AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80087


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80087.t1 MTR_1g104960 68.156 179 51 1 1 179 1 173 2.11e-81 239
MS.gene80087.t1 MTR_1g104960 62.245 98 36 1 93 190 2 98 5.31e-31 111
MS.gene80087.t1 MTR_1g104960 61.333 75 29 0 14 88 100 174 1.40e-25 97.8
MS.gene80087.t1 MTR_1g104980 64.467 197 35 3 15 197 1 176 2.51e-78 232
MS.gene80087.t1 MTR_4g133300 45.783 166 81 1 13 178 89 245 1.05e-40 139
MS.gene80087.t1 MTR_4g133300 40.314 191 96 4 1 190 1 174 5.12e-28 105
MS.gene80087.t1 MTR_1g104950 75.269 93 9 2 36 126 1 81 6.45e-40 131
MS.gene80087.t1 MTR_1g104950 67.797 59 17 1 128 184 1 59 1.31e-18 77.0
MS.gene80087.t1 MTR_4g133470 46.108 167 79 3 13 178 89 245 6.02e-39 134
MS.gene80087.t1 MTR_4g133470 41.146 192 93 6 1 190 1 174 2.03e-27 104
MS.gene80087.t1 MTR_3g006125 44.886 176 83 4 11 179 87 255 8.23e-37 129
MS.gene80087.t1 MTR_3g006125 45.745 94 45 2 103 190 87 180 5.72e-14 68.6
MS.gene80087.t1 MTR_1g104937 60.215 93 37 0 6 98 5 97 7.37e-36 122
MS.gene80087.t1 MTR_1g104937 63.441 93 34 0 101 193 8 100 1.32e-33 116
MS.gene80087.t1 MTR_1g104940 61.957 92 35 0 105 196 14 105 1.02e-34 119
MS.gene80087.t1 MTR_1g104940 62.069 87 33 0 12 98 13 99 7.28e-32 112
MS.gene80087.t1 MTR_1g105955 38.953 172 92 1 2 173 5 163 8.77e-34 119
MS.gene80087.t1 MTR_3g006185 42.105 171 94 3 11 179 6 173 1.79e-33 117
MS.gene80087.t1 MTR_3g006185 47.312 93 44 2 103 190 6 98 1.01e-13 66.6
MS.gene80087.t1 MTR_3g006105 42.045 176 88 4 11 179 6 174 3.81e-32 114
MS.gene80087.t1 MTR_3g006105 47.872 94 43 2 103 190 6 99 4.43e-17 75.9
MS.gene80087.t1 MTR_3g006150 42.262 168 86 3 8 174 3 160 9.45e-32 113
MS.gene80087.t1 MTR_3g006150 47.674 86 42 1 105 190 8 90 4.10e-18 77.8
MS.gene80087.t1 MTR_3g006150 47.368 76 37 2 4 78 83 156 4.33e-11 59.3
MS.gene80087.t1 MTR_3g006065 41.040 173 89 4 12 181 7 169 1.07e-29 108
MS.gene80087.t1 MTR_3g006065 44.186 86 45 1 105 190 8 90 1.34e-14 69.3
MS.gene80087.t1 MTR_5g036670 40.625 160 89 1 8 167 38 191 3.27e-29 114
MS.gene80087.t1 MTR_5g036670 50.633 79 38 1 103 181 41 118 4.72e-18 82.0
MS.gene80087.t1 MTR_8g062700 38.125 160 93 1 8 167 37 190 2.61e-26 105
MS.gene80087.t1 MTR_8g062700 52.174 92 42 2 91 181 27 117 5.81e-21 90.5
MS.gene80087.t1 MTR_8g062700 38.125 160 93 1 8 167 37 190 2.69e-26 105
MS.gene80087.t1 MTR_8g062700 52.174 92 42 2 91 181 27 117 5.59e-21 90.5
