AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24930.t1 MTR_0147s0070 100.000 95 0 0 1 95 1 95 3.99e-65 191
MS.gene24930.t1 MTR_2g101900 96.809 94 3 0 1 94 1 94 1.39e-62 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24930.t1 AT1G52300 89.362 94 10 0 1 94 1 94 1.65e-57 172
MS.gene24930.t1 AT3G16080 88.298 94 11 0 1 94 1 94 4.05e-57 171
MS.gene24930.t1 AT1G15250 87.368 95 12 0 1 95 1 95 7.25e-57 170
MS.gene24930.t1 AT1G15250 87.368 95 12 0 1 95 1 95 7.25e-57 170
MS.gene24930.t1 AT3G16080 93.333 45 3 0 1 45 1 45 9.42e-25 89.0

Find 28 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCAGCTGCTCCCTTGTTCC+TGG 0.247813 4.3:+84433311 None:intergenic
ACTGGAGTGAGAAGGCTATT+AGG 0.322324 4.3:-84434332 MS.gene24930:CDS
ATTGTGTGATTTCAGATAAC+TGG 0.324608 4.3:-84434350 MS.gene24930:intron
GGTAAGGGAACAGGCAGTTT+TGG 0.365234 4.3:-84434643 MS.gene24930:CDS
ATGCTGAGCAGCGACTCTTC+TGG 0.400063 4.3:+84434560 None:intergenic
CATTTCCTGACACGTGCAGC+TGG 0.423824 4.3:+84434529 None:intergenic
AGACCCACACACTCTGTGTT+AGG 0.433282 4.3:-84434606 MS.gene24930:CDS
GCAGCTGCTCCCTTGTTCCT+GGG 0.437974 4.3:+84433312 None:intergenic
AGGAGAAAGACCACTGGAAC+TGG 0.467139 4.3:-84434312 MS.gene24930:CDS
CTTACCCAGCTGCACGTGTC+AGG 0.470321 4.3:-84434534 MS.gene24930:CDS
TGAAGCTGCACCCAGGAACA+AGG 0.550050 4.3:-84433322 MS.gene24930:CDS
AGCAGCGACTCTTCTGGAGA+TGG 0.555977 4.3:+84434566 None:intergenic
CACACCTAACACAGAGTGTG+TGG 0.573805 4.3:+84434602 None:intergenic
TTCAAGAGTGGATTCAGAGA+AGG 0.583941 4.3:-84434255 MS.gene24930:intron
TTCAGATAACTGGAGTGAGA+AGG 0.609846 4.3:-84434340 MS.gene24930:intron
ATTTCCTGACACGTGCAGCT+GGG 0.610760 4.3:+84434530 None:intergenic
CCACTGGAACTGGTAGGATG+AGG 0.619264 4.3:-84434302 MS.gene24930:CDS
GCTATTAGGAGAAAGACCAC+TGG 0.627964 4.3:-84434318 MS.gene24930:CDS
CTGGTAGGATGAGGTACTTG+AGG 0.643276 4.3:-84434293 MS.gene24930:CDS
CACACACTCTGTGTTAGGTG+TGG 0.644280 4.3:-84434601 MS.gene24930:CDS
GAAAGACCACTGGAACTGGT+AGG 0.652178 4.3:-84434308 MS.gene24930:CDS
GAAGCTGCACCCAGGAACAA+GGG 0.652652 4.3:-84433321 MS.gene24930:CDS
TCTGGAGATGGAAACTGCGA+CGG 0.655757 4.3:+84434578 None:intergenic
CCTCATCCTACCAGTTCCAG+TGG 0.656605 4.3:+84434302 None:intergenic
CAGGTACTGAAGCTGCACCC+AGG 0.673244 4.3:-84433329 MS.gene24930:CDS
ACACCTAACACAGAGTGTGT+GGG 0.685746 4.3:+84434603 None:intergenic
GTGCCTCGCAGATTCAAGAG+TGG 0.690811 4.3:-84434267 MS.gene24930:CDS
AATCCACTCTTGAATCTGCG+AGG 0.695196 4.3:+84434264 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CGTTAAATTATTTTTATATA+TGG - chr4.3:84434238-84434257 MS.gene24930:intron 10.0%
!! TTGAAAAAACCTAATAAAAA+TGG - chr4.3:84434308-84434327 MS.gene24930:CDS 15.0%
!! TTTGTTGATATGATTATTAT+TGG - chr4.3:84434684-84434703 MS.gene24930:intron 15.0%
!!! AAAATTTGATTTTTGGATTT+GGG - chr4.3:84433661-84433680 MS.gene24930:intron 15.0%
