AlfalfaGEDB | MS.gene48841

M. sativa cultivar XinJiangDaYe / MS.gene48841

KEGG: K09481
GO: GO:0005575 GO:0005784 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0032991 GO:0033036 GO:0042886 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071256 GO:0071702 GO:0071705 GO:0098796
PFAM: PF03911.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48841.t1	XP_003605285.1	100	43	0	0	38	80	38	80	2.10E-13	84.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48841.t1	P38389	94.7	38	2	0	39	76	42	79	5.2e-13	74.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48841.t1	G7JIA6	100.0	43	0	0	38	80	38	80	1.5e-13	84.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene48841	0.823748	1.17E-53	-1.69E-46
MS.gene04920	MS.gene48841	0.829776	4.26E-55	-1.69E-46
MS.gene049283	MS.gene48841	0.80207	6.68E-49	-1.69E-46
MS.gene049680	MS.gene48841	0.80179	7.62E-49	-1.69E-46
MS.gene050955	MS.gene48841	0.800991	1.11E-48	-1.69E-46
MS.gene051003	MS.gene48841	0.837483	5.06E-57	-1.69E-46
MS.gene051343	MS.gene48841	0.822529	2.26E-53	-1.69E-46
MS.gene051597	MS.gene48841	0.818913	1.53E-52	-1.69E-46
MS.gene05188	MS.gene48841	0.821258	4.44E-53	-1.69E-46
MS.gene051975	MS.gene48841	0.846677	1.87E-59	-1.69E-46
MS.gene052889	MS.gene48841	0.807969	3.90E-50	-1.69E-46
MS.gene053058	MS.gene48841	0.816019	6.86E-52	-1.69E-46
MS.gene053564	MS.gene48841	0.818883	1.55E-52	-1.69E-46
MS.gene05517	MS.gene48841	0.802798	4.73E-49	-1.69E-46
MS.gene055513	MS.gene48841	0.803251	3.81E-49	-1.69E-46
MS.gene056631	MS.gene48841	0.805687	1.18E-49	-1.69E-46
MS.gene056699	MS.gene48841	0.807545	4.80E-50	-1.69E-46
MS.gene056986	MS.gene48841	0.847048	1.48E-59	-1.69E-46
MS.gene058708	MS.gene48841	-0.833753	4.45E-56	-1.69E-46
MS.gene059146	MS.gene48841	0.84016	1.03E-57	-1.69E-46
MS.gene059901	MS.gene48841	-0.830506	2.83E-55	-1.69E-46
MS.gene06011	MS.gene48841	0.827681	1.37E-54	-1.69E-46
MS.gene060365	MS.gene48841	0.803722	3.04E-49	-1.69E-46
MS.gene060429	MS.gene48841	0.805809	1.12E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48841.t1	MTR_4g028080	98.750	80	1	0	1	80	1	80	2.74e-51	155
MS.gene48841.t1	MTR_8g086330	93.671	79	5	0	1	79	1	79	1.49e-41	130
MS.gene48841.t1	MTR_1g082400	59.494	79	24	1	10	80	26	104	2.12e-24	88.6
MS.gene48841.t1	MTR_2g093770	60.241	83	27	1	4	80	21	103	1.16e-23	86.3
MS.gene48841.t1	MTR_5g066500	66.071	56	16	1	9	64	56	108	1.25e-15	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene48841.t1	AT3G60540	84.211	76	11	1	3	77	2	77	6.04e-35	114
MS.gene48841.t1	AT3G60540	84.211	76	11	1	3	77	2	77	6.04e-35	114
