Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35147.t1 | XP_003608488.2 | 96.3 | 350 | 13 | 0 | 1 | 350 | 1 | 350 | 8.00E-185 | 656.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35147.t1 | Q402F4 | 23.4 | 295 | 166 | 8 | 21 | 310 | 38 | 277 | 1.2e-08 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35147.t1 | G7JDY4 | 96.3 | 350 | 13 | 0 | 1 | 350 | 1 | 350 | 5.8e-185 | 656.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene35147 | 0.813802 | 2.13E-51 | -1.69E-46 |
| MS.gene050277 | MS.gene35147 | 0.807201 | 5.67E-50 | -1.69E-46 |
| MS.gene051245 | MS.gene35147 | 0.825921 | 3.60E-54 | -1.69E-46 |
| MS.gene051662 | MS.gene35147 | 0.818486 | 1.91E-52 | -1.69E-46 |
| MS.gene051663 | MS.gene35147 | 0.801081 | 1.07E-48 | -1.69E-46 |
| MS.gene051746 | MS.gene35147 | 0.818468 | 1.93E-52 | -1.69E-46 |
| MS.gene051748 | MS.gene35147 | 0.812897 | 3.36E-51 | -1.69E-46 |
| MS.gene051789 | MS.gene35147 | 0.806797 | 6.91E-50 | -1.69E-46 |
| MS.gene052254 | MS.gene35147 | 0.800563 | 1.36E-48 | -1.69E-46 |
| MS.gene052256 | MS.gene35147 | 0.81539 | 9.48E-52 | -1.69E-46 |
| MS.gene052286 | MS.gene35147 | 0.802747 | 4.84E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene35147 | 0.830537 | 2.78E-55 | -1.69E-46 |
| MS.gene052309 | MS.gene35147 | -0.809941 | 1.47E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene35147 | 0.822781 | 1.97E-53 | -1.69E-46 |
| MS.gene052856 | MS.gene35147 | 0.815571 | 8.64E-52 | -1.69E-46 |
| MS.gene052858 | MS.gene35147 | 0.808472 | 3.05E-50 | -1.69E-46 |
| MS.gene053132 | MS.gene35147 | 0.807714 | 4.42E-50 | -1.69E-46 |
| MS.gene053437 | MS.gene35147 | 0.828355 | 9.41E-55 | -1.69E-46 |
| MS.gene053630 | MS.gene35147 | 0.812198 | 4.78E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene35147 | 0.807689 | 4.47E-50 | -1.69E-46 |
| MS.gene055174 | MS.gene35147 | 0.825413 | 4.75E-54 | -1.69E-46 |
| MS.gene055591 | MS.gene35147 | -0.836453 | 9.28E-57 | -1.69E-46 |
| MS.gene056069 | MS.gene35147 | -0.85806 | 1.07E-62 | -1.69E-46 |
| MS.gene057057 | MS.gene35147 | 0.832245 | 1.06E-55 | -1.69E-46 |
| MS.gene057234 | MS.gene35147 | 0.810562 | 1.08E-50 | -1.69E-46 |
| MS.gene057260 | MS.gene35147 | 0.817984 | 2.48E-52 | -1.69E-46 |
| MS.gene058182 | MS.gene35147 | 0.845279 | 4.48E-59 | -1.69E-46 |
| MS.gene058661 | MS.gene35147 | -0.805201 | 1.50E-49 | -1.69E-46 |
| MS.gene058851 | MS.gene35147 | 0.822915 | 1.83E-53 | -1.69E-46 |
| MS.gene058906 | MS.gene35147 | 0.822046 | 2.92E-53 | -1.69E-46 |
| MS.gene06001 | MS.gene35147 | 0.829131 | 6.11E-55 | -1.69E-46 |
| MS.gene060476 | MS.gene35147 | 0.821527 | 3.85E-53 | -1.69E-46 |
| MS.gene060619 | MS.gene35147 | 0.810496 | 1.12E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35147.t1 | MTR_4g096650 | 96.286 | 350 | 13 | 0 | 1 | 350 | 1 | 350 | 0.0 | 690 |
| MS.gene35147.t1 | MTR_4g096650 | 96.552 | 348 | 12 | 0 | 1 | 348 | 1 | 348 | 0.0 | 688 |
| MS.gene35147.t1 | MTR_4g096650 | 96.250 | 240 | 9 | 0 | 111 | 350 | 10 | 249 | 1.52e-171 | 478 |
