AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99643


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99643.t1 MTR_1g104960 93.642 173 11 0 1 173 1 173 1.71e-114 322
MS.gene99643.t1 MTR_1g104960 55.172 87 39 0 5 91 91 177 1.30e-24 94.4
MS.gene99643.t1 MTR_1g104960 54.023 87 40 0 89 175 3 89 5.17e-23 90.5
MS.gene99643.t1 MTR_1g104980 69.182 159 34 1 17 175 3 146 1.92e-72 216
MS.gene99643.t1 MTR_1g104980 57.895 76 32 0 17 92 74 149 1.05e-21 87.0
MS.gene99643.t1 MTR_1g104980 58.824 68 28 0 101 168 1 68 4.18e-20 82.8
MS.gene99643.t1 MTR_4g133300 49.057 159 78 1 13 171 89 244 8.37e-43 143
MS.gene99643.t1 MTR_4g133300 42.857 175 90 3 1 175 1 165 4.82e-32 115
MS.gene99643.t1 MTR_4g133470 49.375 160 76 3 13 171 89 244 5.40e-41 138
MS.gene99643.t1 MTR_4g133470 41.714 175 92 3 1 175 1 165 6.22e-30 110
MS.gene99643.t1 MTR_3g006150 47.651 149 76 2 21 168 13 160 1.78e-35 122
MS.gene99643.t1 MTR_3g006150 47.297 74 36 1 100 173 9 79 4.52e-15 69.3
MS.gene99643.t1 MTR_3g006105 48.322 149 71 4 27 170 23 170 2.07e-35 122
MS.gene99643.t1 MTR_3g006105 43.421 76 42 1 100 174 9 84 5.01e-12 61.6
MS.gene99643.t1 MTR_3g006125 48.684 152 73 3 27 173 104 255 3.14e-35 124
MS.gene99643.t1 MTR_3g006185 46.154 169 84 4 11 173 6 173 6.05e-35 120
MS.gene99643.t1 MTR_1g105955 39.759 166 93 1 2 167 5 163 2.31e-34 119
MS.gene99643.t1 MTR_1g105955 43.243 74 42 0 98 171 15 88 3.05e-15 70.1
MS.gene99643.t1 MTR_1g104937 63.441 93 34 0 6 98 5 97 3.04e-33 114
MS.gene99643.t1 MTR_1g104937 57.895 76 32 0 100 175 13 88 5.37e-24 90.9
MS.gene99643.t1 MTR_3g006065 46.104 154 79 3 23 173 15 167 5.92e-33 116
MS.gene99643.t1 MTR_1g104950 67.059 85 24 2 36 120 1 81 4.22e-32 110
MS.gene99643.t1 MTR_1g104950 61.111 54 21 0 122 175 1 54 2.97e-16 70.1
MS.gene99643.t1 MTR_5g036670 39.869 153 92 0 11 163 41 193 2.13e-31 119
MS.gene99643.t1 MTR_5g036670 50.000 76 37 1 100 175 44 118 4.90e-18 81.3
MS.gene99643.t1 MTR_1g104940 65.000 80 28 0 13 92 14 93 3.77e-30 107
MS.gene99643.t1 MTR_1g104940 55.696 79 35 0 97 175 12 90 1.34e-25 95.5
MS.gene99643.t1 MTR_8g062700 38.816 152 93 0 11 162 40 191 3.79e-26 104
MS.gene99643.t1 MTR_8g062700 51.316 76 36 1 100 175 43 117 1.11e-18 82.8
MS.gene99643.t1 MTR_8g062700 38.816 152 93 0 11 162 40 191 3.86e-26 104
MS.gene99643.t1 MTR_8g062700 51.316 76 36 1 100 175 43 117 1.11e-18 82.8
MS.gene99643.t1 MTR_6g008995 37.748 151 92 2 13 163 37 185 3.27e-25 100
