AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44406


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44406.t1 MTR_1g114200 99.123 114 1 0 1 114 1 114 2.37e-78 226
MS.gene44406.t1 MTR_4g032660 33.663 101 67 0 11 111 96 196 1.00e-16 72.4
MS.gene44406.t1 MTR_5g023360 70.833 48 14 0 1 48 46 93 2.11e-16 68.9
MS.gene44406.t1 MTR_3g012540 29.126 103 73 0 11 113 80 182 3.56e-13 62.8
MS.gene44406.t1 MTR_5g023330 75.000 32 8 0 1 32 88 119 2.47e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44406.t1 AT5G27820 79.825 114 23 0 1 114 1 114 3.83e-65 192
MS.gene44406.t1 AT3G20230 35.644 101 65 0 11 111 83 183 6.97e-19 77.8
MS.gene44406.t1 AT3G45020 36.937 111 58 4 16 114 4 114 3.29e-13 61.6
MS.gene44406.t1 AT3G45020 36.937 111 58 4 16 114 4 114 3.29e-13 61.6
MS.gene44406.t1 AT3G45020 36.937 111 58 4 16 114 4 114 3.29e-13 61.6
MS.gene44406.t1 AT3G45020 36.937 111 58 4 16 114 4 114 3.29e-13 61.6
MS.gene44406.t1 AT1G08845 27.184 103 75 0 11 113 82 184 2.00e-12 60.8
MS.gene44406.t1 AT1G08845 27.184 103 75 0 11 113 82 184 2.00e-12 60.8
MS.gene44406.t1 AT1G08845 27.184 103 75 0 11 113 127 229 2.71e-12 61.2