| MS.gene35147.t1 | MTR_4g096650 | 96.639 | 238 | 8 | 0 | 111 | 348 | 10 | 247 | 5.29e-171 | 476 |
| MS.gene35147.t1 | MTR_5g091370 | 43.220 | 354 | 186 | 4 | 8 | 350 | 6 | 355 | 2.46e-84 | 260 |
| MS.gene35147.t1 | MTR_5g091370 | 43.172 | 227 | 117 | 2 | 8 | 226 | 6 | 228 | 9.82e-50 | 169 |
| MS.gene35147.t1 | MTR_4g094212 | 24.172 | 302 | 165 | 10 | 21 | 315 | 39 | 283 | 3.10e-13 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35147.t1 | AT3G59090 | 43.875 | 351 | 189 | 3 | 9 | 352 | 15 | 364 | 2.86e-88 | 271 |
| MS.gene35147.t1 | AT3G59090 | 43.966 | 348 | 187 | 3 | 9 | 349 | 15 | 361 | 9.39e-88 | 270 |
| MS.gene35147.t1 | AT3G59090 | 44.308 | 325 | 173 | 3 | 35 | 352 | 35 | 358 | 2.32e-83 | 258 |
| MS.gene35147.t1 | AT3G59090 | 42.904 | 303 | 165 | 3 | 57 | 352 | 1 | 302 | 1.06e-71 | 226 |
| MS.gene35147.t1 | AT4G21790 | 20.748 | 294 | 175 | 7 | 25 | 314 | 45 | 284 | 4.62e-12 | 66.6 |
Find 67 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGGTTACTCCATTTATT+TGG | 0.177458 | 4.4:-19392955 | MS.gene35147:intron |
| TATTGATTGGCTCTTCTAAT+TGG | 0.200988 | 4.4:-19395599 | MS.gene35147:CDS |
| TGCTTCCATTGCTTGTATTC+AGG | 0.201962 | 4.4:-19397013 | MS.gene35147:intron |
| ATTGATTGGCTCTTCTAATT+GGG | 0.264343 | 4.4:-19395598 | MS.gene35147:CDS |
| TACTTGGATATTTCTGCTAT+TGG | 0.264487 | 4.4:-19390081 | MS.gene35147:CDS |
| TGCTCATCTTCAAGCATAAT+AGG | 0.275987 | 4.4:+19389094 | None:intergenic |
| GAGTATCAATGCCAAATAAA+TGG | 0.302200 | 4.4:+19392944 | None:intergenic |
| TGAGAAACCTTCATCTGAAA+TGG | 0.328551 | 4.4:-19389061 | MS.gene35147:CDS |
| TGGCATTTGCTGTAATAATC+TGG | 0.345239 | 4.4:-19391072 | MS.gene35147:CDS |
| TTTCTCTGAGGAGCCTTTGC+TGG | 0.351840 | 4.4:-19391361 | MS.gene35147:CDS |
| CAGGTGTTTCATCTATTGAT+TGG | 0.362217 | 4.4:-19395612 | MS.gene35147:CDS |
| AGATCTAATGCAGGTGTACT+TGG | 0.364445 | 4.4:-19390097 | MS.gene35147:intron |
| AGCTACAAGCCTACAGAATC+AGG | 0.393975 | 4.4:-19388180 | MS.gene35147:intron |
| CCTTCAGCCATGGCGGGATT+CGG | 0.395466 | 4.4:-19397105 | None:intergenic |
| ACCTTTCCCATTTCAGATGA+AGG | 0.401135 | 4.4:+19389054 | None:intergenic |
| TTTGCTGTAATAATCTGGAT+TGG | 0.421058 | 4.4:-19391067 | MS.gene35147:CDS |
| CACTTGAGAAGTTCAGAGCT+TGG | 0.421460 | 4.4:-19396403 | MS.gene35147:CDS |
| CAGCCATGGCGGGATTCGGT+GGG | 0.427029 | 4.4:-19397101 | None:intergenic |
| GGCGCAGCACTGGCATGTTA+TGG | 0.442563 | 4.4:-19390045 | MS.gene35147:intron |
| TTGCTGTAATAATCTGGATT+GGG | 0.444019 | 4.4:-19391066 | MS.gene35147:CDS |
| CTTGCAAGGGATGGACATAT+TGG | 0.447221 | 4.4:-19392914 | MS.gene35147:CDS |
| TATTGCAGATGATGTTTCAC+TGG | 0.449421 | 4.4:-19388619 | MS.gene35147:intron |
| AGCTGCCACTTCAGAGTCAA+TGG | 0.450555 | 4.4:+19391030 | None:intergenic |
| GATACTCCTTGCTGCTTGCA+AGG | 0.453979 | 4.4:-19392928 | MS.gene35147:CDS |
| GAGAAACCTTCATCTGAAAT+GGG | 0.463494 | 4.4:-19389060 | MS.gene35147:CDS |
| TCAGCCATGGCGGGATTCGG+TGG | 0.465741 | 4.4:-19397102 | None:intergenic |
