AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93864


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93864.t1 MTR_1g104960 68.715 179 50 1 1 179 1 173 1.35e-81 240
MS.gene93864.t1 MTR_1g104960 62.245 98 36 1 93 190 2 98 8.62e-31 111
MS.gene93864.t1 MTR_1g104960 61.333 75 29 0 14 88 100 174 1.43e-25 97.8
MS.gene93864.t1 MTR_1g104980 65.482 197 33 4 15 197 1 176 4.90e-78 231
MS.gene93864.t1 MTR_4g133300 45.783 166 81 1 13 178 89 245 1.17e-40 138
MS.gene93864.t1 MTR_4g133300 40.314 191 96 4 1 190 1 174 1.05e-27 105
MS.gene93864.t1 MTR_1g104950 75.269 93 9 2 36 126 1 81 6.81e-40 131
MS.gene93864.t1 MTR_1g104950 67.797 59 17 1 128 184 1 59 1.45e-18 76.6
MS.gene93864.t1 MTR_4g133470 46.108 167 79 3 13 178 89 245 5.89e-39 134
MS.gene93864.t1 MTR_4g133470 41.146 192 93 6 1 190 1 174 3.52e-27 103
MS.gene93864.t1 MTR_3g006125 44.318 176 84 4 11 179 87 255 1.29e-36 128
MS.gene93864.t1 MTR_3g006125 45.745 94 45 2 103 190 87 180 4.10e-14 68.9
MS.gene93864.t1 MTR_1g104937 61.290 93 36 0 6 98 5 97 5.32e-36 122
MS.gene93864.t1 MTR_1g104937 63.441 93 34 0 101 193 8 100 2.88e-33 115
MS.gene93864.t1 MTR_1g104940 61.957 92 35 0 105 196 14 105 8.50e-35 119
MS.gene93864.t1 MTR_1g104940 60.000 95 36 1 6 98 5 99 6.33e-32 112
MS.gene93864.t1 MTR_1g105955 38.953 172 92 1 2 173 5 163 7.96e-34 119
MS.gene93864.t1 MTR_3g006185 41.520 171 95 3 11 179 6 173 2.91e-33 117
MS.gene93864.t1 MTR_3g006185 47.312 93 44 2 103 190 6 98 1.62e-13 65.9
MS.gene93864.t1 MTR_3g006105 41.477 176 89 4 11 179 6 174 6.61e-32 114
MS.gene93864.t1 MTR_3g006105 47.872 94 43 2 103 190 6 99 3.23e-17 75.9
MS.gene93864.t1 MTR_3g006150 42.262 168 86 3 8 174 3 160 9.15e-32 113
MS.gene93864.t1 MTR_3g006150 47.674 86 42 1 105 190 8 90 2.80e-18 78.2
MS.gene93864.t1 MTR_3g006150 48.684 76 36 2 4 78 83 156 2.84e-11 59.7
MS.gene93864.t1 MTR_3g006065 41.040 173 89 4 12 181 7 169 1.21e-29 108
MS.gene93864.t1 MTR_3g006065 44.186 86 45 1 105 190 8 90 8.22e-15 69.7
MS.gene93864.t1 MTR_5g036670 40.625 160 89 1 8 167 38 191 4.40e-29 113
MS.gene93864.t1 MTR_5g036670 50.633 79 38 1 103 181 41 118 5.30e-18 81.6
MS.gene93864.t1 MTR_8g062700 38.125 160 93 1 8 167 37 190 3.35e-26 105
MS.gene93864.t1 MTR_8g062700 52.174 92 42 2 91 181 27 117 5.86e-21 90.5
MS.gene93864.t1 MTR_8g062700 38.125 160 93 1 8 167 37 190 3.48e-26 105
MS.gene93864.t1 MTR_8g062700 52.174 92 42 2 91 181 27 117 5.98e-21 90.5