MS.gene99643.t1 MTR_6g008995 44.444 72 40 0 100 171 38 109 4.01e-15 72.4
MS.gene99643.t1 MTR_7g013380 33.526 173 109 3 1 169 1 171 1.93e-24 97.1
MS.gene99643.t1 MTR_7g013380 34.940 166 102 3 13 174 99 262 5.77e-24 95.5
MS.gene99643.t1 MTR_6g008970 35.948 153 98 0 11 163 61 213 1.53e-23 97.1
MS.gene99643.t1 MTR_6g008970 44.000 75 42 0 100 174 64 138 6.66e-15 72.0
MS.gene99643.t1 MTR_1g105960 48.684 76 39 0 100 175 2 77 3.03e-20 81.3
MS.gene99643.t1 MTR_1g105960 37.190 121 50 1 14 134 2 96 3.13e-17 73.6
MS.gene99643.t1 MTR_6g008995 36.434 129 80 2 35 163 2 128 2.48e-18 81.6
MS.gene99643.t1 MTR_1g109550 38.806 134 68 4 3 136 10 129 1.64e-17 75.1
MS.gene99643.t1 MTR_1g109550 44.615 65 35 1 102 166 19 82 7.12e-12 60.5
MS.gene99643.t1 MTR_1g105010 51.429 70 34 0 98 167 15 84 3.29e-17 73.6
MS.gene99643.t1 MTR_1g105010 49.231 65 33 0 11 75 14 78 8.26e-13 62.4
MS.gene99643.t1 MTR_2g097830 41.525 118 59 3 1 118 59 166 7.69e-16 71.2
MS.gene99643.t1 MTR_2g097830 35.659 129 71 2 50 169 12 137 8.96e-13 63.2
MS.gene99643.t1 MTR_7g013410 26.027 146 103 1 16 161 1 141 1.66e-15 69.7
MS.gene99643.t1 MTR_7g013410 44.776 67 37 0 103 169 2 68 1.47e-11 59.3
MS.gene99643.t1 MTR_8g062350 42.500 80 46 0 89 168 4 83 1.12e-14 68.9
MS.gene99643.t1 MTR_8g062350 40.659 91 52 1 12 102 13 101 1.63e-14 68.6
MS.gene99643.t1 MTR_3g006010 55.172 58 26 0 27 84 23 80 9.70e-13 65.5
MS.gene99643.t1 MTR_1g093310 41.026 78 38 2 94 170 4 74 2.47e-12 60.1
MS.gene99643.t1 MTR_7g089490 39.189 74 44 1 90 163 8 80 9.05e-12 59.7
MS.gene99643.t1 MTR_7g089490 47.761 67 35 0 11 77 14 80 1.45e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99643.t1 AT5G15020 37.662 154 92 2 11 163 50 200 4.92e-25 101
MS.gene99643.t1 AT5G15020 42.105 76 44 0 93 168 46 121 6.08e-14 69.3
MS.gene99643.t1 AT5G15020 37.662 154 92 2 11 163 50 200 4.96e-25 101
MS.gene99643.t1 AT5G15020 42.105 76 44 0 93 168 46 121 5.90e-14 69.3
MS.gene99643.t1 AT3G01320 36.905 168 99 3 4 169 46 208 4.88e-24 98.6
MS.gene99643.t1 AT3G01320 36.905 168 99 3 4 169 46 208 5.43e-24 98.2
MS.gene99643.t1 AT1G24230 34.146 164 92 3 11 173 12 160 1.60e-23 93.6
MS.gene99643.t1 AT1G70060 37.908 153 94 1 11 162 12 164 1.76e-23 96.7
MS.gene99643.t1 AT1G70060 47.826 69 36 0 100 168 15 83 7.29e-15 72.0
MS.gene99643.t1 AT1G70060 37.908 153 94 1 11 162 12 164 1.76e-23 96.7
