Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32183.t1 | XP_003608189.2 | 98.9 | 93 | 1 | 0 | 64 | 156 | 63 | 155 | 2.50E-26 | 128.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32183.t1 | G7JQS4 | 98.9 | 93 | 1 | 0 | 64 | 156 | 63 | 155 | 1.8e-26 | 128.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene32183 | 0.809297 | 2.03E-50 | -1.69E-46 |
| MS.gene049350 | MS.gene32183 | 0.814384 | 1.58E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene32183 | 0.805796 | 1.12E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene32183 | 0.805513 | 1.29E-49 | -1.69E-46 |
| MS.gene050437 | MS.gene32183 | 0.847523 | 1.10E-59 | -1.69E-46 |
| MS.gene050689 | MS.gene32183 | 0.812799 | 3.53E-51 | -1.69E-46 |
| MS.gene050752 | MS.gene32183 | 0.851667 | 7.64E-61 | -1.69E-46 |
| MS.gene050955 | MS.gene32183 | 0.830647 | 2.61E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene32183 | 0.826622 | 2.45E-54 | -1.69E-46 |
| MS.gene051234 | MS.gene32183 | 0.820325 | 7.28E-53 | -1.69E-46 |
| MS.gene051343 | MS.gene32183 | 0.812918 | 3.33E-51 | -1.69E-46 |
| MS.gene05134 | MS.gene32183 | 0.800375 | 1.48E-48 | -1.69E-46 |
| MS.gene052072 | MS.gene32183 | 0.823481 | 1.35E-53 | -1.69E-46 |
| MS.gene052133 | MS.gene32183 | 0.819899 | 9.11E-53 | -1.69E-46 |
| MS.gene052516 | MS.gene32183 | 0.80839 | 3.17E-50 | -1.69E-46 |
| MS.gene052947 | MS.gene32183 | 0.803405 | 3.54E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene32183 | 0.803603 | 3.22E-49 | -1.69E-46 |
| MS.gene05364 | MS.gene32183 | 0.865384 | 6.14E-65 | -1.69E-46 |
| MS.gene054403 | MS.gene32183 | 0.849529 | 3.05E-60 | -1.69E-46 |
| MS.gene056005 | MS.gene32183 | 0.802941 | 4.42E-49 | -1.69E-46 |
| MS.gene057694 | MS.gene32183 | 0.839471 | 1.55E-57 | -1.69E-46 |
| MS.gene057705 | MS.gene32183 | 0.824744 | 6.84E-54 | -1.69E-46 |
| MS.gene058852 | MS.gene32183 | 0.804944 | 1.69E-49 | -1.69E-46 |
| MS.gene059227 | MS.gene32183 | 0.811898 | 5.56E-51 | -1.69E-46 |
| MS.gene060244 | MS.gene32183 | 0.824687 | 7.05E-54 | -1.69E-46 |
| MS.gene061100 | MS.gene32183 | 0.822389 | 2.43E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32183.t1 | MTR_4g090520 | 98.077 | 156 | 2 | 1 | 1 | 156 | 1 | 155 | 3.18e-102 | 290 |
| MS.gene32183.t1 | MTR_2g012520 | 87.097 | 62 | 8 | 0 | 95 | 156 | 98 | 159 | 1.01e-26 | 98.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32183.t1 | AT4G21620 | 62.963 | 108 | 36 | 3 | 49 | 156 | 28 | 131 | 7.69e-18 | 75.1 |
| MS.gene32183.t1 | AT4G21620 | 78.947 | 57 | 12 | 0 | 100 | 156 | 42 | 98 | 2.05e-17 | 73.2 |
| MS.gene32183.t1 | AT1G61255 | 47.154 | 123 | 47 | 4 | 36 | 156 | 37 | 143 | 8.07e-16 | 70.1 |
