Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26056.t1 | AFK35673.1 | 80 | 80 | 4 | 1 | 1 | 68 | 13 | 92 | 1.40E-24 | 122.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26056.t1 | Q43207 | 65.3 | 75 | 13 | 2 | 6 | 68 | 21 | 94 | 2.7e-16 | 86.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26056.t1 | G7LF61 | 80.0 | 80 | 4 | 1 | 1 | 68 | 13 | 92 | 1.0e-24 | 122.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene26056 | 0.824559 | 7.56E-54 | -1.69E-46 |
| MS.gene049587 | MS.gene26056 | 0.835913 | 1.27E-56 | -1.69E-46 |
| MS.gene049701 | MS.gene26056 | 0.801115 | 1.05E-48 | -1.69E-46 |
| MS.gene049853 | MS.gene26056 | 0.841733 | 3.97E-58 | -1.69E-46 |
| MS.gene050611 | MS.gene26056 | 0.84572 | 3.40E-59 | -1.69E-46 |
| MS.gene051710 | MS.gene26056 | 0.804968 | 1.67E-49 | -1.69E-46 |
| MS.gene053512 | MS.gene26056 | 0.811556 | 6.60E-51 | -1.69E-46 |
| MS.gene053974 | MS.gene26056 | 0.836544 | 8.79E-57 | -1.69E-46 |
| MS.gene054174 | MS.gene26056 | 0.82079 | 5.70E-53 | -1.69E-46 |
| MS.gene054175 | MS.gene26056 | 0.825613 | 4.26E-54 | -1.69E-46 |
| MS.gene055017 | MS.gene26056 | 0.812784 | 3.56E-51 | -1.69E-46 |
| MS.gene055056 | MS.gene26056 | 0.830966 | 2.18E-55 | -1.69E-46 |
| MS.gene055058 | MS.gene26056 | 0.816946 | 4.25E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene26056 | 0.85265 | 4.02E-61 | -1.69E-46 |
| MS.gene059276 | MS.gene26056 | 0.865518 | 5.57E-65 | -1.69E-46 |
| MS.gene061072 | MS.gene26056 | 0.813478 | 2.51E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26056.t1 | MTR_8g012340 | 77.500 | 80 | 6 | 1 | 1 | 68 | 13 | 92 | 1.08e-23 | 95.1 |
| MS.gene26056.t1 | MTR_8g080980 | 56.667 | 90 | 17 | 2 | 1 | 68 | 1 | 90 | 5.87e-22 | 90.1 |
| MS.gene26056.t1 | MTR_7g053470 | 53.488 | 86 | 22 | 2 | 1 | 68 | 1 | 86 | 2.48e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26056.t1 | AT5G48570 | 62.500 | 72 | 15 | 1 | 10 | 69 | 29 | 100 | 1.44e-19 | 83.2 |
| MS.gene26056.t1 | AT3G25230 | 69.231 | 65 | 7 | 2 | 16 | 68 | 28 | 91 | 8.61e-18 | 78.2 |
| MS.gene26056.t1 | AT3G25230 | 69.231 | 65 | 7 | 2 | 16 | 68 | 28 | 91 | 1.02e-17 | 78.2 |
Find 29 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATACTAGAACGTTGCTTGA+TGG | 0.359676 | 7.4:+54160462 | MS.gene26056:CDS |
| ACTACTTTCAATTTCACTCT+TGG | 0.361943 | 7.4:+54160516 | MS.gene26056:CDS |
| AGTTTGATTCTAGCAGAGAT+AGG | 0.388381 | 7.4:+54160490 | MS.gene26056:CDS |
| TCCCACATCGTACTATTATC+AGG | 0.413728 | 7.4:+54161090 | MS.gene26056:CDS |
| CTTCAAGAATAATAATAGAT+AGG | 0.425201 | 7.4:-54160714 | None:intergenic |
