AlfalfaGEDB | MS.gene43439

M. sativa cultivar XinJiangDaYe / MS.gene43439

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43439.t1	RHN65062.1	91.6	251	21	0	1	251	1	251	1.20E-128	469.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43439.t1	Q9SRH9	40.3	149	81	2	11	151	56	204	3.6e-20	100.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43439.t1	G7JLN1	91.6	251	21	0	1	251	1	251	8.9e-129	469.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene43439.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene80087	MS.gene43439	PPI
MS.gene93863	MS.gene43439	PPI
MS.gene43439	MS.gene93863	PPI
MS.gene43439	MS.gene93862	PPI
MS.gene012752	MS.gene43439	PPI
MS.gene80088	MS.gene43439	PPI
MS.gene91421	MS.gene43439	PPI
MS.gene93864	MS.gene43439	PPI
MS.gene43439	MS.gene71600	PPI
MS.gene85574	MS.gene43439	PPI
MS.gene99643	MS.gene43439	PPI
MS.gene95089	MS.gene43439	PPI
MS.gene93862	MS.gene43439	PPI
MS.gene99644	MS.gene43439	PPI
MS.gene43439	MS.gene91422	PPI
MS.gene91330	MS.gene43439	PPI
MS.gene80089	MS.gene43439	PPI
MS.gene60770	MS.gene43439	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43439.t1	MTR_4g133300	91.633	251	21	0	1	251	1	251	1.60e-171	473
MS.gene43439.t1	MTR_4g133470	90.837	251	23	0	1	251	1	251	2.13e-169	467
MS.gene43439.t1	MTR_2g097830	93.578	109	7	0	1	109	59	167	4.17e-71	215
MS.gene43439.t1	MTR_2g097830	34.286	140	81	4	56	192	36	167	4.41e-12	63.2
MS.gene43439.t1	MTR_2g097830	45.588	68	34	1	175	242	73	137	6.24e-11	59.7
MS.gene43439.t1	MTR_1g104960	42.767	159	88	1	89	244	13	171	1.70e-34	122
MS.gene43439.t1	MTR_1g104960	43.275	171	85	4	1	160	1	170	1.17e-31	115
MS.gene43439.t1	MTR_3g006125	42.328	189	98	5	66	245	68	254	1.06e-32	120
MS.gene43439.t1	MTR_3g006125	43.056	144	71	2	25	157	106	249	1.86e-27	106
MS.gene43439.t1	MTR_3g006185	45.614	171	85	4	83	245	2	172	1.09e-32	117
MS.gene43439.t1	MTR_3g006185	41.611	149	77	2	25	163	25	173	5.46e-27	102
MS.gene43439.t1	MTR_3g006150	45.161	155	79	3	10	158	6	160	1.55e-32	117
MS.gene43439.t1	MTR_3g006150	46.497	157	76	5	89	241	8	160	7.19e-30	110
MS.gene43439.t1	MTR_3g006065	46.405	153	76	3	12	158	8	160	2.07e-32	117
MS.gene43439.t1	MTR_3g006065	42.105	171	93	3	83	250	2	169	5.40e-29	108
MS.gene43439.t1	MTR_3g006065	51.786	56	27	0	25	80	107	162	1.01e-12	65.1
MS.gene43439.t1	MTR_7g013380	33.913	230	141	5	25	244	31	259	2.66e-32	120
MS.gene43439.t1	MTR_7g013380	34.783	161	98	4	90	244	14	173	4.91e-20	87.4
MS.gene43439.t1	MTR_1g105955	41.772	158	78	2	88	240	15	163	1.63e-30	112
MS.gene43439.t1	MTR_1g105955	44.595	148	72	3	13	154	17	160	1.61e-27	104
MS.gene43439.t1	MTR_1g105955	47.222	72	35	1	8	76	91	162	1.81e-13	67.0
MS.gene43439.t1	MTR_3g006105	43.114	167	86	4	83	240	2	168	9.83e-29	107
