AlfalfaGEDB | MS.gene24102

M. sativa cultivar XinJiangDaYe / MS.gene24102

KEGG: -
GO: -
PFAM: PF05347.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24102.t1	XP_013466199.1	94.2	137	7	1	1	137	1	136	1.60E-51	212.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24102.t1	I3S747	94.2	137	7	1	1	137	1	136	1.2e-51	212.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049166	MS.gene24102	0.808308	3.30E-50	-1.69E-46
MS.gene04984	MS.gene24102	0.813171	2.93E-51	-1.69E-46
MS.gene049887	MS.gene24102	0.82549	4.56E-54	-1.69E-46
MS.gene050114	MS.gene24102	0.805079	1.59E-49	-1.69E-46
MS.gene050317	MS.gene24102	0.835402	1.71E-56	-1.69E-46
MS.gene050815	MS.gene24102	0.805761	1.14E-49	-1.69E-46
MS.gene050838	MS.gene24102	0.822824	1.93E-53	-1.69E-46
MS.gene051003	MS.gene24102	0.808851	2.53E-50	-1.69E-46
MS.gene051245	MS.gene24102	0.807111	5.93E-50	-1.69E-46
MS.gene051757	MS.gene24102	0.837865	4.04E-57	-1.69E-46
MS.gene052397	MS.gene24102	0.800753	1.24E-48	-1.69E-46
MS.gene053109	MS.gene24102	0.814761	1.31E-51	-1.69E-46
MS.gene053381	MS.gene24102	0.800765	1.24E-48	-1.69E-46
MS.gene054823	MS.gene24102	0.801729	7.85E-49	-1.69E-46
MS.gene055311	MS.gene24102	0.827651	1.39E-54	-1.69E-46
MS.gene055313	MS.gene24102	0.80508	1.59E-49	-1.69E-46
MS.gene056181	MS.gene24102	0.827045	1.94E-54	-1.69E-46
MS.gene056432	MS.gene24102	0.82316	1.61E-53	-1.69E-46
MS.gene056760	MS.gene24102	0.806402	8.37E-50	-1.69E-46
MS.gene057057	MS.gene24102	0.816384	5.69E-52	-1.69E-46
MS.gene057140	MS.gene24102	0.801199	1.01E-48	-1.69E-46
MS.gene057234	MS.gene24102	0.806565	7.73E-50	-1.69E-46
MS.gene058054	MS.gene24102	0.801484	8.81E-49	-1.69E-46
MS.gene058055	MS.gene24102	0.805347	1.39E-49	-1.69E-46
MS.gene058638	MS.gene24102	0.800608	1.33E-48	-1.69E-46
MS.gene058639	MS.gene24102	0.821331	4.27E-53	-1.69E-46
MS.gene058646	MS.gene24102	0.821837	3.26E-53	-1.69E-46
MS.gene058654	MS.gene24102	0.825997	3.45E-54	-1.69E-46
MS.gene058906	MS.gene24102	0.816048	6.76E-52	-1.69E-46
MS.gene059171	MS.gene24102	0.820982	5.14E-53	-1.69E-46
MS.gene059713	MS.gene24102	0.816977	4.19E-52	-1.69E-46
MS.gene06010	MS.gene24102	0.823212	1.56E-53	-1.69E-46
MS.gene060463	MS.gene24102	0.801399	9.17E-49	-1.69E-46
MS.gene060465	MS.gene24102	0.803794	2.94E-49	-1.69E-46
MS.gene060702	MS.gene24102	0.8244	8.24E-54	-1.69E-46
MS.gene060869	MS.gene24102	0.848788	4.90E-60	-1.69E-46
MS.gene060870	MS.gene24102	0.85537	6.60E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24102.t1	MTR_1g026078	90.625	160	6	3	1	152	1	159	6.38e-94	269

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24102.t1	AT1G76060	64.430	149	43	4	4	144	8	154	1.63e-57	176

