Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08724.t1 | XP_013456005.1 | 99.1 | 107 | 1 | 0 | 1 | 107 | 79 | 185 | 9.80E-51 | 209.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08724.t1 | Q9SKN3 | 77.6 | 107 | 24 | 0 | 1 | 107 | 73 | 179 | 2.7e-41 | 169.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08724.t1 | I3SCI9 | 99.1 | 107 | 1 | 0 | 1 | 107 | 79 | 185 | 7.1e-51 | 209.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049653 | MS.gene08724 | 0.805954 | 1.04E-49 | -1.69E-46 |
| MS.gene050437 | MS.gene08724 | 0.816095 | 6.60E-52 | -1.69E-46 |
| MS.gene050689 | MS.gene08724 | 0.831283 | 1.82E-55 | -1.69E-46 |
| MS.gene050752 | MS.gene08724 | 0.839662 | 1.39E-57 | -1.69E-46 |
| MS.gene050955 | MS.gene08724 | 0.823536 | 1.31E-53 | -1.69E-46 |
| MS.gene051003 | MS.gene08724 | 0.824071 | 9.84E-54 | -1.69E-46 |
| MS.gene051234 | MS.gene08724 | 0.834956 | 2.22E-56 | -1.69E-46 |
| MS.gene05134 | MS.gene08724 | 0.80324 | 3.83E-49 | -1.69E-46 |
| MS.gene051365 | MS.gene08724 | 0.815995 | 6.95E-52 | -1.69E-46 |
| MS.gene051856 | MS.gene08724 | 0.810763 | 9.80E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene08724 | 0.806497 | 7.99E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene08724 | 0.803921 | 2.77E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene08724 | 0.835238 | 1.89E-56 | -1.69E-46 |
| MS.gene05364 | MS.gene08724 | 0.823533 | 1.32E-53 | -1.69E-46 |
| MS.gene053678 | MS.gene08724 | 0.819081 | 1.40E-52 | -1.69E-46 |
| MS.gene054250 | MS.gene08724 | 0.814036 | 1.89E-51 | -1.69E-46 |
| MS.gene054403 | MS.gene08724 | 0.839029 | 2.02E-57 | -1.69E-46 |
| MS.gene055513 | MS.gene08724 | 0.801927 | 7.15E-49 | -1.69E-46 |
| MS.gene057102 | MS.gene08724 | 0.825273 | 5.13E-54 | -1.69E-46 |
| MS.gene057208 | MS.gene08724 | 0.800198 | 1.61E-48 | -1.69E-46 |
| MS.gene057705 | MS.gene08724 | 0.808039 | 3.77E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene08724 | 0.803692 | 3.09E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene08724 | 0.801878 | 7.32E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene08724 | 0.814441 | 1.54E-51 | -1.69E-46 |
| MS.gene060937 | MS.gene08724 | 0.800255 | 1.57E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTGATGTTTATGCCATTT+TGG | 0.142401 | 4.4:-44872520 | MS.gene08724:CDS |
| TCTTCTTAACTTCTGGTCTT+TGG | 0.220309 | 4.4:-44872112 | MS.gene08724:CDS |
| CGTCATTTCTTCTTAACTTC+TGG | 0.294825 | 4.4:-44872119 | MS.gene08724:CDS |
| CTGCTGGCTTGCAGACTTTG+TGG | 0.320664 | 4.4:-44872560 | MS.gene08724:CDS |
| CAAAGATCGTCTTACCAAAA+TGG | 0.321266 | 4.4:+44872506 | None:intergenic |
| AGTTTCCTTGTTGCGGCTGC+TGG | 0.344483 | 4.4:-44872576 | MS.gene08724:CDS |
| CTTGTCAGTTCATTTACAAT+TGG | 0.411892 | 4.4:-44872471 | MS.gene08724:CDS |
| GTGCTCATTGATAATGATCT+TGG | 0.426791 | 4.4:-44872219 | MS.gene08724:CDS |
| GCAGCAGCTTGTGCATCAGC+GGG | 0.451898 | 4.4:-44872249 | MS.gene08724:CDS |
| TGCAGCAGCTTGTGCATCAG+CGG | 0.471402 | 4.4:-44872250 | MS.gene08724:CDS |
| CAGGCATGGCATTCATATGC+TGG | 0.517979 | 4.4:-44872155 | MS.gene08724:CDS |
| GTTCATTTACAATTGGCGAT+GGG | 0.519096 | 4.4:-44872464 | MS.gene08724:CDS |
| GTTCAGTTTGAAACGGCTAC+AGG | 0.524200 | 4.4:-44872174 | MS.gene08724:CDS |
| CCACTGTGTTCAGTTTGAAA+CGG | 0.533491 | 4.4:-44872181 | MS.gene08724:CDS |
| AGTTCATTTACAATTGGCGA+TGG | 0.548303 | 4.4:-44872465 | MS.gene08724:CDS |
| TTCATTTACAATTGGCGATG+GGG | 0.560218 | 4.4:-44872463 | MS.gene08724:intron |
| GTTTGAAACGGCTACAGGCA+TGG | 0.571754 | 4.4:-44872169 | MS.gene08724:CDS |