MS.gene48841.t1	AT3G60540	84.211	76	11	1	3	77	2	77	6.04e-35	114
MS.gene48841.t1	AT2G45070	84.810	79	8	1	3	77	2	80	4.90e-33	109
MS.gene48841.t1	AT2G45070	84.810	79	8	1	3	77	2	80	4.90e-33	109
MS.gene48841.t1	AT2G45070	84.810	79	8	1	3	77	2	80	4.90e-33	109
MS.gene48841.t1	AT2G45070	84.810	79	8	1	3	77	2	80	4.90e-33	109
MS.gene48841.t1	AT5G60460	58.333	84	27	1	5	80	22	105	1.69e-22	83.6
MS.gene48841.t1	AT5G60460	58.333	84	27	1	5	80	22	105	1.69e-22	83.6

Find 29 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTGCAGCACTTCCTCTTT+GGG	0.197645	4.4:+74304396	None:intergenic
TAGCTGCAGCACTTCCTCTT+TGG	0.251750	4.4:+74304395	None:intergenic
ACACCAGGTGGTGGCGCCTC+TGG	0.332072	4.4:-74304349	MS.gene48841:CDS
TCATTTCAGAAAATGGCTTT+AGG	0.348674	4.4:-74304430	None:intergenic
GGCGCCTCTGGAGGAGCAGC+TGG	0.387947	4.4:-74304337	MS.gene48841:CDS
CCTCCAGAGGCGCCACCACC+TGG	0.393423	4.4:+74304346	None:intergenic
TGTACTTGAGGGAGGCTTAA+GGG	0.407052	4.4:-74304195	None:intergenic
CTGTACTTGAGGGAGGCTTA+AGG	0.429114	4.4:-74304196	None:intergenic
CAGAAAATGGCTTTAGGTGG+AGG	0.431685	4.4:-74304424	None:intergenic
CGTGGGAAAGCTGTACTTGA+GGG	0.469860	4.4:-74304206	MS.gene48841:CDS
CTCATCACAAGAACAACATT+AGG	0.475458	4.4:+74304265	None:intergenic
GTTGTTCTTGTGATGAGCAT+TGG	0.493707	4.4:-74304259	MS.gene48841:CDS
TGTTGCCGTCCTTCATGTCG+TGG	0.499572	4.4:-74304224	MS.gene48841:CDS
GCTGCAGCACTTCCTCTTTG+GGG	0.514244	4.4:+74304397	None:intergenic
CTACTGCAAGCATGAGGAGA+AGG	0.536447	4.4:-74304375	MS.gene48841:CDS
GTTGCCGTCCTTCATGTCGT+GGG	0.536584	4.4:-74304223	MS.gene48841:CDS
CCAGGTGGTGGCGCCTCTGG+AGG	0.542338	4.4:-74304346	MS.gene48841:CDS
TTTCAGAAAATGGCTTTAGG+TGG	0.554792	4.4:-74304427	None:intergenic
TTTGAGTCCAGGTGCATCGT+CGG	0.570781	4.4:+74304294	None:intergenic
AGAAGGAAAACACCAGGTGG+TGG	0.575500	4.4:-74304358	MS.gene48841:CDS
AGTTCCAGCTGCTCCTCCAG+AGG	0.584930	4.4:+74304333	None:intergenic
GGTGGAGGAGCTCCCCAAAG+AGG	0.606488	4.4:-74304409	MS.gene48841:CDS
ACAGCTTTCCCACGACATGA+AGG	0.608814	4.4:+74304215	None:intergenic
TCGTGGGAAAGCTGTACTTG+AGG	0.624214	4.4:-74304207	MS.gene48841:CDS
CTGCAGCTACTGCAAGCATG+AGG	0.624920	4.4:-74304381	MS.gene48841:CDS
ATGAGGAGAAGGAAAACACC+AGG	0.632567	4.4:-74304364	MS.gene48841:CDS
CTTTCCCACGACATGAAGGA+CGG	0.643668	4.4:+74304219	None:intergenic
GGGAAAGCTGTACTTGAGGG+AGG	0.648270	4.4:-74304203	MS.gene48841:CDS
AGGAGAAGGAAAACACCAGG+TGG	0.731216	4.4:-74304361	MS.gene48841:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CTCATCACAAGAACAACATT+AGG	+	chr4.4:74304354-74304373	None:intergenic	35.0%
	GTTGTTCTTGTGATGAGCAT+TGG	-	chr4.4:74304357-74304376	MS.gene48841:CDS	40.0%
