AlfalfaGEDB | MS.gene20110

M. sativa cultivar XinJiangDaYe / MS.gene20110

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20110.t1	RHN80254.1	89.2	250	24	2	1	249	335	582	2.00E-110	408.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20110.t1	Q84JT7	41.4	181	102	2	45	222	352	531	4.8e-33	142.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20110.t1	A0A396JSL4	89.2	250	24	2	1	249	335	582	1.4e-110	408.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050750	MS.gene20110	0.813143	2.97E-51	-1.69E-46
MS.gene050953	MS.gene20110	0.8212	4.58E-53	-1.69E-46
MS.gene052190	MS.gene20110	0.818315	2.09E-52	-1.69E-46
MS.gene052333	MS.gene20110	0.812748	3.63E-51	-1.69E-46
MS.gene052519	MS.gene20110	0.806673	7.34E-50	-1.69E-46
MS.gene052926	MS.gene20110	0.801714	7.90E-49	-1.69E-46
MS.gene053054	MS.gene20110	-0.826431	2.72E-54	-1.69E-46
MS.gene053221	MS.gene20110	0.807519	4.86E-50	-1.69E-46
MS.gene05415	MS.gene20110	0.812122	4.97E-51	-1.69E-46
MS.gene054291	MS.gene20110	-0.814451	1.53E-51	-1.69E-46
MS.gene054405	MS.gene20110	0.8185	1.90E-52	-1.69E-46
MS.gene054559	MS.gene20110	0.826655	2.41E-54	-1.69E-46
MS.gene054657	MS.gene20110	0.800481	1.41E-48	-1.69E-46
MS.gene055356	MS.gene20110	0.812122	4.97E-51	-1.69E-46
MS.gene055357	MS.gene20110	0.806115	9.62E-50	-1.69E-46
MS.gene055818	MS.gene20110	0.820741	5.85E-53	-1.69E-46
MS.gene056173	MS.gene20110	0.806476	8.07E-50	-1.69E-46
MS.gene056366	MS.gene20110	0.819673	1.03E-52	-1.69E-46
MS.gene056506	MS.gene20110	0.807102	5.95E-50	-1.69E-46
MS.gene056792	MS.gene20110	0.832989	6.90E-56	-1.69E-46
MS.gene057021	MS.gene20110	0.814695	1.35E-51	-1.69E-46
MS.gene057060	MS.gene20110	0.806103	9.68E-50	-1.69E-46
MS.gene058829	MS.gene20110	0.830376	3.04E-55	-1.69E-46
MS.gene060572	MS.gene20110	0.824623	7.30E-54	-1.69E-46
MS.gene060634	MS.gene20110	-0.82516	5.45E-54	-1.69E-46
MS.gene061114	MS.gene20110	-0.804224	2.39E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20110.t1	MTR_1g073380	92.400	250	18	1	1	249	343	592	5.71e-166	471
MS.gene20110.t1	MTR_1g073530	88.800	250	25	2	1	249	335	582	1.22e-152	437
MS.gene20110.t1	MTR_1g073590	88.400	250	26	2	1	249	332	579	1.00e-151	435
MS.gene20110.t1	MTR_8g098645	74.104	251	63	2	1	249	90	340	8.14e-132	376
MS.gene20110.t1	MTR_8g098605	70.000	250	50	2	1	249	365	590	5.08e-115	342
MS.gene20110.t1	MTR_8g098635	80.208	192	37	1	58	248	99	290	1.74e-108	315
MS.gene20110.t1	MTR_8g098635	80.208	192	37	1	58	248	182	373	4.65e-107	314
MS.gene20110.t1	MTR_0259s0060	63.536	181	18	3	51	230	14	147	3.06e-69	211
MS.gene20110.t1	MTR_7g078120	48.404	188	96	1	53	239	387	574	5.54e-56	189
MS.gene20110.t1	MTR_7g078120	48.404	188	96	1	53	239	387	574	1.25e-55	189
MS.gene20110.t1	MTR_8g028905	45.161	186	98	3	53	234	408	593	1.21e-47	166
MS.gene20110.t1	MTR_6g071350	72.549	102	28	0	54	155	273	374	5.34e-45	155
MS.gene20110.t1	MTR_1g073420	91.429	70	5	1	181	249	59	128	2.58e-39	133
MS.gene20110.t1	MTR_5g048010	60.000	100	25	1	56	155	205	289	4.23e-29	112
MS.gene20110.t1	MTR_7g066890	37.059	170	99	3	53	216	194	361	1.19e-28	111
MS.gene20110.t1	MTR_7g066890	37.059	170	99	3	53	216	296	463	5.85e-28	111
MS.gene20110.t1	MTR_3g023780	37.500	200	108	5	35	221	131	326	8.64e-28	111
MS.gene20110.t1	MTR_6g071355	56.701	97	22	2	144	239	1	78	1.94e-24	93.2
MS.gene20110.t1	MTR_7g066880	30.588	170	110	3	53	216	186	353	1.51e-18	84.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20110.t1	AT4G11400	46.798	203	98	5	51	249	372	568	4.78e-53	180
MS.gene20110.t1	AT4G11400	46.798	203	98	5	51	249	372	568	4.78e-53	180
MS.gene20110.t1	AT2G46040	40.884	181	103	2	45	222	352	531	6.94e-36	134
MS.gene20110.t1	AT2G46040	40.884	181	103	2	45	222	352	531	6.94e-36	134
MS.gene20110.t1	AT2G46040	40.884	181	103	2	45	222	366	545	9.09e-36	134
MS.gene20110.t1	AT5G04110	36.646	161	90	4	53	202	361	520	5.97e-28	112
MS.gene20110.t1	AT5G04110	36.646	161	90	4	53	202	361	520	5.97e-28	112
MS.gene20110.t1	AT5G04110	36.646	161	90	4	53	202	362	521	6.18e-28	111
MS.gene20110.t1	AT1G26580	31.980	197	114	4	51	229	67	261	6.53e-24	99.8
MS.gene20110.t1	AT1G26580	31.980	197	114	4	51	229	133	327	9.53e-24	99.8
MS.gene20110.t1	AT1G26580	31.980	197	114	4	51	229	133	327	9.53e-24	99.8
MS.gene20110.t1	AT1G13880	36.413	184	97	6	51	221	127	303	1.90e-22	95.5
MS.gene20110.t1	AT1G13880	36.413	184	97	6	51	221	126	302	1.94e-22	95.5
MS.gene20110.t1	AT1G13880	36.413	184	97	6	51	221	136	312	2.02e-22	95.5
MS.gene20110.t1	AT2G03470	38.732	142	83	3	91	228	164	305	4.49e-21	91.7
MS.gene20110.t1	AT2G03470	38.732	142	83	3	91	228	163	304	4.64e-21	91.7
MS.gene20110.t1	AT5G04110	36.986	146	79	5	53	186	361	505	2.27e-20	90.1

