AlfalfaGEDB | MS.gene85108

M. sativa cultivar XinJiangDaYe / MS.gene85108

KEGG: K02922
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF01907.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85108.t1	XP_003597753.2	100	94	0	0	1	94	1	94	4.10E-45	190.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85108.t1	Q8LEM8	88.3	94	11	0	1	94	1	94	6.3e-42	171.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85108.t1	B7FGF1	100.0	94	0	0	1	94	1	94	3.0e-45	190.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050277	MS.gene85108	0.838112	3.49E-57	-1.69E-46
MS.gene050290	MS.gene85108	0.84437	7.89E-59	-1.69E-46
MS.gene051063	MS.gene85108	0.813081	3.07E-51	-1.69E-46
MS.gene051330	MS.gene85108	0.858169	9.91E-63	-1.69E-46
MS.gene051746	MS.gene85108	0.832432	9.49E-56	-1.69E-46
MS.gene051748	MS.gene85108	0.830789	2.41E-55	-1.69E-46
MS.gene051903	MS.gene85108	0.865185	7.09E-65	-1.69E-46
MS.gene052947	MS.gene85108	0.843182	1.64E-58	-1.69E-46
MS.gene053952	MS.gene85108	0.849453	3.20E-60	-1.69E-46
MS.gene054937	MS.gene85108	0.859237	4.75E-63	-1.69E-46
MS.gene055162	MS.gene85108	0.804576	2.02E-49	-1.69E-46
MS.gene055780	MS.gene85108	0.807907	4.02E-50	-1.69E-46
MS.gene056075	MS.gene85108	0.820649	6.13E-53	-1.69E-46
MS.gene056601	MS.gene85108	0.838061	3.60E-57	-1.69E-46
MS.gene056631	MS.gene85108	0.848497	5.90E-60	-1.69E-46
MS.gene057094	MS.gene85108	0.831939	1.26E-55	-1.69E-46
MS.gene057705	MS.gene85108	0.807815	4.20E-50	-1.69E-46
MS.gene058354	MS.gene85108	0.842145	3.09E-58	-1.69E-46
MS.gene059726	MS.gene85108	0.804955	1.68E-49	-1.69E-46
MS.gene06001	MS.gene85108	0.881421	2.43E-70	-1.69E-46
MS.gene061145	MS.gene85108	0.823984	1.03E-53	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene85108	MS.gene24930	PPI
MS.gene85108	MS.gene36310	PPI
MS.gene85108	MS.gene85110	PPI
MS.gene052947	MS.gene85108	PPI
MS.gene33384	MS.gene85108	PPI
MS.gene85108	MS.gene068255	PPI
MS.gene47819	MS.gene85108	PPI
MS.gene24930	MS.gene85108	PPI
MS.gene36310	MS.gene85108	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85108.t1	MTR_2g101900	100.000	94	0	0	1	94	1	94	4.84e-64	188
MS.gene85108.t1	MTR_0147s0070	96.809	94	3	0	1	94	1	94	1.38e-62	184

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85108.t1	AT3G16080	88.298	94	11	1	94	1	94	7.27e-57	170
MS.gene85108.t1	AT1G52300	88.298	94	11	1	94	1	94	1.73e-56	169
MS.gene85108.t1	AT1G15250	87.234	94	12	1	94	1	94	9.08e-56	167
MS.gene85108.t1	AT1G15250	87.234	94	12	1	94	1	94	9.08e-56	167
MS.gene85108.t1	AT3G16080	93.333	45	3	1	45	1	45	9.58e-25	89.0

