AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27119


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTTGTGGGAGCTTATT+TGG 0.141842 1.1:+43724 MS.gene27119:CDS
GCTCTCTCTCTTATTATTCT+TGG 0.167279 1.1:+45230 MS.gene27119:CDS
TCGGTCATGGTTGTGGTTAT+TGG 0.305025 1.1:+44374 MS.gene27119:CDS
ACCAACTTTAAGATGTCTTC+TGG 0.333926 1.1:+45290 MS.gene27119:CDS
CATCCCGGTGATAGATATAT+TGG 0.352609 1.1:-43760 None:intergenic
TTCTAAGCATGTTCCTTTGT+GGG 0.362172 1.1:+43713 MS.gene27119:CDS
CTTCTAAGCATGTTCCTTTG+TGG 0.365168 1.1:+43712 MS.gene27119:CDS
ATCAAAACTAAGCATGATTC+CGG 0.401274 1.1:+44292 MS.gene27119:CDS
TTACCAGCGACTACTTTGAC+TGG 0.414974 1.1:+43630 None:intergenic
ATGGTTGTGGTTATTGGTGT+TGG 0.416143 1.1:+44380 MS.gene27119:CDS
TTTGCTGTAACTGCTCTTGT+CGG 0.454803 1.1:+43660 MS.gene27119:CDS
TTGAGTAGTGTTTGCTGTGA+AGG 0.456859 1.1:-44337 None:intergenic
ATGCTTAGAAGCAGAATAAC+CGG 0.461497 1.1:-43700 None:intergenic
ATAAGAGAGAGAGCTTGAAT+AGG 0.463485 1.1:-45221 None:intergenic
GATGTAAATGTTAACTTCAA+AGG 0.465558 1.1:+44419 MS.gene27119:CDS
CCATTGTTGTGATAGTCAAT+CGG 0.479781 1.1:+45143 MS.gene27119:intron
AGCCTCATGCTCAATTCCGT+TGG 0.485954 1.1:+43789 MS.gene27119:CDS
CTGAGATAGTAATCAACAAA+TGG 0.487131 1.1:-45254 None:intergenic
CGGTGGTTTGTGTGATGGAA+TGG 0.496976 1.1:+44312 MS.gene27119:CDS
ACCAGAAGACATCTTAAAGT+TGG 0.497523 1.1:-45291 None:intergenic
CTACAAAAGAAATATTCGAT+TGG 0.521950 1.1:+45170 MS.gene27119:CDS
GATTCCGGTGGTTTGTGTGA+TGG 0.533242 1.1:+44307 MS.gene27119:CDS
TGATGATACAACTGCTACAC+AGG 0.539546 1.1:-44453 None:intergenic
TTCACAGCAAACACTACTCA+AGG 0.539879 1.1:+44339 MS.gene27119:CDS
GTTGCCAATATATCTATCAC+CGG 0.550759 1.1:+43756 MS.gene27119:CDS
TACCAGCGACTACTTTGACT+GGG 0.553992 1.1:+43631 None:intergenic
GAAAGTGTCGGTCATGGTTG+TGG 0.566114 1.1:+44367 MS.gene27119:CDS
GGAAGTGAAAGTGTCGGTCA+TGG 0.589747 1.1:+44361 MS.gene27119:CDS
GGACTCGTCTCAAATGCTAC+CGG 0.630645 1.1:+43681 MS.gene27119:CDS
CATGAGGCTTAAGTTCATCC+CGG 0.630737 1.1:-43775 None:intergenic
CATTCCATCACACAAACCAC+CGG 0.631892 1.1:-44311 None:intergenic
AAAACTAAGCATGATTCCGG+TGG 0.633913 1.1:+44295 MS.gene27119:CDS
TTTGTTGATTACTATCTCAG+TGG 0.635140 1.1:+45257 MS.gene27119:CDS
TTGCCAATATATCTATCACC+GGG 0.654090 1.1:+43757 MS.gene27119:CDS
GCATACTTGGTAGAATCCAA+CGG 0.658976 1.1:-43805 None:intergenic
TCACAGCAAACACTACTCAA+GGG 0.660525 1.1:+44340 MS.gene27119:CDS
ATCCAACGGAATTGAGCATG+AGG 0.660676 1.1:-43791 None:intergenic
CTCAAGGGAAGTGAAAGTGT+CGG 0.679931 1.1:+44355 MS.gene27119:CDS