MS.gene43439.t1	MTR_3g006105	42.282	149	74	4	25	162	25	172	5.92e-28	105
MS.gene43439.t1	MTR_1g104980	40.789	152	78	2	93	244	3	142	3.62e-27	103
MS.gene43439.t1	MTR_1g104980	41.259	143	75	2	25	167	15	148	2.59e-23	93.2
MS.gene43439.t1	MTR_1g109550	40.845	142	79	2	1	140	5	143	3.18e-26	100
MS.gene43439.t1	MTR_1g109550	36.296	135	73	4	89	220	16	140	8.22e-17	75.1
MS.gene43439.t1	MTR_1g109550	47.692	65	33	1	175	239	19	82	2.43e-13	65.9
MS.gene43439.t1	MTR_5g036670	40.000	160	87	2	2	152	33	192	2.64e-25	104
MS.gene43439.t1	MTR_5g036670	35.185	162	100	2	79	235	31	192	1.78e-22	96.7
MS.gene43439.t1	MTR_5g036670	49.398	83	40	1	164	244	33	115	7.25e-17	80.1
MS.gene43439.t1	MTR_8g062700	39.766	171	86	3	11	164	41	211	1.19e-24	102
MS.gene43439.t1	MTR_8g062700	35.256	156	98	2	83	235	36	191	1.75e-20	90.9
MS.gene43439.t1	MTR_8g062700	52.564	78	35	1	167	242	35	112	1.72e-16	79.0
MS.gene43439.t1	MTR_8g062700	39.766	171	86	3	11	164	41	211	1.19e-24	102
MS.gene43439.t1	MTR_8g062700	35.256	156	98	2	83	235	36	191	1.73e-20	90.9
MS.gene43439.t1	MTR_8g062700	52.564	78	35	1	167	242	35	112	1.72e-16	79.0
MS.gene43439.t1	MTR_6g008970	39.375	160	87	3	3	152	53	212	1.41e-22	97.1
MS.gene43439.t1	MTR_6g008970	34.177	158	101	1	81	235	55	212	1.99e-19	87.8
MS.gene43439.t1	MTR_6g008970	51.948	77	35	1	167	241	56	132	3.83e-15	75.1
MS.gene43439.t1	MTR_6g008995	38.514	148	84	2	12	152	37	184	4.74e-20	89.0
MS.gene43439.t1	MTR_6g008995	35.484	155	95	3	84	235	32	184	2.89e-18	84.0
MS.gene43439.t1	MTR_6g008995	59.701	67	27	0	175	241	40	106	3.86e-16	77.8
MS.gene43439.t1	MTR_7g013410	33.571	140	89	2	16	151	2	141	2.16e-19	82.0
MS.gene43439.t1	MTR_7g013410	28.873	142	99	1	93	234	2	141	8.84e-13	64.3
MS.gene43439.t1	MTR_7g013410	44.776	67	37	0	176	242	2	68	6.95e-12	62.0
MS.gene43439.t1	MTR_6g008995	38.889	126	73	1	31	152	2	127	2.01e-18	84.0
MS.gene43439.t1	MTR_6g008995	32.812	128	81	3	111	235	2	127	3.46e-11	62.8
MS.gene43439.t1	MTR_6g008995	64.583	48	17	0	194	241	2	49	4.67e-11	62.4
MS.gene43439.t1	MTR_1g105960	53.030	66	31	0	175	240	4	69	3.87e-16	72.4
MS.gene43439.t1	MTR_1g105960	39.130	115	47	2	13	124	2	96	1.09e-14	68.6
MS.gene43439.t1	MTR_1g105960	35.294	119	54	1	90	208	2	97	5.03e-14	66.6
MS.gene43439.t1	MTR_1g105010	49.315	73	37	0	85	157	12	84	4.58e-15	69.7
MS.gene43439.t1	MTR_1g105010	40.244	82	46	1	162	240	3	84	1.03e-12	63.5
MS.gene43439.t1	MTR_8g062350	39.785	93	54	1	83	175	8	98	5.84e-15	71.6
MS.gene43439.t1	MTR_8g062350	43.023	86	42	2	167	250	7	87	5.04e-11	60.8
MS.gene43439.t1	MTR_1g104940	47.368	76	40	0	175	250	17	92	8.97e-15	69.3
MS.gene43439.t1	MTR_1g104940	40.698	86	51	0	89	174	14	99	1.99e-13	65.5
MS.gene43439.t1	MTR_1g104940	50.000	60	30	0	25	84	30	89	1.00e-11	60.8
