Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068255.t1 | XP_013442541.1 | 100 | 95 | 0 | 0 | 1 | 95 | 1 | 95 | 6.50E-46 | 193 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068255.t1 | Q43292 | 89.4 | 94 | 10 | 0 | 1 | 94 | 1 | 94 | 2.2e-42 | 172.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068255.t1 | G7JJE2 | 100.0 | 95 | 0 | 0 | 1 | 95 | 1 | 95 | 4.7e-46 | 193.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene068255 | 0.856025 | 4.25E-62 | -1.69E-46 |
| MS.gene050752 | MS.gene068255 | 0.818789 | 1.63E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene068255 | 0.820844 | 5.53E-53 | -1.69E-46 |
| MS.gene050916 | MS.gene068255 | 0.806798 | 6.91E-50 | -1.69E-46 |
| MS.gene050955 | MS.gene068255 | 0.802497 | 5.46E-49 | -1.69E-46 |
| MS.gene051330 | MS.gene068255 | 0.813268 | 2.79E-51 | -1.69E-46 |
| MS.gene051746 | MS.gene068255 | 0.805935 | 1.05E-49 | -1.69E-46 |
| MS.gene051850 | MS.gene068255 | 0.80917 | 2.16E-50 | -1.69E-46 |
| MS.gene051903 | MS.gene068255 | 0.849825 | 2.52E-60 | -1.69E-46 |
| MS.gene052947 | MS.gene068255 | 0.840784 | 7.06E-58 | -1.69E-46 |
| MS.gene053952 | MS.gene068255 | 0.819365 | 1.21E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene068255 | 0.81758 | 3.06E-52 | -1.69E-46 |
| MS.gene054937 | MS.gene068255 | 0.873339 | 1.59E-67 | -1.69E-46 |
| MS.gene056075 | MS.gene068255 | 0.870422 | 1.48E-66 | -1.69E-46 |
| MS.gene056917 | MS.gene068255 | 0.811483 | 6.84E-51 | -1.69E-46 |
| MS.gene057094 | MS.gene068255 | 0.826155 | 3.17E-54 | -1.69E-46 |
| MS.gene057206 | MS.gene068255 | 0.801751 | 7.77E-49 | -1.69E-46 |
| MS.gene059227 | MS.gene068255 | 0.819127 | 1.37E-52 | -1.69E-46 |
| MS.gene06001 | MS.gene068255 | 0.84552 | 3.86E-59 | -1.69E-46 |
| MS.gene061145 | MS.gene068255 | 0.846031 | 2.80E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene85108 | MS.gene068255 | PPI |
| MS.gene052947 | MS.gene068255 | PPI |
| MS.gene33384 | MS.gene068255 | PPI |
| MS.gene47819 | MS.gene068255 | PPI |
| MS.gene068255 | MS.gene85110 | PPI |
| MS.gene24930 | MS.gene068255 | PPI |
| MS.gene36310 | MS.gene068255 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068255.t1 | MTR_0147s0070 | 100.000 | 95 | 0 | 0 | 1 | 95 | 1 | 95 | 3.99e-65 | 191 |
| MS.gene068255.t1 | MTR_2g101900 | 96.809 | 94 | 3 | 0 | 1 | 94 | 1 | 94 | 1.39e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068255.t1 | AT1G52300 | 89.362 | 94 | 10 | 0 | 1 | 94 | 1 | 94 | 1.65e-57 | 172 |
| MS.gene068255.t1 | AT3G16080 | 88.298 | 94 | 11 | 0 | 1 | 94 | 1 | 94 | 4.05e-57 | 171 |
