Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85110.t1 | XP_003597753.2 | 100 | 94 | 0 | 0 | 1 | 94 | 1 | 94 | 4.10E-45 | 190.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85110.t1 | Q8LEM8 | 88.3 | 94 | 11 | 0 | 1 | 94 | 1 | 94 | 6.3e-42 | 171.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85110.t1 | B7FGF1 | 100.0 | 94 | 0 | 0 | 1 | 94 | 1 | 94 | 3.0e-45 | 190.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene85110 | 0.831986 | 1.22E-55 | -1.69E-46 |
| MS.gene050290 | MS.gene85110 | 0.808701 | 2.72E-50 | -1.69E-46 |
| MS.gene051063 | MS.gene85110 | 0.80742 | 5.10E-50 | -1.69E-46 |
| MS.gene051330 | MS.gene85110 | 0.845212 | 4.68E-59 | -1.69E-46 |
| MS.gene051746 | MS.gene85110 | 0.823335 | 1.46E-53 | -1.69E-46 |
| MS.gene051748 | MS.gene85110 | 0.826874 | 2.13E-54 | -1.69E-46 |
| MS.gene051903 | MS.gene85110 | 0.864733 | 9.83E-65 | -1.69E-46 |
| MS.gene052947 | MS.gene85110 | 0.839848 | 1.24E-57 | -1.69E-46 |
| MS.gene053952 | MS.gene85110 | 0.826494 | 2.63E-54 | -1.69E-46 |
| MS.gene054937 | MS.gene85110 | 0.8381 | 3.52E-57 | -1.69E-46 |
| MS.gene056075 | MS.gene85110 | 0.800542 | 1.37E-48 | -1.69E-46 |
| MS.gene056601 | MS.gene85110 | 0.817569 | 3.08E-52 | -1.69E-46 |
| MS.gene056631 | MS.gene85110 | 0.854484 | 1.19E-61 | -1.69E-46 |
| MS.gene057094 | MS.gene85110 | 0.811457 | 6.94E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene85110 | 0.818975 | 1.48E-52 | -1.69E-46 |
| MS.gene058354 | MS.gene85110 | 0.813289 | 2.76E-51 | -1.69E-46 |
| MS.gene06001 | MS.gene85110 | 0.87426 | 7.76E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene85108 | MS.gene85110 | PPI |
| MS.gene052947 | MS.gene85110 | PPI |
| MS.gene33384 | MS.gene85110 | PPI |
| MS.gene47819 | MS.gene85110 | PPI |
| MS.gene068255 | MS.gene85110 | PPI |
| MS.gene24930 | MS.gene85110 | PPI |
| MS.gene36310 | MS.gene85110 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85110.t1 | MTR_2g101900 | 100.000 | 94 | 0 | 0 | 1 | 94 | 1 | 94 | 4.84e-64 | 188 |
| MS.gene85110.t1 | MTR_0147s0070 | 96.809 | 94 | 3 | 0 | 1 | 94 | 1 | 94 | 1.38e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85110.t1 | AT3G16080 | 88.298 | 94 | 11 | 0 | 1 | 94 | 1 | 94 | 7.27e-57 | 170 |
| MS.gene85110.t1 | AT1G52300 | 88.298 | 94 | 11 | 0 | 1 | 94 | 1 | 94 | 1.73e-56 | 169 |
| MS.gene85110.t1 | AT1G15250 | 87.234 | 94 | 12 | 0 | 1 | 94 | 1 | 94 | 9.08e-56 | 167 |
| MS.gene85110.t1 | AT1G15250 | 87.234 | 94 | 12 | 0 | 1 | 94 | 1 | 94 | 9.08e-56 | 167 |
| MS.gene85110.t1 | AT3G16080 | 93.333 | 45 | 3 | 0 | 1 | 45 | 1 | 45 | 9.58e-25 | 89.0 |
Find 26 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTAAAACCGCTCTTGAAT+CGG | 0.373242 | 2.4:-3020994 | None:intergenic |
| AAGCAGAGCATCTACTCTTC+TGG | 0.402579 | 2.4:-3020772 | None:intergenic |
| CTTACCCAGCTGCTCGTGTT+AGG | 0.412584 | 2.4:+3020798 | MS.gene85110:CDS |
| CATTTCCTAACACGAGCAGC+TGG | 0.431351 | 2.4:-3020803 | None:intergenic |
| GAATTGCAGGGTAAGGGAAC+TGG | 0.450011 | 2.4:+3020680 | MS.gene85110:intron |
| CGTAGAAAGACTACTGGAAC+TGG | 0.453204 | 2.4:+3020941 | MS.gene85110:CDS |
