Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43070.t1 | XP_013464930.1 | 97 | 67 | 2 | 0 | 1 | 67 | 1 | 67 | 3.40E-24 | 120.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43070.t1 | Q9SLF7 | 75.8 | 66 | 16 | 0 | 1 | 66 | 1 | 66 | 5.8e-20 | 98.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43070.t1 | A0A072VLF0 | 97.0 | 67 | 2 | 0 | 1 | 67 | 1 | 67 | 2.5e-24 | 120.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene43070 | 0.836939 | 6.97E-57 | -1.69E-46 |
| MS.gene050252 | MS.gene43070 | 0.848694 | 5.21E-60 | -1.69E-46 |
| MS.gene050290 | MS.gene43070 | 0.883606 | 3.89E-71 | -1.69E-46 |
| MS.gene050791 | MS.gene43070 | 0.871551 | 6.27E-67 | -1.69E-46 |
| MS.gene050912 | MS.gene43070 | 0.806672 | 7.34E-50 | -1.69E-46 |
| MS.gene051904 | MS.gene43070 | 0.852097 | 5.77E-61 | -1.69E-46 |
| MS.gene052664 | MS.gene43070 | 0.801522 | 8.65E-49 | -1.69E-46 |
| MS.gene053224 | MS.gene43070 | 0.83748 | 5.07E-57 | -1.69E-46 |
| MS.gene054937 | MS.gene43070 | 0.839695 | 1.36E-57 | -1.69E-46 |
| MS.gene056917 | MS.gene43070 | 0.865214 | 6.94E-65 | -1.69E-46 |
| MS.gene057052 | MS.gene43070 | 0.827072 | 1.91E-54 | -1.69E-46 |
| MS.gene057092 | MS.gene43070 | 0.819152 | 1.35E-52 | -1.69E-46 |
| MS.gene057272 | MS.gene43070 | 0.824425 | 8.13E-54 | -1.69E-46 |
| MS.gene060856 | MS.gene43070 | 0.823034 | 1.72E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTACTTGTTGGCCGTTT+TGG | 0.128749 | 2.4:+14173736 | MS.gene43070:CDS |
| GCTGATCTTAAACAAATTCT+TGG | 0.267351 | 2.4:+14173782 | MS.gene43070:CDS |
| GTTGCTGTGTCTGCTGCAAC+TGG | 0.362343 | 2.4:+14174986 | MS.gene43070:CDS |
| TCTTCCTTTGCTGCTTCAGC+AGG | 0.364467 | 2.4:-14175031 | None:intergenic |
| CTTAAACAAATTCTTGGATC+AGG | 0.372982 | 2.4:+14173788 | MS.gene43070:CDS |
| AGAGAAGAAGGTGGAAGAGA+AGG | 0.434796 | 2.4:+14175051 | MS.gene43070:CDS |
| GATTATCGCAGCTTACTTGT+TGG | 0.445399 | 2.4:+14173727 | MS.gene43070:CDS |
| GTGTCTGCTGCAACTGGAGG+AGG | 0.445905 | 2.4:+14174992 | MS.gene43070:CDS |
| TGCGCCTGCTGAAGCAGCAA+AGG | 0.454235 | 2.4:+14175027 | MS.gene43070:CDS |
| GATCAGCAGCTGAAGGGGAT+TGG | 0.469713 | 2.4:-14173766 | None:intergenic |
| CCTTGCCCTTGACTTCACTT+AGG | 0.488662 | 2.4:-14174898 | None:intergenic |
| TGCCAGTGGAAGAGAAAAGT+TGG | 0.491656 | 2.4:+14174937 | MS.gene43070:CDS |
| CACAGCAACAGCACCACCAC+CGG | 0.500128 | 2.4:-14174972 | None:intergenic |
| AGCAGCAAAGGAAGAGAAGA+AGG | 0.518126 | 2.4:+14175039 | MS.gene43070:CDS |
| GCTTCAGTTCCATCCGGTGG+TGG | 0.522209 | 2.4:+14174959 | MS.gene43070:CDS |
| GGATTGGTTGCCTCCCAAAA+CGG | 0.534645 | 2.4:-14173750 | None:intergenic |
| CAACTCAATGTTGCCATCCT+CGG | 0.536919 | 2.4:-14174873 | None:intergenic |
| GCAACAGCACCACCACCGGA+TGG | 0.539099 | 2.4:-14174968 | None:intergenic |
| TGTTTAAGATCAGCAGCTGA+AGG | 0.541739 | 2.4:-14173773 | None:intergenic |
| TCTTCCACTGGCAATAAGCT+CGG | 0.547245 | 2.4:-14174927 | None:intergenic |
