Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22322.t1 | XP_003611015.2 | 94.7 | 1040 | 49 | 3 | 1 | 1040 | 1 | 1034 | 0.00E+00 | 1893.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22322.t1 | Q9T014 | 46.2 | 1078 | 473 | 17 | 2 | 1040 | 27 | 1036 | 4.7e-247 | 855.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22322.t1 | G7KAM7 | 94.7 | 1040 | 49 | 3 | 1 | 1040 | 1 | 1034 | 0.0e+00 | 1893.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049801 | MS.gene22322 | 0.819614 | 1.06E-52 | -1.69E-46 |
| MS.gene050361 | MS.gene22322 | 0.854152 | 1.49E-61 | -1.69E-46 |
| MS.gene050762 | MS.gene22322 | 0.809739 | 1.63E-50 | -1.69E-46 |
| MS.gene050763 | MS.gene22322 | 0.806875 | 6.65E-50 | -1.69E-46 |
| MS.gene051209 | MS.gene22322 | 0.801115 | 1.05E-48 | -1.69E-46 |
| MS.gene051631 | MS.gene22322 | -0.802562 | 5.29E-49 | -1.69E-46 |
| MS.gene051800 | MS.gene22322 | 0.807473 | 4.97E-50 | -1.69E-46 |
| MS.gene052216 | MS.gene22322 | 0.803046 | 4.20E-49 | -1.69E-46 |
| MS.gene052674 | MS.gene22322 | 0.833051 | 6.66E-56 | -1.69E-46 |
| MS.gene052675 | MS.gene22322 | 0.836404 | 9.55E-57 | -1.69E-46 |
| MS.gene052677 | MS.gene22322 | 0.841471 | 4.66E-58 | -1.69E-46 |
| MS.gene052776 | MS.gene22322 | 0.81538 | 9.52E-52 | -1.69E-46 |
| MS.gene052994 | MS.gene22322 | 0.833029 | 6.74E-56 | -1.69E-46 |
| MS.gene053486 | MS.gene22322 | 0.830203 | 3.35E-55 | -1.69E-46 |
| MS.gene054379 | MS.gene22322 | 0.815656 | 8.27E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene22322 | 0.805989 | 1.02E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene22322 | 0.8059 | 1.07E-49 | -1.69E-46 |
| MS.gene056480 | MS.gene22322 | 0.837978 | 3.78E-57 | -1.69E-46 |
| MS.gene05761 | MS.gene22322 | 0.82136 | 4.21E-53 | -1.69E-46 |
| MS.gene057869 | MS.gene22322 | 0.80903 | 2.31E-50 | -1.69E-46 |
| MS.gene057929 | MS.gene22322 | 0.823989 | 1.03E-53 | -1.69E-46 |
| MS.gene058081 | MS.gene22322 | 0.824244 | 8.97E-54 | -1.69E-46 |
| MS.gene060296 | MS.gene22322 | 0.82082 | 5.61E-53 | -1.69E-46 |
| MS.gene06052 | MS.gene22322 | 0.831563 | 1.56E-55 | -1.69E-46 |
| MS.gene060683 | MS.gene22322 | 0.800362 | 1.49E-48 | -1.69E-46 |
| MS.gene060802 | MS.gene22322 | 0.812851 | 3.44E-51 | -1.69E-46 |
| MS.gene060850 | MS.gene22322 | 0.816574 | 5.16E-52 | -1.69E-46 |
| MS.gene060851 | MS.gene22322 | 0.808852 | 2.53E-50 | -1.69E-46 |
| MS.gene060852 | MS.gene22322 | 0.806958 | 6.39E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene22322 | 0.800566 | 1.36E-48 | -1.69E-46 |
| MS.gene06121 | MS.gene22322 | 0.8061 | 9.69E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene49369 | MS.gene22322 | PPI |
| MS.gene22322 | MS.gene50554 | PPI |
| MS.gene22322 | MS.gene036180 | PPI |
| MS.gene39936 | MS.gene22322 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22322.t1 | MTR_5g009530 | 95.385 | 1040 | 42 | 3 | 1 | 1040 | 1 | 1034 | 0.0 | 2044 |
| MS.gene22322.t1 | MTR_5g009530 | 95.097 | 979 | 42 | 3 | 1 | 979 | 1 | 973 | 0.0 | 1917 |