MS.gene93864.t1 MTR_7g013380 35.928 167 99 3 13 177 99 259 4.36e-25 99.4
MS.gene93864.t1 MTR_7g013380 34.637 179 105 3 1 175 1 171 7.97e-25 98.6
MS.gene93864.t1 MTR_7g013380 38.947 95 53 2 13 105 185 276 3.16e-12 64.3
MS.gene93864.t1 MTR_6g008970 36.875 160 95 1 8 167 58 211 3.44e-23 96.7
MS.gene93864.t1 MTR_6g008970 46.154 78 42 0 103 180 61 138 9.76e-15 72.4
MS.gene93864.t1 MTR_6g008995 35.484 155 92 1 13 167 37 183 1.45e-22 94.4
MS.gene93864.t1 MTR_6g008995 43.836 73 41 0 105 177 37 109 2.48e-13 68.2
MS.gene93864.t1 MTR_1g105960 48.052 77 40 0 106 182 2 78 1.12e-17 75.1
MS.gene93864.t1 MTR_1g105960 35.433 127 50 1 14 140 2 96 2.43e-17 74.3
MS.gene93864.t1 MTR_1g105010 50.000 82 36 1 97 173 3 84 1.54e-16 72.4
MS.gene93864.t1 MTR_1g105010 47.826 69 36 0 13 81 16 84 1.05e-13 65.1
MS.gene93864.t1 MTR_1g109550 36.806 144 75 3 10 153 13 140 2.60e-16 72.8
MS.gene93864.t1 MTR_1g109550 44.872 78 34 2 113 190 24 92 4.15e-11 58.9
MS.gene93864.t1 MTR_6g008995 33.835 133 80 1 35 167 2 126 5.38e-15 72.8
MS.gene93864.t1 MTR_8g062350 43.820 89 49 1 1 88 1 89 3.18e-14 68.6
MS.gene93864.t1 MTR_8g062350 40.964 83 47 1 104 186 13 93 3.08e-12 63.2
MS.gene93864.t1 MTR_7g013410 26.797 153 97 2 17 167 2 141 1.14e-13 65.5
MS.gene93864.t1 MTR_7g013410 45.455 66 36 0 110 175 3 68 5.27e-11 58.5
MS.gene93864.t1 MTR_3g006010 48.000 75 38 1 103 176 6 80 1.17e-12 65.9
MS.gene93864.t1 MTR_3g006010 44.000 75 41 1 11 84 6 80 1.99e-11 62.4
MS.gene93864.t1 MTR_1g093310 42.857 77 36 2 104 179 8 77 2.05e-12 60.5
MS.gene93864.t1 MTR_1g091047 50.746 67 32 1 104 169 8 74 2.74e-12 60.1
MS.gene93864.t1 MTR_2g097830 46.667 75 37 1 101 175 66 137 7.27e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93864.t1 AT1G24200 36.025 161 94 1 8 168 9 160 1.28e-24 95.9
MS.gene93864.t1 AT1G24200 46.988 83 41 1 91 173 3 82 6.94e-17 75.5
MS.gene93864.t1 AT1G70060 38.415 164 96 1 4 167 5 163 1.91e-24 100
MS.gene93864.t1 AT1G70060 50.000 72 36 0 103 174 12 83 1.66e-15 74.7
MS.gene93864.t1 AT1G70060 38.415 164 96 1 4 167 5 163 1.91e-24 100
MS.gene93864.t1 AT1G70060 50.000 72 36 0 103 174 12 83 1.66e-15 74.7
MS.gene93864.t1 AT3G01320 36.782 174 97 3 4 175 46 208 2.01e-24 100
MS.gene93864.t1 AT3G01320 36.782 174 97 3 4 175 46 208 2.09e-24 100
MS.gene93864.t1 AT1G24190 36.207 174 94 3 8 175 9 171 5.26e-23 96.3
MS.gene93864.t1 AT1G24190 46.429 84 42 1 91 174 3 83 7.10e-16 75.9
MS.gene93864.t1 AT1G24190 36.207 174 94 3 8 175 9 171 5.36e-23 96.3
