Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073029.t1 | XP_024638328.1 | 95.7 | 188 | 8 | 0 | 6 | 193 | 158 | 345 | 1.10E-97 | 365.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073029.t1 | A0A396GQG1 | 94.7 | 188 | 10 | 0 | 6 | 193 | 108 | 295 | 5.8e-96 | 359.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050355 | MS.gene073029 | 0.813481 | 2.50E-51 | -1.69E-46 |
| MS.gene051935 | MS.gene073029 | 0.810695 | 1.01E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene073029 | 0.805249 | 1.46E-49 | -1.69E-46 |
| MS.gene052108 | MS.gene073029 | 0.800687 | 1.28E-48 | -1.69E-46 |
| MS.gene052333 | MS.gene073029 | 0.805601 | 1.23E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene073029 | 0.80236 | 5.82E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene073029 | 0.812036 | 5.19E-51 | -1.69E-46 |
| MS.gene052685 | MS.gene073029 | 0.810634 | 1.05E-50 | -1.69E-46 |
| MS.gene052773 | MS.gene073029 | 0.821408 | 4.10E-53 | -1.69E-46 |
| MS.gene052954 | MS.gene073029 | 0.80581 | 1.12E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene073029 | 0.829866 | 4.05E-55 | -1.69E-46 |
| MS.gene053963 | MS.gene073029 | 0.80356 | 3.29E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene073029 | 0.808639 | 2.81E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene073029 | 0.80006 | 1.72E-48 | -1.69E-46 |
| MS.gene055166 | MS.gene073029 | 0.800522 | 1.39E-48 | -1.69E-46 |
| MS.gene055586 | MS.gene073029 | 0.810445 | 1.15E-50 | -1.69E-46 |
| MS.gene055603 | MS.gene073029 | 0.825467 | 4.61E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene073029 | 0.805828 | 1.11E-49 | -1.69E-46 |
| MS.gene056162 | MS.gene073029 | 0.810366 | 1.19E-50 | -1.69E-46 |
| MS.gene05659 | MS.gene073029 | 0.808176 | 3.52E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene073029 | 0.82268 | 2.08E-53 | -1.69E-46 |
| MS.gene057026 | MS.gene073029 | 0.829738 | 4.35E-55 | -1.69E-46 |
| MS.gene057208 | MS.gene073029 | 0.805994 | 1.02E-49 | -1.69E-46 |
| MS.gene058498 | MS.gene073029 | 0.832768 | 7.83E-56 | -1.69E-46 |
| MS.gene059935 | MS.gene073029 | 0.802791 | 4.74E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene073029 | 0.807871 | 4.09E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene073029 | 0.834267 | 3.31E-56 | -1.69E-46 |
| MS.gene060586 | MS.gene073029 | 0.813041 | 3.13E-51 | -1.69E-46 |
| MS.gene060879 | MS.gene073029 | 0.814934 | 1.20E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene073029 | -0.802114 | 6.54E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073029.t1 | MTR_4g092300 | 59.585 | 193 | 59 | 4 | 5 | 193 | 650 | 827 | 1.04e-65 | 216 |
| MS.gene073029.t1 | MTR_4g051480 | 59.585 | 193 | 59 | 4 | 5 | 193 | 668 | 845 | 1.08e-65 | 216 |
