Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48314.t1 | XP_013457732.1 | 95.6 | 206 | 9 | 0 | 1 | 206 | 1 | 206 | 1.70E-107 | 398.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48314.t1 | Q9FGS5 | 51.2 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.3e-47 | 188.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48314.t1 | I3SMV0 | 95.6 | 206 | 9 | 0 | 1 | 206 | 1 | 206 | 1.2e-107 | 398.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049634 | MS.gene48314 | 0.827481 | 1.53E-54 | -1.69E-46 |
| MS.gene050095 | MS.gene48314 | 0.81385 | 2.08E-51 | -1.69E-46 |
| MS.gene050096 | MS.gene48314 | 0.850447 | 1.69E-60 | -1.69E-46 |
| MS.gene050380 | MS.gene48314 | 0.80646 | 8.14E-50 | -1.69E-46 |
| MS.gene050381 | MS.gene48314 | 0.82281 | 1.94E-53 | -1.69E-46 |
| MS.gene050809 | MS.gene48314 | 0.811592 | 6.48E-51 | -1.69E-46 |
| MS.gene05104 | MS.gene48314 | 0.801718 | 7.89E-49 | -1.69E-46 |
| MS.gene053027 | MS.gene48314 | 0.807871 | 4.09E-50 | -1.69E-46 |
| MS.gene053031 | MS.gene48314 | 0.826764 | 2.27E-54 | -1.69E-46 |
| MS.gene053033 | MS.gene48314 | 0.801079 | 1.07E-48 | -1.69E-46 |
| MS.gene053406 | MS.gene48314 | 0.814094 | 1.84E-51 | -1.69E-46 |
| MS.gene054290 | MS.gene48314 | 0.82883 | 7.23E-55 | -1.69E-46 |
| MS.gene055124 | MS.gene48314 | 0.843026 | 1.80E-58 | -1.69E-46 |
| MS.gene055959 | MS.gene48314 | 0.83173 | 1.41E-55 | -1.69E-46 |
| MS.gene058093 | MS.gene48314 | 0.822014 | 2.97E-53 | -1.69E-46 |
| MS.gene058099 | MS.gene48314 | 0.801526 | 8.64E-49 | -1.69E-46 |
| MS.gene058179 | MS.gene48314 | 0.835521 | 1.60E-56 | -1.69E-46 |
| MS.gene058840 | MS.gene48314 | 0.846299 | 2.37E-59 | -1.69E-46 |
| MS.gene059037 | MS.gene48314 | 0.828604 | 8.20E-55 | -1.69E-46 |
| MS.gene059038 | MS.gene48314 | 0.818003 | 2.46E-52 | -1.69E-46 |
| MS.gene059045 | MS.gene48314 | 0.806745 | 7.09E-50 | -1.69E-46 |
| MS.gene059944 | MS.gene48314 | 0.844599 | 6.84E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48314.t1 | MTR_4g104730 | 95.146 | 206 | 10 | 0 | 1 | 206 | 1 | 206 | 1.07e-149 | 414 |
| MS.gene48314.t1 | MTR_4g104700 | 95.631 | 206 | 9 | 0 | 1 | 206 | 1 | 206 | 3.57e-141 | 392 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48314.t1 | AT5G50200 | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.32e-65 | 199 |
| MS.gene48314.t1 | AT5G50200 | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.32e-65 | 199 |
| MS.gene48314.t1 | AT5G50200 | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.32e-65 | 199 |
| MS.gene48314.t1 | AT4G24715 | 49.275 | 207 | 97 | 5 | 1 | 202 | 23 | 226 | 6.97e-63 | 195 |