Find 26 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTAAAACCGCTCTTGAAT+CGG	0.373242	2.3:-2250173	None:intergenic
AAGCAGAGCATCTACTCTTC+TGG	0.402579	2.3:-2249951	None:intergenic
CTTACCCAGCTGCTCGTGTT+AGG	0.412584	2.3:+2249977	MS.gene85108:CDS
CATTTCCTAACACGAGCAGC+TGG	0.431351	2.3:-2249982	None:intergenic
GAATTGCAGGGTAAGGGAAC+TGG	0.450011	2.3:+2249859	MS.gene85108:intron
CGTAGAAAGACTACTGGAAC+TGG	0.453204	2.3:+2250120	MS.gene85108:CDS
TTTAGATAACTGGAGTGAAA+AGG	0.467360	2.3:+2250092	MS.gene85108:intron
CAGTTCCAGTAGTCTTTCTA+CGG	0.470228	2.3:-2250119	None:intergenic
GGTAAGGGAACTGGAAGTTT+CGG	0.474527	2.3:+2249868	MS.gene85108:CDS
AGACTCACACACTCTGTGTC+AGG	0.478990	2.3:+2249905	MS.gene85108:CDS
TGAAGCTGCACCTAGGAAGA+AGG	0.512832	2.3:+2250301	MS.gene85108:CDS
AGCATCTACTCTTCTGGAGA+TGG	0.532321	2.3:-2249945	None:intergenic
GCAATCCGTAGAAAGACTAC+TGG	0.532707	2.3:+2250114	MS.gene85108:CDS
GTGAATGAATTGCAGGGTAA+GGG	0.538112	2.3:+2249853	MS.gene85108:intron
GACGCAGCTCCCTTCTTCCT+AGG	0.557312	2.3:-2250311	None:intergenic
GAAGCTGCACCTAGGAAGAA+GGG	0.598975	2.3:+2250302	MS.gene85108:CDS
CACACACTCTGTGTCAGGTG+TGG	0.600659	2.3:+2249910	MS.gene85108:CDS
CTACTGGAACTGGTAGGATG+AGG	0.613944	2.3:+2250130	MS.gene85108:CDS
CTGGAAGTTTCGGTAAGAGA+AGG	0.618981	2.3:+2249878	MS.gene85108:CDS
CAGGAACTGAAGCTGCACCT+AGG	0.634185	2.3:+2250294	MS.gene85108:intron
ATTTCCTAACACGAGCAGCT+GGG	0.638199	2.3:-2249981	None:intergenic
GAAAGACTACTGGAACTGGT+AGG	0.653500	2.3:+2250124	MS.gene85108:CDS
TCTGGAGATGGAAACTGCGG+CGG	0.656436	2.3:-2249933	None:intergenic
TCTTCTGGAGATGGAAACTG+CGG	0.675301	2.3:-2249936	None:intergenic
GTTCCTCGCCGATTCAAGAG+CGG	0.676604	2.3:+2250165	MS.gene85108:CDS
AAACCGCTCTTGAATCGGCG+AGG	0.683649	2.3:-2250168	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTTAATTTGATTTGTAATTA+GGG	+	chr2.3:2250024-2250043	MS.gene85108:intron	15.0%
!!!	TGTTAATTTGATTTGTAATT+AGG	+	chr2.3:2250023-2250042	MS.gene85108:intron	15.0%
!!!	GATTATTGATTTTAGATAAC+TGG	+	chr2.3:2250082-2250101	MS.gene85108:intron	20.0%
!!	TATAGATTGATGATAGATGA+AGG	-	chr2.3:2249791-2249810	None:intergenic	25.0%
!!!	GTTGTGGTTTTTTTTAATAC+AGG	+	chr2.3:2250275-2250294	MS.gene85108:intron	25.0%
	CCACAACATATCAATGATTA+AGG	-	chr2.3:2250262-2250281	None:intergenic	30.0%
	CCTTAATCATTGATATGTTG+TGG	+	chr2.3:2250259-2250278	MS.gene85108:intron	30.0%
	GTTTATGTGAATGAATTGCA+GGG	+	chr2.3:2249847-2249866	MS.gene85108:intron	30.0%
	TTTAGATAACTGGAGTGAAA+AGG	+	chr2.3:2250092-2250111	MS.gene85108:intron	30.0%
	CGTTTATGTGAATGAATTGC+AGG	+	chr2.3:2249846-2249865	MS.gene85108:intron	35.0%
	CTATACGATTTCGTATATGC+TGG	+	chr2.3:2249806-2249825	MS.gene85108:intron	35.0%
!	AGATTGATGATAGATGAAGG+AGG	-	chr2.3:2249788-2249807	None:intergenic	35.0%
	CAGTTCCAGTAGTCTTTCTA+CGG	-	chr2.3:2250122-2250141	None:intergenic	40.0%
	GTGAATGAATTGCAGGGTAA+GGG	+	chr2.3:2249853-2249872	MS.gene85108:intron	40.0%
	TGATGATAGATGAAGGAGGA+AGG	-	chr2.3:2249784-2249803	None:intergenic	40.0%
	TGTGAATGAATTGCAGGGTA+AGG	+	chr2.3:2249852-2249871	MS.gene85108:intron	40.0%
!!	CTCTAAAACCGCTCTTGAAT+CGG	-	chr2.3:2250176-2250195	None:intergenic	40.0%
!!!	ATTCAAGAGCGGTTTTAGAG+AGG	+	chr2.3:2250176-2250195	MS.gene85108:CDS	40.0%
!!!	TTCAAGAGCGGTTTTAGAGA+GGG	+	chr2.3:2250177-2250196	MS.gene85108:CDS	40.0%
	ATTTCCTAACACGAGCAGCT+GGG	-	chr2.3:2249984-2250003	None:intergenic	45.0%
	CGTAGAAAGACTACTGGAAC+TGG	+	chr2.3:2250120-2250139	MS.gene85108:CDS	45.0%
	CTGGAAGTTTCGGTAAGAGA+AGG	+	chr2.3:2249878-2249897	MS.gene85108:CDS	45.0%
	GAAAGACTACTGGAACTGGT+AGG	+	chr2.3:2250124-2250143	MS.gene85108:CDS	45.0%
	GCAATCCGTAGAAAGACTAC+TGG	+	chr2.3:2250114-2250133	MS.gene85108:CDS	45.0%
	GGTAAGGGAACTGGAAGTTT+CGG	+	chr2.3:2249868-2249887	MS.gene85108:CDS	45.0%
!	AAGCAGAGCATCTACTCTTC+TGG	-	chr2.3:2249954-2249973	None:intergenic	45.0%
!	AGCATCTACTCTTCTGGAGA+TGG	-	chr2.3:2249948-2249967	None:intergenic	45.0%
!	TCTTCTGGAGATGGAAACTG+CGG	-	chr2.3:2249939-2249958	None:intergenic	45.0%
	AGACTCACACACTCTGTGTC+AGG	+	chr2.3:2249905-2249924	MS.gene85108:CDS	50.0%
	CATTTCCTAACACGAGCAGC+TGG	-	chr2.3:2249985-2250004	None:intergenic	50.0%
	CTACTGGAACTGGTAGGATG+AGG	+	chr2.3:2250130-2250149	MS.gene85108:CDS	50.0%
	GAAGCTGCACCTAGGAAGAA+GGG	+	chr2.3:2250302-2250321	MS.gene85108:CDS	50.0%
	GAATTGCAGGGTAAGGGAAC+TGG	+	chr2.3:2249859-2249878	MS.gene85108:intron	50.0%
	TGAAGCTGCACCTAGGAAGA+AGG	+	chr2.3:2250301-2250320	MS.gene85108:CDS	50.0%
	AAACCGCTCTTGAATCGGCG+AGG	-	chr2.3:2250171-2250190	None:intergenic	55.0%
	CACACACTCTGTGTCAGGTG+TGG	+	chr2.3:2249910-2249929	MS.gene85108:CDS	55.0%
	CAGGAACTGAAGCTGCACCT+AGG	+	chr2.3:2250294-2250313	MS.gene85108:intron	55.0%
	CTTACCCAGCTGCTCGTGTT+AGG	+	chr2.3:2249977-2249996	MS.gene85108:CDS	55.0%
	TCTGGAGATGGAAACTGCGG+CGG	-	chr2.3:2249936-2249955	None:intergenic	55.0%
!!	GTTCCTCGCCGATTCAAGAG+CGG	+	chr2.3:2250165-2250184	MS.gene85108:CDS	55.0%
	GACGCAGCTCCCTTCTTCCT+AGG	-	chr2.3:2250314-2250333	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	2249769	2250340	2249769	ID=MS.gene85108
chr2.3	mRNA	2249769	2250340	2249769	ID=MS.gene85108.t1;Parent=MS.gene85108
chr2.3	exon	2249769	2249771	2249769	ID=MS.gene85108.t1.exon1;Parent=MS.gene85108.t1
chr2.3	CDS	2249769	2249771	2249769	ID=cds.MS.gene85108.t1;Parent=MS.gene85108.t1
chr2.3	exon	2249868	2250003	2249868	ID=MS.gene85108.t1.exon2;Parent=MS.gene85108.t1
chr2.3	CDS	2249868	2250003	2249868	ID=cds.MS.gene85108.t1;Parent=MS.gene85108.t1
chr2.3	exon	2250097	2250198	2250097	ID=MS.gene85108.t1.exon3;Parent=MS.gene85108.t1
chr2.3	CDS	2250097	2250198	2250097	ID=cds.MS.gene85108.t1;Parent=MS.gene85108.t1
chr2.3	exon	2250297	2250340	2250297	ID=MS.gene85108.t1.exon4;Parent=MS.gene85108.t1
chr2.3	CDS	2250297	2250340	2250297	ID=cds.MS.gene85108.t1;Parent=MS.gene85108.t1