MS.gene43439.t1	MTR_1g104937	36.364	99	63	0	89	187	12	110	3.52e-13	64.7
MS.gene43439.t1	MTR_1g104937	42.857	70	40	0	175	244	15	84	5.46e-12	61.2
MS.gene43439.t1	MTR_1g093310	46.667	75	34	1	175	249	12	80	5.74e-13	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43439.t1	AT1G24190	39.735	151	82	2	11	152	13	163	6.53e-25	103
MS.gene43439.t1	AT1G24190	32.919	161	99	2	81	235	6	163	3.26e-19	87.0
MS.gene43439.t1	AT1G24190	54.286	70	32	0	175	244	17	86	1.84e-14	73.2
MS.gene43439.t1	AT1G24190	39.735	151	82	2	11	152	13	163	6.53e-25	103
MS.gene43439.t1	AT1G24190	32.919	161	99	2	81	235	6	163	3.33e-19	87.0
MS.gene43439.t1	AT1G24190	54.286	70	32	0	175	244	17	86	1.81e-14	73.2
MS.gene43439.t1	AT1G24190	39.735	151	82	2	11	152	13	163	8.30e-25	103
MS.gene43439.t1	AT1G24190	32.919	161	99	2	81	235	6	163	3.49e-19	87.0
MS.gene43439.t1	AT1G24190	54.286	70	32	0	175	244	17	86	1.97e-14	73.2
MS.gene43439.t1	AT1G70060	38.750	160	88	2	3	152	5	164	2.30e-23	99.4
MS.gene43439.t1	AT1G70060	33.333	159	102	1	81	235	6	164	4.27e-18	84.0
MS.gene43439.t1	AT1G70060	55.224	67	30	0	175	241	17	83	7.93e-14	71.2
MS.gene43439.t1	AT1G70060	38.750	160	88	2	3	152	5	164	2.30e-23	99.4
MS.gene43439.t1	AT1G70060	33.333	159	102	1	81	235	6	164	4.27e-18	84.0
MS.gene43439.t1	AT1G70060	55.224	67	30	0	175	241	17	83	7.93e-14	71.2
MS.gene43439.t1	AT3G01320	40.268	149	81	2	11	151	56	204	5.55e-23	98.2
MS.gene43439.t1	AT3G01320	33.766	154	100	1	83	234	51	204	3.29e-18	84.3
MS.gene43439.t1	AT3G01320	54.545	77	33	1	167	241	50	126	2.35e-17	81.6
MS.gene43439.t1	AT3G01320	40.268	149	81	2	11	151	56	204	6.77e-23	97.8
MS.gene43439.t1	AT3G01320	33.766	154	100	1	83	234	51	204	4.01e-18	84.0
MS.gene43439.t1	AT3G01320	54.545	77	33	1	167	241	50	126	2.70e-17	81.6
MS.gene43439.t1	AT5G15020	38.926	149	84	2	11	152	51	199	3.28e-22	95.9
MS.gene43439.t1	AT5G15020	34.416	154	100	1	83	235	46	199	7.68e-20	89.0
MS.gene43439.t1	AT5G15020	51.316	76	35	1	168	241	46	121	5.45e-15	74.7
MS.gene43439.t1	AT5G15020	38.926	149	84	2	11	152	51	199	3.54e-22	95.9
MS.gene43439.t1	AT5G15020	34.416	154	100	1	83	235	46	199	8.22e-20	89.0
MS.gene43439.t1	AT5G15020	51.316	76	35	1	168	241	46	121	6.17e-15	74.7
MS.gene43439.t1	AT1G59890	39.752	161	87	3	12	162	45	205	4.21e-22	95.5
MS.gene43439.t1	AT1G59890	37.162	148	92	1	89	235	45	192	7.48e-19	86.3
MS.gene43439.t1	AT1G59890	50.746	67	33	0	175	241	48	114	9.33e-13	68.2
MS.gene43439.t1	AT1G59890	39.752	161	87	3	12	162	45	205	4.30e-22	95.5
MS.gene43439.t1	AT1G59890	37.162	148	92	1	89	235	45	192	7.42e-19	86.3
MS.gene43439.t1	AT1G59890	50.746	67	33	0	175	241	48	114	9.43e-13	68.2
MS.gene43439.t1	AT1G59890	39.752	161	87	3	12	162	45	205	4.41e-22	95.5
MS.gene43439.t1	AT1G59890	37.162	148	92	1	89	235	45	192	8.37e-19	85.9
