Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48756.t1 | XP_003605375.1 | 97.8 | 922 | 18 | 1 | 1 | 920 | 1 | 922 | 0.00E+00 | 1567.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48756.t1 | Q9LQ55 | 76.5 | 873 | 187 | 7 | 1 | 864 | 1 | 864 | 0.0e+00 | 1229.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48756.t1 | G7JKQ6 | 97.8 | 922 | 18 | 1 | 1 | 920 | 1 | 922 | 0.0e+00 | 1567.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049193 | MS.gene48756 | 0.834208 | 3.42E-56 | -1.69E-46 |
| MS.gene050213 | MS.gene48756 | 0.813138 | 2.98E-51 | -1.69E-46 |
| MS.gene050257 | MS.gene48756 | 0.832439 | 9.45E-56 | -1.69E-46 |
| MS.gene050258 | MS.gene48756 | 0.833852 | 4.20E-56 | -1.69E-46 |
| MS.gene050646 | MS.gene48756 | 0.801881 | 7.30E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene48756 | 0.814779 | 1.29E-51 | -1.69E-46 |
| MS.gene051234 | MS.gene48756 | 0.827642 | 1.40E-54 | -1.69E-46 |
| MS.gene051960 | MS.gene48756 | 0.805321 | 1.41E-49 | -1.69E-46 |
| MS.gene052307 | MS.gene48756 | 0.818971 | 1.48E-52 | -1.69E-46 |
| MS.gene052498 | MS.gene48756 | 0.803313 | 3.70E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene48756 | 0.827192 | 1.79E-54 | -1.69E-46 |
| MS.gene053143 | MS.gene48756 | 0.855235 | 7.23E-62 | -1.69E-46 |
| MS.gene054004 | MS.gene48756 | 0.809085 | 2.25E-50 | -1.69E-46 |
| MS.gene054585 | MS.gene48756 | 0.85162 | 7.88E-61 | -1.69E-46 |
| MS.gene055886 | MS.gene48756 | 0.822352 | 2.48E-53 | -1.69E-46 |
| MS.gene057148 | MS.gene48756 | -0.812139 | 4.93E-51 | -1.69E-46 |
| MS.gene057174 | MS.gene48756 | 0.858277 | 9.20E-63 | -1.69E-46 |
| MS.gene058095 | MS.gene48756 | 0.841769 | 3.89E-58 | -1.69E-46 |
| MS.gene059778 | MS.gene48756 | 0.861311 | 1.12E-63 | -1.69E-46 |
| MS.gene060522 | MS.gene48756 | 0.865962 | 4.04E-65 | -1.69E-46 |
| MS.gene060933 | MS.gene48756 | 0.80841 | 3.14E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene48756 | 0.823556 | 1.30E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48756.t1 | MTR_4g030140 | 98.265 | 922 | 14 | 1 | 1 | 920 | 1 | 922 | 0.0 | 1844 |
| MS.gene48756.t1 | MTR_4g134680 | 76.452 | 930 | 192 | 11 | 1 | 920 | 1 | 913 | 0.0 | 1381 |
| MS.gene48756.t1 | MTR_4g134680 | 76.832 | 846 | 175 | 7 | 1 | 840 | 1 | 831 | 0.0 | 1288 |
| MS.gene48756.t1 | MTR_4g134680 | 75.616 | 771 | 167 | 7 | 1 | 765 | 1 | 756 | 0.0 | 1153 |
| MS.gene48756.t1 | MTR_4g134680 | 78.378 | 629 | 114 | 9 | 301 | 920 | 1 | 616 | 0.0 | 949 |
| MS.gene48756.t1 | MTR_4g022340 | 75.159 | 314 | 49 | 3 | 1 | 308 | 1 | 291 | 8.05e-148 | 439 |
| MS.gene48756.t1 | MTR_1g032550 | 32.749 | 513 | 310 | 10 | 1 | 498 | 1 | 493 | 7.55e-68 | 238 |
| MS.gene48756.t1 | MTR_8g105580 | 31.445 | 512 | 317 | 9 | 1 | 498 | 1 | 492 | 5.67e-66 | 232 |
| MS.gene48756.t1 | MTR_8g105580 | 31.445 | 512 | 317 | 9 | 1 | 498 | 1 | 492 | 8.54e-66 | 233 |
| MS.gene48756.t1 | MTR_4g098920 | 29.944 | 531 | 317 | 11 | 4 | 508 | 1 | 502 | 1.30e-61 | 221 |
| MS.gene48756.t1 | MTR_8g009510 | 30.502 | 518 | 330 | 7 | 1 | 503 | 1 | 503 | 1.35e-61 | 221 |
| MS.gene48756.t1 | MTR_7g451010 | 30.502 | 518 | 330 | 7 | 1 | 503 | 1 | 503 | 5.79e-59 | 213 |
| MS.gene48756.t1 | MTR_8g027675 | 29.264 | 516 | 329 | 11 | 4 | 503 | 1 | 496 | 1.08e-57 | 209 |
| MS.gene48756.t1 | MTR_8g099550 | 29.885 | 522 | 319 | 12 | 4 | 503 | 1 | 497 | 1.08e-57 | 209 |
| MS.gene48756.t1 | MTR_5g009150 | 29.094 | 519 | 328 | 11 | 4 | 503 | 1 | 498 | 3.84e-55 | 202 |
| MS.gene48756.t1 | MTR_8g009510 | 31.920 | 401 | 247 | 5 | 114 | 503 | 11 | 396 | 9.02e-53 | 193 |