Find 54 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAGCATAACTTCAGCTTTA+TGG | 0.105787 | 8.3:-17796525 | None:intergenic |
| CATTGTCTTCAGTGCTTTCT+CGG | 0.283646 | 8.3:+17796847 | MS.gene48314:CDS |
| ATCTATGCAGTTATGTTATC+TGG | 0.287035 | 8.3:+17796431 | MS.gene48314:intron |
| CTCAAATTCAATCACTGCTT+TGG | 0.355774 | 8.3:-17796920 | None:intergenic |
| CGCATTTGATTCCAATGATA+AGG | 0.357587 | 8.3:+17796727 | MS.gene48314:CDS |
| TACTTACCTTGTCCATGCTT+AGG | 0.361592 | 8.3:-17796309 | None:intergenic |
| ACTTACCTTGTCCATGCTTA+GGG | 0.362581 | 8.3:-17796308 | None:intergenic |
| TTTGAGATCAATGCAATTAG+TGG | 0.365486 | 8.3:+17796797 | MS.gene48314:CDS |
| TCTTACCCGTGTTCGTGGTT+TGG | 0.369045 | 8.3:-17796763 | None:intergenic |
| ATAAGCCACTTCCTTATCAT+TGG | 0.373409 | 8.3:-17796738 | None:intergenic |
| GAATCAACCACTCGAAGGTT+TGG | 0.410270 | 8.3:-17796664 | None:intergenic |
| TGTTTAGCTGAAATTTGTTA+TGG | 0.421382 | 8.3:+17796237 | MS.gene48314:CDS |
| AATGATAAGGAAGTGGCTTA+TGG | 0.425454 | 8.3:+17796740 | MS.gene48314:CDS |
| GATTCTGCGCGACGTGCCTC+AGG | 0.444465 | 8.3:+17796682 | MS.gene48314:CDS |
| GCTTTCTCGGTTGTGTCCCT+AGG | 0.446758 | 8.3:+17796860 | MS.gene48314:CDS |
| GTCCCTGGAGAGTTCATCCT+CGG | 0.457081 | 8.3:-17796585 | None:intergenic |
| TAAGAAGAGTCTGTTCCAGC+TGG | 0.469266 | 8.3:-17796497 | None:intergenic |
| GCTACTCTTACCCGTGTTCG+TGG | 0.490262 | 8.3:-17796768 | None:intergenic |
| AGCTGAAATTTGTTATGGAA+AGG | 0.496497 | 8.3:+17796242 | MS.gene48314:CDS |
| CTTCTATATTGAAAAGAGGA+AGG | 0.500127 | 8.3:+17796889 | MS.gene48314:CDS |
| AGACCGAGGATGAACTCTCC+AGG | 0.511838 | 8.3:+17796582 | MS.gene48314:CDS |
| TGATTCCAATGATAAGGAAG+TGG | 0.518875 | 8.3:+17796733 | MS.gene48314:CDS |
| TGTTCTTCTATATTGAAAAG+AGG | 0.526365 | 8.3:+17796885 | MS.gene48314:CDS |
| GTTGGAAGCATTGTAGGGCA+TGG | 0.526614 | 8.3:-17796633 | None:intergenic |
| TAGGGGAAGCTGTGACCTCA+AGG | 0.526901 | 8.3:-17796290 | None:intergenic |
| CAATATAGAAGAACACGCCT+AGG | 0.537687 | 8.3:-17796877 | None:intergenic |
| CTACAATGCTTCCAACAAAA+CGG | 0.547064 | 8.3:+17796640 | MS.gene48314:CDS |
| TAGACAAAATCAGTGGAACA+TGG | 0.559795 | 8.3:+17796456 | MS.gene48314:CDS |
| TTCTATATTGAAAAGAGGAA+GGG | 0.562873 | 8.3:+17796890 | MS.gene48314:CDS |
| CGAGAAAGCACTGAAGACAA+TGG | 0.583492 | 8.3:-17796846 | None:intergenic |
| GTCACAGCTTCCCCTAAGCA+TGG | 0.584742 | 8.3:+17796297 | MS.gene48314:CDS |
| CTAAACAAGACTTTCCCAGC+TGG | 0.586305 | 8.3:+17796482 | MS.gene48314:CDS |
| TATGGCCAAACCACGAACAC+GGG | 0.596066 | 8.3:+17796758 | MS.gene48314:CDS |
| TTATGGCCAAACCACGAACA+CGG | 0.597854 | 8.3:+17796757 | MS.gene48314:CDS |