!!! TTCTAGAATTATCATTTTTT+TGG - chr4.3:84433978-84433997 MS.gene24930:intron 15.0%
!!! TTTTTTCGATTTTAGAAAAT+GGG - chr4.3:84433544-84433563 MS.gene24930:intron 15.0%
!!! TTTTTTTCGATTTTAGAAAA+TGG - chr4.3:84433543-84433562 MS.gene24930:intron 15.0%
!! ATTTAATTGATTGATCAATC+AGG - chr4.3:84433949-84433968 MS.gene24930:intron 20.0%
!! TTAGTAGATAAGTTCAAATA+AGG - chr4.3:84434366-84434385 MS.gene24930:intron 20.0%
!!! ATTATTGAATGATTTTTGCA+GGG - chr4.3:84433378-84433397 MS.gene24930:intron 20.0%
!!! ATTTTGACATGTTTTACTAT+AGG - chr4.3:84434494-84434513 MS.gene24930:intron 20.0%
!!! CAAAATTTGATTTTTGGATT+TGG - chr4.3:84433660-84433679 MS.gene24930:intron 20.0%
!!! GTTTATTGATTTTCGAAAAT+GGG - chr4.3:84433630-84433649 MS.gene24930:intron 20.0%
!!! TGTTTATTGATTTTCGAAAA+TGG - chr4.3:84433629-84433648 MS.gene24930:intron 20.0%
!!! TTGTGTCAAAATTTGATTTT+TGG - chr4.3:84433654-84433673 MS.gene24930:intron 20.0%
! ATTCACATAAAACAGAGATT+TGG + chr4.3:84434538-84434557 None:intergenic 25.0%
!! GATTATTGAATGATTTTTGC+AGG - chr4.3:84433377-84433396 MS.gene24930:intron 25.0%
!! TGATTATTATTGGTTAAACC+AGG - chr4.3:84434694-84434713 MS.gene24930:intron 25.0%
!!! AGCTTATGACCATTTTTATT+AGG + chr4.3:84434320-84434339 None:intergenic 25.0%
!!! ATTTTGGTGTCTTTAGATTT+AGG - chr4.3:84433584-84433603 MS.gene24930:intron 25.0%
!!! GTTACTAAATGAGTTGATTT+AGG - chr4.3:84434089-84434108 MS.gene24930:intron 25.0%
!!! TTCTGAGTTGTTATTTTTTC+TGG - chr4.3:84434653-84434672 MS.gene24930:intron 25.0%
!!! TTGGTGTTTAAATTCGATTT+TGG - chr4.3:84433568-84433587 MS.gene24930:intron 25.0%
!!! TTTTGGTGTCTTTAGATTTA+GGG - chr4.3:84433585-84433604 MS.gene24930:intron 25.0%
AATTTAGATCAGTTGGATCT+TGG - chr4.3:84434406-84434425 MS.gene24930:intron 30.0%
ATTGTGTGATTTCAGATAAC+TGG - chr4.3:84433692-84433711 MS.gene24930:intron 30.0%
TACACCCAAATAAAGAGATT+TGG + chr4.3:84434119-84434138 None:intergenic 30.0%
! AGAGCCAAATCTCTTTATTT+GGG - chr4.3:84434112-84434131 MS.gene24930:intron 30.0%
! AGGTTCTAATTTAGATCAGT+TGG - chr4.3:84434399-84434418 MS.gene24930:intron 30.0%
! CTTTTGTTAGCATGATTGTA+TGG - chr4.3:84434614-84434633 MS.gene24930:CDS 30.0%
! TAGATTTAGGGTTTAAGTTC+AGG - chr4.3:84433597-84433616 MS.gene24930:intron 30.0%
!! TCGATTTTAGAAAATGGGTT+TGG - chr4.3:84433549-84433568 MS.gene24930:intron 30.0%
GAGAGCCAAATCTCTTTATT+TGG - chr4.3:84434111-84434130 MS.gene24930:intron 35.0%
GATCTAAATTAGAACCTGCA+TGG + chr4.3:84434396-84434415 None:intergenic 35.0%
GTGCTAGAAAAACTAAACAC+AGG + chr4.3:84433835-84433854 None:intergenic 35.0%
TCAGACACTGTAATGAAACT+GGG + chr4.3:84433354-84433373 None:intergenic 35.0%
TTCAGACACTGTAATGAAAC+TGG + chr4.3:84433355-84433374 None:intergenic 35.0%
! GAATGATTTTTGCAGGGTAA+GGG - chr4.3:84433384-84433403 MS.gene24930:intron 35.0%
