AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene71600


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71600.t1 MTR_3g006065 49.091 165 70 3 6 159 5 166 1.03e-38 130
MS.gene71600.t1 MTR_3g006185 46.108 167 74 4 9 159 8 174 3.20e-38 129
MS.gene71600.t1 MTR_4g133300 45.679 162 80 1 6 159 86 247 5.98e-38 130
MS.gene71600.t1 MTR_4g133300 47.059 153 73 3 8 155 11 160 2.43e-36 126
MS.gene71600.t1 MTR_3g006150 49.359 156 65 3 9 153 8 160 1.35e-37 126
MS.gene71600.t1 MTR_3g006150 46.774 62 33 0 94 155 15 76 1.08e-12 62.4
MS.gene71600.t1 MTR_1g109550 49.650 143 62 3 1 134 1 142 5.22e-37 124
MS.gene71600.t1 MTR_1g109550 56.061 66 28 1 86 151 18 82 1.59e-17 74.7
MS.gene71600.t1 MTR_4g133470 45.062 162 81 1 6 159 86 247 6.81e-36 125
MS.gene71600.t1 MTR_4g133470 45.752 153 75 3 8 155 11 160 5.12e-34 120
MS.gene71600.t1 MTR_3g006125 44.048 168 77 3 9 159 89 256 5.38e-35 122
MS.gene71600.t1 MTR_1g105955 44.218 147 78 2 10 152 17 163 8.63e-35 120
MS.gene71600.t1 MTR_1g105955 52.055 73 35 0 5 77 91 163 1.71e-19 80.9
MS.gene71600.t1 MTR_1g105955 52.308 65 31 0 83 147 15 79 1.38e-16 73.2
MS.gene71600.t1 MTR_1g104960 42.767 159 80 2 8 155 12 170 2.31e-33 116
MS.gene71600.t1 MTR_1g104960 46.667 75 40 0 4 78 94 168 1.73e-15 70.1
MS.gene71600.t1 MTR_3g006105 42.604 169 80 3 9 160 8 176 1.00e-32 115
MS.gene71600.t1 MTR_8g062700 41.333 150 77 1 8 146 41 190 3.65e-30 115
MS.gene71600.t1 MTR_8g062700 60.000 70 28 0 85 154 43 112 1.23e-21 90.9
MS.gene71600.t1 MTR_8g062700 41.333 150 77 1 8 146 41 190 3.72e-30 115
MS.gene71600.t1 MTR_8g062700 60.000 70 28 0 85 154 43 112 1.25e-21 90.9
MS.gene71600.t1 MTR_1g104980 39.456 147 81 2 11 155 1 141 8.66e-28 102
MS.gene71600.t1 MTR_6g008970 38.667 150 81 1 8 146 62 211 2.20e-26 104
MS.gene71600.t1 MTR_6g008970 57.971 69 29 0 85 153 64 132 2.10e-19 84.3
MS.gene71600.t1 MTR_5g036670 37.086 151 84 1 8 147 42 192 4.96e-26 103
MS.gene71600.t1 MTR_5g036670 57.971 69 29 0 85 153 44 112 2.83e-20 87.0
MS.gene71600.t1 MTR_7g013380 39.130 161 85 5 9 156 99 259 1.14e-24 97.1
MS.gene71600.t1 MTR_7g013380 37.975 158 85 5 10 154 14 171 5.37e-21 87.0
MS.gene71600.t1 MTR_7g013380 45.946 74 38 2 83 154 12 85 5.15e-11 59.7
MS.gene71600.t1 MTR_1g105960 46.429 112 45 2 10 121 2 98 1.60e-23 89.4
MS.gene71600.t1 MTR_1g105960 55.072 69 31 0 84 152 1 69 9.68e-20 79.7
MS.gene71600.t1 MTR_6g008995 36.054 147 85 1 9 146 37 183 1.88e-23 95.5
MS.gene71600.t1 MTR_6g008995 52.174 69 33 0 85 153 38 106 5.48e-17 77.4
MS.gene71600.t1 MTR_1g093310 57.500 80 26 2 1 79 1 73 6.32e-23 86.7
MS.gene71600.t1 MTR_1g093310 56.944 72 25 1 87 158 12 77 2.05e-19 77.8
MS.gene71600.t1 MTR_2g097830 47.525 101 45 2 8 103 69 166 1.93e-21 85.5
MS.gene71600.t1 MTR_7g013410 33.571 140 87 1 13 146 2 141 1.73e-19 79.7
MS.gene71600.t1 MTR_7g013410 46.269 67 36 0 88 154 2 68 4.12e-15 68.6
MS.gene71600.t1 MTR_1g091047 61.538 65 24 1 8 71 8 72 3.77e-19 77.0
