AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45340


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45340.t1 MTR_4g046047 98.052 154 3 0 1 154 1 154 1.49e-111 313
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45340.t1 AT1G03650 69.079 152 44 1 6 154 6 157 2.04e-74 219

Find 31 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGTTATGTCATGTATTCT+TGG 0.193464 4.3:-54155130 MS.gene45340:CDS
AGTGGAAGAGATAGTGAAAT+TGG 0.247431 4.3:-54155258 MS.gene45340:CDS
CACGTTGATGCCCAACTTGC+AGG 0.261704 4.3:-54155149 MS.gene45340:CDS
CATGTTGACTGTTTGGTTGA+AGG 0.355783 4.3:-54153746 MS.gene45340:CDS
GAAGCGAGTGATTCGTGTTT+GGG 0.367842 4.3:+54155221 None:intergenic
GGAAGCGAGTGATTCGTGTT+TGG 0.373847 4.3:+54155220 None:intergenic
TGGCTTTCATGTTGACTGTT+TGG 0.383570 4.3:-54153753 MS.gene45340:CDS
CATGACATAACCTGCAAGTT+GGG 0.434152 4.3:+54155139 None:intergenic
TGGACTTCGATTCAAATTGA+AGG 0.474763 4.3:-54153685 None:intergenic
AAATGCTTATAGAATGTACT+TGG 0.517363 4.3:-54153705 MS.gene45340:CDS
TCTTGTACAGATTCAGCGCA+GGG 0.523854 4.3:+54153780 None:intergenic
CAATGGAGGAGGCAAGGACA+CGG 0.532439 4.3:-54153887 MS.gene45340:CDS
ACATGACATAACCTGCAAGT+TGG 0.533996 4.3:+54155138 None:intergenic
GGAGTTGGAACCTGCGAGCT+TGG 0.538376 4.3:+54155082 None:intergenic
GCAGAACAAGAAAAGTTTCG+CGG 0.541873 4.3:-54153832 MS.gene45340:CDS
GCTTCTATCACCAAGCTCGC+AGG 0.549548 4.3:-54155092 MS.gene45340:intron
TTCTTGTACAGATTCAGCGC+AGG 0.552919 4.3:+54153779 None:intergenic
AAGGAGCAATGGAGGAGGCA+AGG 0.558770 4.3:-54153893 MS.gene45340:CDS
ATAGAAGCATACAATGAAGA+AGG 0.562971 4.3:+54155107 None:intergenic
CAGAAACAGTGGTAAAGTAG+TGG 0.572015 4.3:-54155276 MS.gene45340:CDS
CGCATGAGTGATGGCAAATG+TGG 0.573974 4.3:-54155315 None:intergenic
TGCTTCACGTTGATCCGTTG+AGG 0.576802 4.3:-54153805 MS.gene45340:CDS
CTTCTGTAGTGAAGGAGCAA+TGG 0.596774 4.3:-54153904 MS.gene45340:intron
CTGAATCTGTACAAGAAGCA+TGG 0.599242 4.3:-54153773 MS.gene45340:CDS
CTGTAGTGAAGGAGCAATGG+AGG 0.602271 4.3:-54153901 MS.gene45340:intron
TAGTGAAGGAGCAATGGAGG+AGG 0.603189 4.3:-54153898 MS.gene45340:CDS
CGGAGAGGTTCTACTGAAAG+CGG 0.611656 4.3:-54153867 MS.gene45340:CDS
TTCAGCGCAGGGGTCCTCAA+CGG 0.614591 4.3:+54153791 None:intergenic
AATGAACTTGACAGAAACAG+TGG 0.667655 4.3:-54155287 MS.gene45340:CDS
CTTGTACAGATTCAGCGCAG+GGG 0.671497 4.3:+54153781 None:intergenic