Find 61 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAATTATGCAGGTGGATA+TGG | 0.249805 | 4.4:+22974556 | MS.gene32183:CDS |
| GGTGGATATGGAGGTGGATA+TGG | 0.281988 | 4.4:+22974568 | MS.gene32183:CDS |
| TATGGTGGCTCCTTTGGATT+TGG | 0.284103 | 4.4:+22974478 | MS.gene32183:CDS |
| CTGGACAGGTGACCTTCTTC+TGG | 0.290223 | 4.4:-22974666 | None:intergenic |
| GGCTACGGTGGTGGCGGTGG+AGG | 0.319831 | 4.4:+22974724 | MS.gene32183:CDS |
| AATGACTATGGTGGCTCCTT+TGG | 0.327553 | 4.4:+22974472 | MS.gene32183:CDS |
| TACGGTGGTGGCGGTGGAGG+TGG | 0.331710 | 4.4:+22974727 | MS.gene32183:CDS |
| GTTGGTGGTGGCAATGACTA+TGG | 0.362645 | 4.4:+22974460 | MS.gene32183:CDS |
| AAGGGCTACGGTGGTGGCGG+TGG | 0.371027 | 4.4:+22974721 | MS.gene32183:CDS |
| GCAAGATGGTGAGCAAAATA+AGG | 0.379174 | 4.4:-22974342 | None:intergenic |
| TTTGTAGAGTGCCCTCCATT+TGG | 0.386250 | 4.4:-22974595 | None:intergenic |
| GGTGGTGGCGGTGGAGGTGG+TGG | 0.408190 | 4.4:+22974730 | MS.gene32183:CDS |
| GTCAGCAGATGGTGAAGGTT+TGG | 0.409569 | 4.4:-22974410 | None:intergenic |
| GCCACCACCGTAGCCCTTGC+CGG | 0.412928 | 4.4:-22974716 | None:intergenic |
| TCCGGCAAGGGCTACGGTGG+TGG | 0.414517 | 4.4:+22974715 | MS.gene32183:CDS |
| AAGGAAGTAAAACACCTTGC+TGG | 0.414681 | 4.4:-22974685 | None:intergenic |
| GGCTCCTTTGGATTTGGACC+AGG | 0.449384 | 4.4:+22974484 | MS.gene32183:CDS |
| GGTGGATATGGTGGTCCAAA+TGG | 0.453271 | 4.4:+22974580 | MS.gene32183:CDS |
| TTCCTTCAGTCGCTCCGGCA+AGG | 0.458448 | 4.4:+22974702 | MS.gene32183:CDS |
| TTTGGATTTGGACCAGGAGG+AGG | 0.459507 | 4.4:+22974490 | MS.gene32183:CDS |
| AGTAAAACACCTTGCTGGAC+AGG | 0.461202 | 4.4:-22974680 | None:intergenic |
| TCCTTCAGTCGCTCCGGCAA+GGG | 0.470860 | 4.4:+22974703 | MS.gene32183:CDS |
| AATGTACTGCTTACTGTTGA+GGG | 0.477371 | 4.4:+22974776 | MS.gene32183:CDS |
| CTGTCTTTGCAGACAACAGT+TGG | 0.480004 | 4.4:-22974631 | None:intergenic |
| AGTCGCTCCGGCAAGGGCTA+CGG | 0.483160 | 4.4:+22974709 | MS.gene32183:CDS |
| GGCAAGGGCTACGGTGGTGG+CGG | 0.485399 | 4.4:+22974718 | MS.gene32183:CDS |
| TGAAGGTTTGGTTGTTGATG+TGG | 0.499325 | 4.4:-22974398 | None:intergenic |
| TCCTTTGGATTTGGACCAGG+AGG | 0.509727 | 4.4:+22974487 | MS.gene32183:CDS |
| AAATGTACTGCTTACTGTTG+AGG | 0.516007 | 4.4:+22974775 | MS.gene32183:CDS |
| GTGACCTTCTTCTGGTAACA+AGG | 0.517811 | 4.4:-22974658 | None:intergenic |
| AATGGAGGGCACTCTACAAA+TGG | 0.522558 | 4.4:+22974598 | MS.gene32183:CDS |
| AACAACAACAATAACAAAGA+TGG | 0.524444 | 4.4:+22974436 | MS.gene32183:CDS |
| TTGTTGTTGTTGTCAGCAGA+TGG | 0.534850 | 4.4:-22974421 | None:intergenic |
| GGATATGGTGGTCCAAATGG+AGG | 0.547027 | 4.4:+22974583 | MS.gene32183:CDS |
| TTTACTTCCTTCAGTCGCTC+CGG | 0.547199 | 4.4:+22974697 | MS.gene32183:CDS |