MS.gene71600.t1 MTR_1g091047 50.000 60 29 1 88 146 13 72 3.78e-11 56.2
MS.gene71600.t1 MTR_6g008995 33.871 124 73 1 32 146 3 126 4.51e-17 77.4
MS.gene71600.t1 MTR_1g104940 46.667 75 40 0 81 155 11 85 5.78e-17 72.8
MS.gene71600.t1 MTR_1g104940 49.296 71 36 0 9 79 14 84 3.45e-16 70.9
MS.gene71600.t1 MTR_1g100260 55.556 72 28 2 11 78 26 97 1.54e-15 69.3
MS.gene71600.t1 MTR_1g100260 47.887 71 31 2 88 153 28 97 6.73e-12 59.7
MS.gene71600.t1 MTR_1g104937 46.479 71 38 0 85 155 13 83 5.50e-15 67.4
MS.gene71600.t1 MTR_1g104937 43.662 71 40 0 9 79 12 82 2.76e-11 57.8
MS.gene71600.t1 MTR_8g062350 29.016 193 81 4 7 146 12 201 1.75e-14 68.2
MS.gene71600.t1 MTR_8g062350 43.478 69 39 0 85 153 15 83 3.59e-13 64.7
MS.gene71600.t1 MTR_1g105010 44.118 68 38 0 85 152 17 84 3.40e-14 65.5
MS.gene71600.t1 MTR_1g105010 45.714 70 38 0 8 77 15 84 6.23e-14 64.7
MS.gene71600.t1 MTR_3g006010 52.632 57 27 0 98 154 23 79 8.44e-14 68.2
MS.gene71600.t1 MTR_3g006010 47.222 72 37 1 9 79 8 79 3.90e-13 66.2
MS.gene71600.t1 MTR_7g089490 45.833 72 39 0 81 152 13 84 1.35e-13 63.9
MS.gene71600.t1 MTR_5g073430 48.052 77 32 3 7 81 9 79 2.74e-13 63.2
MS.gene71600.t1 MTR_1g106560 50.000 64 16 2 98 161 12 59 1.83e-11 56.6
MS.gene71600.t1 MTR_1g023820 50.000 62 27 2 42 99 20 81 6.08e-11 57.0
MS.gene71600.t1 MTR_7g083950 51.667 60 28 1 87 145 16 75 7.66e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene71600.t1 AT5G15020 42.568 148 76 2 8 146 51 198 4.36e-28 109
MS.gene71600.t1 AT5G15020 57.971 69 29 0 85 153 53 121 4.12e-19 83.6
MS.gene71600.t1 AT5G15020 42.568 148 76 2 8 146 51 198 4.44e-28 109
MS.gene71600.t1 AT5G15020 57.971 69 29 0 85 153 53 121 4.20e-19 83.6
MS.gene71600.t1 AT1G24190 38.667 150 81 1 8 146 13 162 1.11e-27 108
MS.gene71600.t1 AT1G24190 56.522 69 30 0 85 153 15 83 8.32e-19 82.8
MS.gene71600.t1 AT1G24190 38.667 150 81 1 8 146 13 162 1.47e-27 107
MS.gene71600.t1 AT1G24190 56.522 69 30 0 85 153 15 83 1.01e-18 82.4
MS.gene71600.t1 AT1G24190 38.667 150 81 1 8 146 13 162 1.56e-27 107
MS.gene71600.t1 AT1G24190 56.522 69 30 0 85 153 15 83 1.06e-18 82.4
MS.gene71600.t1 AT1G70060 38.411 151 81 1 8 146 13 163 9.24e-27 105
MS.gene71600.t1 AT1G70060 56.522 69 30 0 85 153 15 83 6.25e-18 80.5
MS.gene71600.t1 AT1G70060 38.411 151 81 1 8 146 13 163 9.24e-27 105
MS.gene71600.t1 AT1G70060 56.522 69 30 0 85 153 15 83 6.25e-18 80.5
MS.gene71600.t1 AT1G59890 38.776 147 81 1 9 146 45 191 1.29e-26 105
MS.gene71600.t1 AT1G59890 59.420 69 28 0 85 153 46 114 1.89e-20 87.4
MS.gene71600.t1 AT1G59890 38.776 147 81 1 9 146 45 191 1.53e-26 105
MS.gene71600.t1 AT1G59890 59.420 69 28 0 85 153 46 114 2.08e-20 87.4
MS.gene71600.t1 AT1G59890 38.776 147 81 1 9 146 45 191 1.75e-26 104
MS.gene71600.t1 AT1G59890 59.420 69 28 0 85 153 46 114 2.27e-20 87.4
MS.gene71600.t1 AT1G59890 38.776 147 81 1 9 146 45 191 1.75e-26 104