GAGGAGGCAAGGACACGGAG+AGG 0.683029 4.3:-54153882 MS.gene45340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAACTATCTTTAATATA+AGG - chr4.3:54154951-54154970 MS.gene45340:intron 10.0%
!! ATTAACTATCTTTAATATAA+GGG - chr4.3:54154952-54154971 MS.gene45340:intron 10.0%
!! AAAGAAATTATCCAATTTAT+AGG - chr4.3:54154301-54154320 MS.gene45340:intron 15.0%
!! AAGAAATTATCCAATTTATA+GGG - chr4.3:54154302-54154321 MS.gene45340:intron 15.0%
!! ATAAGAAATTGCAAAAAAAT+TGG - chr4.3:54154354-54154373 MS.gene45340:intron 15.0%
!! AGAAATTATCCAATTTATAG+GGG - chr4.3:54154303-54154322 MS.gene45340:intron 20.0%
!! CAATAAAAATAATCTGTTCT+TGG + chr4.3:54154776-54154795 None:intergenic 20.0%
!! CTAAACGATTGAAATATAAT+AGG + chr4.3:54154502-54154521 None:intergenic 20.0%
!! TTATATTAACCCCTATAAAT+TGG + chr4.3:54154315-54154334 None:intergenic 20.0%
!!! ATTAATTTGGAAAAACAGTA+GGG - chr4.3:54154746-54154765 MS.gene45340:intron 20.0%
!!! CAAGAACAGATTATTTTTAT+TGG - chr4.3:54154774-54154793 MS.gene45340:intron 20.0%
! AAAATTTGCAACCCATTTAT+AGG - chr4.3:54154238-54154257 MS.gene45340:intron 25.0%
! AAATGCTTATAGAATGTACT+TGG - chr4.3:54155288-54155307 MS.gene45340:CDS 25.0%
! AAATTGCAAAAAAATTGGAC+CGG - chr4.3:54154359-54154378 MS.gene45340:intron 25.0%
! AAATTTGCAACCCATTTATA+GGG - chr4.3:54154239-54154258 MS.gene45340:intron 25.0%
! AATACAAGCATTTCATTAGT+TGG + chr4.3:54155010-54155029 None:intergenic 25.0%
! AATCGAATTTCAGTTATTTG+TGG - chr4.3:54154632-54154651 MS.gene45340:intron 25.0%
! ACAATATGGGTATGTAATAT+GGG - chr4.3:54155055-54155074 MS.gene45340:intron 25.0%
! ATACAAGCATTTCATTAGTT+GGG + chr4.3:54155009-54155028 None:intergenic 25.0%
! ATTTGTGGAAACATATCAAT+TGG - chr4.3:54154647-54154666 MS.gene45340:intron 25.0%
! CCAGAATTATTATAAAACCT+GGG - chr4.3:54154210-54154229 MS.gene45340:intron 25.0%
! TTATGTAAACCCCTATAAAT+GGG + chr4.3:54154252-54154271 None:intergenic 25.0%
!! AAAATTGTCTAAGTGTGTTT+TGG - chr4.3:54153976-54153995 MS.gene45340:intron 25.0%
!! GATTAATTTGGAAAAACAGT+AGG - chr4.3:54154745-54154764 MS.gene45340:intron 25.0%
!!! CAATTTTTCAAACAGCTTAT+AGG + chr4.3:54153963-54153982 None:intergenic 25.0%
!!! TATTGAATCCTTTAACTTGT+TGG - chr4.3:54155026-54155045 MS.gene45340:intron 25.0%
!!! TTTGAGTTCATTTTCAAAGA+AGG + chr4.3:54153797-54153816 None:intergenic 25.0%
AATTTGCAACCCATTTATAG+GGG - chr4.3:54154240-54154259 MS.gene45340:intron 30.0%