| CTTACCTTGTCCATGCTTAG+GGG | 0.615805 | 8.3:-17796307 | None:intergenic |
| AGACAAAATCAGTGGAACAT+GGG | 0.618150 | 8.3:+17796457 | MS.gene48314:CDS |
| TATGGCAGCACACAAGCTTG+TGG | 0.620979 | 8.3:+17796194 | None:intergenic |
| TTCTTTGAAAAGAACCCTTG+AGG | 0.622622 | 8.3:+17796275 | MS.gene48314:CDS |
| AAGAAGAGTCTGTTCCAGCT+GGG | 0.623941 | 8.3:-17796496 | None:intergenic |
| GACCGAGGATGAACTCTCCA+GGG | 0.630791 | 8.3:+17796583 | MS.gene48314:CDS |
| AGGGGAAGCTGTGACCTCAA+GGG | 0.635053 | 8.3:-17796289 | None:intergenic |
| AATATAGAAGAACACGCCTA+GGG | 0.636139 | 8.3:-17796876 | None:intergenic |
| ATCATTGGAATCAAATGCGT+AGG | 0.647510 | 8.3:-17796723 | None:intergenic |
| CCTCGGTCTTTCTCCAAGCG+CGG | 0.651070 | 8.3:-17796568 | None:intergenic |
| GCGCAGAATCAACCACTCGA+AGG | 0.651463 | 8.3:-17796669 | None:intergenic |
| TCTGGAGTAGACAAAATCAG+TGG | 0.660740 | 8.3:+17796449 | MS.gene48314:CDS |
| GCTTCCCCTAAGCATGGACA+AGG | 0.668529 | 8.3:+17796303 | MS.gene48314:CDS |
| CGTACAAAGTACGTGGCCTG+AGG | 0.676173 | 8.3:-17796698 | None:intergenic |
| AAACGGTCCAAACCTTCGAG+TGG | 0.693663 | 8.3:+17796657 | MS.gene48314:CDS |
| AGCAGATATCAAGAGTCGCG+TGG | 0.697710 | 8.3:-17796823 | None:intergenic |
| AGATATCAAGAGTCGCGTGG+CGG | 0.700580 | 8.3:-17796820 | None:intergenic |
| CCGCGCTTGGAGAAAGACCG+AGG | 0.703551 | 8.3:+17796568 | MS.gene48314:CDS |
| AAAGCTGAAGTTATGCTACG+CGG | 0.775950 | 8.3:+17796529 | MS.gene48314:CDS |
| GTAGGCACGTACAAAGTACG+TGG | 0.784284 | 8.3:-17796705 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAATTGAGTTTATATTGA+AGG | - | chr8.3:17796402-17796421 | None:intergenic | 20.0% |
| ! | TAATTAAATACGCCAATACA+AGG | - | chr8.3:17796351-17796370 | None:intergenic | 25.0% |
| ! | TGTTCTTCTATATTGAAAAG+AGG | + | chr8.3:17796885-17796904 | MS.gene48314:CDS | 25.0% |
| ! | TTCTATATTGAAAAGAGGAA+GGG | + | chr8.3:17796890-17796909 | MS.gene48314:CDS | 25.0% |
| !!! | TGTTTAGCTGAAATTTGTTA+TGG | + | chr8.3:17796237-17796256 | MS.gene48314:CDS | 25.0% |
| ATCTATGCAGTTATGTTATC+TGG | + | chr8.3:17796431-17796450 | MS.gene48314:intron | 30.0% | |
| CTTCTATATTGAAAAGAGGA+AGG | + | chr8.3:17796889-17796908 | MS.gene48314:CDS | 30.0% | |
| ! | TAAGATTTTGCACCTTGTAT+TGG | + | chr8.3:17796336-17796355 | MS.gene48314:intron | 30.0% |
| ! | TTTGAGATCAATGCAATTAG+TGG | + | chr8.3:17796797-17796816 | MS.gene48314:CDS | 30.0% |
| !! | AGCTGAAATTTGTTATGGAA+AGG | + | chr8.3:17796242-17796261 | MS.gene48314:CDS | 30.0% |
| AATATAGAAGAACACGCCTA+GGG | - | chr8.3:17796879-17796898 | None:intergenic | 35.0% | |
