AlfalfaGEDB | MS.gene45147

M. sativa cultivar XinJiangDaYe / MS.gene45147

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0016787 GO:0019538 GO:0031012 GO:0043167 GO:0043169 GO:0043170 GO:0044238 GO:0046872 GO:0046914 GO:0070011 GO:0071704 GO:0110165 GO:0140096 GO:1901564
PFAM: PF00413.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45147.t1	XP_003613392.1	93	172	12	0	1	172	151	322	9.20E-83	316.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45147.t1	O04529	48.2	166	74	6	15	171	164	326	2.1e-27	123.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45147.t1	G7K5I7	93.0	172	12	0	1	172	151	322	6.6e-83	316.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049259	MS.gene45147	0.803829	2.89E-49	-1.69E-46
MS.gene04986	MS.gene45147	0.820267	7.51E-53	-1.69E-46
MS.gene050173	MS.gene45147	0.828544	8.48E-55	-1.69E-46
MS.gene050883	MS.gene45147	0.813897	2.03E-51	-1.69E-46
MS.gene051044	MS.gene45147	0.800252	1.57E-48	-1.69E-46
MS.gene051127	MS.gene45147	0.816674	4.90E-52	-1.69E-46
MS.gene051138	MS.gene45147	0.814233	1.71E-51	-1.69E-46
MS.gene053488	MS.gene45147	0.805726	1.16E-49	-1.69E-46
MS.gene053561	MS.gene45147	0.818068	2.38E-52	-1.69E-46
MS.gene054056	MS.gene45147	0.859	5.60E-63	-1.69E-46
MS.gene054057	MS.gene45147	0.807165	5.78E-50	-1.69E-46
MS.gene055124	MS.gene45147	0.828692	7.81E-55	-1.69E-46
MS.gene055942	MS.gene45147	0.825432	4.70E-54	-1.69E-46
MS.gene056311	MS.gene45147	0.802709	4.93E-49	-1.69E-46
MS.gene058099	MS.gene45147	0.805884	1.08E-49	-1.69E-46
MS.gene058108	MS.gene45147	0.818698	1.71E-52	-1.69E-46
MS.gene058109	MS.gene45147	0.848839	4.74E-60	-1.69E-46
MS.gene058111	MS.gene45147	0.809759	1.61E-50	-1.69E-46
MS.gene058171	MS.gene45147	0.843996	9.94E-59	-1.69E-46
MS.gene058173	MS.gene45147	0.836469	9.19E-57	-1.69E-46
MS.gene058175	MS.gene45147	0.848743	5.05E-60	-1.69E-46
MS.gene058493	MS.gene45147	0.824032	1.01E-53	-1.69E-46
MS.gene058628	MS.gene45147	0.810074	1.38E-50	-1.69E-46
MS.gene059690	MS.gene45147	0.83349	5.17E-56	-1.69E-46
MS.gene059694	MS.gene45147	0.824324	8.58E-54	-1.69E-46
MS.gene059697	MS.gene45147	0.816529	5.28E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45147.t1	MTR_5g036080	93.023	172	12	0	1	172	151	322	3.49e-114	327
MS.gene45147.t1	MTR_8g062770	44.910	167	82	5	13	171	164	328	2.18e-35	127
MS.gene45147.t1	MTR_5g036410	45.238	168	80	7	13	171	164	328	1.29e-34	125
MS.gene45147.t1	MTR_5g036087	38.554	166	88	5	16	168	148	312	8.84e-31	114
MS.gene45147.t1	MTR_5g036290	38.323	167	88	6	16	172	172	333	1.32e-30	114
MS.gene45147.t1	MTR_5g036270	37.725	167	89	5	16	168	139	304	5.66e-29	109
MS.gene45147.t1	MTR_5g036360	36.066	183	101	7	1	172	146	323	1.04e-27	106
MS.gene45147.t1	MTR_8g005900	35.119	168	100	3	13	171	142	309	2.74e-27	105
MS.gene45147.t1	MTR_5g036093	33.918	171	98	5	16	172	119	288	5.85e-27	103
MS.gene45147.t1	MTR_5g012680	36.095	169	97	5	13	171	148	315	5.22e-26	102
MS.gene45147.t1	MTR_5g036083	34.969	163	86	6	16	172	129	277	4.04e-22	90.9
MS.gene45147.t1	MTR_5g036310	37.190	121	64	4	59	168	157	276	4.13e-18	80.1
MS.gene45147.t1	MTR_5g036365	30.833	120	73	3	16	125	43	162	8.19e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45147.t1	AT1G70170	48.193	166	74	6	15	171	164	326	4.52e-37	131
MS.gene45147.t1	AT1G59970	40.588	170	82	6	13	168	149	313	3.41e-32	118
MS.gene45147.t1	AT1G24140	39.444	180	88	5	8	171	157	331	5.18e-32	118
MS.gene45147.t1	AT2G45040	36.310	168	98	3	13	171	131	298	7.65e-27	103
MS.gene45147.t1	AT4G16640	35.602	191	95	9	1	171	139	321	5.16e-25	99.4