! TAGGGTTTAAGTTCAGGAAA+TGG - chr4.3:84433603-84433622 MS.gene24930:intron 35.0%
! TGAATGATTTTTGCAGGGTA+AGG - chr4.3:84433383-84433402 MS.gene24930:intron 35.0%
!! AGTTGGATCTTGGTTCTTTT+AGG - chr4.3:84434416-84434435 MS.gene24930:intron 35.0%
!!! TTGGGTGTAGCAACTTTTTT+GGG - chr4.3:84434130-84434149 MS.gene24930:intron 35.0%
!!! TTTGGGTGTAGCAACTTTTT+TGG - chr4.3:84434129-84434148 MS.gene24930:intron 35.0%
CAGACACTGTAATGAAACTG+GGG + chr4.3:84433353-84433372 None:intergenic 40.0%
TCAAATAAGGCAATCCATGC+AGG - chr4.3:84434379-84434398 MS.gene24930:intron 40.0%
TTCAGATAACTGGAGTGAGA+AGG - chr4.3:84433702-84433721 MS.gene24930:intron 40.0%
! CTGTAATGAAACTGGGGTTT+TGG + chr4.3:84433347-84433366 None:intergenic 40.0%
! TTCAAGAGTGGATTCAGAGA+AGG - chr4.3:84433787-84433806 MS.gene24930:intron 40.0%
AATCCACTCTTGAATCTGCG+AGG + chr4.3:84433781-84433800 None:intergenic 45.0%
ACACCTAACACAGAGTGTGT+GGG + chr4.3:84433442-84433461 None:intergenic 45.0%
ACTGGAGTGAGAAGGCTATT+AGG - chr4.3:84433710-84433729 MS.gene24930:intron 45.0%
GCTATTAGGAGAAAGACCAC+TGG - chr4.3:84433724-84433743 MS.gene24930:intron 45.0%
! TTTTTGCAGGGTAAGGGAAC+AGG - chr4.3:84433390-84433409 MS.gene24930:intron 45.0%
!! CAGGCAGTTTTGGTAAGAGA+AGG - chr4.3:84433409-84433428 MS.gene24930:intron 45.0%
!!! AAACTGGGGTTTTGGATCGA+AGG + chr4.3:84433339-84433358 None:intergenic 45.0%
AGACCCACACACTCTGTGTT+AGG - chr4.3:84433436-84433455 MS.gene24930:intron 50.0%
AGGAGAAAGACCACTGGAAC+TGG - chr4.3:84433730-84433749 MS.gene24930:intron 50.0%
ATTTCCTGACACGTGCAGCT+GGG + chr4.3:84433515-84433534 None:intergenic 50.0%
CACACACTCTGTGTTAGGTG+TGG - chr4.3:84433441-84433460 MS.gene24930:intron 50.0%
CACACCTAACACAGAGTGTG+TGG + chr4.3:84433443-84433462 None:intergenic 50.0%
GAAAGACCACTGGAACTGGT+AGG - chr4.3:84433734-84433753 MS.gene24930:intron 50.0%
TCTGGAGATGGAAACTGCGA+CGG + chr4.3:84433467-84433486 None:intergenic 50.0%
! CTGGTAGGATGAGGTACTTG+AGG - chr4.3:84433749-84433768 MS.gene24930:intron 50.0%
! GGTAAGGGAACAGGCAGTTT+TGG - chr4.3:84433399-84433418 MS.gene24930:intron 50.0%
ATGCTGAGCAGCGACTCTTC+TGG + chr4.3:84433485-84433504 None:intergenic 55.0%
CATTTCCTGACACGTGCAGC+TGG + chr4.3:84433516-84433535 None:intergenic 55.0%
CCACTGGAACTGGTAGGATG+AGG - chr4.3:84433740-84433759 MS.gene24930:intron 55.0%
CCTCATCCTACCAGTTCCAG+TGG + chr4.3:84433743-84433762 None:intergenic 55.0%
GAAGCTGCACCCAGGAACAA+GGG - chr4.3:84434721-84434740 MS.gene24930:intron 55.0%
GTGCCTCGCAGATTCAAGAG+TGG - chr4.3:84433775-84433794 MS.gene24930:intron 55.0%
TGAAGCTGCACCCAGGAACA+AGG - chr4.3:84434720-84434739 MS.gene24930:intron 55.0%
! AGCAGCGACTCTTCTGGAGA+TGG + chr4.3:84433479-84433498 None:intergenic 55.0%
AGCAGCTGCTCCCTTGTTCC+TGG + chr4.3:84434734-84434753 None:intergenic 60.0%
