AlfalfaGEDB | MS.gene80088

M. sativa cultivar XinJiangDaYe / MS.gene80088

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24 PF16545.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80088.t1	XP_003592441.1	87	177	23	0	1	177	1	177	2.40E-80	308.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80088.t1	O04539	40.1	152	90	1	12	162	13	164	1.0e-23	112.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80088.t1	G7IE22	87.0	177	23	0	1	177	1	177	1.7e-80	308.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene80088.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene60770	MS.gene80088	PPI
MS.gene99644	MS.gene80088	PPI
MS.gene80089	MS.gene80088	PPI
MS.gene80088	MS.gene60770	PPI
MS.gene80088	MS.gene99644	PPI
MS.gene80088	MS.gene80087	PPI
MS.gene80088	MS.gene71600	PPI
MS.gene80087	MS.gene80088	PPI
MS.gene80088	MS.gene99643	PPI
MS.gene80088	MS.gene43439	PPI
MS.gene80088	MS.gene012752	PPI
MS.gene80088	MS.gene91422	PPI
MS.gene80088	MS.gene80089	PPI
MS.gene91421	MS.gene80088	PPI
MS.gene80088	MS.gene93864	PPI
MS.gene91330	MS.gene80088	PPI
MS.gene80088	MS.gene91421	PPI
MS.gene80088	MS.gene93863	PPI
MS.gene80088	MS.gene93862	PPI
MS.gene85574	MS.gene80088	PPI
MS.gene80088	MS.gene95089	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80088.t1	MTR_1g104960	87.006	177	23	0	1	177	1	177	1.76e-107	309
MS.gene80088.t1	MTR_1g104960	72.289	166	46	0	94	259	8	173	3.31e-79	237
MS.gene80088.t1	MTR_1g104960	52.577	97	45	1	175	271	3	98	1.28e-23	94.7
MS.gene80088.t1	MTR_1g104980	64.198	162	43	1	17	178	3	149	7.95e-65	201
MS.gene80088.t1	MTR_1g104980	55.497	191	57	2	101	278	1	176	1.23e-61	192
MS.gene80088.t1	MTR_1g104980	62.338	77	29	0	16	92	73	149	2.15e-23	94.4
MS.gene80088.t1	MTR_4g133300	43.580	257	132	4	1	257	1	244	4.17e-58	186
MS.gene80088.t1	MTR_4g133300	42.515	167	87	2	96	262	9	166	4.34e-33	121
MS.gene80088.t1	MTR_4g133470	42.802	257	134	4	1	257	1	244	1.48e-55	179
MS.gene80088.t1	MTR_4g133470	42.857	168	85	4	96	262	9	166	3.28e-31	117
MS.gene80088.t1	MTR_1g105955	38.235	170	98	1	84	253	1	163	4.41e-36	127
MS.gene80088.t1	MTR_1g105955	40.964	166	91	1	2	167	5	163	1.82e-34	123
MS.gene80088.t1	MTR_7g013380	31.203	266	173	5	1	260	1	262	1.12e-35	129
MS.gene80088.t1	MTR_3g006150	48.276	145	73	2	21	164	13	156	5.70e-34	121
MS.gene80088.t1	MTR_3g006150	42.675	157	85	3	99	254	8	160	1.44e-31	115
MS.gene80088.t1	MTR_3g006150	43.182	88	46	2	185	272	8	91	3.24e-13	66.2
MS.gene80088.t1	MTR_3g006125	43.195	169	90	3	11	173	87	255	5.04e-32	119
MS.gene80088.t1	MTR_3g006125	39.521	167	95	3	99	259	89	255	3.50e-27	106