MS.gene80087.t1 MTR_7g013380 35.928 167 99 3 13 177 99 259 3.43e-25 99.8
MS.gene80087.t1 MTR_7g013380 35.196 179 104 3 1 175 1 171 3.80e-25 99.4
MS.gene80087.t1 MTR_7g013380 38.947 95 53 2 13 105 185 276 2.95e-12 64.3
MS.gene80087.t1 MTR_6g008970 36.875 160 95 1 8 167 58 211 3.09e-23 97.1
MS.gene80087.t1 MTR_6g008970 46.154 78 42 0 103 180 61 138 9.58e-15 72.4
MS.gene80087.t1 MTR_6g008995 35.484 155 92 1 13 167 37 183 1.48e-22 94.4
MS.gene80087.t1 MTR_6g008995 43.836 73 41 0 105 177 37 109 2.52e-13 67.8
MS.gene80087.t1 MTR_1g105960 48.052 77 40 0 106 182 2 78 1.15e-17 75.1
MS.gene80087.t1 MTR_1g105960 35.433 127 50 1 14 140 2 96 2.53e-17 74.3
MS.gene80087.t1 MTR_1g105010 50.000 82 36 1 97 173 3 84 1.31e-16 72.8
MS.gene80087.t1 MTR_1g105010 47.826 69 36 0 13 81 16 84 9.68e-14 65.1
MS.gene80087.t1 MTR_1g109550 34.722 144 78 2 10 153 13 140 2.02e-16 72.8
MS.gene80087.t1 MTR_1g109550 50.000 60 29 1 113 172 24 82 6.54e-11 58.2
MS.gene80087.t1 MTR_6g008995 33.835 133 80 1 35 167 2 126 4.89e-15 72.8
MS.gene80087.t1 MTR_8g062350 43.820 89 49 1 1 88 1 89 2.04e-14 68.9
MS.gene80087.t1 MTR_8g062350 40.964 83 47 1 104 186 13 93 2.73e-12 63.2
MS.gene80087.t1 MTR_7g013410 26.797 153 97 2 17 167 2 141 1.09e-13 65.9
MS.gene80087.t1 MTR_7g013410 45.455 66 36 0 110 175 3 68 5.11e-11 58.5
MS.gene80087.t1 MTR_3g006010 48.000 75 38 1 103 176 6 80 9.89e-13 66.2
MS.gene80087.t1 MTR_3g006010 44.000 75 41 1 11 84 6 80 2.19e-11 62.4
MS.gene80087.t1 MTR_1g093310 42.857 77 36 2 104 179 8 77 2.09e-12 60.5
MS.gene80087.t1 MTR_1g091047 50.746 67 32 1 104 169 8 74 2.45e-12 60.5
MS.gene80087.t1 MTR_2g097830 42.553 94 50 2 101 193 66 156 5.34e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80087.t1 AT1G24200 36.025 161 94 1 8 168 9 160 1.00e-24 96.3
MS.gene80087.t1 AT1G24200 46.988 83 41 1 91 173 3 82 6.38e-17 75.5
MS.gene80087.t1 AT3G01320 36.782 174 97 3 4 175 46 208 1.45e-24 100
MS.gene80087.t1 AT1G70060 38.415 164 96 1 4 167 5 163 1.49e-24 100
MS.gene80087.t1 AT1G70060 50.000 72 36 0 103 174 12 83 1.57e-15 74.7
MS.gene80087.t1 AT1G70060 38.415 164 96 1 4 167 5 163 1.49e-24 100
MS.gene80087.t1 AT1G70060 50.000 72 36 0 103 174 12 83 1.57e-15 74.7
MS.gene80087.t1 AT3G01320 36.782 174 97 3 4 175 46 208 1.71e-24 100
MS.gene80087.t1 AT1G24190 36.207 174 94 3 8 175 9 171 4.38e-23 96.7
MS.gene80087.t1 AT1G24190 46.429 84 42 1 91 174 3 83 6.83e-16 75.9
MS.gene80087.t1 AT1G24190 36.207 174 94 3 8 175 9 171 4.56e-23 96.7
