Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20847.t1 | XP_003591419.1 | 96.4 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 1.20E-102 | 382.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20847.t1 | G7IDR9 | 96.4 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 8.4e-103 | 382.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene20847 | 0.865229 | 6.87E-65 | -1.69E-46 |
| MS.gene049254 | MS.gene20847 | 0.801097 | 1.06E-48 | -1.69E-46 |
| MS.gene049350 | MS.gene20847 | 0.802907 | 4.49E-49 | -1.69E-46 |
| MS.gene049807 | MS.gene20847 | 0.801012 | 1.10E-48 | -1.69E-46 |
| MS.gene049887 | MS.gene20847 | 0.802726 | 4.89E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene20847 | 0.82377 | 1.16E-53 | -1.69E-46 |
| MS.gene051388 | MS.gene20847 | 0.807882 | 4.07E-50 | -1.69E-46 |
| MS.gene051490 | MS.gene20847 | 0.809501 | 1.83E-50 | -1.69E-46 |
| MS.gene051700 | MS.gene20847 | 0.817324 | 3.50E-52 | -1.69E-46 |
| MS.gene052463 | MS.gene20847 | 0.804982 | 1.66E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene20847 | 0.803225 | 3.86E-49 | -1.69E-46 |
| MS.gene053164 | MS.gene20847 | 0.814675 | 1.37E-51 | -1.69E-46 |
| MS.gene05458 | MS.gene20847 | 0.80824 | 3.41E-50 | -1.69E-46 |
| MS.gene054775 | MS.gene20847 | 0.802145 | 6.45E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene20847 | 0.823186 | 1.59E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene20847 | 0.821573 | 3.76E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene20847 | 0.828749 | 7.56E-55 | -1.69E-46 |
| MS.gene055469 | MS.gene20847 | 0.805881 | 1.08E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene20847 | 0.80841 | 3.14E-50 | -1.69E-46 |
| MS.gene055608 | MS.gene20847 | 0.82681 | 2.21E-54 | -1.69E-46 |
| MS.gene056308 | MS.gene20847 | 0.808257 | 3.38E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene20847 | 0.806844 | 6.75E-50 | -1.69E-46 |
| MS.gene057102 | MS.gene20847 | 0.800562 | 1.36E-48 | -1.69E-46 |
| MS.gene05897 | MS.gene20847 | 0.811679 | 6.21E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene20847 | 0.811999 | 5.28E-51 | -1.69E-46 |
| MS.gene061100 | MS.gene20847 | 0.818234 | 2.18E-52 | -1.69E-46 |
| MS.gene061188 | MS.gene20847 | 0.801834 | 7.47E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20847.t1 | MTR_1g087230 | 96.354 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 9.45e-139 | 385 |
| MS.gene20847.t1 | MTR_7g109410 | 56.345 | 197 | 76 | 5 | 1 | 192 | 1 | 192 | 4.62e-72 | 216 |
| MS.gene20847.t1 | MTR_8g107420 | 27.841 | 176 | 112 | 4 | 1 | 176 | 1 | 161 | 4.90e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20847.t1 | AT4G02090 | 37.273 | 220 | 88 | 10 | 1 | 189 | 1 | 201 | 6.31e-32 | 114 |
