Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073579.t1 | XP_013467320.1 | 98 | 406 | 7 | 1 | 1 | 405 | 1 | 406 | 4.20E-219 | 770.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073579.t1 | Q03441 | 22.5 | 222 | 163 | 2 | 182 | 398 | 205 | 422 | 1.1e-10 | 69.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073579.t1 | A0A072VHW2 | 98.0 | 406 | 7 | 1 | 1 | 405 | 1 | 406 | 3.0e-219 | 770.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050060 | MS.gene073579 | 0.800303 | 1.54E-48 | -1.69E-46 |
| MS.gene050426 | MS.gene073579 | -0.824449 | 8.02E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene073579 | -0.804768 | 1.84E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene073579 | 0.859009 | 5.56E-63 | -1.69E-46 |
| MS.gene051983 | MS.gene073579 | 0.845761 | 3.32E-59 | -1.69E-46 |
| MS.gene052011 | MS.gene073579 | -0.800558 | 1.36E-48 | -1.69E-46 |
| MS.gene052077 | MS.gene073579 | 0.813251 | 2.81E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene073579 | 0.826054 | 3.35E-54 | -1.69E-46 |
| MS.gene054349 | MS.gene073579 | 0.803259 | 3.80E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene073579 | 0.828635 | 8.06E-55 | -1.69E-46 |
| MS.gene056979 | MS.gene073579 | 0.834755 | 2.49E-56 | -1.69E-46 |
| MS.gene05811 | MS.gene073579 | 0.81543 | 9.28E-52 | -1.69E-46 |
| MS.gene058142 | MS.gene073579 | 0.802587 | 5.23E-49 | -1.69E-46 |
| MS.gene058276 | MS.gene073579 | 0.805838 | 1.10E-49 | -1.69E-46 |
| MS.gene058743 | MS.gene073579 | 0.802247 | 6.14E-49 | -1.69E-46 |
| MS.gene059788 | MS.gene073579 | 0.806443 | 8.21E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene073579 | 0.831337 | 1.77E-55 | -1.69E-46 |
| MS.gene060083 | MS.gene073579 | 0.810504 | 1.12E-50 | -1.69E-46 |
| MS.gene060717 | MS.gene073579 | 0.819983 | 8.72E-53 | -1.69E-46 |
| MS.gene060986 | MS.gene073579 | 0.807801 | 4.23E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073579.t1 | MTR_1g049180 | 98.030 | 406 | 7 | 1 | 1 | 405 | 1 | 406 | 0.0 | 817 |
| MS.gene073579.t1 | MTR_5g043180 | 59.561 | 319 | 120 | 5 | 87 | 401 | 95 | 408 | 6.22e-128 | 375 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073579.t1 | AT5G13610 | 74.048 | 289 | 75 | 0 | 113 | 401 | 113 | 401 | 1.96e-157 | 449 |
| MS.gene073579.t1 | AT1G69380 | 59.044 | 293 | 119 | 1 | 111 | 402 | 77 | 369 | 8.16e-122 | 358 |
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGTGAGATTGTGATTT+TGG | 0.142702 | 1.1:-34082537 | None:intergenic |