MS.gene80088.t1	MTR_1g104950	68.235	85	23	2	36	120	1	81	5.64e-32	113
MS.gene80088.t1	MTR_1g104950	55.294	85	34	2	122	206	1	81	6.82e-22	87.4
MS.gene80088.t1	MTR_1g104950	50.000	70	25	1	208	277	1	60	1.25e-14	68.2
MS.gene80088.t1	MTR_1g104937	61.702	94	36	0	6	99	5	98	6.41e-32	114
MS.gene80088.t1	MTR_1g104937	56.322	87	38	0	99	185	12	98	3.55e-25	97.1
MS.gene80088.t1	MTR_1g104937	51.111	90	43	1	185	274	12	100	9.43e-22	87.8
MS.gene80088.t1	MTR_3g006185	42.941	170	88	4	11	173	6	173	7.27e-32	116
MS.gene80088.t1	MTR_3g006185	39.157	166	96	3	99	259	8	173	2.05e-26	102
MS.gene80088.t1	MTR_3g006065	46.104	154	79	3	23	173	15	167	1.28e-31	116
MS.gene80088.t1	MTR_3g006065	39.759	166	93	4	99	261	8	169	4.33e-28	107
MS.gene80088.t1	MTR_5g036670	40.260	154	92	0	10	163	40	193	3.87e-31	122
MS.gene80088.t1	MTR_5g036670	36.842	152	96	0	98	249	42	193	2.00e-29	117
MS.gene80088.t1	MTR_5g036670	41.573	89	52	0	184	272	42	130	4.18e-15	75.9
MS.gene80088.t1	MTR_3g006105	42.771	166	88	4	11	170	6	170	6.09e-31	114
MS.gene80088.t1	MTR_3g006105	40.854	164	90	4	99	256	8	170	3.87e-28	107
MS.gene80088.t1	MTR_1g104940	57.000	100	42	1	1	99	1	100	4.71e-30	110
MS.gene80088.t1	MTR_1g104940	52.809	89	42	0	97	185	12	100	4.30e-26	99.8
MS.gene80088.t1	MTR_1g104940	52.632	95	44	1	183	277	12	105	1.63e-25	98.2
MS.gene80088.t1	MTR_8g062700	40.909	154	89	2	10	162	39	191	1.04e-27	112
MS.gene80088.t1	MTR_8g062700	37.086	151	95	0	98	248	41	191	4.03e-26	108
MS.gene80088.t1	MTR_8g062700	46.154	78	41	1	184	261	41	117	7.87e-15	75.1
MS.gene80088.t1	MTR_8g062700	40.909	154	89	2	10	162	39	191	1.05e-27	112
MS.gene80088.t1	MTR_8g062700	37.086	151	95	0	98	248	41	191	4.15e-26	108
MS.gene80088.t1	MTR_8g062700	46.154	78	41	1	184	261	41	117	7.94e-15	74.7
MS.gene80088.t1	MTR_6g008995	37.748	151	92	2	13	163	37	185	2.06e-24	102
MS.gene80088.t1	MTR_6g008995	35.762	151	95	2	99	249	37	185	3.60e-24	101
MS.gene80088.t1	MTR_6g008970	36.364	154	98	0	10	163	60	213	9.12e-24	101
MS.gene80088.t1	MTR_6g008970	33.553	152	101	0	98	249	62	213	3.68e-22	96.7
MS.gene80088.t1	MTR_6g008970	37.079	89	56	0	184	272	62	150	1.53e-11	65.1
MS.gene80088.t1	MTR_1g109550	40.625	128	66	3	7	134	10	127	3.53e-20	84.7
MS.gene80088.t1	MTR_1g109550	36.842	133	74	3	88	220	5	127	1.63e-17	77.8
MS.gene80088.t1	MTR_1g109550	34.483	87	52	2	166	252	1	82	5.23e-11	59.7
MS.gene80088.t1	MTR_1g105960	47.500	80	42	0	186	265	2	81	2.92e-19	81.3
MS.gene80088.t1	MTR_1g105960	48.649	74	38	0	100	173	2	75	9.66e-19	80.1
MS.gene80088.t1	MTR_1g105960	37.190	121	50	1	14	134	2	96	7.90e-17	74.7
MS.gene80088.t1	MTR_6g008995	34.884	129	82	2	121	249	2	128	1.80e-18	84.7