MS.gene80087.t1 AT1G24190 46.429 84 42 1 91 174 3 83 7.10e-16 75.9
MS.gene80087.t1 AT1G24190 36.207 174 94 3 8 175 9 171 4.78e-23 96.3
MS.gene80087.t1 AT1G24190 46.429 84 42 1 91 174 3 83 6.65e-16 75.9
MS.gene80087.t1 AT1G27260 37.209 172 105 2 14 185 4 172 6.42e-23 92.0
MS.gene80087.t1 AT1G23810 32.386 176 115 3 13 185 14 188 9.69e-23 92.0
MS.gene80087.t1 AT1G23810 30.233 129 83 2 3 131 99 220 2.97e-12 63.9
MS.gene80087.t1 AT5G15020 36.774 155 90 2 13 167 52 198 3.60e-22 94.0
MS.gene80087.t1 AT5G15020 45.833 72 39 0 103 174 50 121 5.30e-14 70.1
MS.gene80087.t1 AT5G15020 36.774 155 90 2 13 167 52 198 3.82e-22 94.0
MS.gene80087.t1 AT5G15020 45.833 72 39 0 103 174 50 121 5.41e-14 70.1
MS.gene80087.t1 AT1G24250 31.551 187 113 3 13 185 14 199 3.93e-22 90.5
MS.gene80087.t1 AT1G10450 33.129 163 101 2 9 171 80 234 8.34e-20 87.0
MS.gene80087.t1 AT1G10450 44.706 85 43 2 90 174 73 153 1.49e-13 68.9
MS.gene80087.t1 AT1G10450 33.129 163 101 2 9 171 29 183 1.11e-19 86.7
MS.gene80087.t1 AT1G10450 44.706 85 43 2 90 174 22 102 1.67e-13 68.9
MS.gene80087.t1 AT1G10450 33.129 163 101 2 9 171 29 183 1.11e-19 86.7
MS.gene80087.t1 AT1G10450 44.706 85 43 2 90 174 22 102 1.67e-13 68.9
MS.gene80087.t1 AT1G27280 33.537 164 105 2 24 187 4 163 9.12e-19 81.3
MS.gene80087.t1 AT1G70030 33.750 160 98 3 8 167 6 157 9.89e-19 79.3
MS.gene80087.t1 AT1G70030 43.836 73 41 0 105 177 11 83 1.04e-11 60.8
MS.gene80087.t1 AT1G24230 29.412 170 98 2 11 179 12 160 1.14e-18 81.3
MS.gene80087.t1 AT1G24230 26.316 152 95 3 13 160 86 224 4.06e-12 63.5
MS.gene80087.t1 AT1G59890 32.903 155 96 1 13 167 45 191 1.24e-18 83.6
MS.gene80087.t1 AT1G59890 47.143 70 37 0 105 174 45 114 8.40e-14 69.7
MS.gene80087.t1 AT1G59890 32.903 155 96 1 13 167 45 191 1.28e-18 83.6
MS.gene80087.t1 AT1G59890 47.143 70 37 0 105 174 45 114 8.63e-14 69.7
MS.gene80087.t1 AT1G59890 32.903 155 96 1 13 167 45 191 1.29e-18 83.6
MS.gene80087.t1 AT1G59890 47.143 70 37 0 105 174 45 114 8.55e-14 69.7
MS.gene80087.t1 AT1G59890 32.903 155 96 1 13 167 45 191 1.29e-18 83.6
MS.gene80087.t1 AT1G59890 47.143 70 37 0 105 174 45 114 8.47e-14 69.7
MS.gene80087.t1 AT1G27270 34.783 161 98 3 13 166 14 174 2.37e-15 72.4
MS.gene80087.t1 AT1G70030 36.434 129 73 3 8 134 17 138 3.22e-14 67.0
MS.gene80087.t1 AT1G70030 44.286 70 39 0 105 174 22 91 8.17e-12 60.8
MS.gene80087.t1 AT1G27220 43.243 74 42 0 8 81 9 82 7.96e-14 67.0
MS.gene80087.t1 AT1G27220 38.202 89 52 1 91 179 3 88 6.59e-13 64.7