Find 42 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTTGAATAGGCAGATTTC+AGG | 0.248523 | 1.3:+61709124 | MS.gene20847:CDS |
| GCCTAGCTTGGATACTATTA+AGG | 0.311308 | 1.3:+61709159 | MS.gene20847:CDS |
| GGTAAAGTTGTATTCTTAGA+TGG | 0.327818 | 1.3:+61708695 | MS.gene20847:CDS |
| GTTCTTATAAGTTTCTTCCT+TGG | 0.362756 | 1.3:+61708975 | MS.gene20847:CDS |
| ATCTGCCTATTCAAATAATC+TGG | 0.371640 | 1.3:-61709118 | None:intergenic |
| GGAAGAGGTGATGGAGGAGA+AGG | 0.379095 | 1.3:+61709045 | MS.gene20847:CDS |
| TCTTCGCCGCTTAAACCAAT+AGG | 0.383885 | 1.3:-61708896 | None:intergenic |
| ATAGGCTTCCCTTGCTTCAC+TGG | 0.390684 | 1.3:-61708878 | None:intergenic |
| GGAGAATTTGCCGGACATAT+TGG | 0.397303 | 1.3:+61709087 | MS.gene20847:CDS |
| GTGAAGCAAGGGAAGCCTAT+TGG | 0.415059 | 1.3:+61708881 | MS.gene20847:CDS |
| TTTCGTCCTTCAGATTCAAC+AGG | 0.424376 | 1.3:+61708674 | MS.gene20847:CDS |
| ATCTCTAGCTTCTTGTCAGC+AGG | 0.426386 | 1.3:-61708833 | None:intergenic |
| GGAGGCCAGATTATTTGAAT+AGG | 0.439315 | 1.3:+61709113 | MS.gene20847:CDS |
| AGCTTCTTGTCAGCAGGTAA+TGG | 0.466025 | 1.3:-61708827 | None:intergenic |
| ATGTCATAACAGTGAAATAG+TGG | 0.471468 | 1.3:+61709009 | MS.gene20847:CDS |
| GAAGAGGTGATGGAGGAGAA+GGG | 0.490391 | 1.3:+61709046 | MS.gene20847:CDS |
| GAAAGGGTGGAAGCCTAGCT+TGG | 0.506451 | 1.3:+61709147 | MS.gene20847:CDS |
| TCGGAGGAAGGAAGAGGTGA+TGG | 0.513945 | 1.3:+61709036 | MS.gene20847:CDS |
| TTGTCAGCAGGTAATGGTGT+TGG | 0.520247 | 1.3:-61708821 | None:intergenic |
| ATTTGAATAGGCAGATTTCA+GGG | 0.521145 | 1.3:+61709125 | MS.gene20847:CDS |
| AATAGGCAGATTTCAGGGAA+AGG | 0.530769 | 1.3:+61709130 | MS.gene20847:CDS |
| GGCCTCCCTTCCAATATGTC+CGG | 0.533242 | 1.3:-61709097 | None:intergenic |
| TGATGCTTCCAGTGAAGCAA+GGG | 0.535617 | 1.3:+61708870 | MS.gene20847:CDS |
| AACAGTGGCAGAGTTAATGA+TGG | 0.548929 | 1.3:+61708745 | MS.gene20847:CDS |
| ATAGGCAGATTTCAGGGAAA+GGG | 0.549284 | 1.3:+61709131 | MS.gene20847:CDS |
| GGAGCCTCGGAGGAAGGAAG+AGG | 0.553960 | 1.3:+61709030 | MS.gene20847:CDS |
| TTGATGCTTCCAGTGAAGCA+AGG | 0.559013 | 1.3:+61708869 | MS.gene20847:CDS |
| ACTTTACCTGTTGAATCTGA+AGG | 0.560942 | 1.3:-61708680 | None:intergenic |
| AATTTGCCGGACATATTGGA+AGG | 0.563693 | 1.3:+61709091 | MS.gene20847:CDS |
| TCCTTAATAGTATCCAAGCT+AGG | 0.569966 | 1.3:-61709160 | None:intergenic |
| AATAGTGGAGCCTCGGAGGA+AGG | 0.571841 | 1.3:+61709024 | MS.gene20847:CDS |
| GGGAAGCCTATTGGTTTAAG+CGG | 0.581568 | 1.3:+61708890 | MS.gene20847:CDS |
| ACAGTGAAATAGTGGAGCCT+CGG | 0.582711 | 1.3:+61709017 | MS.gene20847:CDS |
| GAGGAAGGAAGAGGTGATGG+AGG | 0.589938 | 1.3:+61709039 | MS.gene20847:CDS |
| GGCAGATTTCAGGGAAAGGG+TGG | 0.595138 | 1.3:+61709134 | MS.gene20847:CDS |