Find 34 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGAGGATTCAGGAAATTTA+TGG	0.195702	5.1:+26752924	MS.gene45147:CDS
ATTGAGACAACGTCGTTTAA+TGG	0.197767	5.1:+26752612	MS.gene45147:CDS
TGATGATTTGCAGAGGATTC+AGG	0.285562	5.1:+26752914	MS.gene45147:CDS
CATCAAGTTGGTCATTTACT+AGG	0.286156	5.1:+26752813	MS.gene45147:CDS
GTTGGTCATTTACTAGGGTT+GGG	0.351181	5.1:+26752819	MS.gene45147:CDS
GTTGGTAAAATTGTTCTTCA+TGG	0.354896	5.1:-26752439	None:intergenic
TGAAGAGGATGATGTGGATT+TGG	0.356149	5.1:+26752776	MS.gene45147:CDS
TAATGTTGGCAGTGTATACT+TGG	0.438145	5.1:+26752713	MS.gene45147:CDS
AGTTGGTCATTTACTAGGGT+TGG	0.439176	5.1:+26752818	MS.gene45147:CDS
GATGGAAAAGGTGGAACGGT+TGG	0.463287	5.1:+26752666	MS.gene45147:CDS
TTGTATGTGACATTATTCTC+TGG	0.465314	5.1:-26752528	None:intergenic
GGAAAAGGTGGAACGGTTGG+AGG	0.467603	5.1:+26752669	MS.gene45147:CDS
GTTGGGGCATTCTTCTGTTG+AGG	0.484015	5.1:+26752836	MS.gene45147:CDS
GGGGCATTCTTCTGTTGAGG+AGG	0.515125	5.1:+26752839	MS.gene45147:CDS
CTTGGATGCAGAGGAACAAT+GGG	0.516411	5.1:+26752731	MS.gene45147:CDS
ATCAAGTTGGTCATTTACTA+GGG	0.524382	5.1:+26752814	MS.gene45147:CDS
TTCAAGACGCGTTTAACAGT+TGG	0.530967	5.1:+26752565	MS.gene45147:CDS
TTGGTCATTTACTAGGGTTG+GGG	0.543515	5.1:+26752820	MS.gene45147:CDS
ACTTGGATGCAGAGGAACAA+TGG	0.545642	5.1:+26752730	MS.gene45147:CDS
TTCTCCACGTTCCTCCACCA+CGG	0.547332	5.1:-26752480	None:intergenic
ATAAACAATAGTGTTAATGT+TGG	0.559196	5.1:+26752699	MS.gene45147:CDS
TTGACGTTGGATGGAAAAGG+TGG	0.581789	5.1:+26752657	MS.gene45147:CDS
GCAAGAAAAGAAGATTGAGT+TGG	0.588378	5.1:+26752884	MS.gene45147:CDS
TGTTGTTGAAGAGGATGATG+TGG	0.588380	5.1:+26752770	MS.gene45147:CDS
TGTATGTGACATTATTCTCT+GGG	0.592673	5.1:-26752527	None:intergenic
TAGTGAAAATGTTGTTGAAG+AGG	0.596863	5.1:+26752761	MS.gene45147:CDS
CGTCGTTTAAACCGTGGTGG+AGG	0.600911	5.1:+26752469	MS.gene45147:CDS
TAAACCGTGGTGGAGGAACG+TGG	0.611698	5.1:+26752476	MS.gene45147:CDS
CAGTGTATACTTGGATGCAG+AGG	0.637866	5.1:+26752722	MS.gene45147:CDS
TCTGTTGTGATGCATCAAGT+TGG	0.649877	5.1:+26752801	MS.gene45147:CDS
CAGCGTCGTTTAAACCGTGG+TGG	0.668848	5.1:+26752466	MS.gene45147:CDS
TGAATGATGATGATTTGCAG+AGG	0.675761	5.1:+26752907	MS.gene45147:CDS
GTTGGATGGAAAAGGTGGAA+CGG	0.714060	5.1:+26752662	MS.gene45147:CDS
AAACAGCGTCGTTTAAACCG+TGG	0.715782	5.1:+26752463	MS.gene45147:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAACAATAGTGTTAATGT+TGG	+	chr5.1:26752699-26752718	MS.gene45147:CDS	20.0%
	TAGTGAAAATGTTGTTGAAG+AGG	+	chr5.1:26752761-26752780	MS.gene45147:CDS	30.0%
	TGTATGTGACATTATTCTCT+GGG	-	chr5.1:26752530-26752549	None:intergenic	30.0%
	TTGTATGTGACATTATTCTC+TGG	-	chr5.1:26752531-26752550	None:intergenic	30.0%
!	TCTTCAACAACATTTTCACT+AGG	-	chr5.1:26752762-26752781	None:intergenic	30.0%
!	TTCTTTTCTTGCAACACAAT+CGG	-	chr5.1:26752876-26752895	None:intergenic	30.0%
!!	ATCAAGTTGGTCATTTACTA+GGG	+	chr5.1:26752814-26752833	MS.gene45147:CDS	30.0%
