Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47962.t1 | XP_013446712.1 | 98.5 | 65 | 1 | 0 | 1 | 65 | 79 | 143 | 1.00E-26 | 128.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47962.t1 | A0A072TT93 | 98.5 | 65 | 1 | 0 | 1 | 65 | 79 | 143 | 7.4e-27 | 128.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049501 | MS.gene47962 | 0.815351 | 9.67E-52 | -1.69E-46 |
| MS.gene050355 | MS.gene47962 | 0.815608 | 8.47E-52 | -1.69E-46 |
| MS.gene051247 | MS.gene47962 | 0.810221 | 1.28E-50 | -1.69E-46 |
| MS.gene052582 | MS.gene47962 | 0.820383 | 7.06E-53 | -1.69E-46 |
| MS.gene052584 | MS.gene47962 | 0.828217 | 1.02E-54 | -1.69E-46 |
| MS.gene052867 | MS.gene47962 | 0.809721 | 1.64E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene47962 | 0.811413 | 7.09E-51 | -1.69E-46 |
| MS.gene054291 | MS.gene47962 | -0.809407 | 1.92E-50 | -1.69E-46 |
| MS.gene05468 | MS.gene47962 | 0.803565 | 3.28E-49 | -1.69E-46 |
| MS.gene055166 | MS.gene47962 | 0.800871 | 1.18E-48 | -1.69E-46 |
| MS.gene055357 | MS.gene47962 | 0.81066 | 1.03E-50 | -1.69E-46 |
| MS.gene055808 | MS.gene47962 | 0.803182 | 3.94E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene47962 | 0.80948 | 1.85E-50 | -1.69E-46 |
| MS.gene05659 | MS.gene47962 | 0.800195 | 1.61E-48 | -1.69E-46 |
| MS.gene057314 | MS.gene47962 | 0.81372 | 2.22E-51 | -1.69E-46 |
| MS.gene058037 | MS.gene47962 | 0.8039 | 2.80E-49 | -1.69E-46 |
| MS.gene058084 | MS.gene47962 | 0.802251 | 6.13E-49 | -1.69E-46 |
| MS.gene058908 | MS.gene47962 | 0.85512 | 7.81E-62 | -1.69E-46 |
| MS.gene058911 | MS.gene47962 | 0.83696 | 6.89E-57 | -1.69E-46 |
| MS.gene05954 | MS.gene47962 | 0.819045 | 1.43E-52 | -1.69E-46 |
| MS.gene060137 | MS.gene47962 | -0.811722 | 6.07E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene47962 | 0.810385 | 1.18E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene47962 | -0.80072 | 1.26E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47962.t1 | MTR_8g089655 | 98.462 | 65 | 1 | 0 | 1 | 65 | 79 | 143 | 1.56e-42 | 134 |
| MS.gene47962.t1 | MTR_8g089655 | 98.462 | 65 | 1 | 0 | 1 | 65 | 82 | 146 | 1.56e-42 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47962.t1 | AT1G16790 | 60.714 | 56 | 22 | 0 | 1 | 56 | 79 | 134 | 3.62e-21 | 80.5 |
Find 17 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGCAATTGGGGTTGTTTC+TGG | 0.158557 | 4.1:-11011661 | MS.gene47962:CDS |
| GTTTCTGGTGCTGCTCTTAA+TGG | 0.209111 | 4.1:-11011646 | MS.gene47962:CDS |
| TTTCACCTATTGCTCAGATT+TGG | 0.309135 | 4.1:-11011597 | MS.gene47962:CDS |
| GGTGCTGCTCTTAATGGAAT+TGG | 0.362930 | 4.1:-11011640 | MS.gene47962:CDS |
