AlfalfaGEDB | MS.gene47819

M. sativa cultivar XinJiangDaYe / MS.gene47819

KEGG: K02922
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01907.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47819.t1	XP_003597753.2	100	94	0	0	1	94	1	94	4.10E-45	190.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47819.t1	Q8LEM8	88.3	94	11	0	1	94	1	94	6.3e-42	171.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47819.t1	B7FGF1	100.0	94	0	0	1	94	1	94	3.0e-45	190.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene47819	0.817434	3.30E-52	-1.69E-46
MS.gene051330	MS.gene47819	0.838032	3.66E-57	-1.69E-46
MS.gene051746	MS.gene47819	0.805991	1.02E-49	-1.69E-46
MS.gene051748	MS.gene47819	0.810845	9.41E-51	-1.69E-46
MS.gene051903	MS.gene47819	0.855418	6.39E-62	-1.69E-46
MS.gene052947	MS.gene47819	0.832738	7.96E-56	-1.69E-46
MS.gene053952	MS.gene47819	0.81811	2.33E-52	-1.69E-46
MS.gene054937	MS.gene47819	0.823909	1.07E-53	-1.69E-46
MS.gene056601	MS.gene47819	0.803318	3.69E-49	-1.69E-46
MS.gene056631	MS.gene47819	0.841716	4.01E-58	-1.69E-46
MS.gene057705	MS.gene47819	0.816125	6.50E-52	-1.69E-46
MS.gene058354	MS.gene47819	0.805705	1.17E-49	-1.69E-46
MS.gene06001	MS.gene47819	0.860551	1.91E-63	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene47819	MS.gene33384	PPI
MS.gene47819	MS.gene85108	PPI
MS.gene47819	MS.gene24930	PPI
MS.gene47819	MS.gene052947	PPI
MS.gene47819	MS.gene068255	PPI
MS.gene47819	MS.gene36310	PPI
MS.gene47819	MS.gene85110	PPI
MS.gene052947	MS.gene47819	PPI
MS.gene33384	MS.gene47819	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47819.t1	MTR_2g101900	100.000	94	0	0	1	94	1	94	4.84e-64	188
MS.gene47819.t1	MTR_0147s0070	96.809	94	3	0	1	94	1	94	1.38e-62	184

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene47819.t1	AT3G16080	88.298	94	11	1	94	1	94	7.27e-57	170
MS.gene47819.t1	AT1G52300	88.298	94	11	1	94	1	94	1.73e-56	169
MS.gene47819.t1	AT1G15250	87.234	94	12	1	94	1	94	9.08e-56	167
MS.gene47819.t1	AT1G15250	87.234	94	12	1	94	1	94	9.08e-56	167
MS.gene47819.t1	AT3G16080	93.333	45	3	1	45	1	45	9.58e-25	89.0