MS.gene80087.t1 AT5G15040 40.260 77 46 0 105 181 3 79 3.69e-13 62.8
MS.gene80087.t1 AT5G15040 35.227 88 55 1 11 98 1 86 7.19e-11 57.0
MS.gene80087.t1 AT5G15040 40.260 77 46 0 105 181 3 79 3.69e-13 62.8
MS.gene80087.t1 AT5G15040 35.227 88 55 1 11 98 1 86 7.19e-11 57.0
MS.gene80087.t1 AT5G15040 40.260 77 46 0 105 181 3 79 3.69e-13 62.8
MS.gene80087.t1 AT5G15040 35.227 88 55 1 11 98 1 86 7.19e-11 57.0
MS.gene80087.t1 AT5G35610 47.500 80 39 1 113 192 18 94 4.68e-13 64.3
MS.gene80087.t1 AT5G35610 43.750 80 42 1 1 80 1 77 2.08e-11 60.1
MS.gene80087.t1 AT1G70030 36.607 112 64 2 8 119 17 121 5.73e-13 63.5
MS.gene80087.t1 AT1G70030 43.836 73 41 0 105 177 22 94 3.87e-12 61.2
MS.gene80087.t1 AT5G15025 37.209 86 54 0 2 87 36 121 4.06e-12 61.2
MS.gene80087.t1 AT1G24210 43.243 74 40 1 5 76 4 77 8.24e-11 58.5

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCTTATATCAATTCTTT+TGG 0.302742 1.1:-75189913 None:intergenic
AGAGCATCAAGATTTACTTT+TGG 0.331136 1.1:+75189965 MS.gene80087:CDS
AGTTGTCAAAGAAGGAGTTA+TGG 0.423391 1.1:+75189656 MS.gene80087:CDS
AACTTGTGTTTCAAGATAAG+AGG 0.425933 1.1:+75189571 MS.gene80087:CDS
AAGTTGCATTTCAAAATAAG+AGG 0.445054 1.1:+75189847 MS.gene80087:CDS
TGATGCAATGGAGTTTATAA+AGG 0.447186 1.1:+75189542 MS.gene80087:CDS
AATAAAAGAGTTGTCAAAGA+AGG 0.468195 1.1:+75189648 MS.gene80087:CDS
AGTTGTCAAAGAAGGAGTGA+TGG 0.486303 1.1:+75189932 MS.gene80087:CDS
TGAAATCTCATTTCCATTGG+AGG 0.501735 1.1:+75189740 MS.gene80087:CDS
ACATGAAATCTCATTTCCAT+TGG 0.503318 1.1:+75189737 MS.gene80087:CDS
CTCAACACTTTCTTGCCAGC+GGG 0.511799 1.1:+75189990 MS.gene80087:CDS
GATATAAGAGTTGTCAAAGA+AGG 0.512296 1.1:+75189924 MS.gene80087:CDS
GATGGATCAGTGCTCAAAGA+TGG 0.516579 1.1:+75189780 MS.gene80087:CDS
TTCAACATCTTCTTGCCACC+AGG 0.516818 1.1:+75189714 MS.gene80087:CDS
GATGATCAACAACGAGGTGA+TGG 0.517956 1.1:+75189762 MS.gene80087:CDS
ACACGAAATCTCACTTCCAT+TGG 0.519367 1.1:+75190013 MS.gene80087:CDS
ACTTGTGTTTCAAGATAAGA+GGG 0.530583 1.1:+75189572 MS.gene80087:CDS
AGGTGATGGATTAACATTCA+AGG 0.537891 1.1:+75190052 MS.gene80087:CDS
AGTGAGATTTCGTGTCCCGC+TGG 0.542973 1.1:-75190005 None:intergenic
ATTGCTTCATCTATGAGTGC+TGG 0.548558 1.1:-75189804 None:intergenic
AGTTGCATTTCAAAATAAGA+GGG 0.555199 1.1:+75189848 MS.gene80087:CDS
CTCCAGAAGAACAAAACCCT+TGG 0.559303 1.1:+75189515 MS.gene80087:CDS
TGTTGTTCATCAATATCCAA+TGG 0.559496 1.1:-75190029 None:intergenic
CTCCATTGCATCACCAACCA+AGG 0.561324 1.1:-75189532 None:intergenic
