AlfalfaGEDB | MS.gene28196

M. sativa cultivar XinJiangDaYe / MS.gene28196

KEGG: -
GO: -
PFAM: PF06799.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28196.t1	KEH39935.1	98.1	154	3	0	1	154	1	154	2.00E-76	295

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28196.t1	Q9FN15	33.1	127	84	1	27	153	58	183	1.6e-10	67.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28196.t1	A0A072VP92	98.1	154	3	0	1	154	1	154	1.4e-76	295.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051228	MS.gene28196	0.814378	1.59E-51	-1.69E-46
MS.gene051617	MS.gene28196	0.86296	3.50E-64	-1.69E-46
MS.gene051666	MS.gene28196	0.809558	1.78E-50	-1.69E-46
MS.gene051692	MS.gene28196	0.808163	3.54E-50	-1.69E-46
MS.gene051810	MS.gene28196	0.813829	2.10E-51	-1.69E-46
MS.gene051838	MS.gene28196	0.81187	5.64E-51	-1.69E-46
MS.gene053207	MS.gene28196	0.817883	2.62E-52	-1.69E-46
MS.gene053632	MS.gene28196	0.812204	4.77E-51	-1.69E-46
MS.gene053824	MS.gene28196	0.809	2.35E-50	-1.69E-46
MS.gene053916	MS.gene28196	0.819482	1.13E-52	-1.69E-46
MS.gene054600	MS.gene28196	0.817478	3.23E-52	-1.69E-46
MS.gene054642	MS.gene28196	0.801374	9.28E-49	-1.69E-46
MS.gene05494	MS.gene28196	0.803294	3.73E-49	-1.69E-46
MS.gene055224	MS.gene28196	0.800375	1.48E-48	-1.69E-46
MS.gene055577	MS.gene28196	0.832789	7.74E-56	-1.69E-46
MS.gene05592	MS.gene28196	0.805497	1.30E-49	-1.69E-46
MS.gene055941	MS.gene28196	0.809001	2.35E-50	-1.69E-46
MS.gene056487	MS.gene28196	0.800655	1.30E-48	-1.69E-46
MS.gene05666	MS.gene28196	0.805095	1.57E-49	-1.69E-46
MS.gene056897	MS.gene28196	0.812416	4.29E-51	-1.69E-46
MS.gene057124	MS.gene28196	0.810612	1.06E-50	-1.69E-46
MS.gene057129	MS.gene28196	0.804466	2.13E-49	-1.69E-46
MS.gene057926	MS.gene28196	0.843696	1.20E-58	-1.69E-46
MS.gene058006	MS.gene28196	0.818766	1.65E-52	-1.69E-46
MS.gene058723	MS.gene28196	0.818457	1.94E-52	-1.69E-46
MS.gene058838	MS.gene28196	0.815733	7.95E-52	-1.69E-46
MS.gene060193	MS.gene28196	0.810407	1.17E-50	-1.69E-46
MS.gene060762	MS.gene28196	0.80678	6.97E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28196.t1	MTR_1g016360	98.052	154	3	0	1	154	1	154	2.29e-108	306
MS.gene28196.t1	MTR_1g016360	98.052	154	3	0	1	154	1	154	1.13e-107	305
MS.gene28196.t1	MTR_1g016360	98.052	154	3	0	1	154	1	154	6.50e-107	305
MS.gene28196.t1	MTR_5g016100	36.538	104	65	1	50	153	66	168	2.86e-15	71.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28196.t1	AT5G11840	58.442	154	52	2	13	154	9	162	1.84e-57	178
MS.gene28196.t1	AT5G11840	58.442	154	52	2	13	154	9	162	2.00e-57	178
MS.gene28196.t1	AT5G11840	58.442	154	52	2	13	154	9	162	4.42e-57	177
MS.gene28196.t1	AT5G11840	58.442	154	52	2	13	154	9	162	1.10e-56	178
MS.gene28196.t1	AT5G67370	32.353	136	91	1	18	153	49	183	2.48e-15	72.0

