AlfalfaGEDB | MS.gene45274

M. sativa cultivar XinJiangDaYe / MS.gene45274

KEGG: K00276
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005507 GO:0006807 GO:0008131 GO:0008150 GO:0008152 GO:0009308 GO:0016491 GO:0016638 GO:0016641 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0048037 GO:0048038 GO:0055114 GO:0071704 GO:1901564
PFAM: PF02728.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45274.t1	RHN60225.1	95.7	138	6	0	1	138	126	263	3.50E-69	270.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45274.t1	Q43077	77.4	137	31	0	1	137	127	263	2.2e-59	229.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45274.t1	A0A396I997	95.7	138	6	0	1	138	126	263	2.6e-69	270.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene45274	0.819281	1.26E-52	-1.69E-46
MS.gene049217	MS.gene45274	0.819107	1.38E-52	-1.69E-46
MS.gene049222	MS.gene45274	0.814044	1.88E-51	-1.69E-46
MS.gene049285	MS.gene45274	0.834536	2.83E-56	-1.69E-46
MS.gene049320	MS.gene45274	0.813219	2.86E-51	-1.69E-46
MS.gene050504	MS.gene45274	0.817641	2.97E-52	-1.69E-46
MS.gene050699	MS.gene45274	0.822379	2.44E-53	-1.69E-46
MS.gene050772	MS.gene45274	0.816144	6.43E-52	-1.69E-46
MS.gene051551	MS.gene45274	0.802483	5.49E-49	-1.69E-46
MS.gene052503	MS.gene45274	0.837715	4.41E-57	-1.69E-46
MS.gene052823	MS.gene45274	0.832093	1.15E-55	-1.69E-46
MS.gene052825	MS.gene45274	0.833022	6.77E-56	-1.69E-46
MS.gene053213	MS.gene45274	0.831347	1.76E-55	-1.69E-46
MS.gene053286	MS.gene45274	0.828227	1.01E-54	-1.69E-46
MS.gene053480	MS.gene45274	0.813008	3.18E-51	-1.69E-46
MS.gene053632	MS.gene45274	0.81787	2.63E-52	-1.69E-46
MS.gene054595	MS.gene45274	0.801252	9.83E-49	-1.69E-46
MS.gene054642	MS.gene45274	0.813903	2.02E-51	-1.69E-46
MS.gene054954	MS.gene45274	0.818782	1.64E-52	-1.69E-46
MS.gene055362	MS.gene45274	0.829156	6.03E-55	-1.69E-46
MS.gene055502	MS.gene45274	0.832813	7.63E-56	-1.69E-46
MS.gene055722	MS.gene45274	0.810111	1.36E-50	-1.69E-46
MS.gene056374	MS.gene45274	0.811293	7.53E-51	-1.69E-46
MS.gene056510	MS.gene45274	0.823668	1.22E-53	-1.69E-46
MS.gene056657	MS.gene45274	0.826052	3.35E-54	-1.69E-46
MS.gene057416	MS.gene45274	0.823832	1.12E-53	-1.69E-46
MS.gene058419	MS.gene45274	0.829463	5.08E-55	-1.69E-46
MS.gene059022	MS.gene45274	0.814737	1.32E-51	-1.69E-46
MS.gene059677	MS.gene45274	0.818295	2.11E-52	-1.69E-46
MS.gene059718	MS.gene45274	0.81805	2.40E-52	-1.69E-46
MS.gene059756	MS.gene45274	0.814462	1.52E-51	-1.69E-46
MS.gene06077	MS.gene45274	0.810145	1.33E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45274.t1	MTR_4g045887	95.620	137	6	0	1	137	126	262	1.35e-90	276
MS.gene45274.t1	MTR_4g117610	81.022	137	26	0	1	137	121	257	2.98e-76	238
MS.gene45274.t1	MTR_4g117670	79.412	136	28	0	2	137	45	180	3.83e-76	236
MS.gene45274.t1	MTR_4g117660	75.735	136	33	0	2	137	129	264	2.08e-74	234
MS.gene45274.t1	MTR_1g104590	53.285	137	64	0	1	137	125	261	7.75e-47	160
MS.gene45274.t1	MTR_1g104550	40.310	129	71	1	10	138	140	262	3.50e-31	117
MS.gene45274.t1	MTR_3g080500	32.886	149	80	4	2	138	173	313	1.51e-24	98.2
MS.gene45274.t1	MTR_4g098910	37.963	108	16	1	2	109	91	147	2.28e-14	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene45274.t1	AT1G31710	47.143	140	72	1	1	138	125	264	2.85e-40	142
MS.gene45274.t1	AT1G31690	47.826	138	72	0	1	138	124	261	2.35e-38	137
MS.gene45274.t1	AT1G31670	42.029	138	80	0	1	138	204	341	2.96e-35	128
MS.gene45274.t1	AT4G14940	41.346	104	61	0	10	113	129	232	5.89e-28	107
MS.gene45274.t1	AT4G12290	35.616	146	82	5	1	138	176	317	1.61e-25	100
MS.gene45274.t1	AT4G12290	35.616	146	82	5	1	138	176	317	1.71e-25	100
MS.gene45274.t1	AT1G62810	36.620	142	85	5	1	138	153	293	2.69e-24	97.4
MS.gene45274.t1	AT4G12270	34.266	143	89	4	1	138	178	320	4.34e-24	96.3
MS.gene45274.t1	AT3G43670	31.690	142	92	5	1	138	134	274	2.96e-20	85.9