MS.gene80088.t1	MTR_6g008995	36.434	129	80	2	35	163	2	128	2.40e-17	81.6
MS.gene80088.t1	MTR_2g097830	36.429	140	71	3	74	204	36	166	7.16e-17	76.6
MS.gene80088.t1	MTR_2g097830	42.017	119	57	4	1	118	59	166	2.76e-15	72.4
MS.gene80088.t1	MTR_7g013410	28.082	146	100	1	102	247	1	141	3.76e-16	73.9
MS.gene80088.t1	MTR_7g013410	28.276	145	99	1	17	161	2	141	1.12e-15	72.8
MS.gene80088.t1	MTR_1g105010	50.000	70	35	0	98	167	15	84	1.89e-15	71.2
MS.gene80088.t1	MTR_1g105010	42.353	85	46	1	184	268	15	96	3.49e-14	67.8
MS.gene80088.t1	MTR_1g105010	48.611	72	37	0	10	81	13	84	6.63e-14	67.0
MS.gene80088.t1	MTR_8g062350	42.045	88	49	1	97	184	12	97	1.07e-14	71.6
MS.gene80088.t1	MTR_8g062350	41.758	91	51	1	12	102	13	101	1.05e-13	68.6
MS.gene80088.t1	MTR_8g062350	39.773	88	51	1	175	262	4	89	3.37e-13	67.4
MS.gene80088.t1	MTR_7g089490	47.761	67	35	0	11	77	14	80	1.83e-11	60.5
MS.gene80088.t1	MTR_3g006010	51.724	58	28	0	27	84	23	80	4.30e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80088.t1	AT1G24230	30.400	250	146	7	10	251	11	240	6.96e-27	105
MS.gene80088.t1	AT1G24230	36.196	163	88	2	98	259	13	160	5.14e-25	100
MS.gene80088.t1	AT1G70060	39.610	154	92	1	10	162	11	164	1.87e-25	106
MS.gene80088.t1	AT1G70060	35.526	152	97	1	98	248	13	164	4.03e-23	99.4
MS.gene80088.t1	AT1G70060	45.070	71	39	0	184	254	13	83	1.31e-11	65.5
MS.gene80088.t1	AT1G70060	39.610	154	92	1	10	162	11	164	1.87e-25	106
MS.gene80088.t1	AT1G70060	35.526	152	97	1	98	248	13	164	4.03e-23	99.4
MS.gene80088.t1	AT1G70060	45.070	71	39	0	184	254	13	83	1.31e-11	65.5
MS.gene80088.t1	AT1G27260	38.333	180	103	3	98	272	2	178	3.26e-25	100
MS.gene80088.t1	AT1G27260	35.577	208	129	3	11	214	1	207	2.24e-24	98.2
MS.gene80088.t1	AT5G15020	38.065	155	92	2	10	163	49	200	4.73e-25	105
MS.gene80088.t1	AT5G15020	35.294	153	95	2	98	249	51	200	8.83e-24	101
MS.gene80088.t1	AT5G15020	40.845	71	42	0	184	254	51	121	8.27e-11	62.8
MS.gene80088.t1	AT5G15020	38.065	155	92	2	10	163	49	200	4.85e-25	105
MS.gene80088.t1	AT5G15020	35.294	153	95	2	98	249	51	200	8.49e-24	101
MS.gene80088.t1	AT5G15020	40.845	71	42	0	184	254	51	121	7.89e-11	62.8
MS.gene80088.t1	AT3G01320	36.875	160	98	2	4	161	46	204	9.17e-24	101
MS.gene80088.t1	AT3G01320	35.443	158	97	2	98	255	56	208	3.76e-23	99.4
MS.gene80088.t1	AT1G24200	35.714	154	94	2	95	247	10	159	9.68e-24	95.9
MS.gene80088.t1	AT1G24200	37.013	154	92	2	9	161	10	159	2.74e-22	92.0
MS.gene80088.t1	AT1G24200	41.429	70	41	0	184	253	13	82	2.12e-11	62.0
MS.gene80088.t1	AT3G01320	36.875	160	98	2	4	161	46	204	9.72e-24	101
MS.gene80088.t1	AT3G01320	35.443	158	97	2	98	255	56	208	3.94e-23	99.4