| MS.gene48756.t1 | MTR_2g009690 | 22.474 | 485 | 324 | 15 | 41 | 497 | 48 | 508 | 1.26e-15 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48756.t1 | AT1G59610 | 77.088 | 934 | 186 | 11 | 1 | 920 | 1 | 920 | 0.0 | 1382 |
| MS.gene48756.t1 | AT1G10290 | 76.907 | 931 | 187 | 11 | 1 | 920 | 1 | 914 | 0.0 | 1353 |
| MS.gene48756.t1 | AT3G60190 | 31.599 | 519 | 323 | 9 | 1 | 503 | 1 | 503 | 3.94e-68 | 239 |
| MS.gene48756.t1 | AT1G14830 | 30.874 | 515 | 323 | 7 | 1 | 501 | 1 | 496 | 3.46e-66 | 234 |
| MS.gene48756.t1 | AT2G44590 | 30.814 | 516 | 319 | 10 | 4 | 501 | 1 | 496 | 5.35e-63 | 224 |
| MS.gene48756.t1 | AT2G44590 | 30.709 | 508 | 313 | 11 | 4 | 501 | 1 | 479 | 4.93e-59 | 213 |
| MS.gene48756.t1 | AT2G44590 | 30.709 | 508 | 313 | 11 | 4 | 501 | 1 | 479 | 6.42e-59 | 213 |
| MS.gene48756.t1 | AT3G61760 | 28.154 | 547 | 341 | 9 | 4 | 533 | 1 | 512 | 1.74e-58 | 211 |
| MS.gene48756.t1 | AT3G61760 | 28.154 | 547 | 341 | 9 | 4 | 533 | 1 | 512 | 1.90e-58 | 212 |
| MS.gene48756.t1 | AT5G42080 | 29.787 | 517 | 326 | 10 | 4 | 503 | 1 | 497 | 2.31e-58 | 211 |
| MS.gene48756.t1 | AT5G42080 | 29.787 | 517 | 326 | 10 | 4 | 503 | 1 | 497 | 2.31e-58 | 211 |
| MS.gene48756.t1 | AT5G42080 | 29.207 | 517 | 323 | 11 | 4 | 503 | 1 | 491 | 4.04e-54 | 199 |
| MS.gene48756.t1 | AT3G61760 | 28.358 | 469 | 289 | 11 | 4 | 450 | 1 | 444 | 2.81e-48 | 179 |
| MS.gene48756.t1 | AT5G42080 | 28.953 | 449 | 282 | 10 | 4 | 435 | 1 | 429 | 1.74e-45 | 170 |
| MS.gene48756.t1 | AT4G33650 | 22.587 | 487 | 326 | 14 | 41 | 497 | 61 | 526 | 2.89e-15 | 80.9 |
| MS.gene48756.t1 | AT4G33650 | 22.587 | 487 | 326 | 14 | 41 | 497 | 61 | 526 | 2.92e-15 | 80.9 |
| MS.gene48756.t1 | AT2G14120 | 21.660 | 494 | 323 | 15 | 41 | 497 | 45 | 511 | 2.13e-12 | 71.6 |
| MS.gene48756.t1 | AT2G14120 | 21.660 | 494 | 323 | 15 | 41 | 497 | 45 | 511 | 2.34e-12 | 71.6 |
| MS.gene48756.t1 | AT2G14120 | 21.457 | 494 | 324 | 15 | 41 | 497 | 45 | 511 | 3.68e-12 | 70.9 |
Find 238 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTGTGCCTAACCTCCTTTC+TGG | 0.133862 | 4.4:-72959151 | MS.gene48756:CDS |
| TCACCTGGTCCGCTGCTTCT+TGG | 0.181499 | 4.4:+72949384 | None:intergenic |
| TCAAGCACTTCTACTGAATA+AGG | 0.225219 | 4.4:-72959401 | MS.gene48756:CDS |
| CGTATTCCTTTGCCAATCTA+AGG | 0.250717 | 4.4:+72959638 | None:intergenic |
| TTTCATGCTTCCACCAGTTT+GGG | 0.255340 | 4.4:+72954108 | None:intergenic |
| GCCCGAGAGGAAGCAATTTC+AGG | 0.262001 | 4.4:+72959661 | None:intergenic |
| AAATCCTAACTTACCAAAAC+TGG | 0.272404 | 4.4:+72960912 | None:intergenic |
| CCTGCTCCACCAGGTTCTTC+TGG | 0.274221 | 4.4:-72949195 | MS.gene48756:CDS |
| GGAGCATAATGATGCCATTT+TGG | 0.281997 | 4.4:-72959710 | MS.gene48756:CDS |
| ATTTGGATTTGGTAGGGTTC+TGG | 0.287100 | 4.4:-72960969 | MS.gene48756:intron |
| CAACATAGCCACGGACTTCC+TGG | 0.304661 | 4.4:+72950550 | None:intergenic |
| CTACAGCCTACTGGTGAAAA+TGG | 0.307733 | 4.4:-72960810 | MS.gene48756:intron |
| ATTTCATGCTTCCACCAGTT+TGG | 0.307878 | 4.4:+72954107 | None:intergenic |
| CTCATGTTGGTTGATTTACC+TGG | 0.309222 | 4.4:-72959890 | MS.gene48756:CDS |
| GGTAATTGCTTCATTCTATC+AGG | 0.316209 | 4.4:+72957862 | None:intergenic |
| TCGTGGTGTTATTACATTAG+AGG | 0.321540 | 4.4:-72951997 | MS.gene48756:intron |
| AGGCGATGGTTTGTGCTTAA+TGG | 0.322611 | 4.4:-72952461 | MS.gene48756:CDS |
| TAAGAATGAATCAAAGAAAA+TGG | 0.322677 | 4.4:-72955998 | MS.gene48756:CDS |
| TGCTTTAAGACATTCGTTGC+AGG | 0.335841 | 4.4:-72960587 | MS.gene48756:CDS |