| TCACCATCCTTTCTCATTTC+AGG | 0.181892 | 1.1:-34086386 | None:intergenic |
| CTCCCATATCTGACCATATT+TGG | 0.221053 | 1.1:-34092364 | None:intergenic |
| CCTGGTTCTTCTTTCTTGAA+TGG | 0.268839 | 1.1:+34086245 | MS.gene073579:CDS |
| ACCATATTTGGCATCTTTCC+AGG | 0.288418 | 1.1:-34092352 | None:intergenic |
| GCTGATAATGTTAATGATAA+TGG | 0.306101 | 1.1:+34082705 | MS.gene073579:CDS |
| CCACTTGTATTCTAGGTTGA+TGG | 0.321901 | 1.1:+34091186 | MS.gene073579:intron |
| CGTCGTTTCCTGCACTTGAT+TGG | 0.326621 | 1.1:+34082646 | MS.gene073579:CDS |
| AATTATGTAGTTCTCAAGTT+TGG | 0.333870 | 1.1:+34085176 | MS.gene073579:CDS |
| GTTGATGGAATTCGAACTAT+TGG | 0.339072 | 1.1:+34091037 | MS.gene073579:CDS |
| TACAAGAAATTCTTCAAAAC+AGG | 0.343403 | 1.1:+34092581 | MS.gene073579:CDS |
| ATGCCAAATATGGTCAGATA+TGG | 0.351572 | 1.1:+34092361 | MS.gene073579:CDS |
| AGTATTGCTCTTGATTACTA+TGG | 0.353838 | 1.1:+34091076 | MS.gene073579:CDS |
| ATGGCTATTTGAAAATTGTT+AGG | 0.362493 | 1.1:+34086342 | MS.gene073579:CDS |
| GTTCTCAAGTTTGGCAATCT+TGG | 0.366939 | 1.1:+34085185 | MS.gene073579:CDS |
| GATGATGGTGTGACAAAGTT+TGG | 0.373642 | 1.1:-34085143 | None:intergenic |
| CGAACTATTGGTAGTGTACT+AGG | 0.382786 | 1.1:+34091049 | MS.gene073579:CDS |
| GTCAGATATGGGAGTATCTC+AGG | 0.391860 | 1.1:+34092373 | MS.gene073579:CDS |
| GCCTGGAAAGATGCCAAATA+TGG | 0.392620 | 1.1:+34092351 | MS.gene073579:CDS |
| AACGCTCTAACAGGAATTGC+AGG | 0.396685 | 1.1:-34082750 | None:intergenic |
| TGCATCCATGTGCTCAAAGC+TGG | 0.404184 | 1.1:-34090977 | None:intergenic |
| TTACATAATGTTACAATACT+TGG | 0.404564 | 1.1:+34091012 | MS.gene073579:CDS |
| AATTTGCCTTGCCCACCAAC+TGG | 0.414032 | 1.1:-34091287 | None:intergenic |
| TGGCTCATTATTGCATTGAT+TGG | 0.416590 | 1.1:+34092622 | MS.gene073579:CDS |
| TTACAATACTTGGATGTTGA+TGG | 0.418643 | 1.1:+34091022 | MS.gene073579:CDS |
| GAGAAGAAGAACGCTCTAAC+AGG | 0.420264 | 1.1:-34082759 | None:intergenic |
| TCTTTCTTGAATGGAACAAA+TGG | 0.422190 | 1.1:+34086254 | MS.gene073579:CDS |
| CACATGGATGCAAGGAGGAC+TGG | 0.427102 | 1.1:+34090988 | MS.gene073579:CDS |
| TGATTTCAAGTTGAAATTTG+TGG | 0.429980 | 1.1:+34092431 | MS.gene073579:CDS |
| GAAGAATAAGAAGAAGTGTT+TGG | 0.436254 | 1.1:-34082480 | None:intergenic |
| ATTGTTAGGAAGCATGCATC+TGG | 0.438001 | 1.1:+34086356 | MS.gene073579:CDS |
| TGATAATGGAACAATTGATC+AGG | 0.456167 | 1.1:+34082719 | MS.gene073579:CDS |
| ATGAAAACGACGAGTGATGA+AGG | 0.462074 | 1.1:-34082589 | None:intergenic |