| GAAGTCGAAGTCGTCTTTCA+TGG | 0.449885 | 7.4:-54160238 | None:intergenic |
| AAGAAGAAGCTTCTCAAAGA+AGG | 0.467998 | 7.4:+54160327 | MS.gene26056:CDS |
| GTTTGATTCTAGCAGAGATA+GGG | 0.475863 | 7.4:+54160491 | MS.gene26056:CDS |
| TAGGCAGATGGAAGGTGATA+AGG | 0.476180 | 7.4:-54160695 | None:intergenic |
| ATCTATTATTATTCTTGAAG+AGG | 0.490271 | 7.4:+54160717 | MS.gene26056:CDS |
| GACTTCGACTTCGCCGGTGC+CGG | 0.516399 | 7.4:+54160249 | MS.gene26056:CDS |
| AATGAAATTAAAAGCATGGC+AGG | 0.519602 | 7.4:+54161058 | MS.gene26056:CDS |
| AATTGGATTATCGCCGGCAC+CGG | 0.525961 | 7.4:-54160262 | None:intergenic |
| GTGGGTACTAGAGAATTCAT+AGG | 0.534544 | 7.4:+54161116 | MS.gene26056:CDS |
| TTCAATTTCACTCTTGGACA+AGG | 0.538952 | 7.4:+54160522 | MS.gene26056:CDS |
| GTCAAATGAAATTAAAAGCA+TGG | 0.545516 | 7.4:+54161054 | MS.gene26056:CDS |
| AATAATAATAGATAGGCAGA+TGG | 0.568838 | 7.4:-54160707 | None:intergenic |
| CTTGAGAATTGGATTATCGC+CGG | 0.576084 | 7.4:-54160268 | None:intergenic |
| CATATGTAGACACTGTCATA+GGG | 0.583382 | 7.4:-54161147 | None:intergenic |
| TTTGATTCTAGCAGAGATAG+GGG | 0.593989 | 7.4:+54160492 | MS.gene26056:CDS |
| TCGTACTATTATCAGGCAAG+TGG | 0.597519 | 7.4:+54161097 | MS.gene26056:CDS |
| AAAGACGACTTCGACTTCGC+CGG | 0.605504 | 7.4:+54160243 | MS.gene26056:CDS |
| AAGCTTCTCAAAGAAGGTGA+AGG | 0.613170 | 7.4:+54160333 | MS.gene26056:CDS |
| CGGCGATAATCCAATTCTCA+AGG | 0.616014 | 7.4:+54160269 | MS.gene26056:CDS |
| TGCCTGATAATAGTACGATG+TGG | 0.623695 | 7.4:-54161092 | None:intergenic |
| ATAATAGATAGGCAGATGGA+AGG | 0.627317 | 7.4:-54160703 | None:intergenic |
| CGTACTATTATCAGGCAAGT+GGG | 0.636740 | 7.4:+54161098 | MS.gene26056:CDS |
| GCCTGATAATAGTACGATGT+GGG | 0.647015 | 7.4:-54161091 | None:intergenic |
| GATAATCCAATTCTCAAGGT+CGG | 0.682459 | 7.4:+54160273 | MS.gene26056:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGATTTTTGTTATTTTAAT+TGG | + | chr7.4:54160569-54160588 | MS.gene26056:intron | 10.0% |
| !! | AAATCAAACATAGAAATTAA+CGG | - | chr7.4:54160597-54160616 | None:intergenic | 15.0% |
| !!! | TTAATTTCTATGTTTGATTT+AGG | + | chr7.4:54160597-54160616 | MS.gene26056:intron | 15.0% |
| !! | ATCTATTATTATTCTTGAAG+AGG | + | chr7.4:54160717-54160736 | MS.gene26056:CDS | 20.0% |
| !! | CTTCAAGAATAATAATAGAT+AGG | - | chr7.4:54160717-54160736 | None:intergenic | 20.0% |
| !!! | TCTTTTATTTTCTTCACAAA+TGG | - | chr7.4:54160958-54160977 | None:intergenic | 20.0% |
| ! | AATAATAATAGATAGGCAGA+TGG | - | chr7.4:54160710-54160729 | None:intergenic | 25.0% |