MS.gene71600.t1 AT1G59890 59.420 69 28 0 85 153 46 114 2.27e-20 87.4
MS.gene71600.t1 AT3G01320 36.913 149 84 1 8 146 56 204 4.52e-26 103
MS.gene71600.t1 AT3G01320 57.971 69 29 0 85 153 58 126 5.33e-20 86.3
MS.gene71600.t1 AT3G01320 36.913 149 84 1 8 146 56 204 4.57e-26 103
MS.gene71600.t1 AT3G01320 57.971 69 29 0 85 153 58 126 5.33e-20 86.3
MS.gene71600.t1 AT1G10450 38.776 147 81 2 9 146 84 230 8.87e-26 102
MS.gene71600.t1 AT1G10450 53.623 69 32 0 85 153 85 153 2.08e-17 79.0
MS.gene71600.t1 AT1G10450 38.776 147 81 2 9 146 33 179 1.03e-25 102
MS.gene71600.t1 AT1G10450 53.623 69 32 0 85 153 34 102 2.33e-17 78.6
MS.gene71600.t1 AT1G10450 38.776 147 81 2 9 146 33 179 1.03e-25 102
MS.gene71600.t1 AT1G10450 53.623 69 32 0 85 153 34 102 2.33e-17 78.6
MS.gene71600.t1 AT1G24230 33.333 150 95 2 8 156 13 158 1.37e-25 98.2
MS.gene71600.t1 AT1G24200 33.333 147 90 1 8 146 13 159 4.89e-22 87.8
MS.gene71600.t1 AT1G24200 48.529 68 35 0 85 152 15 82 4.36e-14 67.0
MS.gene71600.t1 AT1G27260 32.335 167 98 2 10 161 4 170 9.68e-21 85.1
MS.gene71600.t1 AT1G27260 28.148 135 84 2 8 141 92 214 5.22e-11 59.3
MS.gene71600.t1 AT1G70030 34.459 148 86 2 9 146 11 157 1.19e-20 83.2
MS.gene71600.t1 AT1G70030 45.714 70 38 0 85 154 12 81 1.20e-13 65.1
MS.gene71600.t1 AT1G23810 29.213 178 106 3 4 161 9 186 1.29e-19 82.4
MS.gene71600.t1 AT1G23810 32.353 136 79 2 8 142 108 231 8.61e-18 77.8
MS.gene71600.t1 AT1G24250 33.094 139 80 2 8 145 119 245 2.37e-18 79.3
MS.gene71600.t1 AT5G15025 53.623 69 32 0 85 153 48 116 7.19e-17 72.4
MS.gene71600.t1 AT5G15025 49.296 71 36 0 8 78 46 116 8.12e-14 64.7
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.73e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.17e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.94e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.19e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.94e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.19e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.95e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.20e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.95e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.20e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.95e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.20e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 241 328 8.96e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 242 309 1.20e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.97e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.20e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.97e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.20e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.98e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.20e-15 73.9
MS.gene71600.t1 AT4G12020 46.067 89 47 1 9 97 308 395 8.98e-17 77.0
MS.gene71600.t1 AT4G12020 49.275 69 34 1 85 153 309 376 1.20e-15 73.9