| MS.gene073029.t1 | MTR_8g085310 | 55.670 | 194 | 69 | 4 | 5 | 193 | 528 | 709 | 5.71e-62 | 204 |
| MS.gene073029.t1 | MTR_1g094760 | 57.303 | 178 | 57 | 4 | 20 | 193 | 540 | 702 | 1.32e-53 | 181 |
| MS.gene073029.t1 | MTR_3g053640 | 48.677 | 189 | 66 | 4 | 5 | 193 | 276 | 433 | 4.11e-50 | 167 |
| MS.gene073029.t1 | MTR_3g019060 | 36.970 | 165 | 67 | 4 | 30 | 193 | 5 | 133 | 6.09e-25 | 94.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCGTTGGAAAAGTTTA+AGG | 0.170316 | 3.4:+94423163 | MS.gene073029:CDS |
| TGATATGCATATATCATTTC+AGG | 0.239157 | 3.4:+94422671 | None:intergenic |
| CATCTGTGTTTGGTGGGTCT+TGG | 0.324547 | 3.4:-94422803 | None:intergenic |
| AGCATGGGCTCAAGTTTCTT+TGG | 0.334501 | 3.4:+94423013 | MS.gene073029:CDS |
| ATGTGATGTAGACTCCTTCT+TGG | 0.355967 | 3.4:-94422970 | None:intergenic |
| ATCTGTGTTTGGTGGGTCTT+GGG | 0.406768 | 3.4:-94422802 | None:intergenic |
| GTGTGACCATTACAAGTTGC+TGG | 0.423848 | 3.4:+94422728 | MS.gene073029:CDS |
| GGATGGGTACTTAGGCTCTA+AGG | 0.431894 | 3.4:+94423236 | MS.gene073029:CDS |
| TTTCTTTGGTAAGAGCTGAT+AGG | 0.431958 | 3.4:+94423027 | MS.gene073029:CDS |
| AAAAGTTTAAGGATTCTGAT+TGG | 0.433232 | 3.4:+94423174 | MS.gene073029:CDS |
| TTATTTCCAGCAACTTGTAA+TGG | 0.434209 | 3.4:-94422734 | None:intergenic |
| GCTGCACAATTTCAAAAGAA+AGG | 0.440288 | 3.4:+94422705 | MS.gene073029:CDS |
| GACATTTCAGATGATTCCTA+TGG | 0.446146 | 3.4:+94423140 | MS.gene073029:CDS |
| CAATACATCAACAATGGGAA+TGG | 0.467040 | 3.4:+94422846 | MS.gene073029:CDS |
| AAGTGTGTGGCTGCTCTCGA+TGG | 0.472530 | 3.4:+94423110 | MS.gene073029:CDS |
| CATCCACGCTTCCTCTCATT+AGG | 0.478275 | 3.4:-94423218 | None:intergenic |
| TTTCGTCATCTGTGTTTGGT+GGG | 0.480692 | 3.4:-94422809 | None:intergenic |
| CTGATAGGTTAAGAAGGGAT+AGG | 0.484536 | 3.4:+94423042 | MS.gene073029:CDS |
| ATGATCAATACATCAACAAT+GGG | 0.488653 | 3.4:+94422841 | MS.gene073029:CDS |
| TAATCATGTGCATGTTGATA+AGG | 0.492675 | 3.4:+94422875 | MS.gene073029:CDS |
| GTTTAAGGATTCTGATTGGA+GGG | 0.496068 | 3.4:+94423178 | MS.gene073029:CDS |
| GAATGAGGCACTTGCAGCAT+GGG | 0.501641 | 3.4:+94422998 | MS.gene073029:CDS |
| TAAGAGCTGATAGGTTAAGA+AGG | 0.504282 | 3.4:+94423036 | MS.gene073029:CDS |
| CTGCACAATTTCAAAAGAAA+GGG | 0.506707 | 3.4:+94422706 | MS.gene073029:CDS |
| AATACATCAACAATGGGAAT+GGG | 0.512612 | 3.4:+94422847 | MS.gene073029:CDS |
| AAGAGCTGATAGGTTAAGAA+GGG | 0.537119 | 3.4:+94423037 | MS.gene073029:CDS |
| GGAAGCGTGGATGGGTACTT+AGG | 0.537157 | 3.4:+94423228 | MS.gene073029:CDS |
| CTAATGAGAGGAAGCGTGGA+TGG | 0.541911 | 3.4:+94423219 | MS.gene073029:CDS |
| GATGATCAATACATCAACAA+TGG | 0.551531 | 3.4:+94422840 | MS.gene073029:CDS |
| CACTCGTAGTGAAAAGCGAC+AGG | 0.562107 | 3.4:+94422935 | MS.gene073029:CDS |
| AGTTTAAGGATTCTGATTGG+AGG | 0.570217 | 3.4:+94423177 | MS.gene073029:CDS |