| MS.gene22322.t1 | MTR_5g009530 | 93.905 | 804 | 43 | 3 | 1 | 804 | 1 | 798 | 0.0 | 1552 |
| MS.gene22322.t1 | MTR_8g027985 | 51.250 | 800 | 334 | 15 | 275 | 1040 | 187 | 964 | 0.0 | 728 |
| MS.gene22322.t1 | MTR_2g085210 | 41.162 | 826 | 412 | 15 | 272 | 1040 | 67 | 875 | 7.82e-180 | 546 |
| MS.gene22322.t1 | MTR_5g085250 | 47.399 | 346 | 170 | 3 | 695 | 1039 | 204 | 538 | 2.87e-108 | 348 |
| MS.gene22322.t1 | MTR_5g085250 | 47.399 | 346 | 170 | 3 | 695 | 1039 | 237 | 571 | 2.50e-107 | 346 |
| MS.gene22322.t1 | MTR_5g085250 | 47.399 | 346 | 170 | 3 | 695 | 1039 | 341 | 675 | 9.87e-106 | 345 |
| MS.gene22322.t1 | MTR_2g085210 | 34.609 | 653 | 324 | 14 | 272 | 851 | 67 | 689 | 4.55e-94 | 315 |
| MS.gene22322.t1 | MTR_2g085210 | 34.609 | 653 | 324 | 14 | 272 | 851 | 67 | 689 | 4.66e-94 | 315 |
| MS.gene22322.t1 | MTR_2g016160 | 30.323 | 155 | 105 | 2 | 782 | 933 | 113 | 267 | 5.05e-13 | 71.6 |
| MS.gene22322.t1 | MTR_1g090827 | 23.643 | 258 | 183 | 9 | 789 | 1036 | 21 | 274 | 6.75e-11 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22322.t1 | AT4G11110 | 47.280 | 1066 | 467 | 19 | 8 | 1040 | 33 | 1036 | 0.0 | 883 |
| MS.gene22322.t1 | AT2G46340 | 44.681 | 1081 | 496 | 24 | 2 | 1040 | 9 | 1029 | 0.0 | 800 |
| MS.gene22322.t1 | AT2G46340 | 44.681 | 1081 | 496 | 24 | 2 | 1040 | 9 | 1029 | 0.0 | 800 |
| MS.gene22322.t1 | AT1G53090 | 40.482 | 788 | 391 | 12 | 278 | 1040 | 60 | 794 | 0.0 | 553 |
| MS.gene22322.t1 | AT1G53090 | 40.482 | 788 | 391 | 12 | 278 | 1040 | 60 | 794 | 0.0 | 553 |
| MS.gene22322.t1 | AT1G53090 | 40.482 | 788 | 391 | 12 | 278 | 1040 | 60 | 794 | 0.0 | 553 |
| MS.gene22322.t1 | AT3G15354 | 40.000 | 815 | 407 | 14 | 272 | 1040 | 66 | 844 | 0.0 | 552 |
| MS.gene22322.t1 | AT3G15354 | 39.264 | 815 | 405 | 15 | 272 | 1040 | 66 | 836 | 1.52e-174 | 531 |
| MS.gene22322.t1 | AT3G15354 | 44.649 | 598 | 285 | 9 | 485 | 1040 | 1 | 594 | 7.55e-165 | 498 |
| MS.gene22322.t1 | AT3G15354 | 44.649 | 598 | 285 | 9 | 485 | 1040 | 1 | 594 | 7.55e-165 | 498 |
| MS.gene22322.t1 | AT1G53090 | 46.101 | 577 | 286 | 8 | 485 | 1040 | 1 | 573 | 1.79e-164 | 496 |
| MS.gene22322.t1 | AT2G32950 | 43.659 | 410 | 207 | 5 | 630 | 1039 | 175 | 560 | 6.82e-111 | 355 |
| MS.gene22322.t1 | AT2G32950 | 43.659 | 410 | 207 | 5 | 630 | 1039 | 288 | 673 | 2.93e-109 | 355 |
| MS.gene22322.t1 | AT1G53090 | 34.775 | 601 | 317 | 10 | 278 | 853 | 60 | 610 | 5.38e-99 | 325 |
| MS.gene22322.t1 | AT3G15354 | 34.505 | 626 | 331 | 12 | 272 | 851 | 66 | 658 | 7.06e-99 | 327 |
| MS.gene22322.t1 | AT5G52250 | 34.877 | 324 | 184 | 11 | 727 | 1037 | 71 | 380 | 8.57e-43 | 161 |
| MS.gene22322.t1 | AT5G23730 | 34.627 | 335 | 179 | 12 | 728 | 1036 | 41 | 361 | 1.06e-42 | 160 |
| MS.gene22322.t1 | AT2G21390 | 24.419 | 258 | 181 | 9 | 789 | 1036 | 21 | 274 | 1.76e-11 | 69.3 |
| MS.gene22322.t1 | AT3G49660 | 27.203 | 261 | 164 | 13 | 730 | 981 | 27 | 270 | 4.57e-11 | 65.9 |