MS.gene80088.t1	AT1G24190	37.349	166	93	3	10	169	11	171	1.36e-23	100
MS.gene80088.t1	AT1G24190	33.113	151	101	0	98	248	13	163	2.19e-22	97.4
MS.gene80088.t1	AT1G24190	43.421	76	42	1	184	258	13	88	7.49e-12	65.9
MS.gene80088.t1	AT1G24190	37.349	166	93	3	10	169	11	171	1.52e-23	100
MS.gene80088.t1	AT1G24190	33.113	151	101	0	98	248	13	163	2.43e-22	97.1
MS.gene80088.t1	AT1G24190	43.421	76	42	1	184	258	13	88	7.99e-12	65.9
MS.gene80088.t1	AT1G24190	37.349	166	93	3	10	169	11	171	1.54e-23	100
MS.gene80088.t1	AT1G24190	32.704	159	106	1	98	255	13	171	2.36e-22	97.1
MS.gene80088.t1	AT1G24190	43.421	76	42	1	184	258	13	88	7.77e-12	65.9
MS.gene80088.t1	AT1G24250	30.317	221	133	4	11	211	12	231	3.12e-23	95.9
MS.gene80088.t1	AT1G24250	33.166	199	110	6	94	272	10	205	1.31e-20	88.6
MS.gene80088.t1	AT1G24250	46.377	69	37	0	6	74	113	181	6.33e-11	61.6
MS.gene80088.t1	AT1G10450	37.908	153	93	2	95	247	29	179	7.15e-23	98.6
MS.gene80088.t1	AT1G10450	34.031	191	109	4	9	184	29	217	1.18e-21	95.1
MS.gene80088.t1	AT1G10450	44.286	70	39	0	185	254	33	102	4.92e-11	63.5
MS.gene80088.t1	AT1G10450	37.908	153	93	2	95	247	29	179	7.15e-23	98.6
MS.gene80088.t1	AT1G10450	34.031	191	109	4	9	184	29	217	1.18e-21	95.1
MS.gene80088.t1	AT1G10450	44.286	70	39	0	185	254	33	102	4.92e-11	63.5
MS.gene80088.t1	AT1G10450	37.908	153	93	2	95	247	80	230	7.57e-23	98.6
MS.gene80088.t1	AT1G10450	34.031	191	109	4	9	184	80	268	1.07e-21	95.1
MS.gene80088.t1	AT1G10450	44.286	70	39	0	185	254	84	153	5.39e-11	63.5
MS.gene80088.t1	AT1G23810	29.224	219	145	4	2	211	3	220	2.02e-22	93.2
MS.gene80088.t1	AT1G23810	34.043	188	112	5	94	272	10	194	5.82e-21	89.4
MS.gene80088.t1	AT1G70030	37.584	149	91	2	13	161	11	157	2.17e-21	88.6
MS.gene80088.t1	AT1G70030	35.570	149	94	2	99	247	11	157	1.96e-19	83.2
MS.gene80088.t1	AT1G59890	35.329	167	103	3	13	178	45	207	3.60e-21	93.6
MS.gene80088.t1	AT1G59890	33.987	153	93	2	99	248	45	192	5.54e-19	87.4
MS.gene80088.t1	AT1G59890	44.286	70	39	0	185	254	45	114	1.49e-11	65.1
MS.gene80088.t1	AT1G59890	35.329	167	103	3	13	178	45	207	3.99e-21	93.6
MS.gene80088.t1	AT1G59890	33.987	153	93	2	99	248	45	192	5.91e-19	87.0
MS.gene80088.t1	AT1G59890	44.286	70	39	0	185	254	45	114	1.53e-11	65.1
MS.gene80088.t1	AT1G59890	35.329	167	103	3	13	178	45	207	4.10e-21	93.6
MS.gene80088.t1	AT1G59890	33.987	153	93	2	99	248	45	192	5.90e-19	87.0
MS.gene80088.t1	AT1G59890	44.286	70	39	0	185	254	45	114	1.53e-11	65.1
MS.gene80088.t1	AT1G59890	35.329	167	103	3	13	178	45	207	4.42e-21	93.6
MS.gene80088.t1	AT1G59890	33.987	153	93	2	99	248	45	192	6.55e-19	87.0