ATTGATGAACAACAAGGTGA+TGG 0.570660 1.1:+75190038 MS.gene80087:CDS
ACCCTTGGTTGGTGATGCAA+TGG 0.572570 1.1:+75189530 MS.gene80087:CDS
CGTTGTTGATCATCCTCCAA+TGG 0.582098 1.1:-75189753 None:intergenic
AGAAGAACAAAACCCTTGGT+TGG 0.585689 1.1:+75189519 MS.gene80087:CDS
GGAAATGAGATTTCATGTCC+TGG 0.602274 1.1:-75189732 None:intergenic
ACTCAACACTTTCTTGCCAG+CGG 0.612834 1.1:+75189989 MS.gene80087:CDS
TCCATTGCATCACCAACCAA+GGG 0.622798 1.1:-75189531 None:intergenic
TTGGATATTGATGAACAACA+AGG 0.734900 1.1:+75190032 MS.gene80087:CDS
AATGAGATTTCATGTCCTGG+TGG 0.753374 1.1:-75189729 None:intergenic
TTGGAGGATGATCAACAACG+AGG 0.806393 1.1:+75189756 MS.gene80087:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTCTTTTATTAATTCTTT+TGG - chr1.1:75189640-75189659 None:intergenic 15.0%
!!! AACTCTTATATCAATTCTTT+TGG - chr1.1:75189916-75189935 None:intergenic 20.0%
! AAGTTGCATTTCAAAATAAG+AGG + chr1.1:75189847-75189866 MS.gene80087:CDS 25.0%
! AATAAAAGAGTTGTCAAAGA+AGG + chr1.1:75189648-75189667 MS.gene80087:CDS 25.0%
! AGTTGCATTTCAAAATAAGA+GGG + chr1.1:75189848-75189867 MS.gene80087:CDS 25.0%
AACTTGTGTTTCAAGATAAG+AGG + chr1.1:75189571-75189590 MS.gene80087:CDS 30.0%
ACATGAAATCTCATTTCCAT+TGG + chr1.1:75189737-75189756 MS.gene80087:CDS 30.0%
ACTTGTGTTTCAAGATAAGA+GGG + chr1.1:75189572-75189591 MS.gene80087:CDS 30.0%
GATATAAGAGTTGTCAAAGA+AGG + chr1.1:75189924-75189943 MS.gene80087:CDS 30.0%
TGATGCAATGGAGTTTATAA+AGG + chr1.1:75189542-75189561 MS.gene80087:CDS 30.0%
TGTTGTTCATCAATATCCAA+TGG - chr1.1:75190032-75190051 None:intergenic 30.0%
TTGGATATTGATGAACAACA+AGG + chr1.1:75190032-75190051 MS.gene80087:CDS 30.0%
! TTTTGGCATTGTAATCAACT+AGG - chr1.1:75189899-75189918 None:intergenic 30.0%
!! AGAGCATCAAGATTTACTTT+TGG + chr1.1:75189965-75189984 MS.gene80087:CDS 30.0%
!! GAGCATCAAGATTTACTTTT+GGG + chr1.1:75189966-75189985 MS.gene80087:CDS 30.0%
AGTTGTCAAAGAAGGAGTTA+TGG + chr1.1:75189656-75189675 MS.gene80087:CDS 35.0%
ATTGATGAACAACAAGGTGA+TGG + chr1.1:75190038-75190057 MS.gene80087:CDS 35.0%
TGAAATCTCATTTCCATTGG+AGG + chr1.1:75189740-75189759 MS.gene80087:CDS 35.0%
! TTTTGGCACTGTAATCATGT+AGG - chr1.1:75189623-75189642 None:intergenic 35.0%
!! AGGTGATGGATTAACATTCA+AGG + chr1.1:75190052-75190071 MS.gene80087:CDS 35.0%
AATGAGATTTCATGTCCTGG+TGG - chr1.1:75189732-75189751 None:intergenic 40.0%
ACACGAAATCTCACTTCCAT+TGG + chr1.1:75190013-75190032 MS.gene80087:CDS 40.0%
AGAAGAACAAAACCCTTGGT+TGG + chr1.1:75189519-75189538 MS.gene80087:CDS 40.0%