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAGATATTGTGTAAGTC+TGG 0.313396 1.1:-80235791 None:intergenic
CAACATCCCGAGTAGTTTCC+AGG 0.336475 1.1:-80235930 None:intergenic
CTGGGAGGCCTGACTGGAGG+TGG 0.388017 1.1:-80235772 None:intergenic
AAGCGGTTATTGATTCTCTC+AGG 0.400454 1.1:+80236061 MS.gene44406:CDS
GTAAGTCTGGGAGGCCTGAC+TGG 0.402204 1.1:-80235778 None:intergenic
AAATGCATCTTAAGAACATA+AGG 0.422008 1.1:-80235814 None:intergenic
GAAAGCCTTGAGATCAAGCC+TGG 0.444737 1.1:+80235912 MS.gene44406:CDS
CTCCTGTGAGCTTGCAGAAG+AGG 0.447057 1.1:-80235890 None:intergenic
ATTGATTCTCTCAGGGAAGC+TGG 0.452667 1.1:+80236069 MS.gene44406:CDS
AAGAGAGAACAGAGGTATCA+TGG 0.461247 1.1:+80236030 MS.gene44406:CDS
GTTGCTGCAGCTGCAAAGAT+TGG 0.469361 1.1:+80235949 MS.gene44406:CDS
AGTTTCCAGGCTTGATCTCA+AGG 0.483054 1.1:-80235917 None:intergenic
TGGTTATCCCACCTCCAGTC+AGG 0.492573 1.1:+80235764 MS.gene44406:CDS
TTCAGATATTGTGTAAGTCT+GGG 0.506970 1.1:-80235790 None:intergenic
TGCCTCTTCTGCAAGCTCAC+AGG 0.512391 1.1:+80235888 MS.gene44406:CDS
GAAGAGGCAACGGTAGCAGT+TGG 0.531232 1.1:-80235874 None:intergenic
GATCAAGCCTGGAAACTACT+CGG 0.540330 1.1:+80235923 MS.gene44406:CDS
AGCGGAACGCCTTTCGCTCA+AGG 0.547714 1.1:+80235981 MS.gene44406:CDS
CAGGAATGTCCTTGAGCGAA+AGG 0.550217 1.1:-80235990 None:intergenic
TTGCTGCAGCTGCAAAGATT+GGG 0.550997 1.1:+80235950 MS.gene44406:CDS
AGCGGTTATTGATTCTCTCA+GGG 0.559363 1.1:+80236062 MS.gene44406:CDS
TTCAAGTGAACAGAAACAGC+AGG 0.564698 1.1:-80236009 None:intergenic
TGGGAGGCCTGACTGGAGGT+GGG 0.577993 1.1:-80235771 None:intergenic
AGAACAGAGGTATCATGGAA+AGG 0.588862 1.1:+80236035 MS.gene44406:CDS
ATCAAGCCTGGAAACTACTC+GGG 0.593151 1.1:+80235924 MS.gene44406:CDS
AGTCTGGGAGGCCTGACTGG+AGG 0.599060 1.1:-80235775 None:intergenic
CAACGGTAGCAGTTGGAGTG+TGG 0.604975 1.1:-80235867 None:intergenic
TGAGCTTGCAGAAGAGGCAA+CGG 0.638179 1.1:-80235884 None:intergenic
AGATATTGTGTAAGTCTGGG+AGG 0.649085 1.1:-80235787 None:intergenic
GTATCATGGAAAGGTAAAAG+CGG 0.664247 1.1:+80236044 MS.gene44406:CDS
AAAGATTGGGAAGCTACTAG+CGG 0.716694 1.1:+80235963 MS.gene44406:CDS
TTCACTTGAAGAGAGAACAG+AGG 0.737493 1.1:+80236022 MS.gene44406:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAATGCATCTTAAGAACATA+AGG - chr1.1:80235817-80235836 None:intergenic 25.0%
TTCAGATATTGTGTAAGTCT+GGG - chr1.1:80235793-80235812 None:intergenic 30.0%
TTTCAGATATTGTGTAAGTC+TGG - chr1.1:80235794-80235813 None:intergenic 30.0%
GTATCATGGAAAGGTAAAAG+CGG + chr1.1:80236044-80236063 MS.gene44406:CDS 35.0%
AAAGATTGGGAAGCTACTAG+CGG + chr1.1:80235963-80235982 MS.gene44406:CDS 40.0%
AGATATTGTGTAAGTCTGGG+AGG - chr1.1:80235790-80235809 None:intergenic 40.0%
TTCAAGTGAACAGAAACAGC+AGG - chr1.1:80236012-80236031 None:intergenic 40.0%
TTCACTTGAAGAGAGAACAG+AGG + chr1.1:80236022-80236041 MS.gene44406:CDS 40.0%
!! AAGAGAGAACAGAGGTATCA+TGG + chr1.1:80236030-80236049 MS.gene44406:CDS 40.0%
!! AAGCGGTTATTGATTCTCTC+AGG + chr1.1:80236061-80236080 MS.gene44406:CDS 40.0%
!! AGAACAGAGGTATCATGGAA+AGG + chr1.1:80236035-80236054 MS.gene44406:CDS 40.0%
!! AGCGGTTATTGATTCTCTCA+GGG + chr1.1:80236062-80236081 MS.gene44406:CDS 40.0%
AGTTTCCAGGCTTGATCTCA+AGG - chr1.1:80235920-80235939 None:intergenic 45.0%
ATCAAGCCTGGAAACTACTC+GGG + chr1.1:80235924-80235943 MS.gene44406:CDS 45.0%
GATCAAGCCTGGAAACTACT+CGG + chr1.1:80235923-80235942 MS.gene44406:CDS 45.0%
TTGCTGCAGCTGCAAAGATT+GGG + chr1.1:80235950-80235969 MS.gene44406:CDS 45.0%
! ATTGATTCTCTCAGGGAAGC+TGG + chr1.1:80236069-80236088 MS.gene44406:CDS 45.0%
CAACATCCCGAGTAGTTTCC+AGG - chr1.1:80235933-80235952 None:intergenic 50.0%
CAGGAATGTCCTTGAGCGAA+AGG - chr1.1:80235993-80236012 None:intergenic 50.0%
GAAAGCCTTGAGATCAAGCC+TGG + chr1.1:80235912-80235931 MS.gene44406:CDS 50.0%
GTTGCTGCAGCTGCAAAGAT+TGG + chr1.1:80235949-80235968 MS.gene44406:CDS 50.0%
TGAGCTTGCAGAAGAGGCAA+CGG - chr1.1:80235887-80235906 None:intergenic 50.0%
CAACGGTAGCAGTTGGAGTG+TGG - chr1.1:80235870-80235889 None:intergenic 55.0%
CTCCTGTGAGCTTGCAGAAG+AGG - chr1.1:80235893-80235912 None:intergenic 55.0%
GAAGAGGCAACGGTAGCAGT+TGG - chr1.1:80235877-80235896 None:intergenic 55.0%
TGCCTCTTCTGCAAGCTCAC+AGG + chr1.1:80235888-80235907 MS.gene44406:CDS 55.0%
TGGTTATCCCACCTCCAGTC+AGG + chr1.1:80235764-80235783 MS.gene44406:CDS 55.0%
AGCGGAACGCCTTTCGCTCA+AGG + chr1.1:80235981-80236000 MS.gene44406:CDS 60.0%
GTAAGTCTGGGAGGCCTGAC+TGG - chr1.1:80235781-80235800 None:intergenic 60.0%
AGTCTGGGAGGCCTGACTGG+AGG - chr1.1:80235778-80235797 None:intergenic 65.0%
TGGGAGGCCTGACTGGAGGT+GGG - chr1.1:80235774-80235793 None:intergenic 65.0%
CTGGGAGGCCTGACTGGAGG+TGG - chr1.1:80235775-80235794 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 80235763 80236107 80235763 ID=MS.gene44406
chr1.1 mRNA 80235763 80236107 80235763 ID=MS.gene44406.t1;Parent=MS.gene44406
chr1.1 exon 80235763 80236107 80235763 ID=MS.gene44406.t1.exon1;Parent=MS.gene44406.t1
chr1.1 CDS 80235763 80236107 80235763 ID=cds.MS.gene44406.t1;Parent=MS.gene44406.t1
Gene Sequence

>MS.gene44406

ATGGTTATCCCACCTCCAGTCAGGCCTCCCAGACTTACACAATATCTGAAACCTTATGTTCTTAAGATGCATTTCACAAATAAGTATGTGAGTGCTCAAGTGATCCACACTCCAACTGCTACCGTTGCCTCTTCTGCAAGCTCACAGGAGAAAGCCTTGAGATCAAGCCTGGAAACTACTCGGGATGTTGCTGCAGCTGCAAAGATTGGGAAGCTACTAGCGGAACGCCTTTCGCTCAAGGACATTCCTGCTGTTTCTGTTCACTTGAAGAGAGAACAGAGGTATCATGGAAAGGTAAAAGCGGTTATTGATTCTCTCAGGGAAGCTGGTGTTAAGTTGCTTTGA

Protein sequence

>MS.gene44406.t1

MVIPPPVRPPRLTQYLKPYVLKMHFTNKYVSAQVIHTPTATVASSASSQEKALRSSLETTRDVAAAAKIGKLLAERLSLKDIPAVSVHLKREQRYHGKVKAVIDSLREAGVKLL