.501556 | 5.3:-20519801 | MS.gene069130:CDS |
| ACAGCAGCAGCAGATAAAAC+AGG | 0.508963 | 5.3:+20522520 | None:intergenic |
| TCCACACACATCTTCTTCGG+GGG | 0.512899 | 5.3:-20522419 | MS.gene069130:CDS |
| ATCTGAGCTACTTGACTTTG+GGG | 0.516470 | 5.3:-20516977 | MS.gene069130:CDS |
| TGTGATGCTAGTAAAATTGA+AGG | 0.519197 | 5.3:-20524511 | MS.gene069130:intron |
| TGAACTCAACTCGTGGTGGC+AGG | 0.522063 | 5.3:+20516654 | None:intergenic |
| TGGGGAGTTCTCGGATTGCA+TGG | 0.522920 | 5.3:-20516959 | MS.gene069130:CDS |
| CATGATTTCCCGAGTGAACA+TGG | 0.523546 | 5.3:-20519579 | MS.gene069130:intron |
| ATCTATAGCAGCTGTGGCGA+GGG | 0.523810 | 5.3:+20522600 | None:intergenic |
| CCAAAGTCAAGTAGCTCAGA+TGG | 0.527577 | 5.3:+20516979 | None:intergenic |
| ATTAATGCAGTAAGGGCTGT+GGG | 0.530555 | 5.3:-20524397 | MS.gene069130:CDS |
| CTTCCAGCAAGTACAACAAA+AGG | 0.532130 | 5.3:+20519325 | None:intergenic |
| TTACATGCAATCAGATATCA+AGG | 0.533256 | 5.3:-20519674 | MS.gene069130:intron |
| GATTAATGCAGTAAGGGCTG+TGG | 0.538174 | 5.3:-20524398 | MS.gene069130:CDS |
| AGTTGGAGCTCATGCTGTCA+TGG | 0.544079 | 5.3:-20519416 | MS.gene069130:CDS |
| CATTAATCAGAGCTGTAGCC+TGG | 0.544553 | 5.3:+20524413 | None:intergenic |
| CAACCATATTTAATCCAACT+GGG | 0.549372 | 5.3:+20519374 | None:intergenic |
| CATCTATAGCAGCTGTGGCG+AGG | 0.558794 | 5.3:+20522599 | None:intergenic |
| CTACTCAGCTGATGATTTGG+TGG | 0.560163 | 5.3:-20516688 | MS.gene069130:CDS |
| AGCTCTGATTAATGCAGTAA+GGG | 0.560416 | 5.3:-20524404 | MS.gene069130:CDS |
| GGTGGAGTAATTGCCCCAGT+TGG | 0.564989 | 5.3:-20519388 | MS.gene069130:CDS |
| TTCCACACACATCTTCTTCG+GGG | 0.565199 | 5.3:-20522420 | MS.gene069130:CDS |
| GCTGTAGCCTGGTGTGTTTG+AGG | 0.565811 | 5.3:+20524424 | None:intergenic |
| AATAGGACTTCAGGATTGTG+TGG | 0.568015 | 5.3:+20517018 | None:intergenic |
| TAGCAGCCAATTCCTTTGCA+AGG | 0.568086 | 5.3:+20519645 | None:intergenic |
| TTCACATACCATGTTCACTC+GGG | 0.568752 | 5.3:+20519571 | None:intergenic |
| TTGTTGCTGAAGGTGCCCCA+AGG | 0.574437 | 5.3:-20519791 | MS.gene069130:intron |
| CAAAGTCAAGTAGCTCAGAT+GGG | 0.575196 | 5.3:+20516980 | None:intergenic |
| TGGTTGCACTTGCAGCTCAA+AGG | 0.575917 | 5.3:-20519357 | MS.gene069130:CDS |
| GCAAAGGAATTGGCTGCTAG+AGG | 0.576383 | 5.3:-20519641 | MS.gene069130:CDS |
| CTTATCGCTGCTAACCCTGT+TGG | 0.576513 | 5.3:-20524367 | MS.gene069130:intron |