| GTCCTCGAACATGAGGTTGA+TGG | 0.502037 | 1.1:+34086323 | MS.gene073579:CDS |
| AGAAGTGTTTGGTGAAGGAG+AGG | 0.508031 | 1.1:-34082469 | None:intergenic |
| TTGGTGAAGGAGAGGAGAAG+AGG | 0.509293 | 1.1:-34082461 | None:intergenic |
| TTTGCTTCCTGAAATGAGAA+AGG | 0.519357 | 1.1:+34086379 | MS.gene073579:CDS |
| GAGCGTTCTTCTTCTCCACC+AGG | 0.523899 | 1.1:+34082766 | MS.gene073579:CDS |
| TAATTAGTCATCCTTGATGA+TGG | 0.530000 | 1.1:-34085158 | None:intergenic |
| GAGGAAAGAACGAAGGAGTG+AGG | 0.532384 | 1.1:-34082442 | None:intergenic |
| TAAGAAGAAGTGTTTGGTGA+AGG | 0.533777 | 1.1:-34082474 | None:intergenic |
| TTTACAGATCAGATATCGCC+TGG | 0.534883 | 1.1:+34092334 | MS.gene073579:intron |
| AGCCATCAACCTCATGTTCG+AGG | 0.536713 | 1.1:-34086325 | None:intergenic |
| TGCCAAATATGGTCAGATAT+GGG | 0.539187 | 1.1:+34092362 | MS.gene073579:CDS |
| AGGAGAAGAGGAAAGAACGA+AGG | 0.547618 | 1.1:-34082449 | None:intergenic |
| TTGCTCTTGATTACTATGGC+CGG | 0.547854 | 1.1:+34091080 | MS.gene073579:CDS |
| TGGCAATCTTGGCGATTCCA+AGG | 0.548774 | 1.1:+34085196 | MS.gene073579:CDS |
| CTGGAAAATTTAAGATGCAA+AGG | 0.558742 | 1.1:+34091253 | MS.gene073579:CDS |
| ATCAACCTCAGAGCATGAAA+CGG | 0.560927 | 1.1:-34082676 | None:intergenic |
| GCGTCGTTCCAATCAAGTGC+AGG | 0.566378 | 1.1:-34082654 | None:intergenic |
| TGTATTCTAGGTTGATGGAA+TGG | 0.567013 | 1.1:+34091191 | MS.gene073579:intron |
| CTCTATATGACATTGTTCAA+AGG | 0.569742 | 1.1:+34092662 | MS.gene073579:CDS |
| GCTTTGAGCACATGGATGCA+AGG | 0.583404 | 1.1:+34090980 | MS.gene073579:CDS |
| GTTTAATGTCCTCGAACATG+AGG | 0.583906 | 1.1:+34086316 | MS.gene073579:CDS |
| AAGGAGTGAGGTGGTTCTAA+CGG | 0.585572 | 1.1:-34082430 | None:intergenic |
| TCTTGATTACTATGGCCGGC+AGG | 0.586842 | 1.1:+34091084 | MS.gene073579:CDS |
| AATCGTGAGATGGAAGCGAC+TGG | 0.588881 | 1.1:+34091234 | MS.gene073579:CDS |
| GGAAATCTGATTTCCTTGAG+TGG | 0.592677 | 1.1:+34092602 | MS.gene073579:CDS |
| ATGCAATAATGAGCCACTCA+AGG | 0.594797 | 1.1:-34092615 | None:intergenic |
| GCTGATGTGATTCTAAAGCT+CGG | 0.595619 | 1.1:+34091318 | MS.gene073579:CDS |
| ACTTTGTCACACCATCATCA+AGG | 0.597279 | 1.1:+34085147 | MS.gene073579:CDS |
| TTTCAAGTTGAAATTTGTGG+AGG | 0.601208 | 1.1:+34092434 | MS.gene073579:CDS |
| TACAGACATAAATCGTGAGA+TGG | 0.607409 | 1.1:+34091224 | MS.gene073579:CDS |
| TGGAACAAATGGCTGTTACA+TGG | 0.615782 | 1.1:+34086265 | MS.gene073579:CDS |
| CTTCCTGAAATGAGAAAGGA+TGG | 0.617434 | 1.1:+34086383 | MS.gene073579:CDS |
