Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073028.t1 | XP_024638328.1 | 89.4 | 104 | 11 | 0 | 1 | 104 | 51 | 154 | 7.20E-46 | 193 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073028.t1 | A0A396GQG1 | 87.5 | 104 | 13 | 0 | 1 | 104 | 1 | 104 | 7.5e-45 | 189.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene073028 | 0.803699 | 3.08E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene073028 | -0.804305 | 2.30E-49 | -1.69E-46 |
| MS.gene049669 | MS.gene073028 | 0.811726 | 6.06E-51 | -1.69E-46 |
| MS.gene051935 | MS.gene073028 | 0.805745 | 1.15E-49 | -1.69E-46 |
| MS.gene051975 | MS.gene073028 | 0.815167 | 1.06E-51 | -1.69E-46 |
| MS.gene052108 | MS.gene073028 | 0.807378 | 5.20E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene073028 | 0.80321 | 3.89E-49 | -1.69E-46 |
| MS.gene052685 | MS.gene073028 | 0.822094 | 2.85E-53 | -1.69E-46 |
| MS.gene052889 | MS.gene073028 | 0.814915 | 1.21E-51 | -1.69E-46 |
| MS.gene053298 | MS.gene073028 | 0.828602 | 8.20E-55 | -1.69E-46 |
| MS.gene053340 | MS.gene073028 | 0.841358 | 4.99E-58 | -1.69E-46 |
| MS.gene053963 | MS.gene073028 | 0.846008 | 2.84E-59 | -1.69E-46 |
| MS.gene054222 | MS.gene073028 | 0.825293 | 5.07E-54 | -1.69E-46 |
| MS.gene054479 | MS.gene073028 | 0.834234 | 3.37E-56 | -1.69E-46 |
| MS.gene054576 | MS.gene073028 | 0.80752 | 4.86E-50 | -1.69E-46 |
| MS.gene055313 | MS.gene073028 | 0.805963 | 1.04E-49 | -1.69E-46 |
| MS.gene055603 | MS.gene073028 | 0.846132 | 2.63E-59 | -1.69E-46 |
| MS.gene056403 | MS.gene073028 | 0.823208 | 1.57E-53 | -1.69E-46 |
| MS.gene05659 | MS.gene073028 | 0.812105 | 5.01E-51 | -1.69E-46 |
| MS.gene056605 | MS.gene073028 | 0.808868 | 2.51E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene073028 | 0.840157 | 1.03E-57 | -1.69E-46 |
| MS.gene057026 | MS.gene073028 | 0.806404 | 8.36E-50 | -1.69E-46 |
| MS.gene058498 | MS.gene073028 | 0.814478 | 1.51E-51 | -1.69E-46 |
| MS.gene060365 | MS.gene073028 | 0.801489 | 8.79E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene073028 | 0.827718 | 1.34E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073028.t1 | MTR_1g094760 | 72.277 | 101 | 28 | 0 | 1 | 101 | 436 | 536 | 1.74e-47 | 161 |
| MS.gene073028.t1 | MTR_8g085310 | 68.317 | 101 | 32 | 0 | 1 | 101 | 422 | 522 | 8.79e-44 | 150 |
| MS.gene073028.t1 | MTR_4g092300 | 67.327 | 101 | 33 | 0 | 1 | 101 | 544 | 644 | 2.37e-41 | 144 |
| MS.gene073028.t1 | MTR_4g051480 | 67.327 | 101 | 33 | 0 | 1 | 101 | 562 | 662 | 3.43e-41 | 144 |
| MS.gene073028.t1 | MTR_3g065345 | 82.353 | 68 | 12 | 0 | 34 | 101 | 1 | 68 | 4.61e-38 | 124 |
| MS.gene073028.t1 | MTR_3g053640 | 53.774 | 106 | 39 | 2 | 1 | 101 | 170 | 270 | 1.06e-28 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAAGCATAACACCGGATT+TGG | 0.286208 | 3.4:+94422210 | MS.gene073028:CDS |
| AAAACTTTCGTTACAAAATC+AGG | 0.317317 | 3.4:-94422009 | None:intergenic |
| ATCTTTGCCTTTAGTTGATC+AGG | 0.321053 | 3.4:-94422150 | None:intergenic |
| CTAGTATGAAACACACCATT+TGG | 0.419336 | 3.4:-94422072 | None:intergenic |
| GTTACTGCAACTGAAGAATC+TGG | 0.425594 | 3.4:+94422255 | MS.gene073028:CDS |