MS.gene99643.t1 AT1G70060 47.826 69 36 0 100 168 15 83 7.29e-15 72.0
MS.gene99643.t1 AT1G24190 36.184 152 97 0 11 162 12 163 4.35e-23 95.5
MS.gene99643.t1 AT1G24190 45.946 74 39 1 100 172 15 88 5.57e-15 72.4
MS.gene99643.t1 AT1G24190 36.184 152 97 0 11 162 12 163 4.61e-23 95.5
MS.gene99643.t1 AT1G24190 45.946 74 39 1 100 172 15 88 5.73e-15 72.4
MS.gene99643.t1 AT1G24190 36.184 152 97 0 11 162 12 163 4.79e-23 95.5
MS.gene99643.t1 AT1G24190 45.946 74 39 1 100 172 15 88 5.68e-15 72.4
MS.gene99643.t1 AT1G27260 37.419 155 91 4 11 160 1 154 5.36e-23 91.7
MS.gene99643.t1 AT1G27260 42.667 75 43 0 98 172 2 76 3.84e-12 62.8
MS.gene99643.t1 AT1G27260 32.203 118 79 1 11 128 91 207 6.27e-11 59.3
MS.gene99643.t1 AT1G10450 35.948 153 96 2 9 161 80 230 5.97e-22 92.4
MS.gene99643.t1 AT1G10450 47.826 69 36 0 100 168 85 153 3.57e-14 70.1
MS.gene99643.t1 AT1G10450 35.948 153 96 2 9 161 29 179 7.86e-22 92.0
MS.gene99643.t1 AT1G10450 47.826 69 36 0 100 168 34 102 3.69e-14 70.1
MS.gene99643.t1 AT1G10450 35.948 153 96 2 9 161 29 179 7.86e-22 92.0
MS.gene99643.t1 AT1G10450 47.826 69 36 0 100 168 34 102 3.69e-14 70.1
MS.gene99643.t1 AT1G24200 36.601 153 94 2 9 161 10 159 8.28e-22 87.8
MS.gene99643.t1 AT1G24200 45.588 68 37 0 100 167 15 82 2.37e-14 68.2
MS.gene99643.t1 AT1G24250 30.994 171 96 3 11 160 12 181 1.62e-21 88.2
MS.gene99643.t1 AT1G24250 43.478 69 39 0 6 74 113 181 9.48e-11 59.3
MS.gene99643.t1 AT1G23810 30.178 169 107 4 2 160 3 170 3.29e-20 84.7
MS.gene99643.t1 AT1G70030 36.242 149 93 2 13 161 11 157 3.40e-20 82.8
MS.gene99643.t1 AT1G70030 43.056 72 41 0 100 171 12 83 5.82e-11 58.5
MS.gene99643.t1 AT1G59890 34.641 153 92 2 13 162 45 192 2.71e-19 84.7
MS.gene99643.t1 AT1G59890 46.377 69 37 0 100 168 46 114 3.54e-14 70.1
MS.gene99643.t1 AT1G59890 34.641 153 92 2 13 162 45 192 2.76e-19 84.7
MS.gene99643.t1 AT1G59890 46.377 69 37 0 100 168 46 114 3.37e-14 70.1
MS.gene99643.t1 AT1G59890 34.641 153 92 2 13 162 45 192 3.06e-19 84.7
MS.gene99643.t1 AT1G59890 46.377 69 37 0 100 168 46 114 3.74e-14 70.1
MS.gene99643.t1 AT1G59890 34.641 153 92 2 13 162 45 192 3.16e-19 84.7
MS.gene99643.t1 AT1G59890 46.377 69 37 0 100 168 46 114 3.64e-14 70.1
MS.gene99643.t1 AT1G27280 33.566 143 93 1 21 161 1 143 5.33e-17 75.9
MS.gene99643.t1 AT1G27220 45.070 71 39 0 11 81 12 82 8.69e-16 71.6
MS.gene99643.t1 AT1G27220 36.986 73 46 0 95 167 10 82 3.02e-11 59.7