Find 38 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCATTGGGAATTTGATAAAA+TGG	0.169328	7.2:-57260066	MS.gene20110:CDS
CAATGGACTGGTGTAGTTTA+TGG	0.202655	7.2:-57260284	MS.gene20110:CDS
TCACGAGAATCATCACTAAA+TGG	0.231296	7.2:+57260350	None:intergenic
GATGATGACGAAGTCGAATT+TGG	0.269756	7.2:-57259825	MS.gene20110:CDS
TGATGAAGAAAAGACATTTA+AGG	0.313271	7.2:-57260015	MS.gene20110:CDS
GATGAAGCTGAAGTTTGAAT+TGG	0.326776	7.2:-57260102	MS.gene20110:CDS
GGTCTTGAATCATCTTTAAC+AGG	0.340484	7.2:+57260227	None:intergenic
TTCTTCTTGATCACAACATC+TGG	0.358660	7.2:+57260455	None:intergenic
AATCGTTAGGCACACAAGTA+TGG	0.361974	7.2:-57260250	MS.gene20110:CDS
CATTGGGAATTTGATAAAAT+GGG	0.369620	7.2:-57260065	MS.gene20110:CDS
GAAGTCGAATTTGGATCTTT+TGG	0.370187	7.2:-57259816	MS.gene20110:CDS
CACTCCTTGAATTCAGTTGA+TGG	0.388773	7.2:+57259765	None:intergenic
CGTGATAAGTTTGTTTCTGT+TGG	0.390129	7.2:-57260332	MS.gene20110:CDS
AATGGACTGGTGTAGTTTAT+GGG	0.405764	7.2:-57260283	MS.gene20110:CDS
AAACTACACCAGTCCATTGC+GGG	0.425596	7.2:+57260288	None:intergenic
GTGTCCATCAACTGAATTCA+AGG	0.433131	7.2:-57259769	MS.gene20110:CDS
TTTCTTCATCACCAGTCCAT+TGG	0.435383	7.2:+57260027	None:intergenic
ATGAAGCTGAAGTTTGAATT+GGG	0.447468	7.2:-57260101	MS.gene20110:CDS
CGTCCAGTTACTTTCAAAAG+AGG	0.455230	7.2:-57259941	MS.gene20110:CDS
GATTGCATATTGCTGCAAAC+AGG	0.460811	7.2:-57260124	MS.gene20110:CDS
GCTACTGAAACAGATCTTGT+TGG	0.488100	7.2:-57260203	MS.gene20110:CDS
CACGAGAATCATCACTAAAT+GGG	0.488835	7.2:+57260351	None:intergenic
TCCATTGCGGGACTTCCACT+TGG	0.490081	7.2:+57260300	None:intergenic
AGTCCCATGGAGAAAACAAC+TGG	0.495312	7.2:-57260482	None:intergenic
GGGACTTCCACTTGGAAAAG+AGG	0.495364	7.2:+57260308	None:intergenic
ACAAGATCTGTTTCAGTAGC+AGG	0.496101	7.2:+57260206	None:intergenic
GTGGAAGTCCCGCAATGGAC+TGG	0.500938	7.2:-57260296	MS.gene20110:CDS
GGGAGTGACTCTAAATCGTT+AGG	0.521416	7.2:-57260263	MS.gene20110:CDS
CTATCAGAATCGAGTGACTT+CGG	0.542718	7.2:-57259862	MS.gene20110:CDS
GCGAGGAAGTTTCACTCCAA+TGG	0.542815	7.2:-57260043	MS.gene20110:CDS
GGAATTTGATAAAATGGGCG+AGG	0.560905	7.2:-57260060	MS.gene20110:CDS
TCCAAGTGGAAGTCCCGCAA+TGG	0.584534	7.2:-57260301	MS.gene20110:CDS
GAAGTTTCACTCCAATGGAC+TGG	0.584625	7.2:-57260038	MS.gene20110:CDS
GAAACAGATCTTGTTGGTAG+AGG	0.588593	7.2:-57260197	MS.gene20110:CDS
AGTGTTCAGAGAACAAGTAA+TGG	0.612428	7.2:-57259746	None:intergenic