| AATGATAAGGAAGTGGCTTA+TGG | + | chr8.3:17796740-17796759 | MS.gene48314:CDS | 35.0% | |
| AGACAAAATCAGTGGAACAT+GGG | + | chr8.3:17796457-17796476 | MS.gene48314:CDS | 35.0% | |
| ATAAGCCACTTCCTTATCAT+TGG | - | chr8.3:17796741-17796760 | None:intergenic | 35.0% | |
| ATCATTGGAATCAAATGCGT+AGG | - | chr8.3:17796726-17796745 | None:intergenic | 35.0% | |
| CGCATTTGATTCCAATGATA+AGG | + | chr8.3:17796727-17796746 | MS.gene48314:CDS | 35.0% | |
| CTACAATGCTTCCAACAAAA+CGG | + | chr8.3:17796640-17796659 | MS.gene48314:CDS | 35.0% | |
| GTAGCATAACTTCAGCTTTA+TGG | - | chr8.3:17796528-17796547 | None:intergenic | 35.0% | |
| TAGACAAAATCAGTGGAACA+TGG | + | chr8.3:17796456-17796475 | MS.gene48314:CDS | 35.0% | |
| TGATTCCAATGATAAGGAAG+TGG | + | chr8.3:17796733-17796752 | MS.gene48314:CDS | 35.0% | |
| ! | TTCTTTGAAAAGAACCCTTG+AGG | + | chr8.3:17796275-17796294 | MS.gene48314:CDS | 35.0% |
| !!! | GTTTTGTTGGAAGCATTGTA+GGG | - | chr8.3:17796641-17796660 | None:intergenic | 35.0% |
| AAAGCTGAAGTTATGCTACG+CGG | + | chr8.3:17796529-17796548 | MS.gene48314:CDS | 40.0% | |
| ACTTACCTTGTCCATGCTTA+GGG | - | chr8.3:17796311-17796330 | None:intergenic | 40.0% | |
| CAATATAGAAGAACACGCCT+AGG | - | chr8.3:17796880-17796899 | None:intergenic | 40.0% | |
| CATTGTCTTCAGTGCTTTCT+CGG | + | chr8.3:17796847-17796866 | MS.gene48314:CDS | 40.0% | |
| TACTTACCTTGTCCATGCTT+AGG | - | chr8.3:17796312-17796331 | None:intergenic | 40.0% | |
| ! | TCTGGAGTAGACAAAATCAG+TGG | + | chr8.3:17796449-17796468 | MS.gene48314:CDS | 40.0% |
| !!! | CGTTTTGTTGGAAGCATTGT+AGG | - | chr8.3:17796642-17796661 | None:intergenic | 40.0% |
| AAGAAGAGTCTGTTCCAGCT+GGG | - | chr8.3:17796499-17796518 | None:intergenic | 45.0% | |
| CTAAACAAGACTTTCCCAGC+TGG | + | chr8.3:17796482-17796501 | MS.gene48314:CDS | 45.0% | |
| CTTACCTTGTCCATGCTTAG+GGG | - | chr8.3:17796310-17796329 | None:intergenic | 45.0% | |
| GAATCAACCACTCGAAGGTT+TGG | - | chr8.3:17796667-17796686 | None:intergenic | 45.0% | |
| TAAGAAGAGTCTGTTCCAGC+TGG | - | chr8.3:17796500-17796519 | None:intergenic | 45.0% | |
| TTATGGCCAAACCACGAACA+CGG | + | chr8.3:17796757-17796776 | MS.gene48314:CDS | 45.0% | |
| ! | GAAGGTTTGGACCGTTTTGT+TGG | - | chr8.3:17796654-17796673 | None:intergenic | 45.0% |
| !! | CGAGAAAGCACTGAAGACAA+TGG | - | chr8.3:17796849-17796868 | None:intergenic | 45.0% |
| !! | GTGTTGACAAGTTTTGTCCC+TGG | - | chr8.3:17796603-17796622 | None:intergenic | 45.0% |
| AAACGGTCCAAACCTTCGAG+TGG | + | chr8.3:17796657-17796676 | MS.gene48314:CDS | 50.0% | |
| AGATATCAAGAGTCGCGTGG+CGG | - | chr8.3:17796823-17796842 | None:intergenic | 50.0% | |