MS.gene99643.t1 AT5G15040 46.269 67 36 0 102 168 6 72 1.89e-14 65.9
MS.gene99643.t1 AT5G15040 42.857 77 42 1 13 89 3 77 1.87e-13 63.2
MS.gene99643.t1 AT5G15040 46.269 67 36 0 102 168 6 72 1.89e-14 65.9
MS.gene99643.t1 AT5G15040 42.857 77 42 1 13 89 3 77 1.87e-13 63.2
MS.gene99643.t1 AT5G15040 46.269 67 36 0 102 168 6 72 1.89e-14 65.9
MS.gene99643.t1 AT5G15040 42.857 77 42 1 13 89 3 77 1.87e-13 63.2
MS.gene99643.t1 AT1G27250 47.826 69 36 0 12 80 20 88 1.17e-13 65.1
MS.gene99643.t1 AT5G15025 41.975 81 47 0 2 82 36 116 2.16e-13 63.9
MS.gene99643.t1 AT5G15025 40.845 71 42 0 98 168 46 116 1.27e-12 62.0
MS.gene99643.t1 AT1G70030 38.261 115 65 2 13 122 22 135 2.61e-13 63.9
MS.gene99643.t1 AT1G70030 43.056 72 41 0 100 171 23 94 5.26e-11 58.2
MS.gene99643.t1 AT1G70030 40.206 97 57 1 13 109 22 117 9.01e-13 62.4
MS.gene99643.t1 AT1G70030 43.056 72 41 0 100 171 23 94 2.54e-11 58.5
MS.gene99643.t1 AT1G27270 32.716 162 96 2 12 160 13 174 9.93e-13 64.3
MS.gene99643.t1 AT1G24210 42.667 75 41 1 4 76 3 77 1.31e-12 62.8
MS.gene99643.t1 AT5G35610 40.230 87 49 1 1 87 1 84 4.03e-11 58.5

Find 30 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAACTCCTACAAGATTT+TGG 0.194654 1.4:+80781655 MS.gene99643:CDS
AACTCTTACATCAATTCTTT+TGG 0.285232 1.4:-80781938 None:intergenic
GACTCTTCTATCAATTCTTT+TGG 0.310378 1.4:-80781680 None:intergenic
GGTTACATTTGAAGATAAGA+TGG 0.406022 1.4:+80781873 MS.gene99643:CDS
AAGCTGTGTTTCAAGATAAG+AGG 0.447897 1.4:+80781614 MS.gene99643:CDS
CACCCTTGCATCGTCAATCA+AGG 0.459246 1.4:-80781575 None:intergenic
ATGAACAGCTCAAGAAGAAC+AGG 0.465556 1.4:+80781550 MS.gene99643:CDS
AGTCGTCAAAGAAGGAGTTA+TGG 0.477577 1.4:+80781699 MS.gene99643:CDS
AGTTGTCAAAGAAGGAATGA+TGG 0.479247 1.4:+80781957 MS.gene99643:CDS
ACATGAAATCCCACTTCCAT+TGG 0.486277 1.4:+80781780 MS.gene99643:CDS
GATAGAAGAGTCGTCAAAGA+AGG 0.515587 1.4:+80781691 MS.gene99643:CDS
AAATCATCATCCAATGGAAG+TGG 0.515740 1.4:-80781790 None:intergenic
TTCAACATCTTCTTGCCACC+AGG 0.516818 1.4:+80781757 MS.gene99643:CDS
ACACAAAATCTCACTTCCAT+TGG 0.521284 1.4:+80782038 MS.gene99643:CDS
GTCCCTTGATTGACGATGCA+AGG 0.537025 1.4:+80781572 MS.gene99643:CDS
AGCTGTGTTTCAAGATAAGA+GGG 0.537554 1.4:+80781615 MS.gene99643:CDS
GATGTAAGAGTTGTCAAAGA+AGG 0.540360 1.4:+80781949 MS.gene99643:CDS
CGATGCAAGGGTGTTTGTAA+AGG 0.542038 1.4:+80781585 MS.gene99643:CDS
TTTGTGTCCCTCTGGCAAGA+AGG 0.563533 1.4:-80782022 None:intergenic