| TGACTCTGAAGTGGCAGCTC+GGG | 0.467323 | 4.4:-19391026 | MS.gene35147:intron |
| TTGATTGGCTCTTCTAATTG+GGG | 0.473554 | 4.4:-19395597 | MS.gene35147:intron |
| TTCTCTGAGGAGCCTTTGCT+GGG | 0.473938 | 4.4:-19391360 | MS.gene35147:CDS |
| GCCATGGCGGGATTCGGTGG+GGG | 0.481359 | 4.4:-19397099 | MS.gene35147:CDS |
| GTAATAATCTGGATTGGGCT+AGG | 0.488481 | 4.4:-19391061 | MS.gene35147:CDS |
| TCATAGTCATCGTCATCGTC+TGG | 0.494305 | 4.4:+19391390 | None:intergenic |
| CTGCTCATCATCCTCAGCGC+TGG | 0.496733 | 4.4:-19388208 | MS.gene35147:CDS |
| TGAGAAGTTCAGAGCTTGGT+TGG | 0.498394 | 4.4:-19396399 | MS.gene35147:CDS |
| CAACAACCTTCAGCCATGGC+GGG | 0.503548 | 4.4:-19397111 | None:intergenic |
| GTCCGTTAAAAGTTCTATGC+AGG | 0.507440 | 4.4:+19388735 | None:intergenic |
| TCCTTGCTGCTTGCAAGGGA+TGG | 0.511552 | 4.4:-19392923 | MS.gene35147:CDS |
| ATAACAGCGAAGAGTGGATA+CGG | 0.515410 | 4.4:+19397066 | None:intergenic |
| ACTACATCAGCAGCTTCTGC+TGG | 0.521458 | 4.4:+19388275 | None:intergenic |
| CATCGTCTGGCTGATGGCAT+AGG | 0.522540 | 4.4:+19391403 | None:intergenic |
| CATCGTCATCGTCTGGCTGA+TGG | 0.524421 | 4.4:+19391397 | None:intergenic |
| TTTGAAGTCTTTCCCAGCAA+AGG | 0.527404 | 4.4:+19391348 | None:intergenic |
| AGCCATGGCGGGATTCGGTG+GGG | 0.535819 | 4.4:-19397100 | None:intergenic |
| TTTGGGTACCCCAGTTGCTG+AGG | 0.538939 | 4.4:-19396429 | MS.gene35147:intron |
| ATACTCCTTGCTGCTTGCAA+GGG | 0.548818 | 4.4:-19392927 | MS.gene35147:CDS |
| CTATAGGGACCTGATTCTGT+AGG | 0.549428 | 4.4:+19388171 | None:intergenic |
| TTGACTCTGAAGTGGCAGCT+CGG | 0.554489 | 4.4:-19391027 | MS.gene35147:CDS |
| AATAACCTGAATACAAGCAA+TGG | 0.554655 | 4.4:+19397008 | None:intergenic |
| AGAAGCTGCTGATGTAGTAG+AGG | 0.555475 | 4.4:-19388270 | MS.gene35147:CDS |
| GCTGCCACTTCAGAGTCAAT+GGG | 0.557643 | 4.4:+19391031 | None:intergenic |
| GACGATGACGATGACTATGA+AGG | 0.575118 | 4.4:-19391387 | MS.gene35147:CDS |
| ACATATTGGTCACACTCTTG+TGG | 0.590797 | 4.4:-19392900 | MS.gene35147:CDS |
| GAATCCCATTGACTCTGAAG+TGG | 0.592710 | 4.4:-19391035 | MS.gene35147:CDS |
| ACCTTCAGCAGTAGTACTGT+GGG | 0.592883 | 4.4:-19388315 | MS.gene35147:CDS |
| ACCCACAGTACTACTGCTGA+AGG | 0.597180 | 4.4:+19388314 | None:intergenic |
| TACCTTCAGCAGTAGTACTG+TGG | 0.598958 | 4.4:-19388316 | MS.gene35147:CDS |
| ACCTTCATCTGAAATGGGAA+AGG | 0.606004 | 4.4:-19389055 | MS.gene35147:intron |
| ACAATGATAACAGCGAAGAG+TGG | 0.619986 | 4.4:+19397060 | None:intergenic |
| TCCATCCCTTGCAAGCAGCA+AGG | 0.625070 | 4.4:+19392922 | None:intergenic |
| ATACAAAATCAGCAGAAGAG+AGG | 0.630986 | 4.4:+19388568 | None:intergenic |
| TAACAGCGAAGAGTGGATAC+GGG | 0.634294 | 4.4:+19397067 | None:intergenic |
| ACATCAGCAGCTTCTGCTGG+CGG | 0.642156 | 4.4:+19388278 | None:intergenic |
| TATTACAGCAAATGCCACAA+TGG | 0.656668 | 4.4:+19391078 | None:intergenic |
| GAATCGCGTGAACAATAGCA+AGG | 0.665714 | 4.4:+19397037 | None:intergenic |
| AGAGTGGATACGGGAAACAG+TGG | 0.678735 | 4.4:+19397076 | None:intergenic |