| ATTAACTAACTTGCTGAAAC+TGG | 0.341766 | 4.4:+72960696 | None:intergenic |
| GTACGACGGCTGCCAGAGTT+TGG | 0.342978 | 4.4:+72949234 | None:intergenic |
| TGGTACAATAACTACCAAAA+TGG | 0.348189 | 4.4:+72959696 | None:intergenic |
| CTTGCTACTGCTGCACTAGA+AGG | 0.356500 | 4.4:-72956024 | MS.gene48756:CDS |
| GCGGTGTTGAACAGTTTAAT+TGG | 0.360432 | 4.4:-72960939 | MS.gene48756:CDS |
| CAGAGCAATCTATACTTAAC+CGG | 0.360901 | 4.4:-72954294 | MS.gene48756:CDS |
| CACCTGGTCCGCTGCTTCTT+GGG | 0.361873 | 4.4:+72949385 | None:intergenic |
| AATAGTGATCCTGCACAAAA+TGG | 0.366696 | 4.4:-72949273 | MS.gene48756:CDS |
| TTCCTCTCGGGCCCTTAGAT+TGG | 0.375862 | 4.4:-72959650 | MS.gene48756:CDS |
| TTTAATTGGACATCCAGTTT+TGG | 0.376338 | 4.4:-72960925 | MS.gene48756:intron |
| AGCCGTAGTGGTTCGAGATC+TGG | 0.379668 | 4.4:-72949318 | MS.gene48756:CDS |
| TGCATAGGTACAAGAATTGT+TGG | 0.382976 | 4.4:-72959478 | MS.gene48756:intron |
| TGGAGGGGCTCCTGTCAGTA+TGG | 0.385059 | 4.4:+72959240 | None:intergenic |
| ATATCTCATTTCACCTGAAA+AGG | 0.385091 | 4.4:-72957673 | MS.gene48756:CDS |
| ATTCCCATTTGACCAATTAG+AGG | 0.385811 | 4.4:+72949889 | None:intergenic |
| ACGGCACAGTCTGGATCTTC+TGG | 0.385892 | 4.4:-72959316 | MS.gene48756:CDS |
| AAGCAAAGGGAGGACAAATA+AGG | 0.389951 | 4.4:-72951146 | MS.gene48756:CDS |
| ATCCCCTGGGTTGCATTAAT+CGG | 0.392704 | 4.4:-72959358 | MS.gene48756:CDS |
| AGGGAAGTCTCAAGTAGTTC+AGG | 0.395603 | 4.4:-72958607 | MS.gene48756:CDS |
| GCTATCCTTCCAAGCTTACT+TGG | 0.398786 | 4.4:+72959220 | None:intergenic |
| GGTGGAGGATCACTTTCATC+TGG | 0.399571 | 4.4:+72951445 | None:intergenic |
| ATTTCAGGTGCTTGAGCTGC+TGG | 0.403920 | 4.4:+72959676 | None:intergenic |
| GGCAAGGCTTGGTGAGTCAT+TGG | 0.404092 | 4.4:-72958577 | MS.gene48756:CDS |
| CAAAATGGTGACTTGAACTC+TGG | 0.405512 | 4.4:-72949258 | MS.gene48756:CDS |
| TGAAGCAATTACCGCTAGAT+AGG | 0.406607 | 4.4:-72957852 | MS.gene48756:CDS |
| TGATTCCATGCGTCAAGCTT+CGG | 0.413636 | 4.4:-72961153 | MS.gene48756:CDS |
| GGTGGTTGCACTAGTTGATA+TGG | 0.413856 | 4.4:-72955977 | MS.gene48756:CDS |
| TCAAGTAGTTCAGGATGAAT+TGG | 0.416264 | 4.4:-72958598 | MS.gene48756:CDS |
| TTGAAGTTACGCACAAATAC+AGG | 0.419757 | 4.4:-72960517 | MS.gene48756:intron |
| TACGACGGCTGCCAGAGTTT+GGG | 0.424574 | 4.4:+72949235 | None:intergenic |
| GGCGGCGATCGAAGAGTTAT+CGG | 0.428883 | 4.4:-72961183 | MS.gene48756:CDS |
| CCTGCTGTAATTTCTCCTTC+AGG | 0.429358 | 4.4:+72953995 | None:intergenic |
| CGTTGCAGATCAATCACAAT+TGG | 0.429638 | 4.4:+72960774 | None:intergenic |
| GTGCTGCTGCTGCCTCTAAT+TGG | 0.431108 | 4.4:-72949901 | MS.gene48756:CDS |
| ACTCAGTTGACGTGTCAGTT+TGG | 0.431609 | 4.4:+72949937 | None:intergenic |
| GCCAATGCTACACCTGGTTT+AGG | 0.432559 | 4.4:-72956261 | MS.gene48756:CDS |
| GAAATCACTAAAGGATGATA+AGG | 0.432708 | 4.4:-72954089 | MS.gene48756:CDS |
| GATAGCTTTGGTGGATGCTC+TGG | 0.433674 | 4.4:-72959203 | MS.gene48756:CDS |
| AAAATTGTTGCCTGCTTTGA+GGG | 0.439420 | 4.4:-72957892 | MS.gene48756:CDS |
| TCCACCAGTTTGGGGACTTG+TGG | 0.440161 | 4.4:+72954117 | None:intergenic |
| TAGCCTCTCTGATGGTTCCT+TGG | 0.440329 | 4.4:-72951091 | MS.gene48756:intron |
| GCTGCCTCTAATTGGTCAAA+TGG | 0.440464 | 4.4:-72949893 | MS.gene48756:CDS |
| TCAATAGCTACGGCACAGTC+TGG | 0.442274 | 4.4:-72959325 | MS.gene48756:CDS |
| AGTCTTATCAGTTGCACTCT+CGG | 0.447448 | 4.4:+72951200 | None:intergenic |
| AGCCCAAGAAGCAGCGGACC+AGG | 0.448731 | 4.4:-72949387 | MS.gene48756:CDS |
| CTGGTGGAGCAGGTGGTAAT+CGG | 0.450353 | 4.4:+72949205 | None:intergenic |
| GAAAATTGTTGCCTGCTTTG+AGG | 0.453558 | 4.4:-72957893 | MS.gene48756:CDS |
