Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24885.t1 | RHN79081.1 | 94.5 | 145 | 8 | 0 | 1 | 145 | 464 | 608 | 3.30E-73 | 284.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24885.t1 | O48534 | 70.5 | 139 | 41 | 0 | 1 | 139 | 2030 | 2168 | 9.0e-56 | 217.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24885.t1 | A0A396JQU6 | 94.5 | 145 | 8 | 0 | 1 | 145 | 464 | 608 | 2.3e-73 | 284.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049404 | MS.gene24885 | 0.803495 | 3.39E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene24885 | 0.820007 | 8.61E-53 | -1.69E-46 |
| MS.gene050611 | MS.gene24885 | 0.815177 | 1.06E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene24885 | 0.813026 | 3.15E-51 | -1.69E-46 |
| MS.gene051594 | MS.gene24885 | 0.803769 | 2.98E-49 | -1.69E-46 |
| MS.gene051957 | MS.gene24885 | 0.814082 | 1.85E-51 | -1.69E-46 |
| MS.gene052190 | MS.gene24885 | 0.803179 | 3.94E-49 | -1.69E-46 |
| MS.gene052447 | MS.gene24885 | 0.805275 | 1.44E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene24885 | 0.800404 | 1.46E-48 | -1.69E-46 |
| MS.gene053154 | MS.gene24885 | 0.800865 | 1.18E-48 | -1.69E-46 |
| MS.gene053883 | MS.gene24885 | 0.811475 | 6.87E-51 | -1.69E-46 |
| MS.gene053974 | MS.gene24885 | 0.843205 | 1.62E-58 | -1.69E-46 |
| MS.gene053998 | MS.gene24885 | 0.805394 | 1.36E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene24885 | 0.804826 | 1.79E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene24885 | 0.80179 | 7.63E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene24885 | 0.816366 | 5.74E-52 | -1.69E-46 |
| MS.gene056859 | MS.gene24885 | 0.800043 | 1.73E-48 | -1.69E-46 |
| MS.gene057021 | MS.gene24885 | 0.827488 | 1.52E-54 | -1.69E-46 |
| MS.gene057060 | MS.gene24885 | 0.812638 | 3.83E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene24885 | 0.811517 | 6.73E-51 | -1.69E-46 |
| MS.gene058294 | MS.gene24885 | 0.800298 | 1.54E-48 | -1.69E-46 |
| MS.gene058515 | MS.gene24885 | 0.811211 | 7.84E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene24885 | 0.815711 | 8.04E-52 | -1.69E-46 |
| MS.gene059015 | MS.gene24885 | 0.808722 | 2.69E-50 | -1.69E-46 |
| MS.gene059458 | MS.gene24885 | 0.821497 | 3.91E-53 | -1.69E-46 |
| MS.gene059459 | MS.gene24885 | 0.812452 | 4.21E-51 | -1.69E-46 |
| MS.gene059584 | MS.gene24885 | 0.822398 | 2.42E-53 | -1.69E-46 |
| MS.gene060137 | MS.gene24885 | -0.806479 | 8.06E-50 | -1.69E-46 |
| MS.gene060521 | MS.gene24885 | 0.817661 | 2.94E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24885.t1 | MTR_2g064740 | 86.331 | 139 | 19 | 0 | 1 | 139 | 2048 | 2186 | 7.55e-82 | 262 |
| MS.gene24885.t1 | MTR_4g008780 | 83.448 | 145 | 6 | 1 | 1 | 145 | 764 | 890 | 4.22e-78 | 248 |
| MS.gene24885.t1 | MTR_4g011450 | 82.069 | 145 | 6 | 1 | 1 | 145 | 1948 | 2072 | 2.05e-75 | 244 |
| MS.gene24885.t1 | MTR_4g011450 | 82.069 | 145 | 6 | 1 | 1 | 145 | 1975 | 2099 | 2.11e-75 | 244 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24885.t1 | AT2G42270 | 70.504 | 139 | 41 | 0 | 1 | 139 | 2030 | 2168 | 1.19e-68 | 224 |
| MS.gene24885.t1 | AT1G20960 | 68.571 | 140 | 44 | 0 | 1 | 140 | 2029 | 2168 | 1.60e-66 | 218 |
| MS.gene24885.t1 | AT1G20960 | 68.571 | 140 | 44 | 0 | 1 | 140 | 2029 | 2168 | 1.60e-66 | 218 |