| ATAACCCATCATACATGTAC+CGG | 0.581574 | 5.3:-20516725 | MS.gene069130:CDS |
| TTGATAAGAGTTCAAGAGGT+CGG | 0.582725 | 5.3:-20521677 | MS.gene069130:CDS |
| GCTGTTGATAAGAGTTCAAG+AGG | 0.585356 | 5.3:-20521681 | MS.gene069130:CDS |
| AGTGAGGTAATATTAACGCT+AGG | 0.588396 | 5.3:-20519879 | MS.gene069130:CDS |
| GGTCTGTGATTTCACAGCAG+AGG | 0.588960 | 5.3:-20524450 | MS.gene069130:CDS |
| TGAGCCGGTACATGTATGAT+GGG | 0.590459 | 5.3:+20516721 | None:intergenic |
| AGTAGTAGTCAGCAATGAGC+CGG | 0.599358 | 5.3:+20516706 | None:intergenic |
| TCAAGGACATCTCCTTGCAA+AGG | 0.600599 | 5.3:-20519657 | MS.gene069130:CDS |
| AGGAAGCTCAAAGACCGTGT+TGG | 0.600809 | 5.3:-20519859 | MS.gene069130:CDS |
| TAATGCAGTAAGGGCTGTGG+GGG | 0.601574 | 5.3:-20524395 | MS.gene069130:CDS |
| GATTGCATGGATTCTGCAAG+TGG | 0.604574 | 5.3:-20516946 | MS.gene069130:CDS |
| ATCATTGCAAACTCTTCTAG+AGG | 0.605650 | 5.3:-20522464 | MS.gene069130:CDS |
| TTTGCACATTATAAGAGAGG+AGG | 0.613292 | 5.3:-20522629 | MS.gene069130:CDS |
| CGAAGAAGATGTGTGTGGAA+TGG | 0.615874 | 5.3:+20522423 | None:intergenic |
| TGCATGGATTCTGCAAGTGG+AGG | 0.616289 | 5.3:-20516943 | MS.gene069130:CDS |
| TTTCACATACCATGTTCACT+CGG | 0.618413 | 5.3:+20519570 | None:intergenic |
| AGAAGAGTTTGCAATGATGG+TGG | 0.621504 | 5.3:+20522469 | None:intergenic |
| TGCGATTGCAGAGCTTGCTG+TGG | 0.623208 | 5.3:-20522671 | MS.gene069130:intron |
| CATGCTGTCATGGCTAATGG+TGG | 0.624533 | 5.3:-20519406 | MS.gene069130:CDS |
| CAAACCATCTATAGCAGCTG+TGG | 0.635061 | 5.3:+20522594 | None:intergenic |
| TTGCAATGATGGTGGACGCA+AGG | 0.636061 | 5.3:+20522477 | None:intergenic |
| CCACACACATCTTCTTCGGG+GGG | 0.636249 | 5.3:-20522418 | MS.gene069130:CDS |
| CAGCTGAACTCAACTCGTGG+TGG | 0.648194 | 5.3:+20516650 | None:intergenic |
| AGGCAGCTGAACTCAACTCG+TGG | 0.669429 | 5.3:+20516647 | None:intergenic |
| GAGGTTGCCTCAAACACACC+AGG | 0.669449 | 5.3:-20524431 | MS.gene069130:CDS |
| AAAGTCAAGTAGCTCAGATG+GGG | 0.670560 | 5.3:+20516981 | None:intergenic |
| GCGATTGCAGAGCTTGCTGT+GGG | 0.680652 | 5.3:-20522670 | MS.gene069130:intron |
| TCCATCTCAGCATACCAACA+GGG | 0.686122 | 5.3:+20524353 | None:intergenic |
| TTAATGCAGTAAGGGCTGTG+GGG | 0.689310 | 5.3:-20524396 | MS.gene069130:CDS |
| TGTACTTGCTGGAAGTCACA+AGG | 0.692857 | 5.3:-20519317 | MS.gene069130:intron |