| GTGTGCCTAACCTCCTTTCT+GGG | 0.459672 | 4.4:-72959150 | MS.gene48756:intron |
| TCACCACTTCAGAGGGTACT+CGG | 0.460450 | 4.4:-72958554 | MS.gene48756:CDS |
| GCAGCGGACCAGGTGATGAC+TGG | 0.462994 | 4.4:-72949377 | MS.gene48756:CDS |
| CCTTATGCTGTAGGTACATC+GGG | 0.464070 | 4.4:-72956310 | MS.gene48756:intron |
| CCAGAAGAACCTGGTGGAGC+AGG | 0.466061 | 4.4:+72949195 | None:intergenic |
| TTGAACTCTGGCCCAAACTC+TGG | 0.469406 | 4.4:-72949246 | MS.gene48756:CDS |
| ACTAAAGGATGATAAGGATA+AGG | 0.469503 | 4.4:-72954083 | MS.gene48756:CDS |
| ACCTGAAATTGCTTCCTCTC+GGG | 0.469864 | 4.4:-72959662 | MS.gene48756:CDS |
| CATCCATCGGAGTTCTTCCT+CGG | 0.472764 | 4.4:+72950575 | None:intergenic |
| GACCCTTCAACTCATCTTCT+CGG | 0.475446 | 4.4:+72954341 | None:intergenic |
| GGCTCTGGTTTAAAGTCTGC+TGG | 0.475807 | 4.4:-72954019 | MS.gene48756:CDS |
| ATCAATTATATAGCTCTGTC+AGG | 0.478411 | 4.4:-72950346 | MS.gene48756:intron |
| AGTTCTTCCTCGGGATCAGC+AGG | 0.478429 | 4.4:+72950585 | None:intergenic |
| CACCTGAAATTGCTTCCTCT+CGG | 0.478690 | 4.4:-72959663 | MS.gene48756:CDS |
| ACCACAACACTTCATTCGCT+TGG | 0.479418 | 4.4:-72955938 | MS.gene48756:CDS |
| TCCTTCCAAGCTTACTTGGA+GGG | 0.482325 | 4.4:+72959224 | None:intergenic |
| CAGGATGAATTGGCAAGGCT+TGG | 0.486330 | 4.4:-72958588 | MS.gene48756:CDS |
| AGCGATGACTTTGAGAAAAG+TGG | 0.487707 | 4.4:+72961076 | None:intergenic |
| TAGGCCACAAGTCCCCAAAC+TGG | 0.488346 | 4.4:-72954121 | MS.gene48756:CDS |
| TTTATTTATTTAGGATGGAA+AGG | 0.489100 | 4.4:-72954369 | MS.gene48756:intron |
| GTTGGTTGATTTACCTGGGT+TGG | 0.489726 | 4.4:-72959885 | MS.gene48756:CDS |
| TTGATATCAACAATGTAAAG+AGG | 0.493127 | 4.4:-72957825 | MS.gene48756:CDS |
| GGATGATAAGGATAAGGAAA+AGG | 0.495935 | 4.4:-72954077 | MS.gene48756:CDS |
| CTGCCTCTAATTGGTCAAAT+GGG | 0.495988 | 4.4:-72949892 | MS.gene48756:CDS |
| GGAGCAGGTGGTAATCGGTT+AGG | 0.496932 | 4.4:+72949210 | None:intergenic |
| TCATGTTGGTTGATTTACCT+GGG | 0.497908 | 4.4:-72959889 | MS.gene48756:CDS |
| AAAGAAGGGCAAGAAGGCTC+TGG | 0.498172 | 4.4:-72954034 | MS.gene48756:CDS |
| TAAGCTTGGAAGGATAGCTT+TGG | 0.499811 | 4.4:-72959215 | MS.gene48756:CDS |
| AATTCTATATGGCAGGGAAC+GGG | 0.500065 | 4.4:-72957919 | MS.gene48756:intron |
| CAAGCACTTCTACTGAATAA+GGG | 0.500607 | 4.4:-72959400 | MS.gene48756:CDS |
| GGTTCGAGATCTGGATCAAA+TGG | 0.501374 | 4.4:-72949309 | MS.gene48756:CDS |
| GCTTGGAAGGATAGCTTTGG+TGG | 0.501389 | 4.4:-72959212 | MS.gene48756:CDS |
| TCCTTATGCTGTAGGTACAT+CGG | 0.501705 | 4.4:-72956311 | MS.gene48756:intron |
| GGAAAAGGACAAGTCAGGTC+AGG | 0.503566 | 4.4:-72954062 | MS.gene48756:CDS |
| CCCAGCACCACCTCTCATGT+TGG | 0.505341 | 4.4:-72959903 | MS.gene48756:intron |
| ATCCTTCCAAGCTTACTTGG+AGG | 0.506008 | 4.4:+72959223 | None:intergenic |
| CCTTCCAAGCTTACTTGGAG+GGG | 0.506373 | 4.4:+72959225 | None:intergenic |
| TCAGGTCAGGCAGAGAAAGA+AGG | 0.508013 | 4.4:-72954049 | MS.gene48756:CDS |
| GATATATTGTTCAGGCTACA+AGG | 0.509066 | 4.4:-72958627 | MS.gene48756:intron |
| TTTGTGCTTAATGGAAAGAC+AGG | 0.511215 | 4.4:-72952452 | MS.gene48756:CDS |
| AACATACCTCGCCTGAGGTA+AGG | 0.512008 | 4.4:+72958481 | None:intergenic |
| GATAAGGAAAAGGACAAGTC+AGG | 0.512725 | 4.4:-72954067 | MS.gene48756:CDS |
| TATCTCATTTCACCTGAAAA+GGG | 0.512750 | 4.4:-72957672 | MS.gene48756:CDS |
| TTGCTACTGCTGCACTAGAA+GGG | 0.516312 | 4.4:-72956023 | MS.gene48756:CDS |
| ACGCCGAGAAGATGAGTTGA+AGG | 0.517163 | 4.4:-72954344 | MS.gene48756:CDS |
| GCCATTGCTAGCTGCATCAA+AGG | 0.518187 | 4.4:+72949347 | None:intergenic |