MS.gene43439.t1	AT1G59890	50.746	67	33	0	175	241	48	114	1.04e-12	67.8
MS.gene43439.t1	AT1G59890	39.752	161	87	3	12	162	45	205	4.41e-22	95.5
MS.gene43439.t1	AT1G59890	37.162	148	92	1	89	235	45	192	7.55e-19	85.9
MS.gene43439.t1	AT1G59890	50.746	67	33	0	175	241	48	114	9.77e-13	68.2
MS.gene43439.t1	AT1G24230	33.758	157	91	3	89	244	14	158	2.30e-20	87.4
MS.gene43439.t1	AT1G24230	27.111	225	149	4	11	230	13	227	2.81e-19	84.3
MS.gene43439.t1	AT1G10450	38.776	147	83	2	12	151	84	230	2.48e-20	90.5
MS.gene43439.t1	AT1G10450	37.838	148	89	2	89	234	84	230	1.74e-19	87.8
MS.gene43439.t1	AT1G10450	49.254	67	34	0	175	241	87	153	9.89e-13	68.2
MS.gene43439.t1	AT1G10450	38.776	147	83	2	12	151	33	179	3.27e-20	90.1
MS.gene43439.t1	AT1G10450	37.838	148	89	2	89	234	33	179	1.63e-19	88.2
MS.gene43439.t1	AT1G10450	49.254	67	34	0	175	241	36	102	1.00e-12	68.2
MS.gene43439.t1	AT1G10450	38.776	147	83	2	12	151	33	179	3.27e-20	90.1
MS.gene43439.t1	AT1G10450	37.838	148	89	2	89	234	33	179	1.63e-19	88.2
MS.gene43439.t1	AT1G10450	49.254	67	34	0	175	241	36	102	1.00e-12	68.2
MS.gene43439.t1	AT1G27260	34.615	156	91	3	90	236	4	157	3.27e-18	80.9
MS.gene43439.t1	AT1G27260	31.210	157	95	2	13	156	4	160	9.27e-15	71.6
MS.gene43439.t1	AT1G24250	30.270	185	100	4	2	157	4	188	5.64e-17	78.2
MS.gene43439.t1	AT1G24250	28.235	170	97	4	88	233	13	181	2.80e-12	65.1
MS.gene43439.t1	AT1G70030	34.416	154	87	5	12	155	11	160	1.07e-16	75.5
MS.gene43439.t1	AT1G70030	33.333	159	97	4	81	234	3	157	2.75e-15	71.6
MS.gene43439.t1	AT1G23810	30.508	177	105	3	2	160	4	180	1.41e-16	77.0
MS.gene43439.t1	AT1G23810	29.518	166	103	4	88	240	13	177	1.54e-13	68.6
MS.gene43439.t1	AT1G24200	35.570	149	90	2	89	234	14	159	1.46e-16	75.9
MS.gene43439.t1	AT1G24200	34.694	147	90	2	11	151	13	159	6.35e-16	74.3
MS.gene43439.t1	AT1G24200	46.053	76	39	1	167	240	7	82	8.34e-13	65.9
MS.gene43439.t1	AT1G27280	34.014	147	92	2	100	241	4	150	2.60e-15	73.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.53e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	4.88e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.90e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	5.09e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.90e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	5.09e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.91e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	5.09e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.91e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	5.10e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.91e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	5.10e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	245	309	8.92e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	241	343	5.10e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.95e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	5.11e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.95e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	5.12e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.96e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	5.12e-11	63.2