ATTTACTATAGAAGTTCCCA+GGG + chr4.3:54154820-54154839 None:intergenic 30.0%
ATTTCTCTAATGAATTCCCT+GGG - chr4.3:54154801-54154820 MS.gene45340:intron 30.0%
CAATGTCGATATTGTTAGAT+TGG + chr4.3:54154918-54154937 None:intergenic 30.0%
CACAATATGGGTATGTAATA+TGG - chr4.3:54155054-54155073 MS.gene45340:intron 30.0%
CTCTGAATTATATATATCCG+CGG - chr4.3:54154722-54154741 MS.gene45340:intron 30.0%
CTTATGTAAACCCCTATAAA+TGG + chr4.3:54154253-54154272 None:intergenic 30.0%
GCCAGAATTATTATAAAACC+TGG - chr4.3:54154209-54154228 MS.gene45340:intron 30.0%
TAATACATCAACTTAGTCGT+TGG - chr4.3:54154467-54154486 MS.gene45340:intron 30.0%
TCTGAATTATATATATCCGC+GGG - chr4.3:54154723-54154742 MS.gene45340:intron 30.0%
! ATAGAAGCATACAATGAAGA+AGG + chr4.3:54153889-54153908 None:intergenic 30.0%
! CTGTGTTTGTGAATTTTATG+TGG + chr4.3:54154852-54154871 None:intergenic 30.0%
! GTATGTAATATGGGATGTTT+TGG - chr4.3:54155064-54155083 MS.gene45340:intron 30.0%
! TTGTTGGTTACTCACAATAT+GGG - chr4.3:54155042-54155061 MS.gene45340:intron 30.0%
!! ACGACTAAGTTGATGTATTA+AGG + chr4.3:54154467-54154486 None:intergenic 30.0%
!! ACTAAGTTGATGTATTAAGG+GGG + chr4.3:54154464-54154483 None:intergenic 30.0%
!! CCCAGGTTTTATAATAATTC+TGG + chr4.3:54154213-54154232 None:intergenic 30.0%
!! CGACTAAGTTGATGTATTAA+GGG + chr4.3:54154466-54154485 None:intergenic 30.0%
!! GACTAAGTTGATGTATTAAG+GGG + chr4.3:54154465-54154484 None:intergenic 30.0%
AATGAACTTGACAGAAACAG+TGG - chr4.3:54153706-54153725 MS.gene45340:CDS 35.0%
AGCATAAAAAAGAGTGGAGT+TGG + chr4.3:54153929-54153948 None:intergenic 35.0%
AGTGGAAGAGATAGTGAAAT+TGG - chr4.3:54153735-54153754 MS.gene45340:CDS 35.0%
ATATATCCGCGGGATTAATT+TGG - chr4.3:54154733-54154752 MS.gene45340:intron 35.0%
CAATATCGACATTGTCAGTT+AGG - chr4.3:54154924-54154943 MS.gene45340:intron 35.0%
CAGGTTATGTCATGTATTCT+TGG - chr4.3:54153863-54153882 MS.gene45340:CDS 35.0%
CATTTACTATAGAAGTTCCC+AGG + chr4.3:54154821-54154840 None:intergenic 35.0%
CATTTCTCTAATGAATTCCC+TGG - chr4.3:54154800-54154819 MS.gene45340:intron 35.0%
CGCGGATATATATAATTCAG+AGG + chr4.3:54154724-54154743 None:intergenic 35.0%
CTGGGAACTTCTATAGTAAA+TGG - chr4.3:54154819-54154838 MS.gene45340:intron 35.0%
GAACTCAAAAAGAAGAACAG+TGG - chr4.3:54153808-54153827 MS.gene45340:CDS 35.0%
GTGAGTAACCAACAAGTTAA+AGG + chr4.3:54155037-54155056 None:intergenic 35.0%