| AATTAACCATCAACAAAACT+CGG | 0.602000 | 1.3:-61708925 | None:intergenic |
| TGCCGGACATATTGGAAGGG+AGG | 0.607569 | 1.3:+61709095 | MS.gene20847:CDS |
| GTGAAATAGTGGAGCCTCGG+AGG | 0.619098 | 1.3:+61709020 | MS.gene20847:CDS |
| TGACATAAATTACTAAACCA+AGG | 0.652368 | 1.3:-61708992 | None:intergenic |
| ATTTGCCGGACATATTGGAA+GGG | 0.657578 | 1.3:+61709092 | MS.gene20847:CDS |
| ATCACCTCTTCCTTCCTCCG+AGG | 0.665878 | 1.3:-61709034 | None:intergenic |
| ATTTGATGAAGCAATAACAG+TGG | 0.710910 | 1.3:+61708730 | MS.gene20847:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTAACCATCAACAAAACT+CGG | - | chr1.3:61708928-61708947 | None:intergenic | 25.0% |
| ! | TGACATAAATTACTAAACCA+AGG | - | chr1.3:61708995-61709014 | None:intergenic | 25.0% |
| ! | TTGAGAAAAAACATACTCAT+TGG | + | chr1.3:61709191-61709210 | MS.gene20847:CDS | 25.0% |
| ATCTGCCTATTCAAATAATC+TGG | - | chr1.3:61709121-61709140 | None:intergenic | 30.0% | |
| ATGTCATAACAGTGAAATAG+TGG | + | chr1.3:61709009-61709028 | MS.gene20847:CDS | 30.0% | |
| ATTTGAATAGGCAGATTTCA+GGG | + | chr1.3:61709125-61709144 | MS.gene20847:CDS | 30.0% | |
| ATTTGATGAAGCAATAACAG+TGG | + | chr1.3:61708730-61708749 | MS.gene20847:CDS | 30.0% | |
| GGTAAAGTTGTATTCTTAGA+TGG | + | chr1.3:61708695-61708714 | MS.gene20847:CDS | 30.0% | |
| GTTCTTATAAGTTTCTTCCT+TGG | + | chr1.3:61708975-61708994 | MS.gene20847:CDS | 30.0% | |
| TATTTGAATAGGCAGATTTC+AGG | + | chr1.3:61709124-61709143 | MS.gene20847:CDS | 30.0% | |
| !!! | AACTCAACTACTACTTTTTG+TGG | - | chr1.3:61708776-61708795 | None:intergenic | 30.0% |
| ACTTTACCTGTTGAATCTGA+AGG | - | chr1.3:61708683-61708702 | None:intergenic | 35.0% | |
| ATTCAGCTGTGAACCAAAAA+AGG | + | chr1.3:61708798-61708817 | MS.gene20847:CDS | 35.0% | |
| TCCTTAATAGTATCCAAGCT+AGG | - | chr1.3:61709163-61709182 | None:intergenic | 35.0% | |
| AACAGTGGCAGAGTTAATGA+TGG | + | chr1.3:61708745-61708764 | MS.gene20847:CDS | 40.0% | |
| AATAGGCAGATTTCAGGGAA+AGG | + | chr1.3:61709130-61709149 | MS.gene20847:CDS | 40.0% | |
| AATTTGCCGGACATATTGGA+AGG | + | chr1.3:61709091-61709110 | MS.gene20847:CDS | 40.0% | |
| ATAGGCAGATTTCAGGGAAA+GGG | + | chr1.3:61709131-61709150 | MS.gene20847:CDS | 40.0% | |
| ATTTGCCGGACATATTGGAA+GGG | + | chr1.3:61709092-61709111 | MS.gene20847:CDS | 40.0% | |
| GCCTAGCTTGGATACTATTA+AGG | + | chr1.3:61709159-61709178 | MS.gene20847:CDS | 40.0% | |
| GGAGGCCAGATTATTTGAAT+AGG | + | chr1.3:61709113-61709132 | MS.gene20847:CDS | 40.0% | |
| TTTCGTCCTTCAGATTCAAC+AGG | + | chr1.3:61708674-61708693 | MS.gene20847:CDS | 40.0% | |
| !! | CGATTTTTCGGAGAATTTGC+CGG | + | chr1.3:61709078-61709097 | MS.gene20847:CDS | 40.0% |
| !!! | GTAATGGTGTTGGCCTTTTT+TGG | - | chr1.3:61708814-61708833 | None:intergenic | 40.0% |