Find 26 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTAAAACCGCTCTTGAAT+CGG	0.373242	2.1:-3348247	None:intergenic
AAGCAGAGCATCTACTCTTC+TGG	0.402579	2.1:-3348025	None:intergenic
CTTACCCAGCTGCTCGTGTT+AGG	0.412584	2.1:+3348051	MS.gene47819:CDS
CATTTCCTAACACGAGCAGC+TGG	0.431351	2.1:-3348056	None:intergenic
GAATTGCAGGGTAAGGGAAC+TGG	0.450011	2.1:+3347933	MS.gene47819:intron
CGTAGAAAGACTACTGGAAC+TGG	0.453204	2.1:+3348194	MS.gene47819:CDS
TTTAGATAACTGGAGTGAAA+AGG	0.467360	2.1:+3348166	MS.gene47819:intron
CAGTTCCAGTAGTCTTTCTA+CGG	0.470228	2.1:-3348193	None:intergenic
GGTAAGGGAACTGGAAGTTT+CGG	0.474527	2.1:+3347942	MS.gene47819:CDS
AGACTCACACACTCTGTGTC+AGG	0.478990	2.1:+3347979	MS.gene47819:CDS
TGAAGCTGCACCTAGGAAGA+AGG	0.512832	2.1:+3348375	MS.gene47819:CDS
AGCATCTACTCTTCTGGAGA+TGG	0.532321	2.1:-3348019	None:intergenic
GCAATCCGTAGAAAGACTAC+TGG	0.532707	2.1:+3348188	MS.gene47819:CDS
GTGAATGAATTGCAGGGTAA+GGG	0.538112	2.1:+3347927	MS.gene47819:intron
GACGCAGCTCCCTTCTTCCT+AGG	0.557312	2.1:-3348385	None:intergenic
GAAGCTGCACCTAGGAAGAA+GGG	0.598975	2.1:+3348376	MS.gene47819:CDS
CACACACTCTGTGTCAGGTG+TGG	0.600659	2.1:+3347984	MS.gene47819:CDS
CTACTGGAACTGGTAGGATG+AGG	0.613944	2.1:+3348204	MS.gene47819:CDS
CTGGAAGTTTCGGTAAGAGA+AGG	0.618981	2.1:+3347952	MS.gene47819:CDS
CAGGAACTGAAGCTGCACCT+AGG	0.634185	2.1:+3348368	MS.gene47819:intron
ATTTCCTAACACGAGCAGCT+GGG	0.638199	2.1:-3348055	None:intergenic
GAAAGACTACTGGAACTGGT+AGG	0.653500	2.1:+3348198	MS.gene47819:CDS
TCTGGAGATGGAAACTGCGG+CGG	0.656436	2.1:-3348007	None:intergenic
TCTTCTGGAGATGGAAACTG+CGG	0.675301	2.1:-3348010	None:intergenic
GTTCCTCGCCGATTCAAGAG+CGG	0.676604	2.1:+3348239	MS.gene47819:CDS
AAACCGCTCTTGAATCGGCG+AGG	0.683649	2.1:-3348242	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTTAATTTGATTTGTAATTA+GGG	+	chr2.1:3348098-3348117	MS.gene47819:intron	15.0%
!!!	TGTTAATTTGATTTGTAATT+AGG	+	chr2.1:3348097-3348116	MS.gene47819:intron	15.0%
!!!	GATTATTGATTTTAGATAAC+TGG	+	chr2.1:3348156-3348175	MS.gene47819:intron	20.0%
!!	TATAGATTGATGATAGATGA+AGG	-	chr2.1:3347865-3347884	None:intergenic	25.0%
!!!	GTTGTGGTTTTTTTTAATAC+AGG	+	chr2.1:3348349-3348368	MS.gene47819:intron	25.0%
	CCACAACATATCAATGATTA+AGG	-	chr2.1:3348336-3348355	None:intergenic	30.0%
	CCTTAATCATTGATATGTTG+TGG	+	chr2.1:3348333-3348352	MS.gene47819:intron	30.0%
	GTTTATGTGAATGAATTGCA+GGG	+	chr2.1:3347921-3347940	MS.gene47819:intron	30.0%
	TTTAGATAACTGGAGTGAAA+AGG	+	chr2.1:3348166-3348185	MS.gene47819:intron	30.0%
	CGTTTATGTGAATGAATTGC+AGG	+	chr2.1:3347920-3347939	MS.gene47819:intron	35.0%
	CTATACGATTTCGTATATGC+TGG	+	chr2.1:3347880-3347899	MS.gene47819:intron	35.0%
!	AGATTGATGATAGATGAAGG+AGG	-	chr2.1:3347862-3347881	None:intergenic	35.0%
	CAGTTCCAGTAGTCTTTCTA+CGG	-	chr2.1:3348196-3348215	None:intergenic	40.0%
	GTGAATGAATTGCAGGGTAA+GGG	+	chr2.1:3347927-3347946	MS.gene47819:intron	40.0%
	TGATGATAGATGAAGGAGGA+AGG	-	chr2.1:3347858-3347877	None:intergenic	40.0%
	TGTGAATGAATTGCAGGGTA+AGG	+	chr2.1:3347926-3347945	MS.gene47819:intron	40.0%
!!	CTCTAAAACCGCTCTTGAAT+CGG	-	chr2.1:3348250-3348269	None:intergenic	40.0%
!!!	ATTCAAGAGCGGTTTTAGAG+AGG	+	chr2.1:3348250-3348269	MS.gene47819:CDS	40.0%
!!!	TTCAAGAGCGGTTTTAGAGA+GGG	+	chr2.1:3348251-3348270	MS.gene47819:CDS	40.0%
	ATTTCCTAACACGAGCAGCT+GGG	-	chr2.1:3348058-3348077	None:intergenic	45.0%
	CGTAGAAAGACTACTGGAAC+TGG	+	chr2.1:3348194-3348213	MS.gene47819:CDS	45.0%
	CTGGAAGTTTCGGTAAGAGA+AGG	+	chr2.1:3347952-3347971	MS.gene47819:CDS	45.0%
	GAAAGACTACTGGAACTGGT+AGG	+	chr2.1:3348198-3348217	MS.gene47819:CDS	45.0%
	GCAATCCGTAGAAAGACTAC+TGG	+	chr2.1:3348188-3348207	MS.gene47819:CDS	45.0%
	GGTAAGGGAACTGGAAGTTT+CGG	+	chr2.1:3347942-3347961	MS.gene47819:CDS	45.0%
!	AAGCAGAGCATCTACTCTTC+TGG	-	chr2.1:3348028-3348047	None:intergenic	45.0%
!	AGCATCTACTCTTCTGGAGA+TGG	-	chr2.1:3348022-3348041	None:intergenic	45.0%
!	TCTTCTGGAGATGGAAACTG+CGG	-	chr2.1:3348013-3348032	None:intergenic	45.0%
	AGACTCACACACTCTGTGTC+AGG	+	chr2.1:3347979-3347998	MS.gene47819:CDS	50.0%
	CATTTCCTAACACGAGCAGC+TGG	-	chr2.1:3348059-3348078	None:intergenic	50.0%
	CTACTGGAACTGGTAGGATG+AGG	+	chr2.1:3348204-3348223	MS.gene47819:CDS	50.0%
	GAAGCTGCACCTAGGAAGAA+GGG	+	chr2.1:3348376-3348395	MS.gene47819:CDS	50.0%
	GAATTGCAGGGTAAGGGAAC+TGG	+	chr2.1:3347933-3347952	MS.gene47819:intron	50.0%
	TGAAGCTGCACCTAGGAAGA+AGG	+	chr2.1:3348375-3348394	MS.gene47819:CDS	50.0%
	AAACCGCTCTTGAATCGGCG+AGG	-	chr2.1:3348245-3348264	None:intergenic	55.0%
	CACACACTCTGTGTCAGGTG+TGG	+	chr2.1:3347984-3348003	MS.gene47819:CDS	55.0%
	CAGGAACTGAAGCTGCACCT+AGG	+	chr2.1:3348368-3348387	MS.gene47819:intron	55.0%
	CTTACCCAGCTGCTCGTGTT+AGG	+	chr2.1:3348051-3348070	MS.gene47819:CDS	55.0%
	TCTGGAGATGGAAACTGCGG+CGG	-	chr2.1:3348010-3348029	None:intergenic	55.0%
!!	GTTCCTCGCCGATTCAAGAG+CGG	+	chr2.1:3348239-3348258	MS.gene47819:CDS	55.0%
	GACGCAGCTCCCTTCTTCCT+AGG	-	chr2.1:3348388-3348407	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	3347843	3348414	3347843	ID=MS.gene47819
chr2.1	mRNA	3347843	3348414	3347843	ID=MS.gene47819.t1;Parent=MS.gene47819
chr2.1	exon	3347843	3347845	3347843	ID=MS.gene47819.t1.exon1;Parent=MS.gene47819.t1
chr2.1	CDS	3347843	3347845	3347843	ID=cds.MS.gene47819.t1;Parent=MS.gene47819.t1
chr2.1	exon	3347942	3348077	3347942	ID=MS.gene47819.t1.exon2;Parent=MS.gene47819.t1
chr2.1	CDS	3347942	3348077	3347942	ID=cds.MS.gene47819.t1;Parent=MS.gene47819.t1
chr2.1	exon	3348171	3348272	3348171	ID=MS.gene47819.t1.exon3;Parent=MS.gene47819.t1
chr2.1	CDS	3348171	3348272	3348171	ID=cds.MS.gene47819.t1;Parent=MS.gene47819.t1
chr2.1	exon	3348371	3348414	3348371	ID=MS.gene47819.t1.exon4;Parent=MS.gene47819.t1
chr2.1	CDS	3348371	3348414	3348371	ID=cds.MS.gene47819.t1;Parent=MS.gene47819.t1