Find 216 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAATTCAATTAGTGATAT+TGG | 0.180525 | 5.4:+4152050 | MS.gene22322:CDS |
| TGGAAAGTTTAAATATATTT+AGG | 0.190587 | 5.4:+4152657 | MS.gene22322:CDS |
| AACATCTTCCTCTGTTGATA+TGG | 0.226062 | 5.4:+4152835 | MS.gene22322:CDS |
| CTACAATCTGAAGTGATTAA+TGG | 0.230123 | 5.4:+4155068 | MS.gene22322:CDS |
| TATCAGATTGGAAATAATTC+AGG | 0.230148 | 5.4:+4152023 | MS.gene22322:CDS |
| GTTCTTAGTATATCTTTATC+TGG | 0.244121 | 5.4:-4155497 | None:intergenic |
| TGTTGGATCCACCTCGTTTC+CGG | 0.261043 | 5.4:+4152118 | MS.gene22322:CDS |
| ATTCCGATTCTGCATCTTCT+TGG | 0.266535 | 5.4:-4155133 | None:intergenic |
| TCCTCTCATTTGCTTGCCTT+TGG | 0.266707 | 5.4:+4158298 | MS.gene22322:CDS |
| GACGAGAGTGCGAGGGTTAC+TGG | 0.269493 | 5.4:+4152878 | MS.gene22322:CDS |
| ATTTAGGAAAATTGTGGATT+TGG | 0.269624 | 5.4:+4152673 | MS.gene22322:CDS |
| CATTCAGTCGTGCTAGATTT+AGG | 0.275646 | 5.4:-4152578 | None:intergenic |
| CCAAACTTGTGTGAAGTAAT+TGG | 0.283179 | 5.4:-4159610 | None:intergenic |
| CCAATTACTTCACACAAGTT+TGG | 0.299695 | 5.4:+4159610 | MS.gene22322:CDS |
| CTGATAATTCATTGAAGATC+TGG | 0.300298 | 5.4:+4158452 | MS.gene22322:CDS |
| AAAGTGTTACAAATGGTTTA+AGG | 0.300773 | 5.4:+4159751 | MS.gene22322:CDS |
| AGAGAGCATATAACATTTGC+AGG | 0.304868 | 5.4:-4155668 | None:intergenic |
| TCTTTCACATATAGAATTAC+TGG | 0.313843 | 5.4:-4153068 | None:intergenic |
| GGAATTCAATTAGTGATATT+GGG | 0.319812 | 5.4:+4152051 | MS.gene22322:CDS |
| ATACTCAGCAAACCCTGATT+TGG | 0.324942 | 5.4:-4152270 | None:intergenic |
| CAATCTCAGTTCATTTGTAT+TGG | 0.326529 | 5.4:-4155390 | None:intergenic |
| CATTAATCACTTCAGATTGT+AGG | 0.328405 | 5.4:-4155067 | None:intergenic |
| AATTGGTCTGTGACCCAAAA+AGG | 0.335217 | 5.4:+4155527 | MS.gene22322:CDS |
| GTTCATTTGTATTGGAGATT+GGG | 0.336479 | 5.4:-4155382 | None:intergenic |
| ATTTAATGTAAGATGGATAA+AGG | 0.342528 | 5.4:-4152733 | None:intergenic |
| TGAAGTGATTAATGGATTAC+AGG | 0.351762 | 5.4:+4155076 | MS.gene22322:CDS |
| AGTTCATTTGTATTGGAGAT+TGG | 0.355893 | 5.4:-4155383 | None:intergenic |
| AATTCTTCAACTGTTTCATA+TGG | 0.361630 | 5.4:-4151918 | None:intergenic |
| GCTTTAGGAACCTTCTTTGA+TGG | 0.364422 | 5.4:+4155575 | MS.gene22322:CDS |
| TGTTTCAGAAATCTGAACTT+TGG | 0.364662 | 5.4:-4155459 | None:intergenic |
| CAAAGCTTAGCTGTGTTTGC+TGG | 0.367423 | 5.4:+4155822 | MS.gene22322:CDS |
| GAAGTTCAAATGCCTTCAAT+TGG | 0.370322 | 5.4:+4152935 | MS.gene22322:CDS |
| ACATCTCAACAGCAGGATAA+TGG | 0.370679 | 5.4:-4155790 | None:intergenic |
| ACAAAGATGATATCCAAATC+AGG | 0.375965 | 5.4:+4152257 | MS.gene22322:CDS |
| TACTAAGAACTCGCGAGAAT+TGG | 0.379466 | 5.4:+4155510 | MS.gene22322:CDS |
| TGGCAGAATCTTTATCAGAT+TGG | 0.379730 | 5.4:+4152011 | MS.gene22322:CDS |
| GCGGTGATGCGAATCAGAAT+TGG | 0.379858 | 5.4:+4152393 | MS.gene22322:CDS |
| CATAAAAGTAAAGATGCTTT+AGG | 0.380101 | 5.4:+4155560 | MS.gene22322:CDS |
| CGGTGATGCGAATCAGAATT+GGG | 0.380736 | 5.4:+4152394 | MS.gene22322:CDS |