| AGCTTCTTGTCAGCAGGTAA+TGG | - | chr1.3:61708830-61708849 | None:intergenic | 45.0% | |
| GGAGAATTTGCCGGACATAT+TGG | + | chr1.3:61709087-61709106 | MS.gene20847:CDS | 45.0% | |
| TCTTCGCCGCTTAAACCAAT+AGG | - | chr1.3:61708899-61708918 | None:intergenic | 45.0% | |
| TGATGCTTCCAGTGAAGCAA+GGG | + | chr1.3:61708870-61708889 | MS.gene20847:CDS | 45.0% | |
| TTGATGCTTCCAGTGAAGCA+AGG | + | chr1.3:61708869-61708888 | MS.gene20847:CDS | 45.0% | |
| ! | ACAGTGAAATAGTGGAGCCT+CGG | + | chr1.3:61709017-61709036 | MS.gene20847:CDS | 45.0% |
| ! | ATCTCTAGCTTCTTGTCAGC+AGG | - | chr1.3:61708836-61708855 | None:intergenic | 45.0% |
| ! | GGGAGAGAGACACGATTTTT+CGG | + | chr1.3:61709066-61709085 | MS.gene20847:CDS | 45.0% |
| ! | TTGTCAGCAGGTAATGGTGT+TGG | - | chr1.3:61708824-61708843 | None:intergenic | 45.0% |
| !! | GGGAAGCCTATTGGTTTAAG+CGG | + | chr1.3:61708890-61708909 | MS.gene20847:CDS | 45.0% |
| !!! | AACTCGCCGAGTTTTGTTGA+TGG | + | chr1.3:61708919-61708938 | MS.gene20847:CDS | 45.0% |
| ATAGGCTTCCCTTGCTTCAC+TGG | - | chr1.3:61708881-61708900 | None:intergenic | 50.0% | |
| GTGAAGCAAGGGAAGCCTAT+TGG | + | chr1.3:61708881-61708900 | MS.gene20847:CDS | 50.0% | |
| !! | GAAGAGGTGATGGAGGAGAA+GGG | + | chr1.3:61709046-61709065 | MS.gene20847:CDS | 50.0% |
| ATCACCTCTTCCTTCCTCCG+AGG | - | chr1.3:61709037-61709056 | None:intergenic | 55.0% | |
| GAAAGGGTGGAAGCCTAGCT+TGG | + | chr1.3:61709147-61709166 | MS.gene20847:CDS | 55.0% | |
| GGCAGATTTCAGGGAAAGGG+TGG | + | chr1.3:61709134-61709153 | MS.gene20847:CDS | 55.0% | |
| GGCCTCCCTTCCAATATGTC+CGG | - | chr1.3:61709100-61709119 | None:intergenic | 55.0% | |
| TCGGAGGAAGGAAGAGGTGA+TGG | + | chr1.3:61709036-61709055 | MS.gene20847:CDS | 55.0% | |
| TGCCGGACATATTGGAAGGG+AGG | + | chr1.3:61709095-61709114 | MS.gene20847:CDS | 55.0% | |
| ! | AATAGTGGAGCCTCGGAGGA+AGG | + | chr1.3:61709024-61709043 | MS.gene20847:CDS | 55.0% |
| ! | GAGGAAGGAAGAGGTGATGG+AGG | + | chr1.3:61709039-61709058 | MS.gene20847:CDS | 55.0% |
| ! | GTGAAATAGTGGAGCCTCGG+AGG | + | chr1.3:61709020-61709039 | MS.gene20847:CDS | 55.0% |
| !! | GGAAGAGGTGATGGAGGAGA+AGG | + | chr1.3:61709045-61709064 | MS.gene20847:CDS | 55.0% |
| GGAGCCTCGGAGGAAGGAAG+AGG | + | chr1.3:61709030-61709049 | MS.gene20847:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 61708656 | 61709234 | 61708656 | ID=MS.gene20847 |
| chr1.3 | mRNA | 61708656 | 61709234 | 61708656 | ID=MS.gene20847.t1;Parent=MS.gene20847 |
| chr1.3 | exon | 61708656 | 61709234 | 61708656 | ID=MS.gene20847.t1.exon1;Parent=MS.gene20847.t1 |
| chr1.3 | CDS | 61708656 | 61709234 | 61708656 | ID=cds.MS.gene20847.t1;Parent=MS.gene20847.t1 |
| Gene Sequence |
| Protein sequence |