| MS.gene068255.t1 | AT1G15250 | 87.368 | 95 | 12 | 0 | 1 | 95 | 1 | 95 | 7.25e-57 | 170 |
| MS.gene068255.t1 | AT1G15250 | 87.368 | 95 | 12 | 0 | 1 | 95 | 1 | 95 | 7.25e-57 | 170 |
| MS.gene068255.t1 | AT3G16080 | 93.333 | 45 | 3 | 0 | 1 | 45 | 1 | 45 | 9.42e-25 | 89.0 |
Find 27 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAGCTGCTCCCTTGTTCC+TGG | 0.247813 | 4.4:+87355419 | None:intergenic |
| ACTGGAGTGAGAAGGCTATT+AGG | 0.322324 | 4.4:-87356481 | MS.gene068255:CDS |
| ATTGTGTGATTTCAGATAAC+TGG | 0.324608 | 4.4:-87356499 | MS.gene068255:intron |
| GGTAAGGGAACAGGCAGTTT+TGG | 0.365234 | 4.4:-87356768 | MS.gene068255:CDS |
| ATGCTGAGCAGCGACTCTTC+TGG | 0.400063 | 4.4:+87356685 | None:intergenic |
| AGACCCACACACTTTGTGTT+AGG | 0.406757 | 4.4:-87356731 | MS.gene068255:CDS |
| GCAGCTGCTCCCTTGTTCCT+GGG | 0.437974 | 4.4:+87355420 | None:intergenic |
| CTTATCCAGCTGCACGTGTC+AGG | 0.448216 | 4.4:-87356659 | MS.gene068255:CDS |
| AGGAGAAAGACCACTGGAAC+TGG | 0.467139 | 4.4:-87356461 | MS.gene068255:CDS |
| CATTTCCTGACACGTGCAGC+TGG | 0.473916 | 4.4:+87356654 | None:intergenic |
| TGAAGCTGCACCCAGGAACA+AGG | 0.550050 | 4.4:-87355430 | MS.gene068255:CDS |
| AGCAGCGACTCTTCTGGAGA+TGG | 0.555977 | 4.4:+87356691 | None:intergenic |
| CACACCTAACACAAAGTGTG+TGG | 0.581535 | 4.4:+87356727 | None:intergenic |
| TTCAAGAGTGGATTCAGAGA+AGG | 0.583941 | 4.4:-87356404 | MS.gene068255:intron |
| CACACACTTTGTGTTAGGTG+TGG | 0.595008 | 4.4:-87356726 | MS.gene068255:CDS |
| TTCAGATAACTGGAGTGAGA+AGG | 0.609846 | 4.4:-87356489 | MS.gene068255:intron |
| CCACTGGAACTGGTAGGATG+AGG | 0.619264 | 4.4:-87356451 | MS.gene068255:CDS |
| GCTATTAGGAGAAAGACCAC+TGG | 0.627964 | 4.4:-87356467 | MS.gene068255:CDS |
| CTGGTAGGATGAGGTACTTG+AGG | 0.643276 | 4.4:-87356442 | MS.gene068255:CDS |
| GAAAGACCACTGGAACTGGT+AGG | 0.652178 | 4.4:-87356457 | MS.gene068255:CDS |
| GAAGCTGCACCCAGGAACAA+GGG | 0.652652 | 4.4:-87355429 | MS.gene068255:CDS |
| TCTGGAGATGGAAACTGCGA+CGG | 0.655757 | 4.4:+87356703 | None:intergenic |
| CCTCATCCTACCAGTTCCAG+TGG | 0.656605 | 4.4:+87356451 | None:intergenic |
| ACACCTAACACAAAGTGTGT+GGG | 0.666393 | 4.4:+87356728 | None:intergenic |
| CAGGTACTGAAGCTGCACCC+AGG | 0.673244 | 4.4:-87355437 | MS.gene068255:CDS |
| GTGCCTCGCAGATTCAAGAG+TGG | 0.690811 | 4.4:-87356416 | MS.gene068255:CDS |
| AATCCACTCTTGAATCTGCG+AGG | 0.695196 | 4.4:+87356413 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATTTGATTTTTGAATTT+GGG | - | chr4.4:87355747-87355766 | MS.gene068255:intron | 10.0% |
| !!! | CGTTAAATTATTTTTATATA+TGG | - | chr4.4:87356366-87356385 | MS.gene068255:intron | 10.0% |