| TAAAGCTCGGACTATTTGAG+AGG | 0.622265 | 1.1:+34091331 | MS.gene073579:CDS |
| CCATTCAAGAAAGAAGAACC+AGG | 0.624252 | 1.1:-34086245 | None:intergenic |
| CGACGCCGTTTCATGCTCTG+AGG | 0.628847 | 1.1:+34082671 | MS.gene073579:CDS |
| GAGGACATTAAACAATACAA+TGG | 0.634285 | 1.1:-34086306 | None:intergenic |
| TCAGATATGGGAGTATCTCA+GGG | 0.654645 | 1.1:+34092374 | MS.gene073579:CDS |
| AGGTGGTTCTAACGGTGAGG+TGG | 0.657935 | 1.1:-34082422 | None:intergenic |
| GAAAGAACGAAGGAGTGAGG+TGG | 0.660458 | 1.1:-34082439 | None:intergenic |
| GAAACGACGATGACGAAACG+CGG | 0.668811 | 1.1:-34082632 | None:intergenic |
| TCTAACGGTGAGGTGGTGTG+TGG | 0.673751 | 1.1:-34082415 | None:intergenic |
| GTGAGGTGGTTCTAACGGTG+AGG | 0.674620 | 1.1:-34082425 | None:intergenic |
| TTGAGCACATGGATGCAAGG+AGG | 0.721239 | 1.1:+34090983 | MS.gene073579:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 34082396 | 34092702 | 34082396 | ID=MS.gene073579 |
| chr1.1 | mRNA | 34082396 | 34092702 | 34082396 | ID=MS.gene073579.t1;Parent=MS.gene073579 |
| chr1.1 | exon | 34082396 | 34082787 | 34082396 | ID=MS.gene073579.t1.exon1;Parent=MS.gene073579.t1 |
| chr1.1 | CDS | 34082396 | 34082787 | 34082396 | ID=cds.MS.gene073579.t1;Parent=MS.gene073579.t1 |
| chr1.1 | exon | 34085109 | 34085217 | 34085109 | ID=MS.gene073579.t1.exon2;Parent=MS.gene073579.t1 |
| chr1.1 | CDS | 34085109 | 34085217 | 34085109 | ID=cds.MS.gene073579.t1;Parent=MS.gene073579.t1 |
| chr1.1 | exon | 34086242 | 34086404 | 34086242 | ID=MS.gene073579.t1.exon3;Parent=MS.gene073579.t1 |
| chr1.1 | CDS | 34086242 | 34086404 | 34086242 | ID=cds.MS.gene073579.t1;Parent=MS.gene073579.t1 |
| chr1.1 | exon | 34090957 | 34091105 | 34090957 | ID=MS.gene073579.t1.exon4;Parent=MS.gene073579.t1 |
| chr1.1 | CDS | 34090957 | 34091105 | 34090957 | ID=cds.MS.gene073579.t1;Parent=MS.gene073579.t1 |
| chr1.1 | exon | 34091201 | 34091352 | 34091201 | ID=MS.gene073579.t1.exon5;Parent=MS.gene073579.t1 |
| chr1.1 | CDS | 34091201 | 34091352 | 34091201 | ID=cds.MS.gene073579.t1;Parent=MS.gene073579.t1 |
| chr1.1 | exon | 34092341 | 34092455 | 34092341 | ID=MS.gene073579.t1.exon6;Parent=MS.gene073579.t1 |
| chr1.1 | CDS | 34092341 | 34092455 | 34092341 | ID=cds.MS.gene073579.t1;Parent=MS.gene073579.t1 |
| chr1.1 | exon | 34092565 | 34092702 | 34092565 | ID=MS.gene073579.t1.exon7;Parent=MS.gene073579.t1 |
| chr1.1 | CDS | 34092565 | 34092702 | 34092565 | ID=cds.MS.gene073579.t1;Parent=MS.gene073579.t1 |
| Gene Sequence |
| Protein sequence |