| MS.gene24885.t1 | AT3G28870 | 64.583 | 48 | 17 | 0 | 92 | 139 | 304 | 351 | 4.56e-15 | 70.9 |
| MS.gene24885.t1 | AT5G61140 | 32.759 | 116 | 75 | 2 | 13 | 128 | 2038 | 2150 | 9.94e-15 | 70.5 |
| MS.gene24885.t1 | AT5G61140 | 32.759 | 116 | 75 | 2 | 13 | 128 | 2027 | 2139 | 1.06e-14 | 70.5 |
Find 34 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGCCAAATTCATACTTAT+TGG | 0.152145 | 4.1:+84187640 | None:intergenic |
| CATCCCTCCTCCTTAGCTTT+TGG | 0.188606 | 4.1:-84187824 | None:intergenic |
| TGCAAGTAACGAGTTGGTTT+TGG | 0.306839 | 4.1:-84187867 | None:intergenic |
| ATGTCCAATAAGTATGAATT+TGG | 0.325930 | 4.1:-84187644 | None:intergenic |
| GAGGGATGGTGGCTTGTTGT+TGG | 0.348617 | 4.1:+84187839 | MS.gene24885:CDS |
| TAGTTTCACTGTTGATGTTA+AGG | 0.374452 | 4.1:+84188018 | MS.gene24885:CDS |
| TCATACGACAAATCAACATT+AGG | 0.425562 | 4.1:-84187689 | None:intergenic |
| TTTGCTGCACCTGCTGATGC+TGG | 0.442977 | 4.1:+84187935 | MS.gene24885:CDS |
| TTTCATGTGCGACTCATACA+TGG | 0.461520 | 4.1:+84187979 | MS.gene24885:CDS |
| AGGTGCAGCAAACTGCAGTT+TGG | 0.462926 | 4.1:-84187924 | None:intergenic |
| TACTGCACAATGACAGTGTC+CGG | 0.474800 | 4.1:+84187714 | MS.gene24885:CDS |
| GGATACCTAGGAGCATCAAC+AGG | 0.495924 | 4.1:-84187803 | None:intergenic |
| GATTTCTTTCCAGCATCAGC+AGG | 0.500461 | 4.1:-84187944 | None:intergenic |
| CTCGTTACTTGCAATTAAGC+GGG | 0.507205 | 4.1:+84187877 | MS.gene24885:CDS |
| CTTAATTGCAAGTAACGAGT+TGG | 0.531343 | 4.1:-84187873 | None:intergenic |
| TTCATGTGCGACTCATACAT+GGG | 0.543376 | 4.1:+84187980 | MS.gene24885:CDS |
| TTGTTATATCATCACCAGTC+CGG | 0.546313 | 4.1:-84187733 | None:intergenic |
| TATAACAATATGTGTCACTC+TGG | 0.554694 | 4.1:+84187748 | MS.gene24885:CDS |
| TAGGACCTGTTGATGCTCCT+AGG | 0.567274 | 4.1:+84187798 | MS.gene24885:CDS |
| GTATCCAAAAGCTAAGGAGG+AGG | 0.574559 | 4.1:+84187820 | MS.gene24885:CDS |
| TCCTAGGTATCCAAAAGCTA+AGG | 0.587586 | 4.1:+84187814 | MS.gene24885:CDS |
| ACTCGTTACTTGCAATTAAG+CGG | 0.599217 | 4.1:+84187876 | MS.gene24885:CDS |
| AATATGTGTCACTCTGGAGA+GGG | 0.599575 | 4.1:+84187754 | MS.gene24885:CDS |
| CACAATGACAGTGTCCGGAC+TGG | 0.599754 | 4.1:+84187719 | MS.gene24885:CDS |
| TAGGTATCCAAAAGCTAAGG+AGG | 0.605710 | 4.1:+84187817 | MS.gene24885:CDS |
| ATCGACGGTAAGACAGAAGT+AGG | 0.607178 | 4.1:+84187779 | MS.gene24885:CDS |
| CAATATGTGTCACTCTGGAG+AGG | 0.618178 | 4.1:+84187753 | MS.gene24885:CDS |
| TATCCAAAAGCTAAGGAGGA+GGG | 0.622215 | 4.1:+84187821 | MS.gene24885:CDS |
| CAAAAGCTAAGGAGGAGGGA+TGG | 0.631038 | 4.1:+84187825 | MS.gene24885:CDS |
| AAGCTAAGGAGGAGGGATGG+TGG | 0.635031 | 4.1:+84187828 | MS.gene24885:CDS |
| TTAAGCGGGTTTCCTTGCAG+CGG | 0.683267 | 4.1:+84187891 | MS.gene24885:CDS |
| GTTGATGTTAAGGAAGCAGA+TGG | 0.692437 | 4.1:+84188028 | MS.gene24885:CDS |
| GATGTTAAGGAAGCAGATGG+TGG | 0.714823 | 4.1:+84188031 | MS.gene24885:CDS |
| ACTCTGGAGAGGGATATCGA+CGG | 0.757543 | 4.1:+84187764 | MS.gene24885:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGTCCAATAAGTATGAATT+TGG | - | chr4.1:84187647-84187666 | None:intergenic | 25.0% |
| TAGTTTCACTGTTGATGTTA+AGG | + | chr4.1:84188018-84188037 | MS.gene24885:CDS | 30.0% | |
| TCATACGACAAATCAACATT+AGG | - | chr4.1:84187692-84187711 | None:intergenic | 30.0% | |
| ! | TATAACAATATGTGTCACTC+TGG | + | chr4.1:84187748-84187767 | MS.gene24885:CDS | 30.0% |