| TAAGACATTCGTTGCAGGAT+AGG | 0.518204 | 4.4:-72960582 | MS.gene48756:CDS |
| GGAGCCCCTCCAAGTAAGCT+TGG | 0.519195 | 4.4:-72959229 | MS.gene48756:CDS |
| TTCCTACAGCACCTTACCTC+AGG | 0.521850 | 4.4:-72958492 | MS.gene48756:CDS |
| CAAAAGCACAAGATATCCCC+TGG | 0.522777 | 4.4:-72959372 | MS.gene48756:CDS |
| AACATAGCCACGGACTTCCT+GGG | 0.524261 | 4.4:+72950551 | None:intergenic |
| TCCTAAACCAGGTGTAGCAT+TGG | 0.525175 | 4.4:+72956260 | None:intergenic |
| TTTCTCAAAGTCATCGCTCT+CGG | 0.526629 | 4.4:-72961071 | MS.gene48756:CDS |
| ACAAATAAGGCTTTCATCTG+AGG | 0.527017 | 4.4:-72951133 | MS.gene48756:CDS |
| CAGGTCAGGCAGAGAAAGAA+GGG | 0.528176 | 4.4:-72954048 | MS.gene48756:CDS |
| CTCCTGTCAGTATGGACTTG+AGG | 0.528269 | 4.4:+72959248 | None:intergenic |
| GCCTTTGATGCAGCTAGCAA+TGG | 0.529781 | 4.4:-72949348 | MS.gene48756:CDS |
| AATTGGTCAAATGGGAATGC+AGG | 0.531196 | 4.4:-72949884 | MS.gene48756:intron |
| AGTAATGTTATACAAGCAAA+GGG | 0.532719 | 4.4:-72951159 | MS.gene48756:CDS |
| AAGTAATGTTATACAAGCAA+AGG | 0.534178 | 4.4:-72951160 | MS.gene48756:CDS |
| AAAATTCTTTAGAAACTGCA+TGG | 0.534480 | 4.4:-72959288 | MS.gene48756:CDS |
| GTGCAACTGATAAGACTGAA+TGG | 0.537651 | 4.4:-72951194 | MS.gene48756:CDS |
| AGGAAATCTCCCCTCAAAGC+AGG | 0.538072 | 4.4:+72957882 | None:intergenic |
| GTAGGTACATCGGGTCCTGA+TGG | 0.538683 | 4.4:-72956301 | MS.gene48756:intron |
| CGGCCGATACAATATCCATC+AGG | 0.538890 | 4.4:+72956286 | None:intergenic |
| CCCCTCCAAGTAAGCTTGGA+AGG | 0.541389 | 4.4:-72959225 | MS.gene48756:CDS |
| TTAAAGTCTGCTGGTCCTGA+AGG | 0.542508 | 4.4:-72954010 | MS.gene48756:CDS |
| AGGCTGACTTTGCTAGAGTC+CGG | 0.542530 | 4.4:+72951391 | None:intergenic |
| AGCCTCAAGTCCATACTGAC+AGG | 0.543699 | 4.4:-72959250 | MS.gene48756:CDS |
| GGGTACTCGGGCTATAGCCT+TGG | 0.544929 | 4.4:-72958541 | MS.gene48756:CDS |
| CAGATTGTGTTAGAAGCCGA+TGG | 0.548468 | 4.4:-72957705 | MS.gene48756:CDS |
| ATCCATCGGAGTTCTTCCTC+GGG | 0.548958 | 4.4:+72950576 | None:intergenic |
| CTTCTAGTGCAGCAGTAGCA+AGG | 0.551079 | 4.4:+72956025 | None:intergenic |
| CTCATCTCGAGCTTTGGCAG+AGG | 0.553204 | 4.4:+72960546 | None:intergenic |
| AATGGCTCTGTTAGCCGTAG+TGG | 0.555895 | 4.4:-72949330 | MS.gene48756:CDS |
| CTAATGGTTGGAGCAGGCGA+TGG | 0.556957 | 4.4:-72952475 | MS.gene48756:CDS |
| GTATTCCTTTGCCAATCTAA+GGG | 0.557791 | 4.4:+72959639 | None:intergenic |
| AGCATGAAAATTACCCAGAA+AGG | 0.557821 | 4.4:+72959137 | None:intergenic |
| CGATTACCACCTGCTCCACC+AGG | 0.559073 | 4.4:-72949204 | MS.gene48756:CDS |
| ACTTCATTCGCTTGGTACAA+AGG | 0.559825 | 4.4:-72955930 | MS.gene48756:CDS |
| CATTATTAGTGGTCAAAGCA+CGG | 0.561531 | 4.4:-72950041 | MS.gene48756:intron |
| GCCGCTGCCAATGCTACACC+TGG | 0.565575 | 4.4:-72956267 | MS.gene48756:CDS |
| TTCATGCTTCCACCAGTTTG+GGG | 0.565736 | 4.4:+72954109 | None:intergenic |
| AAGATCAAAATGTAAAGCGC+AGG | 0.565812 | 4.4:-72949994 | MS.gene48756:CDS |
| TCAAAGCACGGCTAAGATTG+AGG | 0.565979 | 4.4:-72950029 | MS.gene48756:CDS |
| CCTGAAGGAGAAATTACAGC+AGG | 0.566736 | 4.4:-72953995 | MS.gene48756:intron |
| GGACTTCCTGGGACATCCAT+CGG | 0.567523 | 4.4:+72950562 | None:intergenic |
| AAACTCACGGCAAAGTTCCA+AGG | 0.568497 | 4.4:+72958524 | None:intergenic |
| TGGGTTGGATCAGCGCATCA+TGG | 0.569659 | 4.4:-72959870 | MS.gene48756:CDS |
| TGATATCAACAATGTAAAGA+GGG | 0.572653 | 4.4:-72957824 | MS.gene48756:intron |
| CACCACTTCAGAGGGTACTC+GGG | 0.573369 | 4.4:-72958553 | MS.gene48756:CDS |
| TTCTAGTGCAGCAGTAGCAA+GGG | 0.573659 | 4.4:+72956026 | None:intergenic |
| TCAATCAGTTTCAATAGCTA+CGG | 0.576013 | 4.4:-72959335 | MS.gene48756:CDS |