CTTGTTGGTAGAGGAAGACG+AGG	0.623018	7.2:-57260188	MS.gene20110:CDS
TAAACTACACCAGTCCATTG+CGG	0.655876	7.2:+57260287	None:intergenic
AAAGAGGACAAGAAAAGACA+TGG	0.658301	7.2:-57259925	MS.gene20110:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CATTGGGAATTTGATAAAAT+GGG	-	chr7.2:57260160-57260179	MS.gene20110:CDS	25.0%
!	TGATGAAGAAAAGACATTTA+AGG	-	chr7.2:57260210-57260229	MS.gene20110:CDS	25.0%
!!	AGTTCCAAAAATACTTGTTT+TGG	+	chr7.2:57260261-57260280	None:intergenic	25.0%
!!!	GTTCCAAAAATACTTGTTTT+GGG	+	chr7.2:57260260-57260279	None:intergenic	25.0%
	ATGAAGCTGAAGTTTGAATT+GGG	-	chr7.2:57260124-57260143	MS.gene20110:CDS	30.0%
	CCATTGGGAATTTGATAAAA+TGG	-	chr7.2:57260159-57260178	MS.gene20110:CDS	30.0%
!	CCATTTTATCAAATTCCCAA+TGG	+	chr7.2:57260162-57260181	None:intergenic	30.0%
!!!	AAAAATACTTGTTTTGGGAG+GGG	+	chr7.2:57260255-57260274	None:intergenic	30.0%
!!!	CAAAAATACTTGTTTTGGGA+GGG	+	chr7.2:57260256-57260275	None:intergenic	30.0%
	AAAGAGGACAAGAAAAGACA+TGG	-	chr7.2:57260300-57260319	MS.gene20110:CDS	35.0%
	CACGAGAATCATCACTAAAT+GGG	+	chr7.2:57259877-57259896	None:intergenic	35.0%
	GATGAAGCTGAAGTTTGAAT+TGG	-	chr7.2:57260123-57260142	MS.gene20110:CDS	35.0%
	GGTCTTGAATCATCTTTAAC+AGG	+	chr7.2:57260001-57260020	None:intergenic	35.0%
	TCACGAGAATCATCACTAAA+TGG	+	chr7.2:57259878-57259897	None:intergenic	35.0%
	TTCTTCTTGATCACAACATC+TGG	+	chr7.2:57259773-57259792	None:intergenic	35.0%
!	CCTCCCAAAACAAGTATTTT+TGG	-	chr7.2:57260254-57260273	MS.gene20110:CDS	35.0%
!	GAAGTCGAATTTGGATCTTT+TGG	-	chr7.2:57260409-57260428	MS.gene20110:CDS	35.0%
!!	AATGGACTGGTGTAGTTTAT+GGG	-	chr7.2:57259942-57259961	MS.gene20110:CDS	35.0%
!!	CGTGATAAGTTTGTTTCTGT+TGG	-	chr7.2:57259893-57259912	MS.gene20110:CDS	35.0%
!!	CTCTTTTGAAAGTAACTGGA+CGG	+	chr7.2:57260286-57260305	None:intergenic	35.0%
!!	TGTCCTCTTTTGAAAGTAAC+TGG	+	chr7.2:57260290-57260309	None:intergenic	35.0%
!!	TTTGGATCTTTTGGTGGTTT+CGG	-	chr7.2:57260418-57260437	MS.gene20110:CDS	35.0%
!!!	CCAAAAATACTTGTTTTGGG+AGG	+	chr7.2:57260257-57260276	None:intergenic	35.0%
	AATCGTTAGGCACACAAGTA+TGG	-	chr7.2:57259975-57259994	MS.gene20110:CDS	40.0%
	ACAAGATCTGTTTCAGTAGC+AGG	+	chr7.2:57260022-57260041	None:intergenic	40.0%
	CACTCCTTGAATTCAGTTGA+TGG	+	chr7.2:57260463-57260482	None:intergenic	40.0%
	CTATCAGAATCGAGTGACTT+CGG	-	chr7.2:57260363-57260382	MS.gene20110:CDS	40.0%
