Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44406.t1 | XP_003592709.1 | 99.1 | 114 | 1 | 0 | 1 | 114 | 1 | 114 | 6.10E-51 | 209.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44406.t1 | A8IAP8 | 45.7 | 92 | 49 | 1 | 21 | 112 | 29 | 119 | 5.9e-10 | 65.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44406.t1 | G7IE75 | 99.1 | 114 | 1 | 0 | 1 | 114 | 1 | 114 | 4.4e-51 | 209.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049419 | MS.gene44406 | 0.828952 | 6.75E-55 | -1.69E-46 |
| MS.gene050105 | MS.gene44406 | -0.819809 | 9.55E-53 | -1.69E-46 |
| MS.gene050361 | MS.gene44406 | -0.803435 | 3.49E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene44406 | -0.804238 | 2.38E-49 | -1.69E-46 |
| MS.gene051122 | MS.gene44406 | -0.820309 | 7.34E-53 | -1.69E-46 |
| MS.gene051275 | MS.gene44406 | -0.807423 | 5.09E-50 | -1.69E-46 |
| MS.gene051856 | MS.gene44406 | -0.800988 | 1.11E-48 | -1.69E-46 |
| MS.gene05216 | MS.gene44406 | -0.836225 | 1.06E-56 | -1.69E-46 |
| MS.gene052674 | MS.gene44406 | -0.832805 | 7.67E-56 | -1.69E-46 |
| MS.gene052675 | MS.gene44406 | -0.831956 | 1.24E-55 | -1.69E-46 |
| MS.gene052677 | MS.gene44406 | -0.832077 | 1.16E-55 | -1.69E-46 |
| MS.gene054479 | MS.gene44406 | -0.816726 | 4.77E-52 | -1.69E-46 |
| MS.gene055063 | MS.gene44406 | 0.80017 | 1.63E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene44406 | -0.801447 | 8.97E-49 | -1.69E-46 |
| MS.gene056045 | MS.gene44406 | -0.800913 | 1.15E-48 | -1.69E-46 |
| MS.gene056064 | MS.gene44406 | -0.8124 | 4.32E-51 | -1.69E-46 |
| MS.gene056480 | MS.gene44406 | -0.819952 | 8.86E-53 | -1.69E-46 |
| MS.gene057156 | MS.gene44406 | 0.806534 | 7.85E-50 | -1.69E-46 |
| MS.gene058081 | MS.gene44406 | -0.809832 | 1.56E-50 | -1.69E-46 |
| MS.gene058223 | MS.gene44406 | 0.83474 | 2.52E-56 | -1.69E-46 |
| MS.gene060850 | MS.gene44406 | -0.824607 | 7.37E-54 | -1.69E-46 |
| MS.gene060851 | MS.gene44406 | -0.849804 | 2.56E-60 | -1.69E-46 |
| MS.gene060852 | MS.gene44406 | -0.847277 | 1.28E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44406.t1 | MTR_1g114200 | 99.123 | 114 | 1 | 0 | 1 | 114 | 1 | 114 | 2.37e-78 | 226 |
| MS.gene44406.t1 | MTR_4g032660 | 33.663 | 101 | 67 | 0 | 11 | 111 | 96 | 196 | 1.00e-16 | 72.4 |
| MS.gene44406.t1 | MTR_5g023360 | 70.833 | 48 | 14 | 0 | 1 | 48 | 46 | 93 | 2.11e-16 | 68.9 |
| MS.gene44406.t1 | MTR_3g012540 | 29.126 | 103 | 73 | 0 | 11 | 113 | 80 | 182 | 3.56e-13 | 62.8 |
| MS.gene44406.t1 | MTR_5g023330 | 75.000 | 32 | 8 | 0 | 1 | 32 | 88 | 119 | 2.47e-12 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44406.t1 | AT5G27820 | 79.825 | 114 | 23 | 0 | 1 | 114 | 1 | 114 | 3.83e-65 | 192 |
| MS.gene44406.t1 | AT3G20230 | 35.644 | 101 | 65 | 0 | 11 | 111 | 83 | 183 | 6.97e-19 | 77.8 |
| MS.gene44406.t1 | AT3G45020 | 36.937 | 111 | 58 | 4 | 16 | 114 | 4 | 114 | 3.29e-13 | 61.6 |
| MS.gene44406.t1 | AT3G45020 | 36.937 | 111 | 58 | 4 | 16 | 114 | 4 | 114 | 3.29e-13 | 61.6 |
| MS.gene44406.t1 | AT3G45020 | 36.937 | 111 | 58 | 4 | 16 | 114 | 4 | 114 | 3.29e-13 | 61.6 |