	ATTGAGACAACGTCGTTTAA+TGG	+	chr5.1:26752612-26752631	MS.gene45147:CDS	35.0%
	CAGAGGATTCAGGAAATTTA+TGG	+	chr5.1:26752924-26752943	MS.gene45147:CDS	35.0%
	GCAAGAAAAGAAGATTGAGT+TGG	+	chr5.1:26752884-26752903	MS.gene45147:CDS	35.0%
	TAATGTTGGCAGTGTATACT+TGG	+	chr5.1:26752713-26752732	MS.gene45147:CDS	35.0%
!	TGAATGATGATGATTTGCAG+AGG	+	chr5.1:26752907-26752926	MS.gene45147:CDS	35.0%
!	TTTTTGACGTTGGATGGAAA+AGG	+	chr5.1:26752654-26752673	MS.gene45147:CDS	35.0%
!!	CATCAAGTTGGTCATTTACT+AGG	+	chr5.1:26752813-26752832	MS.gene45147:CDS	35.0%
!!	TTTAAACGACGCTGTTTTGT+TGG	-	chr5.1:26752460-26752479	None:intergenic	35.0%
	AGTTGGTCATTTACTAGGGT+TGG	+	chr5.1:26752818-26752837	MS.gene45147:CDS	40.0%
	GTTGGTCATTTACTAGGGTT+GGG	+	chr5.1:26752819-26752838	MS.gene45147:CDS	40.0%
	TCTGTTGTGATGCATCAAGT+TGG	+	chr5.1:26752801-26752820	MS.gene45147:CDS	40.0%
	TGAAGAGGATGATGTGGATT+TGG	+	chr5.1:26752776-26752795	MS.gene45147:CDS	40.0%
	TGTTGTTGAAGAGGATGATG+TGG	+	chr5.1:26752770-26752789	MS.gene45147:CDS	40.0%
	TTCAAGACGCGTTTAACAGT+TGG	+	chr5.1:26752565-26752584	MS.gene45147:CDS	40.0%
	TTGGTCATTTACTAGGGTTG+GGG	+	chr5.1:26752820-26752839	MS.gene45147:CDS	40.0%
!	ATAGCGTTTTTGACGTTGGA+TGG	+	chr5.1:26752648-26752667	MS.gene45147:CDS	40.0%
!	TCGTATAGCGTTTTTGACGT+TGG	+	chr5.1:26752644-26752663	MS.gene45147:CDS	40.0%
!	TGATGATTTGCAGAGGATTC+AGG	+	chr5.1:26752914-26752933	MS.gene45147:CDS	40.0%
	AAACAGCGTCGTTTAAACCG+TGG	+	chr5.1:26752463-26752482	MS.gene45147:CDS	45.0%
	ACTTGGATGCAGAGGAACAA+TGG	+	chr5.1:26752730-26752749	MS.gene45147:CDS	45.0%
	CAGTGTATACTTGGATGCAG+AGG	+	chr5.1:26752722-26752741	MS.gene45147:CDS	45.0%
	CTTGGATGCAGAGGAACAAT+GGG	+	chr5.1:26752731-26752750	MS.gene45147:CDS	45.0%
	GTTGGATGGAAAAGGTGGAA+CGG	+	chr5.1:26752662-26752681	MS.gene45147:CDS	45.0%
	TTGACGTTGGATGGAAAAGG+TGG	+	chr5.1:26752657-26752676	MS.gene45147:CDS	45.0%
	GATGGAAAAGGTGGAACGGT+TGG	+	chr5.1:26752666-26752685	MS.gene45147:CDS	50.0%
!	GTTGGGGCATTCTTCTGTTG+AGG	+	chr5.1:26752836-26752855	MS.gene45147:CDS	50.0%
	CAGCGTCGTTTAAACCGTGG+TGG	+	chr5.1:26752466-26752485	MS.gene45147:CDS	55.0%
	CGTCGTTTAAACCGTGGTGG+AGG	+	chr5.1:26752469-26752488	MS.gene45147:CDS	55.0%
	GGAAAAGGTGGAACGGTTGG+AGG	+	chr5.1:26752669-26752688	MS.gene45147:CDS	55.0%
	TTCTCCACGTTCCTCCACCA+CGG	-	chr5.1:26752483-26752502	None:intergenic	55.0%
!	GGGGCATTCTTCTGTTGAGG+AGG	+	chr5.1:26752839-26752858	MS.gene45147:CDS	55.0%
!	TAAACCGTGGTGGAGGAACG+TGG	+	chr5.1:26752476-26752495	MS.gene45147:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	26752441	26752959	26752441	ID=MS.gene45147
chr5.1	mRNA	26752441	26752959	26752441	ID=MS.gene45147.t1;Parent=MS.gene45147
chr5.1	exon	26752441	26752959	26752441	ID=MS.gene45147.t1.exon1;Parent=MS.gene45147.t1
chr5.1	CDS	26752441	26752959	26752441	ID=cds.MS.gene45147.t1;Parent=MS.gene45147.t1