| TTTAGATAACTGGAGTGAAA+AGG | 0.467360 | 2.4:+3020913 | MS.gene85110:intron |
| CAGTTCCAGTAGTCTTTCTA+CGG | 0.470228 | 2.4:-3020940 | None:intergenic |
| GGTAAGGGAACTGGAAGTTT+CGG | 0.474527 | 2.4:+3020689 | MS.gene85110:CDS |
| AGACTCACACACTCTGTGTC+AGG | 0.478990 | 2.4:+3020726 | MS.gene85110:CDS |
| TGAAGCTGCACCTAGGAAGA+AGG | 0.512832 | 2.4:+3021122 | MS.gene85110:CDS |
| AGCATCTACTCTTCTGGAGA+TGG | 0.532321 | 2.4:-3020766 | None:intergenic |
| GCAATCCGTAGAAAGACTAC+TGG | 0.532707 | 2.4:+3020935 | MS.gene85110:CDS |
| GTGAATGAATTGCAGGGTAA+GGG | 0.538112 | 2.4:+3020674 | MS.gene85110:intron |
| GACGCAGCTCCCTTCTTCCT+AGG | 0.557312 | 2.4:-3021132 | None:intergenic |
| GAAGCTGCACCTAGGAAGAA+GGG | 0.598975 | 2.4:+3021123 | MS.gene85110:CDS |
| CACACACTCTGTGTCAGGTG+TGG | 0.600659 | 2.4:+3020731 | MS.gene85110:CDS |
| CTACTGGAACTGGTAGGATG+AGG | 0.613944 | 2.4:+3020951 | MS.gene85110:CDS |
| CTGGAAGTTTCGGTAAGAGA+AGG | 0.618981 | 2.4:+3020699 | MS.gene85110:CDS |
| CAGGAACTGAAGCTGCACCT+AGG | 0.634185 | 2.4:+3021115 | MS.gene85110:intron |
| ATTTCCTAACACGAGCAGCT+GGG | 0.638199 | 2.4:-3020802 | None:intergenic |
| GAAAGACTACTGGAACTGGT+AGG | 0.653500 | 2.4:+3020945 | MS.gene85110:CDS |
| TCTGGAGATGGAAACTGCGG+CGG | 0.656436 | 2.4:-3020754 | None:intergenic |
| TCTTCTGGAGATGGAAACTG+CGG | 0.675301 | 2.4:-3020757 | None:intergenic |
| GTTCCTCGCCGATTCAAGAG+CGG | 0.676604 | 2.4:+3020986 | MS.gene85110:CDS |
| AAACCGCTCTTGAATCGGCG+AGG | 0.683649 | 2.4:-3020989 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTAATTTGATTTGTAATTA+GGG | + | chr2.4:3020845-3020864 | MS.gene85110:intron | 15.0% |
| !!! | TGTTAATTTGATTTGTAATT+AGG | + | chr2.4:3020844-3020863 | MS.gene85110:intron | 15.0% |
| !!! | GATTATTGATTTTAGATAAC+TGG | + | chr2.4:3020903-3020922 | MS.gene85110:intron | 20.0% |
| !! | TATAGATTGATGATAGATGA+AGG | - | chr2.4:3020612-3020631 | None:intergenic | 25.0% |
| !!! | GTTGTGGTTTTTTTTAATAC+AGG | + | chr2.4:3021096-3021115 | MS.gene85110:intron | 25.0% |
| CCACAACATATCAATGATTA+AGG | - | chr2.4:3021083-3021102 | None:intergenic | 30.0% | |
| CCTTAATCATTGATATGTTG+TGG | + | chr2.4:3021080-3021099 | MS.gene85110:intron | 30.0% | |
| GTTTATGTGAATGAATTGCA+GGG | + | chr2.4:3020668-3020687 | MS.gene85110:intron | 30.0% | |
| TTTAGATAACTGGAGTGAAA+AGG | + | chr2.4:3020913-3020932 | MS.gene85110:intron | 30.0% | |
| CGTTTATGTGAATGAATTGC+AGG | + | chr2.4:3020667-3020686 | MS.gene85110:intron | 35.0% | |
| CTATACGATTTCGTATATGC+TGG | + | chr2.4:3020627-3020646 | MS.gene85110:intron | 35.0% | |
| ! | AGATTGATGATAGATGAAGG+AGG | - | chr2.4:3020609-3020628 | None:intergenic | 35.0% |
| CAGTTCCAGTAGTCTTTCTA+CGG | - | chr2.4:3020943-3020962 | None:intergenic | 40.0% | |
| GTGAATGAATTGCAGGGTAA+GGG | + | chr2.4:3020674-3020693 | MS.gene85110:intron | 40.0% | |
| TGATGATAGATGAAGGAGGA+AGG | - | chr2.4:3020605-3020624 | None:intergenic | 40.0% | |
| TGTGAATGAATTGCAGGGTA+AGG | + | chr2.4:3020673-3020692 | MS.gene85110:intron | 40.0% | |
| !! | CTCTAAAACCGCTCTTGAAT+CGG | - | chr2.4:3020997-3021016 | None:intergenic | 40.0% |