| GCAAGCCAGCAGCCGCAACA+AGG | 0.578048 | 4.4:+44872571 | None:intergenic |
| ACTTCTGGTCTTTGGCATCG+AGG | 0.636800 | 4.4:-44872104 | MS.gene08724:CDS |
| CAGAAGTTAAGAAGAAATGA+CGG | 0.644975 | 4.4:+44872120 | None:intergenic |
| TTGTAAATGAACTGACAAGT+CGG | 0.646171 | 4.4:+44872475 | None:intergenic |
| CCGTTTCAAACTGAACACAG+TGG | 0.767887 | 4.4:+44872181 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTATATAGCATTTTCAAAT+GGG | + | chr4.4:44872350-44872369 | None:intergenic | 20.0% |
| !! | CTCTATATAGCATTTTCAAA+TGG | + | chr4.4:44872351-44872370 | None:intergenic | 25.0% |
| ACATGTATCTTATTACATGC+AGG | - | chr4.4:44872383-44872402 | MS.gene08724:intron | 30.0% | |
| CAGAAGTTAAGAAGAAATGA+CGG | + | chr4.4:44872558-44872577 | None:intergenic | 30.0% | |
| CTTGTCAGTTCATTTACAAT+TGG | - | chr4.4:44872204-44872223 | MS.gene08724:CDS | 30.0% | |
| TTGTAAATGAACTGACAAGT+CGG | + | chr4.4:44872203-44872222 | None:intergenic | 30.0% | |
| ! | ACAATATTAACGAGTCTTGT+TGG | + | chr4.4:44872246-44872265 | None:intergenic | 30.0% |
| !! | TACTGATGTTTATGCCATTT+TGG | - | chr4.4:44872155-44872174 | MS.gene08724:CDS | 30.0% |
| AGTTCATTTACAATTGGCGA+TGG | - | chr4.4:44872210-44872229 | MS.gene08724:CDS | 35.0% | |
| CAAAGATCGTCTTACCAAAA+TGG | + | chr4.4:44872172-44872191 | None:intergenic | 35.0% | |
| CGTCATTTCTTCTTAACTTC+TGG | - | chr4.4:44872556-44872575 | MS.gene08724:CDS | 35.0% | |
| GTTCATTTACAATTGGCGAT+GGG | - | chr4.4:44872211-44872230 | MS.gene08724:CDS | 35.0% | |
| TTCATTTACAATTGGCGATG+GGG | - | chr4.4:44872212-44872231 | MS.gene08724:CDS | 35.0% | |
| ! | TCTTCTTAACTTCTGGTCTT+TGG | - | chr4.4:44872563-44872582 | MS.gene08724:CDS | 35.0% |
| !! | GTGCTCATTGATAATGATCT+TGG | - | chr4.4:44872456-44872475 | MS.gene08724:intron | 35.0% |
| AAGCTTCATAACGTGGACAT+TGG | - | chr4.4:44872297-44872316 | MS.gene08724:intron | 40.0% | |
| ATGTCCACGTTATGAAGCTT+AGG | + | chr4.4:44872297-44872316 | None:intergenic | 40.0% | |
| CCACTGTGTTCAGTTTGAAA+CGG | - | chr4.4:44872494-44872513 | MS.gene08724:CDS | 40.0% | |
| CACTCCTAAGCTTCATAACG+TGG | - | chr4.4:44872290-44872309 | MS.gene08724:intron | 45.0% | |
| CCGTTTCAAACTGAACACAG+TGG | + | chr4.4:44872497-44872516 | None:intergenic | 45.0% | |
| ! | GTTCAGTTTGAAACGGCTAC+AGG | - | chr4.4:44872501-44872520 | MS.gene08724:CDS | 45.0% |
| CAGGCATGGCATTCATATGC+TGG | - | chr4.4:44872520-44872539 | MS.gene08724:CDS | 50.0% | |
| ! | ACTTCTGGTCTTTGGCATCG+AGG | - | chr4.4:44872571-44872590 | MS.gene08724:CDS | 50.0% |
| ! | GTTTGAAACGGCTACAGGCA+TGG | - | chr4.4:44872506-44872525 | MS.gene08724:CDS | 50.0% |
| TGCAGCAGCTTGTGCATCAG+CGG | - | chr4.4:44872425-44872444 | MS.gene08724:intron | 55.0% | |
| !! | CTGCTGGCTTGCAGACTTTG+TGG | - | chr4.4:44872115-44872134 | MS.gene08724:CDS | 55.0% |
| GCAGCAGCTTGTGCATCAGC+GGG | - | chr4.4:44872426-44872445 | MS.gene08724:intron | 60.0% | |
| GCAAGCCAGCAGCCGCAACA+AGG | + | chr4.4:44872107-44872126 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 44872101 | 44872596 | 44872101 | ID=MS.gene08724 |
| chr4.4 | mRNA | 44872101 | 44872596 | 44872101 | ID=MS.gene08724.t1;Parent=MS.gene08724 |
| chr4.4 | exon | 44872464 | 44872596 | 44872464 | ID=MS.gene08724.t1.exon1;Parent=MS.gene08724.t1 |
| chr4.4 | CDS | 44872464 | 44872596 | 44872464 | ID=cds.MS.gene08724.t1;Parent=MS.gene08724.t1 |
| chr4.4 | exon | 44872101 | 44872292 | 44872101 | ID=MS.gene08724.t1.exon2;Parent=MS.gene08724.t1 |
| chr4.4 | CDS | 44872101 | 44872292 | 44872101 | ID=cds.MS.gene08724.t1;Parent=MS.gene08724.t1 |
| Gene Sequence |
| Protein sequence |