| CTTCCTTAGGATGTTCTGAT+AGG | 0.384102 | 5.4:-4153812 | None:intergenic |
| CTCTAATTGTTCAACCAACT+TGG | 0.385891 | 5.4:-4155207 | None:intergenic |
| ACATCTTCCTCTGTTGATAT+GGG | 0.391461 | 5.4:+4152836 | MS.gene22322:CDS |
| GAAGATTACTTTGCTTCTGC+TGG | 0.394367 | 5.4:+4155707 | MS.gene22322:CDS |
| AAATCTGGCCAGCGCAAATC+AGG | 0.395977 | 5.4:+4152629 | MS.gene22322:CDS |
| CGCAGCTACGTCTAGTGCTT+GGG | 0.397285 | 5.4:+4152091 | MS.gene22322:CDS |
| CCGCAGCTACGTCTAGTGCT+TGG | 0.400322 | 5.4:+4152090 | MS.gene22322:CDS |
| ATCCCTCGTGTATCTAATTC+AGG | 0.401971 | 5.4:+4153031 | MS.gene22322:CDS |
| CCATCATCACGTCCAACAAC+AGG | 0.401981 | 5.4:+4153867 | MS.gene22322:CDS |
| AGAAAATCCTTGGTTTCCTC+TGG | 0.410497 | 5.4:+4155275 | MS.gene22322:CDS |
| ATCAGTCTCTTTACCAGAGA+TGG | 0.417463 | 5.4:-4159644 | None:intergenic |
| GAAATTCTTTGGGCACAATC+AGG | 0.417682 | 5.4:+4158236 | MS.gene22322:CDS |
| TGATGAAAGAATTCAGTTAG+AGG | 0.419881 | 5.4:+4151782 | MS.gene22322:CDS |
| TTGAGAGAATGGTTAAAATC+TGG | 0.420168 | 5.4:+4152614 | MS.gene22322:CDS |
| TTTCAATAGTACCCTGTTGT+TGG | 0.422547 | 5.4:-4153879 | None:intergenic |
| GCTCGCAGCCTAACCCTGTC+TGG | 0.432747 | 5.4:+4158514 | MS.gene22322:CDS |
| GGATCTATTGATCCCATCTC+TGG | 0.434827 | 5.4:+4159631 | MS.gene22322:CDS |
| AATCTGAACTTTGGATCTCA+TGG | 0.436953 | 5.4:-4155450 | None:intergenic |
| TAGTATGTCCAGACAGGGTT+AGG | 0.437776 | 5.4:-4158522 | None:intergenic |
| GAGTAATGCAGTTGAACATT+TGG | 0.442426 | 5.4:+4152175 | MS.gene22322:CDS |
| CCACTTTGCCTGATTTGCGC+TGG | 0.443062 | 5.4:-4152637 | None:intergenic |
| GGTTGAACAATTAGAGTGCT+TGG | 0.444330 | 5.4:+4155214 | MS.gene22322:CDS |
| GTAATTCTATATGTGAAAGA+TGG | 0.446114 | 5.4:+4153071 | MS.gene22322:CDS |
| GGTGGATGACTCACACTCTC+GGG | 0.449991 | 5.4:+4152694 | MS.gene22322:CDS |
| AAACAAATGAAGACTGGTAT+TGG | 0.453637 | 5.4:+4152476 | MS.gene22322:CDS |
| CAATGTCTGATCTTCATCAT+AGG | 0.456778 | 5.4:+4153772 | MS.gene22322:CDS |
| ATCAGATTGGAAATAATTCA+GGG | 0.462085 | 5.4:+4152024 | MS.gene22322:CDS |
| GTTGGACGTGATGATGGTTC+AGG | 0.462126 | 5.4:-4153861 | None:intergenic |
| GTGGGTTTGTCTGTTGCTGA+TGG | 0.464615 | 5.4:+4159196 | MS.gene22322:CDS |
| AAATATATTTAGGAAAATTG+TGG | 0.466422 | 5.4:+4152667 | MS.gene22322:CDS |
| TGATAATTCATTGAAGATCT+GGG | 0.466607 | 5.4:+4158453 | MS.gene22322:CDS |
| TCAGCTATGGGATGCTAGTA+CGG | 0.467085 | 5.4:+4157084 | MS.gene22322:intron |
| ATTGGAAATAATTCAGGGAA+TGG | 0.468351 | 5.4:+4152029 | MS.gene22322:CDS |
| CCTGTTGTTGGACGTGATGA+TGG | 0.468695 | 5.4:-4153867 | None:intergenic |
| CTCAATAAAACCTCTTCGGT+TGG | 0.468709 | 5.4:+4158478 | MS.gene22322:CDS |
| TATGCTCTCTAAGCTTTGAC+CGG | 0.469053 | 5.4:+4155681 | MS.gene22322:CDS |
| GCATGGCTTAAGAAAATCCT+TGG | 0.470022 | 5.4:+4155265 | MS.gene22322:CDS |
| GTCTGGACATACTAATGAAA+AGG | 0.473081 | 5.4:+4158531 | MS.gene22322:CDS |
| TCATGATTATTAGCAGTCTC+AGG | 0.473314 | 5.4:-4152914 | None:intergenic |
| TTTCATTAGTATGTCCAGAC+AGG | 0.474015 | 5.4:-4158528 | None:intergenic |