| ATCGAGAGCAGCCACACACT+TGG | 0.570899 | 3.4:-94423108 | None:intergenic |
| AGTGTGTGGCTGCTCTCGAT+GGG | 0.579630 | 3.4:+94423111 | MS.gene073029:CDS |
| TGATTCCTATGGAAAAGCGT+TGG | 0.584496 | 3.4:+94423151 | MS.gene073029:CDS |
| TACATCACATCATATGAATG+AGG | 0.591772 | 3.4:+94422983 | MS.gene073029:CDS |
| TAATGAGAGGAAGCGTGGAT+GGG | 0.591816 | 3.4:+94423220 | MS.gene073029:CDS |
| TGAATGAGGCACTTGCAGCA+TGG | 0.593350 | 3.4:+94422997 | MS.gene073029:CDS |
| TTGTTCTCTTACCAAGTGTG+TGG | 0.604130 | 3.4:+94423097 | MS.gene073029:CDS |
| GGTTCATGTGAGATCCAAGA+AGG | 0.621915 | 3.4:+94422956 | MS.gene073029:CDS |
| ATGCCTAATGAGAGGAAGCG+TGG | 0.638032 | 3.4:+94423215 | MS.gene073029:CDS |
| AAGAAGGGATAGGAGTGCAG+AGG | 0.647414 | 3.4:+94423052 | MS.gene073029:CDS |
| TTATCGCAATGCCTAATGAG+AGG | 0.665091 | 3.4:+94423207 | MS.gene073029:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAGTTTAAGGATTCTGAT+TGG | + | chr3.4:94423174-94423193 | MS.gene073029:CDS | 25.0% |
| ! | ATGATCAATACATCAACAAT+GGG | + | chr3.4:94422841-94422860 | MS.gene073029:CDS | 25.0% |
| AATACATCAACAATGGGAAT+GGG | + | chr3.4:94422847-94422866 | MS.gene073029:CDS | 30.0% | |
| CTGCACAATTTCAAAAGAAA+GGG | + | chr3.4:94422706-94422725 | MS.gene073029:CDS | 30.0% | |
| GATGATCAATACATCAACAA+TGG | + | chr3.4:94422840-94422859 | MS.gene073029:CDS | 30.0% | |
| TAATCATGTGCATGTTGATA+AGG | + | chr3.4:94422875-94422894 | MS.gene073029:CDS | 30.0% | |
| TACATCACATCATATGAATG+AGG | + | chr3.4:94422983-94423002 | MS.gene073029:CDS | 30.0% | |
| TTATTTCCAGCAACTTGTAA+TGG | - | chr3.4:94422737-94422756 | None:intergenic | 30.0% | |
| TTCAACAAAAACAATGCAAC+TGG | + | chr3.4:94422759-94422778 | MS.gene073029:CDS | 30.0% | |
| !! | AAAAGCGTTGGAAAAGTTTA+AGG | + | chr3.4:94423163-94423182 | MS.gene073029:CDS | 30.0% |
| !!! | TTTTGAAATTGTGCAGCTTT+GGG | - | chr3.4:94422702-94422721 | None:intergenic | 30.0% |
| AAGAGCTGATAGGTTAAGAA+GGG | + | chr3.4:94423037-94423056 | MS.gene073029:CDS | 35.0% | |
| AGTTTAAGGATTCTGATTGG+AGG | + | chr3.4:94423177-94423196 | MS.gene073029:CDS | 35.0% | |
| CAATACATCAACAATGGGAA+TGG | + | chr3.4:94422846-94422865 | MS.gene073029:CDS | 35.0% | |
| GACATTTCAGATGATTCCTA+TGG | + | chr3.4:94423140-94423159 | MS.gene073029:CDS | 35.0% | |
| GCTGCACAATTTCAAAAGAA+AGG | + | chr3.4:94422705-94422724 | MS.gene073029:CDS | 35.0% | |
| GTTTAAGGATTCTGATTGGA+GGG | + | chr3.4:94423178-94423197 | MS.gene073029:CDS | 35.0% | |
| TAAGAGCTGATAGGTTAAGA+AGG | + | chr3.4:94423036-94423055 | MS.gene073029:CDS | 35.0% | |
| TTTCTTTGGTAAGAGCTGAT+AGG | + | chr3.4:94423027-94423046 | MS.gene073029:CDS | 35.0% | |
| !!! | CTTTTGAAATTGTGCAGCTT+TGG | - | chr3.4:94422703-94422722 | None:intergenic | 35.0% |