Gene Sequence

>MS.gene85108

ATGGTAACATCTCCTTCCTCCTTCATCTATCATCAATCTATACGATTTCGTATATGCTGGTTATGTTTAGATTAATACGTTTATGTGAATGAATTGCAGGGTAAGGGAACTGGAAGTTTCGGTAAGAGAAGGAACAAGACTCACACACTCTGTGTCAGGTGTGGCCGCCGCAGTTTCCATCTCCAGAAGAGTAGATGCTCTGCTTGTGCTTACCCAGCTGCTCGTGTTAGGAAATGTAATTAGTCTTAATCTATTGTTAATTTGATTTGTAATTAGGGTTTATGTATGTTTGCTTTATTAATTAATTCATTTTGATTATTGATTTTAGATAACTGGAGTGAAAAGGCAATCCGTAGAAAGACTACTGGAACTGGTAGGATGAGGTACTTGCGCAACGTTCCTCGCCGATTCAAGAGCGGTTTTAGAGAGGGTAATTAATCTTTTTCTAGCTTAATATGTTCGATTTAGTTTCTTAAGATTCAGTTTAGATCCTTAATCATTGATATGTTGTGGTTTTTTTTAATACAGGAACTGAAGCTGCACCTAGGAAGAAGGGAGCTGCGTCTGCTTAA

Protein sequence

>MS.gene85108.t1

MGKGTGSFGKRRNKTHTLCVRCGRRSFHLQKSRCSACAYPAARVRKYNWSEKAIRRKTTGTGRMRYLRNVPRRFKSGFREGTEAAPRKKGAASA