| AGTTTGTTTCAAGTGTGTGC+TGG | 0.474338 | 5.4:+4159680 | MS.gene22322:CDS |
| AGTAAGGGTGTTGTACATAA+AGG | 0.475090 | 5.4:+4152314 | MS.gene22322:CDS |
| TTCTTAGTATATCTTTATCT+GGG | 0.477468 | 5.4:-4155496 | None:intergenic |
| AGACAAAATCCAGCTTCCTT+AGG | 0.483629 | 5.4:-4153825 | None:intergenic |
| ATGTATAAAAGTGTTACAAA+TGG | 0.485163 | 5.4:+4159744 | MS.gene22322:CDS |
| TTTGGCGGCTGCGGAAAGTA+AGG | 0.486066 | 5.4:+4152193 | MS.gene22322:CDS |
| AGACCAAGAAGATGCAGAAT+CGG | 0.486392 | 5.4:+4155130 | MS.gene22322:CDS |
| GGTAGCAATTTAATGTAAGA+TGG | 0.487289 | 5.4:-4152740 | None:intergenic |
| AATCCTTGGTTTCCTCTGGC+TGG | 0.487954 | 5.4:+4155279 | MS.gene22322:CDS |
| TTGGAAATAATTCAGGGAAT+GGG | 0.490364 | 5.4:+4152030 | MS.gene22322:CDS |
| AAAACAAACGGCAGGTAAAA+GGG | 0.490416 | 5.4:+4152799 | MS.gene22322:CDS |
| TAGAAGTCCCTGGTGTGTAT+TGG | 0.493208 | 5.4:+4158363 | MS.gene22322:CDS |
| GGATGTTCTGATAGGAAAGC+TGG | 0.493573 | 5.4:-4153804 | None:intergenic |
| CAAACCAGGTCATGTACATT+GGG | 0.496767 | 5.4:+4152765 | MS.gene22322:CDS |
| GGATCTCAATAAAACCTCTT+CGG | 0.497863 | 5.4:+4158474 | MS.gene22322:CDS |
| AGTTGAACATTTGGCGGCTG+CGG | 0.498441 | 5.4:+4152184 | MS.gene22322:CDS |
| TCAGAACATCCTAAGGAAGC+TGG | 0.499024 | 5.4:+4153816 | MS.gene22322:CDS |
| TGGTGGATGACTCACACTCT+CGG | 0.500502 | 5.4:+4152693 | MS.gene22322:CDS |
| GTGAACATGAAAAGAGGGCT+TGG | 0.501727 | 5.4:+4157128 | MS.gene22322:CDS |
| GAGCAAAATCAGACAAAGGC+AGG | 0.503141 | 5.4:+4152371 | MS.gene22322:CDS |
| CTTCTTGCTGCCAATTCAAG+TGG | 0.504977 | 5.4:+4159721 | MS.gene22322:CDS |
| GATATTGGGTTGATTAGTAG+TGG | 0.505458 | 5.4:+4152065 | MS.gene22322:CDS |
| TTCATTTGTATTGGAGATTG+GGG | 0.507234 | 5.4:-4155381 | None:intergenic |
| AAGATTACTTTGCTTCTGCT+GGG | 0.507710 | 5.4:+4155708 | MS.gene22322:CDS |
| TAGGAAAATTGTGGATTTGG+TGG | 0.511869 | 5.4:+4152676 | MS.gene22322:CDS |
| ACTGCAGCATCGCTGTGAAT+CGG | 0.512278 | 5.4:-4152554 | None:intergenic |
| ATGTACATGACCTGGTTTGA+TGG | 0.512767 | 5.4:-4152761 | None:intergenic |
| ACGAGAGTGCGAGGGTTACT+GGG | 0.515885 | 5.4:+4152879 | MS.gene22322:CDS |
| GTCGTAAAATCCCAATATGA+TGG | 0.518250 | 5.4:+4151843 | MS.gene22322:CDS |
| TCAAACCAGGTCATGTACAT+TGG | 0.518776 | 5.4:+4152764 | MS.gene22322:CDS |
| CGGGTCTACACACCTCGAAA+AGG | 0.518792 | 5.4:-4153165 | None:intergenic |
| CATGGGAAGCGACTTATAGT+AGG | 0.520691 | 5.4:-4159587 | None:intergenic |
| GATATTGTGCAACCTCCCTC+AGG | 0.523069 | 5.4:-4153115 | None:intergenic |
| GGTTAGGCTGCGAGCACTAG+TGG | 0.523379 | 5.4:-4158506 | None:intergenic |
| AATTCAGTTAGAGGCTGTTG+AGG | 0.529039 | 5.4:+4151791 | MS.gene22322:CDS |
| TAATTGTTCAACCAACTTGG+AGG | 0.530716 | 5.4:-4155204 | None:intergenic |
| TTACAACAAGCTTGAAGTGC+GGG | 0.531222 | 5.4:+4155616 | MS.gene22322:CDS |
| GTGAAAGATGGGAAAATAAG+TGG | 0.531333 | 5.4:+4153083 | MS.gene22322:CDS |
| AGAGGAAGATGTTACTTGCT+CGG | 0.532696 | 5.4:-4152825 | None:intergenic |