| GTTTAAGATCAGCAGCTGAA+GGG | 0.564265 | 2.4:-14173772 | None:intergenic |
| TTTAAGATCAGCAGCTGAAG+GGG | 0.564880 | 2.4:-14173771 | None:intergenic |
| AAGTTGGCTTCAGTTCCATC+CGG | 0.567453 | 2.4:+14174953 | MS.gene43070:CDS |
| GTTGGAGTTGAGGCCGAGGA+TGG | 0.573673 | 2.4:+14174860 | MS.gene43070:intron |
| GTTGCTCCTAAGTGAAGTCA+AGG | 0.590863 | 2.4:+14174892 | MS.gene43070:CDS |
| GCTGTGTCTGCTGCAACTGG+AGG | 0.592795 | 2.4:+14174989 | MS.gene43070:CDS |
| TATGATTGTAGTTGGAGTTG+AGG | 0.599920 | 2.4:+14174850 | MS.gene43070:intron |
| TTTGCCGAGCTTATTGCCAG+TGG | 0.606365 | 2.4:+14174923 | MS.gene43070:CDS |
| TTGGCTTCAGTTCCATCCGG+TGG | 0.607108 | 2.4:+14174956 | MS.gene43070:CDS |
| CCTAAGTGAAGTCAAGGGCA+AGG | 0.616488 | 2.4:+14174898 | MS.gene43070:CDS |
| TGTAGTTGGAGTTGAGGCCG+AGG | 0.617745 | 2.4:+14174856 | MS.gene43070:intron |
| TTGCTCCTAAGTGAAGTCAA+GGG | 0.680870 | 2.4:+14174893 | MS.gene43070:CDS |
| AGCAAAGGAAGAGAAGAAGG+TGG | 0.683753 | 2.4:+14175042 | MS.gene43070:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAGATTAAAGATTAAAT+TGG | - | chr2.4:14173826-14173845 | None:intergenic | 10.0% |
| !! | ATAAGATTAAAGATTAAATT+GGG | - | chr2.4:14173825-14173844 | None:intergenic | 10.0% |
| !!! | AAATAATTTTAATTCAAGTT+TGG | + | chr2.4:14174219-14174238 | MS.gene43070:intron | 10.0% |
| !!! | AATAATTTTAATTCAAGTTT+GGG | + | chr2.4:14174220-14174239 | MS.gene43070:intron | 10.0% |
| !! | ATGAAAATTGATCAAAATAT+TGG | + | chr2.4:14174301-14174320 | MS.gene43070:intron | 15.0% |
| !! | ATTAAAATCAAACCAATATA+TGG | - | chr2.4:14174576-14174595 | None:intergenic | 15.0% |
| !! | TTAAAATCAAACCAATATAT+GGG | - | chr2.4:14174575-14174594 | None:intergenic | 15.0% |
| !!! | AAAAAAAGTGACTTTATTTT+TGG | + | chr2.4:14174824-14174843 | MS.gene43070:intron | 15.0% |
| !!! | AAAAAAGTGACTTTATTTTT+GGG | + | chr2.4:14174825-14174844 | MS.gene43070:intron | 15.0% |
| !!! | TTTAACTTTAATTCAAGTTT+GGG | + | chr2.4:14174253-14174272 | MS.gene43070:intron | 15.0% |
| !! | AATAGTTTATGCAAATTCAT+AGG | + | chr2.4:14174489-14174508 | MS.gene43070:intron | 20.0% |
| !! | ATGGATCTACTAAAATAATT+CGG | - | chr2.4:14173921-14173940 | None:intergenic | 20.0% |
| !! | TAAAATCAAACCAATATATG+GGG | - | chr2.4:14174574-14174593 | None:intergenic | 20.0% |
| !!! | AAATATTGGTTTATTTGGAT+TGG | + | chr2.4:14174315-14174334 | MS.gene43070:intron | 20.0% |
| !!! | ATATTCTACACTTTTACTTA+TGG | - | chr2.4:14173940-14173959 | None:intergenic | 20.0% |
| !!! | GATCAAAATATTGGTTTATT+TGG | + | chr2.4:14174310-14174329 | MS.gene43070:intron | 20.0% |
| !!! | GGATTTGTTTAATTGATAAT+CGG | + | chr2.4:14174088-14174107 | MS.gene43070:intron | 20.0% |
| !!! | GTATATTTTTTTACTGTTGA+AGG | + | chr2.4:14174711-14174730 | MS.gene43070:intron | 20.0% |
| !!! | GTTTAACTTTAATTCAAGTT+TGG | + | chr2.4:14174252-14174271 | MS.gene43070:intron | 20.0% |