AACCAAATAAGCTCCCACAA+AGG 0.703411 1.1:-43726 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAGAAAAAGAAACATT+TGG + chr1.1:44603-44622 MS.gene27119:intron 15.0%
!!! AAAAAAAGAAGTTTGATTTA+AGG - chr1.1:45044-45063 None:intergenic 15.0%
!!! AATAAATCTATTGATTTGTT+GGG - chr1.1:44636-44655 None:intergenic 15.0%
!!! GAAAATGAAAATTTTAAAAC+TGG - chr1.1:44251-44270 None:intergenic 15.0%
!!! TAATAAATCTATTGATTTGT+TGG - chr1.1:44637-44656 None:intergenic 15.0%
!! AAAAAGAAACATTTGGTTAT+GGG + chr1.1:44610-44629 MS.gene27119:intron 20.0%
!! AAAGGACATCTTAATATAAA+TGG - chr1.1:44549-44568 None:intergenic 20.0%
!! AACACTTGAATATAAATAAC+AGG - chr1.1:44575-44594 None:intergenic 20.0%
!! AATATAAATAACAGGAGAAA+AGG - chr1.1:44567-44586 None:intergenic 20.0%
!!! AATGAAAATTTTAAAACTGG+AGG - chr1.1:44248-44267 None:intergenic 20.0%
!!! CATCATTTTTATTCTTTTGA+AGG + chr1.1:44757-44776 MS.gene27119:intron 20.0%
! AAAAAAAATCAGGTATAGCA+AGG - chr1.1:45122-45141 None:intergenic 25.0%
! AAAAAAATCAGGTATAGCAA+GGG - chr1.1:45121-45140 None:intergenic 25.0%
! AATTTAGAAATCAAACTGGT+TGG - chr1.1:45009-45028 None:intergenic 25.0%
! AGATTTAGAGATTAATGAGT+TGG + chr1.1:43988-44007 MS.gene27119:intron 25.0%
! ATGATTCTAAACCAGTAAAT+TGG + chr1.1:44857-44876 MS.gene27119:intron 25.0%
! CTACAAAAGAAATATTCGAT+TGG + chr1.1:45170-45189 MS.gene27119:CDS 25.0%
! GAAAAAGAAACATTTGGTTA+TGG + chr1.1:44609-44628 MS.gene27119:intron 25.0%
! GATGTAAATGTTAACTTCAA+AGG + chr1.1:44419-44438 MS.gene27119:CDS 25.0%
! TGGTAATTTAGAAATCAAAC+TGG - chr1.1:45013-45032 None:intergenic 25.0%
! TGGTCTATGATAGAATATTA+TGG + chr1.1:44023-44042 MS.gene27119:intron 25.0%
!! TTCAGCATCTTTTATTATCT+TGG + chr1.1:44954-44973 MS.gene27119:intron 25.0%
!! TTTGATTTAAGGAAGAAACT+TGG - chr1.1:45033-45052 None:intergenic 25.0%
AAACAGACTTCATTGTTAAC+AGG + chr1.1:44822-44841 MS.gene27119:intron 30.0%
AAACTTCCTGTGTTATATAC+CGG + chr1.1:45070-45089 MS.gene27119:intron 30.0%
AACAGACTTCATTGTTAACA+GGG + chr1.1:44823-44842 MS.gene27119:intron 30.0%
ATCAAAACTAAGCATGATTC+CGG + chr1.1:44292-44311 MS.gene27119:CDS 30.0%
CAACAATGGGAAAAAAAATC+AGG - chr1.1:45132-45151 None:intergenic 30.0%
CAATTACTTGACCAATTTAC+TGG - chr1.1:44871-44890 None:intergenic 30.0%
CAGTAAATTGGTCAAGTAAT+TGG + chr1.1:44869-44888 MS.gene27119:intron 30.0%
CTGAGATAGTAATCAACAAA+TGG - chr1.1:45257-45276 None:intergenic 30.0%
TATCATAGACCACCATATTT+CGG - chr1.1:44015-44034 None:intergenic 30.0%
TCATTACTACAGAATCTACA+GGG - chr1.1:43889-43908 None:intergenic 30.0%