GTTGACAGCATAAAAAAGAG+TGG + chr4.3:54153935-54153954 None:intergenic 35.0%
TGAAACAGTTGAAAGGTCTT+GGG - chr4.3:54154873-54154892 MS.gene45340:intron 35.0%
TGATTTGAGCAAAAGGCTAA+AGG + chr4.3:54154431-54154450 None:intergenic 35.0%
! CTTGTTGGTTACTCACAATA+TGG - chr4.3:54155041-54155060 MS.gene45340:intron 35.0%
! GTTTTTCCAAATTAATCCCG+CGG + chr4.3:54154742-54154761 None:intergenic 35.0%
! TTTTGTAAGTGTGACTCAGT+TGG - chr4.3:54154122-54154141 MS.gene45340:intron 35.0%
!! ACTCAACTGATTTGAGCAAA+AGG + chr4.3:54154438-54154457 None:intergenic 35.0%
!!! GTTGCAAATTTTTTTTGCCC+AGG + chr4.3:54154230-54154249 None:intergenic 35.0%
!!! TTTTGGTTCTTCTGTAGTGA+AGG - chr4.3:54155081-54155100 MS.gene45340:intron 35.0%
AAGCTTGGATATTGGTATGC+AGG - chr4.3:54154146-54154165 MS.gene45340:intron 40.0%
ACACAGCTGAAACAGTTGAA+AGG - chr4.3:54154866-54154885 MS.gene45340:intron 40.0%
ACATGACATAACCTGCAAGT+TGG + chr4.3:54153858-54153877 None:intergenic 40.0%
AGCTTGGATATTGGTATGCA+GGG - chr4.3:54154147-54154166 MS.gene45340:intron 40.0%
CAGAAACAGTGGTAAAGTAG+TGG - chr4.3:54153717-54153736 MS.gene45340:CDS 40.0%
CATGACATAACCTGCAAGTT+GGG + chr4.3:54153857-54153876 None:intergenic 40.0%
CTGAAACAGTTGAAAGGTCT+TGG - chr4.3:54154872-54154891 MS.gene45340:intron 40.0%
CTGAATCTGTACAAGAAGCA+TGG - chr4.3:54155220-54155239 MS.gene45340:CDS 40.0%
GCAGAACAAGAAAAGTTTCG+CGG - chr4.3:54155161-54155180 MS.gene45340:CDS 40.0%
GCATTTCATTAGTTGGGAGT+TGG + chr4.3:54155003-54155022 None:intergenic 40.0%
! CATGTTGACTGTTTGGTTGA+AGG - chr4.3:54155247-54155266 MS.gene45340:CDS 40.0%
! TGGCTTTCATGTTGACTGTT+TGG - chr4.3:54155240-54155259 MS.gene45340:CDS 40.0%
!! CAGTTGGTAAGCTTGGATAT+TGG - chr4.3:54154138-54154157 MS.gene45340:intron 40.0%
CTTCTGTAGTGAAGGAGCAA+TGG - chr4.3:54155089-54155108 MS.gene45340:intron 45.0%
GAAAGGTCTTGGGTTCATAC+TGG - chr4.3:54154883-54154902 MS.gene45340:intron 45.0%
GAAGCGAGTGATTCGTGTTT+GGG + chr4.3:54153775-54153794 None:intergenic 45.0%
TATGCAGGGTTCGAGATTCT+GGG - chr4.3:54154161-54154180 MS.gene45340:intron 45.0%
TCTTGTACAGATTCAGCGCA+GGG + chr4.3:54155216-54155235 None:intergenic 45.0%
TGAGCAAAAGGCTAAAGGCA+GGG + chr4.3:54154426-54154445 None:intergenic 45.0%
TTCTTGTACAGATTCAGCGC+AGG + chr4.3:54155217-54155236 None:intergenic 45.0%
TTGAGCAAAAGGCTAAAGGC+AGG + chr4.3:54154427-54154446 None:intergenic 45.0%