| !! | TTGAAAAAACCTAATAAAAA+TGG | - | chr4.4:87356436-87356455 | MS.gene068255:CDS | 15.0% |
| !!! | ATTTTTACATGTTTTACTAT+AGG | - | chr4.4:87356621-87356640 | MS.gene068255:intron | 15.0% |
| !!! | ATTTTTGTGTTTTTAGATTT+AGG | - | chr4.4:87355670-87355689 | MS.gene068255:intron | 15.0% |
| !!! | CAAAATTTGATTTTTGAATT+TGG | - | chr4.4:87355746-87355765 | MS.gene068255:intron | 15.0% |
| !!! | TTCTAGAATTATCATTTTTT+TGG | - | chr4.4:87356065-87356084 | MS.gene068255:intron | 15.0% |
| !!! | TTTTTGTGTTTTTAGATTTA+GGG | - | chr4.4:87355671-87355690 | MS.gene068255:intron | 15.0% |
| !! | ATTTAATTGATTGATCAATC+AGG | - | chr4.4:87356035-87356054 | MS.gene068255:intron | 20.0% |
| !! | TGATTATTATTGTTTAAACC+AGG | - | chr4.4:87356821-87356840 | MS.gene068255:intron | 20.0% |
| !! | TTTAATTGATTGATCAATCA+GGG | - | chr4.4:87356036-87356055 | MS.gene068255:intron | 20.0% |
| !!! | ATTATTGAATGATTTTTGCA+GGG | - | chr4.4:87355488-87355507 | MS.gene068255:intron | 20.0% |
| !!! | GTTTTTTGATTTTCGAAAAT+GGG | - | chr4.4:87355716-87355735 | MS.gene068255:intron | 20.0% |
| !!! | TGTTTTTTGATTTTCGAAAA+TGG | - | chr4.4:87355715-87355734 | MS.gene068255:intron | 20.0% |
| ! | ATTCACTTAAAACAGAGATT+TGG | + | chr4.4:87356665-87356684 | None:intergenic | 25.0% |
| ! | TACACCTAAATAAAGAGATT+TGG | + | chr4.4:87356206-87356225 | None:intergenic | 25.0% |
| ! | TTAGTAGATAAGTTCAACTA+AGG | - | chr4.4:87356493-87356512 | MS.gene068255:intron | 25.0% |
| !! | GATTATTGAATGATTTTTGC+AGG | - | chr4.4:87355487-87355506 | MS.gene068255:intron | 25.0% |
| !!! | AGCTTATGACCATTTTTATT+AGG | + | chr4.4:87356448-87356467 | None:intergenic | 25.0% |
| !!! | TTCTGAGTTGTTATTTTTTC+TGG | - | chr4.4:87356780-87356799 | MS.gene068255:intron | 25.0% |
| ATTGTGTGATTTCAGATAAC+TGG | - | chr4.4:87355778-87355797 | MS.gene068255:intron | 30.0% | |
| ! | AGAGCCAAATCTCTTTATTT+AGG | - | chr4.4:87356199-87356218 | MS.gene068255:intron | 30.0% |
| ! | CTTTTGTTAGCATGATTGTA+TGG | - | chr4.4:87356741-87356760 | MS.gene068255:CDS | 30.0% |
| ! | TAGATTTAGGGTTTAAGTTC+AGG | - | chr4.4:87355683-87355702 | MS.gene068255:intron | 30.0% |
| !! | GTTACTGAATGAGTTGATTT+AGG | - | chr4.4:87356176-87356195 | MS.gene068255:intron | 30.0% |
| ATAAGCTGAATATCCAATGC+AGG | + | chr4.4:87356279-87356298 | None:intergenic | 35.0% | |
| GTGCTAGAAAAACTAAACAC+AGG | + | chr4.4:87355921-87355940 | None:intergenic | 35.0% | |
| TAAGCTGAATATCCAATGCA+GGG | + | chr4.4:87356278-87356297 | None:intergenic | 35.0% | |
| TCAGACACTGTAATGAAACT+GGG | + | chr4.4:87355464-87355483 | None:intergenic | 35.0% | |