MS.gene43439.t1	AT4G12020	51.515	66	31	1	176	241	312	376	8.96e-13	68.2
MS.gene43439.t1	AT4G12020	35.345	116	56	3	12	121	308	410	5.12e-11	63.2
MS.gene43439.t1	AT5G15025	50.746	67	33	0	175	241	50	116	1.17e-12	63.5
MS.gene43439.t1	AT5G15025	45.349	86	43	2	2	83	36	121	5.82e-12	61.6
MS.gene43439.t1	AT1G70030	33.333	147	66	6	12	155	22	139	5.47e-12	62.0
MS.gene43439.t1	AT5G35610	40.244	82	42	1	176	250	14	95	2.35e-11	60.8
MS.gene43439.t1	AT5G15040	44.776	67	37	0	175	241	6	72	2.83e-11	58.9
MS.gene43439.t1	AT5G15040	44.776	67	37	0	175	241	6	72	2.83e-11	58.9
MS.gene43439.t1	AT5G15040	44.776	67	37	0	175	241	6	72	2.83e-11	58.9
MS.gene43439.t1	AT1G27250	46.154	65	35	0	175	239	24	88	4.09e-11	59.7
MS.gene43439.t1	AT1G27270	32.164	171	93	4	10	157	12	182	5.04e-11	61.6
MS.gene43439.t1	AT1G24220	25.506	247	136	8	3	224	5	228	5.75e-11	62.8
MS.gene43439.t1	AT1G24220	25.506	247	136	8	3	224	5	228	5.75e-11	62.8

Find 37 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCCCAAAATTAAATGTTTA+TGG	0.087759	8.4:+656758	None:intergenic
AGACCATACAAATTTAATTT+TGG	0.091325	8.4:-656272	MS.gene43439:CDS
AGGCCATAAACATTTAATTT+TGG	0.093733	8.4:-656761	MS.gene43439:CDS
AATGTGAATCCGTATCTTTC+TGG	0.260796	8.4:+656481	None:intergenic
GGGCATGTCGATTTACTTTC+GGG	0.267983	8.4:-656520	MS.gene43439:CDS
ATTGCGATGAAGCTATTTAA+AGG	0.296597	8.4:-656541	MS.gene43439:CDS
AGTATGATTTCATAATTCTT+TGG	0.310501	8.4:+656721	None:intergenic
AGAGTGCATGAGTTGTTTAA+AGG	0.320962	8.4:-656781	MS.gene43439:CDS
AGGGCATGTCGATTTACTTT+CGG	0.325603	8.4:-656521	MS.gene43439:CDS
CAGAAGTCATGAGTGATTAC+AGG	0.353321	8.4:-656837	MS.gene43439:CDS
ATCCCAAAATTAAATTTGTA+TGG	0.371343	8.4:+656269	None:intergenic
TTGCGATGAAGCTATTTAAA+GGG	0.398600	8.4:-656540	MS.gene43439:CDS
ACTCTTGCTTTGACACCTCT+TGG	0.426483	8.4:+656799	None:intergenic
TCATGGCAATGAAGCTCAAA+TGG	0.432218	8.4:+656208	None:intergenic
AGTTGTGTTTCAAGATAAGA+TGG	0.435039	8.4:-656638	MS.gene43439:CDS
GAGCTTCATTGCCATGAGAC+TGG	0.471478	8.4:-656202	MS.gene43439:CDS
AGCCGTGTTTCATGATAAGT+TGG	0.480965	8.4:-656389	MS.gene43439:CDS
AGTGTGATTTGATGTTCCTC+TGG	0.511878	8.4:+656232	None:intergenic
TACTTCTTGCCAGAAAGATA+CGG	0.529766	8.4:-656490	MS.gene43439:CDS
CAAGGCTCAAGGAATTGATA+CGG	0.538557	8.4:-656329	MS.gene43439:CDS
TGTTTATGATCTTGCAACAG+TGG	0.557427	8.4:+656457	None:intergenic