| TTGATACGGTCGATTAGTTA+TGG | 0.456557 | 3.4:-94422127 | None:intergenic |
| TTCAGCATTCCAGCCAAATC+CGG | 0.456604 | 3.4:-94422223 | None:intergenic |
| AGCATAACACCGGATTTGGC+TGG | 0.484895 | 3.4:+94422214 | MS.gene073028:CDS |
| ATGGTTGATGAAGTTACAAA+AGG | 0.489652 | 3.4:+94422042 | MS.gene073028:CDS |
| ACAAAAGGAAATATGCCAAA+TGG | 0.496204 | 3.4:+94422057 | MS.gene073028:CDS |
| ACATTAATGAGACAAAGCTT+TGG | 0.497947 | 3.4:+94421986 | MS.gene073028:CDS |
| TTTAGTTGATCAGGTTGATA+CGG | 0.511505 | 3.4:-94422141 | None:intergenic |
| TGAATAGAACTCACGATACA+TGG | 0.530708 | 3.4:-94422187 | None:intergenic |
| GTATCAACCTGATCAACTAA+AGG | 0.566076 | 3.4:+94422143 | MS.gene073028:CDS |
| AACTAAAGGCAAAGATGCAT+AGG | 0.582306 | 3.4:+94422157 | MS.gene073028:CDS |
| ACTCAACTTATACATCATTG+AGG | 0.624896 | 3.4:-94422103 | None:intergenic |
| GTGTGTTTCATACTAGAACA+TGG | 0.658064 | 3.4:+94422079 | MS.gene073028:CDS |
| TATTCACTGAAGCATAACAC+CGG | 0.679933 | 3.4:+94422204 | MS.gene073028:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAACTTTCGTTACAAAATC+AGG | - | chr3.4:94422012-94422031 | None:intergenic | 25.0% |
| !! | GAAAGTTTTCATTGACATAA+TGG | + | chr3.4:94422023-94422042 | MS.gene073028:CDS | 25.0% |
| ACAAAAGGAAATATGCCAAA+TGG | + | chr3.4:94422057-94422076 | MS.gene073028:CDS | 30.0% | |
| ACATTAATGAGACAAAGCTT+TGG | + | chr3.4:94421986-94422005 | MS.gene073028:CDS | 30.0% | |
| ACTCAACTTATACATCATTG+AGG | - | chr3.4:94422106-94422125 | None:intergenic | 30.0% | |
| !! | ATGGTTGATGAAGTTACAAA+AGG | + | chr3.4:94422042-94422061 | MS.gene073028:CDS | 30.0% |
| !! | TTTAGTTGATCAGGTTGATA+CGG | - | chr3.4:94422144-94422163 | None:intergenic | 30.0% |
| AACTAAAGGCAAAGATGCAT+AGG | + | chr3.4:94422157-94422176 | MS.gene073028:CDS | 35.0% | |
| ATCTTTGCCTTTAGTTGATC+AGG | - | chr3.4:94422153-94422172 | None:intergenic | 35.0% | |
| CTAGTATGAAACACACCATT+TGG | - | chr3.4:94422075-94422094 | None:intergenic | 35.0% | |
| GTATCAACCTGATCAACTAA+AGG | + | chr3.4:94422143-94422162 | MS.gene073028:CDS | 35.0% | |
| GTGTGTTTCATACTAGAACA+TGG | + | chr3.4:94422079-94422098 | MS.gene073028:CDS | 35.0% | |
| TATTCACTGAAGCATAACAC+CGG | + | chr3.4:94422204-94422223 | MS.gene073028:CDS | 35.0% | |
| TGAATAGAACTCACGATACA+TGG | - | chr3.4:94422190-94422209 | None:intergenic | 35.0% | |
| ! | TTGATACGGTCGATTAGTTA+TGG | - | chr3.4:94422130-94422149 | None:intergenic | 35.0% |
| GTTACTGCAACTGAAGAATC+TGG | + | chr3.4:94422255-94422274 | MS.gene073028:CDS | 40.0% | |
| CTGAAGCATAACACCGGATT+TGG | + | chr3.4:94422210-94422229 | MS.gene073028:CDS | 45.0% | |
| TTCAGCATTCCAGCCAAATC+CGG | - | chr3.4:94422226-94422245 | None:intergenic | 45.0% | |
| AGCATAACACCGGATTTGGC+TGG | + | chr3.4:94422214-94422233 | MS.gene073028:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 94421970 | 94422290 | 94421970 | ID=MS.gene073028 |
| chr3.4 | mRNA | 94421970 | 94422290 | 94421970 | ID=MS.gene073028.t1;Parent=MS.gene073028 |
| chr3.4 | exon | 94421970 | 94422290 | 94421970 | ID=MS.gene073028.t1.exon1;Parent=MS.gene073028.t1 |
| chr3.4 | CDS | 94421970 | 94422290 | 94421970 | ID=cds.MS.gene073028.t1;Parent=MS.gene073028.t1 |
| Gene Sequence |
| Protein sequence |