	GATTGCATATTGCTGCAAAC+AGG	-	chr7.2:57260101-57260120	MS.gene20110:CDS	40.0%
	GCTACTGAAACAGATCTTGT+TGG	-	chr7.2:57260022-57260041	MS.gene20110:CDS	40.0%
	GGAATTTGATAAAATGGGCG+AGG	-	chr7.2:57260165-57260184	MS.gene20110:CDS	40.0%
	GTGTCCATCAACTGAATTCA+AGG	-	chr7.2:57260456-57260475	MS.gene20110:CDS	40.0%
	TAAACTACACCAGTCCATTG+CGG	+	chr7.2:57259941-57259960	None:intergenic	40.0%
	TTTCTTCATCACCAGTCCAT+TGG	+	chr7.2:57260201-57260220	None:intergenic	40.0%
!	CGTCCAGTTACTTTCAAAAG+AGG	-	chr7.2:57260284-57260303	MS.gene20110:CDS	40.0%
!	GAAACAGATCTTGTTGGTAG+AGG	-	chr7.2:57260028-57260047	MS.gene20110:CDS	40.0%
!	GATGATGACGAAGTCGAATT+TGG	-	chr7.2:57260400-57260419	MS.gene20110:CDS	40.0%
!	GGGTTCTGCATTTTACCATT+GGG	-	chr7.2:57260144-57260163	MS.gene20110:CDS	40.0%
!	GTCGAATTTGGATCTTTTGG+TGG	-	chr7.2:57260412-57260431	MS.gene20110:CDS	40.0%
!	TGGGTTCTGCATTTTACCAT+TGG	-	chr7.2:57260143-57260162	MS.gene20110:CDS	40.0%
!!	CAATGGACTGGTGTAGTTTA+TGG	-	chr7.2:57259941-57259960	MS.gene20110:CDS	40.0%
!!	TTGGATCTTTTGGTGGTTTC+GGG	-	chr7.2:57260419-57260438	MS.gene20110:CDS	40.0%
!!!	CTTTTTTCTCACAGCTGCTT+TGG	+	chr7.2:57259807-57259826	None:intergenic	40.0%
	AAACTACACCAGTCCATTGC+GGG	+	chr7.2:57259940-57259959	None:intergenic	45.0%
	GAAGTTTCACTCCAATGGAC+TGG	-	chr7.2:57260187-57260206	MS.gene20110:CDS	45.0%
	GGGAGTGACTCTAAATCGTT+AGG	-	chr7.2:57259962-57259981	MS.gene20110:CDS	45.0%
!	TGTTGGTCCTCTTTTCCAAG+TGG	-	chr7.2:57259910-57259929	MS.gene20110:CDS	45.0%
	GCGAGGAAGTTTCACTCCAA+TGG	-	chr7.2:57260182-57260201	MS.gene20110:CDS	50.0%
	GGGACTTCCACTTGGAAAAG+AGG	+	chr7.2:57259920-57259939	None:intergenic	50.0%
!	CTTGTTGGTAGAGGAAGACG+AGG	-	chr7.2:57260037-57260056	MS.gene20110:CDS	50.0%
!!	GATCTTTTGGTGGTTTCGGG+AGG	-	chr7.2:57260422-57260441	MS.gene20110:CDS	50.0%
	TCCAAGTGGAAGTCCCGCAA+TGG	-	chr7.2:57259924-57259943	MS.gene20110:CDS	55.0%
	TCCATTGCGGGACTTCCACT+TGG	+	chr7.2:57259928-57259947	None:intergenic	55.0%
	GTGGAAGTCCCGCAATGGAC+TGG	-	chr7.2:57259929-57259948	MS.gene20110:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	57259749	57260498	57259749	ID=MS.gene20110
chr7.2	mRNA	57259749	57260498	57259749	ID=MS.gene20110.t1;Parent=MS.gene20110
chr7.2	exon	57259749	57260498	57259749	ID=MS.gene20110.t1.exon1;Parent=MS.gene20110.t1
chr7.2	CDS	57259749	57260498	57259749	ID=cds.MS.gene20110.t1;Parent=MS.gene20110.t1