GATGCTTTGTTTGATGATGC+AGG	0.558023	8.4:-656913	MS.gene43439:CDS
TGAAATCATACTTCCTCCAT+CGG	0.558415	8.4:-656710	MS.gene43439:CDS
AATTGATACGGGAGGTGTTG+CGG	0.560033	8.4:-656317	MS.gene43439:CDS
GTCATGAGTGATTACAGGGA+TGG	0.580278	8.4:-656832	MS.gene43439:CDS
ATCAAACAAAGCATCGCTTG+TGG	0.584167	8.4:+656921	None:intergenic
ATCACAAATCACAAGGCTCA+AGG	0.601072	8.4:-656340	MS.gene43439:CDS
AAGGCTCAAGGAATTGATAC+GGG	0.604072	8.4:-656328	MS.gene43439:CDS
TTCCAACTTATCATGAAACA+CGG	0.609205	8.4:+656387	None:intergenic
TTGATGTTCCTCTGGCAGCA+AGG	0.614555	8.4:+656240	None:intergenic
TAAACTACTAACCAGTCTCA+TGG	0.620534	8.4:+656191	None:intergenic
AGAAGTCATGAGTGATTACA+GGG	0.629088	8.4:-656836	MS.gene43439:CDS
ATTCAATGCCTTGCTGCCAG+AGG	0.639665	8.4:-656248	MS.gene43439:CDS
ACAAGTTATCACAAATCACA+AGG	0.640420	8.4:-656347	MS.gene43439:CDS
CTGTTGCAAGATCATAAACA+AGG	0.655865	8.4:-656454	MS.gene43439:CDS
GCTCAAGGAATTGATACGGG+AGG	0.704641	8.4:-656325	MS.gene43439:CDS
GATGGACGAATTGATCCAAG+AGG	0.740587	8.4:-656814	MS.gene43439:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTATGATTTCATAATTCTT+TGG	+	chr8.4:656411-656430	None:intergenic	20.0%
!!	ATCCCAAAATTAAATTTGTA+TGG	+	chr8.4:656863-656882	None:intergenic	20.0%
!!	TTCCCAAAATTAAATGTTTA+TGG	+	chr8.4:656374-656393	None:intergenic	20.0%
!!!	AGACCATACAAATTTAATTT+TGG	-	chr8.4:656857-656876	MS.gene43439:CDS	20.0%
!!!	GACCATACAAATTTAATTTT+GGG	-	chr8.4:656858-656877	MS.gene43439:CDS	20.0%
!!!	AGGCCATAAACATTTAATTT+TGG	-	chr8.4:656368-656387	MS.gene43439:CDS	25.0%
!!!	GGCCATAAACATTTAATTTT+GGG	-	chr8.4:656369-656388	MS.gene43439:CDS	25.0%
	ACAAGTTATCACAAATCACA+AGG	-	chr8.4:656782-656801	MS.gene43439:CDS	30.0%
	AGTTGTGTTTCAAGATAAGA+TGG	-	chr8.4:656491-656510	MS.gene43439:CDS	30.0%
	ATTGCGATGAAGCTATTTAA+AGG	-	chr8.4:656588-656607	MS.gene43439:CDS	30.0%
	TTCCAACTTATCATGAAACA+CGG	+	chr8.4:656745-656764	None:intergenic	30.0%
	TTGCGATGAAGCTATTTAAA+GGG	-	chr8.4:656589-656608	MS.gene43439:CDS	30.0%
	AATGTGAATCCGTATCTTTC+TGG	+	chr8.4:656651-656670	None:intergenic	35.0%
	AGAAGTCATGAGTGATTACA+GGG	-	chr8.4:656293-656312	MS.gene43439:CDS	35.0%
	AGAGTGCATGAGTTGTTTAA+AGG	-	chr8.4:656348-656367	MS.gene43439:CDS	35.0%
	CTGTTGCAAGATCATAAACA+AGG	-	chr8.4:656675-656694	MS.gene43439:CDS	35.0%
	TACTTCTTGCCAGAAAGATA+CGG	-	chr8.4:656639-656658	MS.gene43439:CDS	35.0%
	TGAAATCATACTTCCTCCAT+CGG	-	chr8.4:656419-656438	MS.gene43439:CDS	35.0%
	TGTTTATGATCTTGCAACAG+TGG	+	chr8.4:656675-656694	None:intergenic	35.0%
	AAGGCTCAAGGAATTGATAC+GGG	-	chr8.4:656801-656820	MS.gene43439:CDS	40.0%
	AGCCGTGTTTCATGATAAGT+TGG	-	chr8.4:656740-656759	MS.gene43439:CDS	40.0%
	AGTGTGATTTGATGTTCCTC+TGG	+	chr8.4:656900-656919	None:intergenic	40.0%
	ATCACAAATCACAAGGCTCA+AGG	-	chr8.4:656789-656808	MS.gene43439:CDS	40.0%