MS.gene80088.t1	AT1G59890	44.286	70	39	0	185	254	45	114	1.63e-11	65.1
MS.gene80088.t1	AT1G27280	32.000	200	127	5	21	214	1	197	2.36e-18	82.0
MS.gene80088.t1	AT1G27280	32.857	140	92	1	110	247	4	143	5.04e-13	67.4
MS.gene80088.t1	AT1G27270	34.568	162	93	2	98	246	13	174	1.75e-15	74.3
MS.gene80088.t1	AT1G27270	35.802	162	91	3	12	160	13	174	3.06e-15	73.9
MS.gene80088.t1	AT1G27220	33.945	109	65	1	9	117	10	111	7.41e-15	71.6
MS.gene80088.t1	AT1G70030	37.288	118	71	2	99	214	22	138	2.37e-14	68.9
MS.gene80088.t1	AT1G70030	40.000	115	63	2	13	122	22	135	2.68e-14	68.9
MS.gene80088.t1	AT1G70030	42.268	97	55	1	13	109	22	117	6.22e-14	67.4
MS.gene80088.t1	AT1G70030	40.206	97	57	1	99	195	22	117	1.16e-13	66.6
MS.gene80088.t1	AT5G15040	44.156	77	41	1	99	175	3	77	6.76e-14	66.2
MS.gene80088.t1	AT5G15040	42.857	77	42	1	13	89	3	77	2.10e-13	65.1
MS.gene80088.t1	AT5G15040	42.857	70	40	0	185	254	3	72	5.34e-12	61.2
MS.gene80088.t1	AT5G15040	44.156	77	41	1	99	175	3	77	6.76e-14	66.2
MS.gene80088.t1	AT5G15040	42.857	77	42	1	13	89	3	77	2.10e-13	65.1
MS.gene80088.t1	AT5G15040	42.857	70	40	0	185	254	3	72	5.34e-12	61.2
MS.gene80088.t1	AT5G15040	44.156	77	41	1	99	175	3	77	6.76e-14	66.2
MS.gene80088.t1	AT5G15040	42.857	77	42	1	13	89	3	77	2.10e-13	65.1
MS.gene80088.t1	AT5G15040	42.857	70	40	0	185	254	3	72	5.34e-12	61.2
MS.gene80088.t1	AT1G24210	45.333	75	39	1	4	76	3	77	3.67e-13	66.2
MS.gene80088.t1	AT5G15025	40.741	81	48	0	2	82	36	116	5.79e-13	64.7
MS.gene80088.t1	AT1G27250	44.928	69	38	0	12	80	20	88	5.63e-12	62.4

Find 45 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGACCATACAAATTTACTTT+TGG	0.266924	1.1:+75192924	MS.gene80088:CDS
AACTCTTACATCAAGTCTTT+TGG	0.279157	1.1:-75192872	None:intergenic
AACTCTTACATCAATTCTTT+TGG	0.285232	1.1:-75192614	None:intergenic
GACTCTTCTATCAATTCTTT+TGG	0.310378	1.1:-75192356	None:intergenic
GTGAAACTCCTACAAGATTT+CGG	0.391833	1.1:+75192331	MS.gene80088:CDS
GGTTACATTTGAAGATAAGA+TGG	0.406022	1.1:+75192549	MS.gene80088:CDS
GGTTACATTTAAAGATAAGA+TGG	0.406022	1.1:+75192807	MS.gene80088:CDS
ATCCCAAAAGTAAATTTGTA+TGG	0.424928	1.1:-75192927	None:intergenic
AGTTATGAAAGAAGGAATGA+TGG	0.450472	1.1:+75192891	MS.gene80088:CDS
CGATGCAAGGGCGTTTGTAA+AGG	0.450885	1.1:+75192261	MS.gene80088:CDS
AAGCTGTGTTTCAAGATAAG+AGG	0.458098	1.1:+75192290	MS.gene80088:CDS
ATGAACAGCTCAAGAAGAAC+AGG	0.465556	1.1:+75192226	MS.gene80088:CDS
AGTCGTCAAAGAAGGAGTTA+TGG	0.477577	1.1:+75192375	MS.gene80088:CDS
AGTTGTCAAAGAAGGAATGA+TGG	0.479247	1.1:+75192633	MS.gene80088:CDS
GGCAAGAAGATGTTGAATCT+TGG	0.485703	1.1:-75192427	None:intergenic