Find 37 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACGACTGGTACTCGTTTA+TGG	0.126473	1.2:+6177511	None:intergenic
GTTGATGGAGGGAATAGTTT+TGG	0.223696	1.2:+6177665	None:intergenic
TGCTGCTTCTAGTGGTGTTT+TGG	0.278118	1.2:-6177330	MS.gene28196:CDS
TGTTGGTGTGAATGGGAAAA+AGG	0.279842	1.2:+6177636	None:intergenic
CTTTGTTCTGTTGCTGTTGT+TGG	0.287030	1.2:+6177598	None:intergenic
TGGCTTGGTTTGGAACTGTT+GGG	0.311583	1.2:-6177385	MS.gene28196:CDS
GCTGGTAATGTTGTTGAATA+TGG	0.316599	1.2:-6177461	MS.gene28196:CDS
TTACCAGCAGCCCATGAAAA+TGG	0.328778	1.2:+6177476	None:intergenic
GGTAATGGTGATTGATTTGT+TGG	0.349076	1.2:+6177619	None:intergenic
GGGTTACCTGTGGCTTGGTT+TGG	0.356647	1.2:-6177395	MS.gene28196:CDS
AACGACTGGTACTCGTTTAT+GGG	0.360388	1.2:+6177512	None:intergenic
TCTGTTGCTGTTGTTGGTAA+TGG	0.366769	1.2:+6177604	None:intergenic
GTGGCTTGGTTTGGAACTGT+TGG	0.374589	1.2:-6177386	MS.gene28196:CDS
TTGTTAGGATGTATCTTGGT+TGG	0.396546	1.2:-6177292	MS.gene28196:CDS
GATTGATTTGTTGGTGTGAA+TGG	0.406949	1.2:+6177628	None:intergenic
TGTTAGGATGTATCTTGGTT+GGG	0.425759	1.2:-6177291	MS.gene28196:CDS
GCGGTTGTTAGGATGTATCT+TGG	0.430246	1.2:-6177296	MS.gene28196:CDS
AAAAGCCCACAACGAAAAGC+CGG	0.436094	1.2:+6177435	None:intergenic
ATGTTGTTGAATATGGTTCC+CGG	0.438372	1.2:-6177454	MS.gene28196:CDS
GGCTGCTGTTCTAACGGAAC+AGG	0.440485	1.2:+6177533	None:intergenic
ATCTTGGTTGGGCTTATGTT+GGG	0.441526	1.2:-6177280	MS.gene28196:CDS
CTCTGTTTAAGTTCAAGAAA+CGG	0.462020	1.2:-6177566	MS.gene28196:CDS
TATCTTGGTTGGGCTTATGT+TGG	0.471423	1.2:-6177281	MS.gene28196:CDS
TTGTGGGGTTACCTGTGGCT+TGG	0.484321	1.2:-6177400	MS.gene28196:CDS
ATTGATTTGTTGGTGTGAAT+GGG	0.490693	1.2:+6177629	None:intergenic
TTTCCTTTGCCTTGCTTGTG+GGG	0.493230	1.2:-6177415	MS.gene28196:CDS
GGCTTGGTTTGGAACTGTTG+GGG	0.506638	1.2:-6177384	MS.gene28196:CDS
GCACAAACAATTCTCTTAGC+AGG	0.508243	1.2:+6177350	None:intergenic
ATTGTTTGTGCTGCTTCTAG+TGG	0.521546	1.2:-6177338	MS.gene28196:CDS
AAAGCCCACAACGAAAAGCC+GGG	0.532515	1.2:+6177436	None:intergenic
TTTATGGGCTGCTGTTCTAA+CGG	0.540735	1.2:+6177527	None:intergenic
TAACCCCACAAGCAAGGCAA+AGG	0.556717	1.2:+6177412	None:intergenic
CTTGCTTGTGGGGTTACCTG+TGG	0.557353	1.2:-6177405	MS.gene28196:CDS
CACAGGTAACCCCACAAGCA+AGG	0.573824	1.2:+6177406	None:intergenic
GAAACGATGTCGATAACGAC+TGG	0.590284	1.2:+6177498	None:intergenic
ACAGTTCCAAACCAAGCCAC+AGG	0.624924	1.2:+6177389	None:intergenic
AGATACATTACATTCAACAG+TGG	0.729086	1.2:+6177241	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTCTGTTTAAGTTCAAGAAA+CGG	-	chr1.2:6177373-6177392	MS.gene28196:CDS	30.0%
!!	ATTGATTTGTTGGTGTGAAT+GGG	+	chr1.2:6177313-6177332	None:intergenic	30.0%
	ATGTTGTTGAATATGGTTCC+CGG	-	chr1.2:6177485-6177504	MS.gene28196:CDS	35.0%
	TGTTAGGATGTATCTTGGTT+GGG	-	chr1.2:6177648-6177667	MS.gene28196:CDS	35.0%
	TTGTTAGGATGTATCTTGGT+TGG	-	chr1.2:6177647-6177666	MS.gene28196:CDS	35.0%
!	GACATCGTTTCCATTTTCAT+GGG	-	chr1.2:6177453-6177472	MS.gene28196:CDS	35.0%
!	GCTGGTAATGTTGTTGAATA+TGG	-	chr1.2:6177478-6177497	MS.gene28196:CDS	35.0%
!	GGAAAAAGGGTTTTTGTTGA+TGG	+	chr1.2:6177292-6177311	None:intergenic	35.0%
!	TTGATGGAGGGAATAGTTTT+GGG	+	chr1.2:6177276-6177295	None:intergenic	35.0%
!!	AAAAGGGTTTTTGTTGATGG+AGG	+	chr1.2:6177289-6177308	None:intergenic	35.0%
!!	AAAGGGTTTTTGTTGATGGA+GGG	+	chr1.2:6177288-6177307	None:intergenic	35.0%
!!	GATTGATTTGTTGGTGTGAA+TGG	+	chr1.2:6177314-6177333	None:intergenic	35.0%