| CTGTTTGACATCTCAACAGC+AGG | 0.533413 | 5.4:-4155797 | None:intergenic |
| CTCATCAAAAGAGAAGAAGC+AGG | 0.539036 | 5.4:+4155175 | MS.gene22322:CDS |
| AATACAGTGAACATGAAAAG+AGG | 0.539565 | 5.4:+4157122 | MS.gene22322:CDS |
| CAGCTATGGGATGCTAGTAC+GGG | 0.540078 | 5.4:+4157085 | MS.gene22322:intron |
| TAATGCAGTTGAACATTTGG+CGG | 0.540996 | 5.4:+4152178 | MS.gene22322:CDS |
| TAATTCTATATGTGAAAGAT+GGG | 0.542817 | 5.4:+4153072 | MS.gene22322:CDS |
| GTGATGTTCATCGAGGAATA+CGG | 0.544130 | 5.4:+4152234 | MS.gene22322:CDS |
| TCTAGTGCTTGGGAGGATGT+TGG | 0.545873 | 5.4:+4152101 | MS.gene22322:CDS |
| AAATTATCGTAACAGATATG+AGG | 0.547870 | 5.4:+4152964 | MS.gene22322:CDS |
| ATCTTTATCTGGGTGATCTG+TGG | 0.548330 | 5.4:-4155486 | None:intergenic |
| TTTCCTATCAGAACATCCTA+AGG | 0.555337 | 5.4:+4153809 | MS.gene22322:CDS |
| GTTTCAAGTGTGTGCTGGAG+AGG | 0.558709 | 5.4:+4159685 | MS.gene22322:CDS |
| TCAGTCTCTTTACCAGAGAT+GGG | 0.559728 | 5.4:-4159643 | None:intergenic |
| TCATCAAACATCTACTGCCT+CGG | 0.561737 | 5.4:+4153136 | MS.gene22322:CDS |
| TTCGCAATCTTAGAAGTCCC+TGG | 0.562968 | 5.4:+4158353 | MS.gene22322:CDS |
| AATCAGAAACAAATGAAGAC+TGG | 0.563404 | 5.4:+4152470 | MS.gene22322:CDS |
| ACATAAACGCGATCAGAAGC+TGG | 0.563636 | 5.4:-4152338 | None:intergenic |
| CAAAGATGATATCCAAATCA+GGG | 0.570823 | 5.4:+4152258 | MS.gene22322:CDS |
| AAAAGTAAGCACTTTCAAGA+TGG | 0.572294 | 5.4:-4155427 | None:intergenic |
| TTGGAGATTGGGGACACTGT+GGG | 0.573052 | 5.4:-4155371 | None:intergenic |
| AAGGAGGGTGTAGGACCCGC+AGG | 0.573680 | 5.4:+4152212 | MS.gene22322:CDS |
| AAGTGGTATGCGAGTCCTGA+GGG | 0.573844 | 5.4:+4153100 | MS.gene22322:CDS |
| CTCAAGTTCAACATAAACAT+TGG | 0.574254 | 5.4:+4151991 | MS.gene22322:CDS |
| CATCTTCCTCTGTTGATATG+GGG | 0.576607 | 5.4:+4152837 | MS.gene22322:CDS |
| CTTGCACAAACCATCAAAGA+AGG | 0.577758 | 5.4:-4155585 | None:intergenic |
| CAAGCACTAGACGTAGCTGC+GGG | 0.585754 | 5.4:-4152089 | None:intergenic |
| AGTACAATCATCACTTCCAC+TGG | 0.586568 | 5.4:-4157188 | None:intergenic |
| AATCAGAATTGGGGTAAGAT+CGG | 0.586685 | 5.4:+4152404 | MS.gene22322:CDS |
| GAAGAGGCAGAGAGGAGGCA+TGG | 0.588049 | 5.4:+4155248 | MS.gene22322:CDS |
| AATTGGGGTAAGATCGGCGT+TGG | 0.590002 | 5.4:+4152410 | MS.gene22322:CDS |
| AGTCCCTGGTGTGTATTGGC+TGG | 0.591751 | 5.4:+4158367 | MS.gene22322:CDS |
| TGAGTAATTCGCAGATCCAA+AGG | 0.594338 | 5.4:-4158314 | None:intergenic |
| CCTCGATGAACATCACCTGC+GGG | 0.594373 | 5.4:-4152227 | None:intergenic |
| ATACAGTGAACATGAAAAGA+GGG | 0.594379 | 5.4:+4157123 | MS.gene22322:CDS |
| ACGGTGTAACATTGAGAGAA+TGG | 0.595622 | 5.4:+4152603 | MS.gene22322:CDS |
| TTCATTAGTATGTCCAGACA+GGG | 0.595786 | 5.4:-4158527 | None:intergenic |
| ATATTGGGTTGATTAGTAGT+GGG | 0.599244 | 5.4:+4152066 | MS.gene22322:CDS |
| TTTCAAGTGTGTGCTGGAGA+GGG | 0.601436 | 5.4:+4159686 | MS.gene22322:CDS |
| GCAGGGTGATGCCTCCAAGT+TGG | 0.602461 | 5.4:+4155193 | MS.gene22322:CDS |
| CAGATATAGAAGAGGCAGAG+AGG | 0.604924 | 5.4:+4155240 | MS.gene22322:CDS |
