Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09138.t1 | XP_024637929.1 | 98.6 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 1.10E-29 | 138.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09138.t1 | G7JM87 | 98.6 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 7.6e-30 | 138.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene09138 | 0.814961 | 1.18E-51 | -1.69E-46 |
| MS.gene049341 | MS.gene09138 | 0.805769 | 1.14E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene09138 | 0.802254 | 6.12E-49 | -1.69E-46 |
| MS.gene050442 | MS.gene09138 | 0.800696 | 1.28E-48 | -1.69E-46 |
| MS.gene050724 | MS.gene09138 | 0.801222 | 9.97E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene09138 | 0.813731 | 2.21E-51 | -1.69E-46 |
| MS.gene050815 | MS.gene09138 | 0.809556 | 1.78E-50 | -1.69E-46 |
| MS.gene051243 | MS.gene09138 | 0.824264 | 8.87E-54 | -1.69E-46 |
| MS.gene051328 | MS.gene09138 | 0.807559 | 4.76E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene09138 | 0.81787 | 2.63E-52 | -1.69E-46 |
| MS.gene054472 | MS.gene09138 | 0.805303 | 1.42E-49 | -1.69E-46 |
| MS.gene055512 | MS.gene09138 | 0.806171 | 9.36E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene09138 | 0.825144 | 5.50E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene09138 | 0.808396 | 3.16E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene09138 | 0.817972 | 2.50E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene09138 | 0.803382 | 3.58E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene09138 | 0.823014 | 1.74E-53 | -1.69E-46 |
| MS.gene057915 | MS.gene09138 | 0.803522 | 3.35E-49 | -1.69E-46 |
| MS.gene058146 | MS.gene09138 | 0.803617 | 3.20E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene09138 | 0.815504 | 8.94E-52 | -1.69E-46 |
| MS.gene059842 | MS.gene09138 | 0.809657 | 1.70E-50 | -1.69E-46 |
| MS.gene059937 | MS.gene09138 | 0.801668 | 8.08E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene09138 | 0.800343 | 1.51E-48 | -1.69E-46 |
| MS.gene060322 | MS.gene09138 | 0.802167 | 6.38E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene09138 | 0.800095 | 1.69E-48 | -1.69E-46 |
| MS.gene060803 | MS.gene09138 | 0.844662 | 6.58E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09138.t1 | MTR_4g057190 | 98.551 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 8.07e-46 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09138.t1 | AT5G22875 | 81.159 | 69 | 13 | 0 | 1 | 69 | 1 | 69 | 3.36e-37 | 119 |
| MS.gene09138.t1 | AT5G22875 | 81.159 | 69 | 13 | 0 | 1 | 69 | 1 | 69 | 3.36e-37 | 119 |
| MS.gene09138.t1 | AT5G22875 | 81.159 | 69 | 13 | 0 | 1 | 69 | 1 | 69 | 3.36e-37 | 119 |
Find 12 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGGACCTTATATTAATAC+TGG | 0.230807 | 4.1:-41057284 | MS.gene09138:CDS |
| GGCTTTGTTGGATCAATTAC+CGG | 0.331164 | 4.1:+41057329 | None:intergenic |
| TTCTTATAAGTTTCATGATC+AGG | 0.354887 | 4.1:+41057245 | None:intergenic |