Gene Sequence

>MS.gene45147

ATGAAGAACAATTTTACCAACAAAACAGCGTCGTTTAAACCGTGGTGGAGGAACGTGGAGAACAGATCGTTAGCATATGCTTTTCACCCAGAGAATAATGTCACATACAATGTTAAGTCTTTGTTTCAAGACGCGTTTAACAGTTGGTCAAATGCAACTGCATTGAACTTCATTGAGACAACGTCGTTTAATGGTTCAGATATTCGTATAGCGTTTTTGACGTTGGATGGAAAAGGTGGAACGGTTGGAGGTAGTAATATAAACAATAGTGTTAATGTTGGCAGTGTATACTTGGATGCAGAGGAACAATGGGTGTTACCTAGTGAAAATGTTGTTGAAGAGGATGATGTGGATTTGGAATCTGTTGTGATGCATCAAGTTGGTCATTTACTAGGGTTGGGGCATTCTTCTGTTGAGGAGGCTATTATGTATCCGATTGTGTTGCAAGAAAAGAAGATTGAGTTGGTGAATGATGATGATTTGCAGAGGATTCAGGAAATTTATGGTGTCAATACTTGA

Protein sequence

>MS.gene45147.t1

MKNNFTNKTASFKPWWRNVENRSLAYAFHPENNVTYNVKSLFQDAFNSWSNATALNFIETTSFNGSDIRIAFLTLDGKGGTVGGSNINNSVNVGSVYLDAEEQWVLPSENVVEEDDVDLESVVMHQVGHLLGLGHSSVEEAIMYPIVLQEKKIELVNDDDLQRIQEIYGVNT