| GTAGCTGTAGTCCAGCGCTG+AGG | 0.700160 | 4.4:+19388197 | None:intergenic |
| CTATGAAGGAAGTTTCTCTG+AGG | 0.700251 | 4.4:-19391373 | MS.gene35147:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 19387796 | 19397118 | 19387796 | ID=MS.gene35147 |
| chr4.4 | mRNA | 19387796 | 19397118 | 19387796 | ID=MS.gene35147.t1;Parent=MS.gene35147 |
| chr4.4 | exon | 19397014 | 19397118 | 19397014 | ID=MS.gene35147.t1.exon1;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19397014 | 19397118 | 19397014 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19396387 | 19396437 | 19396387 | ID=MS.gene35147.t1.exon2;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19396387 | 19396437 | 19396387 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19395598 | 19395631 | 19395598 | ID=MS.gene35147.t1.exon3;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19395598 | 19395631 | 19395598 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19392886 | 19392972 | 19392886 | ID=MS.gene35147.t1.exon4;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19392886 | 19392972 | 19392886 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19392746 | 19392797 | 19392746 | ID=MS.gene35147.t1.exon5;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19392746 | 19392797 | 19392746 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19391257 | 19391431 | 19391257 | ID=MS.gene35147.t1.exon6;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19391257 | 19391431 | 19391257 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19391027 | 19391122 | 19391027 | ID=MS.gene35147.t1.exon7;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19391027 | 19391122 | 19391027 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19390046 | 19390106 | 19390046 | ID=MS.gene35147.t1.exon8;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19390046 | 19390106 | 19390046 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19389056 | 19389120 | 19389056 | ID=MS.gene35147.t1.exon9;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19389056 | 19389120 | 19389056 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19388729 | 19388803 | 19388729 | ID=MS.gene35147.t1.exon10;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19388729 | 19388803 | 19388729 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19388558 | 19388633 | 19388558 | ID=MS.gene35147.t1.exon11;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19388558 | 19388633 | 19388558 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19388181 | 19388347 | 19388181 | ID=MS.gene35147.t1.exon12;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19388181 | 19388347 | 19388181 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| chr4.4 | exon | 19387796 | 19387858 | 19387796 | ID=MS.gene35147.t1.exon13;Parent=MS.gene35147.t1 |
| chr4.4 | CDS | 19387796 | 19387858 | 19387796 | ID=cds.MS.gene35147.t1;Parent=MS.gene35147.t1 |
| Gene Sequence |
| Protein sequence |