| GCCCTTGCCGGAGCGACTGA+AGG | 0.553888 | 4.4:-22974704 | None:intergenic |
| GGAGGAGGAAATTATGCAGG+TGG | 0.555457 | 4.4:+22974550 | MS.gene32183:CDS |
| CGCTCCGGCAAGGGCTACGG+TGG | 0.556652 | 4.4:+22974712 | MS.gene32183:CDS |
| AACAAAGATGGTGTTGGTGG+TGG | 0.557994 | 4.4:+22974448 | MS.gene32183:CDS |
| AACAATAACAAAGATGGTGT+TGG | 0.562868 | 4.4:+22974442 | MS.gene32183:CDS |
| TATGCAGGTGGATATGGAGG+TGG | 0.563930 | 4.4:+22974562 | MS.gene32183:CDS |
| GACAACAACATCATAGGAGG+AGG | 0.563932 | 4.4:+22974532 | MS.gene32183:CDS |
| GGTAGTAACTGTTAGCAAGA+TGG | 0.568446 | 4.4:-22974356 | None:intergenic |
| AACAACATCATAGGAGGAGG+AGG | 0.572403 | 4.4:+22974535 | MS.gene32183:CDS |
| AGGCCCTTGTTACCAGAAGA+AGG | 0.575423 | 4.4:+22974654 | MS.gene32183:CDS |
| GGAGGAGGAGGAAATTATGC+AGG | 0.576032 | 4.4:+22974547 | MS.gene32183:CDS |
| GATATGGTGGTCCAAATGGA+GGG | 0.580834 | 4.4:+22974584 | MS.gene32183:CDS |
| TTGTTGTCAGCAGATGGTGA+AGG | 0.584431 | 4.4:-22974415 | None:intergenic |
| TCCTCCTGGTCCAAATCCAA+AGG | 0.585947 | 4.4:-22974488 | None:intergenic |
| GGTATGCTAAAACCTCCTCC+TGG | 0.595051 | 4.4:-22974502 | None:intergenic |
| AATTATGCAGGTGGATATGG+AGG | 0.596539 | 4.4:+22974559 | MS.gene32183:CDS |
| GGATATGGAGGTGGATATGG+TGG | 0.597908 | 4.4:+22974571 | MS.gene32183:CDS |
| TTTGACAACAACATCATAGG+AGG | 0.619262 | 4.4:+22974529 | MS.gene32183:CDS |
| ATGATGTTGTTGTCAAAACC+TGG | 0.622315 | 4.4:-22974523 | None:intergenic |
| AATAACAAAGATGGTGTTGG+TGG | 0.624778 | 4.4:+22974445 | MS.gene32183:CDS |
| AGAAGGTCACCTGTCCAGCA+AGG | 0.643768 | 4.4:+22974671 | MS.gene32183:CDS |
| GGTGGTGGCAATGACTATGG+TGG | 0.659694 | 4.4:+22974463 | MS.gene32183:CDS |
| TGACCTTCTTCTGGTAACAA+GGG | 0.660322 | 4.4:-22974657 | None:intergenic |
| ACTGTTGTCTGCAAAGACAG+AGG | 0.691072 | 4.4:+22974634 | MS.gene32183:CDS |
| TGTTGATGTGGATCTGCGAG+AGG | 0.712516 | 4.4:-22974386 | None:intergenic |
| GGATCTGCGAGAGGACACAG+AGG | 0.788957 | 4.4:-22974377 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAACAACAATAACAAAGA+TGG | + | chr4.4:22974436-22974455 | MS.gene32183:CDS | 25.0% |
| !! | ACATTTCTTTTTGCAATCAA+TGG | - | chr4.4:22974761-22974780 | None:intergenic | 25.0% |
| AACAATAACAAAGATGGTGT+TGG | + | chr4.4:22974442-22974461 | MS.gene32183:CDS | 30.0% | |
| ATGATGTTGTTGTCAAAACC+TGG | - | chr4.4:22974526-22974545 | None:intergenic | 35.0% | |
| TTTGACAACAACATCATAGG+AGG | + | chr4.4:22974529-22974548 | MS.gene32183:CDS | 35.0% | |
| ! | AATAACAAAGATGGTGTTGG+TGG | + | chr4.4:22974445-22974464 | MS.gene32183:CDS | 35.0% |
| !! | GGTTTTGACAACAACATCAT+AGG | + | chr4.4:22974526-22974545 | MS.gene32183:CDS | 35.0% |