MS.gene71600.t1 AT1G70030 31.159 138 72 1 9 146 22 136 2.70e-14 66.2
MS.gene71600.t1 AT1G70030 43.210 81 42 1 77 153 11 91 5.04e-14 65.5
MS.gene71600.t1 AT1G27270 30.769 169 93 3 6 150 11 179 2.99e-14 68.2
MS.gene71600.t1 AT1G70030 43.210 81 42 1 77 153 11 91 3.45e-14 65.9
MS.gene71600.t1 AT1G70030 45.714 70 38 0 9 78 22 91 3.24e-13 63.2
MS.gene71600.t1 AT1G27250 45.333 75 41 0 2 76 14 88 3.59e-14 65.9
MS.gene71600.t1 AT1G27250 49.206 63 32 0 85 147 22 84 7.14e-14 65.1
MS.gene71600.t1 AT1G27220 37.895 95 59 0 8 102 13 107 1.93e-13 65.1
MS.gene71600.t1 AT1G27220 37.838 74 46 0 85 158 15 88 1.12e-12 63.2
MS.gene71600.t1 AT1G27280 26.207 145 94 1 27 158 11 155 1.24e-12 63.5
MS.gene71600.t1 AT1G24210 31.250 144 88 3 6 148 11 144 1.62e-11 59.3

Find 25 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCTGAATTTCAATATAAC+AGG 0.361061 1.4:-82027621 MS.gene71600:CDS
AGAGTGAAGAGGTTGTTTAA+AGG 0.379464 1.4:-82027523 MS.gene71600:CDS
AGAATGAAGAAGCTGCTTAA+AGG 0.383126 1.4:-82027298 MS.gene71600:CDS
CCAGTGGTCTTAAGTTGAAA+TGG 0.385161 1.4:+82027214 None:intergenic
AGTTGCAACAACATTTCTTA+AGG 0.390449 1.4:-82027425 MS.gene71600:CDS
CAAAGTTATGAGGGATTTCA+AGG 0.391778 1.4:-82027353 MS.gene71600:CDS
CATCAAATCACACTTCCATC+AGG 0.438813 1.4:-82027454 MS.gene71600:CDS
AATGTATAGATGAAGATCTC+TGG 0.452496 1.4:-82027682 None:intergenic
TTCCATCTCTGAAATCTAGT+AGG 0.457834 1.4:+82027572 None:intergenic
AGTGTGATTTGATGTTCCTT+TGG 0.466154 1.4:+82027463 None:intergenic
AAGCTGCGTTTCAAGATAAC+GGG 0.467722 1.4:-82027396 MS.gene71600:CDS
GAAGCTGCGTTTCAAGATAA+CGG 0.480662 1.4:-82027397 MS.gene71600:CDS
AGTGTGATTCGATGCTGTTT+TGG 0.491674 1.4:+82027238 None:intergenic
CCATTTCAACTTAAGACCAC+TGG 0.499321 1.4:-82027214 MS.gene71600:CDS
TTGATGTTCCTTTGGTAGGA+AGG 0.503740 1.4:+82027471 None:intergenic
CTCCTACTAGATTTCAGAGA+TGG 0.525821 1.4:-82027574 MS.gene71600:CDS
TGGAAACCGCGATGATGCAT+TGG 0.530913 1.4:-82027662 MS.gene71600:CDS
TGATTTGATGTTCCTTTGGT+AGG 0.550872 1.4:+82027467 None:intergenic
TTGTCAAAGCAAGAGTGAAG+AGG 0.596584 1.4:-82027534 MS.gene71600:CDS
ATCAAATCACACTTCCATCA+GGG 0.599894 1.4:-82027453 MS.gene71600:CDS
GTTGCAACTTTAAACCCTGA+TGG 0.629202 1.4:+82027439 None:intergenic
AATTGATACACAATATGTCG+TGG 0.632933 1.4:-82027323 MS.gene71600:CDS
ATTCAACACCTTCCTACCAA+AGG 0.639776 1.4:-82027479 MS.gene71600:CDS
TGCAATGAACTACTAACCAG+TGG 0.676230 1.4:+82027198 None:intergenic
GATACGCCAATGCATCATCG+CGG 0.757377 1.4:+82027656 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATCCAAAAATTAAATCTGCA+TGG + chr1.4:82027386-82027405 None:intergenic 25.0%
!!! ATCAGTTTTTCAAAGTTATG+AGG - chr1.4:82027520-82027539 MS.gene71600:CDS 25.0%
!!! TCAGTTTTTCAAAGTTATGA+GGG - chr1.4:82027521-82027540 MS.gene71600:CDS 25.0%
AATTGATACACAATATGTCG+TGG - chr1.4:82027560-82027579 MS.gene71600:CDS 30.0%
AGTTGCAACAACATTTCTTA+AGG - chr1.4:82027458-82027477 MS.gene71600:CDS 30.0%