| ATTACAGGAGTTGTGTAATG+AGG | 0.606394 | 5.4:+4155091 | MS.gene22322:CDS |
| GGCGGCTGCGGAAAGTAAGG+AGG | 0.607941 | 5.4:+4152196 | MS.gene22322:CDS |
| GCTAGAAAATCGCATAGTGA+TGG | 0.608608 | 5.4:+4152149 | MS.gene22322:CDS |
| CTTGGAATCAGATATAGAAG+AGG | 0.610271 | 5.4:+4155232 | MS.gene22322:CDS |
| ATGCTCTCTAAGCTTTGACC+GGG | 0.612774 | 5.4:+4155682 | MS.gene22322:CDS |
| AGCTACGTCTAGTGCTTGGG+AGG | 0.615576 | 5.4:+4152094 | MS.gene22322:CDS |
| AAGCAAAGTAATCTTCATCC+CGG | 0.620471 | 5.4:-4155700 | None:intergenic |
| AGACTACGACGGTGTAGTGA+AGG | 0.621641 | 5.4:+4155877 | MS.gene22322:CDS |
| GAGATTGGGGACACTGTGGG+CGG | 0.622277 | 5.4:-4155368 | None:intergenic |
| CCAGCGCAAATCAGGCAAAG+TGG | 0.623535 | 5.4:+4152637 | MS.gene22322:CDS |
| TTGTGTGAAGTAATTGGCAT+GGG | 0.625244 | 5.4:-4159604 | None:intergenic |
| TCCAAAGGCAAGCAAATGAG+AGG | 0.625566 | 5.4:-4158299 | None:intergenic |
| GCGGAAAGTAAGGAGGGTGT+AGG | 0.626048 | 5.4:+4152203 | MS.gene22322:CDS |
| GTCTACACACCTCGAAAAGG+AGG | 0.626294 | 5.4:-4153162 | None:intergenic |
| GTGGATGACTCACACTCTCG+GGG | 0.627042 | 5.4:+4152695 | MS.gene22322:CDS |
| ATGGCCAGCCAATACACACC+AGG | 0.627800 | 5.4:-4158371 | None:intergenic |
| AAAATTTGACGAGAGTGCGA+GGG | 0.629892 | 5.4:+4152871 | MS.gene22322:CDS |
| TAGAGAGCAAAATCAGACAA+AGG | 0.631176 | 5.4:+4152367 | MS.gene22322:CDS |
| CCAAGCACTAGACGTAGCTG+CGG | 0.631837 | 5.4:-4152090 | None:intergenic |
| CACTTGAAACAAACTGACCA+TGG | 0.631982 | 5.4:-4159672 | None:intergenic |
| AGCTAATAACTCCGGAAACG+AGG | 0.632584 | 5.4:-4152129 | None:intergenic |
| TCCTCGATGAACATCACCTG+CGG | 0.634907 | 5.4:-4152228 | None:intergenic |
| CTTGTGTGAAGTAATTGGCA+TGG | 0.647108 | 5.4:-4159605 | None:intergenic |
| ATATAGAAGAGGCAGAGAGG+AGG | 0.647655 | 5.4:+4155243 | MS.gene22322:CDS |
| ATTGGAGATTGGGGACACTG+TGG | 0.648633 | 5.4:-4155372 | None:intergenic |
| TCATCAAAAGAGAAGAAGCA+GGG | 0.652591 | 5.4:+4155176 | MS.gene22322:CDS |
| CATAGCTTACAGCTTTACGA+TGG | 0.656922 | 5.4:-4158390 | None:intergenic |
| GCGGCTGCGGAAAGTAAGGA+GGG | 0.659078 | 5.4:+4152197 | MS.gene22322:CDS |
| TAAATTGCTACCATCAAACC+AGG | 0.660402 | 5.4:+4152751 | MS.gene22322:CDS |
| CTTGGACCCCATATCAACAG+AGG | 0.661094 | 5.4:-4152843 | None:intergenic |
| CTGCCAGCCAGAGGAAACCA+AGG | 0.661718 | 5.4:-4155282 | None:intergenic |
| TGCCCTACAAAATTTGCCAG+TGG | 0.662507 | 5.4:+4157172 | MS.gene22322:CDS |
| CATCATCACGTCCAACAACA+GGG | 0.666527 | 5.4:+4153868 | MS.gene22322:CDS |
| AGTGGATGAACCAACCGAAG+AGG | 0.670752 | 5.4:-4158488 | None:intergenic |
| TACACCGTCGTAGTCTGTAG+AGG | 0.672028 | 5.4:-4155870 | None:intergenic |
| TACAACAAGCTTGAAGTGCG+GGG | 0.673879 | 5.4:+4155617 | MS.gene22322:CDS |
| TCTAATGTTTCAGTGAACCG+TGG | 0.676680 | 5.4:+4152512 | MS.gene22322:CDS |
| GTAACCCAATGTACATGACC+TGG | 0.681232 | 5.4:-4152769 | None:intergenic |
| GTTACAACAAGCTTGAAGTG+CGG | 0.682697 | 5.4:+4155615 | MS.gene22322:CDS |
| GCTGATGGATATATTGCATG+CGG | 0.691894 | 5.4:+4159211 | MS.gene22322:CDS |