GGCAAGAAGGTGTTGAATCC+CGG	0.486543	1.1:-75192685	None:intergenic
AGATAAGATGGAAATGTTCA+AGG	0.489189	1.1:+75192819	MS.gene80088:CDS
ACATGAAATCTCACTTCCAT+TGG	0.500530	1.1:+75192456	MS.gene80088:CDS
ACACAAAATCTCAATTCCAT+TGG	0.505572	1.1:+75192714	MS.gene80088:CDS
GATAGAAGAGTCGTCAAAGA+AGG	0.515587	1.1:+75192367	MS.gene80088:CDS
TTCAACATCTTCTTGCCACC+AGG	0.516818	1.1:+75192433	MS.gene80088:CDS
ACACAAAATCTCACTTCCAT+TGG	0.521284	1.1:+75192972	MS.gene80088:CDS
CGCCCTTGCATCGTCAATCA+CGG	0.521774	1.1:-75192251	None:intergenic
GATGTAAGAGTTGTCAAAGA+AGG	0.540360	1.1:+75192625	MS.gene80088:CDS
GATGTAAGAGTTATGAAAGA+AGG	0.540360	1.1:+75192883	MS.gene80088:CDS
AGCTGTGTTTCAAGATAAGA+GGG	0.542710	1.1:+75192291	MS.gene80088:CDS
GTCCCGTGATTGACGATGCA+AGG	0.545886	1.1:+75192248	MS.gene80088:CDS
GATGAACAACAAGATGAAGA+TGG	0.553215	1.1:+75193000	MS.gene80088:CDS
TGTTGTTCATCACCATCCAA+TGG	0.554632	1.1:-75192988	None:intergenic
TTTGTGTCCCTCTGGCAAGA+AGG	0.563533	1.1:-75192698	None:intergenic
TTTGTGTCCCTCTGGCAAGA+AGG	0.563533	1.1:-75192956	None:intergenic
GCCCTTGCATCGTCAATCAC+GGG	0.577829	1.1:-75192250	None:intergenic
TCCCGTGATTGACGATGCAA+GGG	0.580973	1.1:+75192249	MS.gene80088:CDS
TTCAACACCTTCTTGCCAGA+GGG	0.592249	1.1:+75192691	MS.gene80088:CDS
TTCAACACCTTCTTGCCAGA+GGG	0.592249	1.1:+75192949	MS.gene80088:CDS
AAAATCTCACTTCCATTGGA+TGG	0.592611	1.1:+75192976	MS.gene80088:CDS
TGTTGTAAATCATCATCCAA+TGG	0.600709	1.1:-75192730	None:intergenic
ACCGCTCGAAGATGAAGCAA+TGG	0.613500	1.1:+75192507	MS.gene80088:CDS
GAGTTGAAAGATGCACAGAA+AGG	0.613537	1.1:+75193027	MS.gene80088:CDS
GGAAGTGAGATTTCATGTCC+TGG	0.614261	1.1:-75192451	None:intergenic
ACCATTGCTTCATCTTCGAG+CGG	0.637751	1.1:-75192508	None:intergenic
TGTTGTAGATCATCATCCAA+TGG	0.641812	1.1:-75192472	None:intergenic
ATTCAACACCTTCTTGCCAG+AGG	0.655191	1.1:+75192690	MS.gene80088:CDS
ATTCAACACCTTCTTGCCAG+AGG	0.655191	1.1:+75192948	MS.gene80088:CDS
AGTGAGATTTCATGTCCTGG+TGG	0.759321	1.1:-75192448	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGACCATAAAAATTTACTTC+CGG	+	chr1.1:75192666-75192685	MS.gene80088:CDS	25.0%
!	ATCCCAAAAGTAAATTTGTA+TGG	-	chr1.1:75192930-75192949	None:intergenic	25.0%
!	GGTTACATTTAAAGATAAGA+TGG	+	chr1.1:75192807-75192826	MS.gene80088:CDS	25.0%
!!	AACTCTTACATCAATTCTTT+TGG	-	chr1.1:75192617-75192636	None:intergenic	25.0%
!!	AGACCATACAAATTTACTTT+TGG	+	chr1.1:75192924-75192943	MS.gene80088:CDS	25.0%
!!!	GACCATACAAATTTACTTTT+GGG	+	chr1.1:75192925-75192944	MS.gene80088:CDS	25.0%
	ACACAAAATCTCAATTCCAT+TGG	+	chr1.1:75192714-75192733	MS.gene80088:CDS	30.0%
	AGATAAGATGGAAATGTTCA+AGG	+	chr1.1:75192819-75192838	MS.gene80088:CDS	30.0%