TCTTTACAGCAAATTGTAAG+TGG + chr1.1:44476-44495 MS.gene27119:intron 30.0%
! GATCTAAAATGAACATTGAC+TGG - chr1.1:44802-44821 None:intergenic 30.0%
! TTTGTTGATTACTATCTCAG+TGG + chr1.1:45257-45276 MS.gene27119:CDS 30.0%
ACAGACTTCATTGTTAACAG+GGG + chr1.1:44824-44843 MS.gene27119:intron 35.0%
ACCAGAAGACATCTTAAAGT+TGG - chr1.1:45294-45313 None:intergenic 35.0%
AGATGAAAAGCAAGCATACT+TGG - chr1.1:43821-43840 None:intergenic 35.0%
ATAAGAGAGAGAGCTTGAAT+AGG - chr1.1:45224-45243 None:intergenic 35.0%
ATGCTTAGAAGCAGAATAAC+CGG - chr1.1:43703-43722 None:intergenic 35.0%
CCAACAAATAGCATGATTTC+CGG - chr1.1:45092-45111 None:intergenic 35.0%
CCATTGTTGTGATAGTCAAT+CGG + chr1.1:45143-45162 MS.gene27119:intron 35.0%
CGATTGACTATCACAACAAT+GGG - chr1.1:45145-45164 None:intergenic 35.0%
GCTCTCTCTCTTATTATTCT+TGG + chr1.1:45230-45249 MS.gene27119:CDS 35.0%
GTCATTACTACAGAATCTAC+AGG - chr1.1:43890-43909 None:intergenic 35.0%
GTTGCCAATATATCTATCAC+CGG + chr1.1:43756-43775 MS.gene27119:CDS 35.0%
GTTTCTCTTATAGATTTCCC+AGG - chr1.1:43959-43978 None:intergenic 35.0%
TAAATTCCATATTGCCCACA+TGG - chr1.1:44523-44542 None:intergenic 35.0%
TACAGACACATCTTTCATCT+GGG + chr1.1:44655-44674 MS.gene27119:intron 35.0%
TGATTTCCGGTATATAACAC+AGG - chr1.1:45079-45098 None:intergenic 35.0%
TTACAGACACATCTTTCATC+TGG + chr1.1:44654-44673 MS.gene27119:intron 35.0%
TTCTAAGCATGTTCCTTTGT+GGG + chr1.1:43713-43732 MS.gene27119:CDS 35.0%
TTGAATTGAAGATGAGTGCT+AGG + chr1.1:44701-44720 MS.gene27119:intron 35.0%
TTGCCAATATATCTATCACC+GGG + chr1.1:43757-43776 MS.gene27119:CDS 35.0%
! TAATGAGTTGGACCGAAATA+TGG + chr1.1:44000-44019 MS.gene27119:intron 35.0%
!! ACCAACTTTAAGATGTCTTC+TGG + chr1.1:45290-45309 MS.gene27119:CDS 35.0%
AAAACTAAGCATGATTCCGG+TGG + chr1.1:44295-44314 MS.gene27119:CDS 40.0%
AACCAAATAAGCTCCCACAA+AGG - chr1.1:43729-43748 None:intergenic 40.0%
ATGGTTGTGGTTATTGGTGT+TGG + chr1.1:44380-44399 MS.gene27119:CDS 40.0%
CATCCCGGTGATAGATATAT+TGG - chr1.1:43763-43782 None:intergenic 40.0%
CCGATTGACTATCACAACAA+TGG - chr1.1:45146-45165 None:intergenic 40.0%
CTTCTAAGCATGTTCCTTTG+TGG + chr1.1:43712-43731 MS.gene27119:CDS 40.0%
GAAAGTGAAAACACGAGAGT+AGG - chr1.1:44214-44233 None:intergenic 40.0%
GCATACTTGGTAGAATCCAA+CGG - chr1.1:43808-43827 None:intergenic 40.0%
TCAATCCTTAACCCACTAAC+CGG - chr1.1:44074-44093 None:intergenic 40.0%
TCACAGCAAACACTACTCAA+GGG + chr1.1:44340-44359 MS.gene27119:CDS 40.0%
TGATGATACAACTGCTACAC+AGG - chr1.1:44456-44475 None:intergenic 40.0%