!! TGTGACTCAGTTGGTAAGCT+TGG - chr4.3:54154131-54154150 MS.gene45340:intron 45.0%
CGGAGAGGTTCTACTGAAAG+CGG - chr4.3:54155126-54155145 MS.gene45340:CDS 50.0%
CTGTAGTGAAGGAGCAATGG+AGG - chr4.3:54155092-54155111 MS.gene45340:intron 50.0%
CTTGTACAGATTCAGCGCAG+GGG + chr4.3:54155215-54155234 None:intergenic 50.0%
GAGCAAAAGGCTAAAGGCAG+GGG + chr4.3:54154425-54154444 None:intergenic 50.0%
GGAAGCGAGTGATTCGTGTT+TGG + chr4.3:54153776-54153795 None:intergenic 50.0%
GGTTCGAACCATTTAGCCAG+TGG - chr4.3:54154182-54154201 MS.gene45340:intron 50.0%
GTATGCAGGGTTCGAGATTC+TGG - chr4.3:54154160-54154179 MS.gene45340:intron 50.0%
TAGTGAAGGAGCAATGGAGG+AGG - chr4.3:54155095-54155114 MS.gene45340:CDS 50.0%
TCGAACCATTTAGCCAGTGG+CGG - chr4.3:54154185-54154204 MS.gene45340:intron 50.0%
TGCTTCACGTTGATCCGTTG+AGG - chr4.3:54155188-54155207 MS.gene45340:CDS 50.0%
!! AATAATTCTGGCTCCGCCAC+TGG + chr4.3:54154201-54154220 None:intergenic 50.0%
AAGGAGCAATGGAGGAGGCA+AGG - chr4.3:54155100-54155119 MS.gene45340:CDS 55.0%
CAAAAGGCTAAAGGCAGGGG+CGG + chr4.3:54154422-54154441 None:intergenic 55.0%
CAATGGAGGAGGCAAGGACA+CGG - chr4.3:54155106-54155125 MS.gene45340:CDS 55.0%
CACGTTGATGCCCAACTTGC+AGG - chr4.3:54153844-54153863 MS.gene45340:CDS 55.0%
GCTTCTATCACCAAGCTCGC+AGG - chr4.3:54153901-54153920 MS.gene45340:intron 55.0%
TAAAGGCAGGGGCGGTTCTA+AGG + chr4.3:54154414-54154433 None:intergenic 55.0%
GGAGTTGGAACCTGCGAGCT+TGG + chr4.3:54153914-54153933 None:intergenic 60.0%
TTCAGCGCAGGGGTCCTCAA+CGG + chr4.3:54155205-54155224 None:intergenic 60.0%
! TGGCTCCGCCACTGGCTAAA+TGG + chr4.3:54154193-54154212 None:intergenic 60.0%
!! TTCTAAGGCCCAGCGAGCCT+GGG + chr4.3:54154399-54154418 None:intergenic 60.0%
AGACAACTGCCCAGGCTCGC+TGG - chr4.3:54154387-54154406 MS.gene45340:intron 65.0%
AGCGAGCCTGGGCAGTTGTC+TGG + chr4.3:54154388-54154407 None:intergenic 65.0%
CTGGGCAGTTGTCTGGGCTC+CGG + chr4.3:54154381-54154400 None:intergenic 65.0%
GACAACTGCCCAGGCTCGCT+GGG - chr4.3:54154388-54154407 MS.gene45340:intron 65.0%
GAGGAGGCAAGGACACGGAG+AGG - chr4.3:54155111-54155130 MS.gene45340:CDS 65.0%
GCGAGCCTGGGCAGTTGTCT+GGG + chr4.3:54154387-54154406 None:intergenic 65.0%
!! GTTCTAAGGCCCAGCGAGCC+TGG + chr4.3:54154400-54154419 None:intergenic 65.0%
CGGAGCCCAGACAACTGCCC+AGG - chr4.3:54154379-54154398 MS.gene45340:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 54153688 54155327 54153688 ID=MS.gene45340