| MS.gene44406.t1 | AT3G45020 | 36.937 | 111 | 58 | 4 | 16 | 114 | 4 | 114 | 3.29e-13 | 61.6 |
| MS.gene44406.t1 | AT1G08845 | 27.184 | 103 | 75 | 0 | 11 | 113 | 82 | 184 | 2.00e-12 | 60.8 |
| MS.gene44406.t1 | AT1G08845 | 27.184 | 103 | 75 | 0 | 11 | 113 | 82 | 184 | 2.00e-12 | 60.8 |
| MS.gene44406.t1 | AT1G08845 | 27.184 | 103 | 75 | 0 | 11 | 113 | 127 | 229 | 2.71e-12 | 61.2 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAGATATTGTGTAAGTC+TGG | 0.313396 | 1.1:-80235791 | None:intergenic |
| CAACATCCCGAGTAGTTTCC+AGG | 0.336475 | 1.1:-80235930 | None:intergenic |
| CTGGGAGGCCTGACTGGAGG+TGG | 0.388017 | 1.1:-80235772 | None:intergenic |
| AAGCGGTTATTGATTCTCTC+AGG | 0.400454 | 1.1:+80236061 | MS.gene44406:CDS |
| GTAAGTCTGGGAGGCCTGAC+TGG | 0.402204 | 1.1:-80235778 | None:intergenic |
| AAATGCATCTTAAGAACATA+AGG | 0.422008 | 1.1:-80235814 | None:intergenic |
| GAAAGCCTTGAGATCAAGCC+TGG | 0.444737 | 1.1:+80235912 | MS.gene44406:CDS |
| CTCCTGTGAGCTTGCAGAAG+AGG | 0.447057 | 1.1:-80235890 | None:intergenic |
| ATTGATTCTCTCAGGGAAGC+TGG | 0.452667 | 1.1:+80236069 | MS.gene44406:CDS |
| AAGAGAGAACAGAGGTATCA+TGG | 0.461247 | 1.1:+80236030 | MS.gene44406:CDS |
| GTTGCTGCAGCTGCAAAGAT+TGG | 0.469361 | 1.1:+80235949 | MS.gene44406:CDS |
| AGTTTCCAGGCTTGATCTCA+AGG | 0.483054 | 1.1:-80235917 | None:intergenic |
| TGGTTATCCCACCTCCAGTC+AGG | 0.492573 | 1.1:+80235764 | MS.gene44406:CDS |
| TTCAGATATTGTGTAAGTCT+GGG | 0.506970 | 1.1:-80235790 | None:intergenic |
| TGCCTCTTCTGCAAGCTCAC+AGG | 0.512391 | 1.1:+80235888 | MS.gene44406:CDS |
| GAAGAGGCAACGGTAGCAGT+TGG | 0.531232 | 1.1:-80235874 | None:intergenic |
| GATCAAGCCTGGAAACTACT+CGG | 0.540330 | 1.1:+80235923 | MS.gene44406:CDS |
| AGCGGAACGCCTTTCGCTCA+AGG | 0.547714 | 1.1:+80235981 | MS.gene44406:CDS |
| CAGGAATGTCCTTGAGCGAA+AGG | 0.550217 | 1.1:-80235990 | None:intergenic |
| TTGCTGCAGCTGCAAAGATT+GGG | 0.550997 | 1.1:+80235950 | MS.gene44406:CDS |
| AGCGGTTATTGATTCTCTCA+GGG | 0.559363 | 1.1:+80236062 | MS.gene44406:CDS |
| TTCAAGTGAACAGAAACAGC+AGG | 0.564698 | 1.1:-80236009 | None:intergenic |
| TGGGAGGCCTGACTGGAGGT+GGG | 0.577993 | 1.1:-80235771 | None:intergenic |
| AGAACAGAGGTATCATGGAA+AGG | 0.588862 | 1.1:+80236035 | MS.gene44406:CDS |
| ATCAAGCCTGGAAACTACTC+GGG | 0.593151 | 1.1:+80235924 | MS.gene44406:CDS |
| AGTCTGGGAGGCCTGACTGG+AGG | 0.599060 | 1.1:-80235775 | None:intergenic |
| CAACGGTAGCAGTTGGAGTG+TGG | 0.604975 | 1.1:-80235867 | None:intergenic |
| TGAGCTTGCAGAAGAGGCAA+CGG | 0.638179 | 1.1:-80235884 | None:intergenic |
| AGATATTGTGTAAGTCTGGG+AGG | 0.649085 | 1.1:-80235787 | None:intergenic |
| GTATCATGGAAAGGTAAAAG+CGG | 0.664247 | 1.1:+80236044 | MS.gene44406:CDS |
| AAAGATTGGGAAGCTACTAG+CGG | 0.716694 | 1.1:+80235963 | MS.gene44406:CDS |
| TTCACTTGAAGAGAGAACAG+AGG | 0.737493 | 1.1:+80236022 | MS.gene44406:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATGCATCTTAAGAACATA+AGG | - | chr1.1:80235817-80235836 | None:intergenic | 25.0% |
| TTCAGATATTGTGTAAGTCT+GGG | - | chr1.1:80235793-80235812 | None:intergenic | 30.0% | |