| AAGGAAGTAAAACACCTTGC+TGG | - | chr4.4:22974688-22974707 | None:intergenic | 40.0% | |
| AATTATGCAGGTGGATATGG+AGG | + | chr4.4:22974559-22974578 | MS.gene32183:CDS | 40.0% | |
| GCAAGATGGTGAGCAAAATA+AGG | - | chr4.4:22974345-22974364 | None:intergenic | 40.0% | |
| GGAAATTATGCAGGTGGATA+TGG | + | chr4.4:22974556-22974575 | MS.gene32183:CDS | 40.0% | |
| GGTAGTAACTGTTAGCAAGA+TGG | - | chr4.4:22974359-22974378 | None:intergenic | 40.0% | |
| TGACCTTCTTCTGGTAACAA+GGG | - | chr4.4:22974660-22974679 | None:intergenic | 40.0% | |
| ! | TGAAGGTTTGGTTGTTGATG+TGG | - | chr4.4:22974401-22974420 | None:intergenic | 40.0% |
| ! | TTGTTGTTGTTGTCAGCAGA+TGG | - | chr4.4:22974424-22974443 | None:intergenic | 40.0% |
| AACAACATCATAGGAGGAGG+AGG | + | chr4.4:22974535-22974554 | MS.gene32183:CDS | 45.0% | |
| AATGACTATGGTGGCTCCTT+TGG | + | chr4.4:22974472-22974491 | MS.gene32183:CDS | 45.0% | |
| ACTGTTGTCTGCAAAGACAG+AGG | + | chr4.4:22974634-22974653 | MS.gene32183:CDS | 45.0% | |
| AGTAAAACACCTTGCTGGAC+AGG | - | chr4.4:22974683-22974702 | None:intergenic | 45.0% | |
| CTGTCTTTGCAGACAACAGT+TGG | - | chr4.4:22974634-22974653 | None:intergenic | 45.0% | |
| GACAACAACATCATAGGAGG+AGG | + | chr4.4:22974532-22974551 | MS.gene32183:CDS | 45.0% | |
| GATATGGTGGTCCAAATGGA+GGG | + | chr4.4:22974584-22974603 | MS.gene32183:CDS | 45.0% | |
| GTGACCTTCTTCTGGTAACA+AGG | - | chr4.4:22974661-22974680 | None:intergenic | 45.0% | |
| TATGGTGGCTCCTTTGGATT+TGG | + | chr4.4:22974478-22974497 | MS.gene32183:CDS | 45.0% | |
| TTGTTGTCAGCAGATGGTGA+AGG | - | chr4.4:22974418-22974437 | None:intergenic | 45.0% | |
| TTTACTTCCTTCAGTCGCTC+CGG | + | chr4.4:22974697-22974716 | MS.gene32183:CDS | 45.0% | |
| ! | TTTGTAGAGTGCCCTCCATT+TGG | - | chr4.4:22974598-22974617 | None:intergenic | 45.0% |
| !! | AACAAAGATGGTGTTGGTGG+TGG | + | chr4.4:22974448-22974467 | MS.gene32183:CDS | 45.0% |
| !! | AATGGAGGGCACTCTACAAA+TGG | + | chr4.4:22974598-22974617 | MS.gene32183:CDS | 45.0% |
| AGGCCCTTGTTACCAGAAGA+AGG | + | chr4.4:22974654-22974673 | MS.gene32183:CDS | 50.0% | |
| GGAGGAGGAAATTATGCAGG+TGG | + | chr4.4:22974550-22974569 | MS.gene32183:CDS | 50.0% | |
| GGAGGAGGAGGAAATTATGC+AGG | + | chr4.4:22974547-22974566 | MS.gene32183:CDS | 50.0% | |
| GGATATGGAGGTGGATATGG+TGG | + | chr4.4:22974571-22974590 | MS.gene32183:CDS | 50.0% | |
| GGATATGGTGGTCCAAATGG+AGG | + | chr4.4:22974583-22974602 | MS.gene32183:CDS | 50.0% | |
| GGTATGCTAAAACCTCCTCC+TGG | - | chr4.4:22974505-22974524 | None:intergenic | 50.0% | |
| GGTGGATATGGAGGTGGATA+TGG | + | chr4.4:22974568-22974587 | MS.gene32183:CDS | 50.0% | |
| GGTGGATATGGTGGTCCAAA+TGG | + | chr4.4:22974580-22974599 | MS.gene32183:CDS | 50.0% | |