TTCACAGCAAACACTACTCA+AGG + chr1.1:44339-44358 MS.gene27119:CDS 40.0%
TTCCTTTGTGGGAGCTTATT+TGG + chr1.1:43724-43743 MS.gene27119:CDS 40.0%
! CCGGAAATCATGCTATTTGT+TGG + chr1.1:45089-45108 MS.gene27119:intron 40.0%
! TTTGCTGTAACTGCTCTTGT+CGG + chr1.1:43660-43679 MS.gene27119:CDS 40.0%
!! CTTCCTTTTAGAGACATGTG+CGG + chr1.1:44141-44160 MS.gene27119:intron 40.0%
!! TTGAGTAGTGTTTGCTGTGA+AGG - chr1.1:44340-44359 None:intergenic 40.0%
AATGTGTCTGTTCCACCATG+TGG + chr1.1:44505-44524 MS.gene27119:intron 45.0%
ATCCAACGGAATTGAGCATG+AGG - chr1.1:43794-43813 None:intergenic 45.0%
ATGTGTCTGTTCCACCATGT+GGG + chr1.1:44506-44525 MS.gene27119:intron 45.0%
ATTCCATATTGCCCACATGG+TGG - chr1.1:44520-44539 None:intergenic 45.0%
CATGAGGCTTAAGTTCATCC+CGG - chr1.1:43778-43797 None:intergenic 45.0%
CATTCCATCACACAAACCAC+CGG - chr1.1:44314-44333 None:intergenic 45.0%
CTACCGCACATGTCTCTAAA+AGG - chr1.1:44147-44166 None:intergenic 45.0%
TCGGTCATGGTTGTGGTTAT+TGG + chr1.1:44374-44393 MS.gene27119:CDS 45.0%
TGTTCATCAGCACTATTCGC+TGG - chr1.1:44902-44921 None:intergenic 45.0%
! AGATTTCCCAGGTTTTCCAC+TGG - chr1.1:43948-43967 None:intergenic 45.0%
! CTCAAGGGAAGTGAAAGTGT+CGG + chr1.1:44355-44374 MS.gene27119:CDS 45.0%
! TGAGTTGGACCGAAATATGG+TGG + chr1.1:44003-44022 MS.gene27119:intron 45.0%
ACTACAGCCAGTGGAAAACC+TGG + chr1.1:43938-43957 MS.gene27119:intron 50.0%
AGCCTCATGCTCAATTCCGT+TGG + chr1.1:43789-43808 MS.gene27119:CDS 50.0%
AGTCAGATCACTACAGCCAG+TGG + chr1.1:43929-43948 MS.gene27119:intron 50.0%
CTACAGCCAGTGGAAAACCT+GGG + chr1.1:43939-43958 MS.gene27119:intron 50.0%
CTTAACCCACTAACCGGAGT+CGG - chr1.1:44068-44087 None:intergenic 50.0%
GGACTCGTCTCAAATGCTAC+CGG + chr1.1:43681-43700 MS.gene27119:CDS 50.0%
GTTCCACCATGTGGGCAATA+TGG + chr1.1:44514-44533 MS.gene27119:intron 50.0%
! CGGTGGTTTGTGTGATGGAA+TGG + chr1.1:44312-44331 MS.gene27119:CDS 50.0%
! GATTCCGGTGGTTTGTGTGA+TGG + chr1.1:44307-44326 MS.gene27119:CDS 50.0%
! TTGATCCATGTAGCCGACTC+CGG + chr1.1:44052-44071 MS.gene27119:intron 50.0%
!! GAAAGTGTCGGTCATGGTTG+TGG + chr1.1:44367-44386 MS.gene27119:CDS 50.0%
!! GGAAGTGAAAGTGTCGGTCA+TGG + chr1.1:44361-44380 MS.gene27119:CDS 50.0%
ACTAACCGGAGTCGGCTACA+TGG - chr1.1:44060-44079 None:intergenic 55.0%
! ATGTAGCCGACTCCGGTTAG+TGG + chr1.1:44059-44078 MS.gene27119:intron 55.0%
! TGTAGCCGACTCCGGTTAGT+GGG + chr1.1:44060-44079 MS.gene27119:intron 55.0%
!! CGACTCCGGTTAGTGGGTTA+AGG + chr1.1:44066-44085 MS.gene27119:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 43637 45319 43637 ID=MS.gene27119