| TTTCAGATATTGTGTAAGTC+TGG | - | chr1.1:80235794-80235813 | None:intergenic | 30.0% | |
| GTATCATGGAAAGGTAAAAG+CGG | + | chr1.1:80236044-80236063 | MS.gene44406:CDS | 35.0% | |
| AAAGATTGGGAAGCTACTAG+CGG | + | chr1.1:80235963-80235982 | MS.gene44406:CDS | 40.0% | |
| AGATATTGTGTAAGTCTGGG+AGG | - | chr1.1:80235790-80235809 | None:intergenic | 40.0% | |
| TTCAAGTGAACAGAAACAGC+AGG | - | chr1.1:80236012-80236031 | None:intergenic | 40.0% | |
| TTCACTTGAAGAGAGAACAG+AGG | + | chr1.1:80236022-80236041 | MS.gene44406:CDS | 40.0% | |
| !! | AAGAGAGAACAGAGGTATCA+TGG | + | chr1.1:80236030-80236049 | MS.gene44406:CDS | 40.0% |
| !! | AAGCGGTTATTGATTCTCTC+AGG | + | chr1.1:80236061-80236080 | MS.gene44406:CDS | 40.0% |
| !! | AGAACAGAGGTATCATGGAA+AGG | + | chr1.1:80236035-80236054 | MS.gene44406:CDS | 40.0% |
| !! | AGCGGTTATTGATTCTCTCA+GGG | + | chr1.1:80236062-80236081 | MS.gene44406:CDS | 40.0% |
| AGTTTCCAGGCTTGATCTCA+AGG | - | chr1.1:80235920-80235939 | None:intergenic | 45.0% | |
| ATCAAGCCTGGAAACTACTC+GGG | + | chr1.1:80235924-80235943 | MS.gene44406:CDS | 45.0% | |
| GATCAAGCCTGGAAACTACT+CGG | + | chr1.1:80235923-80235942 | MS.gene44406:CDS | 45.0% | |
| TTGCTGCAGCTGCAAAGATT+GGG | + | chr1.1:80235950-80235969 | MS.gene44406:CDS | 45.0% | |
| ! | ATTGATTCTCTCAGGGAAGC+TGG | + | chr1.1:80236069-80236088 | MS.gene44406:CDS | 45.0% |
| CAACATCCCGAGTAGTTTCC+AGG | - | chr1.1:80235933-80235952 | None:intergenic | 50.0% | |
| CAGGAATGTCCTTGAGCGAA+AGG | - | chr1.1:80235993-80236012 | None:intergenic | 50.0% | |
| GAAAGCCTTGAGATCAAGCC+TGG | + | chr1.1:80235912-80235931 | MS.gene44406:CDS | 50.0% | |
| GTTGCTGCAGCTGCAAAGAT+TGG | + | chr1.1:80235949-80235968 | MS.gene44406:CDS | 50.0% | |
| TGAGCTTGCAGAAGAGGCAA+CGG | - | chr1.1:80235887-80235906 | None:intergenic | 50.0% | |
| CAACGGTAGCAGTTGGAGTG+TGG | - | chr1.1:80235870-80235889 | None:intergenic | 55.0% | |
| CTCCTGTGAGCTTGCAGAAG+AGG | - | chr1.1:80235893-80235912 | None:intergenic | 55.0% | |
| GAAGAGGCAACGGTAGCAGT+TGG | - | chr1.1:80235877-80235896 | None:intergenic | 55.0% | |
| TGCCTCTTCTGCAAGCTCAC+AGG | + | chr1.1:80235888-80235907 | MS.gene44406:CDS | 55.0% | |
| TGGTTATCCCACCTCCAGTC+AGG | + | chr1.1:80235764-80235783 | MS.gene44406:CDS | 55.0% | |
| AGCGGAACGCCTTTCGCTCA+AGG | + | chr1.1:80235981-80236000 | MS.gene44406:CDS | 60.0% | |
| GTAAGTCTGGGAGGCCTGAC+TGG | - | chr1.1:80235781-80235800 | None:intergenic | 60.0% | |
| AGTCTGGGAGGCCTGACTGG+AGG | - | chr1.1:80235778-80235797 | None:intergenic | 65.0% | |
| TGGGAGGCCTGACTGGAGGT+GGG | - | chr1.1:80235774-80235793 | None:intergenic | 65.0% | |
| CTGGGAGGCCTGACTGGAGG+TGG | - | chr1.1:80235775-80235794 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 80235763 | 80236107 | 80235763 | ID=MS.gene44406 |
| chr1.1 | mRNA | 80235763 | 80236107 | 80235763 | ID=MS.gene44406.t1;Parent=MS.gene44406 |
| chr1.1 | exon | 80235763 | 80236107 | 80235763 | ID=MS.gene44406.t1.exon1;Parent=MS.gene44406.t1 |
| chr1.1 | CDS | 80235763 | 80236107 | 80235763 | ID=cds.MS.gene44406.t1;Parent=MS.gene44406.t1 |
| Gene Sequence |
| Protein sequence |