GAGCTGAATTTCAATATAAC+AGG - chr1.4:82027262-82027281 MS.gene71600:CDS 30.0%
AGAATGAAGAAGCTGCTTAA+AGG - chr1.4:82027585-82027604 MS.gene71600:CDS 35.0%
AGAGTGAAGAGGTTGTTTAA+AGG - chr1.4:82027360-82027379 MS.gene71600:CDS 35.0%
AGTGTGATTTGATGTTCCTT+TGG + chr1.4:82027423-82027442 None:intergenic 35.0%
ATCAAATCACACTTCCATCA+GGG - chr1.4:82027430-82027449 MS.gene71600:CDS 35.0%
CAAAGTTATGAGGGATTTCA+AGG - chr1.4:82027530-82027549 MS.gene71600:CDS 35.0%
TGATTTGATGTTCCTTTGGT+AGG + chr1.4:82027419-82027438 None:intergenic 35.0%
!! GGCCATGCAGATTTAATTTT+TGG - chr1.4:82027381-82027400 MS.gene71600:CDS 35.0%
!! TTCCATCTCTGAAATCTAGT+AGG + chr1.4:82027314-82027333 None:intergenic 35.0%
AAGCTGCGTTTCAAGATAAC+GGG - chr1.4:82027487-82027506 MS.gene71600:CDS 40.0%
ATTCAACACCTTCCTACCAA+AGG - chr1.4:82027404-82027423 MS.gene71600:CDS 40.0%
CATCAAATCACACTTCCATC+AGG - chr1.4:82027429-82027448 MS.gene71600:CDS 40.0%
CCATTTCAACTTAAGACCAC+TGG - chr1.4:82027669-82027688 MS.gene71600:CDS 40.0%
CTCCTACTAGATTTCAGAGA+TGG - chr1.4:82027309-82027328 MS.gene71600:CDS 40.0%
GAAGCTGCGTTTCAAGATAA+CGG - chr1.4:82027486-82027505 MS.gene71600:CDS 40.0%
GTTGCAACTTTAAACCCTGA+TGG + chr1.4:82027447-82027466 None:intergenic 40.0%
TTGATGTTCCTTTGGTAGGA+AGG + chr1.4:82027415-82027434 None:intergenic 40.0%
TTGTCAAAGCAAGAGTGAAG+AGG - chr1.4:82027349-82027368 MS.gene71600:CDS 40.0%
!! AGTGTGATTCGATGCTGTTT+TGG + chr1.4:82027648-82027667 None:intergenic 40.0%
!! CCAGTGGTCTTAAGTTGAAA+TGG + chr1.4:82027672-82027691 None:intergenic 40.0%
!!! TGATTCGATGCTGTTTTGGC+AGG + chr1.4:82027644-82027663 None:intergenic 45.0%
GATACGCCAATGCATCATCG+CGG + chr1.4:82027230-82027249 None:intergenic 50.0%
TGGAAACCGCGATGATGCAT+TGG - chr1.4:82027221-82027240 MS.gene71600:CDS 50.0%
!!! TCGATGCTGTTTTGGCAGGA+AGG + chr1.4:82027640-82027659 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 82027210 82027695 82027210 ID=MS.gene71600
chr1.4 mRNA 82027210 82027695 82027210 ID=MS.gene71600.t1;Parent=MS.gene71600
chr1.4 exon 82027210 82027695 82027210 ID=MS.gene71600.t1.exon1;Parent=MS.gene71600.t1
chr1.4 CDS 82027210 82027695 82027210 ID=cds.MS.gene71600.t1;Parent=MS.gene71600.t1
Gene Sequence

>MS.gene71600

ATGAAGATCTCTGGAAACCGCGATGATGCATTGGCGTATCTTAAAGCAGTAAGAGCTGAATTTCAATATAACAGGATAAAGTATGATGAGTTGTTAAAACTCCTACTAGATTTCAGAGATGGAAAAATTGATATAAGAGTTGTCAAAGCAAGAGTGAAGAGGTTGTTTAAAGGCCATGCAGATTTAATTTTTGGATTCAACACCTTCCTACCAAAGGAACATCAAATCACACTTCCATCAGGGTTTAAAGTTGCAACAACATTTCTTAAGGAAGTAGAAGCTGCGTTTCAAGATAACGGGAAAAAGTATGATCAGTTTTTCAAAGTTATGAGGGATTTCAAGGATCAAAGAATTGATACACAATATGTCGTGGCAAGAATGAAGAAGCTGCTTAAAGGACATTCAGATTTAATTTTGAGATTCAACACCTTCCTGCCAAAACAGCATCGAATCACACTTCCATTTCAACTTAAGACCACTGGTTAG

Protein sequence

>MS.gene71600.t1

MKISGNRDDALAYLKAVRAEFQYNRIKYDELLKLLLDFRDGKIDIRVVKARVKRLFKGHADLIFGFNTFLPKEHQITLPSGFKVATTFLKEVEAAFQDNGKKYDQFFKVMRDFKDQRIDTQYVVARMKKLLKGHSDLILRFNTFLPKQHRITLPFQLKTTG