Gene Sequence

>MS.gene47819

ATGGTAACATCTCCTTCCTCCTTCATCTATCATCAATCTATACGATTTCGTATATGCTGGTTATGTTTAGATTAATACGTTTATGTGAATGAATTGCAGGGTAAGGGAACTGGAAGTTTCGGTAAGAGAAGGAACAAGACTCACACACTCTGTGTCAGGTGTGGCCGCCGCAGTTTCCATCTCCAGAAGAGTAGATGCTCTGCTTGTGCTTACCCAGCTGCTCGTGTTAGGAAATGTAATTAGTCTTAATCTATTGTTAATTTGATTTGTAATTAGGGTTTATGTATGTTTGCTTTATTAATTAATTCATTTTGATTATTGATTTTAGATAACTGGAGTGAAAAGGCAATCCGTAGAAAGACTACTGGAACTGGTAGGATGAGGTACTTGCGCAACGTTCCTCGCCGATTCAAGAGCGGTTTTAGAGAGGGTAATTAATCTTTTTCTAGCTTAATATGTTCGATTTAGTTTCTTAAGATTCAGTTTAGATCCTTAATCATTGATATGTTGTGGTTTTTTTTAATACAGGAACTGAAGCTGCACCTAGGAAGAAGGGAGCTGCGTCTGCTTAA

Protein sequence

>MS.gene47819.t1

MGKGTGSFGKRRNKTHTLCVRCGRRSFHLQKSRCSACAYPAARVRKYNWSEKAIRRKTTGTGRMRYLRNVPRRFKSGFREGTEAAPRKKGAASA