Find 32 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGACACCGTGGAAGAGCTTC+TGG	0.320024	1.2:+240684	MS.gene24102:CDS
ATTGTCACTCGGTTGCTTAT+CGG	0.353596	1.2:+240937	MS.gene24102:CDS
CCGCCACGCGCAACCGTTTG+CGG	0.355220	1.2:-240644	None:intergenic
ACGATGCCAAAGGCGTTCTA+TGG	0.381120	1.2:+240848	MS.gene24102:CDS
GAGGAAAATGGCGAAGCCTT+TGG	0.391236	1.2:+240599	MS.gene24102:CDS
CATTGCTTCTCGGCGCGTGC+TGG	0.391282	1.2:-240779	None:intergenic
TGTAGAGACTCATTGCTTCT+CGG	0.396933	1.2:-240789	None:intergenic
AAGGATCGTCGCGGTGGCAG+TGG	0.428548	1.2:+241012	MS.gene24102:CDS
AGAAGCTCTTCCACGGTGTC+AGG	0.435099	1.2:-240682	None:intergenic
AAGAAGAAGAGTTGCTGAAT+CGG	0.460022	1.2:+240746	MS.gene24102:CDS
ATCACGCCATAGAACGCCTT+TGG	0.477887	1.2:-240854	None:intergenic
TGCGAGGTTTGAGACGGATC+CGG	0.482393	1.2:+240912	MS.gene24102:CDS
AGCAACCGAGTGACAATCTC+CGG	0.496816	1.2:-240931	None:intergenic
GATCGTCGCGGTGGCAGTGG+CGG	0.542143	1.2:+241015	MS.gene24102:CDS
TTCACATGGCACGATGCCAA+AGG	0.565614	1.2:+240838	MS.gene24102:CDS
GTTTCAGATTCGAATTCCAA+AGG	0.567422	1.2:-240615	None:intergenic
CCGTTCCAGAAGCTCTTCCA+CGG	0.587827	1.2:-240689	None:intergenic
TGAGCTTGCGAGGTTTGAGA+CGG	0.591164	1.2:+240906	MS.gene24102:CDS
CCGCAAACGGTTGCGCGTGG+CGG	0.594281	1.2:+240644	MS.gene24102:CDS
GTCACTCGGTTGCTTATCGG+TGG	0.599413	1.2:+240940	MS.gene24102:CDS
GTAAAGAGTTTGAGCTTGCG+AGG	0.613271	1.2:+240896	MS.gene24102:CDS
CCGTGGAAGAGCTTCTGGAA+CGG	0.622869	1.2:+240689	MS.gene24102:CDS
AAACCGCAAACGGTTGCGCG+TGG	0.625582	1.2:+240641	MS.gene24102:CDS
GTTGCTTATCGGTGGACGTG+AGG	0.631369	1.2:+240948	MS.gene24102:CDS
CAACGCTATTGATAAGCTTG+CGG	0.647873	1.2:+240978	MS.gene24102:CDS
AAGAAGAGTTGCTGAATCGG+CGG	0.650336	1.2:+240749	MS.gene24102:CDS
ATTCAGAAGGATCGTCGCGG+TGG	0.657849	1.2:+241006	MS.gene24102:CDS
GCTTGCGGAACAGATTCAGA+AGG	0.661253	1.2:+240993	MS.gene24102:CDS
CGGATCCGGAGATTGTCACT+CGG	0.686306	1.2:+240926	MS.gene24102:CDS
ACACGACGGTCCTGACACCG+TGG	0.698737	1.2:+240672	MS.gene24102:CDS
GCGTGGCGGTTATTACACGA+CGG	0.720396	1.2:+240658	MS.gene24102:CDS
CAGATTCAGAAGGATCGTCG+CGG	0.756987	1.2:+241003	MS.gene24102:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGAAGAAGAGTTGCTGAAT+CGG	+	chr1.2:240746-240765	MS.gene24102:CDS	35.0%
	GTTTCAGATTCGAATTCCAA+AGG	-	chr1.2:240618-240637	None:intergenic	35.0%
	ATTGTCACTCGGTTGCTTAT+CGG	+	chr1.2:240937-240956	MS.gene24102:CDS	40.0%
	CAACGCTATTGATAAGCTTG+CGG	+	chr1.2:240978-240997	MS.gene24102:CDS	40.0%
	CTGAAACCAAAAACCGCAAA+CGG	+	chr1.2:240631-240650	MS.gene24102:CDS	40.0%
	TGTAGAGACTCATTGCTTCT+CGG	-	chr1.2:240792-240811	None:intergenic	40.0%
!	GTGCTTCACGATTTTTCACA+TGG	+	chr1.2:240824-240843	MS.gene24102:CDS	40.0%
	AAGAAGAGTTGCTGAATCGG+CGG	+	chr1.2:240749-240768	MS.gene24102:CDS	45.0%
	GTAAAGAGTTTGAGCTTGCG+AGG	+	chr1.2:240896-240915	MS.gene24102:CDS	45.0%
	AGCAACCGAGTGACAATCTC+CGG	-	chr1.2:240934-240953	None:intergenic	50.0%
	ATCACGCCATAGAACGCCTT+TGG	-	chr1.2:240857-240876	None:intergenic	50.0%
	CAGATTCAGAAGGATCGTCG+CGG	+	chr1.2:241003-241022	MS.gene24102:CDS	50.0%
	GAGGAAAATGGCGAAGCCTT+TGG	+	chr1.2:240599-240618	MS.gene24102:CDS	50.0%
	GCTTGCGGAACAGATTCAGA+AGG	+	chr1.2:240993-241012	MS.gene24102:CDS	50.0%
	TGAGCTTGCGAGGTTTGAGA+CGG	+	chr1.2:240906-240925	MS.gene24102:CDS	50.0%
	TTCACATGGCACGATGCCAA+AGG	+	chr1.2:240838-240857	MS.gene24102:CDS	50.0%
!!	ACGATGCCAAAGGCGTTCTA+TGG	+	chr1.2:240848-240867	MS.gene24102:CDS	50.0%
	AGAAGCTCTTCCACGGTGTC+AGG	-	chr1.2:240685-240704	None:intergenic	55.0%
	ATTCAGAAGGATCGTCGCGG+TGG	+	chr1.2:241006-241025	MS.gene24102:CDS	55.0%
	CCGTTCCAGAAGCTCTTCCA+CGG	-	chr1.2:240692-240711	None:intergenic	55.0%
	CGGATCCGGAGATTGTCACT+CGG	+	chr1.2:240926-240945	MS.gene24102:CDS	55.0%
	GCGTGGCGGTTATTACACGA+CGG	+	chr1.2:240658-240677	MS.gene24102:CDS	55.0%
	GTCACTCGGTTGCTTATCGG+TGG	+	chr1.2:240940-240959	MS.gene24102:CDS	55.0%
	GTTGCTTATCGGTGGACGTG+AGG	+	chr1.2:240948-240967	MS.gene24102:CDS	55.0%
	TGACACCGTGGAAGAGCTTC+TGG	+	chr1.2:240684-240703	MS.gene24102:CDS	55.0%
!	CCGTGGAAGAGCTTCTGGAA+CGG	+	chr1.2:240689-240708	MS.gene24102:CDS	55.0%
!	GCGCAACCGTTTGCGGTTTT+TGG	-	chr1.2:240640-240659	None:intergenic	55.0%
!	TGCGAGGTTTGAGACGGATC+CGG	+	chr1.2:240912-240931	MS.gene24102:CDS	55.0%
	AAACCGCAAACGGTTGCGCG+TGG	+	chr1.2:240641-240660	MS.gene24102:CDS	60.0%
	AAGGATCGTCGCGGTGGCAG+TGG	+	chr1.2:241012-241031	MS.gene24102:CDS	65.0%
	ACACGACGGTCCTGACACCG+TGG	+	chr1.2:240672-240691	MS.gene24102:CDS	65.0%
	CATTGCTTCTCGGCGCGTGC+TGG	-	chr1.2:240782-240801	None:intergenic	65.0%
	CCGCAAACGGTTGCGCGTGG+CGG	+	chr1.2:240644-240663	MS.gene24102:CDS	70.0%
	CCGCCACGCGCAACCGTTTG+CGG	-	chr1.2:240647-240666	None:intergenic	70.0%
	GATCGTCGCGGTGGCAGTGG+CGG	+	chr1.2:241015-241034	MS.gene24102:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	240595	241053	240595	ID=MS.gene24102
chr1.2	mRNA	240595	241053	240595	ID=MS.gene24102.t1;Parent=MS.gene24102
chr1.2	exon	240595	241053	240595	ID=MS.gene24102.t1.exon1;Parent=MS.gene24102.t1
chr1.2	CDS	240595	241053	240595	ID=cds.MS.gene24102.t1;Parent=MS.gene24102.t1