!!	TTAGGTGAGATTTTGAGTCC+AGG	+	chr4.4:74304336-74304355	None:intergenic	40.0%
	ATGAGGAGAAGGAAAACACC+AGG	-	chr4.4:74304252-74304271	MS.gene48841:CDS	45.0%
	ACAGCTTTCCCACGACATGA+AGG	+	chr4.4:74304404-74304423	None:intergenic	50.0%
	AGAAGGAAAACACCAGGTGG+TGG	-	chr4.4:74304258-74304277	MS.gene48841:CDS	50.0%
	AGCTGCAGCACTTCCTCTTT+GGG	+	chr4.4:74304223-74304242	None:intergenic	50.0%
	AGGAGAAGGAAAACACCAGG+TGG	-	chr4.4:74304255-74304274	MS.gene48841:CDS	50.0%
	CGTGGGAAAGCTGTACTTGA+GGG	-	chr4.4:74304410-74304429	MS.gene48841:CDS	50.0%
	CTACTGCAAGCATGAGGAGA+AGG	-	chr4.4:74304241-74304260	MS.gene48841:CDS	50.0%
	CTTTCCCACGACATGAAGGA+CGG	+	chr4.4:74304400-74304419	None:intergenic	50.0%
	TAGCTGCAGCACTTCCTCTT+TGG	+	chr4.4:74304224-74304243	None:intergenic	50.0%
	TCGTGGGAAAGCTGTACTTG+AGG	-	chr4.4:74304409-74304428	MS.gene48841:CDS	50.0%
	TTTGAGTCCAGGTGCATCGT+CGG	+	chr4.4:74304325-74304344	None:intergenic	50.0%
!	TTTTACACCGACGATGCACC+TGG	-	chr4.4:74304315-74304334	MS.gene48841:CDS	50.0%
	CTGCAGCTACTGCAAGCATG+AGG	-	chr4.4:74304235-74304254	MS.gene48841:CDS	55.0%
	GCTGCAGCACTTCCTCTTTG+GGG	+	chr4.4:74304222-74304241	None:intergenic	55.0%
	GGGAAAGCTGTACTTGAGGG+AGG	-	chr4.4:74304413-74304432	MS.gene48841:CDS	55.0%
	GTTGCCGTCCTTCATGTCGT+GGG	-	chr4.4:74304393-74304412	MS.gene48841:CDS	55.0%
	TGTTGCCGTCCTTCATGTCG+TGG	-	chr4.4:74304392-74304411	MS.gene48841:CDS	55.0%
	AGTTCCAGCTGCTCCTCCAG+AGG	+	chr4.4:74304286-74304305	None:intergenic	60.0%
	GGTGGAGGAGCTCCCCAAAG+AGG	-	chr4.4:74304207-74304226	MS.gene48841:CDS	65.0%
!	ACACCAGGTGGTGGCGCCTC+TGG	-	chr4.4:74304267-74304286	MS.gene48841:CDS	70.0%
!	CCAGGTGGTGGCGCCTCTGG+AGG	-	chr4.4:74304270-74304289	MS.gene48841:CDS	75.0%
!	GGCGCCTCTGGAGGAGCAGC+TGG	-	chr4.4:74304279-74304298	MS.gene48841:CDS	75.0%
!!	CCTCCAGAGGCGCCACCACC+TGG	+	chr4.4:74304273-74304292	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	74304198	74304440	74304198	ID=MS.gene48841
chr4.4	mRNA	74304198	74304440	74304198	ID=MS.gene48841.t1;Parent=MS.gene48841
chr4.4	exon	74304198	74304440	74304198	ID=MS.gene48841.t1.exon1;Parent=MS.gene48841.t1
chr4.4	CDS	74304198	74304440	74304198	ID=cds.MS.gene48841.t1;Parent=MS.gene48841.t1

Gene Sequence	>MS.gene48841 ATGGCTTTAGGTGGAGGAGCTCCCCAAAGAGGAAGTGCTGCAGCTACTGCAAGCATGAGGAGAAGGAAAACACCAGGTGGTGGCGCCTCTGGAGGAGCAGCTGGAACTATGCTTCAATTTTACACCGACGATGCACCTGGACTCAAAATCTCACCTAATGTTGTTCTTGTGATGAGCATTGGCTTTATAGCTTTTGTTGCCGTCCTTCATGTCGTGGGAAAGCTGTACTTGAGGGAGGCTTAA
Protein sequence	>MS.gene48841.t1 MALGGGAPQRGSAAATASMRRRKTPGGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMSIGFIAFVAVLHVVGKLYLREA