| TTAGGTGTAGCAACTTTCTT+GGG | - | chr4.4:87356217-87356236 | MS.gene068255:intron | 35.0% | |
| TTCAGACACTGTAATGAAAC+TGG | + | chr4.4:87355465-87355484 | None:intergenic | 35.0% | |
| TTTAGGTGTAGCAACTTTCT+TGG | - | chr4.4:87356216-87356235 | MS.gene068255:intron | 35.0% | |
| ! | AATTTAGCTCAGTTGGATCT+TGG | - | chr4.4:87356533-87356552 | MS.gene068255:intron | 35.0% |
| ! | AGGTTCTAATTTAGCTCAGT+TGG | - | chr4.4:87356526-87356545 | MS.gene068255:intron | 35.0% |
| ! | GAATGATTTTTGCAGGGTAA+GGG | - | chr4.4:87355494-87355513 | MS.gene068255:intron | 35.0% |
| ! | TAGGGTTTAAGTTCAGGAAA+TGG | - | chr4.4:87355689-87355708 | MS.gene068255:intron | 35.0% |
| ! | TGAATGATTTTTGCAGGGTA+AGG | - | chr4.4:87355493-87355512 | MS.gene068255:intron | 35.0% |
| !! | AGTTGGATCTTGGTTCTTTT+AGG | - | chr4.4:87356543-87356562 | MS.gene068255:intron | 35.0% |
| ACACCTAACACAAAGTGTGT+GGG | + | chr4.4:87355552-87355571 | None:intergenic | 40.0% | |
| CAGACACTGTAATGAAACTG+GGG | + | chr4.4:87355463-87355482 | None:intergenic | 40.0% | |
| GAGCTAAATTAGAACCTGCA+TGG | + | chr4.4:87356523-87356542 | None:intergenic | 40.0% | |
| TTCAGATAACTGGAGTGAGA+AGG | - | chr4.4:87355788-87355807 | MS.gene068255:intron | 40.0% | |
| ! | CTGTAATGAAACTGGGGTTT+TGG | + | chr4.4:87355457-87355476 | None:intergenic | 40.0% |
| ! | TTCAAGAGTGGATTCAGAGA+AGG | - | chr4.4:87355873-87355892 | MS.gene068255:intron | 40.0% |
| AATCCACTCTTGAATCTGCG+AGG | + | chr4.4:87355867-87355886 | None:intergenic | 45.0% | |
| ACTGGAGTGAGAAGGCTATT+AGG | - | chr4.4:87355796-87355815 | MS.gene068255:intron | 45.0% | |
| AGACCCACACACTTTGTGTT+AGG | - | chr4.4:87355546-87355565 | MS.gene068255:intron | 45.0% | |
| CAATGATCAAGTCCCTGCAT+TGG | - | chr4.4:87356263-87356282 | MS.gene068255:intron | 45.0% | |
| CACACACTTTGTGTTAGGTG+TGG | - | chr4.4:87355551-87355570 | MS.gene068255:intron | 45.0% | |
| CACACCTAACACAAAGTGTG+TGG | + | chr4.4:87355553-87355572 | None:intergenic | 45.0% | |
| GCTATTAGGAGAAAGACCAC+TGG | - | chr4.4:87355810-87355829 | MS.gene068255:intron | 45.0% | |
| TCAACTAAGGCAATCCATGC+AGG | - | chr4.4:87356506-87356525 | MS.gene068255:intron | 45.0% | |
| ! | TTTTTGCAGGGTAAGGGAAC+AGG | - | chr4.4:87355500-87355519 | MS.gene068255:intron | 45.0% |
| !! | CAGGCAGTTTTGGTAAGAGA+AGG | - | chr4.4:87355519-87355538 | MS.gene068255:intron | 45.0% |
| !!! | AAACTGGGGTTTTGGATCGA+AGG | + | chr4.4:87355449-87355468 | None:intergenic | 45.0% |
| AGGAGAAAGACCACTGGAAC+TGG | - | chr4.4:87355816-87355835 | MS.gene068255:intron | 50.0% | |