Gene Sequence

>MS.gene24102

ATGAGAGGAAAATGGCGAAGCCTTTGGAATTCGAATCTGAAACCAAAAACCGCAAACGGTTGCGCGTGGCGGTTATTACACGACGGTCCTGACACCGTGGAAGAGCTTCTGGAACGGCATCTAGTGAAGAAAGTTATCAACGATGATGATGAAGAAGAAGAGTTGCTGAATCGGCGGCGACTCACCAGCACGCGCCGAGAAGCAATGAGTCTCTACAGAGACATTCTTCGTGCTTCACGATTTTTCACATGGCACGATGCCAAAGGCGTTCTATGGCGTGATCTTCTTAGAGATAGCGCTCGTAAAGAGTTTGAGCTTGCGAGGTTTGAGACGGATCCGGAGATTGTCACTCGGTTGCTTATCGGTGGACGTGAGGCTGTCAACAACGCTATTGATAAGCTTGCGGAACAGATTCAGAAGGATCGTCGCGGTGGCAGTGGCGGTTTTGATCAAACGTGA

Protein sequence

>MS.gene24102.t1

MRGKWRSLWNSNLKPKTANGCAWRLLHDGPDTVEELLERHLVKKVINDDDEEEELLNRRRLTSTRREAMSLYRDILRASRFFTWHDAKGVLWRDLLRDSARKEFELARFETDPEIVTRLLIGGREAVNNAIDKLAEQIQKDRRGGSGGFDQT