AGTTGTCAAAGAAGGAGTGA+TGG + chr1.1:75189932-75189951 MS.gene80087:CDS 40.0%
ATTGCTTCATCTATGAGTGC+TGG - chr1.1:75189807-75189826 None:intergenic 40.0%
GGAAATGAGATTTCATGTCC+TGG - chr1.1:75189735-75189754 None:intergenic 40.0%
!!! AACCAAGGGTTTTGTTCTTC+TGG - chr1.1:75189520-75189539 None:intergenic 40.0%
ACTCAACACTTTCTTGCCAG+CGG + chr1.1:75189989-75190008 MS.gene80087:CDS 45.0%
CGTTGTTGATCATCCTCCAA+TGG - chr1.1:75189756-75189775 None:intergenic 45.0%
CTCCAGAAGAACAAAACCCT+TGG + chr1.1:75189515-75189534 MS.gene80087:CDS 45.0%
GATGATCAACAACGAGGTGA+TGG + chr1.1:75189762-75189781 MS.gene80087:CDS 45.0%
GATGGATCAGTGCTCAAAGA+TGG + chr1.1:75189780-75189799 MS.gene80087:CDS 45.0%
TCCATTGCATCACCAACCAA+GGG - chr1.1:75189534-75189553 None:intergenic 45.0%
TTCAACATCTTCTTGCCACC+AGG + chr1.1:75189714-75189733 MS.gene80087:CDS 45.0%
TTGGAGGATGATCAACAACG+AGG + chr1.1:75189756-75189775 MS.gene80087:CDS 45.0%
CTCAACACTTTCTTGCCAGC+GGG + chr1.1:75189990-75190009 MS.gene80087:CDS 50.0%
CTCCATTGCATCACCAACCA+AGG - chr1.1:75189535-75189554 None:intergenic 50.0%
!! ACCCTTGGTTGGTGATGCAA+TGG + chr1.1:75189530-75189549 MS.gene80087:CDS 50.0%
AGTGAGATTTCGTGTCCCGC+TGG - chr1.1:75190008-75190027 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 75189507 75190100 75189507 ID=MS.gene80087
chr1.1 mRNA 75189507 75190100 75189507 ID=MS.gene80087.t1;Parent=MS.gene80087
chr1.1 exon 75189507 75190100 75189507 ID=MS.gene80087.t1.exon1;Parent=MS.gene80087.t1
chr1.1 CDS 75189507 75190100 75189507 ID=cds.MS.gene80087.t1;Parent=MS.gene80087.t1
Gene Sequence

>MS.gene80087

ATGAACAGCTCCAGAAGAACAAAACCCTTGGTTGGTGATGCAATGGAGTTTATAAAGGATGTAAAACTTGTGTTTCAAGATAAGAGGGAAAAGTTTGATGAATTCATGAAACTCCTACATGATTACAGTGCCAAAAGAATTAATAAAAGAGTTGTCAAAGAAGGAGTTATGGAGTTGTTGAAAGAACATCAAGATTTAATTTCAAGATTCAACATCTTCTTGCCACCAGGACATGAAATCTCATTTCCATTGGAGGATGATCAACAACGAGGTGATGGATCAGTGCTCAAAGATGGACCAGCACTCATAGATGAAGCAATGTCGCTTGTAACAGCAGTTAAAGTTGCATTTCAAAATAAGAGGGAAAAGTATGATGAATTCTTGAAACTCCTAGTTGATTACAATGCCAAAAGAATTGATATAAGAGTTGTCAAAGAAGGAGTGATGGAGTTGTTTAAAGAGCATCAAGATTTACTTTTGGGACTCAACACTTTCTTGCCAGCGGGACACGAAATCTCACTTCCATTGGATATTGATGAACAACAAGGTGATGGATTAACATTCAAGGATGAACAGAAAGATGATGAGCAATAA

Protein sequence

>MS.gene80087.t1

MNSSRRTKPLVGDAMEFIKDVKLVFQDKREKFDEFMKLLHDYSAKRINKRVVKEGVMELLKEHQDLISRFNIFLPPGHEISFPLEDDQQRGDGSVLKDGPALIDEAMSLVTAVKVAFQNKREKYDEFLKLLVDYNAKRIDIRVVKEGVMELFKEHQDLLLGLNTFLPAGHEISLPLDIDEQQGDGLTFKDEQKDDEQ