| !!! | ATTCAAGAGCGGTTTTAGAG+AGG | + | chr2.4:3020997-3021016 | MS.gene85110:CDS | 40.0% |
| !!! | TTCAAGAGCGGTTTTAGAGA+GGG | + | chr2.4:3020998-3021017 | MS.gene85110:CDS | 40.0% |
| ATTTCCTAACACGAGCAGCT+GGG | - | chr2.4:3020805-3020824 | None:intergenic | 45.0% | |
| CGTAGAAAGACTACTGGAAC+TGG | + | chr2.4:3020941-3020960 | MS.gene85110:CDS | 45.0% | |
| CTGGAAGTTTCGGTAAGAGA+AGG | + | chr2.4:3020699-3020718 | MS.gene85110:CDS | 45.0% | |
| GAAAGACTACTGGAACTGGT+AGG | + | chr2.4:3020945-3020964 | MS.gene85110:CDS | 45.0% | |
| GCAATCCGTAGAAAGACTAC+TGG | + | chr2.4:3020935-3020954 | MS.gene85110:CDS | 45.0% | |
| GGTAAGGGAACTGGAAGTTT+CGG | + | chr2.4:3020689-3020708 | MS.gene85110:CDS | 45.0% | |
| ! | AAGCAGAGCATCTACTCTTC+TGG | - | chr2.4:3020775-3020794 | None:intergenic | 45.0% |
| ! | AGCATCTACTCTTCTGGAGA+TGG | - | chr2.4:3020769-3020788 | None:intergenic | 45.0% |
| ! | TCTTCTGGAGATGGAAACTG+CGG | - | chr2.4:3020760-3020779 | None:intergenic | 45.0% |
| AGACTCACACACTCTGTGTC+AGG | + | chr2.4:3020726-3020745 | MS.gene85110:CDS | 50.0% | |
| CATTTCCTAACACGAGCAGC+TGG | - | chr2.4:3020806-3020825 | None:intergenic | 50.0% | |
| CTACTGGAACTGGTAGGATG+AGG | + | chr2.4:3020951-3020970 | MS.gene85110:CDS | 50.0% | |
| GAAGCTGCACCTAGGAAGAA+GGG | + | chr2.4:3021123-3021142 | MS.gene85110:CDS | 50.0% | |
| GAATTGCAGGGTAAGGGAAC+TGG | + | chr2.4:3020680-3020699 | MS.gene85110:intron | 50.0% | |
| TGAAGCTGCACCTAGGAAGA+AGG | + | chr2.4:3021122-3021141 | MS.gene85110:CDS | 50.0% | |
| AAACCGCTCTTGAATCGGCG+AGG | - | chr2.4:3020992-3021011 | None:intergenic | 55.0% | |
| CACACACTCTGTGTCAGGTG+TGG | + | chr2.4:3020731-3020750 | MS.gene85110:CDS | 55.0% | |
| CAGGAACTGAAGCTGCACCT+AGG | + | chr2.4:3021115-3021134 | MS.gene85110:intron | 55.0% | |
| CTTACCCAGCTGCTCGTGTT+AGG | + | chr2.4:3020798-3020817 | MS.gene85110:CDS | 55.0% | |
| TCTGGAGATGGAAACTGCGG+CGG | - | chr2.4:3020757-3020776 | None:intergenic | 55.0% | |
| !! | GTTCCTCGCCGATTCAAGAG+CGG | + | chr2.4:3020986-3021005 | MS.gene85110:CDS | 55.0% |
| GACGCAGCTCCCTTCTTCCT+AGG | - | chr2.4:3021135-3021154 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 3020590 | 3021161 | 3020590 | ID=MS.gene85110 |
| chr2.4 | mRNA | 3020590 | 3021161 | 3020590 | ID=MS.gene85110.t1;Parent=MS.gene85110 |
| chr2.4 | exon | 3020590 | 3020592 | 3020590 | ID=MS.gene85110.t1.exon1;Parent=MS.gene85110.t1 |
| chr2.4 | CDS | 3020590 | 3020592 | 3020590 | ID=cds.MS.gene85110.t1;Parent=MS.gene85110.t1 |
| chr2.4 | exon | 3020689 | 3020824 | 3020689 | ID=MS.gene85110.t1.exon2;Parent=MS.gene85110.t1 |
| chr2.4 | CDS | 3020689 | 3020824 | 3020689 | ID=cds.MS.gene85110.t1;Parent=MS.gene85110.t1 |
| chr2.4 | exon | 3020918 | 3021019 | 3020918 | ID=MS.gene85110.t1.exon3;Parent=MS.gene85110.t1 |
| chr2.4 | CDS | 3020918 | 3021019 | 3020918 | ID=cds.MS.gene85110.t1;Parent=MS.gene85110.t1 |
| chr2.4 | exon | 3021118 | 3021161 | 3021118 | ID=MS.gene85110.t1.exon4;Parent=MS.gene85110.t1 |
| chr2.4 | CDS | 3021118 | 3021161 | 3021118 | ID=cds.MS.gene85110.t1;Parent=MS.gene85110.t1 |
| Gene Sequence |
| Protein sequence |