| TGTGGTGAAAGTGGCTTTGT+TGG | 0.403611 | 4.1:+41057317 | None:intergenic |
| AATACTGGGTCACGTGATAT+TGG | 0.406093 | 4.1:-41057269 | MS.gene09138:CDS |
| CGTGACCCAGTATTAATATA+AGG | 0.410120 | 4.1:+41057278 | None:intergenic |
| TCACCACAAGCTACATTTCG+AGG | 0.438997 | 4.1:-41057302 | MS.gene09138:CDS |
| CGAAATTTAGTAGTTGCTGC+TGG | 0.448263 | 4.1:-41057508 | MS.gene09138:CDS |
| TTAACTTACGCCATGTAGAA+AGG | 0.474118 | 4.1:+41057451 | None:intergenic |
| GAGGACCTTATATTAATACT+GGG | 0.603505 | 4.1:-41057283 | MS.gene09138:CDS |
| AATGTAGCTTGTGGTGAAAG+TGG | 0.617393 | 4.1:+41057308 | None:intergenic |
| GGTCCTCGAAATGTAGCTTG+TGG | 0.650550 | 4.1:+41057299 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTGATAATTTTCATTTT+TGG | - | chr4.1:41057384-41057403 | MS.gene09138:intron | 10.0% |
| !!! | TTTTGATAATTTTCATTTTT+GGG | - | chr4.1:41057385-41057404 | MS.gene09138:intron | 10.0% |
| ! | TTCTTATAAGTTTCATGATC+AGG | + | chr4.1:41057516-41057535 | None:intergenic | 25.0% |
| GAGGACCTTATATTAATACT+GGG | - | chr4.1:41057475-41057494 | MS.gene09138:CDS | 30.0% | |
| CGAGGACCTTATATTAATAC+TGG | - | chr4.1:41057474-41057493 | MS.gene09138:CDS | 35.0% | |
| CGTGACCCAGTATTAATATA+AGG | + | chr4.1:41057483-41057502 | None:intergenic | 35.0% | |
| TTAACTTACGCCATGTAGAA+AGG | + | chr4.1:41057310-41057329 | None:intergenic | 35.0% | |
| ! | AATGGCTTTTCCTTTCTACA+TGG | - | chr4.1:41057297-41057316 | MS.gene09138:CDS | 35.0% |
| !!! | TTTTAGTTCGTCGAAGAAAC+CGG | - | chr4.1:41057410-41057429 | MS.gene09138:intron | 35.0% |
| AATACTGGGTCACGTGATAT+TGG | - | chr4.1:41057489-41057508 | MS.gene09138:CDS | 40.0% | |
| CGAAATTTAGTAGTTGCTGC+TGG | - | chr4.1:41057250-41057269 | MS.gene09138:CDS | 40.0% | |
| ! | AATGTAGCTTGTGGTGAAAG+TGG | + | chr4.1:41057453-41057472 | None:intergenic | 40.0% |
| ! | TTACATGCTTTTGCTTCAGC+AGG | - | chr4.1:41057274-41057293 | MS.gene09138:CDS | 40.0% |
| !! | GGCTTTGTTGGATCAATTAC+CGG | + | chr4.1:41057432-41057451 | None:intergenic | 40.0% |
| TCACCACAAGCTACATTTCG+AGG | - | chr4.1:41057456-41057475 | MS.gene09138:intron | 45.0% | |
| ! | TGCTTTTGCTTCAGCAGGAA+TGG | - | chr4.1:41057279-41057298 | MS.gene09138:CDS | 45.0% |
| !! | TGTGGTGAAAGTGGCTTTGT+TGG | + | chr4.1:41057444-41057463 | None:intergenic | 45.0% |
| GGTCCTCGAAATGTAGCTTG+TGG | + | chr4.1:41057462-41057481 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 41057238 | 41057542 | 41057238 | ID=MS.gene09138 |
| chr4.1 | mRNA | 41057238 | 41057542 | 41057238 | ID=MS.gene09138.t1;Parent=MS.gene09138 |
| chr4.1 | exon | 41057460 | 41057542 | 41057460 | ID=MS.gene09138.t1.exon1;Parent=MS.gene09138.t1 |
| chr4.1 | CDS | 41057460 | 41057542 | 41057460 | ID=cds.MS.gene09138.t1;Parent=MS.gene09138.t1 |
| chr4.1 | exon | 41057238 | 41057364 | 41057238 | ID=MS.gene09138.t1.exon2;Parent=MS.gene09138.t1 |
| chr4.1 | CDS | 41057238 | 41057364 | 41057238 | ID=cds.MS.gene09138.t1;Parent=MS.gene09138.t1 |
| Gene Sequence |
| Protein sequence |