MS.gene93864.t1 AT1G24190 46.429 84 42 1 91 174 3 83 6.90e-16 75.9
MS.gene93864.t1 AT1G24190 36.207 174 94 3 8 175 9 171 5.37e-23 96.3
MS.gene93864.t1 AT1G24190 46.429 84 42 1 91 174 3 83 6.58e-16 75.9
MS.gene93864.t1 AT1G27260 35.912 181 113 2 11 191 1 178 5.60e-23 92.0
MS.gene93864.t1 AT1G23810 32.044 181 119 3 13 190 14 193 7.30e-23 92.4
MS.gene93864.t1 AT1G23810 31.008 129 82 2 3 131 99 220 1.76e-12 64.3
MS.gene93864.t1 AT1G24250 31.088 193 118 3 13 191 14 205 2.56e-22 91.3
MS.gene93864.t1 AT5G15020 36.774 155 90 2 13 167 52 198 3.30e-22 94.0
MS.gene93864.t1 AT5G15020 45.833 72 39 0 103 174 50 121 5.20e-14 70.5
MS.gene93864.t1 AT5G15020 36.774 155 90 2 13 167 52 198 3.50e-22 94.0
MS.gene93864.t1 AT5G15020 45.833 72 39 0 103 174 50 121 5.36e-14 70.1
MS.gene93864.t1 AT1G10450 33.129 163 101 2 9 171 80 234 1.14e-19 86.7
MS.gene93864.t1 AT1G10450 44.706 85 43 2 90 174 73 153 1.61e-13 68.9
MS.gene93864.t1 AT1G10450 33.129 163 101 2 9 171 29 183 1.56e-19 86.3
MS.gene93864.t1 AT1G10450 44.706 85 43 2 90 174 22 102 1.79e-13 68.6
MS.gene93864.t1 AT1G10450 33.129 163 101 2 9 171 29 183 1.56e-19 86.3
MS.gene93864.t1 AT1G10450 44.706 85 43 2 90 174 22 102 1.79e-13 68.6
MS.gene93864.t1 AT1G24230 30.000 170 97 2 11 179 12 160 5.78e-19 82.0
MS.gene93864.t1 AT1G24230 25.949 158 100 3 7 160 80 224 3.67e-12 63.5
MS.gene93864.t1 AT1G27280 34.375 160 101 2 24 183 4 159 9.81e-19 80.9
MS.gene93864.t1 AT1G70030 33.750 160 98 3 8 167 6 157 1.17e-18 79.3
MS.gene93864.t1 AT1G70030 43.836 73 41 0 105 177 11 83 1.08e-11 60.8
MS.gene93864.t1 AT1G59890 32.903 155 96 1 13 167 45 191 1.32e-18 83.6
MS.gene93864.t1 AT1G59890 47.143 70 37 0 105 174 45 114 8.64e-14 69.7
MS.gene93864.t1 AT1G59890 32.903 155 96 1 13 167 45 191 1.42e-18 83.6
MS.gene93864.t1 AT1G59890 47.143 70 37 0 105 174 45 114 9.14e-14 69.7
MS.gene93864.t1 AT1G59890 32.903 155 96 1 13 167 45 191 1.42e-18 83.6
MS.gene93864.t1 AT1G59890 47.143 70 37 0 105 174 45 114 9.06e-14 69.7
MS.gene93864.t1 AT1G59890 32.903 155 96 1 13 167 45 191 1.62e-18 83.2
MS.gene93864.t1 AT1G59890 47.143 70 37 0 105 174 45 114 9.67e-14 69.3
MS.gene93864.t1 AT1G27270 34.783 161 98 3 13 166 14 174 2.09e-15 72.4
MS.gene93864.t1 AT1G70030 37.097 124 69 3 13 134 22 138 3.47e-14 67.0
MS.gene93864.t1 AT1G70030 44.286 70 39 0 105 174 22 91 8.42e-12 60.5
MS.gene93864.t1 AT5G35610 48.750 80 38 1 113 192 18 94 9.34e-14 66.2