Find 27 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGAGAAGAAGAGAGTAAA+AGG	0.281690	4.2:-54734517	MS.gene45274:CDS
CTTGCAAGACTATGCTGTTT+TGG	0.293628	4.2:+54734305	None:intergenic
CCATTCTCCATGGGATGGTT+TGG	0.335288	4.2:-54734536	MS.gene45274:CDS
TGATCAATGGCCATAGTGTT+AGG	0.343874	4.2:-54734256	MS.gene45274:CDS
GGCTGAGAACACTGAATATA+AGG	0.350226	4.2:-54734354	MS.gene45274:CDS
ACCATTGTTAATCCACTAAT+AGG	0.372587	4.2:+54734440	None:intergenic
TCAAAACAAAACCCACCATT+TGG	0.375819	4.2:-54734329	MS.gene45274:CDS
ACATTACTTACCTAACACTA+TGG	0.401614	4.2:+54734246	None:intergenic
CCAAACCATCCCATGGAGAA+TGG	0.469490	4.2:+54734536	None:intergenic
ATATTTCAATGGAAGCTTTG+TGG	0.478356	4.2:+54734619	None:intergenic
GGTCCTGGCTTCTTGATCAA+TGG	0.482633	4.2:-54734269	MS.gene45274:CDS
AGTCATCAACCTCATGGTCC+TGG	0.502438	4.2:-54734284	MS.gene45274:CDS
ATGAAAGGAGGATATTTCAA+TGG	0.522174	4.2:+54734608	None:intergenic
GTGCTCCATTCTCCATGGGA+TGG	0.547411	4.2:-54734541	MS.gene45274:CDS
TTCTTCTCCAAACCATCCCA+TGG	0.551724	4.2:+54734529	None:intergenic
GGTGTGTGCTCCATTCTCCA+TGG	0.556455	4.2:-54734546	MS.gene45274:CDS
TTCAGTGAAGCAATGAAAGG+AGG	0.577218	4.2:+54734596	None:intergenic
AGAAATTGAAGTTGTGCCTA+CGG	0.578180	4.2:-54734375	MS.gene45274:CDS
GTGTGTGCTCCATTCTCCAT+GGG	0.582146	4.2:-54734545	MS.gene45274:CDS
CTTGCAAGTCATCAACCTCA+TGG	0.583899	4.2:-54734290	MS.gene45274:CDS
TGGCCATTGATCAAGAAGCC+AGG	0.591551	4.2:+54734266	None:intergenic
AGGTTTGAATCTCTCTGAAG+TGG	0.591848	4.2:-54734567	MS.gene45274:CDS
GCCTATTAGTGGATTAACAA+TGG	0.596660	4.2:-54734441	MS.gene45274:CDS
ATGTTATCTCTTGAGGAACA+AGG	0.623725	4.2:-54734647	MS.gene45274:CDS
TATTCAGTGTTCTCAGCCGT+AGG	0.626136	4.2:+54734359	None:intergenic
TGTTCCTCAAGAGATAACAT+AGG	0.638410	4.2:+54734650	None:intergenic
ATCAAGAAGCCAGGACCATG+AGG	0.651972	4.2:+54734275	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACACTGTTAATTTTTTTGTG+AGG	-	chr4.2:54734437-54734456	MS.gene45274:CDS	25.0%
	ACCATTGTTAATCCACTAAT+AGG	+	chr4.2:54734463-54734482	None:intergenic	30.0%
	ATATTTCAATGGAAGCTTTG+TGG	+	chr4.2:54734284-54734303	None:intergenic	30.0%
	ATGAAAGGAGGATATTTCAA+TGG	+	chr4.2:54734295-54734314	None:intergenic	30.0%
!!	TTTTTCAGTGAAGCAATGAA+AGG	+	chr4.2:54734310-54734329	None:intergenic	30.0%
	AGAAATTGAAGTTGTGCCTA+CGG	-	chr4.2:54734525-54734544	MS.gene45274:CDS	35.0%
	ATGTTATCTCTTGAGGAACA+AGG	-	chr4.2:54734253-54734272	MS.gene45274:CDS	35.0%
	ATTGCTTCACTGAAAAAGAG+AGG	-	chr4.2:54734313-54734332	MS.gene45274:CDS	35.0%
	TCAAAACAAAACCCACCATT+TGG	-	chr4.2:54734571-54734590	MS.gene45274:CDS	35.0%
	TTGGAGAAGAAGAGAGTAAA+AGG	-	chr4.2:54734383-54734402	MS.gene45274:CDS	35.0%
!!	GCCTATTAGTGGATTAACAA+TGG	-	chr4.2:54734459-54734478	MS.gene45274:CDS	35.0%
!!!	TTTTTTGTGAGGCCTATTAG+TGG	-	chr4.2:54734448-54734467	MS.gene45274:CDS	35.0%
	GGCTGAGAACACTGAATATA+AGG	-	chr4.2:54734546-54734565	MS.gene45274:CDS	40.0%
	TGATCAATGGCCATAGTGTT+AGG	-	chr4.2:54734644-54734663	MS.gene45274:CDS	40.0%
	TTCAGTGAAGCAATGAAAGG+AGG	+	chr4.2:54734307-54734326	None:intergenic	40.0%
!	AGGTTTGAATCTCTCTGAAG+TGG	-	chr4.2:54734333-54734352	MS.gene45274:CDS	40.0%
!!	CTTGCAAGACTATGCTGTTT+TGG	+	chr4.2:54734598-54734617	None:intergenic	40.0%
!!!	CTATGCTGTTTTGGTCCAAA+TGG	+	chr4.2:54734589-54734608	None:intergenic	40.0%
	CTTGCAAGTCATCAACCTCA+TGG	-	chr4.2:54734610-54734629	MS.gene45274:CDS	45.0%
	TATTCAGTGTTCTCAGCCGT+AGG	+	chr4.2:54734544-54734563	None:intergenic	45.0%
	TTCTTCTCCAAACCATCCCA+TGG	+	chr4.2:54734374-54734393	None:intergenic	45.0%
!!!	GCTGTTTTGGTCCAAATGGT+GGG	+	chr4.2:54734585-54734604	None:intergenic	45.0%
!!!	TGCTGTTTTGGTCCAAATGG+TGG	+	chr4.2:54734586-54734605	None:intergenic	45.0%
	AGTCATCAACCTCATGGTCC+TGG	-	chr4.2:54734616-54734635	MS.gene45274:CDS	50.0%
	ATCAAGAAGCCAGGACCATG+AGG	+	chr4.2:54734628-54734647	None:intergenic	50.0%
	CCAAACCATCCCATGGAGAA+TGG	+	chr4.2:54734367-54734386	None:intergenic	50.0%
	CCATTCTCCATGGGATGGTT+TGG	-	chr4.2:54734364-54734383	MS.gene45274:CDS	50.0%
	GGTCCTGGCTTCTTGATCAA+TGG	-	chr4.2:54734631-54734650	MS.gene45274:CDS	50.0%
	GTGTGTGCTCCATTCTCCAT+GGG	-	chr4.2:54734355-54734374	MS.gene45274:CDS	50.0%
	TGGCCATTGATCAAGAAGCC+AGG	+	chr4.2:54734637-54734656	None:intergenic	50.0%
	GGTGTGTGCTCCATTCTCCA+TGG	-	chr4.2:54734354-54734373	MS.gene45274:CDS	55.0%
	GTGCTCCATTCTCCATGGGA+TGG	-	chr4.2:54734359-54734378	MS.gene45274:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	54734253	54734669	54734253	ID=MS.gene45274
chr4.2	mRNA	54734253	54734669	54734253	ID=MS.gene45274.t1;Parent=MS.gene45274
chr4.2	exon	54734253	54734669	54734253	ID=MS.gene45274.t1.exon1;Parent=MS.gene45274.t1
chr4.2	CDS	54734253	54734669	54734253	ID=cds.MS.gene45274.t1;Parent=MS.gene45274.t1