CTGGGTGCAGCTTCAGTACC+TGG + chr4.3:84434715-84434734 None:intergenic 60.0%
CTTACCCAGCTGCACGTGTC+AGG - chr4.3:84433508-84433527 MS.gene24930:intron 60.0%
GCAGCTGCTCCCTTGTTCCT+GGG + chr4.3:84434733-84434752 None:intergenic 60.0%
!! CAGGTACTGAAGCTGCACCC+AGG - chr4.3:84434713-84434732 MS.gene24930:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 84433302 84434762 84433302 ID=MS.gene24930
chr4.3 mRNA 84433302 84434762 84433302 ID=MS.gene24930.t1;Parent=MS.gene24930
chr4.3 exon 84434760 84434762 84434760 ID=MS.gene24930.t1.exon1;Parent=MS.gene24930.t1
chr4.3 CDS 84434760 84434762 84434760 ID=cds.MS.gene24930.t1;Parent=MS.gene24930.t1
chr4.3 exon 84434530 84434665 84434530 ID=MS.gene24930.t1.exon2;Parent=MS.gene24930.t1
chr4.3 CDS 84434530 84434665 84434530 ID=cds.MS.gene24930.t1;Parent=MS.gene24930.t1
chr4.3 exon 84434256 84434357 84434256 ID=MS.gene24930.t1.exon3;Parent=MS.gene24930.t1
chr4.3 CDS 84434256 84434357 84434256 ID=cds.MS.gene24930.t1;Parent=MS.gene24930.t1
chr4.3 exon 84433302 84433348 84433302 ID=MS.gene24930.t1.exon4;Parent=MS.gene24930.t1
chr4.3 CDS 84433302 84433348 84433302 ID=cds.MS.gene24930.t1;Parent=MS.gene24930.t1
Gene Sequence

>MS.gene24930

ATGGTATTTTGCATTATCTCTTCTCTCTCTCTCTCCTTCGATCCAAAACCCCAGTTTCATTACAGTGTCTGAATCGATTATTGAATGATTTTTGCAGGGTAAGGGAACAGGCAGTTTTGGTAAGAGAAGGAACAAGACCCACACACTCTGTGTTAGGTGTGGCCGTCGCAGTTTCCATCTCCAGAAGAGTCGCTGCTCAGCATGTGCTTACCCAGCTGCACGTGTCAGGAAATGTAAGTTTTTTTTTTCGATTTTAGAAAATGGGTTTGGTGTTTAAATTCGATTTTGGTGTCTTTAGATTTAGGGTTTAAGTTCAGGAAATGGAAGTGTTTATTGATTTTCGAAAATGGGTTTGTGTCAAAATTTGATTTTTGGATTTGGGCGTTTTGTATTGTGTGATTTCAGATAACTGGAGTGAGAAGGCTATTAGGAGAAAGACCACTGGAACTGGTAGGATGAGGTACTTGAGGAACGTGCCTCGCAGATTCAAGAGTGGATTCAGAGAAGGTAATTCTTTCTTTAATCAATTTCCTGTGTTTAGTTTTTCTAGCACGTGCAATGCTTTAAATATATACTAATTAAGCAATAATTTATGAATCAATAGATGAATAACTGTTAAGTTCATAAAGTAAAATGTTTTGTATTTGATTTAATTGATTGATCAATCAGGATATTTTTCTAGAATTATCATTTTTTTGGTACGCTAGAATGAGCAACTTTACAATATGATTTAATTGATAATATCATTATCAATAGATGAATGACTGTTAAGCTTAAACTTCTGTGTGTTACTAAATGAGTTGATTTAGGAGAGCCAAATCTCTTTATTTGGGTGTAGCAACTTTTTTGGGTTTGTGTTTTGTTAAAATGATTACAATGACTGCTGCTCATGTATAAGCTTGCATATGCTATTTTTAACAGCAAAAGAAAAAGTAACGTTAAATTATTTTTATATATGGTATAAGTTGTTTTATAAGCTATATTTAAGAAACTTGTGAAAAATAAGTTGAAAAAACCTAATAAAAATGGTCATAAGCTGTTTTCATAAGTTCTCACAACTTATGTTAGTAGATAAGTTCAAATAAGGCAATCCATGCAGGTTCTAATTTAGATCAGTTGGATCTTGGTTCTTTTAGGTGTGTTATTCTGTTTGTATTGAAACATTATTATGATTATAATACGTTAAATGTTAATTTTGACATGTTTTACTATAGGTATGTTATTTTATAAGCGCCAAATCTCTGTTTTATGTGAATTATTTGTTTGTTTGTGTATTTTGCTAATGTGAGCTCTTTTAGTTCTGTTTTTTTAGCTTTTGTTAGCATGATTGTATGGAAGTAGTTAAATCACTTTCTGAGTTGTTATTTTTTCTGGATTATTTTTTTGTTGATATGATTATTATTGGTTAAACCAGGTACTGAAGCTGCACCCAGGAACAAGGGAGCAGCTGCTTCTACCTAA

Protein sequence

>MS.gene24930.t1

MGKGTGSFGKRRNKTHTLCVRCGRRSFHLQKSRCSACAYPAARVRKYNWSEKAIRRKTTGTGRMRYLRNVPRRFKSGFREGTEAAPRNKGAAAST