MS.gene93864.t1 AT5G35610 43.750 80 42 1 1 80 1 77 2.00e-11 60.1
MS.gene93864.t1 AT1G27220 41.892 74 43 0 8 81 9 82 1.51e-13 66.2
MS.gene93864.t1 AT1G27220 38.202 89 52 1 91 179 3 88 7.69e-13 64.3
MS.gene93864.t1 AT5G15040 40.260 77 46 0 105 181 3 79 3.57e-13 62.8
MS.gene93864.t1 AT5G15040 35.227 88 55 1 11 98 1 86 5.78e-11 57.0
MS.gene93864.t1 AT5G15040 40.260 77 46 0 105 181 3 79 3.57e-13 62.8
MS.gene93864.t1 AT5G15040 35.227 88 55 1 11 98 1 86 5.78e-11 57.0
MS.gene93864.t1 AT5G15040 40.260 77 46 0 105 181 3 79 3.57e-13 62.8
MS.gene93864.t1 AT5G15040 35.227 88 55 1 11 98 1 86 5.78e-11 57.0
MS.gene93864.t1 AT1G70030 37.383 107 60 2 13 119 22 121 7.60e-13 63.2
MS.gene93864.t1 AT1G70030 43.836 73 41 0 105 177 22 94 4.21e-12 61.2
MS.gene93864.t1 AT5G15025 37.209 86 54 0 2 87 36 121 4.70e-12 60.8
MS.gene93864.t1 AT1G24210 43.243 74 40 1 5 76 4 77 5.81e-11 58.9

Find 33 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCTTATATCAATTCTTT+TGG 0.302742 1.2:-77883236 None:intergenic
AGAGCATCAAGATTTACTTT+TGG 0.331136 1.2:+77883288 MS.gene93864:CDS
CTCCATTGCATCACCAATCA+AGG 0.409244 1.2:-77882855 None:intergenic
AGTTGTCAAAGAAGGAGTTA+TGG 0.423391 1.2:+77882979 MS.gene93864:CDS
AACTTGTGTTTCAAGATAAG+AGG 0.425933 1.2:+77882894 MS.gene93864:CDS
AAGTTGCATTTCAAAATAAG+AGG 0.445054 1.2:+77883170 MS.gene93864:CDS
TGATGCAATGGAGTTTATAA+AGG 0.447186 1.2:+77882865 MS.gene93864:CDS
AATAAAAGAGTTGTCAAAGA+AGG 0.468195 1.2:+77882971 MS.gene93864:CDS
AGAAGAACAAAACCCTTGAT+TGG 0.483812 1.2:+77882842 MS.gene93864:CDS
AGTTGTCAAAGAAGGAGTGA+TGG 0.486303 1.2:+77883255 MS.gene93864:CDS
TGAAATCTCATTTCCATTGG+AGG 0.501735 1.2:+77883063 MS.gene93864:CDS
ACATGAAATCTCATTTCCAT+TGG 0.503318 1.2:+77883060 MS.gene93864:CDS
CTCAACACTTTCTTGCCAGC+GGG 0.511799 1.2:+77883313 MS.gene93864:CDS
GATATAAGAGTTGTCAAAGA+AGG 0.512296 1.2:+77883247 MS.gene93864:CDS
GATGGATCAGTGCTCAAAGA+TGG 0.516579 1.2:+77883103 MS.gene93864:CDS
TTCAACATCTTCTTGCCACC+AGG 0.516818 1.2:+77883037 MS.gene93864:CDS
GATGATCAACAACGAGGTGA+TGG 0.517956 1.2:+77883085 MS.gene93864:CDS
ACACGAAATCTCACTTCCAT+TGG 0.519367 1.2:+77883336 MS.gene93864:CDS
AGGTGATGGATTAAAATTCA+AGG 0.528999 1.2:+77883375 MS.gene93864:CDS
ACTTGTGTTTCAAGATAAGA+GGG 0.530583 1.2:+77882895 MS.gene93864:CDS
AGTGAGATTTCGTGTCCCGC+TGG 0.542973 1.2:-77883328 None:intergenic
ATTGCTTCATCTATGAGTGC+TGG 0.548558 1.2:-77883127 None:intergenic