	AGTTATGAAAGAAGGAATGA+TGG	+	chr1.1:75192891-75192910	MS.gene80088:CDS	30.0%
	GACCATAAAAATTTACTTCC+GGG	+	chr1.1:75192667-75192686	MS.gene80088:CDS	30.0%
	GATGTAAGAGTTATGAAAGA+AGG	+	chr1.1:75192883-75192902	MS.gene80088:CDS	30.0%
	GGTTACATTTGAAGATAAGA+TGG	+	chr1.1:75192549-75192568	MS.gene80088:CDS	30.0%
	TGTTGTAAATCATCATCCAA+TGG	-	chr1.1:75192733-75192752	None:intergenic	30.0%
!	AACTCTTACATCAAGTCTTT+TGG	-	chr1.1:75192875-75192894	None:intergenic	30.0%
!	AGCATTTTTAACAGCAGTTA+AGG	+	chr1.1:75192786-75192805	MS.gene80088:CDS	30.0%
!	ATCCCGGAAGTAAATTTTTA+TGG	-	chr1.1:75192672-75192691	None:intergenic	30.0%
!	GACTCTTCTATCAATTCTTT+TGG	-	chr1.1:75192359-75192378	None:intergenic	30.0%
!	TTACATCAATTCTTTTGGCA+CGG	-	chr1.1:75192612-75192631	None:intergenic	30.0%
	AAAATCTCACTTCCATTGGA+TGG	+	chr1.1:75192976-75192995	MS.gene80088:CDS	35.0%
	AAGCTGTGTTTCAAGATAAG+AGG	+	chr1.1:75192290-75192309	MS.gene80088:CDS	35.0%
	ACACAAAATCTCACTTCCAT+TGG	+	chr1.1:75192972-75192991	MS.gene80088:CDS	35.0%
	ACATGAAATCTCACTTCCAT+TGG	+	chr1.1:75192456-75192475	MS.gene80088:CDS	35.0%
	AGCTGTGTTTCAAGATAAGA+GGG	+	chr1.1:75192291-75192310	MS.gene80088:CDS	35.0%
	AGTTGTCAAAGAAGGAATGA+TGG	+	chr1.1:75192633-75192652	MS.gene80088:CDS	35.0%
	GATGAACAACAAGATGAAGA+TGG	+	chr1.1:75193000-75193019	MS.gene80088:CDS	35.0%
	GATGTAAGAGTTGTCAAAGA+AGG	+	chr1.1:75192625-75192644	MS.gene80088:CDS	35.0%
	GTGAAACTCCTACAAGATTT+CGG	+	chr1.1:75192331-75192350	MS.gene80088:CDS	35.0%
	TGTTGTAGATCATCATCCAA+TGG	-	chr1.1:75192475-75192494	None:intergenic	35.0%
!	TTACATCAAGTCTTTTGGCA+TGG	-	chr1.1:75192870-75192889	None:intergenic	35.0%
!!	GGTGTTTTTAACAGCAGTTA+AGG	+	chr1.1:75192528-75192547	MS.gene80088:CDS	35.0%
	AGTCGTCAAAGAAGGAGTTA+TGG	+	chr1.1:75192375-75192394	MS.gene80088:CDS	40.0%
	ATGAACAGCTCAAGAAGAAC+AGG	+	chr1.1:75192226-75192245	MS.gene80088:CDS	40.0%
	GAGTTGAAAGATGCACAGAA+AGG	+	chr1.1:75193027-75193046	MS.gene80088:CDS	40.0%
	GATAGAAGAGTCGTCAAAGA+AGG	+	chr1.1:75192367-75192386	MS.gene80088:CDS	40.0%
	GGCAAGAAGATGTTGAATCT+TGG	-	chr1.1:75192430-75192449	None:intergenic	40.0%
	TGTTGTTCATCACCATCCAA+TGG	-	chr1.1:75192991-75193010	None:intergenic	40.0%
!	ATTGAGATTTTGTGTCCCTC+TGG	-	chr1.1:75192709-75192728	None:intergenic	40.0%
!	TTTTGGCATGGTAATCAGCT+AGG	-	chr1.1:75192858-75192877	None:intergenic	40.0%
	ACCATTGCTTCATCTTCGAG+CGG	-	chr1.1:75192511-75192530	None:intergenic	45.0%
	AGTGAGATTTCATGTCCTGG+TGG	-	chr1.1:75192451-75192470	None:intergenic	45.0%
	ATTCAACACCTTCTTGCCAG+AGG	+	chr1.1:75192690-75192709	MS.gene80088:CDS	45.0%