| ! | GATTTCAAACAATAAAAAGC+AGG | + | chr7.4:54160806-54160825 | MS.gene26056:intron | 25.0% |
| ! | GTCAAATGAAATTAAAAGCA+TGG | + | chr7.4:54161054-54161073 | MS.gene26056:CDS | 25.0% |
| ! | TGTCATCAATAACATATTGT+TGG | + | chr7.4:54160769-54160788 | MS.gene26056:intron | 25.0% |
| !! | ACCTTTAATTTCCTCATAAT+AGG | - | chr7.4:54160899-54160918 | None:intergenic | 25.0% |
| !! | CCTTTAATTTCCTCATAATA+GGG | - | chr7.4:54160898-54160917 | None:intergenic | 25.0% |
| !! | TGTTTGATTTAGGTTATGTT+TGG | + | chr7.4:54160607-54160626 | MS.gene26056:intron | 25.0% |
| !!! | AGAGGTAAATTTTAAAAGTC+AGG | + | chr7.4:54160735-54160754 | MS.gene26056:intron | 25.0% |
| !!! | CATTTGTTGATAACTACAAA+AGG | - | chr7.4:54160866-54160885 | None:intergenic | 25.0% |
| AATCAACGAAGAAAGAACAT+AGG | - | chr7.4:54160385-54160404 | None:intergenic | 30.0% | |
| ACTACTTTCAATTTCACTCT+TGG | + | chr7.4:54160516-54160535 | MS.gene26056:CDS | 30.0% | |
| CCCTATTATGAGGAAATTAA+AGG | + | chr7.4:54160895-54160914 | MS.gene26056:intron | 30.0% | |
| ! | AATGAAATTAAAAGCATGGC+AGG | + | chr7.4:54161058-54161077 | MS.gene26056:CDS | 30.0% |
| !! | TGAAAAGTGCTTATCATATC+AGG | + | chr7.4:54160661-54160680 | MS.gene26056:intron | 30.0% |
| AAAAAGGAAATCGGAAAGCA+AGG | + | chr7.4:54160300-54160319 | MS.gene26056:CDS | 35.0% | |
| AAGAAGAAGCTTCTCAAAGA+AGG | + | chr7.4:54160327-54160346 | MS.gene26056:CDS | 35.0% | |
| AGTTTGATTCTAGCAGAGAT+AGG | + | chr7.4:54160490-54160509 | MS.gene26056:CDS | 35.0% | |
| ATAATAGATAGGCAGATGGA+AGG | - | chr7.4:54160706-54160725 | None:intergenic | 35.0% | |
| GAGATATGATCCCTATTATG+AGG | + | chr7.4:54160885-54160904 | MS.gene26056:intron | 35.0% | |
| GATAATCCAATTCTCAAGGT+CGG | + | chr7.4:54160273-54160292 | MS.gene26056:CDS | 35.0% | |
| GTCGGAGAAAAAAAGGAAAT+CGG | + | chr7.4:54160291-54160310 | MS.gene26056:CDS | 35.0% | |
| GTTTGATTCTAGCAGAGATA+GGG | + | chr7.4:54160491-54160510 | MS.gene26056:CDS | 35.0% | |
| TATACTAGAACGTTGCTTGA+TGG | + | chr7.4:54160462-54160481 | MS.gene26056:CDS | 35.0% | |
| TTCAATTTCACTCTTGGACA+AGG | + | chr7.4:54160522-54160541 | MS.gene26056:CDS | 35.0% | |
| TTTGATTCTAGCAGAGATAG+GGG | + | chr7.4:54160492-54160511 | MS.gene26056:CDS | 35.0% | |
| !! | GCTGCTTACTTTTGATATTG+TGG | + | chr7.4:54160984-54161003 | MS.gene26056:intron | 35.0% |
| AAGCTTCTCAAAGAAGGTGA+AGG | + | chr7.4:54160333-54160352 | MS.gene26056:CDS | 40.0% | |
| CGTACTATTATCAGGCAAGT+GGG | + | chr7.4:54161098-54161117 | MS.gene26056:CDS | 40.0% | |
| CTTGAGAATTGGATTATCGC+CGG | - | chr7.4:54160271-54160290 | None:intergenic | 40.0% | |