| AGCAGATATCAAGAGTCGCG+TGG | - | chr8.3:17796826-17796845 | None:intergenic | 50.0% | |
| GTAGGCACGTACAAAGTACG+TGG | - | chr8.3:17796708-17796727 | None:intergenic | 50.0% | |
| TAAGCCAAAAAGACCGCGCT+TGG | + | chr8.3:17796555-17796574 | MS.gene48314:CDS | 50.0% | |
| TATGGCCAAACCACGAACAC+GGG | + | chr8.3:17796758-17796777 | MS.gene48314:CDS | 50.0% | |
| TCTTACCCGTGTTCGTGGTT+TGG | - | chr8.3:17796766-17796785 | None:intergenic | 50.0% | |
| ! | TTCTCCAAGCGCGGTCTTTT+TGG | - | chr8.3:17796562-17796581 | None:intergenic | 50.0% |
| !! | GTTGGAAGCATTGTAGGGCA+TGG | - | chr8.3:17796636-17796655 | None:intergenic | 50.0% |
| !!! | AGCGCGGTCTTTTTGGCTTA+TGG | - | chr8.3:17796555-17796574 | None:intergenic | 50.0% |
| AGACCGAGGATGAACTCTCC+AGG | + | chr8.3:17796582-17796601 | MS.gene48314:CDS | 55.0% | |
| AGGGGAAGCTGTGACCTCAA+GGG | - | chr8.3:17796292-17796311 | None:intergenic | 55.0% | |
| CGTACAAAGTACGTGGCCTG+AGG | - | chr8.3:17796701-17796720 | None:intergenic | 55.0% | |
| GACCGAGGATGAACTCTCCA+GGG | + | chr8.3:17796583-17796602 | MS.gene48314:CDS | 55.0% | |
| GCGCAGAATCAACCACTCGA+AGG | - | chr8.3:17796672-17796691 | None:intergenic | 55.0% | |
| GCTACTCTTACCCGTGTTCG+TGG | - | chr8.3:17796771-17796790 | None:intergenic | 55.0% | |
| GCTTCCCCTAAGCATGGACA+AGG | + | chr8.3:17796303-17796322 | MS.gene48314:CDS | 55.0% | |
| GCTTTCTCGGTTGTGTCCCT+AGG | + | chr8.3:17796860-17796879 | MS.gene48314:CDS | 55.0% | |
| GTCACAGCTTCCCCTAAGCA+TGG | + | chr8.3:17796297-17796316 | MS.gene48314:CDS | 55.0% | |
| GTCCCTGGAGAGTTCATCCT+CGG | - | chr8.3:17796588-17796607 | None:intergenic | 55.0% | |
| TAGGGGAAGCTGTGACCTCA+AGG | - | chr8.3:17796293-17796312 | None:intergenic | 55.0% | |
| CCTCGGTCTTTCTCCAAGCG+CGG | - | chr8.3:17796571-17796590 | None:intergenic | 60.0% | |
| CCGCGCTTGGAGAAAGACCG+AGG | + | chr8.3:17796568-17796587 | MS.gene48314:CDS | 65.0% | |
| GATTCTGCGCGACGTGCCTC+AGG | + | chr8.3:17796682-17796701 | MS.gene48314:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 17796195 | 17796931 | 17796195 | ID=MS.gene48314 |
| chr8.3 | mRNA | 17796195 | 17796931 | 17796195 | ID=MS.gene48314.t1;Parent=MS.gene48314 |
| chr8.3 | exon | 17796195 | 17796324 | 17796195 | ID=MS.gene48314.t1.exon1;Parent=MS.gene48314.t1 |
| chr8.3 | CDS | 17796195 | 17796324 | 17796195 | ID=cds.MS.gene48314.t1;Parent=MS.gene48314.t1 |
| chr8.3 | exon | 17796441 | 17796931 | 17796441 | ID=MS.gene48314.t1.exon2;Parent=MS.gene48314.t1 |
| chr8.3 | CDS | 17796441 | 17796931 | 17796441 | ID=cds.MS.gene48314.t1;Parent=MS.gene48314.t1 |
| Gene Sequence |
| Protein sequence |