!!	GGTAATGGTGATTGATTTGT+TGG	+	chr1.2:6177323-6177342	None:intergenic	35.0%
!!!	TGTTTTGGCTGTTACTTTTG+CGG	-	chr1.2:6177624-6177643	MS.gene28196:CDS	35.0%
	AACGACTGGTACTCGTTTAT+GGG	+	chr1.2:6177430-6177449	None:intergenic	40.0%
	ATCTTGGTTGGGCTTATGTT+GGG	-	chr1.2:6177659-6177678	MS.gene28196:CDS	40.0%
	GCACAAACAATTCTCTTAGC+AGG	+	chr1.2:6177592-6177611	None:intergenic	40.0%
	GTTGGTGTGAATGGGAAAAA+GGG	+	chr1.2:6177305-6177324	None:intergenic	40.0%
	TAACGACTGGTACTCGTTTA+TGG	+	chr1.2:6177431-6177450	None:intergenic	40.0%
	TATCTTGGTTGGGCTTATGT+TGG	-	chr1.2:6177658-6177677	MS.gene28196:CDS	40.0%
	TGTTGGTGTGAATGGGAAAA+AGG	+	chr1.2:6177306-6177325	None:intergenic	40.0%
!	CGACATCGTTTCCATTTTCA+TGG	-	chr1.2:6177452-6177471	MS.gene28196:CDS	40.0%
!	CTTTGTTCTGTTGCTGTTGT+TGG	+	chr1.2:6177344-6177363	None:intergenic	40.0%
!	TCTGTTGCTGTTGTTGGTAA+TGG	+	chr1.2:6177338-6177357	None:intergenic	40.0%
!	TTTATGGGCTGCTGTTCTAA+CGG	+	chr1.2:6177415-6177434	None:intergenic	40.0%
!	TTTTCCTTTGCCTTGCTTGT+GGG	-	chr1.2:6177523-6177542	MS.gene28196:CDS	40.0%
!!	ATTGTTTGTGCTGCTTCTAG+TGG	-	chr1.2:6177601-6177620	MS.gene28196:CDS	40.0%
!!	GTTGATGGAGGGAATAGTTT+TGG	+	chr1.2:6177277-6177296	None:intergenic	40.0%
!!	TGATGGAGGGAATAGTTTTG+GGG	+	chr1.2:6177275-6177294	None:intergenic	40.0%
!!!	CTGTTACTTTTGCGGTTGTT+AGG	-	chr1.2:6177632-6177651	MS.gene28196:CDS	40.0%
	AAAAGCCCACAACGAAAAGC+CGG	+	chr1.2:6177507-6177526	None:intergenic	45.0%
	GAAACGATGTCGATAACGAC+TGG	+	chr1.2:6177444-6177463	None:intergenic	45.0%
	GCGGTTGTTAGGATGTATCT+TGG	-	chr1.2:6177643-6177662	MS.gene28196:CDS	45.0%
	TTACCAGCAGCCCATGAAAA+TGG	+	chr1.2:6177466-6177485	None:intergenic	45.0%
	TTTCCTTTGCCTTGCTTGTG+GGG	-	chr1.2:6177524-6177543	MS.gene28196:CDS	45.0%
!	CTTTTCCTTTGCCTTGCTTG+TGG	-	chr1.2:6177522-6177541	MS.gene28196:CDS	45.0%
!!	TGCTGCTTCTAGTGGTGTTT+TGG	-	chr1.2:6177609-6177628	MS.gene28196:CDS	45.0%
!!	TGGCTTGGTTTGGAACTGTT+GGG	-	chr1.2:6177554-6177573	MS.gene28196:CDS	45.0%
!!	TTTCCATTTTCATGGGCTGC+TGG	-	chr1.2:6177460-6177479	MS.gene28196:CDS	45.0%
!!!	TAGTTTTGGGGACATGCGTA+TGG	+	chr1.2:6177263-6177282	None:intergenic	45.0%
	AAAGCCCACAACGAAAAGCC+GGG	+	chr1.2:6177506-6177525	None:intergenic	50.0%
	ACAGTTCCAAACCAAGCCAC+AGG	+	chr1.2:6177553-6177572	None:intergenic	50.0%
	TAACCCCACAAGCAAGGCAA+AGG	+	chr1.2:6177530-6177549	None:intergenic	50.0%
!!	GGCTTGGTTTGGAACTGTTG+GGG	-	chr1.2:6177555-6177574	MS.gene28196:CDS	50.0%
!!	GTGGCTTGGTTTGGAACTGT+TGG	-	chr1.2:6177553-6177572	MS.gene28196:CDS	50.0%
	CACAGGTAACCCCACAAGCA+AGG	+	chr1.2:6177536-6177555	None:intergenic	55.0%
	CTTGCTTGTGGGGTTACCTG+TGG	-	chr1.2:6177534-6177553	MS.gene28196:CDS	55.0%
	GGCTGCTGTTCTAACGGAAC+AGG	+	chr1.2:6177409-6177428	None:intergenic	55.0%
	TTGTGGGGTTACCTGTGGCT+TGG	-	chr1.2:6177539-6177558	MS.gene28196:CDS	55.0%
!	GGTTCCCGGCTTTTCGTTGT+GGG	-	chr1.2:6177499-6177518	MS.gene28196:CDS	55.0%
!	TGGTTCCCGGCTTTTCGTTG+TGG	-	chr1.2:6177498-6177517	MS.gene28196:CDS	55.0%
!!	GGGTTACCTGTGGCTTGGTT+TGG	-	chr1.2:6177544-6177563	MS.gene28196:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	6177247	6177714	6177247	ID=MS.gene28196
chr1.2	mRNA	6177247	6177714	6177247	ID=MS.gene28196.t1;Parent=MS.gene28196
chr1.2	exon	6177247	6177714	6177247	ID=MS.gene28196.t1.exon1;Parent=MS.gene28196.t1
chr1.2	CDS	6177247	6177714	6177247	ID=cds.MS.gene28196.t1;Parent=MS.gene28196.t1