TATTGTTCATCACCATCCAA+TGG 0.573399 1.4:-80782054 None:intergenic
GGAAGTGGGATTTCATGTCC+TGG 0.588837 1.4:-80781775 None:intergenic
TTCAACACCTTCTTGCCAGA+GGG 0.592249 1.4:+80782015 MS.gene99643:CDS
AAAATCTCACTTCCATTGGA+TGG 0.592611 1.4:+80782042 MS.gene99643:CDS
TCCCTTGATTGACGATGCAA+GGG 0.593982 1.4:+80781573 MS.gene99643:CDS
TGTTGTAAATCATCATCCAA+TGG 0.600709 1.4:-80781796 None:intergenic
AATCATCATCCAATGGAAGT+GGG 0.616288 1.4:-80781789 None:intergenic
ACCCTTGCATCGTCAATCAA+GGG 0.616892 1.4:-80781574 None:intergenic
ATTCAACACCTTCTTGCCAG+AGG 0.655191 1.4:+80782014 MS.gene99643:CDS
AGCGCTCGAAGATCGAGCAA+TGG 0.663840 1.4:+80781831 MS.gene99643:CDS
AGTGGGATTTCATGTCCTGG+TGG 0.734772 1.4:-80781772 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGACCATAAAAATTTACTTT+TGG + chr1.4:80781990-80782009 MS.gene99643:CDS 20.0%
!!! ATCCCAAAAGTAAATTTTTA+TGG - chr1.4:80781996-80782015 None:intergenic 20.0%
!!! GACCATAAAAATTTACTTTT+GGG + chr1.4:80781991-80782010 MS.gene99643:CDS 20.0%
!! AACTCTTACATCAATTCTTT+TGG - chr1.4:80781941-80781960 None:intergenic 25.0%
GGTTACATTTGAAGATAAGA+TGG + chr1.4:80781873-80781892 MS.gene99643:CDS 30.0%
TGTTGTAAATCATCATCCAA+TGG - chr1.4:80781799-80781818 None:intergenic 30.0%
! GACTCTTCTATCAATTCTTT+TGG - chr1.4:80781683-80781702 None:intergenic 30.0%
! TTACATCAATTCTTTTGGCA+CGG - chr1.4:80781936-80781955 None:intergenic 30.0%
AAAATCTCACTTCCATTGGA+TGG + chr1.4:80782042-80782061 MS.gene99643:CDS 35.0%
AAATCATCATCCAATGGAAG+TGG - chr1.4:80781793-80781812 None:intergenic 35.0%
AAGCTGTGTTTCAAGATAAG+AGG + chr1.4:80781614-80781633 MS.gene99643:CDS 35.0%
AATCATCATCCAATGGAAGT+GGG - chr1.4:80781792-80781811 None:intergenic 35.0%
ACACAAAATCTCACTTCCAT+TGG + chr1.4:80782038-80782057 MS.gene99643:CDS 35.0%
AGCTGTGTTTCAAGATAAGA+GGG + chr1.4:80781615-80781634 MS.gene99643:CDS 35.0%
AGTTGTCAAAGAAGGAATGA+TGG + chr1.4:80781957-80781976 MS.gene99643:CDS 35.0%
GATGTAAGAGTTGTCAAAGA+AGG + chr1.4:80781949-80781968 MS.gene99643:CDS 35.0%
TATTGTTCATCACCATCCAA+TGG - chr1.4:80782057-80782076 None:intergenic 35.0%
! GGCGTTTTTAACAACAGTTA+AGG + chr1.4:80781852-80781871 MS.gene99643:CDS 35.0%
! GTGAAACTCCTACAAGATTT+TGG + chr1.4:80781655-80781674 MS.gene99643:CDS 35.0%
ACATGAAATCCCACTTCCAT+TGG + chr1.4:80781780-80781799 MS.gene99643:CDS 40.0%
AGTCGTCAAAGAAGGAGTTA+TGG + chr1.4:80781699-80781718 MS.gene99643:CDS 40.0%