| GAAAGACCACTGGAACTGGT+AGG | - | chr4.4:87355820-87355839 | MS.gene068255:intron | 50.0% | |
| TCTGGAGATGGAAACTGCGA+CGG | + | chr4.4:87355577-87355596 | None:intergenic | 50.0% | |
| ! | CTGGTAGGATGAGGTACTTG+AGG | - | chr4.4:87355835-87355854 | MS.gene068255:intron | 50.0% |
| ! | GGTAAGGGAACAGGCAGTTT+TGG | - | chr4.4:87355509-87355528 | MS.gene068255:intron | 50.0% |
| ATGCTGAGCAGCGACTCTTC+TGG | + | chr4.4:87355595-87355614 | None:intergenic | 55.0% | |
| CATTTCCTGACACGTGCAGC+TGG | + | chr4.4:87355626-87355645 | None:intergenic | 55.0% | |
| CCACTGGAACTGGTAGGATG+AGG | - | chr4.4:87355826-87355845 | MS.gene068255:intron | 55.0% | |
| CCTCATCCTACCAGTTCCAG+TGG | + | chr4.4:87355829-87355848 | None:intergenic | 55.0% | |
| CTTATCCAGCTGCACGTGTC+AGG | - | chr4.4:87355618-87355637 | MS.gene068255:intron | 55.0% | |
| GAAGCTGCACCCAGGAACAA+GGG | - | chr4.4:87356848-87356867 | MS.gene068255:intron | 55.0% | |
| GTGCCTCGCAGATTCAAGAG+TGG | - | chr4.4:87355861-87355880 | MS.gene068255:intron | 55.0% | |
| TGAAGCTGCACCCAGGAACA+AGG | - | chr4.4:87356847-87356866 | MS.gene068255:intron | 55.0% | |
| ! | AGCAGCGACTCTTCTGGAGA+TGG | + | chr4.4:87355589-87355608 | None:intergenic | 55.0% |
| AGCAGCTGCTCCCTTGTTCC+TGG | + | chr4.4:87356861-87356880 | None:intergenic | 60.0% | |
| CTGGGTGCAGCTTCAGTACC+TGG | + | chr4.4:87356842-87356861 | None:intergenic | 60.0% | |
| GCAGCTGCTCCCTTGTTCCT+GGG | + | chr4.4:87356860-87356879 | None:intergenic | 60.0% | |
| !! | CAGGTACTGAAGCTGCACCC+AGG | - | chr4.4:87356840-87356859 | MS.gene068255:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 87355410 | 87356889 | 87355410 | ID=MS.gene068255 |
| chr4.4 | mRNA | 87355410 | 87356889 | 87355410 | ID=MS.gene068255.t1;Parent=MS.gene068255 |
| chr4.4 | exon | 87356887 | 87356889 | 87356887 | ID=MS.gene068255.t1.exon1;Parent=MS.gene068255.t1 |
| chr4.4 | CDS | 87356887 | 87356889 | 87356887 | ID=cds.MS.gene068255.t1;Parent=MS.gene068255.t1 |
| chr4.4 | exon | 87356655 | 87356790 | 87356655 | ID=MS.gene068255.t1.exon2;Parent=MS.gene068255.t1 |
| chr4.4 | CDS | 87356655 | 87356790 | 87356655 | ID=cds.MS.gene068255.t1;Parent=MS.gene068255.t1 |
| chr4.4 | exon | 87356405 | 87356506 | 87356405 | ID=MS.gene068255.t1.exon3;Parent=MS.gene068255.t1 |
| chr4.4 | CDS | 87356405 | 87356506 | 87356405 | ID=cds.MS.gene068255.t1;Parent=MS.gene068255.t1 |
| chr4.4 | exon | 87355410 | 87355456 | 87355410 | ID=MS.gene068255.t1.exon4;Parent=MS.gene068255.t1 |
| chr4.4 | CDS | 87355410 | 87355456 | 87355410 | ID=cds.MS.gene068255.t1;Parent=MS.gene068255.t1 |
| Gene Sequence |
| Protein sequence |