	GGAAGTGAGATTTCATGTCC+TGG	-	chr1.1:75192454-75192473	None:intergenic	45.0%
	TTCAACACCTTCTTGCCAGA+GGG	+	chr1.1:75192691-75192710	MS.gene80088:CDS	45.0%
	TTCAACATCTTCTTGCCACC+AGG	+	chr1.1:75192433-75192452	MS.gene80088:CDS	45.0%
!	AGTGAGATTTTGTGTCCCTC+TGG	-	chr1.1:75192967-75192986	None:intergenic	45.0%
!	TTTTGGCACGGTAATCAGCT+AGG	-	chr1.1:75192600-75192619	None:intergenic	45.0%
!!	TTTTGGCGCCGAAATCTTGT+AGG	-	chr1.1:75192342-75192361	None:intergenic	45.0%
	ACCGCTCGAAGATGAAGCAA+TGG	+	chr1.1:75192507-75192526	MS.gene80088:CDS	50.0%
	TCCCGTGATTGACGATGCAA+GGG	+	chr1.1:75192249-75192268	MS.gene80088:CDS	50.0%
	TTTGTGTCCCTCTGGCAAGA+AGG	-	chr1.1:75192701-75192720	None:intergenic	50.0%
!!	CGATGCAAGGGCGTTTGTAA+AGG	+	chr1.1:75192261-75192280	MS.gene80088:CDS	50.0%
!!	GGCAAGAAGGTGTTGAATCC+CGG	-	chr1.1:75192688-75192707	None:intergenic	50.0%
	CGCCCTTGCATCGTCAATCA+CGG	-	chr1.1:75192254-75192273	None:intergenic	55.0%
	GCCCTTGCATCGTCAATCAC+GGG	-	chr1.1:75192253-75192272	None:intergenic	55.0%
	GTCCCGTGATTGACGATGCA+AGG	+	chr1.1:75192248-75192267	MS.gene80088:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	75192226	75193062	75192226	ID=MS.gene80088
chr1.1	mRNA	75192226	75193062	75192226	ID=MS.gene80088.t1;Parent=MS.gene80088
chr1.1	exon	75192226	75193062	75192226	ID=MS.gene80088.t1.exon1;Parent=MS.gene80088.t1
chr1.1	CDS	75192226	75193062	75192226	ID=cds.MS.gene80088.t1;Parent=MS.gene80088.t1

Gene Sequence

>MS.gene80088

ATGAACAGCTCAAGAAGAACAGGTCCCGTGATTGACGATGCAAGGGCGTTTGTAAAGGACGTAGAAGCTGTGTTTCAAGATAAGAGGGAAAAGTATGATGAATTCGTGAAACTCCTACAAGATTTCGGCGCCAAAAGAATTGATAGAAGAGTCGTCAAAGAAGGAGTTATGGAGTTGTTTAAAGATCATCAAGATTTACTTCCAAGATTCAACATCTTCTTGCCACCAGGACATGAAATCTCACTTCCATTGGATGATGATCTACAACAACATGATCGATCACCGCTCGAAGATGAAGCAATGGTGTTTTTAACAGCAGTTAAGGTTACATTTGAAGATAAGATGGAAAAGTTCAATGAATTCTTGAAACTCCTAGCTGATTACCGTGCCAAAAGAATTGATGTAAGAGTTGTCAAAGAAGGAATGATGGAGTTGTTTAAAGACCATAAAAATTTACTTCCGGGATTCAACACCTTCTTGCCAGAGGGACACAAAATCTCAATTCCATTGGATGATGATTTACAACAACGCGATCGATCAGCACTCGAAGATGAAGCAACAGCATTTTTAACAGCAGTTAAGGTTACATTTAAAGATAAGATGGAAATGTTCAAGGAATTCTTGAAACTCCTAGCTGATTACCATGCCAAAAGACTTGATGTAAGAGTTATGAAAGAAGGAATGATGGAGTTGTTTAAAGACCATACAAATTTACTTTTGGGATTCAACACCTTCTTGCCAGAGGGACACAAAATCTCACTTCCATTGGATGGTGATGAACAACAAGATGAAGATGGATCAGAGTTGAAAGATGCACAGAAAGGTGATGAACAATAA

Protein sequence

>MS.gene80088.t1

MNSSRRTGPVIDDARAFVKDVEAVFQDKREKYDEFVKLLQDFGAKRIDRRVVKEGVMELFKDHQDLLPRFNIFLPPGHEISLPLDDDLQQHDRSPLEDEAMVFLTAVKVTFEDKMEKFNEFLKLLADYRAKRIDVRVVKEGMMELFKDHKNLLPGFNTFLPEGHKISIPLDDDLQQRDRSALEDEATAFLTAVKVTFKDKMEMFKEFLKLLADYHAKRLDVRVMKEGMMELFKDHTNLLLGFNTFLPEGHKISLPLDGDEQQDEDGSELKDAQKGDEQ