| !!! | TGCTAATTTAATTCAAGTTT+AGG | + | chr2.4:14174067-14174086 | MS.gene43070:intron | 20.0% |
| !!! | TTCATTTTTACAGATGTATT+AGG | + | chr2.4:14174635-14174654 | MS.gene43070:intron | 20.0% |
| ! | TAAAATCGATGAATGAACAT+GGG | + | chr2.4:14174117-14174136 | MS.gene43070:intron | 25.0% |
| ! | TTAAAATCGATGAATGAACA+TGG | + | chr2.4:14174116-14174135 | MS.gene43070:intron | 25.0% |
| !! | TTTTACTGTTGAAGGAAAAA+TGG | + | chr2.4:14174719-14174738 | MS.gene43070:intron | 25.0% |
| !!! | ATTTTAATTCAAGTTTGGGT+TGG | + | chr2.4:14174224-14174243 | MS.gene43070:intron | 25.0% |
| !!! | GCTTAGTTTTATACATTGTT+TGG | + | chr2.4:14174596-14174615 | MS.gene43070:intron | 25.0% |
| !!! | TTTTAATTCAAGTTTGGGTT+GGG | + | chr2.4:14174225-14174244 | MS.gene43070:intron | 25.0% |
| AAATAATCAATCCTAGACTG+TGG | + | chr2.4:14174022-14174041 | MS.gene43070:intron | 30.0% | |
| AATAATCAATCCTAGACTGT+GGG | + | chr2.4:14174023-14174042 | MS.gene43070:intron | 30.0% | |
| CTTAAACAAATTCTTGGATC+AGG | + | chr2.4:14173788-14173807 | MS.gene43070:CDS | 30.0% | |
| GCTGATCTTAAACAAATTCT+TGG | + | chr2.4:14173782-14173801 | MS.gene43070:CDS | 30.0% | |
| TATCTACTGTTAGACTGTTA+AGG | + | chr2.4:14174351-14174370 | MS.gene43070:intron | 30.0% | |
| !!! | GCATTTTTTGTTCGTTTTTC+CGG | + | chr2.4:14173879-14173898 | MS.gene43070:intron | 30.0% |
| AATCAAACAGCCCCATATAT+TGG | + | chr2.4:14174561-14174580 | MS.gene43070:intron | 35.0% | |
| TATGATTGTAGTTGGAGTTG+AGG | + | chr2.4:14174850-14174869 | MS.gene43070:intron | 35.0% | |
| TCGTTTGAGCTTAACTGTTT+GGG | + | chr2.4:14174156-14174175 | MS.gene43070:intron | 35.0% | |
| TTTGGGTCTATGATTGTAGT+TGG | + | chr2.4:14174842-14174861 | MS.gene43070:intron | 35.0% | |
| CGGATACTAATCATCGAAAC+CGG | - | chr2.4:14173901-14173920 | None:intergenic | 40.0% | |
| CTCGTTTGAGCTTAACTGTT+TGG | + | chr2.4:14174155-14174174 | MS.gene43070:intron | 40.0% | |
| CTTCATTATACCCACAGTCT+AGG | - | chr2.4:14174036-14174055 | None:intergenic | 40.0% | |
| GATTATCGCAGCTTACTTGT+TGG | + | chr2.4:14173727-14173746 | MS.gene43070:CDS | 40.0% | |
| TGAATGAACATGGGTCTGTT+TGG | + | chr2.4:14174126-14174145 | MS.gene43070:intron | 40.0% | |
| TTGCTCCTAAGTGAAGTCAA+GGG | + | chr2.4:14174893-14174912 | MS.gene43070:CDS | 40.0% | |
| ! | GTTTAAGATCAGCAGCTGAA+GGG | - | chr2.4:14173775-14173794 | None:intergenic | 40.0% |
| ! | TGTTTAAGATCAGCAGCTGA+AGG | - | chr2.4:14173776-14173795 | None:intergenic | 40.0% |
| ! | TTTAAGATCAGCAGCTGAAG+GGG | - | chr2.4:14173774-14173793 | None:intergenic | 40.0% |
| AGAGAAGAAGGTGGAAGAGA+AGG | + | chr2.4:14175051-14175070 | MS.gene43070:CDS | 45.0% | |
| AGCAAAGGAAGAGAAGAAGG+TGG | + | chr2.4:14175042-14175061 | MS.gene43070:CDS | 45.0% | |
| AGCAGCAAAGGAAGAGAAGA+AGG | + | chr2.4:14175039-14175058 | MS.gene43070:CDS | 45.0% | |
| CAACTCAATGTTGCCATCCT+CGG | - | chr2.4:14174876-14174895 | None:intergenic | 45.0% | |