| ATGTGATGTAGACTCCTTCT+TGG | - | chr3.4:94422973-94422992 | None:intergenic | 40.0% | |
| CTGATAGGTTAAGAAGGGAT+AGG | + | chr3.4:94423042-94423061 | MS.gene073029:CDS | 40.0% | |
| TTATCGCAATGCCTAATGAG+AGG | + | chr3.4:94423207-94423226 | MS.gene073029:CDS | 40.0% | |
| TTGTTCTCTTACCAAGTGTG+TGG | + | chr3.4:94423097-94423116 | MS.gene073029:CDS | 40.0% | |
| TTTCGTCATCTGTGTTTGGT+GGG | - | chr3.4:94422812-94422831 | None:intergenic | 40.0% | |
| ! | CTTTTCCAACGCTTTTCCAT+AGG | - | chr3.4:94423159-94423178 | None:intergenic | 40.0% |
| ! | TCGTTTTCGTCATCTGTGTT+TGG | - | chr3.4:94422816-94422835 | None:intergenic | 40.0% |
| ! | TGATTCCTATGGAAAAGCGT+TGG | + | chr3.4:94423151-94423170 | MS.gene073029:CDS | 40.0% |
| ! | TTTTCGTCATCTGTGTTTGG+TGG | - | chr3.4:94422813-94422832 | None:intergenic | 40.0% |
| AGCATGGGCTCAAGTTTCTT+TGG | + | chr3.4:94423013-94423032 | MS.gene073029:CDS | 45.0% | |
| GGTTCATGTGAGATCCAAGA+AGG | + | chr3.4:94422956-94422975 | MS.gene073029:CDS | 45.0% | |
| GTGTGACCATTACAAGTTGC+TGG | + | chr3.4:94422728-94422747 | MS.gene073029:CDS | 45.0% | |
| ! | ATCTGTGTTTGGTGGGTCTT+GGG | - | chr3.4:94422805-94422824 | None:intergenic | 45.0% |
| ! | TAATGAGAGGAAGCGTGGAT+GGG | + | chr3.4:94423220-94423239 | MS.gene073029:CDS | 45.0% |
| ATGCCTAATGAGAGGAAGCG+TGG | + | chr3.4:94423215-94423234 | MS.gene073029:CDS | 50.0% | |
| CACTCGTAGTGAAAAGCGAC+AGG | + | chr3.4:94422935-94422954 | MS.gene073029:CDS | 50.0% | |
| CATCCACGCTTCCTCTCATT+AGG | - | chr3.4:94423221-94423240 | None:intergenic | 50.0% | |
| ! | AAGAAGGGATAGGAGTGCAG+AGG | + | chr3.4:94423052-94423071 | MS.gene073029:CDS | 50.0% |
| ! | CATCTGTGTTTGGTGGGTCT+TGG | - | chr3.4:94422806-94422825 | None:intergenic | 50.0% |
| ! | CTAATGAGAGGAAGCGTGGA+TGG | + | chr3.4:94423219-94423238 | MS.gene073029:CDS | 50.0% |
| ! | GAATGAGGCACTTGCAGCAT+GGG | + | chr3.4:94422998-94423017 | MS.gene073029:CDS | 50.0% |
| ! | TGAATGAGGCACTTGCAGCA+TGG | + | chr3.4:94422997-94423016 | MS.gene073029:CDS | 50.0% |
| AAGTGTGTGGCTGCTCTCGA+TGG | + | chr3.4:94423110-94423129 | MS.gene073029:CDS | 55.0% | |
| AGTGTGTGGCTGCTCTCGAT+GGG | + | chr3.4:94423111-94423130 | MS.gene073029:CDS | 55.0% | |
| ATCGAGAGCAGCCACACACT+TGG | - | chr3.4:94423111-94423130 | None:intergenic | 55.0% | |
| ! | GGAAGCGTGGATGGGTACTT+AGG | + | chr3.4:94423228-94423247 | MS.gene073029:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 94422675 | 94423256 | 94422675 | ID=MS.gene073029 |
| chr3.4 | mRNA | 94422675 | 94423256 | 94422675 | ID=MS.gene073029.t1;Parent=MS.gene073029 |
| chr3.4 | exon | 94422675 | 94423256 | 94422675 | ID=MS.gene073029.t1.exon1;Parent=MS.gene073029.t1 |
| chr3.4 | CDS | 94422675 | 94423256 | 94422675 | ID=cds.MS.gene073029.t1;Parent=MS.gene073029.t1 |
| Gene Sequence |
| Protein sequence |