| GCAGAGAAAGAAGGGCAAGA+AGG | 0.576545 | 4.4:-72954040 | MS.gene48756:CDS |
| TACCATACCTCTCTCTTGAA+AGG | 0.578019 | 4.4:+72956228 | None:intergenic |
| AATTACCCAGAAAGGAGGTT+AGG | 0.578066 | 4.4:+72959145 | None:intergenic |
| CATACCTCTCTCTTGAAAGG+TGG | 0.578115 | 4.4:+72956231 | None:intergenic |
| ATCCCGAGGAAGAACTCCGA+TGG | 0.578121 | 4.4:-72950578 | MS.gene48756:CDS |
| TGACCGATTAATGCAACCCA+GGG | 0.578753 | 4.4:+72959355 | None:intergenic |
| TAACTCTTCGATCGCCGCCA+TGG | 0.578909 | 4.4:+72961187 | None:intergenic |
| TGGAAGCATGAAATCACTAA+AGG | 0.579776 | 4.4:-72954098 | MS.gene48756:CDS |
| ACCAGGTGTAGCATTGGCAG+CGG | 0.585419 | 4.4:+72956266 | None:intergenic |
| ATACCTCTCTCTTGAAAGGT+GGG | 0.586459 | 4.4:+72956232 | None:intergenic |
| GGTCCTGATGGATATTGTAT+CGG | 0.586959 | 4.4:-72956289 | MS.gene48756:CDS |
| GTTGCAGGATAGGTTGAGCA+AGG | 0.588653 | 4.4:-72960572 | MS.gene48756:CDS |
| ATCCAGATCTCGAACCACTA+CGG | 0.591987 | 4.4:+72949316 | None:intergenic |
| GCAGTTCTCCAGTCATCACC+TGG | 0.592447 | 4.4:+72949369 | None:intergenic |
| GGACAAGAAGTCTAACGGAC+CGG | 0.595221 | 4.4:-72951410 | MS.gene48756:CDS |
| GGAACTTATCTTCAAACTCA+CGG | 0.595319 | 4.4:+72958511 | None:intergenic |
| GCAAAGGAATACGACGGAGA+AGG | 0.596380 | 4.4:-72959628 | MS.gene48756:intron |
| AAGCGCCGAAGCTTGACGCA+TGG | 0.598831 | 4.4:+72961148 | None:intergenic |
| CAAATAAGGCTTTCATCTGA+GGG | 0.600814 | 4.4:-72951132 | MS.gene48756:CDS |
| CGCCTGAGGTAAGGTGCTGT+AGG | 0.601487 | 4.4:+72958490 | None:intergenic |
| ATCACGTCTTTGTGTTGATG+AGG | 0.601611 | 4.4:-72957604 | MS.gene48756:intron |
| GCAGGAATGTAACATTGAAG+AGG | 0.601736 | 4.4:-72951470 | MS.gene48756:intron |
| TCGAAGACCTGCTGATCCCG+AGG | 0.602524 | 4.4:-72950592 | MS.gene48756:CDS |
| TCGGGCCCTTAGATTGGCAA+AGG | 0.604108 | 4.4:-72959644 | MS.gene48756:CDS |
| GTCATTGGTCACCACTTCAG+AGG | 0.606331 | 4.4:-72958562 | MS.gene48756:CDS |
| AGAACTCCGATGGATGTCCC+AGG | 0.607765 | 4.4:-72950568 | MS.gene48756:CDS |
| GAGATATGGCTGATAACCAT+CGG | 0.609703 | 4.4:+72957689 | None:intergenic |
| GAGAGTAGCCCAAGAAGCAG+CGG | 0.610996 | 4.4:-72949393 | MS.gene48756:CDS |
| TTTAGCTGCTAATGTACCAA+AGG | 0.615126 | 4.4:-72950511 | MS.gene48756:intron |
| AGCCCGAGTACCCTCTGAAG+TGG | 0.615653 | 4.4:+72958551 | None:intergenic |
| ATGAAAATTACCCAGAAAGG+AGG | 0.618579 | 4.4:+72959140 | None:intergenic |
| GAAGAACCTGGTGGAGCAGG+TGG | 0.619073 | 4.4:+72949198 | None:intergenic |
| GGATTAAGATCACTAATCAA+AGG | 0.620131 | 4.4:-72957651 | MS.gene48756:CDS |
| CTATATGGCAGGGAACGGGT+TGG | 0.621898 | 4.4:-72957915 | MS.gene48756:intron |
| GCCACAAGTCCCCAAACTGG+TGG | 0.623441 | 4.4:-72954118 | MS.gene48756:CDS |
| TGTCCTAACTCAGGATACAA+TGG | 0.623479 | 4.4:-72950616 | MS.gene48756:intron |
| AAACTGCTTCAACATAGCCA+CGG | 0.625255 | 4.4:+72950541 | None:intergenic |
| TCAAAGGACAAGAAGTCTAA+CGG | 0.625535 | 4.4:-72951415 | MS.gene48756:CDS |
| AGTTTCGTCGATATCTTCGT+CGG | 0.626560 | 4.4:+72961121 | None:intergenic |
| TTGACCGATTAATGCAACCC+AGG | 0.627576 | 4.4:+72959354 | None:intergenic |
| CTGAGGGTGGCTCAGCGATG+AGG | 0.627639 | 4.4:-72951116 | MS.gene48756:CDS |
| TGTATAGGTTAGTGAATATG+CGG | 0.628140 | 4.4:-72959731 | MS.gene48756:intron |
| TAGTTCAGGATGAATTGGCA+AGG | 0.632684 | 4.4:-72958593 | MS.gene48756:CDS |
| ATATATTGTTCAGGCTACAA+GGG | 0.632739 | 4.4:-72958626 | MS.gene48756:intron |
| GAATGAATCAAAGAAAATGG+TGG | 0.633340 | 4.4:-72955995 | MS.gene48756:CDS |
| TCATCAACACAAAGACGTGA+TGG | 0.633705 | 4.4:+72957606 | None:intergenic |