Gene Sequence

>MS.gene45274

ATGTTATCTCTTGAGGAACAAGGTCTTGCCACAAAGCTTCCATTGAAATATCCTCCTTTCATTGCTTCACTGAAAAAGAGAGGTTTGAATCTCTCTGAAGTGGTGTGTGCTCCATTCTCCATGGGATGGTTTGGAGAAGAAGAGAGTAAAAGGACTGTGAGAGTTGATTGTTTTATGAAAGAAAACACTGTTAATTTTTTTGTGAGGCCTATTAGTGGATTAACAATGGTTGCTGATCTTGAACTCATGAAAATTGTTGAATATCAAGATAGAGAAATTGAAGTTGTGCCTACGGCTGAGAACACTGAATATAAGGCTTCAAAACAAAACCCACCATTTGGACCAAAACAGCATAGTCTTGCAAGTCATCAACCTCATGGTCCTGGCTTCTTGATCAATGGCCATAGTGTTAGGTAA

Protein sequence

>MS.gene45274.t1

MLSLEEQGLATKLPLKYPPFIASLKKRGLNLSEVVCAPFSMGWFGEEESKRTVRVDCFMKENTVNFFVRPISGLTMVADLELMKIVEYQDREIEVVPTAENTEYKASKQNPPFGPKQHSLASHQPHGPGFLINGHSVR