| ACTCGTTACTTGCAATTAAG+CGG | + | chr4.1:84187876-84187895 | MS.gene24885:CDS | 35.0% | |
| CTTAATTGCAAGTAACGAGT+TGG | - | chr4.1:84187876-84187895 | None:intergenic | 35.0% | |
| TTGTTATATCATCACCAGTC+CGG | - | chr4.1:84187736-84187755 | None:intergenic | 35.0% | |
| CTCGTTACTTGCAATTAAGC+GGG | + | chr4.1:84187877-84187896 | MS.gene24885:CDS | 40.0% | |
| GTTGATGTTAAGGAAGCAGA+TGG | + | chr4.1:84188028-84188047 | MS.gene24885:CDS | 40.0% | |
| TAGGTATCCAAAAGCTAAGG+AGG | + | chr4.1:84187817-84187836 | MS.gene24885:CDS | 40.0% | |
| TATCCAAAAGCTAAGGAGGA+GGG | + | chr4.1:84187821-84187840 | MS.gene24885:CDS | 40.0% | |
| TCCTAGGTATCCAAAAGCTA+AGG | + | chr4.1:84187814-84187833 | MS.gene24885:CDS | 40.0% | |
| TTCATGTGCGACTCATACAT+GGG | + | chr4.1:84187980-84187999 | MS.gene24885:CDS | 40.0% | |
| TTTCATGTGCGACTCATACA+TGG | + | chr4.1:84187979-84187998 | MS.gene24885:CDS | 40.0% | |
| ! | AATATGTGTCACTCTGGAGA+GGG | + | chr4.1:84187754-84187773 | MS.gene24885:CDS | 40.0% |
| !! | TCCTTAGCTTTTGGATACCT+AGG | - | chr4.1:84187818-84187837 | None:intergenic | 40.0% |
| !!! | TGCAAGTAACGAGTTGGTTT+TGG | - | chr4.1:84187870-84187889 | None:intergenic | 40.0% |
| ATCGACGGTAAGACAGAAGT+AGG | + | chr4.1:84187779-84187798 | MS.gene24885:CDS | 45.0% | |
| GATGTTAAGGAAGCAGATGG+TGG | + | chr4.1:84188031-84188050 | MS.gene24885:CDS | 45.0% | |
| GATTTCTTTCCAGCATCAGC+AGG | - | chr4.1:84187947-84187966 | None:intergenic | 45.0% | |
| GTATCCAAAAGCTAAGGAGG+AGG | + | chr4.1:84187820-84187839 | MS.gene24885:CDS | 45.0% | |
| TACTGCACAATGACAGTGTC+CGG | + | chr4.1:84187714-84187733 | MS.gene24885:CDS | 45.0% | |
| ! | CAATATGTGTCACTCTGGAG+AGG | + | chr4.1:84187753-84187772 | MS.gene24885:CDS | 45.0% |
| !!! | GGCTTTTAATTTCCGCTGCA+AGG | - | chr4.1:84187906-84187925 | None:intergenic | 45.0% |
| AGGTGCAGCAAACTGCAGTT+TGG | - | chr4.1:84187927-84187946 | None:intergenic | 50.0% | |
| CAAAAGCTAAGGAGGAGGGA+TGG | + | chr4.1:84187825-84187844 | MS.gene24885:CDS | 50.0% | |
| TAGGACCTGTTGATGCTCCT+AGG | + | chr4.1:84187798-84187817 | MS.gene24885:CDS | 50.0% | |
| ! | ACTCTGGAGAGGGATATCGA+CGG | + | chr4.1:84187764-84187783 | MS.gene24885:CDS | 50.0% |
| ! | TTAAGCGGGTTTCCTTGCAG+CGG | + | chr4.1:84187891-84187910 | MS.gene24885:CDS | 50.0% |
| !! | CATCCCTCCTCCTTAGCTTT+TGG | - | chr4.1:84187827-84187846 | None:intergenic | 50.0% |
| !! | GGATACCTAGGAGCATCAAC+AGG | - | chr4.1:84187806-84187825 | None:intergenic | 50.0% |
| AAGCTAAGGAGGAGGGATGG+TGG | + | chr4.1:84187828-84187847 | MS.gene24885:CDS | 55.0% | |
| CACAATGACAGTGTCCGGAC+TGG | + | chr4.1:84187719-84187738 | MS.gene24885:CDS | 55.0% | |
| TTTGCTGCACCTGCTGATGC+TGG | + | chr4.1:84187935-84187954 | MS.gene24885:CDS | 55.0% | |
| !! | GAGGGATGGTGGCTTGTTGT+TGG | + | chr4.1:84187839-84187858 | MS.gene24885:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 84187641 | 84188078 | 84187641 | ID=MS.gene24885 |
| chr4.1 | mRNA | 84187641 | 84188078 | 84187641 | ID=MS.gene24885.t1;Parent=MS.gene24885 |
| chr4.1 | exon | 84187641 | 84188078 | 84187641 | ID=MS.gene24885.t1.exon1;Parent=MS.gene24885.t1 |
| chr4.1 | CDS | 84187641 | 84188078 | 84187641 | ID=cds.MS.gene24885.t1;Parent=MS.gene24885.t1 |
| Gene Sequence |
| Protein sequence |