| AACCATATTTAATCCAACTG+GGG | 0.718901 | 5.3:+20519375 | None:intergenic |
| AGCACAAAGAAATTCCAACA+CGG | 0.736757 | 5.3:+20519845 | None:intergenic |
| TTGCTGTGGGAAATATTGTG+AGG | 0.744546 | 5.3:-20522657 | MS.gene069130:CDS |
| TGCAGAATCCGTAATCACTG+TGG | 0.748569 | 5.3:+20519610 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 20516659 | 20524748 | 20516659 | ID=MS.gene069130 |
| chr5.3 | mRNA | 20516659 | 20524748 | 20516659 | ID=MS.gene069130.t1;Parent=MS.gene069130 |
| chr5.3 | exon | 20524696 | 20524748 | 20524696 | ID=MS.gene069130.t1.exon1;Parent=MS.gene069130.t1 |
| chr5.3 | CDS | 20524696 | 20524748 | 20524696 | ID=cds.MS.gene069130.t1;Parent=MS.gene069130.t1 |
| chr5.3 | exon | 20524368 | 20524522 | 20524368 | ID=MS.gene069130.t1.exon2;Parent=MS.gene069130.t1 |
| chr5.3 | CDS | 20524368 | 20524522 | 20524368 | ID=cds.MS.gene069130.t1;Parent=MS.gene069130.t1 |
| chr5.3 | exon | 20522395 | 20522682 | 20522395 | ID=MS.gene069130.t1.exon3;Parent=MS.gene069130.t1 |
| chr5.3 | CDS | 20522395 | 20522682 | 20522395 | ID=cds.MS.gene069130.t1;Parent=MS.gene069130.t1 |
| chr5.3 | exon | 20521573 | 20521702 | 20521573 | ID=MS.gene069130.t1.exon4;Parent=MS.gene069130.t1 |
| chr5.3 | CDS | 20521573 | 20521702 | 20521573 | ID=cds.MS.gene069130.t1;Parent=MS.gene069130.t1 |
| chr5.3 | exon | 20519792 | 20519899 | 20519792 | ID=MS.gene069130.t1.exon5;Parent=MS.gene069130.t1 |
| chr5.3 | CDS | 20519792 | 20519899 | 20519792 | ID=cds.MS.gene069130.t1;Parent=MS.gene069130.t1 |
| chr5.3 | exon | 20519580 | 20519682 | 20519580 | ID=MS.gene069130.t1.exon6;Parent=MS.gene069130.t1 |
| chr5.3 | CDS | 20519580 | 20519682 | 20519580 | ID=cds.MS.gene069130.t1;Parent=MS.gene069130.t1 |
| chr5.3 | exon | 20519318 | 20519443 | 20519318 | ID=MS.gene069130.t1.exon7;Parent=MS.gene069130.t1 |
| chr5.3 | CDS | 20519318 | 20519443 | 20519318 | ID=cds.MS.gene069130.t1;Parent=MS.gene069130.t1 |
| chr5.3 | exon | 20516871 | 20517055 | 20516871 | ID=MS.gene069130.t1.exon8;Parent=MS.gene069130.t1 |
| chr5.3 | CDS | 20516871 | 20517055 | 20516871 | ID=cds.MS.gene069130.t1;Parent=MS.gene069130.t1 |
| chr5.3 | exon | 20516659 | 20516755 | 20516659 | ID=MS.gene069130.t1.exon9;Parent=MS.gene069130.t1 |
| chr5.3 | CDS | 20516659 | 20516755 | 20516659 | ID=cds.MS.gene069130.t1;Parent=MS.gene069130.t1 |
| Gene Sequence |
| Protein sequence |