| GAACATGGGTCTGTTTGGAT+TGG | + | chr2.4:14174131-14174150 | MS.gene43070:intron | 45.0% | |
| GTTGCTCCTAAGTGAAGTCA+AGG | + | chr2.4:14174892-14174911 | MS.gene43070:CDS | 45.0% | |
| TCTTCCACTGGCAATAAGCT+CGG | - | chr2.4:14174930-14174949 | None:intergenic | 45.0% | |
| TGCCAGTGGAAGAGAAAAGT+TGG | + | chr2.4:14174937-14174956 | MS.gene43070:CDS | 45.0% | |
| ! | AAGTTGGCTTCAGTTCCATC+CGG | + | chr2.4:14174953-14174972 | MS.gene43070:CDS | 45.0% |
| ! | AGCCAACTTTTCTCTTCCAC+TGG | - | chr2.4:14174942-14174961 | None:intergenic | 45.0% |
| !! | AGCTTACTTGTTGGCCGTTT+TGG | + | chr2.4:14173736-14173755 | MS.gene43070:CDS | 45.0% |
| !! | GCTTACTTGTTGGCCGTTTT+GGG | + | chr2.4:14173737-14173756 | MS.gene43070:CDS | 45.0% |
| CCTAAGTGAAGTCAAGGGCA+AGG | + | chr2.4:14174898-14174917 | MS.gene43070:CDS | 50.0% | |
| CCTTGCCCTTGACTTCACTT+AGG | - | chr2.4:14174901-14174920 | None:intergenic | 50.0% | |
| TTTGCCGAGCTTATTGCCAG+TGG | + | chr2.4:14174923-14174942 | MS.gene43070:CDS | 50.0% | |
| ! | TCTTCCTTTGCTGCTTCAGC+AGG | - | chr2.4:14175034-14175053 | None:intergenic | 50.0% |
| !! | GGATTGGTTGCCTCCCAAAA+CGG | - | chr2.4:14173753-14173772 | None:intergenic | 50.0% |
| !! | TACTTGTTGGCCGTTTTGGG+AGG | + | chr2.4:14173740-14173759 | MS.gene43070:CDS | 50.0% |
| GATCAGCAGCTGAAGGGGAT+TGG | - | chr2.4:14173769-14173788 | None:intergenic | 55.0% | |
| GTTGCTGTGTCTGCTGCAAC+TGG | + | chr2.4:14174986-14175005 | MS.gene43070:CDS | 55.0% | |
| TGTAGTTGGAGTTGAGGCCG+AGG | + | chr2.4:14174856-14174875 | MS.gene43070:intron | 55.0% | |
| TTGGCTTCAGTTCCATCCGG+TGG | + | chr2.4:14174956-14174975 | MS.gene43070:CDS | 55.0% | |
| CACAGCAACAGCACCACCAC+CGG | - | chr2.4:14174975-14174994 | None:intergenic | 60.0% | |
| GCTGTGTCTGCTGCAACTGG+AGG | + | chr2.4:14174989-14175008 | MS.gene43070:CDS | 60.0% | |
| GCTTCAGTTCCATCCGGTGG+TGG | + | chr2.4:14174959-14174978 | MS.gene43070:CDS | 60.0% | |
| GTGTCTGCTGCAACTGGAGG+AGG | + | chr2.4:14174992-14175011 | MS.gene43070:CDS | 60.0% | |
| GTTGGAGTTGAGGCCGAGGA+TGG | + | chr2.4:14174860-14174879 | MS.gene43070:intron | 60.0% | |
| TGCGCCTGCTGAAGCAGCAA+AGG | + | chr2.4:14175027-14175046 | MS.gene43070:CDS | 60.0% | |
| GCAACAGCACCACCACCGGA+TGG | - | chr2.4:14174971-14174990 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 14173720 | 14175087 | 14173720 | ID=MS.gene43070 |
| chr2.4 | mRNA | 14173720 | 14175087 | 14173720 | ID=MS.gene43070.t1;Parent=MS.gene43070 |
| chr2.4 | exon | 14173720 | 14173809 | 14173720 | ID=MS.gene43070.t1.exon1;Parent=MS.gene43070.t1 |
| chr2.4 | CDS | 14173720 | 14173809 | 14173720 | ID=cds.MS.gene43070.t1;Parent=MS.gene43070.t1 |
| chr2.4 | exon | 14174861 | 14175087 | 14174861 | ID=MS.gene43070.t1.exon2;Parent=MS.gene43070.t1 |
| chr2.4 | CDS | 14174861 | 14175087 | 14174861 | ID=cds.MS.gene43070.t1;Parent=MS.gene43070.t1 |
| Gene Sequence |
| Protein sequence |