chr4.3 mRNA 54153688 54155327 54153688 ID=MS.gene45340.t1;Parent=MS.gene45340
chr4.3 exon 54155093 54155327 54155093 ID=MS.gene45340.t1.exon1;Parent=MS.gene45340.t1
chr4.3 CDS 54155093 54155327 54155093 ID=cds.MS.gene45340.t1;Parent=MS.gene45340.t1
chr4.3 exon 54153688 54153917 54153688 ID=MS.gene45340.t1.exon2;Parent=MS.gene45340.t1
chr4.3 CDS 54153688 54153917 54153688 ID=cds.MS.gene45340.t1;Parent=MS.gene45340.t1
Gene Sequence

>MS.gene45340

ATGGCAAATGTGGAGATTAATGAACTTGACAGAAACAGTGGTAAAGTAGTGGAAGAGATAGTGAAATTGGAGAGAAAGATATTTCCCAAACACGAATCACTCGCTTCCTTCTTTGAAAATGAACTCAAAAAGAAGAACAGTGGATTGCTTTATCTTCACGTTGATGCCCAACTTGCAGGTTATGTCATGTATTCTTGGCCTTCTTCATTGTATGCTTCTATCACCAAGCTCGCAGGTTCCAACTCCACTCTTTTTTATGCTGTCAACTTATGCCTATAAGCTGTTTGAAAAATTGTCTAAGTGTGTTTTGGTGTTTGAAAATTTAGTGACTCAAATTTATAAAATATCAATATATACTCTCAAATTAAAAAAAAAAAAAAGTCATTTCATTTGTTTTCTTTTCTAAAATATCTTTTAAGAAAATATTCATGAACTTTTGTAAGTGTGACTCAGTTGGTAAGCTTGGATATTGGTATGCAGGGTTCGAGATTCTGGGTTCGAACCATTTAGCCAGTGGCGGAGCCAGAATTATTATAAAACCTGGGCAAAAAAAATTTGCAACCCATTTATAGGGGTTTACATAAGAAATTGCAAGCAAATAATCAAAAAATCTAAAGAAATTATCCAATTTATAGGGGTTAATATAAGAAATTCAGAGAGATATACATAAGAAATTGCAAAAAAATTGGACCGGAGCCCAGACAACTGCCCAGGCTCGCTGGGCCTTAGAACCGCCCCTGCCTTTAGCCTTTTGCTCAAATCAGTTGAGTTATCCCCCTTAATACATCAACTTAGTCGTTGGATAATTACTCCTATTATATTTCAATCGTTTAGAGACTGAATTAGACAAAAAGTGAAAGTTAAAGTTTTAAAACATACTAAATTGATTAACTATCTTTTTAGTCTGAAGATGAAGTGACAAAAATTGAAGATACAGATCAAGAAATCGAATTTCAGTTATTTGTGGAAACATATCAATTGGTAGAACTCTCGATAAAAGAAAAAGATGTTGTATATGAATCACTTACATATTCCTCTGAATTATATATATCCGCGGGATTAATTTGGAAAAACAGTAGGGATATCCAAGAACAGATTATTTTTATTGGAAACATTTCTCTAATGAATTCCCTGGGAACTTCTATAGTAAATGGAAAGTCACCACATAAAATTCACAAACACAGCTGAAACAGTTGAAAGGTCTTGGGTTCATACTGGATGAAATGTCCAATCTAACAATATCGACATTGTCAGTTAGGCTACAATTAACTATCTTTAATATAAGGGAATAAATAATAATTCTGCAAATTGTCCAACTCCCAACTAATGAAATGCTTGTATTGAATCCTTTAACTTGTTGGTTACTCACAATATGGGTATGTAATATGGGATGTTTTGGTTCTTCTGTAGTGAAGGAGCAATGGAGGAGGCAAGGACACGGAGAGGTTCTACTGAAAGCGGCAATCGAGAAATGCAGAACAAGAAAAGTTTCGCGGATAATGCTTCACGTTGATCCGTTGAGGACCCCTGCGCTGAATCTGTACAAGAAGCATGGCTTTCATGTTGACTGTTTGGTTGAAGGCTATTACTCTTCTGATAGAAATGCTTATAGAATGTACTTGGACTTCGATTCAAATTGA

Protein sequence

>MS.gene45340.t1

MANVEINELDRNSGKVVEEIVKLERKIFPKHESLASFFENELKKKNSGLLYLHVDAQLAGYVMYSWPSSLYASITKLAVKEQWRRQGHGEVLLKAAIEKCRTRKVSRIMLHVDPLRTPALNLYKKHGFHVDCLVEGYYSSDRNAYRMYLDFDSN