	CAAGGCTCAAGGAATTGATA+CGG	-	chr8.4:656800-656819	MS.gene43439:CDS	40.0%
	CAGAAGTCATGAGTGATTAC+AGG	-	chr8.4:656292-656311	MS.gene43439:CDS	40.0%
	TCATGGCAATGAAGCTCAAA+TGG	+	chr8.4:656924-656943	None:intergenic	40.0%
!	CGATTCAGTTTTTCATCCGA+TGG	+	chr8.4:656438-656457	None:intergenic	40.0%
!	GACATGCAGATTTTACTGCA+TGG	+	chr8.4:656251-656270	None:intergenic	40.0%
!	TTCAGTTTTTCATCCGATGG+AGG	+	chr8.4:656435-656454	None:intergenic	40.0%
!!	AGGGCATGTCGATTTACTTT+CGG	-	chr8.4:656608-656627	MS.gene43439:CDS	40.0%
!!	ATCAAACAAAGCATCGCTTG+TGG	+	chr8.4:656211-656230	None:intergenic	40.0%
!!	GATGCTTTGTTTGATGATGC+AGG	-	chr8.4:656216-656235	MS.gene43439:CDS	40.0%
	ACTCTTGCTTTGACACCTCT+TGG	+	chr8.4:656333-656352	None:intergenic	45.0%
	GATGGACGAATTGATCCAAG+AGG	-	chr8.4:656315-656334	MS.gene43439:CDS	45.0%
	GTCATGAGTGATTACAGGGA+TGG	-	chr8.4:656297-656316	MS.gene43439:CDS	45.0%
!!	AATTGATACGGGAGGTGTTG+CGG	-	chr8.4:656812-656831	MS.gene43439:CDS	45.0%
!!	GGGCATGTCGATTTACTTTC+GGG	-	chr8.4:656609-656628	MS.gene43439:CDS	45.0%
	ATTCAATGCCTTGCTGCCAG+AGG	-	chr8.4:656881-656900	MS.gene43439:CDS	50.0%
	GAGCTTCATTGCCATGAGAC+TGG	-	chr8.4:656927-656946	MS.gene43439:CDS	50.0%
	GCTCAAGGAATTGATACGGG+AGG	-	chr8.4:656804-656823	MS.gene43439:CDS	50.0%
	TTGATGTTCCTCTGGCAGCA+AGG	+	chr8.4:656892-656911	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	656198	656953	656198	ID=MS.gene43439
chr8.4	mRNA	656198	656953	656198	ID=MS.gene43439.t1;Parent=MS.gene43439
chr8.4	exon	656198	656953	656198	ID=MS.gene43439.t1.exon1;Parent=MS.gene43439.t1
chr8.4	CDS	656198	656953	656198	ID=cds.MS.gene43439.t1;Parent=MS.gene43439.t1

Gene Sequence

>MS.gene43439

ATGAATAGCTCCACAAGCGATGCTTTGTTTGATGATGCAGGTGCGTATGACCATGCAGTAAAATCTGCATGTCAAGAAAAGTATTCTGAATTTGCAGAAGTCATGAGTGATTACAGGGATGGACGAATTGATCCAAGAGGTGTCAAAGCAAGAGTGCATGAGTTGTTTAAAGGCCATAAACATTTAATTTTGGGAATCAACAACATCATGCCAAAGAATTATGAAATCATACTTCCTCCATCGGATGAAAAACTGAATCGACAAGATGCAACAACTTTTCTCAAACAAGTAAAAGTTGTGTTTCAAGATAAGATGGAAAAATATTATGAGTTTCTACAAGTCATACAAGACTACATGAATCTCACAATTGATATATTAGATGTCATCAAAATTGCGATGAAGCTATTTAAAGGGCATGTCGATTTACTTTCGGGATTCAACTACTTCTTGCCAGAAAGATACGGATTCACATTTCCACTGTTGCAAGATCATAAACAAGGTCATCAATTAGTGATAAAAGATGCATTTCTCAATGAAGTCAAAGCCGTGTTTCATGATAAGTTGGAAAAATATTTTGAGTTTTTACAAGTTATCACAAATCACAAGGCTCAAGGAATTGATACGGGAGGTGTTGCGGCAATAGTGAAAGAGTTATTTAAAGACCATACAAATTTAATTTTGGGATTCAATGCCTTGCTGCCAGAGGAACATCAAATCACACTTCCATTTGAGCTTCATTGCCATGAGACTGGTTAG

Protein sequence

>MS.gene43439.t1

MNSSTSDALFDDAGAYDHAVKSACQEKYSEFAEVMSDYRDGRIDPRGVKARVHELFKGHKHLILGINNIMPKNYEIILPPSDEKLNRQDATTFLKQVKVVFQDKMEKYYEFLQVIQDYMNLTIDILDVIKIAMKLFKGHVDLLSGFNYFLPERYGFTFPLLQDHKQGHQLVIKDAFLNEVKAVFHDKLEKYFEFLQVITNHKAQGIDTGGVAAIVKELFKDHTNLILGFNALLPEEHQITLPFELHCHETG