AlfalfaGEDB | MS.gene41627

M. sativa cultivar XinJiangDaYe / MS.gene41627

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41627.t1	XP_003602727.1	89.8	108	2	2	1	108	1	99	2.40E-44	188

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41627.t1	B7FGG5	89.8	108	2	2	1	108	1	99	1.7e-44	188.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049181	MS.gene41627	0.859633	3.61E-63	-1.69E-46
MS.gene049182	MS.gene41627	0.813891	2.03E-51	-1.69E-46
MS.gene051959	MS.gene41627	0.825762	3.93E-54	-1.69E-46
MS.gene051960	MS.gene41627	0.832313	1.01E-55	-1.69E-46
MS.gene052091	MS.gene41627	0.813063	3.09E-51	-1.69E-46
MS.gene052793	MS.gene41627	0.800353	1.50E-48	-1.69E-46
MS.gene053083	MS.gene41627	0.820022	8.54E-53	-1.69E-46
MS.gene054413	MS.gene41627	0.805645	1.21E-49	-1.69E-46
MS.gene055150	MS.gene41627	0.854606	1.10E-61	-1.69E-46
MS.gene055549	MS.gene41627	0.818017	2.44E-52	-1.69E-46
MS.gene055550	MS.gene41627	0.814183	1.75E-51	-1.69E-46
MS.gene055634	MS.gene41627	-0.812574	3.96E-51	-1.69E-46
MS.gene055849	MS.gene41627	-0.810563	1.08E-50	-1.69E-46
MS.gene056530	MS.gene41627	0.821641	3.62E-53	-1.69E-46
MS.gene057001	MS.gene41627	0.802551	5.32E-49	-1.69E-46
MS.gene057148	MS.gene41627	-0.84064	7.70E-58	-1.69E-46
MS.gene057684	MS.gene41627	-0.803094	4.11E-49	-1.69E-46
MS.gene059268	MS.gene41627	0.81275	3.62E-51	-1.69E-46
MS.gene05985	MS.gene41627	-0.809012	2.33E-50	-1.69E-46
MS.gene060975	MS.gene41627	0.808862	2.51E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41627.t1	MTR_3g098390	89.815	108	2	2	1	108	1	99	1.11e-65	193

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41627.t1	AT2G44010	35.345	116	50	5	1	107	1	100	4.29e-12	57.8