| GTAATTGTTCTGCCAGCCAG+AGG | 0.692765 | 5.4:-4155291 | None:intergenic |
| GGTGATGCGAATCAGAATTG+GGG | 0.694044 | 5.4:+4152395 | MS.gene22322:CDS |
| ATGATTGTACTGTCAAGCTG+TGG | 0.694220 | 5.4:+4157200 | MS.gene22322:CDS |
| CAAAATCAGACAAAGGCAGG+CGG | 0.694575 | 5.4:+4152374 | MS.gene22322:CDS |
| TAATAACTCCGGAAACGAGG+TGG | 0.696527 | 5.4:-4152126 | None:intergenic |
| TGTTCTGATAGGAAAGCTGG+AGG | 0.696572 | 5.4:-4153801 | None:intergenic |
| TGACCTGAATTAGATACACG+AGG | 0.698186 | 5.4:-4153034 | None:intergenic |
| GACCTGAATTAGATACACGA+GGG | 0.702403 | 5.4:-4153033 | None:intergenic |
| TGGTATGCGAGTCCTGAGGG+AGG | 0.704127 | 5.4:+4153103 | MS.gene22322:CDS |
| TAAGTGGTATGCGAGTCCTG+AGG | 0.705242 | 5.4:+4153099 | MS.gene22322:CDS |
| TGGCCAGCCAATACACACCA+GGG | 0.709578 | 5.4:-4158370 | None:intergenic |
| TCTAGCACGACTGAATGTGA+CGG | 0.713007 | 5.4:+4152584 | MS.gene22322:CDS |
| CCCGCAGGTGATGTTCATCG+AGG | 0.729473 | 5.4:+4152227 | MS.gene22322:CDS |
| CCTCGAAAAGGAGGACACCG+AGG | 0.731822 | 5.4:-4153153 | None:intergenic |
| CTTGCCTCTACAGACTACGA+CGG | 0.736845 | 5.4:+4155866 | MS.gene22322:CDS |
| CAAGCTGTGGAGCATCAGTG+AGG | 0.737737 | 5.4:+4157213 | MS.gene22322:CDS |
| AAAGAGACTGATGATGACCA+TGG | 0.778100 | 5.4:+4159655 | MS.gene22322:CDS |
| ATGCGGTTCAGAATCAAACG+AGG | 0.781275 | 5.4:+4159228 | MS.gene22322:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 4151765 | 4159771 | 4151765 | ID=MS.gene22322 |
| chr5.4 | mRNA | 4151765 | 4159771 | 4151765 | ID=MS.gene22322.t1;Parent=MS.gene22322 |
| chr5.4 | exon | 4151765 | 4153174 | 4151765 | ID=MS.gene22322.t1.exon1;Parent=MS.gene22322.t1 |
| chr5.4 | CDS | 4151765 | 4153174 | 4151765 | ID=cds.MS.gene22322.t1;Parent=MS.gene22322.t1 |
| chr5.4 | exon | 4153732 | 4153889 | 4153732 | ID=MS.gene22322.t1.exon2;Parent=MS.gene22322.t1 |
| chr5.4 | CDS | 4153732 | 4153889 | 4153732 | ID=cds.MS.gene22322.t1;Parent=MS.gene22322.t1 |
| chr5.4 | exon | 4155061 | 4155898 | 4155061 | ID=MS.gene22322.t1.exon3;Parent=MS.gene22322.t1 |
| chr5.4 | CDS | 4155061 | 4155898 | 4155061 | ID=cds.MS.gene22322.t1;Parent=MS.gene22322.t1 |
| chr5.4 | exon | 4157088 | 4157234 | 4157088 | ID=MS.gene22322.t1.exon4;Parent=MS.gene22322.t1 |
| chr5.4 | CDS | 4157088 | 4157234 | 4157088 | ID=cds.MS.gene22322.t1;Parent=MS.gene22322.t1 |
| chr5.4 | exon | 4158235 | 4158552 | 4158235 | ID=MS.gene22322.t1.exon5;Parent=MS.gene22322.t1 |
| chr5.4 | CDS | 4158235 | 4158552 | 4158235 | ID=cds.MS.gene22322.t1;Parent=MS.gene22322.t1 |
| chr5.4 | exon | 4159190 | 4159249 | 4159190 | ID=MS.gene22322.t1.exon6;Parent=MS.gene22322.t1 |
| chr5.4 | CDS | 4159190 | 4159249 | 4159190 | ID=cds.MS.gene22322.t1;Parent=MS.gene22322.t1 |
| chr5.4 | exon | 4159580 | 4159771 | 4159580 | ID=MS.gene22322.t1.exon7;Parent=MS.gene22322.t1 |
| chr5.4 | CDS | 4159580 | 4159771 | 4159580 | ID=cds.MS.gene22322.t1;Parent=MS.gene22322.t1 |
| Gene Sequence |
| Protein sequence |