AGTTGCATTTCAAAATAAGA+GGG 0.555199 1.2:+77883171 MS.gene93864:CDS
TGTTGTTCATCAATATCCAA+TGG 0.559496 1.2:-77883352 None:intergenic
ATTGATGAACAACAAGGTGA+TGG 0.570660 1.2:+77883361 MS.gene93864:CDS
ACCCTTGATTGGTGATGCAA+TGG 0.572570 1.2:+77882853 MS.gene93864:CDS
CGTTGTTGATCATCCTCCAA+TGG 0.582098 1.2:-77883076 None:intergenic
TCCATTGCATCACCAATCAA+GGG 0.592756 1.2:-77882854 None:intergenic
GGAAATGAGATTTCATGTCC+TGG 0.602274 1.2:-77883055 None:intergenic
ACTCAACACTTTCTTGCCAG+CGG 0.612834 1.2:+77883312 MS.gene93864:CDS
TTGGATATTGATGAACAACA+AGG 0.734900 1.2:+77883355 MS.gene93864:CDS
AATGAGATTTCATGTCCTGG+TGG 0.753374 1.2:-77883052 None:intergenic
TTGGAGGATGATCAACAACG+AGG 0.806393 1.2:+77883079 MS.gene93864:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTCTTTTATTAATTCTTT+TGG - chr1.2:77882963-77882982 None:intergenic 15.0%
!!! AACTCTTATATCAATTCTTT+TGG - chr1.2:77883239-77883258 None:intergenic 20.0%
! AAGTTGCATTTCAAAATAAG+AGG + chr1.2:77883170-77883189 MS.gene93864:CDS 25.0%
! AATAAAAGAGTTGTCAAAGA+AGG + chr1.2:77882971-77882990 MS.gene93864:CDS 25.0%
! AGTTGCATTTCAAAATAAGA+GGG + chr1.2:77883171-77883190 MS.gene93864:CDS 25.0%
AACTTGTGTTTCAAGATAAG+AGG + chr1.2:77882894-77882913 MS.gene93864:CDS 30.0%
ACATGAAATCTCATTTCCAT+TGG + chr1.2:77883060-77883079 MS.gene93864:CDS 30.0%
ACTTGTGTTTCAAGATAAGA+GGG + chr1.2:77882895-77882914 MS.gene93864:CDS 30.0%
GATATAAGAGTTGTCAAAGA+AGG + chr1.2:77883247-77883266 MS.gene93864:CDS 30.0%
TGATGCAATGGAGTTTATAA+AGG + chr1.2:77882865-77882884 MS.gene93864:CDS 30.0%
TGTTGTTCATCAATATCCAA+TGG - chr1.2:77883355-77883374 None:intergenic 30.0%
TTGGATATTGATGAACAACA+AGG + chr1.2:77883355-77883374 MS.gene93864:CDS 30.0%
! TTTTGGCATTGTAATCAACT+AGG - chr1.2:77883222-77883241 None:intergenic 30.0%
!! AGAGCATCAAGATTTACTTT+TGG + chr1.2:77883288-77883307 MS.gene93864:CDS 30.0%
!! AGGTGATGGATTAAAATTCA+AGG + chr1.2:77883375-77883394 MS.gene93864:CDS 30.0%
!! GAGCATCAAGATTTACTTTT+GGG + chr1.2:77883289-77883308 MS.gene93864:CDS 30.0%
AGAAGAACAAAACCCTTGAT+TGG + chr1.2:77882842-77882861 MS.gene93864:CDS 35.0%
AGTTGTCAAAGAAGGAGTTA+TGG + chr1.2:77882979-77882998 MS.gene93864:CDS 35.0%
ATTGATGAACAACAAGGTGA+TGG + chr1.2:77883361-77883380 MS.gene93864:CDS 35.0%
TGAAATCTCATTTCCATTGG+AGG + chr1.2:77883063-77883082 MS.gene93864:CDS 35.0%
! TTTTGGCACTGTAATCATGT+AGG - chr1.2:77882946-77882965 None:intergenic 35.0%
!!! AATCAAGGGTTTTGTTCTTC+TGG - chr1.2:77882843-77882862 None:intergenic 35.0%