| GCCTGATAATAGTACGATGT+GGG | - | chr7.4:54161094-54161113 | None:intergenic | 40.0% | |
| GTGGGTACTAGAGAATTCAT+AGG | + | chr7.4:54161116-54161135 | MS.gene26056:CDS | 40.0% | |
| TCCCACATCGTACTATTATC+AGG | + | chr7.4:54161090-54161109 | MS.gene26056:CDS | 40.0% | |
| TCGTACTATTATCAGGCAAG+TGG | + | chr7.4:54161097-54161116 | MS.gene26056:CDS | 40.0% | |
| TCTCAAAGAAGGTGAAGGTT+GGG | + | chr7.4:54160338-54160357 | MS.gene26056:intron | 40.0% | |
| TCTCAAGGTCGGAGAAAAAA+AGG | + | chr7.4:54160284-54160303 | MS.gene26056:CDS | 40.0% | |
| TGCCTGATAATAGTACGATG+TGG | - | chr7.4:54161095-54161114 | None:intergenic | 40.0% | |
| TTCTCAAAGAAGGTGAAGGT+TGG | + | chr7.4:54160337-54160356 | MS.gene26056:intron | 40.0% | |
| ! | TTTTCTCCGACCTTGAGAAT+TGG | - | chr7.4:54160282-54160301 | None:intergenic | 40.0% |
| AGGTTAGTGCATTGGAACGA+CGG | + | chr7.4:54160542-54160561 | MS.gene26056:intron | 45.0% | |
| CGGCGATAATCCAATTCTCA+AGG | + | chr7.4:54160269-54160288 | MS.gene26056:CDS | 45.0% | |
| CTTGGACAAGGTTAGTGCAT+TGG | + | chr7.4:54160534-54160553 | MS.gene26056:intron | 45.0% | |
| TAGGCAGATGGAAGGTGATA+AGG | - | chr7.4:54160698-54160717 | None:intergenic | 45.0% | |
| AAAGACGACTTCGACTTCGC+CGG | + | chr7.4:54160243-54160262 | MS.gene26056:CDS | 50.0% | |
| AATTGGATTATCGCCGGCAC+CGG | - | chr7.4:54160265-54160284 | None:intergenic | 50.0% | |
| GGTTGGGATGCTCATGATGT+TGG | + | chr7.4:54160354-54160373 | MS.gene26056:intron | 50.0% | |
| GACTTCGACTTCGCCGGTGC+CGG | + | chr7.4:54160249-54160268 | MS.gene26056:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 54160240 | 54161154 | 54160240 | ID=MS.gene26056 |
| chr7.4 | mRNA | 54160240 | 54161154 | 54160240 | ID=MS.gene26056.t1;Parent=MS.gene26056 |
| chr7.4 | exon | 54160240 | 54160354 | 54160240 | ID=MS.gene26056.t1.exon1;Parent=MS.gene26056.t1 |
| chr7.4 | CDS | 54160240 | 54160354 | 54160240 | ID=cds.MS.gene26056.t1;Parent=MS.gene26056.t1 |
| chr7.4 | exon | 54160457 | 54160543 | 54160457 | ID=MS.gene26056.t1.exon2;Parent=MS.gene26056.t1 |
| chr7.4 | CDS | 54160457 | 54160543 | 54160457 | ID=cds.MS.gene26056.t1;Parent=MS.gene26056.t1 |
| chr7.4 | exon | 54160683 | 54160738 | 54160683 | ID=MS.gene26056.t1.exon3;Parent=MS.gene26056.t1 |
| chr7.4 | CDS | 54160683 | 54160738 | 54160683 | ID=cds.MS.gene26056.t1;Parent=MS.gene26056.t1 |
| chr7.4 | exon | 54161041 | 54161154 | 54161041 | ID=MS.gene26056.t1.exon4;Parent=MS.gene26056.t1 |
| chr7.4 | CDS | 54161041 | 54161154 | 54161041 | ID=cds.MS.gene26056.t1;Parent=MS.gene26056.t1 |
| Gene Sequence |
| Protein sequence |