Gene Sequence

>MS.gene28196

ATGGCAACAATATCCATACGCATGTCCCCAAAACTATTCCCTCCATCAACAAAAACCCTTTTTCCCATTCACACCAACAAATCAATCACCATTACCAACAACAGCAACAGAACAAAGCTATCGTTGCTCTGTTTAAGTTCAAGAAACGGAACAGAGTGTCCTGTTCCGTTAGAACAGCAGCCCATAAACGAGTACCAGTCGTTATCGACATCGTTTCCATTTTCATGGGCTGCTGGTAATGTTGTTGAATATGGTTCCCGGCTTTTCGTTGTGGGCTTTTCCTTTGCCTTGCTTGTGGGGTTACCTGTGGCTTGGTTTGGAACTGTTGGGGCACAATATGAGCCTGCTAAGAGAATTGTTTGTGCTGCTTCTAGTGGTGTTTTGGCTGTTACTTTTGCGGTTGTTAGGATGTATCTTGGTTGGGCTTATGTTGGGAATCGTTTGCTCAGTGCCACTGTTGAATGTAAT

Protein sequence

>MS.gene28196.t1

MATISIRMSPKLFPPSTKTLFPIHTNKSITITNNSNRTKLSLLCLSSRNGTECPVPLEQQPINEYQSLSTSFPFSWAAGNVVEYGSRLFVVGFSFALLVGLPVAWFGTVGAQYEPAKRIVCAASSGVLAVTFAVVRMYLGWAYVGNRLLSATVECN