Find 16 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTGTTGGAGATAATTT+TGG	0.176993	3.4:-88295487	MS.gene41627:CDS
CAGTATGAGGTTATTTCTTT+AGG	0.254934	3.4:-88295619	MS.gene41627:CDS
GCTTCAGATAATTACAATAT+TGG	0.370686	3.4:-88295523	MS.gene41627:CDS
TTTCATGATCAGACAACAAC+TGG	0.416618	3.4:-88295451	MS.gene41627:CDS
TAGAACAAGACGACAGTATG+AGG	0.483439	3.4:-88295632	MS.gene41627:CDS
TGAAGTGGTTGTTGAGTTTG+AGG	0.489679	3.4:+88295562	None:intergenic
CACAGGAGACATAAATCTGT+TGG	0.502696	3.4:-88295499	MS.gene41627:CDS
TATGAGGTTATTTCTTTAGG+AGG	0.514646	3.4:-88295616	MS.gene41627:CDS
GTGGTTGTTGAGTTTGAGGG+TGG	0.516517	3.4:+88295566	None:intergenic
GAAGTGGTTGTTGAGTTTGA+GGG	0.525781	3.4:+88295563	None:intergenic
ATATTGTAATTATCTGAAGC+TGG	0.533222	3.4:+88295526	None:intergenic
TTGATTGCTTTATACACAAG+AGG	0.566790	3.4:+88295661	None:intergenic
ATAATTACAATATTGGTCAC+AGG	0.578276	3.4:-88295516	MS.gene41627:CDS
GGTATTTGATTATGATGAAG+TGG	0.585229	3.4:+88295547	None:intergenic
ACAATAAGAAGTGTTATGGA+AGG	0.606553	3.4:-88295691	None:intergenic
AGGAAGCTACAACATAAGCA+TGG	0.662553	3.4:-88295596	MS.gene41627:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACAAGTTTTTTTGAAGAATT+AGG	+	chr3.4:88295633-88295652	None:intergenic	20.0%
!	ATAATTACAATATTGGTCAC+AGG	-	chr3.4:88295532-88295551	MS.gene41627:CDS	25.0%
!	ATTCTTCAAAAAAACTTGTC+AGG	-	chr3.4:88295634-88295653	MS.gene41627:CDS	25.0%
!	GCTTCAGATAATTACAATAT+TGG	-	chr3.4:88295525-88295544	MS.gene41627:CDS	25.0%
!!	AAATCTGTTGGAGATAATTT+TGG	-	chr3.4:88295561-88295580	MS.gene41627:CDS	25.0%
!!	AGAATGTTTTCATGTATCAA+GGG	-	chr3.4:88295669-88295688	MS.gene41627:CDS	25.0%
!!	ATATTGTAATTATCTGAAGC+TGG	+	chr3.4:88295525-88295544	None:intergenic	25.0%
!!	TAGAATGTTTTCATGTATCA+AGG	-	chr3.4:88295668-88295687	MS.gene41627:CDS	25.0%
	GGTATTTGATTATGATGAAG+TGG	+	chr3.4:88295504-88295523	None:intergenic	30.0%
	TTGATTGCTTTATACACAAG+AGG	+	chr3.4:88295390-88295409	None:intergenic	30.0%
!!	CAGTATGAGGTTATTTCTTT+AGG	-	chr3.4:88295429-88295448	MS.gene41627:CDS	30.0%
!!	GTTGGAGATAATTTTGGTAA+TGG	-	chr3.4:88295567-88295586	MS.gene41627:CDS	30.0%
!!	TATGAGGTTATTTCTTTAGG+AGG	-	chr3.4:88295432-88295451	MS.gene41627:CDS	30.0%
	TTTCATGATCAGACAACAAC+TGG	-	chr3.4:88295597-88295616	MS.gene41627:CDS	35.0%
	AGGAAGCTACAACATAAGCA+TGG	-	chr3.4:88295452-88295471	MS.gene41627:CDS	40.0%
	CACAGGAGACATAAATCTGT+TGG	-	chr3.4:88295549-88295568	MS.gene41627:CDS	40.0%
	GAAGTGGTTGTTGAGTTTGA+GGG	+	chr3.4:88295488-88295507	None:intergenic	40.0%
	TAGAACAAGACGACAGTATG+AGG	-	chr3.4:88295416-88295435	MS.gene41627:CDS	40.0%
	TGAAGTGGTTGTTGAGTTTG+AGG	+	chr3.4:88295489-88295508	None:intergenic	40.0%
	GTGGTTGTTGAGTTTGAGGG+TGG	+	chr3.4:88295485-88295504	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	88295372	88295698	88295372	ID=MS.gene41627
chr3.4	mRNA	88295372	88295698	88295372	ID=MS.gene41627.t1;Parent=MS.gene41627
chr3.4	exon	88295372	88295698	88295372	ID=MS.gene41627.t1.exon1;Parent=MS.gene41627.t1
chr3.4	CDS	88295372	88295698	88295372	ID=cds.MS.gene41627.t1;Parent=MS.gene41627.t1

Gene Sequence	>MS.gene41627 ATGGAAGGTCTTCTACCTCTTGTGTATAAAGCAATCAAGAAAAATAGAACAAGACGACAGTATGAGGTTATTTCTTTAGGAGGAAGCTACAACATAAGCATGGCTGAGATCCACCCTCAAACTCAACAACCACTTCATCATAATCAAATACCAGCTTCAGATAATTACAATATTGGTCACAGGAGACATAAATCTGTTGGAGATAATTTTGGTAATGGTTTTCAATTTCATGATCAGACAACAACTGGTTCTGTTTCTCCTAATTCTTCAAAAAAACTTGTCAGGTTTAGTAGTCATAGAATGTTTTCATGTATCAAGGGTGCCTGA
Protein sequence	>MS.gene41627.t1 MEGLLPLVYKAIKKNRTRRQYEVISLGGSYNISMAEIHPQTQQPLHHNQIPASDNYNIGHRRHKSVGDNFGNGFQFHDQTTTGSVSPNSSKKLVRFSSHRMFSCIKGA