| TTGTTGAGACGTTTGCAATT+GGG | 0.377010 | 4.1:-11011672 | MS.gene47962:CDS |
| TTTGTTGAGACGTTTGCAAT+TGG | 0.400517 | 4.1:-11011673 | MS.gene47962:CDS |
| GAATTCCAAATCTGAGCAAT+AGG | 0.408372 | 4.1:+11011592 | None:intergenic |
| TCATTGAGGGGTAGATTTAG+TGG | 0.436309 | 4.1:+11011558 | None:intergenic |
| ATGAAGCTTGATACTAAGCC+TGG | 0.457946 | 4.1:-11011697 | MS.gene47962:CDS |
| TGTTGAGACGTTTGCAATTG+GGG | 0.507433 | 4.1:-11011671 | MS.gene47962:CDS |
| ATCTTCTAGAAAGATGGATT+CGG | 0.522036 | 4.1:-11011746 | None:intergenic |
| AGGGGTAGATTTAGTGGCTG+AGG | 0.534389 | 4.1:+11011564 | None:intergenic |
| AGGGTGCAAAATGTCATTGA+GGG | 0.536281 | 4.1:+11011545 | None:intergenic |
| AAGGGTGCAAAATGTCATTG+AGG | 0.553529 | 4.1:+11011544 | None:intergenic |
| GCAAACGTCTCAACAAATCC+AGG | 0.561055 | 4.1:+11011679 | None:intergenic |
| GCAATAGGTGAAAGAATGTG+AGG | 0.689368 | 4.1:+11011607 | None:intergenic |
| GGGTGCAAAATGTCATTGAG+GGG | 0.694593 | 4.1:+11011546 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAATTCCAAATCTGAGCAAT+AGG | + | chr4.1:11011702-11011721 | None:intergenic | 35.0% | |
| TTGTTGAGACGTTTGCAATT+GGG | - | chr4.1:11011619-11011638 | MS.gene47962:CDS | 35.0% | |
| TTTCACCTATTGCTCAGATT+TGG | - | chr4.1:11011694-11011713 | MS.gene47962:CDS | 35.0% | |
| TTTGTTGAGACGTTTGCAAT+TGG | - | chr4.1:11011618-11011637 | MS.gene47962:CDS | 35.0% | |
| ATGAAGCTTGATACTAAGCC+TGG | - | chr4.1:11011594-11011613 | MS.gene47962:CDS | 40.0% | |
| GCAATAGGTGAAAGAATGTG+AGG | + | chr4.1:11011687-11011706 | None:intergenic | 40.0% | |
| TCATTGAGGGGTAGATTTAG+TGG | + | chr4.1:11011736-11011755 | None:intergenic | 40.0% | |
| TGTTGAGACGTTTGCAATTG+GGG | - | chr4.1:11011620-11011639 | MS.gene47962:CDS | 40.0% | |
| ! | TTTGCAATTGGGGTTGTTTC+TGG | - | chr4.1:11011630-11011649 | MS.gene47962:CDS | 40.0% |
| GCAAACGTCTCAACAAATCC+AGG | + | chr4.1:11011615-11011634 | None:intergenic | 45.0% | |
| !! | GGTGCTGCTCTTAATGGAAT+TGG | - | chr4.1:11011651-11011670 | MS.gene47962:CDS | 45.0% |
| !! | GTTTCTGGTGCTGCTCTTAA+TGG | - | chr4.1:11011645-11011664 | MS.gene47962:CDS | 45.0% |
| AGGGGTAGATTTAGTGGCTG+AGG | + | chr4.1:11011730-11011749 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 11011558 | 11011755 | 11011558 | ID=MS.gene47962 |
| chr4.1 | mRNA | 11011558 | 11011755 | 11011558 | ID=MS.gene47962.t1;Parent=MS.gene47962 |
| chr4.1 | exon | 11011558 | 11011755 | 11011558 | ID=MS.gene47962.t1.exon1;Parent=MS.gene47962.t1 |
| chr4.1 | CDS | 11011558 | 11011755 | 11011558 | ID=cds.MS.gene47962.t1;Parent=MS.gene47962.t1 |
| Gene Sequence |
| Protein sequence |