ATGAACAGCTCAAGAAGAAC+AGG + chr1.4:80781550-80781569 MS.gene99643:CDS 40.0%
GATAGAAGAGTCGTCAAAGA+AGG + chr1.4:80781691-80781710 MS.gene99643:CDS 40.0%
! TTTTGGCGCCAAAATCTTGT+AGG - chr1.4:80781666-80781685 None:intergenic 40.0%
ACCCTTGCATCGTCAATCAA+GGG - chr1.4:80781577-80781596 None:intergenic 45.0%
ATTCAACACCTTCTTGCCAG+AGG + chr1.4:80782014-80782033 MS.gene99643:CDS 45.0%
TCCCTTGATTGACGATGCAA+GGG + chr1.4:80781573-80781592 MS.gene99643:CDS 45.0%
TTCAACACCTTCTTGCCAGA+GGG + chr1.4:80782015-80782034 MS.gene99643:CDS 45.0%
TTCAACATCTTCTTGCCACC+AGG + chr1.4:80781757-80781776 MS.gene99643:CDS 45.0%
! AGTGAGATTTTGTGTCCCTC+TGG - chr1.4:80782033-80782052 None:intergenic 45.0%
! TTTTGGCACGGTAATCAGCT+AGG - chr1.4:80781924-80781943 None:intergenic 45.0%
!! CGATGCAAGGGTGTTTGTAA+AGG + chr1.4:80781585-80781604 MS.gene99643:CDS 45.0%
AGTGGGATTTCATGTCCTGG+TGG - chr1.4:80781775-80781794 None:intergenic 50.0%
CACCCTTGCATCGTCAATCA+AGG - chr1.4:80781578-80781597 None:intergenic 50.0%
GGAAGTGGGATTTCATGTCC+TGG - chr1.4:80781778-80781797 None:intergenic 50.0%
GTCCCTTGATTGACGATGCA+AGG + chr1.4:80781572-80781591 MS.gene99643:CDS 50.0%
TTTGTGTCCCTCTGGCAAGA+AGG - chr1.4:80782025-80782044 None:intergenic 50.0%
AGCGCTCGAAGATCGAGCAA+TGG + chr1.4:80781831-80781850 MS.gene99643:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 80781550 80782077 80781550 ID=MS.gene99643
chr1.4 mRNA 80781550 80782077 80781550 ID=MS.gene99643.t1;Parent=MS.gene99643
chr1.4 exon 80781550 80782077 80781550 ID=MS.gene99643.t1.exon1;Parent=MS.gene99643.t1
chr1.4 CDS 80781550 80782077 80781550 ID=cds.MS.gene99643.t1;Parent=MS.gene99643.t1
Gene Sequence

>MS.gene99643

ATGAACAGCTCAAGAAGAACAGGTCCCTTGATTGACGATGCAAGGGTGTTTGTAAAGGATGTAGAAGCTGTGTTTCAAGATAAGAGGGAGAAGTATGATGAATTCGTGAAACTCCTACAAGATTTTGGCGCCAAAAGAATTGATAGAAGAGTCGTCAAAGAAGGAGTTATGGAGTTGTTGAAAGAGCATCAAGATTTAATTTCAAGATTCAACATCTTCTTGCCACCAGGACATGAAATCCCACTTCCATTGGATGATGATTTACAACAACGTGATCAATCAGCGCTCGAAGATCGAGCAATGGCGTTTTTAACAACAGTTAAGGTTACATTTGAAGATAAGATGGAAAAGTTCAATGAATTCTTGAAACTCCTAGCTGATTACCGTGCCAAAAGAATTGATGTAAGAGTTGTCAAAGAAGGAATGATGGAGTTGTTTAAAGACCATAAAAATTTACTTTTGGGATTCAACACCTTCTTGCCAGAGGGACACAAAATCTCACTTCCATTGGATGGTGATGAACAATAA

Protein sequence

>MS.gene99643.t1

MNSSRRTGPLIDDARVFVKDVEAVFQDKREKYDEFVKLLQDFGAKRIDRRVVKEGVMELLKEHQDLISRFNIFLPPGHEIPLPLDDDLQQRDQSALEDRAMAFLTTVKVTFEDKMEKFNEFLKLLADYRAKRIDVRVVKEGMMELFKDHKNLLLGFNTFLPEGHKISLPLDGDEQ