| TCATTGGTCACCACTTCAGA+GGG | 0.644736 | 4.4:-72958561 | MS.gene48756:CDS |
| AGAGCAATCTATACTTAACC+GGG | 0.645583 | 4.4:-72954293 | MS.gene48756:intron |
| GGTGGGTATCTTCCTAAACC+AGG | 0.649101 | 4.4:+72956249 | None:intergenic |
| ATACCCACCTTTCAAGAGAG+AGG | 0.650832 | 4.4:-72956235 | MS.gene48756:intron |
| GCTTAATGGAAAGACAGGAA+AGG | 0.651090 | 4.4:-72952447 | MS.gene48756:intron |
| GTAGGGTTCTGGTAAATCAG+CGG | 0.653243 | 4.4:-72960958 | MS.gene48756:intron |
| ATGAGGCATAGCCTCTCTGA+TGG | 0.656381 | 4.4:-72951099 | MS.gene48756:CDS |
| TGCCAATCTAAGGGCCCGAG+AGG | 0.658116 | 4.4:+72959648 | None:intergenic |
| GGTAAATCAACCAACATGAG+AGG | 0.666994 | 4.4:+72959893 | None:intergenic |
| ATAAGGCTTTCATCTGAGGG+TGG | 0.667152 | 4.4:-72951129 | MS.gene48756:CDS |
| ATATCAAAATGCCTATCTAG+CGG | 0.667297 | 4.4:+72957841 | None:intergenic |
| AATTAGAGGCAGCAGCAGCA+CGG | 0.667388 | 4.4:+72949903 | None:intergenic |
| TATCTGTAACCAGAAGAACC+TGG | 0.668384 | 4.4:+72949186 | None:intergenic |
| AAATTGTTGCCTGCTTTGAG+GGG | 0.669662 | 4.4:-72957891 | MS.gene48756:CDS |
| CGCCGAGAAGATGAGTTGAA+GGG | 0.670684 | 4.4:-72954343 | MS.gene48756:CDS |
| AAATCAACCAACATGAGAGG+TGG | 0.675516 | 4.4:+72959896 | None:intergenic |
| TGTCAGTTTGGAGAGAAGCG+AGG | 0.676009 | 4.4:+72949949 | None:intergenic |
| ATGACTTTGAGAAAAGTGGA+AGG | 0.677400 | 4.4:+72961080 | None:intergenic |
| AAAAGCACAAGATATCCCCT+GGG | 0.679013 | 4.4:-72959371 | MS.gene48756:CDS |
| GAGAAGATGAGTTGAAGGGT+CGG | 0.679267 | 4.4:-72954339 | MS.gene48756:CDS |
| TCATTCGCTTGGTACAAAGG+AGG | 0.682719 | 4.4:-72955927 | MS.gene48756:intron |
| AATGTTATACAAGCAAAGGG+AGG | 0.683984 | 4.4:-72951156 | MS.gene48756:CDS |
| ATGATGCGCTGATCCAACCC+AGG | 0.686647 | 4.4:+72959872 | None:intergenic |
| TGGATGTCCCAGGAAGTCCG+TGG | 0.693140 | 4.4:-72950558 | MS.gene48756:CDS |
| ACAGCACCTTACCTCAGGCG+AGG | 0.694608 | 4.4:-72958487 | MS.gene48756:intron |
| GAGGCAACATACCTCGCCTG+AGG | 0.701371 | 4.4:+72958476 | None:intergenic |
| CTGTAACCAGAAGAACCTGG+TGG | 0.714812 | 4.4:+72949189 | None:intergenic |
| ACCAAGCGAATGAAGTGTTG+TGG | 0.731513 | 4.4:+72955937 | None:intergenic |
| AAGCGAATGAAGTGTTGTGG+TGG | 0.733403 | 4.4:+72955940 | None:intergenic |
| GACCGATTAATGCAACCCAG+GGG | 0.767187 | 4.4:+72959356 | None:intergenic |
| AGATTGGCAAAGGAATACGA+CGG | 0.768412 | 4.4:-72959634 | MS.gene48756:CDS |
| TCACCAAGGAACCATCAGAG+AGG | 0.775251 | 4.4:+72951088 | None:intergenic |
| GTAATCGGTTAGGAGTACGA+CGG | 0.815501 | 4.4:+72949220 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 72949182 | 72961207 | 72949182 | ID=MS.gene48756 |
| chr4.4 | mRNA | 72949182 | 72961207 | 72949182 | ID=MS.gene48756.t1;Parent=MS.gene48756 |
| chr4.4 | exon | 72961064 | 72961207 | 72961064 | ID=MS.gene48756.t1.exon1;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72961064 | 72961207 | 72961064 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72960926 | 72960976 | 72960926 | ID=MS.gene48756.t1.exon2;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72960926 | 72960976 | 72960926 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72960706 | 72960826 | 72960706 | ID=MS.gene48756.t1.exon3;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72960706 | 72960826 | 72960706 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72960518 | 72960610 | 72960518 | ID=MS.gene48756.t1.exon4;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72960518 | 72960610 | 72960518 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72959859 | 72959920 | 72959859 | ID=MS.gene48756.t1.exon5;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72959859 | 72959920 | 72959859 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72959629 | 72959746 | 72959629 | ID=MS.gene48756.t1.exon6;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72959629 | 72959746 | 72959629 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72959151 | 72959493 | 72959151 | ID=MS.gene48756.t1.exon7;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72959151 | 72959493 | 72959151 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72958488 | 72958635 | 72958488 | ID=MS.gene48756.t1.exon8;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72958488 | 72958635 | 72958488 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72957825 | 72957926 | 72957825 | ID=MS.gene48756.t1.exon9;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72957825 | 72957926 | 72957825 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72957605 | 72957724 | 72957605 | ID=MS.gene48756.t1.exon10;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72957605 | 72957724 | 72957605 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72956236 | 72956319 | 72956236 | ID=MS.gene48756.t1.exon11;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72956236 | 72956319 | 72956236 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72955928 | 72956055 | 72955928 | ID=MS.gene48756.t1.exon12;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72955928 | 72956055 | 72955928 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72954294 | 72954378 | 72954294 | ID=MS.gene48756.t1.exon13;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72954294 | 72954378 | 72954294 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72953996 | 72954140 | 72953996 | ID=MS.gene48756.t1.exon14;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72953996 | 72954140 | 72953996 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72952448 | 72952524 | 72952448 | ID=MS.gene48756.t1.exon15;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72952448 | 72952524 | 72952448 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72951998 | 72952054 | 72951998 | ID=MS.gene48756.t1.exon16;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72951998 | 72952054 | 72951998 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72951344 | 72951488 | 72951344 | ID=MS.gene48756.t1.exon17;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72951344 | 72951488 | 72951344 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72951092 | 72951246 | 72951092 | ID=MS.gene48756.t1.exon18;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72951092 | 72951246 | 72951092 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72950512 | 72950625 | 72950512 | ID=MS.gene48756.t1.exon19;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72950512 | 72950625 | 72950512 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72950347 | 72950414 | 72950347 | ID=MS.gene48756.t1.exon20;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72950347 | 72950414 | 72950347 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72949885 | 72950054 | 72949885 | ID=MS.gene48756.t1.exon21;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72949885 | 72950054 | 72949885 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| chr4.4 | exon | 72949182 | 72949414 | 72949182 | ID=MS.gene48756.t1.exon22;Parent=MS.gene48756.t1 |
| chr4.4 | CDS | 72949182 | 72949414 | 72949182 | ID=cds.MS.gene48756.t1;Parent=MS.gene48756.t1 |
| Gene Sequence |
| Protein sequence |