| GTCAGCAGATGGTGAAGGTT+TGG | - | chr4.4:22974413-22974432 | None:intergenic | 50.0% | |
| TATGCAGGTGGATATGGAGG+TGG | + | chr4.4:22974562-22974581 | MS.gene32183:CDS | 50.0% | |
| TCCTCCTGGTCCAAATCCAA+AGG | - | chr4.4:22974491-22974510 | None:intergenic | 50.0% | |
| TCCTTTGGATTTGGACCAGG+AGG | + | chr4.4:22974487-22974506 | MS.gene32183:CDS | 50.0% | |
| TGTTGATGTGGATCTGCGAG+AGG | - | chr4.4:22974389-22974408 | None:intergenic | 50.0% | |
| TTTGGATTTGGACCAGGAGG+AGG | + | chr4.4:22974490-22974509 | MS.gene32183:CDS | 50.0% | |
| !! | GGAGGAGGTTTTAGCATACC+AGG | + | chr4.4:22974505-22974524 | MS.gene32183:CDS | 50.0% |
| !! | GTTGGTGGTGGCAATGACTA+TGG | + | chr4.4:22974460-22974479 | MS.gene32183:CDS | 50.0% |
| AGAAGGTCACCTGTCCAGCA+AGG | + | chr4.4:22974671-22974690 | MS.gene32183:CDS | 55.0% | |
| CTGGACAGGTGACCTTCTTC+TGG | - | chr4.4:22974669-22974688 | None:intergenic | 55.0% | |
| GGCTCCTTTGGATTTGGACC+AGG | + | chr4.4:22974484-22974503 | MS.gene32183:CDS | 55.0% | |
| ! | GGTGGTGGCAATGACTATGG+TGG | + | chr4.4:22974463-22974482 | MS.gene32183:CDS | 55.0% |
| GGATCTGCGAGAGGACACAG+AGG | - | chr4.4:22974380-22974399 | None:intergenic | 60.0% | |
| TCCTTCAGTCGCTCCGGCAA+GGG | + | chr4.4:22974703-22974722 | MS.gene32183:CDS | 60.0% | |
| TTCCTTCAGTCGCTCCGGCA+AGG | + | chr4.4:22974702-22974721 | MS.gene32183:CDS | 60.0% | |
| AGTCGCTCCGGCAAGGGCTA+CGG | + | chr4.4:22974709-22974728 | MS.gene32183:CDS | 65.0% | |
| GCCACCACCGTAGCCCTTGC+CGG | - | chr4.4:22974719-22974738 | None:intergenic | 70.0% | |
| GCCCTTGCCGGAGCGACTGA+AGG | - | chr4.4:22974707-22974726 | None:intergenic | 70.0% | |
| TCCGGCAAGGGCTACGGTGG+TGG | + | chr4.4:22974715-22974734 | MS.gene32183:CDS | 70.0% | |
| ! | GGCAAGGGCTACGGTGGTGG+CGG | + | chr4.4:22974718-22974737 | MS.gene32183:CDS | 70.0% |
| !! | AAGGGCTACGGTGGTGGCGG+TGG | + | chr4.4:22974721-22974740 | MS.gene32183:CDS | 70.0% |
| !! | TACGGTGGTGGCGGTGGAGG+TGG | + | chr4.4:22974727-22974746 | MS.gene32183:CDS | 70.0% |
| CGCTCCGGCAAGGGCTACGG+TGG | + | chr4.4:22974712-22974731 | MS.gene32183:CDS | 75.0% | |
| !! | GGCTACGGTGGTGGCGGTGG+AGG | + | chr4.4:22974724-22974743 | MS.gene32183:CDS | 75.0% |
| !! | GGTGGTGGCGGTGGAGGTGG+TGG | + | chr4.4:22974730-22974749 | MS.gene32183:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 22974325 | 22974795 | 22974325 | ID=MS.gene32183 |
| chr4.4 | mRNA | 22974325 | 22974795 | 22974325 | ID=MS.gene32183.t1;Parent=MS.gene32183 |
| chr4.4 | exon | 22974325 | 22974795 | 22974325 | ID=MS.gene32183.t1.exon1;Parent=MS.gene32183.t1 |
| chr4.4 | CDS | 22974325 | 22974795 | 22974325 | ID=cds.MS.gene32183.t1;Parent=MS.gene32183.t1 |
| Gene Sequence |
| Protein sequence |