Gene Sequence

>MS.gene20110

ATGGAGAAAACAACTGGCAAACCAGATGTTGTGATCAAGAAGAAAAAATCAATTGCCAAAGCAGCTGTGAGAAAAAAGAAAAAATCAACTGCAAATGCAGCTGTGACTGAAGAGAAAAAATATGACCCATTTAGTGATGATTCTCGTGATAAGTTTGTTTCTGTTGGTCCTCTTTTCCAAGTGGAAGTCCCGCAATGGACTGGTGTAGTTTATGGGAGTGACTCTAAATCGTTAGGCACACAAGTATGGCCTGTTAAAGATGATTCAAGACCTGCTACTGAAACAGATCTTGTTGGTAGAGGAAGACGAGGAAAGTGTAGCTGCAATGTTCAATGTTCAGTTGACTGTGTAAGATTGCATATTGCTGCAAACAGGATGAAGCTGAAGTTTGAATTGGGTTCTGCATTTTACCATTGGGAATTTGATAAAATGGGCGAGGAAGTTTCACTCCAATGGACTGGTGATGAAGAAAAGACATTTAAGGATATAATGAGTTTAAAAATCCCCTCCCAAAACAAGTATTTTTGGAACTATCCGTCCAGTTACTTTCAAAAGAGGACAAGAAAAGACATGGTGAGATATTACTTCAATGTATATCTTATTCAACTAAGAAGCTATCAGAATCGAGTGACTTCGGAGACTGTTGACAGTGATGATGACGAAGTCGAATTTGGATCTTTTGGTGGTTTCGGGAGGAAAGCTATCAAGTGTCCATCAACTGAATTCAAGGAGTGTTCAGAGAACAAGTAA

Protein sequence

>MS.gene20110.t1

MEKTTGKPDVVIKKKKSIAKAAVRKKKKSTANAAVTEEKKYDPFSDDSRDKFVSVGPLFQVEVPQWTGVVYGSDSKSLGTQVWPVKDDSRPATETDLVGRGRRGKCSCNVQCSVDCVRLHIAANRMKLKFELGSAFYHWEFDKMGEEVSLQWTGDEEKTFKDIMSLKIPSQNKYFWNYPSSYFQKRTRKDMVRYYFNVYLIQLRSYQNRVTSETVDSDDDEVEFGSFGGFGRKAIKCPSTEFKECSENK