chr1.1 mRNA 43637 45319 43637 ID=MS.gene27119.t1;Parent=MS.gene27119
chr1.1 exon 43637 43821 43637 ID=MS.gene27119.t1.exon1;Parent=MS.gene27119.t1
chr1.1 CDS 43637 43821 43637 ID=cds.MS.gene27119.t1;Parent=MS.gene27119.t1
chr1.1 exon 44290 44490 44290 ID=MS.gene27119.t1.exon2;Parent=MS.gene27119.t1
chr1.1 CDS 44290 44490 44290 ID=cds.MS.gene27119.t1;Parent=MS.gene27119.t1
chr1.1 exon 45158 45319 45158 ID=MS.gene27119.t1.exon3;Parent=MS.gene27119.t1
chr1.1 CDS 45158 45319 45158 ID=cds.MS.gene27119.t1;Parent=MS.gene27119.t1
Gene Sequence

>MS.gene27119

CGACTACTTTGACTGGGTTTCACTTTGCTGTAACTGCTCTTGTCGGACTCGTCTCAAATGCTACCGGTTATTCTGCTTCTAAGCATGTTCCTTTGTGGGAGCTTATTTGGTTCTCTGCTGTTGCCAATATATCTATCACCGGGATGAACTTAAGCCTCATGCTCAATTCCGTTGGATTCTACCAAGTATGCTTGCTTTTCATCTTTACTCTTTCAACTTCATTCAATTTTCAAGATGTTAGAGAGAAAACCCTGTAGATTCTGTAGTAATGACAGTAGATCAGATGAAACATAGTCAGATCACTACAGCCAGTGGAAAACCTGGGAAATCTATAAGAGAAACTATTAAAAAAGATTTAGAGATTAATGAGTTGGACCGAAATATGGTGGTCTATGATAGAATATTATGGCATAATTTGATCCATGTAGCCGACTCCGGTTAGTGGGTTAAGGATTGATTGATTGATTGTCTGCATCTTGTAATAATTTCAAATTATGCTCAACTCTTCCTTTTAGAGACATGTGCGGTAGTTATTATAATTACATCTCATGTGCTCTAACTTTTAAATGAGCAGCCTACTCTCGTGTTTTCACTTTCAATCTTCATTTCCTCCAGTTTTAAAATTTTCATTTTCTCATCGTGTATGTGAACAGATATCAAAACTAAGCATGATTCCGGTGGTTTGTGTGATGGAATGGATCCTTCACAGCAAACACTACTCAAGGGAAGTGAAAGTGTCGGTCATGGTTGTGGTTATTGGTGTTGGTGTTTGCACTGTAACTGATGTAAATGTTAACTTCAAAGGTTTTGTGTGCGCCTGTGTAGCAGTTGTATCATCATCTTTACAGCAAATTGTAAGTGGTATATAAATGTGTCTGTTCCACCATGTGGGCAATATGGAATTTATCTCCATTTATATTAAGATGTCCTTTTCTCCTGTTATTTATATTCAAGTGTTGTACTACGATAATAGAAAAAGAAACATTTGGTTATGGGCCCAACAAATCAATAGATTTATTACAGACACATCTTTCATCTGGGATTTTTTAATTCTTTTGTACATTTTGAATTGAAGATGAGTGCTAGGTGAAACTTGCATAGACGTAGTATCATGAGTGAGCATCATTTTTATTCTTTTGAAGGTTTTTTAACTTGTACTTTGCCAGTCAATGTTCATTTTAGATCAAACAGACTTCATTGTTAACAGGGGAGTAGAAAACATGATTCTAAACCAGTAAATTGGTCAAGTAATTGGTTCAAAACCAGCGAATAGTGCTGATGAACACTGTTGTTTTTCATTTTTTTTATCTTTTTTTTTTCAGCATCTTTTATTATCTTGGTAATCATTTTAATTTGAATTTCATAATAACCAACCAGTTTGATTTCTAAATTACCAAGTTTCTTCCTTAAATCAAACTTCTTTTTTTTACAAGAAACTTCCTGTGTTATATACCGGAAATCATGCTATTTGTTGGATTGATCCCTTGCTATACCTGATTTTTTTTCCCATTGTTGTGATAGTCAATCGGTTCTCTACAAAAGAAATATTCGATTGGATCTTTTGAACTGCTGAGTAAGACTGCTCCTATTCAAGCTCTCTCTCTTATTATTCTTGGTCCATTTGTTGATTACTATCTCAGTGGCAAGTTGATAACCAACTTTAAGATGTCTTCTGGTGCCATT

Protein sequence

>MS.gene27119.t1

RLLULGFTLLULLLSDSSQMLPVILLLSMFLCGSLFGSLLLPIYLSPGUTUASCSIPLDSTKYQNUAUFRWFVUWNGSFTANTTQGKUKCRSWLWLLVLVFALULMUMLTSKVLCAPVUQLYHHLYSKFQSVLYKRNIRLDLLNCUVRLLLFKLSLLLFLVHLLITISVASUUPTLRCLLVP