AATGAGATTTCATGTCCTGG+TGG - chr1.2:77883055-77883074 None:intergenic 40.0%
ACACGAAATCTCACTTCCAT+TGG + chr1.2:77883336-77883355 MS.gene93864:CDS 40.0%
AGTTGTCAAAGAAGGAGTGA+TGG + chr1.2:77883255-77883274 MS.gene93864:CDS 40.0%
ATTGCTTCATCTATGAGTGC+TGG - chr1.2:77883130-77883149 None:intergenic 40.0%
GGAAATGAGATTTCATGTCC+TGG - chr1.2:77883058-77883077 None:intergenic 40.0%
TCCATTGCATCACCAATCAA+GGG - chr1.2:77882857-77882876 None:intergenic 40.0%
ACTCAACACTTTCTTGCCAG+CGG + chr1.2:77883312-77883331 MS.gene93864:CDS 45.0%
CGTTGTTGATCATCCTCCAA+TGG - chr1.2:77883079-77883098 None:intergenic 45.0%
CTCCATTGCATCACCAATCA+AGG - chr1.2:77882858-77882877 None:intergenic 45.0%
GATGATCAACAACGAGGTGA+TGG + chr1.2:77883085-77883104 MS.gene93864:CDS 45.0%
GATGGATCAGTGCTCAAAGA+TGG + chr1.2:77883103-77883122 MS.gene93864:CDS 45.0%
TTCAACATCTTCTTGCCACC+AGG + chr1.2:77883037-77883056 MS.gene93864:CDS 45.0%
TTGGAGGATGATCAACAACG+AGG + chr1.2:77883079-77883098 MS.gene93864:CDS 45.0%
!! ACCCTTGATTGGTGATGCAA+TGG + chr1.2:77882853-77882872 MS.gene93864:CDS 45.0%
CTCAACACTTTCTTGCCAGC+GGG + chr1.2:77883313-77883332 MS.gene93864:CDS 50.0%
AGTGAGATTTCGTGTCCCGC+TGG - chr1.2:77883331-77883350 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 77882830 77883423 77882830 ID=MS.gene93864
chr1.2 mRNA 77882830 77883423 77882830 ID=MS.gene93864.t1;Parent=MS.gene93864
chr1.2 exon 77882830 77883423 77882830 ID=MS.gene93864.t1.exon1;Parent=MS.gene93864.t1
chr1.2 CDS 77882830 77883423 77882830 ID=cds.MS.gene93864.t1;Parent=MS.gene93864.t1
Gene Sequence

>MS.gene93864

ATGAACAGCTCCAGAAGAACAAAACCCTTGATTGGTGATGCAATGGAGTTTATAAAGGATGTAAAACTTGTGTTTCAAGATAAGAGGGAAAAGTTTGATGAATTCATGAAACTCCTACATGATTACAGTGCCAAAAGAATTAATAAAAGAGTTGTCAAAGAAGGAGTTATGGAGTTGTTGAAAGAACATCAAGATTTAATTTCAAGATTCAACATCTTCTTGCCACCAGGACATGAAATCTCATTTCCATTGGAGGATGATCAACAACGAGGTGATGGATCAGTGCTCAAAGATGGACCAGCACTCATAGATGAAGCAATGTCGCTTGTAACAGCAGTTAAAGTTGCATTTCAAAATAAGAGGGAAAAGTATGATGAATTCTTGAAACTCCTAGTTGATTACAATGCCAAAAGAATTGATATAAGAGTTGTCAAAGAAGGAGTGATGGAGTTGTTTAAAGAGCATCAAGATTTACTTTTGGGACTCAACACTTTCTTGCCAGCGGGACACGAAATCTCACTTCCATTGGATATTGATGAACAACAAGGTGATGGATTAAAATTCAAGGATGAACAGAAAGATGATGAGCAATAA

Protein sequence

>MS.gene93864.t1

MNSSRRTKPLIGDAMEFIKDVKLVFQDKREKFDEFMKLLHDYSAKRINKRVVKEGVMELLKEHQDLISRFNIFLPPGHEISFPLEDDQQRGDGSVLKDGPALIDEAMSLVTAVKVAFQNKREKYDEFLKLLVDYNAKRIDIRVVKEGVMELFKEHQDLLLGLNTFLPAGHEISLPLDIDEQQGDGLKFKDEQKDDEQ